CN110476744A - Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area - Google Patents

Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area Download PDF

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Publication number
CN110476744A
CN110476744A CN201910879142.2A CN201910879142A CN110476744A CN 110476744 A CN110476744 A CN 110476744A CN 201910879142 A CN201910879142 A CN 201910879142A CN 110476744 A CN110476744 A CN 110476744A
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rape
leaf
disease
yield
resistant anti
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王汉中
顿小玲
刘贵华
王新发
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Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
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Oil Crops Research Institute of Chinese Academy of Agriculture Sciences
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Priority to CN201910879142.2A priority Critical patent/CN110476744A/en
Publication of CN110476744A publication Critical patent/CN110476744A/en
Priority to PCT/CN2020/114086 priority patent/WO2021052222A1/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 

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  • Life Sciences & Earth Sciences (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention belongs to brassinosteroid biosynthesis technical fields, disclose disease-resistant anti-fall flower leaf type rape and are increasing the application on rape group child's hair twisted in a knot-childhood pericarp area.Applicant has found that leaf area index can be increased using disease-resistant anti-fall flower leaf type rape dense planting for the first time, to increase the effective photosynthetic capacity of seedling stage group, is converted in the maturity period and increases group's child's hair twisted in a knot-childhood pericarp area, to increase the application under dense planting on yield of rape.Child's hair twisted in a knot-childhood pericarp area and total yield have significant amplification, respectively 27.1%, 23.2% and 23.0%, 31.1% to floral leaf material 74277 and 74296 of the two of them with disease-resistant anti-fall characteristic (under especially line number increases to 1 meter of 6 row or 7 rows) under highly dense.

Description

Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area
Technical field
The invention belongs to brassinosteroid biosynthesis technical fields, and in particular to disease-resistant anti-fall flower leaf type rape is increasing rape unit Application on area child's hair twisted in a knot-childhood pericarp area and yield.
Background technique
Rape is the most important oil crops in China, and domestic rape oil accounts for 50% or more of domestic oil crops oil production.But With the development of economy, the cost inputs day such as the labour in crop planting and relevant cultivation management aobvious increase, and it is current Rape per unit area yield is relatively low, and productivity effect is relatively low, has seriously affected the enthusiasm of peasant planting rape and the confession of national edible oil To safety.
The crops such as monocotyledonous rice, wheat using dwarf character by making planting density, lodging resistance and fertilizer tolerability aobvious It writes and improves, considerably increase the effective photosynthetic capacity of leaf area index and group to increase considerably per unit area yield and facilitate first The secondary green revolution.However for a long time, natural selection and evolution make the dicotyledonous crops such as rape, soybean form seedling stage plant raw Object amount is big, the open and flat dispersion of blade, the features such as lower layer closing illumination is insufficient and maturity period branch and silique are more dispersed, these Characteristic promote the dicotyledons such as rape, soybean planting density and harvest index can not, and group's efficiency of light energy utilization is low, not catch up with The upper first time green revolution, rape harvest index is still one of minimum in all large crops at present, is also far from playing it Due yield potential.
Yield of rape is improved deeply to be ground from the high-yield culturings such as fertilizer factor, cultivation technique, rational close planting angle Study carefully and applies.But in breeding angle, breeders pay attention to single character, the parsing of the Regulation Mechanism of individual gene, ignore group's strain Type index study.Rational close planting is the key that make full use of the natural resources such as arable land and water, fertilizer, air and heat to win high crop yield to arrange One of apply.Rape will obtain high yield, must also adhere to rational close planting.Blade profile is the basis that crop plant type is formed, according to the property of leaf margin The blade profile of shape, cabbage type rape can be generally divided into three classes: roundleaf type, osculant (half floral leaf) and flower leaf type, wherein split quarter base It is known as floral leaf (Liu Houli, 2000) because causing leaf margin to show serration form.At present using mostly roundleaf and half flower in production Leaf rape variety is roundleaf and half floral leaf rape variety unit area within the scope of 150,000 plant of -75 ten thousand plant/hectare in planting density Yield first increases and subtracts afterwards, and yield optimum density is in 300,000-60 ten thousand plants/hectare, for the most roundleaves promoted in current production And half floral leaf rape variety, increase planting density, yield, which has no, to be obviously increased.Under existing leaf structure, since seedling stage plants Competition for light between strain, effective rate of utilization is low, and yield, or even meeting can not be effectively increased by enhancing roundleaf and half floral leaf rapeseed cultivation density Cause the underproduction (Bai Guiping etc., 2015), also obtained in soybean similar conclusion (Buddha's warrior attendant, 2003;Lv Shucai, 2018).It is main Reason is that dense planting can increase leaf area index to a certain extent, but relative sunshine intensity presentation decline becomes in its canopy Gesture, the effective photosynthetic capacity decline of group, the decline of yield when eventually leading to highly dense.
