CN110253881B - Multi-nozzle 3D biological printing device and printing method - Google Patents
Multi-nozzle 3D biological printing device and printing method Download PDFInfo
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- CN110253881B CN110253881B CN201910563558.3A CN201910563558A CN110253881B CN 110253881 B CN110253881 B CN 110253881B CN 201910563558 A CN201910563558 A CN 201910563558A CN 110253881 B CN110253881 B CN 110253881B
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- 238000007639 printing Methods 0.000 title claims abstract description 70
- 238000000034 method Methods 0.000 title claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 172
- 239000002184 metal Substances 0.000 claims abstract description 7
- 229910052751 metal Inorganic materials 0.000 claims abstract description 7
- 210000000056 organ Anatomy 0.000 claims description 19
- 238000003860 storage Methods 0.000 claims description 15
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000005192 partition Methods 0.000 claims description 6
- 238000004113 cell culture Methods 0.000 claims description 3
- 210000004748 cultured cell Anatomy 0.000 claims description 3
- 238000007493 shaping process Methods 0.000 claims description 3
- 238000010146 3D printing Methods 0.000 abstract description 4
- 238000001125 extrusion Methods 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract 1
- 210000001519 tissue Anatomy 0.000 description 18
- 210000004027 cell Anatomy 0.000 description 15
- 238000005516 engineering process Methods 0.000 description 7
- 239000012620 biological material Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 201000003101 Coloboma Diseases 0.000 description 1
- 206010053159 Organ failure Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000005253 cladding Methods 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000012774 insulation material Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000004872 soft tissue Anatomy 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/10—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C64/00—Additive manufacturing, i.e. manufacturing of three-dimensional [3D] objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
- B29C64/20—Apparatus for additive manufacturing; Details thereof or accessories therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y10/00—Processes of additive manufacturing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B33—ADDITIVE MANUFACTURING TECHNOLOGY
- B33Y—ADDITIVE MANUFACTURING, i.e. MANUFACTURING OF THREE-DIMENSIONAL [3-D] OBJECTS BY ADDITIVE DEPOSITION, ADDITIVE AGGLOMERATION OR ADDITIVE LAYERING, e.g. BY 3-D PRINTING, STEREOLITHOGRAPHY OR SELECTIVE LASER SINTERING
- B33Y30/00—Apparatus for additive manufacturing; Details thereof or accessories therefor
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Manufacturing & Machinery (AREA)
- Physics & Mathematics (AREA)
- Mechanical Engineering (AREA)
- Optics & Photonics (AREA)
- Materials For Medical Uses (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a multi-nozzle 3D biological printing device and a printing method, belonging to the field of medical instruments; the technical problem to be solved is to provide a bioprinter which carries out 3D printing while culturing cells in an extrusion mode; the technical scheme for solving the technical problem is as follows: a multi-nozzle 3D biological printing device comprises a material bin, a material rotating wheel, a nozzle rotating wheel and a printing platform which are sequentially arranged from top to bottom, wherein the material rotating wheel is movably connected with the nozzle rotating wheel, the material bin is movably arranged in the material rotating wheel, the material rotating wheel and the interior of the nozzle rotating wheel are equally divided into 6 chambers, an electromagnetic metal coil is arranged at the connection part of the material rotating wheel and the nozzle rotating wheel, a lifting frame is arranged below the printing platform, the material bin moves into the nozzle rotating wheel, and then printing is carried out in an extrusion mode; the invention can be widely applied to the field of medical appliances.
Description
Technical Field
The invention discloses a multi-nozzle 3D biological printing device and a printing method, and belongs to the technical field of medical instruments.
Background
Organ transplantation can save lives of a plurality of patients with organ failure or damage, but the technology also has the defects of insufficient organ sources, difficult avoidance of rejection reaction and the like, but with the emergence of 3D bioprinters, the problems have substantial progress, the 3D bioprinter is a device which can position and assemble biological materials or cell units according to the additive manufacturing principle under the drive of a digital three-dimensional mode and manufacture products such as medical instruments, tissue engineering direct products, tissue organs and the like, and meanwhile, the 3D bioprinter is not plastic layer by layer, but utilizes biological materials or cell building blocks layer by layer to manufacture real living tissues, however, the existing cell three-dimensional printing technology is limited by the cell printing technology, most of the existing cell three-dimensional printing technology is used for constructing simple structures by operating cells or materials with single components, and is only limited to certain simple tissues, such as soft tissues, skin tissues, tendon tissues and the like, complex tissues cannot be printed, the current three-dimensional controlled cell assembly is a new technology for manufacturing tissue or organ precursors by positioning and assembling living cells or material units under the direct drive of an anatomical digital model of a tissue organ, and provides brand-new possibility for constructing complex tissues and organs in vitro, but the organs are three-dimensional structures which are composed of different cells and have complex microstructures, so that the multi-cell assembly technology becomes the key for developing the cell assembly technology.
