CN110449195A - A kind of device for improving drop and dividing the uniformity in asymmetry channel - Google Patents
A kind of device for improving drop and dividing the uniformity in asymmetry channel Download PDFInfo
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- CN110449195A CN110449195A CN201910838432.2A CN201910838432A CN110449195A CN 110449195 A CN110449195 A CN 110449195A CN 201910838432 A CN201910838432 A CN 201910838432A CN 110449195 A CN110449195 A CN 110449195A
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- drop
- channel
- bypass passage
- continuous phase
- main channel
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0887—Laminated structure
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/12—Specific details about materials
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
Abstract
A kind of device the invention discloses raising drop in the asymmetry channel division uniformity can control sub- droplet size after division by adjusting flow pump discharge.Belong to micro-fluidic chip application field.The micro-fluidic chip of asymmetry channel includes the cover plate and substrate made by PDMS.Discrete phase liquid and continuous phase liquid are passed through microchannel from entrance I and entrance II respectively using flow pump, two different liquid generate drop at T shape channel, keep that flow is constant that drop is made to reach stable generation state, stop being passed through for the side liquid of dispersed phase and continuous phase later, and the connection of dispersed phase and continuous phase entrance and flow pump is cut off, make channel interior full of drop of uniform size by this method.Later by being twitched to drop generated in channel using flow pump, to realize drop is more uniform in asymmetry channel division.
Description
Technical field
The present invention relates to a kind of raising Microfluidic droplets in the device of the asymmetry channel division uniformity, passes through adjusting flow
Pump discharge can control sub- droplet size after division.Belong to micro-fluidic chip application field.
Background technique
Micro-fluidic chip is to carry out sequence of operations to fluid on micron or nanoscale, to realize the new of specific control
Type technology.The technology is related to multiple basic subject fields such as mechanics, biology, chemistry, physics, and can be included by each subject
Basic function integrate onto one several square centimeters of chip.
Branch of the Microfluidic droplet technology as microflow control technique realizes the life of microlayer model by manipulation very low volume fluids
At, the control functions such as transport, divide, merge, sort, position and capture.In biological or chemical research, single drop or bubble
It can be used as chemical reaction, transport and mixture, and played in the industries such as food and medicine manufacture, particle preparation, protein crystal
Important function.Droplet size uniformity can generate very important influence to whole process, and people will more and more pay close attention to
The uniformity of microscale droplets in microfluidic system, it is a kind of simple and feasible side that droplet size is adjusted by making drop breakup
Method.
Drop breakup can accurately manipulate the uniformity for possessing the microlayer model of micro- reaction platform function, mature drop breakup
Technology has the advantages that promoting drop formation flux, realizing parallel reaction, improve reaction and detection efficiency.Due to microfluidic channel
Diversity and complexity, it is necessary to make asymmetry channel drop realize homolysis, thus realize give birth in several cases
At the relatively uniform sub- drop of size.
Summary of the invention
The present invention is based on the asymmetry channels for causing drop breakup, by using flow pump to drop already present in channel
It is twitched, improves the drop breakup uniformity in asymmetry channel to realize.Distinguish at two branch ends of asymmetry channel
It is inserted into one of plastic tube, the other end of plastic tube is connect with two syringes.Syringe is mounted on flow pump, flow is used
Pumping hydrodynamic drop, since the flow perseverance in two branches is equal, may be implemented the homolysis of asymmetry channel crotch drop.Knot
Fruit shows, under the same terms, about the uniformity of drop after division, drop breakup caused by twitching, which is substantially better than promotion, to be caused
Drop breakup.
