CN110352724A - A kind of overwintering Juncao new varieties method for culturing seedlings - Google Patents
A kind of overwintering Juncao new varieties method for culturing seedlings Download PDFInfo
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- CN110352724A CN110352724A CN201910753485.4A CN201910753485A CN110352724A CN 110352724 A CN110352724 A CN 110352724A CN 201910753485 A CN201910753485 A CN 201910753485A CN 110352724 A CN110352724 A CN 110352724A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
The invention discloses a kind of overwintering Juncao new varieties method for culturing seedlings, the plant that the good Juncao of selection growth conditions and grass family can be overwintering first, and choose two kinds of healthy plant roots are washed with water and are completely carried out disinfection；2~3cm wedge shape is cut in Juncao root, cutting depth in the root that grass family is capable of overwintering plant is 3~4cm graft union, then grafts Juncao and is capable of the root of overwintering plant in grass family, then ties up fixation；The obtained plant grafted is placed in the environment of temperature is 30~40 DEG C and grows a period of time, ambient humidity is controlled 60~80%；The blade that selection healthy plant is newly grown carries out tissue cultures, obtains corresponding tissue-cultured seedling first filial generation；Method like this obtains corresponding four generation of tissue culture young plant, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties, the present invention can be realized Juncao in the perennation of northern area.
Description
Technical field
The invention belongs to Juncaos in northern area promotional technique field, and in particular to a kind of overwintering Juncao new varieties nursery side
Method.
Background technique
Juncao is to introduce China in nineteen eighty-three by national Juncao Engineering Technical Research Centre, is cultivated by more than 20 years suitable
The grass seeds of China's weather soil environment.Juncao is the upright bunch type plant of perennial grass family, has stronger tillering ability.This
To be a kind of be suitable in the torrid zone, the high yield and high quality fungus grass of subtropical zone growth and artificial cultivation.Healthy plant is tall and big, and resistance is strong, produces
Amount is high, and crude protein and sugar content are high.Every per mu yield fresh grass can be up to 30 tons or more.In addition to the production for being widely used in edible mushroom,
Also it can be used as high quality forage, while rhizome is very flourishing, it is criss-cross on regrowth plot, it can check winds and fix drifting sand, have good
Promotional value.Juncao south be it is perennial, but temperature be lower than 5 DEG C when Juncao can stop growing, be lower than 0 DEG C when can frostbite evil.
Prolonged frostbite can damage the root system of Juncao, its death is prevented to be introduced into behind the north increase from overwintering due to weather
The cost of plantation, also affects related industry in the development of northern area.
Summary of the invention
The object of the present invention is to provide a kind of overwintering Juncao new varieties method for culturing seedlings, can be realized Juncao in northern area
Perennation.
The technical scheme adopted by the invention is that a kind of overwintering Juncao new varieties method for culturing seedlings, specifically according to the following steps
Implement:
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of health
Plant root, which washes with water, completely to carry out disinfection；
Step 2 cuts 2~3cm wedge shape in Juncao root, and cutting depth in the root that grass family is capable of overwintering plant is 3
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by~4cm graft union；
When step 3, the plant grafted for obtaining step 2 are placed in the environment of temperature is 30~40 DEG C one section of growth
Between, ambient humidity is controlled 60~80%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 30~40 DEG C and grows a period of time by a generation, ambient humidity control 60~
80%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
The root of gramineae plant and when transplanting in the environment of temperature is 30~40 DEG C, humidity is 60~80% one section of culture growth
Between；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, in the environment of by second filial tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80% at one section of culture growth
Between；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80% at one section of culture growth
Between；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
The features of the present invention also characterized in that
The plant that grass family can be overwintering in step 1 is Chinese pennisetum.
It is sterilized in step 1 specific as follows:
First then 10~30s of plant root selected by 70% alcohol soaking step 1 is used with aseptic water washing 2~3 times
2% liquor natrii hypochloritis impregnates 10~15min or impregnates 5~10min with 0.1% mercuric chloride solution, and whole process constantly stirs,
It comes into full contact with plant root with 2% liquor natrii hypochloritis, 0.1% mercuric chloride solution, finally uses aseptic water washing 3~5 times.
The plant grafted in step 3 growth time control in the environment of temperature is 30~40 DEG C is 25~35 days.
Growth time control in the environment of temperature is 30~40 DEG C of tissue-cultured seedling first filial generation is 25~35 days in step 4.
It is 25~35 days that growth time, which controls, in step 5~step 10.
The invention has the advantages that a kind of overwintering Juncao new varieties method for culturing seedlings, has turned out bacterium with simple method
The overwintering new varieties of grass, solve the problems, such as that Juncao can not be overwintering in northern area, reduce the cost of plantation Juncao, and then make bacterium
Careless related industry obtains long term growth in northern area.
