CN110066199A - A kind of high-quality Radix-paeoniae-rubra formula fertilizer and preparation method thereof and application method - Google Patents
A kind of high-quality Radix-paeoniae-rubra formula fertilizer and preparation method thereof and application method Download PDFInfo
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- CN110066199A CN110066199A CN201910357719.3A CN201910357719A CN110066199A CN 110066199 A CN110066199 A CN 110066199A CN 201910357719 A CN201910357719 A CN 201910357719A CN 110066199 A CN110066199 A CN 110066199A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B3/00—Fertilisers based essentially on di-calcium phosphate
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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Abstract
The invention belongs to fertilizer production and applied technical fields, and in particular to a kind of high-quality Radix-paeoniae-rubra formula fertilizer and preparation method thereof and application method.A kind of high-quality Radix-paeoniae-rubra formula fertilizer of the invention, including organic substance, nitrogen nutrition, phosphorus nourishing, potassium nutrition, active bio organic acid and environmentally friendly medicament, wherein organic matter content is 95%-98% by mass percentage, nitrogen nutrition, phosphorus nourishing, potassium nutrition content be 1.1%-1.5%, active bio organic acid content be 0.3%-0.4%.The invention proposes a kind of high-quality Radix-paeoniae-rubra formula fertilizer and preparation method thereof and application methods, active bio organic acid can significantly improve the rhizosphere soil environment of radix paeoniae rubra growth in the present invention, exogenous signals molecule contained therein has facilitation to Chinese herbaceous peony glycoside secondary metabolites synthesis in radix paeoniae rubra, facilitate the generation and accumulation of composition and effectiveness Paeoniflorin, Paeoniflorin average content improves 8.9% compared with the control.
Description
Technical field
The invention belongs to fertilizer production and applied technical fields, and in particular to a kind of high-quality Radix-paeoniae-rubra formula fertilizer and its preparation side
Method and application method.
Background technique
Chinese herbal medicine radix paeoniae rubra is the root of ranunculaceae plant Chinese herbaceous peony (Paeonia lactifolra Pall.) wild stocks, for me
State's tradition bulk medicinal materials.Alias wood radix paeoniae rubra, RADIX PAEONIAE RUBRA, red radix paeoniae rubra, careless radix paeoniae rubra, herbaceos perennial have clearing heat and cooling blood, dissipate
The effect of stasis of blood relieves pain.
The data retrieved according to inventor, current technical solution related to the present invention have:
Disclosing raw material in a kind of invention " preparation method of radix paeoniae rubra Chinese herbal medicine nutrient fertilizer " of publication No. CN101613243 A is
By dry human excrement, dry rabbit excrement, dried poultrymanure, dry duck excrement, rape cake, cottonseed cake, corn stalk, bran powder, soya bean stem meal, compound fertilizer, urea,
Calcareous fertilisers, zinc fertilizer, potash fertilizer, magnesium sulfate, DDVP, paclobutrazol powder, powdered glucose, epoxy resin composition.Powder is mixed in proportion
It is broken to be formed with human excrement water by fermentation.
Openly it is related to Chinese herbal medicine plantation fertilizer in a kind of invention " radix paeoniae rubra Chinese herbal medicine nutrient fertilizer " of publication No. CN101921153 A
Expect technical field, it is by dry cow dung, dry pig manure, dry goose excrement, beancake powder, corn flour, sorghum flour, wheat flour, green grass powder, wheat
Tangerine powder, potassium dihydrogen phosphate, coarse whiting, potassium sulfate, ammonium nitrate fertilizer, phosphate fertilizer, ammonium bicarbonate, metrifonate, carbendazim, powdered glucose, phenolic resin
Composition.Mixing human excrement water by fermentation forms in proportion.
Openly it is related to Chinese herbal medicine plantation fertilizer in a kind of invention " radix paeoniae rubra Chinese herbal medicine nutrient fertilizer " of publication No. CN102219594 A
Expect technical field, it is by dry sheep manure, dried poultrymanure, dry rabbit excrement, pumpkin powder, balsam pear powder, sorghum flour, pine needle meal, wood ash powder, beans tangerine
Powder, Cortex Eucommiae, potassium sulfate, plantago powder, radix aucklandiae, dried orange peel, insecticide, goes bacterium spirit, Luffa powder, phenolic resin group at potassium dihydrogen phosphate
At.Mixing human excrement water by fermentation forms in proportion.Goal of the invention is abundant for various nutrient contents, the speed of growth of radix paeoniae rubra after fertilising
Fastly, blade is plump, it is close to bloom, and as a result greatly, root is flourishing, and disease resistance is good, preventing disease and pest performance is strong.
It is public in a kind of invention " radix paeoniae rubra plantation nursery nutrient matrix and preparation method thereof " of publication No. CN106631420 A
Open and be related to Chinese herbal medicine field of planting, in invention matrix each raw material component in parts by weight, dry rabbit excrement is a, maize cob meal is a,
Peanut stem leaf powder is a, okara powder is a, Rape Straw is a, zinc fertilizer is a, potassium sulfate is a, auxin is a, epoxy resin
A, Chinese medicine slag portion includes a certain proportion of radix paeoniae rubra dregs of a decoction, the rhizoma cyperi dregs of a decoction, the Cortex Eucommiae dregs of a decoction and the dried orange peel dregs of a decoction.Preparation process packet
Include stock, fermentation, mixing, granulation and drying.
It is public in the invention " a kind of the phosphoric acid for preparing by the production method of by-product radix paeoniae rubra special fertilizer " of publication No. CN104229765 A
It opens and is related to chemical technology field, a kind of phosphoric acid for preparing is by the production method of by-product radix paeoniae rubra special fertilizer, which is characterized in that use phosphoric acid
It is mixed with urea according to molar ratio, is stirred to react acquisition intermediate slip at a certain temperature, then be passed through into intermediate slip
Ammonia by temperature control, is stirred to react, regulates and controls pH and obtain ammonium phosphate slip, then ammonium phosphate slip is placed in circulation temperature lowering crystallizer
Middle progress circulation temperature lowering crystallization treatment, is then filtered into ammonium phosphate product, and potassium composition and middle microelement are added into filtrate
Object is closed, and adjusts the molar ratio of n-p-k element ingredient in solution, drying can be prepared by the dedicated compound state composite fertilizer of radix paeoniae rubra.
