CN206127271U - Biological body printing device that founds - Google Patents

Abstract

The utility model relates to a biological body printing device that founds, it includes: support material, first coating part, reagent feeding device and placement machine construct, and first coating part can be regional in order to form the bond line predetermineeing of support material with the coating of first reagent, reagent feeding device be used for to the supply of first coating part first reagent, the placement machine structure can be placed the bioprinting unit on the bond line to make the bioprinting unit adhere on the bond line. The utility model discloses biological body printing device that founds has realized certainly existing the bond line between support material and the bioprinting unit, and some bioprinting units that has been favorable to avoiding take place the condition of sliding or dropping on the support material.

Description

Biological construct printing equipment

Technical field

This utility model is related to biometric print technical field, more particularly to a kind of biological construct printing equipment.

Background technology

3D biometric prints refer to the principle and method by 3D printing, by biomaterial (including natural biologic material and conjunction Into biomaterial or cell solution) print and become the three-dimensional structure of design, common 3D printing technique is different from, biological 3D beats The biological tissue of print technology production or organ also have certain biological function, need to be that the further growth of cell and tissue is carried For condition, just because of above-mentioned characteristic, biological 3D printing technique is faced with many specific technical problems, especially in development It is preparation and the Printing Problem of biological prepared Chinese ink.

Traditional 3D biometric print methods are typically with the mode of continuous printing and biological brick are exported to target location simultaneously Complete to print, the more than biometric print unit of biomaterial of continuous printout in print procedure itself, it is glutinous also between it Mixture, hydrogel, these materials are printed together, raw because adhesive and hydrogel have the situation of skewness The uncoated adhesive in subregion of thing print unit so that part biological print unit slides on the support or falls Fall, exist that dispersibility is poor, biometric print unit easily occurs being interrupted discontinuous situation.

In order to biometric print unit is reliably printed on the support, a kind of existing mode is Publication No. The Chinese utility model patent application of CN103703119A, it passes through the means for building " sword mountain " in advance, prepares cell ball, and will Cell ball by puncture means cell ball string on sword mountain, so that it grows and connects to form biological tissue.The program It is disadvantageous in that：

1st, during taking out during biological tissue is built and after structure biological tissue, all easily damage Cell, specifically, when biological tissue is built, it is needed by pin puncture cyton, and so cell therein will certainly be made Into serious infringement, in addition, after structure forms biological tissue's precursor, the process of taking-up can be subject to the mechanical mechanical resistance from pin Hinder, easily the structure and cell of the firm biological tissue's precursor for generating are caused to damage.

2nd, higher for the requirement of cyton particle diameter, needing the cyton of greater particle size could implement, specifically, because its Cyton particle diameter is greater than the diameter of pin, and needs into the big particle diameter of geometry multiple, for the cyton of small particle, takes Put that difficulty is big, be difficult to effectively puncture, further, since the cyton for needing is than larger, so thin in individual cells body Born of the same parents' quantity will be very more, so occur higher for the supply requirement of nutrition in individual cells body.

3rd, it is higher for the uniformity requirements of cyton, if cyton itself particle diameter difference is just larger, if adjacent is thin Cell space is too big, then easily mutually extruding between cell, steric configuration and cell growth is affected, if cyton is too little, it is difficult to each other Connection, can also affect steric configuration and cell growth.

4th, such scheme needs previously prepared " sword mountain ", needs, according to standby tissue of drawing up, to determine that the particle diameter of cyton is big Little and distribution situation, so as to the supporting sword mountain for preparing gap between corresponding thickness needle body, needle body distribution and needle body, not only its own system Make with regard to very complicated trouble, and limited by sword mountain needle body arrangement mode, it is corresponding spatially in a certain needle body, can only make With unified cyton, it is impossible to which different size of cyton is used in combination, such mode limits the complexity of cell tissue Degree and the degree of freedom for building and degree of flexibility, in addition, such mode is also difficult to mutually be tied by the microsphere of different-grain diameter size The mode of conjunction generates biological tissue (big particle diameter spheroid, small particle spheroid combine).

Additionally, people are mixed and made into biological prepared Chinese ink using cell and biocompatible materialses, will be raw by way of extruding Thing prepared Chinese ink builds mathematical model printing shaping according to the three-dimensional of default target print object.In order to realize reasonable molding, This mode is needed using the higher biocompatible materialses of ratio of viscosities.Therefore in print procedure, the machine for overcoming shower nozzle is needed Tool external force acts on the biological print ink higher to realize ratio of viscosities, and in print procedure, the cell in biological prepared Chinese ink would generally Damage than larger, and this mode, in order to ensure enough cell survival amounts, need to use substantial amounts of cell, it is impossible to realize The exact placement of cell.In addition, the arrangement, sky in actual print procedure, between biometric print unit and biometric print unit Between the distance between position, brick etc. the growth of actual tissue or blood vessel is all had a great impact.