Specific embodiment
The present invention is described in detail With reference to embodiment.
A kind of overwintering Juncao new varieties method for culturing seedlings of the present invention, is specifically implemented according to the following steps:
(1) plant that step 1, the good Juncao of selection growth conditions and grass family can be overwintering, and by choose two kinds
Healthy plant root, which washes with water, completely to carry out disinfection, wherein the plant that grass family can be overwintering is Chinese pennisetum, and selection can be got over
The reason of winter gramineae plant is the survival rate of plant after grafting on the one hand can be improved, and it is overwintering on the other hand can to obtain its
Characteristic sterilizes in step 1 specific as follows:
First then 10~30s of plant root selected by 70% alcohol soaking step 1 is used with aseptic water washing 2~3 times
2% liquor natrii hypochloritis impregnates 10~15min or impregnates 5~10min with 0.1% mercuric chloride solution, and whole process constantly stirs,
It comes into full contact with plant root with 2% liquor natrii hypochloritis, 0.1% mercuric chloride solution, finally uses aseptic water washing 3~5 times.
Step 2 cuts 2~3cm wedge shape in Juncao root, and cutting depth in the root that grass family is capable of overwintering plant is 3
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by~4cm graft union；
When step 3, the plant grafted for obtaining step 2 are placed in the environment of temperature is 30~40 DEG C one section of growth
Between, growth time control is 25~35 days, and ambient humidity is controlled 60~80%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 30~40 DEG C and grows a period of time by a generation, growth time control for 25~
35 days, ambient humidity was controlled 60~80%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
The root of gramineae plant and when transplanting in the environment of temperature is 30~40 DEG C, humidity is 60~80% one section of culture growth
Between；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, in the environment of by second filial tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80% at one section of culture growth
Between；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80% at one section of culture growth
Between；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
It is 25~35 days that growth time, which controls, in step 5~step 10.
Embodiment 1
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of health
Plant root, which washes with water, completely to carry out disinfection, wherein the plant that grass family can be overwintering is Chinese pennisetum, sterilizes tool in step 1
Body is as follows:
Then the first plant root 10s selected by 70% alcohol soaking step 1 uses 2% chlorine with aseptic water washing 2 times
Acid sodium solution impregnates 15min or impregnates 5min with 0.1% mercuric chloride solution, and whole process constantly stirs, and makes plant root and 2%
0.1% mercuric chloride solution of liquor natrii hypochloritis comes into full contact with, and finally uses aseptic water washing 3 times.
Step 2 cuts 3cm wedge shape in Juncao root, cuts depth in the root that grass family is capable of overwintering plant and transfers for 4cm
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by interface；
Step 3, the plant grafted for obtaining step 2, which are placed in the environment of temperature is 30 DEG C, grows a period of time, raw
Control for a long time is 35 days, and ambient humidity is controlled 60%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 40 DEG C and grows a period of time by a generation, and growth time control is 25 days, environment
Humid control is 80%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
Simultaneously is transplanted in the environment of temperature is 30 DEG C, humidity is 60% culture growth a period of time in the root of gramineae plant；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, culture growth a period of time in the environment of by second filial tissue culture transplantation of seedlings to temperature be 40 DEG C, humidity is 60%；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 40 DEG C, humidity is 60% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, culture growth a period of time in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 40 DEG C, humidity is 80%；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 40 DEG C, humidity is 60% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
It is 35 days that growth time, which controls, in step 5~step 10.
Embodiment 2
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of health
Plant root, which washes with water, completely to carry out disinfection, wherein the plant that grass family can be overwintering is Chinese pennisetum, sterilizes tool in step 1
Body is as follows:
Then the first plant root 30s selected by 70% alcohol soaking step 1 uses 2% chlorine with aseptic water washing 3 times
Acid sodium solution impregnates 10min or impregnates 10min with 0.1% mercuric chloride solution, and whole process constantly stirs, make plant root and
2% liquor natrii hypochloritis, 0.1% mercuric chloride solution comes into full contact with, and finally uses aseptic water washing 5 times.