Disclosure of Invention
The invention overcomes the defects in the prior art and provides the bioprinter which can culture cells and perform 3D printing in an extrusion mode.
In order to solve the technical problems, the invention adopts the technical scheme that: a multi-nozzle 3D biological printing device comprises a material bin, a material rotating wheel, a nozzle rotating wheel and a printing platform which are sequentially arranged from top to bottom, wherein the material rotating wheel is movably connected with the nozzle rotating wheel, and the material bin is movably arranged in the material rotating wheel;
the material rotating wheel comprises a material rotating shaft and a material shell, and the material rotating shaft is connected with the material shell through a material partition plate;
the nozzle rotating wheel comprises a nozzle rotating shaft and a nozzle shell, and the nozzle rotating shaft is connected with the nozzle shell through a nozzle partition plate;
the material rotating shaft and the nozzle rotating shaft are arranged up and down on the same axis, and the material shell and the nozzle shell have the same structure;
the material bin comprises a material storage pipe, a conical discharge hole is formed in the lower portion of the material storage pipe, a piston is arranged on the upper portion of the material storage pipe, and a lifting plate is arranged on the side face of the material storage pipe.
The material shell and the nozzle shell are both columnar shells.
The material division board evenly divides the inner cavity of the material shell into 6 chambers, and the nozzle division board evenly divides the nozzle shell into 6 chambers.
And an electromagnetic metal coil is arranged at the joint of the material rotating wheel and the nozzle rotating wheel.
And a lifting frame is arranged below the printing platform.
The material bin is a material bin with a heating sleeve.
A multi-nozzle 3D biological printing method comprises the following steps:
1) establishing a 3D model of the living tissue or organ to be printed through mathematical modeling, simultaneously carrying out cell culture synchronously, and loading the cultured cells into a temperature control material bin with a heating sleeve;
2) establishing a living tissue or organ model frame, dividing the frame into six parts, and arranging the six parts in corresponding areas of a printing platform according to a 3D model so as to facilitate the early-stage shaping of tissues and organs;
3) sequentially loading the material bins into six cavities of the material rotating wheel, controlling the material shaft to rotate at the same time, ensuring that the six material bins of the material rotating wheel correspond to the six cavity bodies of the nozzle rotating wheel one by one, and moving the material rotating wheel to be right above the nozzle rotating wheel;
4) slowly putting down the material rotating wheel, and butting the material rotating wheel with the nozzle rotating wheel;
5) the material storage pipe is moved into the nozzle rotating wheel by a lifting plate on the side surface of the material bin in the material rotating wheel, and the material storage pipe is transferred into the nozzle rotating wheel;
6) separating the material rotating wheel from the nozzle rotating wheel to carry out secondary material transfer;
7) the upper part of a material bin on the nozzle runner is connected with an air pressure control valve, and the lower nozzle part is connected with an output controller;
8) moving the printing platform to be right below the nozzle rotating wheel, and realizing fixed-point placement of a printing material, accurate forming of a printing target and completion of a printing task through rotation, stretching, lifting and opening and closing of the printing platform and the printing nozzle;
9) after printing is finished, separating an air pressure control valve at the upper part of the nozzle rotating wheel from an output controller at the lower part of the nozzle rotating wheel, taking out the material bin from the nozzle rotating wheel, and leaving the cavity corresponding to the nozzle rotating wheel idle so as to facilitate next printing;
10) and step 3 to step 9 are circulated until the printing is finished.
The moving of the material rotating wheel in the step 3 can use a mechanical arm, and the material bin is taken out in the step 9 can also use a mechanical arm.