The present invention has chosen 2 kinds of roundleaf materials and 3 kinds of floral leaf materials, is provided with totally 18 plantations of 3 groups of strain numbers and 6 groups of line numbers Density, experiment results proved have the floral leaf material of disease-resistant anti-fall characteristic in high density, can dramatically increase rape leaf area and refer to Several and the total silique number of unit area group and child's hair twisted in a knot-childhood pericarp area, to increase yield, effect of increasing production reaches 31.1%.
Summary of the invention
The purpose of the present invention is to provide disease-resistant anti-fall flower leaf type rapes to increase the application in yield of rape.
It is another object of the present invention to provide disease-resistant anti-fall flower leaf type rapes to refer in increase seedling stage rape leaf area Application in number.
It is also an object of the present invention to provide disease-resistant anti-fall flower leaf type rapes to increase seedling stage rape population photosynthesis Application in ability, the total silique number of unit area group or child's hair twisted in a knot-childhood pericarp area.
In order to achieve the above object, the present invention takes lower technical solution to realize:
Disease-resistant anti-fall flower leaf type rape is increasing the application in yield of rape, utilizes disease-resistant anti-fall floral leaf including this Rape increases seedling stage rape leaf area index or increases seedling stage rape Photo distribution in population, or increases unit area group Total silique number, or increase child's hair twisted in a knot-childhood pericarp area, to achieve the purpose that volume increase;
In above-described application, it is preferred that the rape is cabbage type rape;
In above-described application, it is preferred that disease-resistant anti-fall character in the disease-resistant anti-fall flower leaf type rape with In double No. 9 under identical planting environment there was no significant difference or in being better than it is No. 9 double;
In above-described application, it is preferred that the method for plantation is every meter of 5-7 row;
In above-described application, it is preferred that the method for plantation is every meter of 5-7 row, every row 25-50 plants;
In above-described application, it is preferred that the disease-resistant anti-fall type floral leaf rape be rape floral leaf material 74277, 74296。
Compared with prior art, the invention has the following advantages that
The present invention finally found that by the exploration of screening and planting patterns to rape variety, disease-resistant anti-fall flower leaf type Rape can increase yield of rape, and the increase of the yield is by increasing seedling stage rape leaf area index or increasing seedling stage rape Photo distribution in population, or increase the total silique number of unit area group, or increase child's hair twisted in a knot-childhood pericarp area, to reach volume increase Purpose, effect of increasing production reach 31.1%.
Detailed description of the invention
Fig. 1 is 3 floral leaves and 2 roundleaf material shapes.
Fig. 2 is that field trial is taken photo by plane figure.
Fig. 3 is labeled leaf chlorophyll content downward trend schematic diagram under different densities.
Fig. 4 is rape colonial organism amount and leaf area index variation tendency schematic diagram under different densities.
Fig. 5 is that single plant yield and its correlated traits identify schematic diagram under different densities.
Fig. 6 is that the lower 5 material population yields of different densities and its correlated traits identify schematic diagram.
Specific embodiment
Floral leaf material 74277,74295,74296 used in the present invention, roundleaf material 74265,74273 are purchased from business Channel.Rape used in the embodiment of the present invention is cabbage type rape.
