CN110192495A - A kind of methods and applications using tobacco rod preparation oil tea mushroom culture medium - Google Patents
A kind of methods and applications using tobacco rod preparation oil tea mushroom culture medium Download PDFInfo
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- CN110192495A CN110192495A CN201910568802.5A CN201910568802A CN110192495A CN 110192495 A CN110192495 A CN 110192495A CN 201910568802 A CN201910568802 A CN 201910568802A CN 110192495 A CN110192495 A CN 110192495A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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Abstract
The invention belongs to technical field of edible fungi cultivation, tobacco rod sunning, crushing are first banked up fermentation and tobacco rod fermentation material are made by especially a kind of methods and applications using tobacco rod preparation oil tea mushroom culture medium after mixing with EM biofermentation bacterium solution, tea polyphenols solution；Then cotton seed hulls 30-50%, wheat bran 15-25%, tobacco rod fermentation material 15-35%, bagasse 5-15%, dregs of beans 1-5%, king's rice flour 3-8%, gypsum 1-5% are configured to culture medium by weight.The culture medium stick is inoculated with tree fungus, after cultural hypha, moves into greenhouse fruiting.Culture medium prepared by the present invention cultivates oil tea mushroom, compared with traditional sawdust medium, the cultural hypha time shortens 15 days, and whole mycelia is more pure white dense, and fruiting time is more concentrated, change of tide time highest can shorten 4-5 days, bacterial contamination rate reduces 5-8%, and cost of material saves 15-20%, and biological transformation ratio improves 20-21%, average product increases 12-13%, and overall yield is more stable.
Description
Technical field
The invention belongs to technical field of edible fungi cultivation, especially a kind of method using tobacco rod preparation oil tea mushroom culture medium
And application.
Background technique
Oil tea mushroom also known as agrocybe, Agrocybe aegerita, Agrocybe chaxingu etc. are a kind of edible and medical fungis, and cap is delicate, handle is crisp, taste is pure
It is fragrant, tasty, it gains the name because wild in dried-up go up of tea oil tree.Oil tea mushroom is full of nutrition, 18 containing needed by human body kind ammonia
8 kinds of amino acid that base acid, especially human body itself cannot synthesize, also containing glucose, mycoprotein, carbohydrate, the dimension life of B race
The nutritional ingredients such as element and iron, potassium, zinc, selenium；With kidney tonifying, diuresis, soreness of waist pain, excreting dampness, invigorating the spleen, antidiarrheal and other effects are controlled, is a kind of
The healthy food extensively to get consumer reception.
Currently, oil tea mushroom has realized that factory culture, culture medium generally use the timber such as sawdust as primary raw material.With
The production-scale continuous expansion of oil tea mushroom, the consumption of timber steeply rises, and the limited source of timber, increases forestry money
The production cost in source, the pressure of ecological environment and oil tea mushroom.Therefore, the substance that research cost is cheap, easily obtains replaces culture medium
In sawdust become the urgent need of oil tea mushroom industry development.