Compared with the prior art, the invention has the following beneficial effects: the material runner has a plurality of cavitys with nozzle runner is inside, can realize that the shower nozzle switches, realize that extrusion formula 3D biology prints the printing to complicated organ and polymorphic type cell, for really printing out the tissue organ of extensive application in the future and provide technical support, can realize carrying out the mode that 3D printed while cultivating the cell, guarantee the rapidity and the real-time of printing, and can guarantee the activity of printing the cell, improve the success rate of printing the material, have wide prospect in the aspect of organizing coloboma, in time change, emergency rescue.
Drawings
The invention is further described below with reference to the accompanying drawings.
FIG. 1 is a schematic structural diagram of the present invention.
Detailed Description
As shown in fig. 1, the multi-nozzle 3D bio-printing apparatus of the present invention comprises a material bin, a material rotating wheel, a nozzle rotating wheel, and a printing platform, which are sequentially arranged from top to bottom, wherein the material rotating wheel is movably connected with the nozzle rotating wheel, and the material bin is movably disposed in the material rotating wheel; the material rotating wheel comprises a material rotating shaft and a material shell, and the material rotating shaft is connected with the material shell through a material partition plate; the nozzle rotating wheel comprises a nozzle rotating shaft and a nozzle shell, and the nozzle rotating shaft is connected with the nozzle shell through a nozzle partition plate; the material rotating shaft and the nozzle rotating shaft are arranged up and down on the same axis, and the material shell and the nozzle shell have the same structure; the material storehouse includes the material storage pipe, and material storage pipe lower part is provided with the toper discharge gate, and upper portion is provided with the piston, and the side is provided with the lifter plate, material shell and nozzle shell be the column shell, the material division board evenly separate material shell inner chamber for 6 cavities, the nozzle division board evenly separate nozzle shell for 6 cavities, the department that links to each other of material runner and nozzle runner is provided with electromagnetic metal coil, the print platform below is provided with the lift and contracts the frame, the material storehouse is from the material storehouse of taking the heating jacket.
A multi-nozzle 3D biological printing method comprises the following steps:
1) establishing a 3D model of the living tissue or organ to be printed through mathematical modeling, simultaneously carrying out cell culture synchronously, and loading the cultured cells into a temperature control material bin with a heating sleeve;
2) establishing a living tissue or organ model frame, dividing the frame into six parts, and arranging the six parts in corresponding areas of a printing platform according to a 3D model so as to facilitate the early-stage shaping of tissues and organs;
3) sequentially loading the material bins into six cavities of the material rotating wheel, controlling the material shaft to rotate at the same time, ensuring that the six material bins of the material rotating wheel correspond to the six cavity bodies of the nozzle rotating wheel one by one, and moving the material rotating wheel to be right above the nozzle rotating wheel;
4) slowly putting down the material rotating wheel, and butting the material rotating wheel with the nozzle rotating wheel;
5) the material storage pipe is moved into the nozzle rotating wheel by a lifting plate on the side surface of the material bin in the material rotating wheel, and the material storage pipe is transferred into the nozzle rotating wheel;
6) separating the material rotating wheel from the nozzle rotating wheel to carry out secondary material transfer;
7) the upper part of a material bin on the nozzle runner is connected with an air pressure control valve, and the lower nozzle part is connected with an output controller;
8) moving the printing platform to be right below the nozzle rotating wheel, and realizing fixed-point placement of a printing material, accurate forming of a printing target and completion of a printing task through rotation, stretching, lifting and opening and closing of the printing platform and the printing nozzle;
9) after printing is finished, separating an air pressure control valve at the upper part of the nozzle rotating wheel from an output controller at the lower part of the nozzle rotating wheel, taking out the material bin from the nozzle rotating wheel, and leaving the cavity corresponding to the nozzle rotating wheel idle so as to facilitate next printing;
10) and step 3 to step 9 are circulated until the printing is finished.
The moving of the material rotating wheel in the step 3 can use a mechanical arm, and the material bin is taken out in the step 9 can also use a mechanical arm.