Utility model content

To overcome above technological deficiency, the technical problem that this utility model is solved to be to provide a kind of biological construct and print dress Put, the situation for avoiding part biological print unit from sliding on the support or drop can be conducive to, and can be preferably Keep the biological activity of biometric print unit.

To solve above-mentioned technical problem, this utility model provides a kind of biological construct printing equipment, and it includes：Support Thing, the first application member, reagent feedway and placement mechanism, wherein,

First application member is coated in the predeterminable area of holder to form adhesive layer for the first reagent；

Reagent feedway is used to the first reagent is supplied to the first application member；And

Placement mechanism is used to for biometric print unit to be placed on adhesive layer, so that biometric print unit is bonded to bonding On layer so that at least form a part for biological construct.

Further, also including the second application member, the second application member can in advance be applied in the predeterminable area of holder Spacer medium is covered to form spacer medium layer, adhesive layer is coated on the support by spacer medium layer.

Further, placement mechanism includes suction elements, and suction elements can draw biometric print unit and be moved to viscous Close and discharge at layer position biometric print unit, to realize that biometric print unit is bonded on adhesive layer.

Further, suction elements can in advance draw liquid and make the suction end of suction elements form liquid film, and biology is beaten Impression unit is drawn after part is drawn and is maintained at the suction end of suction elements and contacts with liquid film.

Further, suction elements include that at least one draws position, and each is drawn position and only draws single biology every time Print unit.

Further, the print unit test kit for holding biometric print unit is also included, in print unit test kit Multiple hole positions are provided with, each hole position is used to deposit single biometric print unit.

Further, suction elements include several absorption positions, and several drawing section potential energies are enough to beat the several biologies drawn Impression unit is discharged to adhesive layer simultaneously.

Further, also including controlling organization and for carrying out position to the biometric print unit in print unit test kit The visual system of seizure, visual system can send feedback signal after single biometric print unit is captured to controlling organization, Controlling organization controls suction elements and is moved in print unit test kit corresponding position and draws biology to beat according to feedback signal Impression unit, controls suction elements and is moved to release biometric print unit at adhesive layer position then.

Further, also including the 3rd application member, in biological construct surface spraying moulding material or fid Material.

Further, biological construct is lumen organization's structure, and holder is swingle, and predeterminable area is the outer of swingle Wall.

Preferably, lumen organization's structure is blood vessel precursor.

Thus, based on above-mentioned technical proposal, this utility model biological construct printing equipment is by the default of holder To form adhesive layer, biometric print unit is bonded on adhesive layer the reagent of region coating first, that is to say, that beat on the support Before print biometric print unit, the predeterminable area in holder has smeared the adhesive layer that can bond biometric print unit, real Show and certainly exist adhesive layer between holder and biometric print unit, improved existing biometric print unit with adhesive quilt There is the situation of the uncoated adhesive in subregion of biometric print unit in the mode for printing together, be conducive to avoiding portion The situation that decomposing biological print unit slides on the support or drops, it is reasonable in order to realize compared in traditional prints Molding needs and adopts the scheme that the higher biocompatible materialses of ratio of viscosities and the mixing of biometric print unit are extruded, and this practicality is new Type biological construct printing equipment separately carries out adhesive layer and biometric print unit to be layered printing and arrangement, it is to avoid Mobility is good when mixing material is extruded, discharging is discontinuous, easily the problem of blocking outlet, it is to avoid in print procedure in order to gram The mechanical external force for taking shower nozzle is acted on and the cell in biological prepared Chinese ink made realizing the higher biometric print unit of ratio of viscosities to print Into the problem compared with macrolesion；Additionally, compared to the scheme on existing " sword mountain ", this utility model biological construct printing equipment will Biometric print unit is adhered on the support by adhesive layer, it is to avoid made for the puncture of cyton in biometric print unit Into damage, be conducive to keeping the biological activity of biometric print unit.

Description of the drawings

Accompanying drawing described herein is used for providing further understanding to of the present utility model, constitutes the part of the application, Schematic description and description of the present utility model be only used for explain this utility model, do not constitute to it is of the present utility model not Work as restriction.In the accompanying drawings：

Fig. 1 is the support by this utility model biological construct printing equipment layered coated spacer medium layer and adhesive layer The structural representation of thing；

Fig. 2 is the mistake of suction elements crawl and placement biometric print unit in this utility model biological construct printing equipment Journey schematic diagram；

Fig. 3 is the print procedure schematic diagram of the first printing type in this utility model biological construct printing equipment；

Fig. 4 is the print procedure schematic diagram of second printing type in this utility model biological construct printing equipment；

Fig. 5 is the overall structure diagram of this utility model biological construct printing equipment embodiment；

Fig. 6 is the structural representation that positioning element is driven in this utility model biological construct printing equipment embodiment；

Fig. 7 is the structural representation of the first nozzle component in this utility model biological construct printing equipment embodiment；

Fig. 8 is the structural representation of the second nozzle component in this utility model biological construct printing equipment embodiment；

Fig. 9 is the structural representation of print unit test kit in this utility model biological construct printing equipment embodiment；

Figure 10 is the depression angle of print unit test kit in this utility model biological construct printing equipment embodiment Structural representation；

Figure 11 is the structural representation of the raw instrument of rotation in this utility model biological construct printing equipment embodiment.