Tobacco is Solanaceae grass platymiscium, originates in South America, and China introduces tobacco very early, cultivated area and cigarette output
It is at the forefront in the world.According to ASSOCIATE STATISTICS, China about generates the tobacco industries waste such as 901,500,000 tons of tobacco rods and offal every year.This
A little wastes are both not suitable for making fuel, are also not suitable for returning to the field and make fertilizer, be largely dropped or burn, not only cause serious environment
Pollution and the huge wasting of resources.How these waste resources are recycled, it has also become tobacco business is badly in need of the class researched and solved
Topic.Currently, utilizing mainly to Comprehensive Utilization of Tobacco Waste has: manufacturing particieboard, active carbon using tobacco rod, utilize tobacco waste
Extraction nicotine, niacin；But these resource utilization methods, there are the jejune problem of at high cost or technology, also non-industrialization pushes away
Extensively.Cellulose and content of lignin are high in tobacco rod, are good containing nutrients such as carbohydrate, protein, nitrogen, phosphorus, potassium and microelements
Edible fungi material, be applied to culture medium for cultivating edible mushroom have very high utility value；The country, which has had, utilizes tobacco rod portion
Divide the report of substitution major ingredient culturing edible fungus.A kind of utilization as disclosed in the file that number of patent application is CN201710468685.6
The culture medium prescription and preparation method and application of tobacco rod culturing edible fungus are added 0.1% ammonium hydroxide and soak and build heap after crushing tobacco rod
After fermentation tobacco rod is made in fermentation, it is mixed and made into the culture medium of culturing edible fungus with thin sawdust, wheat bran, land plaster, is applied to hedgehog hydnum
The cultivation of mushroom, oyster mushroom.For another example number of patent application is a kind of base of culturing edible fungus disclosed in the file of CN201410154515.7
The preparation method of matter, after flue-cured tobacco bar, mulberry branch, pine sawdust are crushed, with wheat bran or husk, glucose, gypsum, cassava alcohol slag
And processed walnut shell is mixed and made into culture medium.Both it is directly to be utilized after crushing tobacco rod, or utilized after fermentation, does not have
Have and the harmful substances such as nicotine in tobacco rod are handled, nicotine content is high in tobacco rod, and average content about 1.5-2.0%, has
Up to 3.5-4.5%, it is unfavorable to mycelia growth and fruit-body formation as culture medium for cultivating edible mushroom raw material；And these
The effect of culture medium cultivation oil tea mushroom also requires study.
Summary of the invention
In order to solve the above-mentioned problems in the prior art, researcher of the present invention to the processing method of nicotine in tobacco rod into
It has gone research, has provided a kind of methods and applications using tobacco rod preparation oil tea mushroom culture medium, solve current tobacco waste and exist
Disposal of pollutants problem during production of cigarettes, turns waste into wealth, while also solving current China oil tea mushroom industry and being badly in need of a large amount of wood
The problem of matter cultivation matrix；It is realized particular by following technical scheme:
A method of oil tea mushroom culture medium is prepared using tobacco rod, which is characterized in that it is broken that discarded tobacco rod is ground into tobacco rod
After slag with EM biofermentation bacterium solution, tea polyphenols solution mix, bank up fermentation and tobacco rod fermentation material be made, using tobacco rod fermentation material as
The raw material of oil tea mushroom culture medium.
Preferably, the method using tobacco rod preparation oil tea mushroom culture medium, specifically includes the following steps:
(1) pretreatment of raw material: tobacco rod natural drying is done, and crushed 10-30 mesh screen；
(2) tobacco rod ferments: EM biofermentation bacterium solution being added into smashed tobacco rod disintegrating slag, then on tobacco rod disintegrating slag surface
Tea polyphenols solution is sprayed, after stirring, fermentation is banked up, obtains tobacco rod fermentation material；
(3) culture medium is prepared: by tobacco rod fermentation material and cotton seed hulls, wheat bran, bagasse, dregs of beans, king's rice flour, gypsum by certain
Ratio be configured to mixture, adjust its water content after 60-70%, adjust pH value be 5-7 to get oil tea mushroom culture medium.
Preferably, the additional amount of the EM biofermentation bacterium solution is the 0.5-0.8% of tobacco rod disintegrating slag weight, tea polyphenols solution
Additional amount be tobacco rod disintegrating slag weight 10-15%.
Preferably, the mass concentration of the tea polyphenols solution is 20%.
Preferably, the way to manage for banking up fermentation are as follows: material heap temperature is often increased to 60-65 DEG C of turning one during fermentation
Secondary, three times, until tobacco rod disintegrating slag darkens, quality softens for turning altogether.
Preferably, the mixture is by weight percentage by 30-50% cotton seed hulls, 15-25% wheat bran, 15-35% cigarette
Bar fermentation material, 5-15% bagasse, 1-5% dregs of beans, 3-8% king's rice flour, 1-5% gypsum are configured to.
The present invention also provides the applications of above-mentioned oil tea mushroom culture medium, including following procedure:
(1) the oil tea mushroom culture medium prepared is used into long 350mm × wide 170mm × thickness 0.05mm high pressure polyethylene bag
Son pack, through 120 DEG C high pressure sterilization 2-4 hours, be cooled to room temperature, obtain bacteria stick；
(2) bacteria stick is inoculated with tree fungus in an aseptic environment, the bacteria stick after inoculation is cultivated into mycelia in constant temperature incubation room
22-28 days, after bacteria stick covers with mycelia, move into fruiting greenhouse fruiting.