Step 2 cuts 2cm wedge shape in Juncao root, cuts depth in the root that grass family is capable of overwintering plant and transfers for 3cm
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by interface；
Step 3, the plant grafted for obtaining step 2, which are placed in the environment of temperature is 40 DEG C, grows a period of time, raw
Control for a long time is 25 days, and ambient humidity is controlled 80%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 30 DEG C and grows a period of time by a generation, and growth time control is 35 days, environment
Humid control is 60%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
Simultaneously is transplanted in the environment of temperature is 40 DEG C, humidity is 80% culture growth a period of time in the root of gramineae plant；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, culture growth a period of time in the environment of by second filial tissue culture transplantation of seedlings to temperature be 30 DEG C, humidity is 80%；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 30 DEG C, humidity is 80% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, culture growth a period of time in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 30 DEG C, humidity is 60%；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 30 DEG C, humidity is 80% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
It is 25 days that growth time, which controls, in step 5~step 10.
Embodiment 3
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of health
Plant root, which washes with water, completely to carry out disinfection, wherein the plant that grass family can be overwintering is Chinese pennisetum, sterilizes tool in step 1
Body is as follows:
Then the first plant root 10s selected by 70% alcohol soaking step 1 uses 2% chlorine with aseptic water washing 3 times
Acid sodium solution impregnates 11min or impregnates 6min with 0.1% mercuric chloride solution, and whole process constantly stirs, and makes plant root and 2%
0.1% mercuric chloride solution of liquor natrii hypochloritis comes into full contact with, and finally uses aseptic water washing 5 times.
Step 2 cuts 2cm wedge shape in Juncao root, cuts depth in the root that grass family is capable of overwintering plant and transfers for 4cm
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by interface；
When step 3, the plant grafted for obtaining step 2 are placed in the environment of temperature is 30~40 DEG C one section of growth
Between, growth time control is 26 days, and ambient humidity is controlled 65%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 31 DEG C and grows a period of time by a generation, and growth time control is 26 days, environment
Humid control is 65%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
Simultaneously is transplanted in the environment of temperature is 31 DEG C, humidity is 65% culture growth a period of time in the root of gramineae plant；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, culture growth a period of time in the environment of by second filial tissue culture transplantation of seedlings to temperature be 31 DEG C, humidity is 65%；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 31 DEG C, humidity is 65% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, culture growth a period of time in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 34 DEG C, humidity is 65%；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 31 DEG C, humidity is 65% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
It is 26 days that growth time, which controls, in step 5~step 10.
Embodiment 4
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of health
Plant root, which washes with water, completely to carry out disinfection, wherein the plant that grass family can be overwintering is Chinese pennisetum, sterilizes tool in step 1
Body is as follows:
Then the first plant root 15s selected by 70% alcohol soaking step 1 uses 2% chlorine with aseptic water washing 3 times
Acid sodium solution impregnates 12min or impregnates 8min with 0.1% mercuric chloride solution, and whole process constantly stirs, and makes plant root and 2%
0.1% mercuric chloride solution of liquor natrii hypochloritis comes into full contact with, and finally uses aseptic water washing 3 times.
Step 2 cuts 2cm wedge shape in Juncao root, cuts depth in the root that grass family is capable of overwintering plant and transfers for 3cm
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by interface；
Step 3, the plant grafted for obtaining step 2, which are placed in the environment of temperature is 35 DEG C, grows a period of time, raw
Control for a long time is 28 days, and ambient humidity is controlled 70%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 35 DEG C and grows a period of time by a generation, and growth time control is 28 days, environment
Humid control is 70%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
Simultaneously is transplanted in the environment of temperature is 35 DEG C, humidity is 70% culture growth a period of time in the root of gramineae plant；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, culture growth a period of time in the environment of by second filial tissue culture transplantation of seedlings to temperature be 35 DEG C, humidity is 70%；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 35 DEG C, humidity is 70% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, culture growth a period of time in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 35 DEG C, humidity is 70%；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 35 DEG C, humidity is 70% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
It is 30 days that growth time, which controls, in step 5~step 10.
Embodiment 5
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of health
Plant root, which washes with water, completely to carry out disinfection, wherein the plant that grass family can be overwintering is Chinese pennisetum, sterilizes tool in step 1
Body is as follows:
Then the first plant root 25s selected by 70% alcohol soaking step 1 uses 2% chlorine with aseptic water washing 3 times
Acid sodium solution impregnates 14min or impregnates 8min with 0.1% mercuric chloride solution, and whole process constantly stirs, and makes plant root and 2%
0.1% mercuric chloride solution of liquor natrii hypochloritis comes into full contact with, and finally uses aseptic water washing 5 times.