Embodiment 1:
Rape floral leaf material 74277,74295,74296, the character observation of rape roundleaf material 74265,74273:
Rape floral leaf material 74277,74295,74296, rape roundleaf material 74265,74273 are purchased from Wuhan oily Zhong Ye Science and Technology Ltd..By above-mentioned material in September, 2017 with low-density (1 meter of 3 row, compartment is 2 meters wide, 10 plants of every row), sowing In Inst. of Oil Crops, Chinese Academy of Agriculture's Wuchang Experimental Base, 3 repetitions of experimental setup.To above-mentioned after sowing 60 days Material blade profile, light transmittance are identified, are investigated the number of blade, biomass within 90 days in sowing, flowering period are recorded, in maturation Phase investigates part plant type and Correlated Yield Characters (plant height, branch height, branch amount, silique number, stalk thick etc.).Specifically It is as follows:
13 floral leaves of table and 2 roundleaf material transmissivities, leaf characters and flowering time
23 floral leaves of table and 2 roundleaf material part plant types and yield traits
Embodiment 2:
The identification of the anti-sclerotiniose and lodging resistance of floral leaf and roundleaf material
Double No. 9 using in disease-resistant anti-fall normal rapeseed kind as control, by above-mentioned material and in pairs No. 9 in September, 2018 In oil plant research institute experimental field, test carries out RANDOMIZED BLOCK DESIGN for bottom plantation, and each cell plants 10 rows, according to 1 meter of 3 row, often 20 plants of row, in triplicate, field management is identical as general crop field.Carry out property determination when, in distance limit 30cm in cell with It inside randomly selects 15 plants to be measured, and takes its average value as character value.
The method that Yang Xiangdong (2006) are participated in anti-sclerotiniose identification carries out field mirror using toothpick method in the rape whole florescence It is fixed.Toothpick is boiled into 15min in boiling water, is put into PDA culture medium, then high pressure sterilization is arranged radially solid in PDA In body culture medium, the nuclear fungal hyphae block that diameter is 8mm is placed in ware center.23 DEG C culture 3-5 days after to cover with bacterium on toothpick Silk.Then toothpick is seeded on the rape stem away from ground 30cm, measures scab length (cm) after a week.Each cell measurement 15 plants, calculate average value.
The method that Yang Xiangdong (2006) are participated in lodging resistance identification is measured during rape is mature with straw stiffness analyzer Rape stem intensity.It chooses and grows normal rape, clip upper layer canopy part in stem center, it is with stalk strong instrument that plant is curved Have a certain degree (45 °), measures strength of stem (N/ stem).15 plants of each cell measurement calculates average value.
33 floral leaves of table and the anti-sclerotiniose of 2 roundleaf materials and lodging resistance measurement
Blade profile Material number Middle part stalk is thick (mm) Scab length Strength of stem (N/ stem) Lodging degree (area) %
Roundleaf 74265 11.3 5.06a 107.64 0%
Roundleaf 74274 12.5 4.61 131.37a 0%
Floral leaf 74277 13.3 4.77 120.55 0%
Floral leaf 74295 11.9 6.18a 87.63a 5%a
Floral leaf 74296 11.5 5.03 109.84 0%
Control In double No. 9 12.3 4.84 113.45 0%
Note: letter a refers to compared with No. 9 double in control after data, in 0.05 horizontal upper significant difference
Data are shown in table 3, double in floral leaf material 74277,74296 and roundleaf material 74265,74274, with check variety No. 9 are compared, and in middle part stalk, slightly, in resistance to sclerotinia sclerotiorum, lodging degree there is no significant differences;Floral leaf material 74277,74296 With roundleaf material 74265 on straw stiffness, with double No. 9 in check variety compared to there is no significant difference, and roundleaf material 74274 straw stiffness be significantly higher than in double No. 9;Another floral leaf material 74295, in scab length, straw stiffness and lodging In degree, there is significant difference compared with No. 9 double in check variety, illustrate that the disease resistance of floral leaf material 74295, lodging resistance are weak Yu Zhongshuan 9.
Embodiment 3:
The floral leaf of cabbage type rape is increasing the application on seedling stage Photo distribution in population and group's child's hair twisted in a knot-childhood pericarp area:
3 floral leaves and 2 roundleaf rape different densities split plot experiment designs
The present embodiment investigates 3 floral leaf materials, 74277,74295,74296 and 2 roundleaf materials 74265,74273 more It is planted under group line number and strain number combined density, for identifying that yield of the rape difference blade profile material in high-density planting becomes Change, and causes the factor of change of production.Split block design is taken in test, and 3 repetitions are arranged.Below in compartment wide 1.8 meters divide into It sets:
Principal element: line number, including 1 meter of 4 row, 1 meter of 5 row, 1 meter of 6 row, 1 meter of 7 row, 1 meter of 8 row, 1 meter of 9 row;
Secondary cause: strain number, including 30 plants/row, 40 plants/row, 50 plants/row;
Split plot again: 3 floral leaf materials and 2 roundleaf materials.