Preferably, the specification of the tree fungus are as follows: long 12cm × wide 0.3cm × high 0.6cm, each bacteria stick are inoculated with 1.
Preferably, the temperature of the constant temperature incubation room is 25 ± 1 DEG C, humidity 70%-80%.
The present invention also studies the fermentation processing method of discarded tobacco rod:
1, EM biofermentation bacterium solution adds quantity research: the tobacco rod after will be discarded is ground into the tobacco rod disintegrating slag of 20 mesh, and addition is not
The EM biofermentation bacterium solution of same amount, banks up fermentation and tobacco rod fermentation material is made after mixing.It is banked up with not adding EM biofermentation bacterium solution
Fermentation is used as control experiment group, detects the ingredient of different lower the obtained tobacco rod fermentation materials of EM biofermentation bacterium solution additive amount, detects
The results are shown in Table 1:
Influence of the table 1EM biofermentation bacterium solution additive amount to tobacco rod fermentation material ingredient
As shown in Table 1, addition EM biofermentation bacterium solution can obviously increase protein content, soluble carbon water in fermentation material
Compounds content reduces Fiber and lignin concentration, and with the increase of additive amount, protein content, soluble carbon aquation
Close object content and increase more, Fiber and lignin concentration decline is lower, but more than 0.5% after, as additive amount increases, ferment
Each composition transfer is little in material, tends to be steady substantially, considers production cost factor, so selection EM biofermentation bacterium solution additive amount
For 0.5-0.8%.
2, tea polyphenols solution adds quantity research: the tobacco rod after discarding is ground into the tobacco rod disintegrating slag of 20 mesh, adds not same amount
Tea polyphenols solution, the mass concentration of tea polyphenols solution is 20%, fermentation is banked up after mixing, tobacco rod fermentation material is made.Simultaneously with not
Addition tea polyphenols solution banks up fermentation as control experiment group, detects different lower the obtained tobacco rod fermentations of tea polyphenols solution additive amount
Nicotine content in material, testing result are as shown in table 2:
Influence of the 2 tea polyphenols solution additive amount of table to nicotine content in tobacco rod fermentation material
As shown in Table 2, addition tea polyphenols solution can reduce the nicotine content in tobacco rod fermentation material, additive amount be 10% with
On, nicotine content reduces obvious, but additive amount is more than after 10%, and nicotine content variation tends to be steady substantially, consideration production cost,
The factors such as operation difficulty select tea polyphenols solution additive amount for 10-15%.
3, tobacco rod grinding particle size is studied: tobacco rod being ground into the tobacco rod disintegrating slag of different meshes, the EM of its weight 0.5% is added
Biofermentation bacterium solution and 10% mass concentration be 20% tea polyphenols solution, fermentation is banked up after mixing, tobacco rod fermentation material is made,
The ingredient of tobacco rod fermentation material under different meshes is detected, testing result is as shown in table 3:
The ingredient of 3 different meshes tobacco rod fermentation material of table
As shown in Table 3, as tobacco rod crushes the increase of mesh number, protein content, water-soluble carbohydrate content increase
More, cellulose, lignin, nicotine content reduce faster, but more than 10 mesh after, as mesh number increases, each ingredient in fermentation material
Variation less, tends to be steady substantially, the factors such as production cost, operation difficulty is considered, so selecting smashing fineness for 10~30 mesh.