The above-mentioned prior art is all fertilizer or Matrix formulation procedure about Chinese herbal medicine radix paeoniae rubra, is mainly characterized by various
Nutrient content is abundant, promotes radix paeoniae rubra fast-growth, root is flourishing, and diseases and insect pests resistance is strong, but excrement used and agrochemical are still
There are hormonal substances and heavy metal substance pollution risk.The present invention and foregoing invention difference are mainly manifested in improve Chinese herbal medicine
Rhizospheric environment and the active bio organic acid substance provided can facilitate radix paeoniae rubra Paeoniflorin to synthesize and accumulation, improve medicinal material product
Matter and yield cultivate Chinese herbal medicine radix paeoniae rubra for large-scale artificial and provide technical support.
Summary of the invention
The object of the present invention is to provide one kind not only can solve nutrient Regulation problem in artificial cultivation Chinese medicine, but also is able to achieve excellent
The high-quality Radix-paeoniae-rubra formula fertilizer of matter Chinese medicine large-scale production.
The object of the invention is also to provide a kind of preparation methods of high-quality Radix-paeoniae-rubra formula fertilizer.
The object of the invention is also to provide a kind of application methods of high-quality Radix-paeoniae-rubra formula fertilizer.
The object of the present invention is achieved like this:
A kind of high-quality Radix-paeoniae-rubra formula fertilizer, including organic substance, nitrogen nutrition, phosphorus nourishing, potassium nutrition, active bio have
Machine acid and environmentally friendly medicament, wherein organic matter content is 95%-98%, nitrogen nutrition, phosphorus nourishing, potassium by mass percentage
The content of nutrition is 1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio organic acid content are 0.3%-
0.4%, environmentally friendly chemical content is 0.1%-0.2%.
The organic substance includes at least one of lignite, turf.
In the nitrogen nutrition, one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate are included at least;The phosphorus element
In nutrition, one of calcium superphosphate, calcium monohydrogen phosphate are included at least;In the potassium nutrition, potassium sulfate and nitre are included at least
One of sour potassium.
The active bio organic acid includes at least one of salicylic acid, citric acid, lactic acid, formic acid.
The environmentally friendly medicament includes at least 40% pulvis pyrimethanil, 50% pulvis cyprodinil, 80% pulvis maneb
Two kinds in zinc.
A kind of preparation method of high-quality Radix-paeoniae-rubra formula fertilizer, includes the following steps:
(1) organic materials drying and crushing is crossed into 20 meshes, with nitrogen nutrition, phosphorus nourishing, potassium nutrition mass percent
Uniform compound;
(2) by the mixed material of step (1) and environmentally friendly medicament, abundant compound, shady place placement 24 are small by mass percentage
When;
(3) mixed material of step (2) is sufficiently mixed by mass percentage with active bio organic acid special up to radix paeoniae rubra
Use formula fertilizer;
Wherein organic matter content is 95%-98%, nitrogen nutrition, phosphorus nourishing, potassium nutrition by mass percentage
Content is 1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio organic acid content are 0.3%-0.4%,
Environmentally friendly chemical content is 0.1%-0.2%;The organic substance includes at least one of lignite, turf;The nitrogen
In nutrition, one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate are included at least;In the phosphorus nourishing, phosphorus was included at least
One of sour calcium, calcium monohydrogen phosphate;In the potassium nutrition, one of potassium sulfate and potassium nitrate are included at least;Described
Active bio organic acid includes at least one of salicylic acid, citric acid, lactic acid, formic acid;The environmentally friendly medicament includes at least
40% pulvis pyrimethanil, 50% pulvis cyprodinil, two kinds in 80% pulvis Mancozeb.
A kind of application method of high-quality Radix-paeoniae-rubra formula fertilizer, machinery, which is raised, before seedling replanting applies high-quality Radix-paeoniae-rubra formula fertilizer 1500-
3000 kilograms/667m2, rotary tillage 20-30cm, or high-quality 200-250 kilograms of Radix-paeoniae-rubra formula fertilizer/667m is applied in spring seedling-slowing stage cave2;
By mass percentage wherein organic matter content be 95%-98%, nitrogen nutrition, phosphorus nourishing, potassium nutrition content be
1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio organic acid content are 0.3%-0.4%, environmentally friendly medicine
Agent content is 0.1%-0.2%;The organic substance includes at least one of lignite, turf;In the nitrogen nutrition,
Including at least one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate;In the phosphorus nourishing, calcium superphosphate, phosphorus are included at least
One of sour hydrogen calcium;In the potassium nutrition, one of potassium sulfate and potassium nitrate are included at least;The active bio
Organic acid includes at least one of salicylic acid, citric acid, lactic acid, formic acid;The environmentally friendly medicament includes at least 40% pulvis
Pyrimethanil, 50% pulvis cyprodinil, two kinds in 80% pulvis Mancozeb.
The beneficial effects of the present invention are:
The invention proposes a kind of high-quality Radix-paeoniae-rubra formula fertilizer and preparation method thereof and application methods, and activity is raw in the present invention
Object organic acid can significantly improve the rhizosphere soil environment of radix paeoniae rubra growth, and exogenous signals molecule contained therein is to Paeoniflorin in radix paeoniae rubra
The synthesis of class secondary metabolites has facilitation, facilitates the generation and accumulation of composition and effectiveness Paeoniflorin, compared with the control Chinese herbaceous peony
Medicine glycosides average content improves 8.9%;Organic substance raw material natural green not only provides sufficient organic matter for radix paeoniae rubra, also has
The effect of radix paeoniae rubra paeoniflorin content is improved, provides nutrition assurance to produce high-quality genunie medicinal materials.Radix paeoniae rubra formula fertilizer special nutrition is taken
With reasonable, based on organic matter, cooperate n p k nutrition, up to 7.8% or more, selected medicament meets in country average rate of growth
Medicinal material medication register.Active bio organic acid can significantly improve the rhizosphere soil environment of radix paeoniae rubra growth, external source letter contained therein
Number molecule has facilitation to Chinese herbaceous peony glycoside secondary metabolites synthesis in radix paeoniae rubra, facilitates the generation of composition and effectiveness Paeoniflorin
And accumulation, radix paeoniae rubra Chinese herbal medicine quality is improved, radix paeoniae rubra gray mold, leaf spot and the generation of insect pest can be effectively prevented.
