CN110050708A - A kind of weanling pig growth fermenting equalizing bed padding and preparation method thereof - Google Patents
A kind of weanling pig growth fermenting equalizing bed padding and preparation method thereof Download PDFInfo
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- CN110050708A CN110050708A CN201910496131.6A CN201910496131A CN110050708A CN 110050708 A CN110050708 A CN 110050708A CN 201910496131 A CN201910496131 A CN 201910496131A CN 110050708 A CN110050708 A CN 110050708A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
A kind of weanling pig growth fermenting equalizing bed padding, using fermenting agent and fermentation substrate as raw material, prepare by heap fermentation, fermentation substrate includes following parts by weight material: 10-20 parts of bagasse, eats mushroom bran 40-60 parts, 10-20 parts of cotton seed hulls at 10-20 parts of vinegar grain；The parts by weight of fermenting agent are 1-5 parts；Living bacteria count >=10 in fermenting agent8Cfu/g, efficient bacterium group therein are combined into bacillus+saccharomycete+lactic acid bacteria, and meet following living bacteria count ratio, bacillus: saccharomycete: lactic acid bacteria=1-5:0.5-1.5:1-3.Prepared by the method fermenting bed padding feeds weanling pig, can be effectively reduced the stress reaction of weanling pig wean, promotes its feed, improve its growth performance and immunocompetence.
Description
Technical field
The invention belongs to technical field of animal husbandry, and in particular, to a kind of weanling pig growth fermenting equalizing bed
Padding and preparation method thereof.
Background technique
Fermentation bed to raise pig technology is that plant layoutization pig raising develops to certain phase and a bright spot being formed, is that livestock and poultry are supported
Grow a kind of new model of industry sustainable development.Fermentation bed to raise pig technology is not only that pig provides vegetative activity field using inertia padding
Institute also provides growing multiplication carrier for microorganism, thus forms the efficient breeding farm of a probiotics, Pig dung and urine warp and padding
After being sufficiently mixed, is degraded rapidly, converts as nutrition by probiotics, while also reducing the generation of pernicious gas, can significantly drop
Foul smell and peculiar smell are expected to realize pig raising without discharge, pollution-free environment without foul smell, thorough solution scale pig farm in low farm
Pollution problem.On the other hand, contain a large amount of beneficial microbes in fermentation bed column home perhaps, and become dominant microflora, can effectively hinder
Gear and the growth and breeding for inhibiting pathogen, provide favorable environment for pig healthy growth.In addition, being rich in the padding quilt of probiotics
After pig feeding, probiotics plays a role in enteron aisle, inhibits harmful bacteria, enhances immunity of organisms, also plays improvement pig
The effect of health status.
Currently, promote the research in growing and fattening stage growing development of live pig relatively broad fermentation bed to raise pig technology,
However, being still relatively short of for the research of the impact effect of the upgrowth situation of weanling pig fermentation bed to raise pig technology.
Summary of the invention
The object of the present invention is to provide a kind of weanling pig growth fermenting equalizing bed paddings and preparation method thereof, to solve
At least one of above-mentioned technical problem.
According to an aspect of the present invention, a kind of weanling pig growth fermenting equalizing bed padding is provided: using zymophyte
Agent and fermentation substrate are prepared as raw material by heap fermentation, and fermentation substrate includes following parts by weight material: bagasse 10-20
Part, 10-20 parts of vinegar grain, edible 40-60 parts of mushroom bran, 10-20 parts of cotton seed hulls；The parts by weight of fermenting agent are 1-5 part；Zymophyte
Living bacteria count >=10 in agent8Cfu/g, efficient bacterium group therein is combined into bacillus+saccharomycete+lactic acid bacteria, and meets
Following living bacteria count ratio, bacillus: saccharomycete: lactic acid bacteria=1-5:0.5-1.5:1-3.
Preferably, the living bacteria count in fermenting agent meets: bacillus: saccharomycete: lactic acid bacteria=3:1:2.