The beneficial effects of the present invention are:
1, using fermentation material made of the fermentation of tobacco waste tobacco rod, it is main as oil tea mushroom culture medium for cultivating that sawdust can be substituted
Raw material has not only saved timber resources, but also realizes the resource utilization of tobacco waste tobacco rod, solves current tobacco waste in cigarette
Disposal of pollutants problem in production process, turns waste into wealth；
2, EM biofermentation bacterium solution and tea polyphenols solution mixed fermentation tobacco rod waste residue are added, the decomposed of tobacco rod can be accelerated, had
Conducive to tobacco rod fermentation, accelerates crude fibre decomposition and be converted into small molecule structure, be easy to later period oil tea mushroom strains and absorb nutriment；Simultaneously again
It can reduce the nicotine in tobacco rod, reduce nicotine to the adverse effect of the growth of oil tea mushroom silk and fruit-body formation；
3, compared to traditional sawdust medium, oil tea mushroom, cultural hypha time are cultivated using culture medium prepared by the present invention
It shortens 15 days, and whole mycelia is more pure white dense, fruiting time is more concentrated, and change of tide time highest can shorten 4-5 days, miscellaneous
Bacterium pollution rate reduces 5-8%, and cost of material saves 15-20%, and biological transformation ratio improves 20-21%, and average product increases 12-
13%, overall yield is more stable.
Feasible process degree provided by the invention is high, easy to operate, obvious technical effects, has very high economic benefit and society
It can benefit.
Specific embodiment
It is limited below with reference to specific embodiment technical solution of the present invention is further, but claimed
Range is not only limited to made description.
Embodiment 1
The preparation of oil tea mushroom culture medium:
(1) pretreatment of raw material: tobacco rod natural drying is done, and crushed 10 mesh screens；
(2) tobacco rod ferments: 0.5% EM biofermentation bacterium solution of weight is added after to above-mentioned crushing in tobacco rod disintegrating slag, so
The tea polyphenols solution for being afterwards 20% in 10% mass concentration of tobacco rod disintegrating slag surface sprinkling tobacco rod disintegrating slag weight；It stirs evenly
Afterwards, bank up fermentation, it is primary to be often increased to 60-65 DEG C of turning for material heap temperature during fermentation, altogether turning three times, until tobacco rod disintegrating slag color
It deepens, quality softens to get tobacco rod fermentation material；
(3) culture medium prepare: according to weight ratio cotton seed hulls 50%, wheat bran 15%, tobacco rod fermentation material 20%, bagasse 5%,
Dregs of beans 5%, king's rice flour 3%, 2% ingredient of gypsum, stir evenly, and 60%, adjusting pH value with limewash is 5 for water content control,
Up to oil tea mushroom culture medium.
The application of oil tea mushroom culture medium
(1) above-mentioned prepared oil tea mushroom culture medium is taken, it is poly- using long 350mm × wide 170mm × thickness 0.05mm high pressure
Ethylene sack pack, through 120 DEG C high pressure sterilization 2 hours, be cooled to room temperature, be made bacteria stick；
(2) bacteria stick is inoculated with tree fungus, the specification of tree fungus in an aseptic environment are as follows: long 12cm × wide 0.3cm ×
High 0.6cm, every root fungus stick are inoculated with 1；Bacteria stick after inoculation is placed on to the thermostatic chamber of 25 ± 1 DEG C of temperature, humidity 70%-80%
Middle culture mycelia 22 days, after bacteria stick covers with mycelia, move into fruiting greenhouse fruiting.
Embodiment 2
The preparation of oil tea mushroom culture medium:
(1) pretreatment of raw material: tobacco rod natural drying is done, and crushed 20 mesh screens；
(2) tobacco rod ferments: 0.6% EM biofermentation bacterium solution of weight is added after to above-mentioned crushing in tobacco rod disintegrating slag, so
The tea polyphenols solution for being afterwards 20% in the mass concentration of tobacco rod disintegrating slag surface sprinkling tobacco rod disintegrating slag weight 12%；It stirs
Afterwards, bank up fermentation, it is primary to be often increased to 60-65 DEG C of turning for material heap temperature during fermentation, altogether turning three times, until tobacco rod disintegrating slag color
It deepens, quality softens to get tobacco rod fermentation material；
(3) culture medium is prepared: according to weight ratio cotton seed hulls 40%, wheat bran 20%, tobacco rod fermentation material 15%, bagasse
10%, dregs of beans 4%, king's rice flour 8%, 3% ingredient of gypsum, stir evenly, and water content control adjusts pH value 65%, with limewash
For 6 to get oil tea mushroom culture medium.