Detailed description of the invention
Fig. 1 is the variation diagram of Paeoniflorin after Radix-paeoniae-rubra formula fertilizer described in embodiment is applied 1 year.
Fig. 2 is the best relation map of adjusting control agent of the present invention application.
Specific embodiment
The present invention is described further with reference to the accompanying drawing.
Radix paeoniae rubra is perennial root plant, sprouts annual April and is unearthed, mid-May buddings, and blooms by the end of May to early June, opens
Take time to compare concentration, 10 days or so.6, root in July expands most fast, and early September aerial part starts withered and enters dormant period.9
The last ten-days period to the November moon is the root of hair phase, is most to contain with root of hair in October.Later with mercury dropped and root of hair is slow.The seed of Chinese herbaceous peony has
The habit of suspend mode, low-temperature treatment, gibberellin processing have the effect of breaking dormancy stratification.Paeonia lactiflora seed is preferably with adopting with broadcasting, or uses
Wet sand lamination cannot be dried in shady place, dried and be just not easy to germinate.Mid or late September sowing, takes root for the year after broadcasting.Chinese herbaceous peony is suitable
Mild climate, happiness is sunny, and then undergrowth, yield is not high greatly in the shade ground or shade rate.Cold-resistant, winter ridging can be got over safely
Winter.Before general late October frozen ground, branches and leaves is cut off at place from the ground, and earth up in rhizosphere, can protect and pass the winter.Radix paeoniae rubra also resistance to height
Temperature can be got over the summer under 42 DEG C of high temperature.It is drought-resistant, be afraid of humidity, be usually not required to fill general, be afraid of ponding.It is loose, fertile to soil requirement,
Soil layer is deeper, and draining is good, and preferably sand loam, folder husky of common dye soil or trip deposition sandy loam, salt-soda soil should not be planted.Avoid
Continuous cropping, can be with aster, safflower, chrysanthemum, legume crop rotation.It could be planted every 3-5.The present invention provides a kind of high-quality radix paeoniae rubra and matches
Square fertilizer and preparation method thereof and application method.The present invention includes:
(1) nutrition-allocated proportion: by lignite 2965kg dried and crushed, 20 mesh are crushed to, urea, calcium superphosphate, potassium sulfate are pressed
N:P2O5:K2O=1.3:1:1.52 ratio mixes 33kg, and lignite raw material and N P and K mixture uniform stirring is spare.
(2) after solid powder 0.6kg cyprodinil and Mancozeb being mixed by 1:1 with the abundant compound of (1) mixed material,
It places 24 hours, uniform with solid salicylic acid 1.4kg compound, places 24 hours up to radix paeoniae rubra formula fertilizer special.
(3) application method, radix paeoniae rubra formula fertilizer special machinery before radix paeoniae rubra transplantation of seedlings in 2 years, which is raised, applies 1500-3000 public affairs
Jin/667m2。
It is proposed that the embodiment of the present invention is as follows with reference to the accompanying drawing:
Embodiment 1
The present invention is intended to provide a kind of high-quality Radix-paeoniae-rubra formula fertilizer, including organic substance, nitrogen nutrition, phosphorus nourishing, potassium
Nutrition, active bio organic acid and environmentally friendly medicament, wherein organic matter content is 95%-98%, nitrogen battalion by mass percentage
Feeding, phosphorus nourishing, potassium nutrition content is 1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio have
Machine acid content is 0.3%-0.4%, and environmentally friendly chemical content is 0.1%-0.2%.The organic substance includes at least lignite, grass
One of charcoal.In the nitrogen nutrition, one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate are included at least;The phosphorus
In plain nutrition, one of calcium superphosphate, calcium monohydrogen phosphate are included at least;In the potassium nutrition, include at least potassium sulfate and
One of potassium nitrate.The active bio organic acid includes at least one of salicylic acid, citric acid, lactic acid, formic acid.Institute
The environmentally friendly medicament stated is including at least two kinds in 40% pulvis pyrimethanil, 50% pulvis cyprodinil, 80% pulvis Mancozeb.
Embodiment 2
It is also brown added with humification after the organic substance according to a kind of high-quality Radix-paeoniae-rubra formula fertilizer described in embodiment 1
The procedure of processing of coal, humification lignite includes:
(1) it roughing lignite: takes the static backlight ventilation that is placed on of lignite to dry, 60 meshes was smashed and ground, in temperature
It is dried in the drying box that variation range is 60-80 DEG C, the lignite after drying is placed in the container for storing of glass material;
(2) nitrify roughing lignite: take step (1) by rough machined lignite, additions mass fraction is 21%-24%
Nitric acid dousing 7-9 hours after, lignite is eluted to neutrality, is dried in 90 DEG C of drying box;
(3) demineralization lignite: the lignite of step (3) is mixed with the hydrochloric acid solution of 6mol/L 35:6 in mass ratio, uses glass
Glass vessel stir evenly, and are filtered after shaking 1 day on earthquake machine, are washed lignite to neutrality using distilled water;By lignite
It places and is dried under the conditions of 70 DEG C in a vacuum drying oven, 10 times 55% of hydrofluoric acid is added into the lignite that HCl treatment is crossed
Solution carries out concussion one day using oscillator, is filtered again, the lignite for again crossing HCl treatment is washed with distilled water to
Neutrality, and dried in 90 DEG C of drying boxes;
(4) activate lignite: lignite and the 50% phosphoric acid solution 6:1 in mass ratio containing 8% sulfuric acid for removing mineralising are mixed
It closes, after mixing evenly with glassware, is filtered after being impregnated 15 hours under the conditions of 90 DEG C, filtered raw material is put into perseverance
In warm furnace, it is passed through nitrogen, after so that lignite is reached 390 DEG C with the heating of 20 DEG C/min speed, is dried 1 hour, every 5 points in drying course
Clock leads to a nitrogen, and the temperature of lignite is made to be down to room temperature under field conditions (factors), is then washed to using 85-90 DEG C of distillation
Property, and dried in 90 DEG C of drying boxes;
(5) lignite humification: the sodium hydroxide solution of the lignite and mass fraction 30% that take step (4) to dry is in mass ratio
6:1 mixing, is stirred evenly with glassware, and room temperature is static to place two days, until mixed liquor layering, is taken lower sediment, is added distillation
Water stirs evenly, and room temperature is static to place two days, takes lower sediment, then plus distilled water repeated flushing, until the mixing of static placement
After liquid is not stratified, drying obtains finished product lignite in 90 DEG C of drying boxes.