Step 2 cuts 2.5cm wedge shape in Juncao root, and cutting depth in the root that grass family is capable of overwintering plant is
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by 3.5cm graft union；
Step 3, the plant grafted for obtaining step 2, which are placed in the environment of temperature is 35 DEG C, grows a period of time, raw
Control for a long time is 30 days, and ambient humidity is controlled 70%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue culture young plant
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 35 DEG C and grows a period of time by a generation, and growth time control is 32 days, environment
Humid control is 75%；
The healthy plant that step 5, selection are obtained through step 4 chooses first filial generation grafting in step 1 further according to step 2
Simultaneously is transplanted in the environment of temperature is 35 DEG C, humidity is 75% culture growth a period of time in the root of gramineae plant；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue culture young plant
Two generations, in the environment of by second filial tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80% at one section of culture growth
Between；
Second filial, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Simultaneously is transplanted in the environment of temperature is 38 DEG C, humidity is 70% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue culture young plant
Three generations, culture growth a period of time in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 38 DEG C, humidity is 75%；
Child three generations, the grass family chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Simultaneously is transplanted in the environment of temperature is 35 DEG C, humidity is 750% culture growth a period of time in the root of plant；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant
Four generations, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
It is 35 days that growth time, which controls, in step 5~step 10.
Claims (6)
1. a kind of overwintering Juncao new varieties method for culturing seedlings, which is characterized in that be specifically implemented according to the following steps:
Step 1, the good Juncao of selection growth conditions and grass family can be overwintering plant, and by choose two kinds of healthy plants
Root, which washes with water, completely to carry out disinfection；
Step 2 cuts 2~3cm wedge shape in Juncao root, and cutting depth in the root that grass family is capable of overwintering plant is 3~4cm
Then Juncao is grafted and is capable of the root of overwintering plant in grass family, then ties up fixation by graft union；
Step 3, the plant grafted for obtaining step 2, which are placed in the environment of temperature is 30~40 DEG C, grows a period of time, ring
Border humid control is 60~80%；
The blade that healthy plant is newly grown in step 4, selection step 3 carries out tissue cultures, obtains corresponding tissue-cultured seedling first filial generation,
Tissue-cultured seedling first filial generation is placed in the environment of temperature is 30~40 DEG C and grows a period of time, ambient humidity is controlled 60~80%；
First filial generation, the grass chosen in step 1 is grafted further according to step 2 by the healthy plant that step 5, selection are obtained through step 4
Simultaneously is transplanted in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time in the root of section plant；
The blade that healthy plant is newly grown in step 6, selection step 5 carries out tissue cultures, obtains corresponding tissue-cultured seedling second filial,
Culture growth a period of time in the environment of by second filial tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80%；
Second filial, the gramineae plant chosen in step 1 is grafted further according to step 2 by healthy plant in step 7, selection step 6
Root and transplant in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time；
The blade that healthy plant is newly grown in step 8, selection step 8 carries out tissue cultures, obtains corresponding tissue-cultured seedling child three generations,
Culture growth a period of time in the environment of by child three generations tissue culture transplantation of seedlings to temperature be 30~40 DEG C, humidity is 60~80%；
Child three generations, the gramineae plant chosen in step 1 is grafted further according to step 2 by healthy plant in step 9, selection step 8
Root and transplant in the environment of temperature is 30~40 DEG C, humidity is 60~80% culture growth a period of time；
The blade that healthy plant is newly grown in step 10, selection step 9 carries out tissue cultures, obtains corresponding tissue culture young plant four
Generation, what in four generation of tissue culture young plant, as finally cultivated can be overwintering Juncao new varieties.
2. a kind of overwintering Juncao new varieties method for culturing seedlings according to claim 1, which is characterized in that standing grain in the step 1
Undergraduate course can be overwintering plant be Chinese pennisetum.
3. a kind of overwintering Juncao new varieties method for culturing seedlings according to claim 1, which is characterized in that disappear in the step 1
Poison is specific as follows:
Then first 10~30s of plant root selected by 70% alcohol soaking step 1 is used 2% time with aseptic water washing 2~3 times
Sodium chlorate solution impregnates 10~15min or impregnates 5~10min with 0.1% mercuric chloride solution, and whole process constantly stirs, makes to plant
Strain root comes into full contact with 2% liquor natrii hypochloritis, 0.1% mercuric chloride solution, finally uses aseptic water washing 3~5 times.
4. a kind of overwintering Juncao new varieties method for culturing seedlings according to claim 1, which is characterized in that transferred in the step 3
The plant connected growth time control in the environment of temperature is 30~40 DEG C is 25~35 days.
5. a kind of overwintering Juncao new varieties method for culturing seedlings according to claim 1, which is characterized in that group in the step 4
Training growth time control in the environment of temperature is 30~40 DEG C of a young plant generation is 25~35 days.
6. a kind of overwintering Juncao new varieties method for culturing seedlings according to claim 1, which is characterized in that the step 5~step
It is 25~35 days that growth time, which controls, in rapid 10.
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