The application of oil tea mushroom culture medium:
(1) above-mentioned prepared culture medium is taken, using long 350mm × wide 170mm × thickness 0.05mm high pressure polyethylene bag
Son pack, through 120 DEG C high pressure sterilization 3 hours, be cooled to room temperature, obtain bacteria stick；
(2) bacteria stick is inoculated with tree fungus, the specification of tree fungus in an aseptic environment are as follows: long 12cm × wide 0.3cm ×
High 0.6cm, every root fungus stick are inoculated with 1；Then the bacteria stick after inoculation is placed on 25 ± 1 DEG C of temperature, the perseverance of humidity 70%-80%
Hot-house culture mycelia 25 days, after bacteria stick covers with mycelia, move into fruiting greenhouse fruiting.
Embodiment 3
The preparation of oil tea mushroom culture medium
(1) pretreatment of raw material: tobacco rod natural drying is done, and crushed 30 mesh screens；
(2) tobacco rod ferments: the EM biofermentation bacterium solution of its weight 0.8% is added after to above-mentioned crushing in tobacco rod disintegrating slag；So
The tea polyphenols solution for being afterwards 20% in the mass concentration of tobacco rod disintegrating slag surface sprinkling tobacco rod disintegrating slag weight 15%；It stirs
Afterwards, bank up fermentation, it is primary to be often increased to 60-65 DEG C of turning for material heap temperature during fermentation, altogether turning three times, until tobacco rod disintegrating slag color
It deepens, quality softens to get tobacco rod fermentation material；
(3) culture medium prepare: according to weight ratio cotton seed hulls 30%, wheat bran 25%, tobacco rod fermentation material 35%, bagasse 5%,
Dregs of beans 1%, king's rice flour 3%, 1% ingredient of gypsum, stir evenly, and 70%, adjusting pH value with limewash is 7 for water content control,
Up to oil tea mushroom culture medium.
The application of oil tea mushroom culture medium:
(1) above-mentioned prepared culture medium is taken, using long 350mm × wide 170mm × thickness 0.05mm high pressure polyethylene bag
Son pack, through 120 DEG C high pressure sterilization 4 hours, be cooled to room temperature to get bacteria stick；
(2) it is inoculated with: bacteria stick being inoculated with tree fungus, the specification of tree fungus in an aseptic environment are as follows: long 12cm × wide
0.3cm × high 0.6cm, every root fungus stick are inoculated with 1；Bacteria stick after inoculation is placed on 25 ± 1 DEG C of temperature, humidity 70%-80%
Constant temperature incubation room is cultivated mycelia 28 days, after bacteria stick covers with mycelia, moves into fruiting greenhouse fruiting.
Control group 1
Traditional sawdust medium formula 1: by weight cotton seed hulls 47%, wheat bran 20%, dregs of beans 10%, sawdust 20%, stone
Grey 2%, 1% ingredient of gypsum, stir evenly, and 60~70%, adjusting pH value with limewash is 5~7 for water content control, stick processed,
It is inoculated with solid spawn, cultural hypha, fruiting step are identical with the embodiment of the present invention.
Control group 2
Traditional sawdust medium formula 2: by weight cottonseed Sales 42%, wheat bran 20%, sawdust 35%, lime 2%, gypsum
1% ingredient, stirs evenly, and 60~70%, adjusting pH value with limewash is 5~7 for water content control, stick processed, inoculation solid bacterium
Kind, cultural hypha, fruiting step are identical with the embodiment of the present invention.
Control group 3
A kind of preparation method of the matrix of culturing edible fungus disclosed in the file of CN201410154515.7:
Walnut shell is handled, it is 10mm particle below that walnut shell powder, which is broken into diameter, impregnates core in vegetation buck
Peach shell 24-48 hours, the quality of vegetation buck was 2-6 times of walnut shell quality, stand-by after processing；
Mulberry branch, flue-cured tobacco bar and pine sawdust are handled, pine sawdust is ground into 10mm particle below, by mulberry branch, is baked
It is diameter 15mm graininess below that tobacco rod, which smashes, and mulberry branch, flue-cured tobacco bar and particles of pine are taken off dilute terpene, ungrease treatment, to
With；
By weight, it by 30 parts of walnut shell, 10 parts of mulberry branch, 10 parts of pine sawdust, 5 parts of flue-cured tobacco bar, 10 parts of husk, is added
0.5 part of glucose and 1 part of gypsum are mixed and made into matrix.