The effect of humification lignite provided by the invention is adsorptional earth earth heavy metal ion, what heavy metal ion was adsorbed
Process can be divided into four steps: heavy metal ion is in the solution to lignite diffusion mobility;Heavy metal ion and lignite outer surface are living
The suction-operated in property site;Heavy metal ion diffusion mobility in lignite internal gutter;And table in heavy metal ion and lignite
Face active site suction-operated.
The relationship of the amount of the humification lignite and the amount of environmentally friendly chemical content are as follows:
RfAdsorbance when for adsorption equilibrium, RmMaximum adsorption capacity when for adsorption equilibrium, CfFor humification in adjusting control agent
The concentration of lignite;KLIt is constant related with activation energy of adsorption;KfThe parameter for characterizing adsorption capacity, n be adsorbent heterogeneity because
Son, R are gas constants, and T is absolute temperature, KTEquilibrium constant when being maximal absorptive capacity.
It can rationally be set before cultivating radix paeoniae rubra by the content of beary metal in rationally measurement plantation soil by above-mentioned relation
The lignite concentration in adjusting control agent is set, reaches save the cost and effectively adsorbs heavy metal simultaneously.It, can shadow since humification lignite amount is excessive
Cation content in soil is rung, causes soil acidity or alkalinity unbalance, while also will affect soil permeability ability, therefore the present invention may be used also
The problem of to avoid soil secondary pollution is excessively caused due to humification lignite amount.
Embodiment 3
The high-quality Radix-paeoniae-rubra formula fertilizer is also added with Chinese rhubarb extract, Chinese rhubarb extract the preparation method comprises the following steps: by rheum officinale
It crushes, the hydrochloric acid solution that mass concentration is 12wt%-14wt%, the bulking value of rheum officinale crushed material and the hydrochloric acid solution is added
Than being hydrolyzed 2 hours at 50-60 DEG C for 2:7-9g/mL, filtering, filter residue is washed to neutrality, dries, is 85%- with volume fraction
The envelope-bulk to weight ratio of 95% ethanol solution refluxing extraction, the ethanol solution and rheum officinale coarse powder is 10-15:1mL/g, is mentioned every time
2-3h is taken, repeats to extract 3-4 times, after filtering or centrifugation, liquid portion concentration and recovery ethyl alcohol to solid content is 10wt%-15wt%
Concentrate, concentrate is spray-dried at 160-180 DEG C, Chinese rhubarb extract.
Or rheum officinale 50g is weighed, and 12 times of amount distilled water are added every time, decocts and extracts 3 times, decocting extraction time is 1h/ times,
Merging filtrate after the Chinese rhubarb extract solution of 3 decoctions filters respectively;It is concentrated under reduced pressure under conditions of 80 DEG C, 0.07-0.09Mpa
To 100mL;At -40 DEG C to -44 DEG C, vacuum degree obtains Chinese rhubarb extract after being freeze-dried at 0.2-0.4mbar.
Wherein rheum officinale is at least one of root, stem, leaf of rheum officinale platymiscium.
Chinese rhubarb extract is that the drying root and rhizome of polygonum rheum palmatum, Rheum tanguticum Maxim or Rheum officinale extracts
Object.Sorrel and Rheum tanguticum Maxim medicinal material claim Beijing University yellow, and main product is in Qinghai, Gansu, Sichuan and other places.Rheum officinale medicinal material claims south
Rheum officinale, main product is in Sichuan, Hubei and other places.Rheum officinale bitter is cold, returns stomach, liver large intestine channel, and effective component is anthraquinone analog compound, including
Rhein, rheum emodin, Chrysophanol, aloe-emodin, Physcion etc. have scavenging activated oxygen, anti-oxidant, antibacterial, disease-resistant
Poison and other effects.
In Chinese rhubarb extract of the invention, general anthraquinone kind compound content is 40-50wt%.Dry powder made of extract
In, the mass ratio of combined anthraquinone and sequestered anthraquinone is 1:7-10.
Concentration of the Chinese rhubarb extract in adjusting control agent are as follows:
Qt=Ktt0.5+C;
KtFor diffusion rate constant in particle, C is constant related with soil horizon thickness, and t is adjusting control agent effective time.
Embodiment 4
High-quality Radix-paeoniae-rubra formula fertilizer of the invention is also added with fir-needle extract, preparation method are as follows:
Take fir-needle to clean and place in the cool dry after, be smashed into fine particle with pulverizer;By fir-needle powder mistake
It is packed into plastic packaging bag after 0.3 mesh, is saved under the conditions of 4 DEG C;
Take fir-needle powder, every 5g is carried out circumfluence distillation 2 hours or more under the conditions of 80 DEG C of 50mL distilled water;Filtering
Extracting solution collects filtrate and is settled to 50mL with pure water, and decompression filters and obtains fir-needle extract;Or take fir-needle
The every 5g of powder with 50mL mass fraction be 50% ethanol solution by 80 DEG C circumfluence distillation 2 hours or more, filter extracting solution,
It collects filtrate and is settled to 50mL with the ethanol solution that mass fraction is 50%, decompression filters and obtains fir-needle extract;Or
Person takes fir-needle powder, and every 5g is carried out ultrasonic wave extraction 30 minutes or more with 50mL distilled water, filters extracting solution, collects filtrate
And it is settled to 50mL with pure water, decompression filters and obtains fir-needle extract.