Specific embodiment

Below by drawings and Examples, the technical solution of the utility model is described in further detail.

Specific embodiment of the present utility model is for the ease of asking design of the present utility model, the technology for being solved Topic, the technical characteristic for constituting technical scheme and the technique effect for bringing have further description.It should be noted that for this The explanation of a little embodiments is not constituted to restriction of the present utility model.Additionally, in following embodiment of the present utility model As long as the technical characteristic being related to does not constitute each other conflict and just can be mutually combined.

In description of the present utility model, it is to be understood that limit zero using the word such as " first ", " second " Part, it is only for be easy to distinguish corresponding parts, such as without Stated otherwise, above-mentioned word does not have particular meaning, Therefore it is not intended that the restriction to this utility model protection domain.

As used herein, term " biological construct " refers to, artificial constructed, the two-dimentional or three-dimensional containing cell Structure.In certain preferred aspects, biological construct is three-dimensional construct, tissue precursor, artificial organ or artificial Organ.

Term " biometric print unit " to be referred to and build a kind of substantially single of biological construct by device of the present utility model Unit, it can be used for multiple fields, such as biometric print (such as 3D biometric prints), organizational project, regenerative medicine field.

Preferably, biometric print unit is microcapsule.

Preferably, microcapsule is biological brick.

Preferably, the structure and composition that biological brick of the present utility model can have is：Celliferous stratum nucleare is wrapped, wherein, Cell can be grown, bred, broken up or be migrated, and stratum nucleare is made up of Biodegradable material, and the life for cell is lived It is dynamic that required material is provided；With, the shell of stratum nucleare is encapsulated, shell is located at outside, is made up of Biodegradable material, and for interior The stratum nucleare in portion and cell provide mechanics protection, and the biological brick of this preferred structure can be used as the base unit of biological 3D printing.

Preferably, the particle diameter of biometric print unit of the present utility model is 0.5mm～3mm；

Preferably, biometric print unit of the present utility model is gel state microgranule；Specifically, device institute of the present utility model The stratum nucleare and/or shell of the biometric print unit (biological brick) of preparation can be gel state.Biometric print list of the present utility model Unit can include hydrogel.

Preferably, hydrogel includes alginate, agarose, gelatin, shitosan or other water solublity or hydrophilic polymer Thing.

Preferably, biometric print unit under physiological environment (such as 4-37 DEG C, for example pH is between 6-8) have it is stable Structure, it is preferably biological brick；Biometric print unit can be various and exist as a mixture that it can be each only It is on the spot spherical or any desired shape (such as cube, rectangular prism, six prisms, cylinder, or irregular shape Shape), it is preferable that the size of biometric print unit is each independently 20-2000 μm, such as 30-1900 μm, 40-1800 μm, 50-1700 μm, 60-1600 μm, 70-1500 μm, 80-1400 μm, 90-1300 μm, 100-1200 μm, 200-1000 μm, 300- 800 μm, 400-600 μm, 100-500 μm；Biometric print unit can also be each independently solid or semisolid, such as gel State.

Biometric print unit can be present as a mixture.Biometric print unit can be detached microcapsule.It is biological Print unit can be arranged in a reservoir.

As used in this article, term " biometric print " is referred to：Using biomaterial (including but not limited to, biomolecule Such as protein, lipid, nucleic acid and metabolite；Cell such as cell solution, celliferous gel, cell suspending liquid, cell Concentrate, many cells aggregation and multicell；Subcellular structure such as organelle and cell membrane；Related to biomolecule divides The biomolecule or the analog of biomolecule of sub such as synthesis) printing.As used in this article, term " printing " refers to, The process of deposition materials in predetermined patterns.In this utility model, biometric print preferably by with automatically or half from The device dynamic, computer assisted three-dimensional prototype device (such as biometric print machine) matches is realizing.However, in this practicality In new, " printing " (such as biometric print) can be carried out by various devices, including but not limited to, using printer (for example 3D printer or biometric print machine) printed；Carry out beating using automatization or non-automated mechanical process (rather than printer) Print；Printed by manual placement or manual deposition (such as using pipettor).