The present invention improves the way of propelling for causing drop breakup in microchannel, and primary structure is as follows:
A kind of device for improving drop and dividing the uniformity in asymmetry channel, the device are the micro-fluidic core of asymmetry channel
Piece, including the cover plate 1 and substrate 2 made by PDMS, cover plate 1 and substrate 2, which match, is combined into the main structure of micro-fluidic chip, lid
The structure of piece 1 include discrete phase entrance 3, wing passage 7, continuous phase entrance 4, main channel 8, bypass passage I9, bypass passage II10,
I5, outlet II6 are exported, discrete phase entrance 3 is connect by the way that wing passage 7 is vertical with main channel 8, and the setting of continuous phase entrance 4 is main logical
The side in road 8, outlet II6 pass through bypass passage I9 and the oblique connection in main channel 8, and outlet I5 is logical by bypass passage II10 and master
Road 8 connects, and the other side of main channel 8 is arranged in outlet I5.
The region that fluid when each structure on cover plate 1 constitutes micro-fluidic chip work flows.8 one end of main channel and side
Channel 7 forms T-type channel, and the other end of main channel 8 is bypass passage I9 and bypass passage II10, and bypass passage I9 and branch are logical
Road II10 is connected by plastic tube with syringe 11, and syringe 11 is mounted on flow pump 12.
Conventional drop mitotic apparatus is that flow pump pushes dispersed phase and continuous phase to cause to divide, the droplet break-up device
Generated drop is twitched using flow pump to cause to divide, and the sub- drop generated after division can be made more uniform.Drop breakup
The uniformity of sub- droplet size afterwards by bypass passage I9 and bypass passage II10 flow effect, in order to allow bypass passage I9 and
The flow-rate ratio of bypass passage II10 is twitched using the same flow pump 12 close to identical.
The specific work process of the present apparatus is as follows: discrete phase liquid is flowed into from discrete phase entrance 3, and continuous phase liquid is from continuous
Phase entrance 4 flows into, and discrete phase liquid and continuous phase liquid cross in the T-type channel that wing passage 7 and main channel 8 are formed, discrete phase
Liquid forms drop under the action of continuous phase liquid, and keeps flow to make drop for a period of time toward downstream flow with continuous phase
Generation it is in stable condition, when a drop reach 8 downstream of main channel bifurcation mouth (i.e. the friendship fork of main channel 8 and bypass passage I9)
When, stable drop is filled in main channel 8, stops being passed through for the side liquid of dispersed phase and continuous phase at this time, and is cut off point
The connection of dephasing and continuous phase entrance and flow pump reuses flow pump 12 and twitches in bypass passage I9 and bypass passage II10
Liquid keeps the flow in two bypass passages equal.When drop is divided, under the influence of same traffic, two sides are identical
Sticky shearing stress can make the sub- drop after division close to equal.
The present invention generates the sub- drop that size is more nearly, related step after asymmetry channel drop breakup may be implemented
Rapid method is mature, the available guarantee of reliability.
Detailed description of the invention
Fig. 1 is that the present invention improves Microfluidic droplet in the three-dimensional of the micro-fluidic chip of asymmetry channel division uniformity device
Outline drawing.
Fig. 2 is that the present invention improves Microfluidic droplet in the course of work schematic diagram of asymmetry channel division uniformity device.
In figure: 1, cover plate, 2, substrate, 3, discrete phase entrance, 4, continuous phase entrance, 5, outlet I, 6, outlet II, 7, side it is logical
Road, 8, main channel, 9, bypass passage I, 10, bypass passage II, 11, syringe, 12, flow pump
Note:
Since microchannel size is much smaller than chip size, indicate clearly characterize when micro-fluidic chip with actual size micro-
The structure of fluidic chip runner section, therefore attached drawing uses the chip schematic diagram of the opposite amplification of micro-channel structure.
Specific embodiment
Below with reference to Structure Figure, Microfluidic droplet is improved in the work of the device of the asymmetry channel division uniformity to realizing
Make process and function and effect are described in detail.
