CN101113412B - Method for preparing multi-purpose compound fungus - Google Patents
Method for preparing multi-purpose compound fungus Download PDFInfo
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- CN101113412B CN101113412B CN2007100546773A CN200710054677A CN101113412B CN 101113412 B CN101113412 B CN 101113412B CN 2007100546773 A CN2007100546773 A CN 2007100546773A CN 200710054677 A CN200710054677 A CN 200710054677A CN 101113412 B CN101113412 B CN 101113412B
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Abstract
The invention relates to a preparation method of a multipurpose compound mildew, aiming at solving the problem of the requirements of beneficial mildew during production. The technic proposal solved by the invention is that: edible fiber fungus, beer budding fungus and streptomyces rimosus are respectively mixed into a culture medium in a cultivating vessel according to a matching ratio and cultivated for a certain time at favorable temperature and pH value, and respectively put into three seed cans for anaerobic fermentation under certain temperature, then the three thalli are mixed togetherby proportion after the fermentation, and wheat bran or rice polishings are added by proportion to expand the propagation, then second fermentation is carried out after propagation expansion and the mixture is dried to get bacterial powder, finally the powder is packed and stored in a warehouse. The method of the invention is simple with good cultivating effect and the mildew has wide use and high value, thereby having wide development and application prospect and great economic benefit.
Description
One, technical field
The present invention relates to microorganism field, particularly a kind of preparation method of multi-purpose compound fungus.
Two, technical background
At present, developed country's using microbe technology in agriculture production is very extensive, and scientific and technological content is very high.As enzymatic microorganism, EM, ten thousand fields of Japan three famous brands of fermenting, the characteristics of these microorganism species are: many bacterial classifications, multi-functional can be widely used in producing fertilizer, microbial pesticide, feed, healthcare products or sewage disposal.And China is used for the bacterial classification that microorganism fertilizer is produced, and kind is few, and purposes is few, and consumption is few, and DeGrain as vinelandii, microorganism potassium, the microbe-P of various brands, comprises the A Musi of maximum brand, and these probiotics quantity are all fewer.Therefore, researching and developing many bacterial classifications, multi-functional, multiduty beneficial microbe colony, is the key subjects that China scientific and technological circle face.
The agricultural microorganism has broad application prospects in fields such as proportion of crop planting industry, its feeding industry, culture fishery, processing of farm products, agriculture environmental protection and agriculture production technologies.Developed country's using microbe technology in agriculture production is very extensive at present; from the application development of past single culture to composite flora; can be widely used in and produce biological organic fertilizer, biological pesticide and biological fodder, can be used for agricultural products fresh-keeping or be used for environment protection (sewage rubbish processing).China's present agriculture microorganism storehouse preservation bacterial classification kind more than 3000, and beneficial microorganism 2003 statistics that is used for bio-feritlizer kind more than 80, few in particular for the bacterial classification kind of fertiliser production, the bacterium amount is few.China utilizes the microorganisms producing bio-organic fertilizer just to begin starting, also very backward, along with the actual needs in development of science and technology and the production, have 300 tame biological organic fertilizer products in Ministry of Agriculture's registration, production by the end of the year ends 2003, also have many registrations of declaring, total amount surpasses 3,500,000 tons, is 8 times of nineteen ninety-five.Find out that from above the application development impetus of agriculture microorganism on organic fertilizer is gratifying, situation is also pressing, rising greatly, but, for various reasons, the present beneficial microorganism breeding amount of China is little, can not satisfy the actual needs in the production, therefore, to cultivate breeding be the problem that solves of making great efforts to beneficial microorganism.
Three, summary of the invention
At above-mentioned situation, the present invention's purpose just provides a kind of preparation method of multi-usage compound fungus, with in solve producing to the problem that needs of beneficial moulds, the technical scheme of its solution is, to have a liking for the fiber bacterium, saccharomyces cerevisiae, streptomyces rimosus mixes separately training by proportioning respectively and grows in the substratum in the device, under suitable temperature and pH value, cultivate certain hour, insert respectively again in three seeding tanks, carry out anaerobically fermenting at a certain temperature, after the fermentation three kinds of thalline are mixed together in proportion, add wheat bran or rice bran more in proportion and expand numerous, expand and carry out Secondary Fermentation after numerous, dry back becomes strain powder, the packing of weighing warehouse-in, and the inventive method is simple, training is grown effective, its mould purposes is wide, is worth greatly, and wide development application prospect and huge economic benefit are arranged.