As used herein, term " microcapsule " refers to, the micro structure (example containing cell and biocompatible materialses Such as, micron order is to millimetre-sized structure), wherein, cell is wrapped in the biocompatible materialses.It is of the present utility model micro- Capsule under physiological environment (such as 4-37 DEG C, such as pH between 6-8, such as under the hydrodynamic shear of physiological environment) have it is steady Fixed structure.Preferably, the microcapsule mechanical strength broken with microcapsule is not resulted in absorption or extruding.

As used in this article, term " tissue " is referred to and is made up of homomorphosis or similar, function identical cell mass Cell aggregate, and generally also include the material (referred to as intercellular substance, such as substrate, fiber etc.) of acellular form.Tissue May include one or more cell.

As used in this article, term " artificial organ " refers to, is not to be generated by natural tissues or growth course and shape Into tissue.Artificial organ can be the tissue of artificial manufacture, e.g. carry out cultivating the tissue for obtaining to artificial organ precursor.

As used in this article, term " artificial organ precursor " is referred to comprising holder and multiple of the present utility model micro- The object of capsule, wherein, at least one microcapsule is bonded with holder.In certain embodiments, artificial organ precursor includes support Thing and the biological construct built by microcapsule.In certain embodiments, artificial organ precursor of the present utility model culture, After the operating procedures such as induction, artificial organ can be formed.

As used in this article, term " bonding " is referred to and do not occur relative displacement.In certain embodiments, microcapsule or life Thing construct is bonded with holder, refers to that microcapsule or biological construct are combined on the support.

As used in this article, term " holder " referred in artificial organ precursor of the present utility model, with microcapsule or by The biological construct bonding that microcapsule is constituted, have effigurate object.Holder can provide corresponding region, make biology Construct is completely fixed (adhesion) thereon.

As used in this article, term " tube chamber " is referred to and is shaped as tubulose, such as organ with hollow cavity, circulation pipe Chamber, digestive tract cavity, breathing tube chamber, urinary system tube chamber or reproduction tube chamber, such as blood vessel, esophaguses, trachea, stomach, bile duct, intestinal (including Small intestinal and large intestine, such as duodenum, jejunum, ileum, caecum (including vermiform appendix), ascending colon, right colic flexure, transverse colon, colon Zuo Qu, descending colon, sigmoid colon, rectum), fallopian tube, deferent duct, ureter, bladder or lymphatic vessel).

As used in this article, term " artificial blood vessel " refers to the replacement vessels of artificial manufacture, and it is typically tubulose. In some embodiments, artificial blood vessel is used to rebuild the blood vessel of narrow, inaccessible, expansion, damage or deformity or be repaired. In some embodiments, artificial blood vessel carries out cultivating obtaining to tubular artificial tissue precursor of the present utility model.

As used in this article, term " biocompatible materialses " is referred to：Material (and its catabolite) is for cell It is avirulent, and in implantation host (such as human body) afterwards and host compatibility, does not result in significant or serious secondary work With, for example, toxic action will not be caused to host (such as tissue), immunological rejection, the allergy of host will not be caused Reaction or inflammatory reaction etc..

As used herein, term " Biodegradable material " refers to such material, and it can be by cell or life Object is degraded and is absorbed, and its catabolite is biocompatibility.Such material can be that natural origin (is for example originated In animals and plants), or synthetic.

In an illustrative examples of biological construct printing equipment, with reference to shown in Fig. 1～Figure 11, biological construct Printing equipment includes：Holder 29, the first application member 21, reagent feedway and placement mechanism.First application member 21 The first reagent provided by reagent feedway (not shown) can be coated in the predeterminable area of holder 29 to be formed Adhesive layer B.Placement mechanism can be placed on biometric print unit C on adhesive layer B, so that biometric print unit C is bonded to On adhesive layer B.

In the schematic embodiment, by arranging holder 29, the first application member 21 and placement mechanism, support Thing 29 as print matrix be used for support biometric print unit C, by the first application member 21 holder 29 predeterminable area The first reagent is coated to form adhesive layer B, then placement mechanism is placed on biometric print unit C on adhesive layer B so that biological Print unit C is bonded on adhesive layer B, that is to say, that before printing biometric print unit C on holder 29, is being supported The predeterminable area of thing 29 has smeared the adhesive layer B that can bond biometric print unit C, realizes holder 29 and biometric print unit The adhesive layer B for bonding biometric print unit C is certainly existed between C, the same adhesive of existing biometric print unit is improved There is the situation of the uncoated adhesive in subregion of biometric print unit in the mode for being printed together, be conducive to avoiding The situation that part biological print unit slides on the support or drops.