Preferably, bacillus is selected from bacillus subtilis, bacillus licheniformis, bacillus coagulans, waxy gemma bar
One of bacterium, bacillus pumilus, clostridium butyricum are a variety of.
Preferably, bacillus is made of bacillus cereus, bacillus licheniformis and clostridium butyricum, and satisfaction has as follows
Imitate viable count ratio: bacillus cereus: bacillus licheniformis: clostridium butyricum=1:1:2.
Preferably, edible mushroom bran includes needle mushroom mushroom bran peace mushroom chaff.
Preferably, according to parts by weight, mushroom bran is eaten to be made of 30 parts of needle mushroom mushroom brans and 25 parts of oyster mushroom mushroom brans.
Preferably, fermentation substrate includes 15 parts of vinegar grain, eats 50 parts of mushroom bran, 3 parts of fermenting agent, 0-10 part of calcium monohydrogen phosphate,
Raw material further includes 3-5 parts of brown sugar.
According to another aspect of the present invention, a kind of preparation of above-mentioned weanling pig growth fermenting equalizing bed padding is provided
Method, which comprises the following steps: fermenting agent is added to activate in brown sugar water and be trained by step (1) with the amount of 5wt%
20-30h are supported, activation zymocyte liquid is obtained；Step (2), material needed for weighing fermentation substrate stir and evenly mix, and obtain fermentation bottom
Object；Step (3) will activate zymocyte liquid inoculation fermentation substrate；Step (4) adds water to adjust fermentation substrate, makes containing for fermentation substrate
Water is 55-65%；Step (5), heap fermentation 6-8 days.
Preferably, in step (2), the parts by weight of control bagasse, cotton seed hulls and calcium monohydrogen phosphate make fermentation substrate
Carbon-nitrogen ratio C/N=40-60:1, carbon-phosphorus ratio C/P=80-140:1.
Preferably, in step (4), after the water content adjusting for completing fermentation substrate, the pH value of fermentation substrate is adjusted, is made
Its pH=6-8.
The present invention selects vinegar grain, needle mushroom mushroom bran peace mushroom chaff to cooperate other auxiliary materials as preparing fermenting bed padding:
(1) promote the growth and development of weanling pig
The faint scent mushroom taste that vinegar fragrance that vinegar grain has, edible mushroom bran have can promote the appetite of pig, improve wean
The daily ingestion amount of piglet；The material system compound nutritional principle of complementarity being made of above-mentioned material, pig, can by arch food padding
The abundant and balanced nutriment of supplement；Edible mushroom bran stimulation weanling pig secretes various digestive ferments, improves weanling pig pair
Feed digests and absorbs rate.
(2) immunocompetence of weanling pig is improved
Vinegar grain can play the role of inhibiting harmful pathogen, reduce the etiology in pig house；Contain one in edible mushroom bran
Quantitative profitable strain, these probiotics with weanling pig encircle food padding enter in weanling pig body and in its enteron aisle it is default
It plants, certain inhibiting effect can be played to the pathogen in its enteron aisle, thus improve the immunocompetence of weanling pig.
In addition, the present invention is inoculated with specific microorganism into fermenting bed padding raw material, specific strain combination is in fermentation body
It is synergistic in system, higher activity is kept, and the material in fermentation substrate is more rapidly effectively converted into weanling pig energy
The nutriment being enough absorbed and utilized.Pig enters the abundant microorganism in padding in vivo, is effectively adjusted by arch food padding
The distribution of its intestinal microflora, plays a driving role to proliferation of probiotics, has been proliferated inhibiting effect to harmful bacteria, has further mentioned
The high immunocompetence of pig.In addition, the excrement of weanling pig discharge is effectively decomposed by the abundant microorganism in padding, reduce
Since pig house hoards threat of causing a disease caused by waste.