It altogether include 18 planting densities, 240 cells.
It tests and was seeded in Inst. of Oil Crops, Chinese Academy of Agriculture's Wuhan Yangluo planting matrix on September 26th, 2018 Ground, each cell cultivated area are 5.4m2, first time thinning is carried out according to planting density within after planting the 10th day, carry out within the 18th day Second of thinning, progress final singling in the 25th day.Rape seedling growth and development and maturity period population characteristics will to the response of planting density Later period yield composition is directly affected, the present invention reflects to different blade profile materials in growth of rape early stage and maturity period characters with plant It is fixed, the response to clear rape difference blade profile to high-density planting.
At after planting the 30th day, the consistent rape of 7 plants of growing ways is randomly choosed in the 4th row of each cell, demarcates its third piece Young leaves.The 6th day after index blade, the 12nd day, the 18th day, the 24th day, calibration leaf is measured using SPAD-502 and third piece is new The chlorophyll content of functional leaf, by chlorophyll content ratio (labeled leaf/third piece new function leaf), under Lai Fanying different densities The lower blade of different blade profile materials with caused by vine growth and development chlorophyll reduce, yellow leaf phenomena such as and its As the time of consumption organ, to judge the influence of blade profile and planting density to leaf senile.
Rape seedling growing way influences substance accumulation and overwintering rear developmental potency before the rape winter, and different growth potential rapes are not It is different with being showed under density cultivation condition.Influence for clear different densities to above-mentioned 5 Material growth gesture, the present invention are right first After planting 50 days (seedling growth stage), after planting 120 days (first a kind of sedge phase) two time points, the leaf area of different materials under each density Index is investigated, leaf area/land area on leaf area index (LAI)=unit soil.In addition to leaf area index, this hair It is bright that also after planting 50 days, 80 days, 120 days rape colonial organism amounts are investigated, to reflect different densities to above-mentioned 5 materials The influence of growth potential.
In crop yield forming process, each Characters on Yield formation contribution is different, and interact size between each character Also variant.Rape population yield depends on unit area single plant number and single plant yield, therefore the present invention is in the maturity period, right 240 cells the 8th, 9,10, all single plants of totally 4 rows (including sclerotiniose infection lodging single plant) the progress species test of 11 rows, before species test, This 4 row lodging single plant number is investigated first, species test character includes every row single plant number, single plant dry matter weight, plant height, branch Highly, the characters such as the effective silique number of main sequence height, branch amount, single plant, silique surface product, mass of 1000 kernel, single plant yield, and take all Single plant average value is as character value.
Embodiment 4:
The correlation of different blade profile rape seedlings and maturity period character and yield
Density to rape individual and astogeny be affected, understand different densities under rape seedling and maturity period character and The correlation of itself and single plant yield and population yield can analyze dense planting different blade profile rapes shadow caused by from seedling stage to the maturity period The reason of ringing, and its population yield caused to change.
By the way that above-mentioned 5 materials, seedling stage and maturity period Characters Identification, the present embodiment are analyzed under 18 planting densities Same material is under different densities, the correlation of single plant yield and population yield and seedling stage and maturity period investigation character.
Table 4 74265 is related between Seedling Characters and maturity period character and single plant yield, population yield under different densities Property
Single plant yield is mainly investigated always with single plant silique number, branch amount, biomass, third time under the conditions of table 4 shows dense planting Leaf/young leaves chlorophyll ratio (i.e. leaf senile degree) is positively correlated in significant, but it is it is similarly subjected to the influence of canopy index, It lodges with number of plants per unit area (i.e. planting density), early stage leaf-area coefficient, early stage the upperground part biomass and maturity period rape Degree is in significant negatively correlated.Illustrate that population density increase will lead to single plant yield and reduce, and seedling stage brassinosteroid biosynthesis under the conditions of dense planting Amount increases and leaf-area coefficient increasing degree, blade anti-aging rate influence later period single plant yield and formed, and the anti-sclerotiniose of material is anti- Lodging performance also influences single plant yield and is formed.