The higher biocompatibility of ratio of viscosities is adopted compared in traditional prints in order to realize reasonable molding to need The scheme of material and biometric print unit mixing extrusion, this utility model biological construct printing equipment can by adhesive layer B and Biometric print unit C separately carries out being layered printing and arrangement, it is to avoid mobility is good when mixing material is extruded, discharging Discontinuously, the easy problem of blocking outlet, it is to avoid in order to the mechanical external force for overcoming shower nozzle acts on realizing gluing in print procedure The higher biometric print unit of degree prints and the problem compared with macrolesion is caused to the cell in biological prepared Chinese ink.

Compared to the scheme on existing " sword mountain ", this utility model biological construct printing equipment leads to biometric print unit Cross adhesive layer to adhere on the support, without the need for arranging pin, it is to avoid cause for the puncture of cyton in biometric print unit Damage, be conducive to keeping the biological activity of biometric print unit.And this utility model uniformly for biometric print unit Ask relatively low, only biometric print unit need to be bonded on adhesive layer, print unit can be set on demand in print procedure Between spacing, it is to avoid the mutual extruding between adjacent biological print unit.Additionally, this utility model is without the need for previously prepared " sword Mountain ", thus without the need for considering the set location on " sword mountain ", user can at any time change biometric print unit according to practical situation Distribution situation, is conducive to improving the printing degree of freedom and degree of flexibility of biological construct, is beaten by the biology of different-grain diameter size Impression unit combines to form various biological construct.

In an improved embodiment of this utility model biological construct printing equipment, with reference to shown in Fig. 1 and Fig. 7, Biological construct printing equipment also includes the second application member 16, and the second application member 16 can be in the predeterminable area of holder 29 In advance to form spacer medium layer A, adhesive layer B is coated on holder 29 coating spacer medium by spacer medium layer A.Applying Cover and spacer medium is coated in advance to form isolation in the predeterminable area of holder 29 by the second application member 16 before adhesive layer B Dielectric layer A, then adhesive layer B is coated on spacer medium layer A by the first application member 21, coat the mesh of spacer medium layer A Be be easy to print after the completion of biological construct is split away off from holder 29 is overall.Preferably, spacer medium includes temperature Quick property hydrogel, on the one hand, using the Thermo-sensitive of temperature-sensitive hydrogel, print the biological construct that completes can very easily from Integrally split away off on holder 29, on the other hand, temperature-sensitive hydrogel can facilitate adhesive layer B and biometric print unit C Absorption is on holder 29, it is ensured that biometric print unit C does not fall off.

For the version of placement mechanism, placement mechanism can be traditional continuous printing head, continuous printing head Biometric print unit C is continuously printed and is sprayed on adhesive layer B, it is also possible to preferably by the version of crawl-placement, I.e.：In a preferred embodiment, placement mechanism includes suction elements 15, and suction elements 15 can draw biometric print unit C is simultaneously moved to release biometric print unit C at adhesive layer B location, so as to biometric print unit C is bonded on adhesive layer B.Inhale The modes of emplacement that part 15 can be realized capturing-placing is taken, on the one hand can solve the problems, such as precisely to print and arrangement, printed Spacing between print unit can be set on demand in journey, it is to avoid the mutual extruding between adjacent biological print unit.The opposing party Face avoids the problems such as traditional prints mode shower nozzle in print procedure is blocked, brick arrangement is uneven.Preferably, suction elements 15 are wrapped At least one absorption position is included, each is drawn position and only draws single biometric print unit C every time, so as to realize life well Thing print unit C's accurately puts, and biometric print unit C is placed in one by one on adhesive layer B is then bonded, and realizes The printing manufacture of high-precision biological construct.Wherein, suction elements 15 can include an absorption position, it is also possible to preferably Ground includes several absorption positions, and several drawing section potential energies are enough to discharge the multiple biometric print unit C for drawing to adhesive layer simultaneously B, is so conducive to improving printing effect, saves the time-write interval.

For how suction elements 15 realize absorption and release to biological print unit C, in a preferred embodiment In, with reference to Fig. 2 and Fig. 7, suction elements 15 can in advance draw liquid and make the suction end of suction elements 15 form liquid film, biological Print unit C is drawn after part 15 is drawn and is maintained at the suction end of suction elements 15 and contacts with liquid film.In the present embodiment Suction elements are liquid-transfering gun, and suction end is in particular at the muzzle of liquid-transfering gun, and liquid film is formed by the muzzle of liquid-transfering gun by air-breathing, In remaining embodiment, it would however also be possible to employ pipette, burette, syringe etc. are realizing.In advance in suction suction elements 15 Liquid can be with the suction end of moistening suction elements 15 so that the suction end of suction elements 15 forms liquid film, and the liquid film can be When suction elements 15 continue to draw biometric print unit C, it is to avoid suction end and biometric print unit C directly contacts, such that it is able to The form and biological activity of biometric print unit C are prevented because suffering damage and destroying with suction end rigid contact.Also, due to Liquid film can separate suction end and biometric print unit C, even if particle diameter is less than the biometric print unit C of suction end suction port diameter Can also be maintained at suction port in the presence of liquid film and negative pressure, therefore, can also effectively overcome non-by arranging liquid film Particle size requirements of the suction microgranule acquisition modes to biological print unit C, expand the applicable model of this non-suction microgranule acquisition modes Enclose so that more specifications and a greater variety of biometric print unit C can be steered on the premise of by compared with Small loss, and by In the biometric print unit C compared with small particle, the cell quantity included inside it is relatively fewer, relative for the demand of nutrition supply It is relatively low, therefore, realize obtaining the non-suction-type compared with small particle biometric print unit C using liquid film, it is also beneficial to printed The biological activity of biometric print unit C is preferably maintained in journey, and contributes to finally giving the biology with more preferable biological activity Construct.