Take the substrate culture oil tea mushroom:
Stick processed, inoculation solid spawn, cultural hypha, fruiting step are identical with the embodiment of the present invention.
Control group 4
Number of patent application is a kind of culture using tobacco rod culturing edible fungus disclosed in the file of CN201710468685.6
Based formulas and preparation method and application:
A kind of culture medium prescription using tobacco rod culturing edible fungus, is made of following components in percentage by weight: fermentation cigarette
Bar 65%, jig saw wood 15%, wheat bran 19%, land plaster 1%.This formula is culture medium for cultivating " Pinggu " mushroom formula.The jig saw wood
Grain diameter≤3mm, land plaster are 60 mesh.
Utilize the preparation method of the culture medium prescription of tobacco rod culturing edible fungus, comprising the following steps:
Step 1, tobacco rod pretreatment of raw material: is ground into the tobacco rod particle of 0.6 × 0.6 × 0.6cm or so；Using volume
0.1% ammonium hydroxide of score soaks tobacco rod particle, soaks rear tobacco rod water content 60%, and composting carries out turning afterwards for 24 hours, according to material heap moisture
Lost situation, moisturizing is primary, and during which maintaining the water content of tobacco rod is 55-60%；Continuing fermentation time 8-9d, (period keeps environment
Middle relative humidity is 45-60%), it during which waits for that material heap central temperature reaches 80 DEG C of then turnings, obtains fermentation tobacco rod；Wherein, volume
0.1% ammonium hydroxide of score is diluted made of 100 times 10% ammonium hydroxide of volume fraction；
Step 2, each component ingredient: is weighed by following weight percent: fermentation tobacco rod 65%, jig saw wood 15%, wheat bran
19%, land plaster 1% is uniformly mixed so as to obtain mixed material；
Step 3, spice: the culture of culturing edible fungus is obtained to mixed material plus water until moisture content reaches 65%
Base.
Take the substrate culture oil tea mushroom:
Stick processed, inoculation solid spawn, cultural hypha, fruiting step are identical with the embodiment of the present invention.
The results are shown in Table 4 for Statistics Implementation example and control group 1-4 different culture medium cultivation oil tea mushroom:
4 different culture medium of table cultivates oil tea mushroom Comparative result
As can be seen from Table 4, oil tea mushroom is cultivated using culture medium prepared by the present invention, with traditional sawdust medium and
Invention disclosed Patent media formula is compared, and the cultural hypha time shortens 15 days or so, and whole mycelia is more pure white dense
Close, fruiting time is more concentrated, and change of tide time highest can shorten 4-5 days, and bacterial contamination rate reduces 5-8%, cost of material saving
15-20%, biological transformation ratio improve 20-21%, and average product increases 12-13%, and overall yield is more stable；Technical effect is aobvious
It writes, high financial profit.
It is important to point out that, above embodiments and test example are only limitted to do further technical solution of the present invention herein
Elaboration and understanding, should not be understood as it is further to technical solution of the present invention limited, what those skilled in the art made
The innovation and creation of non-protruding essential characteristics and marked improvement still fall within protection category of the invention.
Claims (10)
1. a kind of method using tobacco rod preparation oil tea mushroom culture medium, which is characterized in that biological with EM after crushing discarded tobacco rod
Zymocyte liquid, tea polyphenols solution mix, and bank up fermentation and tobacco rod fermentation material is made, using tobacco rod fermentation material as oil tea mushroom culture medium
Primary raw material.