Detailed description of the invention
Fig. 1 is the test pig enteron aisle E. coli counts statistical chart of embodiment 1；
Fig. 2 is the test pig Salmonella enteritidis counting statistics figure of embodiment 1；
Fig. 3 is the test Lactobacillus sp.from piglet intestine counting statistics figure of embodiment 1；
Fig. 4 is the test pig intestinal bifidobacteria counting statistics figure of embodiment 1；
Fig. 5 is the test pig enteron aisle total protein hydrolytic enzyme activities statistical chart of embodiment 1；
Fig. 6 is the test pig intestinal fat enzymatic activity statistical chart of embodiment 1；
Fig. 7 is the test pig Amylase Activity statistical chart of embodiment 1；
Fig. 8 is the test pig enteron aisle tryptic activity statistical chart of embodiment 1；
Fig. 9 is the test pig enteron aisle mucus IgA content statistical chart of embodiment 1.
Specific embodiment
It in order to enable those skilled in the art to better understand the solution of the present invention, below will be to the skill in the embodiment of the present invention
Art scheme is clearly and completely described, it is clear that and the described embodiment is only a part of the embodiment of the present invention, without
It is whole embodiments.
Embodiment 1
Three test groups, including a control treatment and two processing groups are arranged in the present embodiment, carry out weaning pig feeding
Test, wherein processing I and processing II are the feeding experiment pig in the pig house for being equipped with fermentation bed, and control treatment is traditional
Feeding experiment pig in cement floor pig house.
1. making fermenting bed padding
Processing I and processing II prepare fermenting bed padding and use fermentation substrate identical, raw material needed for the fermentation substrate and its again
It is as shown in table 1 to measure number, according to parts by weight cited by table 1, weighs various required raw materials in proportion.
Raw material of the processing of table 1 I for fermenting bed padding forms
In addition to above-mentioned fermentation substrate, the raw material that the processing I of the present embodiment prepares fermenting bed padding further includes 3 parts of fermenting agent
With 5 parts of brown sugar.Effective total viable count >=10 in the fermenting agent8Cfu/g, effective viable bacteria is by bacillus cereus, lichens
Bacillus, clostridium butyricum, saccharomycete and lactic acid bacteria composition, viable count therein is than meeting: bacillus cereus: lichens gemma
Bacillus: clostridium butyricum: saccharomycete and lactic acid bacteria=0.75:0.75:1.5:1:2.
The fermenting bed padding that processing I uses is prepared in accordance with the following steps:
(1) above-mentioned fermenting agent is added brown sugar water activation culture 24 hours with the amount of 5wt%, obtains zymocyte liquid；
It (2) will weighed bagasse, vinegar grain, needle mushroom mushroom bran, oyster mushroom mushroom bran, cotton seed hulls and phosphoric acid hydrogen according to parts by weight
Calcium mixing, is stirred well to and is uniformly distributed, and obtains fermentation substrate, C/N=59:1, the carbon phosphorus of the fermentation substrate that the present embodiment is prepared
Compare C/P=86:1；
(3) zymocyte liquid that activation is sprayed into fermentation substrate stirs fermentation substrate while spraying；
(4) after the inoculation fermentation bacterium solution into fermentation substrate, add the water content of water adjusting fermentation substrate to 58%；
(5) heap fermentation 7 days.
The fermenting bed padding that processing II uses is prepared in accordance with the following steps:
It (1) will weighed bagasse, vinegar grain, needle mushroom mushroom bran, oyster mushroom mushroom bran, cotton seed hulls and phosphoric acid hydrogen according to parts by weight
Calcium mixing, is stirred well to and is uniformly distributed, and obtains fermentation substrate, C/N=59:1, the carbon phosphorus of the fermentation substrate that the present embodiment is prepared
Compare C/P=86:1；
(2) add the water content of water adjusting fermentation substrate to 58%；
(3) heap fermentation 7 days.
2. constructing fermentation bed
Adding water to adjust fermenting bed padding to the water content for completing to ferment is 40%, utilizes acidity-basicity regulator adjustment fermentation bed
The pH value of padding is to pH=7.2.Fermenting bed padding Jing Guo above-mentioned processing is spread out in culturing area, laying with a thickness of
70cm, next day is into pig.