Additionally, the liquid film for being formed in suction end in advance, attraction can be played to the biometric print unit C of rear absorption and is made With so that biometric print unit C is not only subject to suction function, the surface tension effects of liquid film is also subject to simultaneously, on the one hand, this can The negative pressure applied needed for reduce suction elements 15, can not only reduce aspiration technique requirement, improve and draw chance of success, moreover it is possible to Enough mitigate damage of the suction force to biological print unit C；On the other hand, this can also make biometric print unit C by more firmly It is maintained at the suction port of suction end, improves the fastness of suction process, and causes biometric print unit C in the process being shifted In can be securely held at muzzle, be difficult to drop, improve shifting process stability and reliability；Another further aspect, liquid Film is in all the time liquid environment during being sucked out can biometric print unit C until being placed on setting print position In, and liquid environment is the key factor that biometric print unit C keeps biological activity, therefore, by arranging liquid film, can be with Even if so that biometric print unit C remains on the activity that can keep good in shifting process, improving the success of biometric print Rate.

In order to coordinate suction elements 15 only to draw single biometric print unit C to realize high-precision printing every time, at one In preferred embodiment, with reference to shown in Fig. 5, Fig. 9 and Figure 10, biological construct printing equipment is also included for holding biometric print Multiple hole positions 24 are provided with the print unit test kit 4 of unit C, print unit test kit 4, each hole position 24 is used to deposit single Biometric print unit C.

In order to realize the accurate seizure to biological print unit C in order to be accurately positioned to suction elements 15, such as tie Close shown in Fig. 1, Fig. 7 and Figure 10, biological construct printing equipment preferably includes controlling organization and for print unit Biometric print unit C in test kit 4 carries out the visual system 14 of position capture, and visual system 14 can capture single life Feedback signal is sent to controlling organization after thing print unit C, controlling organization controls suction elements 15 and is moved to according to feedback signal Corresponding position and biometric print unit C is drawn in print unit test kit 4, suction elements 15 are controlled then and is moved to adhesive layer Biometric print unit C is discharged at B location.Wherein visual system 14 is preferably made up of CCD camera, is readily able to realize to biology The accurate seizure of positions of the print unit C in print unit test kit 4, feeds back to controlling organization by position signalling then, real Existing automatization is digital control, is conducive to improving printing efficiency.

As the improvement to above-described embodiment, as shown in figure 8, biological construct printing equipment also includes the 3rd application member 20, in biological construct surface spraying moulding material or backing material, by arranging the 3rd application member 20, printing shaping Biological construct surface on be coated with moulding material or backing material, for protecting or supporting the biological of printing shaping to build Body.

In the above-described embodiments, biological construct can be non-lumen organization's structure, and now holder is planar substrates； Can advantageously be lumen organization's structure, now holder 29 is preferably swingle, predeterminable area is the outer wall of swingle.This Utility model biological construct printing equipment is particularly suited for printing lumen organization's structure, and specifically lumen organization's structure is blood vessel Precursor, moulding material or backing material include biocompatible materialses.As shown in Figure 3 and Figure 4, using this utility model biology structure In building the print procedure of body printing equipment, for the priority arrangement mode of biometric print unit C, can select as shown in Figure 3 The arrangement mode of " linear array after first circumference ", it is also possible to select the arrangement side of " circumference array after first straight line " as shown in Figure 4 Formula.

Below with lumen organization's structure be blood vessel precursor, holder 29 as swingle as a example by combine Fig. 5～Figure 11 shown in Embodiment is as follows come the print procedure for illustrating this utility model biological construct printing equipment：

As shown in figure 5, the agent structure of biological construct printing equipment includes that pedestal 1, the first nozzle component 2, second spray Head assembly 3, print unit test kit 4, the raw instrument 5 of rotation and electric cabinet 6, in electric cabinet 6 controlling organization is provided with.As shown in fig. 6, Column 7 is provided with pedestal, column 7 is provided with grating scale 8 and guide rail 10, linear electric motors 9 and respectively to the and of the first nozzle component 2 Second nozzle component 3 carries out the first Z-direction motion module 12 and the second Z-direction motion module 11 of Z-direction elevating control.