And population yield depends on single plant yield and number of plants per unit area, but in population characteristics, population yield and maturation Phase unit area silique number, unit area silique surface product are positively correlated in significant, with group's lodging degree in significant negatively correlated, Middle unit area silique surface product is up to 0.935 with population yield correlation.
Population yield depends primarily on unit area silique surface product increasing degree under the conditions of above data illustrates dense planting, and It is anti-including seedling stage and maturity period Characters of Plant Type and material whether the response of single plant character pair density determines population yield increase The anti-fall characteristic of disease.
Embodiment 5: vegetative growth phase blade profile and planting density shift to an earlier date the influence of aging to lower blade
Correlation analysis shows that third time investigation labeled leaf/young leaves chlorophyll ratio is significant related to single plant yield, because This, the present invention carries out detailed analysis to reaction of the labeled leaf/young leaves chlorophyll ratio under different densities.
Fig. 3 shows: under all planting densities, all material for the first time and in second of investigation, (mark by chlorophyll ratio Remember leaf/third piece new function leaf) between 0.772-1.076, as density increases, same material chlorophyll ratio difference compared with It is small or without significant difference, illustrate that labeled leaf as young leaves, has normal photosynthetic capacity.And in third time investigation, two parts The chlorophyll ratio of roundleaf material 74265 (0.465-0.761) and 74273 (0.302-0.861) with plantation line number increase, In significant downward trend, downward trend is significantly greater than three parts of floral leaf materials 74277 (0.605-1.058), 74295 (0.660- And 74296 (0.539-1.005) 1.012).Three parts of floral leaf materials under 30 plants, 1 meter of 4 row to 1 meter of 6 line density, investigate by third time Chlorophyll ratio compared with the first and second survey datas, no significant difference, only chlorophyll ratio is significant under more high density Decline.
Fig. 3 is also shown, third time investigate when, it is either highly dense or it is low it is close under the conditions of, the leaf of two parts of roundleaf materials is green Significantly less than three parts floral leaf materials (P < 0.05) of plain ratio.For example: under 30 plants, the planting density of 1 meter of 7 row, two parts of circles Leaf material chlorophyll ratio is 0.602 and 0.608, and the chlorophyll ratio of floral leaf material is then 0.886,0.867,0.770.In When the 4th is investigated, part roundleaf material marking blade falls off because of jaundice aging.
The above results explanation, under the conditions of highly dense, floral leaf and roundleaf blade profile are diligent to seedling leaf enforcement normal light cooperation The effective time of energy, which has, to be significantly affected.Under the conditions of dense planting, blade is interlaced, and roundleaf lower blade will turn yellow in advance, decline Always, ingredient consumes organ, influences plant entirety photosynthesis, this builds up high degree influence plant type of upper later period, and floral leaf can Influence caused by shifting to an earlier date aging blade as dense planting is effectively reduced.
Embodiment 6: the response of colonial organism amount and leaf area index to planting density
Leaf area index (LAI) be measure crop groups canopy leaves size and influence Photo distribution in population it is important because Element, and biomass then reflects group's growing way.Fig. 4 is pointed out, when investigating first time, colonial organism amount increases substantially as line number And increase, only some materials biomass at highly dense (1 meter of 9 row) declines;When investigating for second, colonial organism amount exists substantially 1 meter of 7 row or 1 meter of 8 row reach peak value;And when the 3rd investigation, colonial organism amount reaches peak in 1 meter of 6 row or 1 meter of 7 row substantially Value, is decreased obviously under higher line number.When third time is investigated, material is in first a kind of sedge phase, which directly affects Later period plant type is built up, but wherein the maximum biomass of 3 materials (74265,74277,74295) is both present in 30 plants of conditions of every row Under, the increase of colonial organism amount mostlys come from the increase of line number under dense planting, rather than the increase of strain number.