3. feeding experiment
It is " Ternary Pig " the three way cross weanling pig 30 of 7.5kg or so that test pig, which selects weight, and parity is consistent, product
Kind is identical, age in days is close, according to the fifty-fifty group technology of male and female, is randomly divided into 3 groups, every group of 10 repetitions, each repetition 1 is tried
Test pig.Test pig house sets 7 days preliminary trial periods before into Antiepidemic sterilizing work is carried out before pig, testing formal start, and during prerun, presses
The work such as vaccine injection, expelling parasite and earmarking are carried out to test pig according to farm's normal feeding management process.Experimental period is 42
It, during test: the test pig of control treatment is freely eaten in cement floor pig house, drinks water, and does clear mode and cleans excrement；Place
Reason I and the test pig of processing II are freely eaten in fermentation bed, drink water and defecation, daily ration used by three groups of test pigs of feeding
Composition is as shown in table 2.
The daily ration of 2 test pig of table forms
It in experimental period, turns over take off II fermentation bed surface layer padding of processing I and processing daily, make the fecaluria for being in padding surface layer originally
It is covered and buries by padding, turn over comprehensively take off after the 50cm of upper layer that monthly thoroughly to plough deeply padding primary weekly.Whenever padding height decline about 10cm,
The fermenting bed padding supplement of same composition is added, when charging, virgin material and old material will be sufficiently mixed to reach strain and be uniformly dispersed, together
When regulate moisture content to 40%.
4. slaughter experiment
After feeding experiment, at random from every group it is each repeat selective body weight similar in each 2 of sodium selenite (male and female is each
Half), 4% Nembutal sodium solution of intramuscular injection (40mg/kg) is anaesthetized.After anaesthetizing completely, abdominal cavity is cut, carries out butchering survey
It is fixed.Prohibit before killing and raise for 24 hours, prohibits free water during raising, weighing before killing.
5. test index
The acquisition and preservation of 5.1 samples
The acquisition of blood sample and food preservation test terminate same day 8:00, all test pig vena cava anteriors blood sampling about 10mL, system
Standby 2 parts of blood samples, 1 part adds heparin sodium to prepare whole blood；1 part of 3000r/min centrifugation 15min prepares serum, and -70 DEG C of preservations are used for
Index of correlation measurement.The preceding 1 day 20:00 that takes a blood sample stops being fed with grain, makes test pig empty stomach 12h.
After butchering, sterile working ligatures duodenum, colon and caecal content in every weanling pig same area immediately
Object about 2g sterilizes each ligation mouth with cotton ball soaked in alcohol, is put into disinfecting container, 4 DEG C are transported back laboratory immediately.In sterile room,
Content and physiological saline are sufficiently mixed respectively, to microorganism in intestinal contents (Escherichia coli, salmonella, cream
Acidfast bacilli, Bifidobacterium) it is detected by light Gang Shi intestinal flora analysis method.
The measurement of 5.2 production performances and diarrhea rate
Feed consumption rate and test pig total diarrhea head number daily are recorded during test daily, respectively at the formal beginning and end of test
When, for 24 hours, weigh in the morning 8:00 on an empty stomach for fasting (free water).By the per day feed intake of every group of calculating test pig, averagely increase day by day
Weight, feed-weight ratio and diarrhea rate:
Average daily gain (ADG)=(last weight-initial weight)/number of days；
Average daily gain (ADFI)=total feed consumption amount/number of days；
Feed-weight ratio (F/G)=feed consumption rate/weight gain；
Diarrhea rate (%)=(diarrhea piglet head number × grice diarrhoea number of days)/(test piglet head number × just try number of days) ×
100%.