The printing platform is a disc and is divided into six fan-shaped areas with 60-degree internal angles, a model of an object to be printed can be divided into six parts, each nozzle is opposite to one fan-shaped area and is mainly responsible for printing the area, each area can be independently operated in the printing process, when all the six printing areas are formed, the six printing areas are combined together to form a complete tissue or organ, the nozzle wheel is formed by taking a regular hexagon as an external frame and is uniformly cut into six regular triangular prisms, a material bin carrying the printing nozzle can be accommodated in each triangular prism, a lifting groove for lifting the material bin is arranged on the side surface of the material bin, the outer edge on the upper surface of the nozzle wheel is made of a metal material (the outer edge on the lower surface of the material wheel is also made by the same method), when the distance between the lower surface of the material wheel and the upper surface of the nozzle wheel is smaller than a given value (a distance meter can be added on the lower surface of the, the two metals are internally communicated with the same-direction current, so that the nozzle rotating wheel and the material rotating wheel are tightly adsorbed under the action of a magnetic field generated by the current, the purpose of lifting the material bin is achieved, when the material bin is smoothly placed at the designated position of the nozzle rotating wheel, the metal outer edges are communicated with the reverse current, repulsive force is generated, and the nozzle rotating wheel and the material rotating wheel are assisted to be separated. When the separation distance between the material rotating wheel and the nozzle rotating wheel is larger than or equal to a certain value, the current output is stopped, the material rotating wheel and the nozzle rotating wheel are perfectly separated by the action of the mechanical arm, an air breather connected with controllable air pressure is arranged right above the material bin, the through hole is connected with an air control valve, the controllable adjustment of the air pressure is realized by adjusting the air control valve, the printing speed is further controlled, a layer of heat insulation material is coated on the periphery of the material bin, the temperature is required to be adjustable, the temperature difference of different printing materials on the temperature is met, the material rotating wheel is provided with a right hexagonal body as an external frame, the size of the material rotating wheel is consistent with that of the nozzle rotating wheel, the central shaft controls the positions of the six material bins, the six material bins can rotate around the central shaft, so that the material bin can reach any nozzle rotating wheel, the material rotating wheel and the nozzle rotating, cladding one deck temperature control material around the material storehouse, the metal sheet of side is opened to the material storehouse accessible, in putting a cavity of appointed material runner with the material storehouse of arm with having prepared, accomplish the loading of material storehouse, the material storehouse is disposable product, only once print the flow, so the material chooses for use biological affinity material, print shower nozzle department through output controller control printing time and progress, this output controller is impulse generator, when printing, can print on one side completely, cultivate the cell on one side, the biomaterial that needs to print is cultivateed good the back, load in the material storehouse, after waiting for last loading and unloading, the material storehouse that has been cultivateed is loaded to the material runner that vacates, thereby guarantee biomaterial's activity.
The embodiments of the present invention have been described in detail with reference to the accompanying drawings, but the present invention is not limited to the above embodiments, and various changes can be made within the knowledge of those skilled in the art without departing from the gist of the present invention.
Claims (8)
1. The utility model provides a biological printing device of many shower nozzles 3D which characterized in that: the printing device comprises a material rotating wheel, a nozzle rotating wheel and a printing platform which are sequentially arranged from top to bottom, wherein the material rotating wheel is movably connected with the nozzle rotating wheel, and a material bin is movably arranged in the material rotating wheel;
the material rotating wheel comprises a material rotating shaft and a material shell, and the material rotating shaft is connected with the material shell through a material partition plate;
the nozzle rotating wheel comprises a nozzle rotating shaft and a nozzle shell, and the nozzle rotating shaft is connected with the nozzle shell through a nozzle partition plate;
the material rotating shaft and the nozzle rotating shaft are arranged up and down on the same axis, and the material shell and the nozzle shell have the same structure;
the material bin comprises a material storage pipe, a conical discharge hole is formed in the lower portion of the material storage pipe, a piston is arranged on the upper portion of the material storage pipe, and a lifting plate is arranged on the side face of the material storage pipe.
2. The multi-nozzle 3D bioprinting device of claim 1, wherein: the material shell and the nozzle shell are both columnar shells.
3. The multi-nozzle 3D bioprinting device of claim 1, wherein: the material division board evenly divides the inner cavity of the material shell into 6 chambers, and the nozzle division board evenly divides the nozzle shell into 6 chambers.
4. The multi-nozzle 3D bioprinting device of claim 1, wherein: and an electromagnetic metal coil is arranged at the joint of the material rotating wheel and the nozzle rotating wheel.
5. The multi-nozzle 3D bioprinting device of claim 1, wherein: and a lifting frame is arranged below the printing platform.