Fir belongs to the pine tree section plant of high latitude, High aititude, and before the centuries, locals has just recognized the medicine of fir
With and health value.Fir essence is to be extracted based on the extract of the fresh needle cell liquid of 100% fir using liquefied carbon dioxide
It takes and advanced micro-capsule technique for packing is made.After testing, it is had found in Abies sibirica essence a kind of extremely precious
Dilute ingredient --- polypentenol.Except polypentenol, fir essence also contains iron-maltol, aminoacetic acid, phosphatidyl silk ammonia
The ingredients such as acid, phenolic acid, Vitamin E acetate.Iron-maltol is extremely rare element, because of iron content-maltol, keeps fir smart
China is in peony.Maltol is a kind of natural natural, on the one hand, it blocks free radical, prevents it from destroying cell
With the integrality of fibrous body tissue.And on the other hand, it can promote plant that ferro element is preferably absorbed and digested.
The present invention extracts the extracts such as triterpenic acid and the polypentenol in separation fir-needle using fir-needle as raw material,
The medical value for developing these bioactive substances makes needle liposoluble substance obtain more reasonable utilization.Using solvent extraction
Method and chromatography, the selectivity and recovery rate of extraction greatly improve, and products obtained therefrom purity is also higher, and the recyclable repetition of solvent
It utilizes, environment is more friendly.The present invention makes full use of triterpenic acid and polypentenol in needle liposoluble substance etc., improves production
The total yield of product, reduces production cost, is a kind of extraction separation method with popularization and application foreground.It extracts and separates from fir
Needle triterpenic acid can influence the physiology course of radix paeoniae rubra growth and development using very small dosage, be mainly manifested in raising
The yield of radix paeoniae rubra has apparent promotion production-increasing function, and when being used in combination with other existing pesticides, effect of increasing production is clearly.
Embodiment 5
High-quality Radix-paeoniae-rubra formula fertilizer of the invention is also added with ginseng endophyte extract, preparation method are as follows:
It is carried out disinfection with mercury chloride, sodium hypochlorite and dehydrated alcohol to ginseng root system surface, using Tissue blot-ELISA inspection
Whether ginseng surface sterilization is thorough;Ginseng endophyte is separated with tissue isolation, with No. 1 culture medium of Gao Shi, internally life is put
Line bacterium is separated, is separated with PDA culture medium to endogenetic fungus, being separated with NA culture medium to endogenetic bacteria;
Configuration fermentation liquid culture medium, basal culture medium include that yeast extract quality is 3.5g, Na2HPO4Quality is 17g, MgSO4
Quality is 0.2g, soluble starch quality is 3.5g, Na2SO4Quality is 1.5g, peptone quality is 17g, H2O capacity is
1000mL, being put into high-pressure sterilizing pot in order, and start to sterilize under the conditions of 100 DEG C, 30 minutes.After subject to sterilization,
The bacterium colony 2-3 block for the endogeny rayungus, endogenetic fungus or endogenetic bacteria that diameter is 7-9mm size is taken in aseptic superclean bench
In access fermentation liquid culture medium, temperature is placed in cultivate 36 hours on 30 DEG C of shaking table after sealing culture medium, is 125 in revolving speed
Under the conditions of rev/min, shake culture 6 days, fermentation liquid is obtained.Then fermentation liquid is filtered with suction filtration machine, and will be obtained after filtering
To fermentation liquid and mycelium got up respectively with container collection.
Filtrate and mycelium are mixed after mycelium and fermentation liquid are extracted repeatedly respectively with ethyl acetate solvent.
The mixture carries out concentrated by rotary evaporation to the 50% of original volume with Rotary Evaporators, is collected into concentrate, and concentrate is drained and is flowed into
The silicagel column of silica gel has been installed, has been then that 1:50 ethyl acetate and petroleum ether solvent carry out gradient elution with quality proportioning, has been used
Thin layer chromatography is compared, and merges wherein identical component, obtains 3 components, and it is 15 that the first component, which is passed through quality proportioning:
Ethyl acetate, the second alcohol and water of 13:2 carries out gradient elution, recrystallization, obtains the first compound.Second component is matched by quality
Than the chloroform and methanol progress gradient elution, recrystallization for 25:1, second compound is obtained.Third component uses anhydrous second
Alcohol is eluted, is recrystallized, and obtains third compound.
Or extract fermentation liquid and mycelium repeatedly respectively with n-butanol solvent, filtrate and mycelium are combined.
Mixture after merging carries out concentrated by rotary evaporation with Rotary Evaporators and is collected into concentrate 20g, and concentrate is drained with glass bar and flowed into
The silicagel column of silica gel is installed, the ethyl acetate and petroleum ether solvent for being then 1:75 with quality proportioning carry out gradient elution, adopt
It is compared with TLC, merges wherein identical component, therefrom obtain 4 components, the 4th component has Precipitation, with acetic acid second
Ester dissolves the 4th component, prepares thin layer, and chloroform and methanol solution that quality proportioning is 7:1 are unfolded, two colour bands occurs, scrapes
Two colour bands, respectively obtain the 8th component and the 9th component, and the chloroform for being 17:5:2 with quality proportioning, first alcohol and water continue gradient
The the 8th and the 9th component is eluted, obtains fourth compound and the 5th compound respectively after repeated recrystallize;By fifth component TLC
It is compared, merges wherein identical component, then the chloroform and methanol solution for being 30:1 with quality proportioning continue gradient and wash
It is de-, it is crystallized and is recrystallized after elution, obtain the 6th compound;6th component is compared with TLC, is merged wherein identical
Component, then the chloroform and methanol solution that are 50:1 with quality proportioning continue gradient elution, carry out crystallizing and tying again after elution
Crystalline substance obtains the 7th compound;7th component first obtains the tenth component, again with methanol, ethyl acetate with ethyl acetate solvent elution
Gradient elution is carried out with the mixture of chloroform, it is crystallized and is recrystallized after elution, obtains the 8th compound.
By above-mentioned eight kinds of compounds proportionally mixed grinding at powder obtain ginseng endophyte extract.
Xanthinin, isotanshinone II, Panaxynol, 2,4,5- trimethyl-can be obtained in the endophyte separated in ginseng
1,3- benzenediol, 2,4- dihydroxy -3,5,6- methyl trimethylbenzene, mannitol, penicillic acid, ergosterol, peroxide ergot
Sterol, to compounds such as hydroxyethyl phenols, which can stimulate plant growth by different modes, can be with itself conjunction
At or promote growth hormone necessary to host plant synthesis plant and promote the growth of host's root system and each organ, assist host
Plant absorbs nutritious mineral element in the soil, can also be by enhancing host plant to severe growing environment and harmful cause of disease
Defence capability of body etc. grows plant stability, and the bioactivity having is also varied, as antiviral activity,
Enzyme inhibition activity, immunosuppressive activity, insecticidal activity, antimicrobial acivity, antioxidant activity, Anti-protozoal activity.