The colonial organism amount of investigation is consistent with first time, and the LAI of investigation increases substantially as line number and increased for the first time, Only there are two materials 74265 and 74296 when line number increases to 1 meter of 8 row, and LAI reaches peak value, declines in 1 meter of 9 row.And When second (first a kind of sedge phase) investigation, under the conditions of different strain numbers, 5 materials are under high line number, and LAI goes out when especially 1 meter of 9 row Now drop, and first a kind of sedge phase leaf area index will directly affect later period plant type and build up, to influence yield.Illustrate that rational close planted can increase Oiling dish group LAI, but in more high density, LAI can decline instead as density increases.
Compared to 1 meter 4 row, 30 plants of initial density, in second crucial of investigation, floral leaf material 74277,74295, 74296 after density increase leaf area index highest amplification be respectively 88.9%, 59.0% and 139.5%, and roundleaf material 74263 and 74275 highest amplification is respectively 35.7% and 55.5%.In the case where increasing planting density, floral leaf material leaf area refers to Number, which increases, is noticeably greater than roundleaf material, and the rate of its functional leaf aging is significantly less than roundleaf material, mutually folds both herein In the case where adding, effective photosynthetic capacity of vegetative growth phase floral leaf material will be significantly higher than that roundleaf material, seedling stage population photosynthesis energy The enhancing of power is to improve the premise of later period photosynthetic yield accumulation.
Embodiment 7:
The response of single plant yield and its correlated traits to planting density
Based on above-mentioned correlation analysis as a result, the present invention under different densities influence single plant yield formed single plant character into Row analysis, Fig. 5 are the lower 5 material single plant yields of different densities, single plant branch amount and single plant silique number in 6 line numbers and 3 strains Several lower variation tendencies.
Fig. 5 and table 5 show, significant change has occurred with the increase of planting density in the single plant yields of this 5 materials, In, the single plant yield of roundleaf material 74265 and 74273 and a floral leaf material 74295 increases with line number, and yield presents obvious Downward trend declines most 43.8%-89.2%, 41.6%-84.4% and 30.2%-62.5% respectively.And floral leaf material 74277 and 74296 when line number increases to 5,6,7, single plant yield there is no with line number increase occur being remarkably decreased trend or Fall is significantly less than other three materials, and only under the conditions of high line number 8,9 row, single plant yield is remarkably decreased.Branch amount and Single plant silique number and single plant yield have similar variation tendency.
Above data explanation increases rape seed line number, the influence to part floral leaf material single plant yield to a certain extent Significantly less than the influence to roundleaf material, the variation of single plant yield has statistics to the response of planting density between floral leaf different cultivars Meaning is learned, will affect later period yield composition.
Table 5: the lower 5 materials single plant change of production ratio of different densities (with 1 meter of 4 row, 30 plants are compared)
Embodiment 8: the response of population yield and maturity period character pair planting density
The effect of dense planting most critical is that no increase population yield, the present invention investigate 240 cell productions, hair The maximum output of existing roundleaf material 74265 appears in 1 meter of 6 row, 30 plants of density, compared to 1 meter 4 row, 30 plants of initial density, Its amplification is 8.4%, but difference is not significant;The maximum output of roundleaf material 74273 appears in 1 meter of 4 row, 40 plants of density, phase Than in initial density, amplification 5.3%, in 1 meter of 6 row, 30 plants of density also has 4.6% amplification, but difference is not significant; The maximum output of floral leaf material 74277 appears in 1 meter of 6 row, 30 plants of density, amplification 23.0%, significant difference;Floral leaf material Material 74295, compared to initial density, there is amplification under 1 meter of 4 row, the increased situation of strain number in yield, but difference is not significant; The maximum output of floral leaf material 74296 appears in 1 meter of 7 row, 40 plants of density, amplification 31.1%, in 1 meter of 7 row, 30 plants Also occur larger amplification, amplification 25.6%, significant difference when density.