As shown in fig. 7, the first nozzle component 2 is main by the first fixed plate 13, visual system 14, suction elements 15, second Application member 16 (preferably shower nozzle), the first driving drive cylinder 18 of guide rail 17 and first are constituted, visual system 14, drawing section Part 15, second application member the 16, first driving drive cylinder 18 of guide rail 17 and first are arranged on vertical by the first fixed plate 13 The side of post 7.As shown in figure 8, the second nozzle component 3 is main by the second fixed board 19, the 3rd the 20, first coating of application member Part 21, second drives the drive cylinder 23 of guide rail 22 and second to constitute, the 3rd application member 20, the first application member 21, the The drive cylinder 23 of two driving guide rail 22 and second is arranged on the opposite side of column 7 by the second fixed board 19.

As shown in Figure 9 and Figure 10, the main orifice plate being located at by orifice plate support in orifice plate support 25 of print unit test kit 4, Multiple hole positions 24, perspective are formed with orifice plate and feel that light source 26 and the 3rd drives guide rail 27 to constitute.Perspective feels the effect of light source 26 Visual light is to provide so that visual system 14 is more easily detected biometric print unit C in the presence of light source.Such as Figure 11 institutes Show, the raw instrument 5 of rotation is main by bearing 28, electric rotating machine 30, holder 29 (swingle) and to be arranged on the scraper of the both sides of bearing 28 Rope 31 is constituted, and electric rotating machine 30 is used to drive the rotation of swingle consequently facilitating adjustment print position, and scraper rope 31 is used for the One application member 21, the second application member 16 and the 3rd application member 20 spray unnecessary feed liquid and carry out scraped finish, it is to avoid produce Blocking.

In print procedure, first, the first nozzle component 2 is moved to into safe distance along Z axis, make the second application member 16 Any object to pedestal 1 will not be hit when putting down.Then in the presence of the first drive cylinder 18, the edge of the second application member 16 First driving guide rail 17 goes downwards to spacing bottom.First nozzle component 2 makees X to fortune in the presence of linear electric motors 9 along guide rail 10 Move to the print range of holder 29.First nozzle component 2 under the control of the first Z-direction motion module 12, along Z axis to down to Height is printed, the second application member 16 sprays as illustrated in fig. 1 spacer medium (temperature-sensitive hydrogel) and forms spacer medium layer A. After having sprayed, the second application member 16 goes upward to upper limit root in the presence of the first drive cylinder 18 along the first driving guide rail 17 Portion, the first nozzle component 2 returns to leftward position.

Secondly, the second nozzle component 3 is moved to into safe distance along Z axis, the first application member 21 is not hit when putting down To following any object, then in the presence of the second drive cylinder 23, the first application member 21 drives guide rail 22 along second Go downwards to spacing bottom.Second nozzle component 3 makees X to moving to holder 29 in the presence of linear electric motors 9 along guide rail 10 Print range, under the control of the second Z-direction motion module 11, along Z-direction down to height is printed, first applies the second nozzle component 3 Cover part 21 and spray the first reagent to form adhesive layer B as shown in Figure 1.After having sprayed, the first application member 21 drives second Take offence in the presence of cylinder 23, along the second driving guide rail 22 upper limit root is gone upward to, the second nozzle component 3 returns to right positions.

After swingle has sprayed materials at two layers, will carry out bonding the work of biometric print unit C.First it is to adopt vision 14 couples of biology print unit C of system are positioned.Because object of the visual system 14 not to stacking is identified, it is therefore desirable to Each biometric print unit C is placed in single hole position 24, in case forming stacking.This is when prepared by biometric print unit C Time is just ready for, it is ensured that the inner only one of which biometric print unit C of each hole position 24.Now only need to be by visual system 14 Camera lens center is directed at the circle scope in hole position 24, within the range only one of which biometric print unit C, therefore soon Biometric print unit C is can recognize that, and is accurately positioned.It is embodied as follows：After program setting, by the phase in visual system 14 First, upper left corner hole position 24 in the center alignment orifice plate of machine camera lens, Y is by motor 26 along the 3rd driving guide rail 27 To motion, the first nozzle component 2 is X to motion to realize.Z-direction motion is done by the first nozzle component 2, camera lens top is made Object keeps default distance with being captured, and now, biometric print unit C is placed exactly in the range of camera focus, and the scope Interior only one of which biometric print unit C, it is thereby achieved that the quick seizure positioning of biometric print unit C.