Embodiment 6
High-quality Radix-paeoniae-rubra formula fertilizer of the invention is also added with four-o'clock methanolic extract, and extracting method includes:
Four-o'clock crushed material is taken, with the methanol of 10 times of quality to the extraction of four-o'clock crushed material 48 hours or more, to above-mentioned molten
Liquid is filtered, and the residue being obtained by filtration continues to use same method and extracted 48 hours by methanol to four-o'clock crushed material,
And the extracting solution filtered twice merging is concentrated to the 1/2 of original volume, organic solvent is recycled, four-o'clock methanol is obtained and extracts
Object.
Four-o'clock (Mirabilis jalapa L.), is called four-o'clock flower, Radix primulae maximowiczii or powder Tofu pudding, belongs to Nyctaginaceae, purple
Jasminum is a kind of perennial ornamental flower, often makees annual cultivation, highly up to 1m.South America is originated in, there is ecologic adaptation
Extensively, the biological natures such as impoverishment tolerant, the speed of growth are fast, reproductive capacity is strong have cultivation in many areas in China.Contain amino in root
Acid, organic acid and much starch, flower can be used as medicine containing uranidin, root and leaves such as a variety of betaxanthins, have clearing heat and detoxicating, promoting blood circulation
The effect of menstruation regulating and nourishing.In recent years, the research of four-o'clock is paid attention to, in terms of medicine, plant protection, environmental protection
Show researching value.Inventor is the study found that four-o'clock has the potentiality for preparing botanical pesticide.
Discovery four-o'clock methanolic extract of the invention has an Antiphytoviral effect, and extract is to diamondback moth, cabbage butterfly, tiltedly
Autographa spp etc. has killing effect.
Embodiment 7
High-quality Radix-paeoniae-rubra formula fertilizer of the invention is also added with plant endogenesis fungus extract, production method are as follows:
The leaf for taking plant rinses the impurity on blade well with pure water, dries blade surface moisture in the cool;Into
The disinfection treatment of the row page: rinsing plant leaf 5min or more with 70% alcohol of concentration, with pure water rinse plant leaf 3 times with
On;Then 5min or more is rinsed with the liquor natrii hypochloritis of concentration 3%, then with pure water rinse plant leaf 3 times or more, in yin
Liang Chu dries blade surface moisture, and the blade cuts after above-mentioned surface sterilization are placed in PDA culture medium at fritter, at 30 DEG C
It is cultivated 8 days under isoperibol.
Mycelia is transferred in another PDA culture medium with the tweezers of disinfection after the small block edge of blade grows mycelia, using flat
Plate scribble method purify bacterium colony, it is to be purified completely after, take bacterial strain after purification, bacterial strain carried out liquid fermentation, filtering fermentating liquid,
It takes filtrate that ethyl acetate is added to be extracted, extracts 2 times.Combining extraction liquid evaporated under reduced pressure under the conditions of 40 DEG C, obtains coarse extract i.e.
Secondary metabolite.
Or in the conical flask for the 250ml capacity for taking mycelia to be transferred to the PDB culture solution equipped with 100ml, 30 DEG C of progress,
The constant-temperature shaking culture of 160rpm, incubation time 60 hours.Bacterium solution in conical flask is inoculated in the culture of the PDB equipped with 500ml
In the conical flask of the 1000ml capacity of liquid, 30 DEG C, the constant-temperature shaking culture of 160rpm are carried out, incubation time 240 hours.It is right respectively
Above two bacterium solution and mycelia are extracted with ethyl acetate, are extracted 5 times.Extract liquor is subjected to decompression steaming under the conditions of 40 DEG C
It is dry, obtain fermentation crude extract.Methanol dissolves bacterium solution and mycelia, and equivalent silica gel is added to stir, with petroleum ether wet method dress post, using petroleum
Ether: acetone is 5:0.7-1:9 and methylene chloride: the solution of methanol 12:1-0:1 carries out various concentration gradient elution and obtains extract
Component is compared using TLC, is merged wherein identical component, is obtained a thick component extract, is dissolved with chloroform and methanol
After stand, be precipitated clear crystals in solution, obtain plant endogenesis fungus extract after cleaning crystal surface impurity.
The plant endogenesis fungus extract includes penicillic acid, ergosterol, dihydro penicillic acid, thymidine, indoles -3-
Formic acid.
The plant includes short leaf purple sweater bark, Chinese littleleaf box leaf, lyme grass leaf, kamuning leaf, tomato leaves, fragrant-flowered garlic
Dish leaf, Chinese yam leaf, pomegranate leaf and granatum.
Endophyte of plant refers to those its all or part of history of life in the various tissues or cell interior degree of health plant
The fungi crossed or bacterium (including actinomyces) exist and do not cause apparent host infection symptom, but can pass through Histological method
Or it separates from the plant tissue of stringent surface sterilization or goes out the method for microbial DNA from inside plant tissues plant expansion to demonstrate,prove
Its bright endogeny.Its root for being widely distributed in plant, stem, leaf, flower, in fruit, harmless to host plant, host plant mentions for it
For the nutriment needed for growing, promote host plant synthesizing activity ingredient, and help host plant metabolic toxicities, resists
Extraneous attack and invade food.The two mutualism, antagonism balance.The type and quantity of endophyte of plant by host plant type and
External environment influences very big.Different plant strains are different, and kindred plant different location, different temperatures, varying environment strain are different,
It can be different same plant different time strain.As a kind of microorganism new resources, growth cycle is short, metabolism is easy to control
System, strain are easy to breeding and can realize that the advantage of industrialized production etc. makes people to its growing interest by large scale fermentation.