The measurement of 5.3 intestinal floras
Colon, cecal content 0.5g are taken in superclean bench, is put into sterile test tube, and sterile PBS dilution is added
4.5mL shakes 3-5min in magnetic force oscillator, this liquid is 10- 1Dilution.Then take this dilution 0.5mL in another sterile examination
In pipe, 4.5mL sterile diluent is added and carries out 10- 2Dilution, after concussion, then successively carries out 10- 3–10- 6It dilutes again.Respectively will not
Same intestinal segment, different dilution 0.1mL dilution be applied on each identification culture medium.Bacillus acidi lactici and Bifidobacterium are inoculated in respectively
In MRS and Bifidobacterium selectivity (BS) culture medium, after 37 DEG C of 48-72h of Anaerobic culturel, bacterium colony meter is carried out using colony counting method
Number.Escherichia coli, salmonella respectively in Yihong methylene blue (EMB), shiga-Salmonella agar medium (SS) 37 DEG C have
Oxygen culture 18~carry out bacterium colony counting afterwards for 24 hours.As a result with logarithm (logCFU/g) table of bacterium number in every gram of intestinal contents
Show.Each dilution sets 3 repetitions.It is identified according to the methods of flora cellular morphology, Gram's staining, oxygen-consuming these types of thin
Bacterium.
The measurement of 5.4 digestive enzyme activities
The duodenum content of 0.2g is weighed, the cold physiological saline of 4.0mL is added, is homogenized in ice-water bath, 4 DEG C,
16000g is centrifuged 15min, takes supernatant for measuring the various digestive enzyme activities of duodenum content.
Amylase activity measurement: iodine-starch colorimetric method is used.Unit of enzyme activity is defined as: in 1g duodenum content
Amylase, in 37 DEG C and substrate-function 30min, hydrolysis 10mg starch is 1 liquefon (U).
Determination of tryptic activity: N- Benzoyl-L-arginine ethyl ester (BAEE) method is used.Unit of enzyme activity is defined as:
Under the conditions of 8.0,37 DEG C of pH, the trypsase contained in 1mL intestinal contents makes D253 value increase 0.003 1 enzymes per minute
Active unit (U).
The active measurement of total protease: forint- phenol law is used.Unit of enzyme activity is defined as: in 40 DEG C, 7.0 condition of pH
Under, enzyme amount needed for caseinhydrolysate per minute generates 1 μ g tyrosine is 1 pancreas total protein hydrolytic enzyme activities unit (U).
Lipase activity determination: turbidimetry for Determination is used.Unit of enzyme activity is defined as: 1min is acted in 40 DEG C of water-baths,
Enzyme amount needed for hydrolyzing 1 μm of ol matrix (olive oil) is 1 unit of lipase activity (U).
5.5 test on immune function
Sampling is butchered when off-test, takes jejunum starting point to ileocecal sphincter intestinal tube, it is slow with cold distilled water and phosphate
Fliud flushing (PBS, pH=7.2) is successively laid on filter paper after flow wash, is longitudinally cut off, and exposure mucous membrane face, 4 DEG C of PBS are rinsed, and is used
Cleaning glass slide gently scrapes intestinal mucosa surface mucus, collects scraping object 0.5mL with micro syringe, then uses 4.5mL PBS
It dilutes and carefully stirs, make it completely dissolved, 4 DEG C of centrifugation 5min, collect supernatant, 4 DEG C of storages are spare, glutinous for measuring enteron aisle
The content of IgA in liquid.IgA content builds up the Immunoglobulin reagents box of Bioengineering Research Institute's production using Nanjing, in RX
daytonaTMIt is measured on automatic clinical chemistry analyzer.At the end of experimental period, all the participate in the experiment blood sampling of pig vena cava anterior and smears are used
Non-specific acidity α-acetic acid how esterase staining and methyl green-pyrrole sieve red colouring method measurement T lymphocyte and bone-marrow-derived lymphocyte,
Calculate separately Activated T-lymphocytes, bone-marrow-derived lymphocyte accounts for the percentage of total lymphocyte.