Behind the center for obtaining the first nozzle component 2, controlling organization is conversed and X, Y of suction elements 15 automatically Coordinate figure.In the presence of controlling organization, Y-direction done along the 3rd driving guide rail 27 by motor 26 move and do Y-direction and move, First nozzle component 2 is X to motion, the biometric print unit C centers for catching the alignment of suction elements 15.Meanwhile, the first shower nozzle Component 2 does Z axis downward movement to after setpoint distance, as shown in Fig. 2 suction elements 15 open air suction function, holds biometric print Unit C so as to do not drop.First nozzle component 2 goes upward to safe distance along Z-direction, then need to put to holder 29 is moved to along X The position of biometric print unit C, the first nozzle component 2 moves downward row to program setting height along Z axis, and suction elements 15 are automatic Blowing function is opened, biometric print unit is put on holder 29, first biometric print unit is put and finished.Repetition Front action, it is different from what arrangement was required according to biometric print unit C when second biometric print unit C is put, as The swingle of holder 29 will automatically move corresponding distance, to realize the exact placement on swingle.By that analogy, so as to reality Duplicate printing shown in existing Fig. 3 and Fig. 4, until biometric print unit C is paved with swingle surface, terminates biometric print unit C Put, the first nozzle component 2 returns to leftward position.

Finally, mobile second nozzle component 3, by the 3rd application member 20 print position is moved to, spray moulding material or Backing material (such as outermost biocompatible materialses), print procedure is finished.

Above in association with embodiment embodiment of the present utility model is described in detail, but this utility model not office It is limited to described embodiment.For a person skilled in the art, without departing from principle of the present utility model and essence Various changes, modification, equivalence replacement and modification are carried out in the case of spirit to these embodiments to still fall within this utility model Protection domain within.

Claims (11)

1. a kind of biological construct printing equipment, it is characterised in that include：Holder (29), the first application member (21), reagent Feedway and placement mechanism, wherein,

First application member (21) is viscous to be formed for the predeterminable area that the first reagent is coated in the holder (29) Close layer (B)；

The reagent feedway is used to supply first reagent to first application member (21)；And

The placement mechanism is used to for the biometric print unit (C) to be placed on the adhesive layer (B), so that the biology is beaten Impression unit (C) is bonded on the adhesive layer (B) so that at least form a part for biological construct.

2. biological construct printing equipment according to claim 1, it is characterised in that also including the second application member (16), second application member (16) in the predeterminable area of the holder (29) for coating in advance spacer medium to be formed Spacer medium layer (A), the adhesive layer (B) is coated on the holder (29) by the spacer medium layer (A).

3. biological construct printing equipment according to claim 1, it is characterised in that the placement mechanism includes drawing section Part (15), the suction elements (15) are for drawing the biometric print unit (C) and be moved at the adhesive layer (B) position The biometric print unit (C) is discharged, to realize that the biometric print unit (C) is bonded on the adhesive layer (B).

4. biological construct printing equipment according to claim 3, it is characterised in that the suction elements (15) can be pre- First draw liquid and make the suction end of the suction elements (15) form liquid film, the biometric print unit (C) is by the absorption Part (15) is maintained at the suction end of the suction elements (15) and contacts with the liquid film after drawing.

5. biological construct printing equipment according to claim 3, it is characterised in that the suction elements (15) include to Few absorption position, the single biometric print unit (C) is only drawn every time at each described absorption position.

6. biological construct printing equipment according to claim 3, it is characterised in that also include for holding the biology Multiple hole positions (24) are provided with the print unit test kit (4) of print unit (C), the print unit test kit (4), each institute Hole position (24) is stated for depositing the single biometric print unit (C).

7. biological construct printing equipment according to claim 5, it is characterised in that the suction elements (15) are including number The individual absorption position, several drawing section potential energies it is enough by the several described biometric print unit (C) drawn and meanwhile discharge to The adhesive layer (B).

8. biological construct printing equipment according to claim 6, it is characterised in that also include：Controlling organization；Vision system System (14), for carrying out position capture to the biometric print unit (C) in the print unit test kit (4)；

Wherein described visual system (14) is for the single biometric print unit (C) is being captured, backward the controlling organization to be sent out Feedback signal, the controlling organization is sent to control the suction elements (15) according to the feedback signal and be moved to the print unit Corresponding position and the biometric print unit (C) is drawn in test kit (4), the suction elements (15) are controlled then and is moved to The biometric print unit (C) is discharged at adhesive layer (B) position.

9. biological construct printing equipment according to claim 1, it is characterised in that also including the 3rd application member (20), for spray mo(u)lding material or backing material on the surface of biological construct.

10. biological construct printing equipment according to claim 1, it is characterised in that the biological construct is tube chamber Organizational structure, the holder (29) is swingle, and the predeterminable area is the outer wall of the swingle.

11. biological construct printing equipments according to claim 10, it is characterised in that lumen organization's structure is blood Pipe precursor.