To of the invention research shows that: negative is presented in the concentration and tested person seed germination rate of plant endogenesis fungus extract
It closes.When endophyte of plant extract solution concentration is 2mg/ml, the bud ratio of barnyard grass, field bindweed and black nightshade seed is 0.Work as plant
When object endophyte extract solution concentration is 2.25mg/ml, the bud ratio of Amaranthus retroflexus, herba setariae viridis and Cowhells grass seed is 0.Cause
This, the present invention is more obvious for the herbicidal effect of radix paeoniae rubra.
Embodiment 8
The present invention increases cellulose-degrading bacteria, specific cultural method in organic substance are as follows:
10g cellulose-degrading bacteria is added in the enriched medium of sterilized 110ml, it is permanent at 220r/min, 35 DEG C
Temperature oscillation 240 days.Then the bacterium solution of 100ml is taken to be centrifuged 20min under conditions of 3000r/min, with pure book by 5ml supernatant
Gradient dilution is carried out, the dilution for drawing 0.1ml respectively carries out painting work on CMC plate, and each gradient is repeated 3 times.?
After cultivating 120 hours under 35 DEG C of constant temperature, picking single colonie further separates on CMC culture medium, purifies culture, the bacterium colony of purifying
Dibbling is repeated 3 times, cultivates 144 hours at 35 DEG C on CMC plate respectively, with the Congo red dye liquor of 2g/L, dyes 1 hour, filter
Fall dye liquor, add the NaCl solution of 1mol/L, washs 1 hour.
Whether there is or not apparent transparent circle and the transparent loop diameter of statistics and colony diameter are measured at detection bacterium colony.By transparent loop diameter
Bacteria suspension is prepared with the maximum bacterial strain of ratio of colony diameter, 3ml is pipetted respectively and is inoculated into filter paper culture medium, in 35 DEG C of perseverances
It is cultivated 240 hours in warm incubator, observes the variation of filter paper item, filter paper item is selected to be degraded degree highest bacterium bacterial strain inoculation
To LB culture medium, under the conditions of 240r/min, 35 DEG C after constant-temperature shaking culture 25 hours, DNA extraction and PCR amplification are carried out, so
Detection PCR product is carried out with the agarose gel electrophoresis of mass fraction 1% afterwards.Bacterial strain is sequenced through 6SrDNA, utilizes BLAST function
The gene order measured is carried out tetraploid rice by component, chooses the higher bacterial strain of similitude as cellulose-degrading bacteria.
In composting process, it be unable to do without the leading role of various microorganisms.Although it is slightly raw that straw compost itself contains one
Object, but since the labyrinth of lignocellulosic in stalk ingredient limits decomposition rate, only a small number of microorganisms play decomposition
Effect, therefore researcher is added in composting process in order to improve composting efficiency and promotees rotten microbial inoculum, to supplement micro- life in compost
Species increase micro organism quantity, and the enzyme that microorganism generates is richer, and Each performs its own functions and cooperation for each strain, in compost
In make the quick decomposition of organic matter, compost maturity is faster.
Embodiment 9
The high-quality Radix-paeoniae-rubra formula fertilizer is sprinkled into garlic root decomposition liquid, the system of decomposition liquid after using 3 days into soil
Make method are as follows:
It weighs 20g and shreds into the garlic root of 0.5cm length and be placed in 3L glassware, it is molten that 2L soil dilution is added
Liquid is put into 30 DEG C of constant incubators and is protected from light culture.Decomposition liquid is extracted after 25 days: being filtered by vacuum after filtering 2 times, uses acetic acid
Ethyl ester, which sufficiently extracts 40 DEG C of rotary evaporations after liquid separation and is concentrated into 15mL, obtains garlic root decomposition liquid, is placed in 5 DEG C of refrigerators and saves.
Soil enzyme is the active components in soil constitution, participates in numerous metabolic activities in soil, close with soil fertility
It is related.Garlic can be improved phosphatase activity in soil, promote phosphorus element turnover, circulation, the reason is that it can promote the micro- life of rhizosphere
Object diversity increases.Garlic stalk decomposition object can significantly improve soil pH and soil conductivity value, and straw decomposition object can be
Increase full nitrogen and organic carbon content in soil to a certain extent, improves invertase, urase and alkaline phosphatase activities in soil.
Embodiment 10
It should be pointed out that endophyte of plant of the present invention and four-o'clock methanolic extract (or ginseng endophyte extract)
The case where promoting mutually and restricting is presented with ratio variation in proportional amount.For example, endophyte of plant has the effect of weeding, so
And four-o'clock methanolic extract and ginseng endophyte extract have inhibiting effect to it, it is excessive due to being related to experimental data amount,
Here the experimental data mutually promoted and inhibited is not illustrated one by one.
The present invention is in application, after concentrating each component content to be adjusted regulation, surveys radix paeoniae rubra seedling growth rhythm,
It is fitted by data, we have obtained the best relation map of the long height of seedling degree (cm) of radix paeoniae rubra and the application of inventive formulation fertilizer:
Wherein t is the content of endophyte of plant, and 1.5%≤t≤2.2%, T are raw in four-o'clock methanolic extract or ginseng
The content of fungus extract, 0.8%≤T≤1.5%, G are formula fertilizer per kilogram per hectare amount of application, 700≤G≤770, by above formula
Parameter unified representation comprising endophyte of plant and four-o'clock methanolic extract (or ginseng endophyte extract) in bracket is
A is put as shown in Fig. 2, wherein curve is matched curve as actual samples point.