The specific work process of the present apparatus is as follows: leading to discrete phase and continuous phase liquid, discrete phase liquid respectively using flow pump
Body is flowed into from discrete phase entrance 3, and continuous phase liquid is flowed into from continuous phase entrance 4, and discrete phase liquid and continuous phase liquid are logical in side
The T-type channel that road 7 and main channel 8 are formed crosses, and discrete phase liquid forms drop under the action of continuous phase liquid, and with continuous
Mutually toward downstream flow, flow is kept to make the generation of drop in stable condition for a period of time, made full of stable drop in channel, at this time
Stop being passed through for the side liquid of dispersed phase and continuous phase, and cuts off the connection of dispersed phase and continuous phase entrance and flow pump, then
The liquid in bypass passage I9 and bypass passage II10 is twitched using flow pump 12, keeps the flow in two bypass passages equal.When
When drop is divided, under the influence of same traffic, the identical sticky shearing stress in two sides can be such that the sub- drop after division connects
It is close equal.
Fig. 1 is that the present invention realizes raising Microfluidic droplet in the micro-fluidic chip of the device of the asymmetry channel division uniformity
Three-D profile schematic diagram.The chip includes cover plate 1, substrate 2, discrete phase entrance 3, wing passage 7, continuous phase entrance 4, main channel
8, bypass passage I9, bypass passage II10, outlet I5, outlet II6.Angle between bypass passage I9 and bypass passage II10 is
30 °, Fig. 2 is that the present invention realizes Microfluidic droplet in the course of work schematic diagram of the device of asymmetry channel homolysis.Two kinds
Liquid is flowed into micro-fluidic chip under the promotion of two flow pumps by two entrances, is adjusted the flow of two kinds of liquid, is made its life
At different size of microlayer model, and keeping flow for a period of time keeps the generation of drop in stable condition, is full of the feelings in channel in drop
Under condition, it is logical to reuse dynamic two branches of same traffic pumping for the connection of cutting and driving dispersed phase and the flow pump of continuous phase liquid
Road, the drop having been filled in channel issue estranged split in flow pump twitch.
Claims (4)
1. a kind of drop that improves is in the device of the asymmetry channel division uniformity, it is characterised in that: the device is asymmetry channel
Micro-fluidic chip, including the cover plate (1) and substrate (2) made by PDMS, cover plate (1) and substrate (2) matching be combined into miniflow
The main structure of chip is controlled, the structure of cover plate (1) includes discrete phase entrance (3), wing passage (7), continuous phase entrance (4), leads and lead to
Road (8), bypass passage I (9), bypass passage II (10), outlet I (5), outlet II (6), discrete phase entrance (3) pass through wing passage
(7) vertical with main channel (8) to connect, it is logical by branch to export II (6) in the side of main channel (8) for continuous phase entrance (4) setting
Road I (9) and main channel (8) oblique connection, outlet I (5) are connect by bypass passage II (10) with main channel (8), are exported I (5)
The other side in main channel (8) is set.
2. a kind of drop that improves according to claim 1 is in the device of the asymmetry channel division uniformity, it is characterised in that:
The region that fluid when each structure on cover plate (1) constitutes micro-fluidic chip work flows；Main channel (8) one end and wing passage
(7) form T-type channel, the other end of main channel (8) is bypass passage I (9) and bypass passage II (10), bypass passage I (9) and
Bypass passage II (10) is connected by plastic tube with syringe (11), and syringe (11) is mounted on flow pump (12).
3. a kind of drop that improves according to claim 2 is in the device of the asymmetry channel division uniformity, it is characterised in that:
The uniformity of sub- droplet size after drop breakup by bypass passage I (9) and bypass passage II (10) flow effect, in order to allow
The flow-rate ratio of bypass passage I (9) and bypass passage II (10) are twitched using the same flow pump (12) close to identical.