And according to phenotype correlation analysis, the present invention also analyzes the group's characters with plant for influencing population yield, The total silique number of middle unit unit area and child's hair twisted in a knot-childhood pericarp area and population yield have similar rule, increase to centainly in density When degree, mainly by the increase of total silique number and child's hair twisted in a knot-childhood pericarp area, to increase population yield.Two of them have The floral leaf material 74277 and 74296 of disease-resistant anti-fall characteristic child's hair twisted in a knot-childhood (under especially line number increases to 1 meter of 6 row or 7 rows) under highly dense Pericarp area has significant amplification, respectively 27.1%, 23.2%, and other two roundleaf materials 74265 and 74277, and Another disease-resistant lower floral leaf material 74295 of lodging resistance, child's hair twisted in a knot-childhood pericarp area do not have significant amplification.
Above data illustrates density increase to a certain extent, especially the increase of line number, has for above-mentioned 5 rape varieties There is certain positive result, but high density will lead to the material underproduction instead.Wherein density increases to two 74277 Hes of floral leaf material 74296 effect of increasing production be much larger than two roundleaf materials 74265 and 74273, but and not all floral leaf material have to density Higher effect of increasing production, dense planting have high requirement to the disease-resistant lodging resistance of floral leaf material.
In conclusion disease-resistant anti-fall flower leaf type rape can be by extending seedling leaf photosynthesis time and blade face under dense planting Product index, to increase seedling stage Photo distribution in population, so that increasing group's child's hair twisted in a knot-childhood pericarp area in the maturity period, to increase close Plant lower yield of rape.
Table 6: the group under (1 meter of 4 row, 30 plants be initial density) 3 floral leaves and 2 roundleaf different densities compared with initial density Body production ratio

Claims (8)

1. disease-resistant anti-fall flower leaf type rape is increasing the application on yield of rape.
2. disease-resistant anti-fall flower leaf type rape is increasing the application in the effective photosynthetic capacity of seedling stage group.
3. disease-resistant anti-fall flower leaf type rape is increasing the application on the total silique number of rape unit area.
4. disease-resistant anti-fall flower leaf type rape is increasing the application on rape unit area child's hair twisted in a knot-childhood pericarp area.
5. a kind of method for increasing rape unit area child's hair twisted in a knot-childhood pericarp area and yield, including by disease-resistant anti-fall flower leaf type rape It is planted with the density of every meter of 5-7 row.
6. any application in -5 according to claim 1, the rape are cabbage type rape.
It is disease-resistant anti-fall in the disease-resistant anti-fall flower leaf type rape 7. any application in -5 according to claim 1 Character in double No. 9 under identical planting environment there was no significant difference or in being better than it is No. 9 double.
8. according to the method described in claim 5, it is characterized in that, the disease-resistant anti-fall flower leaf type rape is rape floral leaf Material 74277 and 74296.
CN201910879142.2A 2019-09-18 2019-09-18 Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area Pending CN110476744A (en)

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CN201910879142.2A CN110476744A (en) 2019-09-18 2019-09-18 Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area
PCT/CN2020/114086 WO2021052222A1 (en) 2019-09-18 2020-09-08 Use of mosaic trait for increasing total pericarp area of oilseed rape population

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021052222A1 (en) * 2019-09-18 2021-03-25 中国农业科学院油料作物研究所 Use of mosaic trait for increasing total pericarp area of oilseed rape population
CN113652499A (en) * 2021-09-16 2021-11-16 中国农业科学院油料作物研究所 Molecular marker closely linked with rape selenium high-efficiency character major QTL locus qSe.C07 and application