In conclusion: the invention belongs to fertilizer production and applied technical fields, and in particular to a kind of high-quality Radix-paeoniae-rubra formula fertilizer
And preparation method thereof and application method.Invention is the reasonably combined nutrient based on organic matter, by adding bioactivity
Acid is combined formula fertilizer special in conjunction with environmentally friendly medicament, has not only been suitble to radix paeoniae rubra to need fertile Rules of Nutrition and physiological property, but also meet medium-height grass
The high standard demand of medicine production is especially improving rhizosphere soil environment, is improving significant effect in terms of radix paeoniae rubra Paeoniflorin, is medium-height grass
Medicine artificial cultivation large-scale planting and high-quality production of crude drugs provide technical support, and economic benefit, ecological benefits are obvious.The formula fertilizer
It is a kind of biological organic compound preparation, ingredient includes that organic substance, nitrogen, phosphorus, potassium, active bio organic acid and environmentally friendly medicament are pressed
Mass percent wherein organic materials content 95%-98%, n p k nutrition content 1.1%-1.5%, N:P2O5:K2O=1.3:
1:1.52, active bio organic acid content 0.3%-0.4%, environmentally friendly chemical content 0.1%-0.2%.The organic substance is extremely
It less include one of lignite, turf;In the nitrogen nutrition, including at least in urea, ammonium sulfate, ammonium acetate and ammonium nitrate
One kind in the phosphorus nourishing, including at least one of calcium superphosphate, calcium monohydrogen phosphate, and the potassium nutrition at least wraps
Include one of potassium sulfate and potassium nitrate;The active bio organic acid includes at least salicylic acid, citric acid, lactic acid, formic acid
One of;The environmentally friendly medicament includes at least 40% pulvis pyrimethanil, 50% pulvis cyprodinil, 80% pulvis maneb
Two kinds in zinc.Organic substance drying and crushing is first crossed 20 meshes by preparation method, uniformly multiple by mass percentage with nitrogen, phosphorus, potassium
It is mixed.Again by above-mentioned compound object and environmentally friendly medicament abundant compound by mass percentage, after shady place is placed 24 hours, then it is raw with activity
Object organic acid is sufficiently mixed by mass percentage up to radix paeoniae rubra formula fertilizer special.Application method, the radix paeoniae rubra formula fertilizer special exist
Machinery, which is raised, before seedling replanting applies 1500-3000 kilograms/667m2, rotary tillage 20-30cm, or 200-250 public affairs are applied in spring seedling-slowing stage cave
Jin/667m2。
Here it must be noted that other unaccounted technologies that the present invention provides are because be all the known skill of this field
Art, title or function according to the present invention, those skilled in the art can find the document of related record, therefore not do
It further illustrates.The technical means disclosed in the embodiments of the present invention is not limited only to technological means disclosed in above embodiment, also
Including technical solution consisting of any combination of the above technical features.
Claims (7)
1. a kind of high-quality Radix-paeoniae-rubra formula fertilizer, including organic substance, nitrogen nutrition, phosphorus nourishing, potassium nutrition, active bio are organic
Acid and environmentally friendly medicament, it is characterised in that: wherein organic matter content is 95%-98%, nitrogen nutrition, phosphorus element by mass percentage
Nutrition, potassium nutrition content be 1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio organic acid content
For 0.3%-0.4%, environmentally friendly chemical content is 0.1%-0.2%.
2. a kind of high-quality Radix-paeoniae-rubra formula fertilizer according to claim 1, it is characterised in that: the organic substance includes at least
One of lignite, turf.
3. a kind of high-quality Radix-paeoniae-rubra formula fertilizer according to claim 1, it is characterised in that: in the nitrogen nutrition, at least
Including one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate;In the phosphorus nourishing, calcium superphosphate, phosphoric acid hydrogen are included at least
One of calcium;In the potassium nutrition, one of potassium sulfate and potassium nitrate are included at least.
4. a kind of high-quality Radix-paeoniae-rubra formula fertilizer according to claim 1, it is characterised in that: the active bio organic acid is extremely
It less include one of salicylic acid, citric acid, lactic acid, formic acid.
5. a kind of high-quality Radix-paeoniae-rubra formula fertilizer according to claim 1, it is characterised in that: the environmentally friendly medicament includes at least
40% pulvis pyrimethanil, 50% pulvis cyprodinil, two kinds in 80% pulvis Mancozeb.
6. a kind of preparation method of high-quality Radix-paeoniae-rubra formula fertilizer, characterized by the following steps:
(1) organic materials drying and crushing is crossed into 20 meshes, it is uniform with nitrogen nutrition, phosphorus nourishing, potassium nutrition mass percent
Compound;
(2) by the mixed material of step (1) and environmentally friendly medicament, abundant compound, shady place are placed 24 hours by mass percentage;
(3) mixed material of step (2) is sufficiently mixed by mass percentage with active bio organic acid and is matched up to radix paeoniae rubra is dedicated
Side's fertilizer;
By mass percentage wherein organic matter content be 95%-98%, nitrogen nutrition, phosphorus nourishing, potassium nutrition content
For 1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio organic acid content are 0.3%-0.4%, environmental protection
Chemical content is 0.1%-0.2%;The organic substance includes at least one of lignite, turf;The nitrogen nutrition
In, include at least one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate;In the phosphorus nourishing, peroxophosphoric acid is included at least
One of calcium, calcium monohydrogen phosphate;In the potassium nutrition, one of potassium sulfate and potassium nitrate are included at least;The work
Property biogenic organic acid include at least one of salicylic acid, citric acid, lactic acid, formic acid;The environmentally friendly medicament includes at least
40% pulvis pyrimethanil, 50% pulvis cyprodinil, two kinds in 80% pulvis Mancozeb.
7. a kind of application method of high-quality Radix-paeoniae-rubra formula fertilizer, it is characterised in that: machinery, which is raised, before seedling replanting applies high-quality Radix-paeoniae-rubra formula
Fertile 1500-3000 kilograms/667m2, rotary tillage 20-30cm, or high-quality Radix-paeoniae-rubra formula fertilizer 200-250 public affairs are applied in spring seedling-slowing stage cave
Jin/667m2;Wherein organic matter content is 95%-98%, nitrogen nutrition, phosphorus nourishing, potassium nutrition by mass percentage
Content is 1.1%-1.5%, wherein N:P2O5:K2O=1.3:1:1.52, active bio organic acid content are 0.3%-0.4%,
Environmentally friendly chemical content is 0.1%-0.2%;The organic substance includes at least one of lignite, turf;The nitrogen
In nutrition, one of urea, ammonium sulfate, ammonium acetate and ammonium nitrate are included at least;In the phosphorus nourishing, phosphorus was included at least
One of sour calcium, calcium monohydrogen phosphate;In the potassium nutrition, one of potassium sulfate and potassium nitrate are included at least;Described
Active bio organic acid includes at least one of salicylic acid, citric acid, lactic acid, formic acid;The environmentally friendly medicament includes at least
40% pulvis pyrimethanil, 50% pulvis cyprodinil, two kinds in 80% pulvis Mancozeb.
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