4. a kind of drop that improves according to claim 2 is in the device of the asymmetry channel division uniformity, it is characterised in that:
The specific work process of the present apparatus is as follows: discrete phase liquid is flowed into from discrete phase entrance (3), and continuous phase liquid is from continuous phase entrance
(4) it flows into, discrete phase liquid and continuous phase liquid cross in the T-type channel that wing passage (7) and main channel (8) are formed, discrete phase
Liquid forms drop under the action of continuous phase liquid, and keeps flow to make drop for a period of time toward downstream flow with continuous phase
Generation it is in stable condition, when a drop reach main channel (8) downstream bifurcation mouth, that is, main channel (8) and bypass passage I (9) friendship
When fork, it is filled with stable drop in main channel (8), stops being passed through for the side liquid of dispersed phase and continuous phase at this time, and
The connection for cutting off dispersed phase and continuous phase entrance and flow pump, reuses flow pump (12) twitch bypass passage I (9) and branch is logical
Liquid in road II (10), keeps the flow in two bypass passages equal；When drop is divided, in the influence of same traffic
Under, the identical sticky shearing stress in two sides can make the sub- drop after division close to equal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910838432.2A CN110449195A (en) | 2019-09-05 | 2019-09-05 | A kind of device for improving drop and dividing the uniformity in asymmetry channel |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910838432.2A CN110449195A (en) | 2019-09-05 | 2019-09-05 | A kind of device for improving drop and dividing the uniformity in asymmetry channel |
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| Publication Number | Publication Date |
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| CN110449195A true CN110449195A (en) | 2019-11-15 |
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| CN201910838432.2A Pending CN110449195A (en) | 2019-09-05 | 2019-09-05 | A kind of device for improving drop and dividing the uniformity in asymmetry channel |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113680402A (en) * | 2021-03-08 | 2021-11-23 | 王奔 | Single-reaction high-throughput microfluidic component, automatic nucleic acid amplification POCT system and droplet generation method |
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| JP2006263694A (en) * | 2005-03-22 | 2006-10-05 | Minoru Seki | Liquid drop division structure and method |
| US7918244B2 (en) * | 2005-05-02 | 2011-04-05 | Massachusetts Institute Of Technology | Microfluidic bubble logic devices |
| CN103347612A (en) * | 2011-02-07 | 2013-10-09 | 哈佛学院院长等 | System and method for splitting droplet |
| CN104801356A (en) * | 2015-04-14 | 2015-07-29 | 常州工学院 | Device and method for realizing splitting of micro-droplets in micro-channel under assistance of surface acoustic waves |
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2019
- 2019-09-05 CN CN201910838432.2A patent/CN110449195A/en active Pending
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| US5621443A (en) * | 1993-09-23 | 1997-04-15 | Heidelberger Druckmaschinen Ag | Ink-jet device and method of operation thereof |
| WO2005021151A1 (en) * | 2003-08-27 | 2005-03-10 | President And Fellows Of Harvard College | Electronic control of fluidic species |
| JP2006263694A (en) * | 2005-03-22 | 2006-10-05 | Minoru Seki | Liquid drop division structure and method |
| US7918244B2 (en) * | 2005-05-02 | 2011-04-05 | Massachusetts Institute Of Technology | Microfluidic bubble logic devices |
| CN103347612A (en) * | 2011-02-07 | 2013-10-09 | 哈佛学院院长等 | System and method for splitting droplet |
| CN104801356A (en) * | 2015-04-14 | 2015-07-29 | 常州工学院 | Device and method for realizing splitting of micro-droplets in micro-channel under assistance of surface acoustic waves |
| CN109289951A (en) * | 2018-10-29 | 2019-02-01 | 深圳先进技术研究院 | Drop breakup micro-fluidic chip and application |
| CN109825426A (en) * | 2019-02-21 | 2019-05-31 | 中国科学院苏州生物医学工程技术研究所 | Integral type drop microfluidic chip structure and preparation method, micro-fluidic chip component |
Non-Patent Citations (2)
| Title |
|---|
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113680402A (en) * | 2021-03-08 | 2021-11-23 | 王奔 | Single-reaction high-throughput microfluidic component, automatic nucleic acid amplification POCT system and droplet generation method |
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