6. test result
6.1 growth performance test results
Three groups of growth performance test results for trying test pig are as shown in table 3, at the end of experimental period, each processing group test
The weight size relation of pig are as follows: I > of processing handles II > control treatment, and for feed-weight ratio, processing I is substantially less than at control
Reason.The 5th day after entering experimental period, start symptom of diarrhea occur in the test pig of control treatment, at the end of experimental period, according to
It is so found diarrhea test pig, has 8 diarrhea test pigs in entire experimental period altogether.In comparison, processing I and processing II
The diarrhea phenomenon of test pig significantly mitigate: the test pig for handling I starts symptom of diarrhea occur on the 6th day after entering experimental period,
And to entering after experimental period the 12nd day until experimental period terminates have diarrhea test pig without discovery, go out altogether in entire experimental period
Existing 2 diarrhea test pigs；The test pig of processing II starts symptom of diarrhea occur on the 5th day after entering experimental period, has arrived entrance
Terminate have diarrhea test pig without discovery to experimental period within the 20th day after experimental period, has 4 diarrhea in entire experimental period altogether
Test pig.In experimental period, three groups of test pigs for the state of mind of each group test pig, handle I He without there is dead example
The test pig of processing II is considerably better.It is according to the diarrhea rate score listed in table 3 it may be said that bright, pig is raised using fermentation bed,
It can be effectively reduced the diarrhea rate of weanling pig, reduce after weanling pig wean to feed daily ration as stress be anti-caused by food
It answers.The feeding effect of comparison processing I and processing II adds fermenting agent in fermentation substrate and prepares fermenting bed padding, is conducive to
Organic substance in fermentation substrate is converted into test pig growth needed nutrient matter, and test pig, can by arch food fermenting bed padding
Further to promote the growth and development of test pig, reduce the feed-weight ratio of test pig.
Growth performance test result of 3 the present embodiment of table for examination test pig
The measurement of 6.2 intestinal floras
For the intestinal flora measurement result of each group test pig as shown in Fig. 1-4, processing I and processing II significantly reduce test pig
The quantity of Escherichia coli and salmonella in colon and caecum, significantly improves the quantity of Bacillus acidi lactici and Bifidobacterium, and right
Compare according to processing: processing I, Escherichia coli quantity reduces 25.07% and 22.44% respectively in colon and caecum, colon and blind
Salmonella quantity reduces 14.01% and 20.10% respectively in intestines, and Bacillus acidi lactici quantity increases respectively in colon and caecum
10.75% and 11.22%, bifidobacteria increases 11.29% and 11.02% respectively in colon and caecum；Processing II, knot
Escherichia coli quantity reduces 11.36% and 6.89% respectively in intestines and caecum, and salmonella quantity is distinguished in colon and caecum
Reduce 5.80% and 6.70%, Bacillus acidi lactici quantity increases 2.97% and 3.12% respectively in colon and caecum, colon and
Bifidobacteria increases 3.31% and 2.69% respectively in caecum.Illustrate the fermentation bed using II preparation of processing I and processing
Padding raises pig, can adjust the microbial population of pig enteron aisle, certain facilitation be played to probiotics, to pathogenic bacteria
Play certain inhibiting effect.
6.3 digestive enzyme activity measurement results
As shown in the result of Fig. 5-8, compared with control treatment, the test pig duodenum content of processing I and processing II
Total protein hydrolase, lipase, amylase and trypsase all obtained certain raising: the total protein water of I sample of processing
Solution enzyme, lipase, amylase, tryptic activity have been respectively increased 5.07%, 34.26%, 4.83%, 14.67%；Processing II
Total protein hydrolase, lipase, amylase, the tryptic activity of sample be respectively increased 1.58%, 14.14%, 1.54%,
11.13%.
6.4 test on immune function results
As shown in table 4, compared with the test pig of control treatment, peripheral blood T, B leaching of the test pig of processing I and processing II
Bar cell positive rate is significantly raised.As shown in figure 9, compared with the test pig of control treatment, the test pig of processing I and processing II
IgA content in enteron aisle mucus is also significantly improved.
The peripheral blood T of 4 the present embodiment test pig of table, bone-marrow-derived lymphocyte positive rate (%) statistical result
Finally it should be noted that the above embodiments are merely illustrative of the technical scheme of the present invention and are not intended to be limiting thereof, to the greatest extent
Invention is explained in detail referring to above-described embodiment for pipe, it should be understood by a person of ordinary skill in the art that technology
Personnel read present specification after still can with modifications or equivalent substitutions are made to specific embodiments of the invention, but this
A little modifications are changed within all without departing from the present patent application accompanying claims protection scope.