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101336611B (en) * 2008-07-04 2010-12-29 江苏丘陵地区镇江农业科学研究所 Breeding method of good quality, double-low, disease-resistant, high yield, lodging-resistant rape
CN102845297A (en) * 2012-08-22 2013-01-02 江苏丘陵地区镇江农业科学研究所 Breeding method of rape variety suitable for machine harvesting and sowing
CN103493652A (en) * 2013-08-24 2014-01-08 铜陵市天河绿色生态农业有限公司 Organic double-low oilseed rape cultivation method
CN104041289A (en) * 2014-06-25 2014-09-17 青岛博洋生物技术有限公司 Rape planting method
CN104604556A (en) * 2015-01-22 2015-05-13 华中农业大学 Method for improving pod shatter resistance of silique during mature period of cabbage type rape variety and application thereof
CN104885746A (en) * 2015-05-18 2015-09-09 华中农业大学 Cultivation method capable of reducing mechanized harvesting loss rate of rape and application thereof
CN105115896A (en) * 2015-09-10 2015-12-02 陕西省杂交油菜研究中心 Method for determining photosynthetic characteristics of plant silique
CN106069071A (en) * 2016-06-25 2016-11-09 合肥隆扬农业科技有限公司 A kind of implantation methods of Brassica campestris L
CN109041875A (en) * 2018-06-19 2018-12-21 华中农业大学 The yield of rape based on economical character and the prediction technique of lodging resistance

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103931486B (en) * 2014-04-28 2015-12-30 上海市农业科学院 The application of cabbage type rape floral leaf mutant
CN104221849A (en) * 2014-09-26 2014-12-24 浙江省农业科学院 Breeding method for controlling purity of brassica napus recessive epistatic genic male sterile hybrids
WO2017031686A1 (en) * 2015-08-25 2017-03-02 罗明镜 Rape cultivation method
CN105993924B (en) * 2016-06-27 2018-04-17 湖南省作物研究所 A kind of application of the selection of the high yield of resistance to dense planting Brassica napus hybrid kind and obtained napus hybrid kind
CN110476744A (en) * 2019-09-18 2019-11-22 中国农业科学院油料作物研究所 Floral leaf character is increasing the application on rape group child's hair twisted in a knot-childhood pericarp area

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101336611B (en) * 2008-07-04 2010-12-29 江苏丘陵地区镇江农业科学研究所 Breeding method of good quality, double-low, disease-resistant, high yield, lodging-resistant rape
CN102845297A (en) * 2012-08-22 2013-01-02 江苏丘陵地区镇江农业科学研究所 Breeding method of rape variety suitable for machine harvesting and sowing
CN102845297B (en) * 2012-08-22 2014-11-26 江苏丘陵地区镇江农业科学研究所 Breeding method of rape variety suitable for machine harvesting and sowing
CN103493652A (en) * 2013-08-24 2014-01-08 铜陵市天河绿色生态农业有限公司 Organic double-low oilseed rape cultivation method
CN104041289A (en) * 2014-06-25 2014-09-17 青岛博洋生物技术有限公司 Rape planting method
CN104604556A (en) * 2015-01-22 2015-05-13 华中农业大学 Method for improving pod shatter resistance of silique during mature period of cabbage type rape variety and application thereof
CN104885746A (en) * 2015-05-18 2015-09-09 华中农业大学 Cultivation method capable of reducing mechanized harvesting loss rate of rape and application thereof
CN105115896A (en) * 2015-09-10 2015-12-02 陕西省杂交油菜研究中心 Method for determining photosynthetic characteristics of plant silique
CN106069071A (en) * 2016-06-25 2016-11-09 合肥隆扬农业科技有限公司 A kind of implantation methods of Brassica campestris L
CN109041875A (en) * 2018-06-19 2018-12-21 华中农业大学 The yield of rape based on economical character and the prediction technique of lodging resistance

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
刘唐兴等: "油菜倒伏指数和茎秆生化成分及农艺性状的灰色关联分析", 《中国油料作物学报》 *
张亚龙等: "《作物栽培技术》", 30 April 2015, 中国农业大学出版社 *
张耀文等: "油菜角果光合特性研究现状及改良思路", 《中国油料作物学报》 *
沈广等: "《中国农村科技致富实用全书》", 30 November 1993, 北京农业大学出版社 *
马霓等: "我国南方冬油菜机械化生产农艺研究进展", 《中国油料作物学报》 *
马霓等: "甘蓝型油菜抗倒伏性及农艺性状研究", 《作物杂志》 *
黄善香: "《中国种植养殖技术百科全书 第1卷》", 31 March 1999, 南方出版社 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021052222A1 (en) * 2019-09-18 2021-03-25 中国农业科学院油料作物研究所 Use of mosaic trait for increasing total pericarp area of oilseed rape population
CN113652499A (en) * 2021-09-16 2021-11-16 中国农业科学院油料作物研究所 Molecular marker closely linked with rape selenium high-efficiency character major QTL locus qSe.C07 and application
CN113652499B (en) * 2021-09-16 2023-06-09 中国农业科学院油料作物研究所 Molecular marker closely linked with rape selenium efficient property main effect QTL locus qSe.C07 and application

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