CN110114472A - 将线性测序文库转换为环状测序文库的方法 - Google Patents
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Abstract
提供了将线性测序文库转换为环状测序文库的方法。其中，该方法包括以下步骤：利用正向接头引物和反向接头引物将线性测序文库进行PCR扩增，以便获得扩增产物；将所述扩增产物进行热变性处理，以便获得单链DNA混合物；将所述单链DNA混合物进行环化处理，以便获得环状DNA产物，所述环状DNA产物构成所述环状测序文库，其中，正向接头引物与所述线性测序文库的正向接头的至少部分序列互补配对，反向接头引物与所述线性测序文库的反向接头的至少部分序列互补配对，正向接头引物和所述反向接头引物的至少之一的5’端核苷酸经过磷酸化修饰。
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PCT国内申请，说明书已公开。
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- PCT国内申请，权利要求书已公开。
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| PCT/CN2016/111365 WO2018112806A1 (zh) | 2016-12-21 | 2016-12-21 | 将线性测序文库转换为环状测序文库的方法 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114540473A (zh) * | 2021-08-27 | 2022-05-27 | 四川大学华西第二医院 | 一种新型核酸测序*** |
| WO2022222101A1 (zh) * | 2021-04-22 | 2022-10-27 | 深圳华大生命科学研究院 | Rna测序文库的构建方法、测序方法及试剂盒 |
| WO2023082305A1 (zh) * | 2021-11-09 | 2023-05-19 | 纳昂达(南京)生物科技有限公司 | 兼容双测序平台的建库元件、试剂盒及建库方法 |
| WO2024119413A1 (zh) * | 2022-12-07 | 2024-06-13 | 深圳华大智造科技股份有限公司 | 基于一步法的双核酸纳米球制备方法 |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2020532976A (ja) * | 2017-09-14 | 2020-11-19 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 環状一本鎖ｄｎａライブラリーを生成するための新規な方法 |
| CN110760936B (zh) * | 2018-07-26 | 2023-04-28 | 深圳华大生命科学研究院 | 构建dna甲基化文库的方法及其应用 |
| SG11202105957YA (en) * | 2018-12-05 | 2021-07-29 | Egi Tech Shen Zhen Co Limited | Rolling circle amplification method, method for preparing sequencing library, and dna nanosphere prepared therefrom |
| EP3992303A4 (en) * | 2019-06-26 | 2023-03-15 | MGI Tech Co., Ltd. | METHOD FOR BUILDING A HIGH-THROUGH-THROUGH NESTING MULTIPLEX PCR SEQUENCING LIBRARY AND KIT |
| WO2021051378A1 (zh) * | 2019-09-20 | 2021-03-25 | 武汉华大医学检验所有限公司 | 测序文库的构建方法、测序方法及试剂盒和应用 |
| CN113046835A (zh) * | 2019-12-27 | 2021-06-29 | 深圳华大生命科学研究院 | 检测慢病毒***位点的测序文库构建方法和慢病毒***位点检测方法 |
| CN112592962B (zh) * | 2020-11-18 | 2022-11-25 | 华中农业大学 | 适用于高通量转录组空间位置信息的检测方法及其应用 |
| CN113862261B (zh) * | 2021-11-15 | 2022-11-15 | 纳昂达(南京)生物科技有限公司 | 一组应用于不同高通量测序平台文库的通用环化陪伴序列、其试剂盒以及环化方法 |
| WO2023137667A1 (zh) * | 2022-01-20 | 2023-07-27 | 深圳华大智造科技股份有限公司 | 一种接头及其在构建dnb文库中的应用 |
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| US20100028873A1 (en) * | 2006-03-14 | 2010-02-04 | Abdelmajid Belouchi | Methods and means for nucleic acid sequencing |
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- 2016-12-21 WO PCT/CN2016/111365 patent/WO2018112806A1/zh active Application Filing
- 2016-12-21 CN CN201680091797.8A patent/CN110114472A/zh active Pending
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022222101A1 (zh) * | 2021-04-22 | 2022-10-27 | 深圳华大生命科学研究院 | Rna测序文库的构建方法、测序方法及试剂盒 |
| CN114540473A (zh) * | 2021-08-27 | 2022-05-27 | 四川大学华西第二医院 | 一种新型核酸测序*** |
| CN114540473B (zh) * | 2021-08-27 | 2024-03-01 | 四川大学华西第二医院 | 一种新型核酸测序*** |
| WO2023082305A1 (zh) * | 2021-11-09 | 2023-05-19 | 纳昂达(南京)生物科技有限公司 | 兼容双测序平台的建库元件、试剂盒及建库方法 |
| EP4202058A4 (en) * | 2021-11-09 | 2024-05-01 | Nanodigmbio Nanjing Biotechnology Co Ltd | BANK BUILDING ELEMENT COMPATIBLE WITH DOUBLE SEQUENCING PLATFORMS, BANK BUILDING KIT AND METHOD |
| WO2024119413A1 (zh) * | 2022-12-07 | 2024-06-13 | 深圳华大智造科技股份有限公司 | 基于一步法的双核酸纳米球制备方法 |
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