2. utilizing the method for tobacco rod preparation oil tea mushroom culture medium as described in claim 1, which is characterized in that specifically include following
Step:
(1) pretreatment of raw material: tobacco rod natural drying is done, is pulverized and sieved；
(2) tobacco rod ferments: EM biofermentation bacterium solution being added into smashed tobacco rod disintegrating slag, then sprays on tobacco rod disintegrating slag surface
Tea polyphenols solution after stirring, banks up fermentation, obtains tobacco rod fermentation material；
(3) culture medium is prepared: tobacco rod fermentation material and cotton seed hulls, wheat bran, bagasse, dregs of beans, king's rice flour, gypsum are pressed certain ratio
Example is configured to mixture, adjusts its water content after 60-70%, and adjusting pH value is 5-7 to get oil tea mushroom culture medium.
3. utilizing the method for tobacco rod preparation oil tea mushroom culture medium as claimed in claim 1 or 2, which is characterized in that the EM is raw
The additional amount of object zymocyte liquid is the 0.5-0.8% of tobacco rod disintegrating slag weight, and the additional amount of tea polyphenols solution is tobacco rod disintegrating slag weight
10-15%.
4. utilizing the method for tobacco rod preparation oil tea mushroom culture medium as claimed in claim 2, which is characterized in that the tobacco rod disintegrating slag
Granularity be 10-30 mesh.
5. the method as described in any one of claims 1-3 using tobacco rod preparation oil tea mushroom culture medium, which is characterized in that described
The mass concentration of tea polyphenols solution is 20%.
6. utilizing the method for tobacco rod preparation oil tea mushroom culture medium as claimed in claim 1 or 2, which is characterized in that described to bank up
The way to manage of fermentation are as follows: it is primary to be often increased to 60-65 DEG C of turning for material heap temperature during fermentation, altogether turning three times, until tobacco rod is broken
Slag darkens, and quality softens.
7. utilizing the method for tobacco rod preparation oil tea mushroom culture medium as claimed in claim 2, which is characterized in that the mixture is pressed
Weight percent meter is by 30-50% cotton seed hulls, 15-25% wheat bran, 15-35% tobacco rod fermentation material, 5-15% bagasse, 1-5%
Dregs of beans, 3-8% king's rice flour, 1-5% gypsum are configured to.
8. a kind of application of the described in any item oil tea mushroom culture mediums using tobacco rod preparation of such as claim 1-7, feature exist
In, including following procedure:
(1) the oil tea mushroom culture medium prepared is filled using long 350mm × wide 170mm × thickness 0.05mm high pressure polyethylene sack
Bag, through 120 DEG C high pressure sterilization 2-4 hours, be cooled to room temperature, obtain bacteria stick；
(2) bacteria stick is inoculated with tree fungus in an aseptic environment, the bacteria stick after inoculation is cultivated into mycelia 22-28 in constant temperature incubation room
It, after bacteria stick covers with mycelia, moves into fruiting greenhouse fruiting.
9. utilizing the application of the oil tea mushroom culture medium of tobacco rod preparation as claimed in claim 8, which is characterized in that the branch bacterium
The specification of kind are as follows: long 12cm × wide 0.3cm × high 0.6cm.
10. utilizing the application of the oil tea mushroom culture medium of tobacco rod preparation as claimed in claim 8, which is characterized in that the constant temperature
The temperature of culturing room is 25 ± 1 DEG C, humidity 70%-80%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042467A (en) * | 2020-09-23 | 2020-12-08 | 玉龙县珍稀菇农业开发有限公司 | Process flow for preparing natural selenium-rich black fungus |
| CN114766283A (en) * | 2022-04-27 | 2022-07-22 | 贵州大学 | Auricularia polytricha culture medium containing walnut sawdust and preparation method thereof |
| CN117099608A (en) * | 2023-09-12 | 2023-11-24 | 西昌学院 | Tobacco waste composting pretreatment method |
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| CN114766283A (en) * | 2022-04-27 | 2022-07-22 | 贵州大学 | Auricularia polytricha culture medium containing walnut sawdust and preparation method thereof |
| CN117099608A (en) * | 2023-09-12 | 2023-11-24 | 西昌学院 | Tobacco waste composting pretreatment method |
| CN117099608B (en) * | 2023-09-12 | 2024-06-14 | 西昌学院 | Tobacco waste composting pretreatment method |
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Application publication date: 20190903 |