6. The multi-nozzle 3D bioprinting device of claim 1, wherein: the material bin is a material bin with a heating sleeve.
7. A multi-nozzle 3D biological printing method is characterized by comprising the following steps:
1) establishing a 3D model of the living tissue or organ to be printed through mathematical modeling, simultaneously carrying out cell culture synchronously, and loading the cultured cells into a temperature control material bin with a heating sleeve;
2) establishing a living tissue or organ model frame, dividing the frame into six parts, and arranging the six parts in corresponding areas of a printing platform according to a 3D model so as to facilitate the early-stage shaping of tissues and organs;
3) sequentially loading the material bins into six cavities of the material rotating wheel, controlling the material shaft to rotate at the same time, ensuring that the six material bins of the material rotating wheel correspond to the six cavity bodies of the nozzle rotating wheel one by one, and moving the material rotating wheel to be right above the nozzle rotating wheel;
4) slowly putting down the material rotating wheel, and butting the material rotating wheel with the nozzle rotating wheel;
5) the material storage pipe is moved into the nozzle rotating wheel by a lifting plate on the side surface of the material bin in the material rotating wheel;
6) separating the material rotating wheel from the nozzle rotating wheel to carry out secondary material transfer;
7) the upper part of a material bin on the nozzle runner is connected with an air pressure control valve, and the lower nozzle part is connected with an output controller;
8) moving the printing platform to be right below the nozzle rotating wheel, and realizing fixed-point placement of a printing material, accurate forming of a printing target and completion of a printing task through rotation, stretching, lifting and opening and closing of the printing platform and the printing nozzle;
9) after printing is finished, separating an air pressure control valve at the upper part of the nozzle rotating wheel from an output controller at the lower part of the nozzle rotating wheel, taking out the material bin from the nozzle rotating wheel, and leaving the cavity corresponding to the nozzle rotating wheel idle so as to facilitate next printing;
10) and step 3 to step 9 are circulated until the printing is finished.
8. The multi-nozzle 3D bioprinting method of claim 7, wherein: the moving of the material rotating wheel in the step 3 uses a mechanical arm, and the taking out of the material bin in the step 9 also uses the mechanical arm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910563558.3A CN110253881B (en) | 2019-06-26 | 2019-06-26 | Multi-nozzle 3D biological printing device and printing method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910563558.3A CN110253881B (en) | 2019-06-26 | 2019-06-26 | Multi-nozzle 3D biological printing device and printing method |
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| Publication Number | Publication Date |
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| CN110253881A CN110253881A (en) | 2019-09-20 |
| CN110253881B true CN110253881B (en) | 2021-05-28 |
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| CN201910563558.3A Active CN110253881B (en) | 2019-06-26 | 2019-06-26 | Multi-nozzle 3D biological printing device and printing method |
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Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104924614B (en) * | 2015-06-08 | 2017-08-25 | 浙江大学 | Towards the biological 3D printing shower nozzle of rotating switching type and its method of many file printings |
| WO2017113186A1 (en) * | 2015-12-30 | 2017-07-06 | 四川蓝光英诺生物科技股份有限公司 | Nozzle assembly of biological printer and biological printer |
| CN105796206B (en) * | 2016-05-07 | 2017-08-04 | 浙江大学 | The biological 3D printing equipment of many shower nozzles of a kind of automatic blending and batch mixing and control method |
| CN107028681B (en) * | 2017-04-11 | 2018-11-30 | 清华大学深圳研究生院 | A kind of 3D printing device and method of tissue engineering bracket |
| CN207758151U (en) * | 2018-01-11 | 2018-08-24 | 刘晓军 | More nozzle switching devices, 3D printing head and 3D printer |
| CN108673891B (en) * | 2018-06-14 | 2024-03-12 | 浙江大学 | Multi-runner spray head structure of portable biological 3D printer |
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Effective date of registration: 20240429 Address after: Room 703, Building 1, No. 430 Miaoqiao Road, Pudong New Area, Shanghai, 201315 Patentee after: Lanbaotai (Shanghai) Biopharmaceutical Co.,Ltd. Country or region after: China Address before: 030024 No. 79 West Main Street, Taiyuan, Shanxi, Yingze Patentee before: Taiyuan University of Technology Country or region before: China |
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