Claims (10)
1. a kind of weanling pig grows fermenting equalizing bed padding, using fermenting agent and fermentation substrate as raw material, by heap
Product fermentation preparation, it is characterised in that:
The fermentation substrate includes following parts by weight material: 10-20 parts of bagasse, 10-20 parts of vinegar grain, edible 40-60 parts of mushroom bran,
10-20 parts of cotton seed hulls；
The parts by weight of the fermenting agent are 1-5 part, living bacteria count >=10 in the fermenting agent8Cfu/g, it is therein
Efficient bacterium group is combined into bacillus+saccharomycete+lactic acid bacteria, and meets following living bacteria count ratio, the bacillus: described
Saccharomycete: the lactic acid bacteria=1-5:0.5-1.5:1-3.
2. weanling pig as described in claim 1 grows fermenting equalizing bed padding, which is characterized in that in the fermenting agent
Living bacteria count meets: the bacillus: the saccharomycete: the lactic acid bacteria=3:1:2.
3. weanling pig as described in claim 1 grows fermenting equalizing bed padding, it is characterised in that: the bacillus is selected from
Bacillus subtilis, bacillus licheniformis, bacillus coagulans, bacillus cereus, bacillus pumilus, in clostridium butyricum
It is one or more.
4. weanling pig as claimed in claim 3 grows fermenting equalizing bed padding, it is characterised in that: the bacillus is by institute
Bacillus cereus, bacillus licheniformis and clostridium butyricum composition are stated, and meets following living bacteria count ratio: the waxy gemma
Bacillus: the bacillus licheniformis: the clostridium butyricum=1:1:2.
5. weanling pig as described in claim 1 grows fermenting equalizing bed padding, it is characterised in that: the edible mushroom bran includes
Needle mushroom mushroom bran peace mushroom chaff.
6. weanling pig as claimed in claim 5 grows fermenting equalizing bed padding, it is characterised in that: according to parts by weight, institute
Edible mushroom bran is stated to be made of 30 parts of needle mushroom mushroom brans and 25 parts of oyster mushroom mushroom brans.
7. weanling pig as described in claim 1 grows fermenting equalizing bed padding, it is characterised in that: the fermentation substrate includes
15 parts of the vinegar grain, 50 parts of the edible mushroom bran, 3 parts of the fermenting agent, 0-10 part of calcium monohydrogen phosphate, the raw material further includes red
3-5 parts of sugar.
8. the preparation method of weanling pig growth fermenting equalizing bed padding, feature exist as described in any one of claim 1-7
In, comprising the following steps:
The fermenting agent is added 20-30h of activation culture in brown sugar water with the amount of 5wt%, obtains activation fermentation by step (1)
Bacterium solution；
Step (2), material needed for weighing the fermentation substrate stir and evenly mix, and obtain the fermentation substrate；
The activation zymocyte liquid is inoculated with the fermentation substrate by step (3)；
Step (4) adds water to adjust the fermentation substrate, makes the water content 55-65% of the fermentation substrate；
Step (5), heap fermentation 6-8 days.
9. the preparation method of weanling pig growth fermenting equalizing bed padding as claimed in claim 8, it is characterised in that: described
In step (2), the parts by weight of the bagasse, the cotton seed hulls and the calcium monohydrogen phosphate are controlled, make the fermentation substrate
Carbon-nitrogen ratio C/N=40-60:1, carbon-phosphorus ratio C/P=80-140:1.
10. the preparation method of weanling pig growth fermenting equalizing bed padding as claimed in claim 8, it is characterised in that: in institute
It states in step (4), after the water content adjusting for completing the fermentation substrate, adjusts the pH value of the fermentation substrate, make its pH=
6–8。
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| CN111602629A (en) * | 2020-05-29 | 2020-09-01 | 南宁学院 | Breeding method for improving growth performance of broiler chickens by using fermented Chinese herbal medicines |
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