Four, embodiment
Bacterial classification of the present invention is commercially available or has a liking for the fiber bacterium from the relevant Chinese Academy of Sciences introduced, saccharomyces cerevisiae and streptomyces rimosus, at first do female the kind with three kinds of bacterium, three kinds of female bacterial classifications are expanded grow cultivation, step is, carry out the routine sterilization to having a liking for the fiber bacterium culture medium, to have a liking for fiber starter kind again inserts and the training of having a liking for the fiber bacterium culture medium is housed grows in the test tube, the bacterial classification add-on is 1: 100 by bacterial classification to the substratum weight ratio, having a liking for the every 1000g of fiber bacterium culture medium (solid) is by extractum carnis 4-15g, peptone 15-20g, Zulkovsky starch 1-3g, salt 6-8g, agar 18-20g and surplus are that water is formed, after having a liking for fiber starter kind implantation substratum, 26 ℃ of constant temperature, pH value is that 7.3 times trainings were grown 84 hours, breed into the test tube kind, insert again to train to grow and carry out anaerobically fermenting in the jar, stand-by at 18-30 ℃ of following anaerobically fermenting 200 hours (about 8.5 days, i.e. 9 daytimes of 8 nights); The cereuisiae fermentum bacterium culture medium is carried out the routine sterilization, by weight saccharomyces cerevisiae is that 1: 100 ratio is implanted saccharomyces cerevisiae than cereuisiae fermentum bacterium culture medium after the sterilization, shake-flask culture is 84 hours under 26 ℃ of constant temperature, numerous saccharomyces cerevisiae test tube kind that expands into, said cereuisiae fermentum bacterium culture medium transfers to 1000ml by glucose 25-35g, yeast powder 15-25g with fermented bean drink and forms, the saccharomyces cerevisiae test tube kind that expands numerous one-tenth insert again training grow jar in 18-30 ℃ of following anaerobically fermenting 200 hours, become and expand the saccharomyces cerevisiae of growing, standby; The streptomyces rimosus substratum is carried out 120 ℃ of high pressure moist heat sterilizations, the training that the female kind of streptomyces rimosus is inserted the streptomyces rimosus substratum that the bacterium of going out is housed is grown in the jar again, the female kind of streptomyces rimosus add-on is 1/100 of a substratum weight, be that weight ratio is 1: 100, pH value is 7.3, constant temperature is trained down for 26 ℃ and was grown 84 hours, breed into the test tube kind, said streptomyces rimosus substratum is that the substratum of every 1000ml (liquid) is by Zulkovsky starch 15-25g, saltpetre 0.5-1.5g, salt (sodium-chlor) 0.4-0.8g, K
2HPO
43H
2O (dipotassium hydrogen phosphate) 0.4-0.8g, MgSO
47H
2O (sal epsom is commonly called as Epsom salts) 0.4-0.8g, FeSO
47H
2O (ferrous sulfate, being commonly called as green vitriol) 0.005-0.02g, agar 15-25g and surplus be that water is made, pH value is 7.2-7.4, during preparation, earlier with cold water with Zulkovsky starch furnishing pasty state, pour into again in the water that boils, on fire, heat, add other compositions while stirring, fully after the dissolving evenly, supply moisture to 1000ml, the test tube kind streptomyces rimosus of breeding is inserted training again and is grown in the jar, under 18-30 ℃ temperature, carried out anaerobically fermenting 200 hours (about 8.5 days, i.e. 9 daytimes of 8 nights), the streptomyces rimosus that expansion is grown is grown in training, and is standby; Three kinds are expanded have a liking for fiber bacterium, saccharomyces cerevisiae and the streptomyces rimosus grow and are mixed with 1: 1: 1 ratio of weight and form mixed bacterium together behind anaerobically fermenting, insert in the culture tank again, adding wheat bran or rice bran expands numerous, carry out solid and expanded numerous 240-300 hour under 15-30 ℃, the weight ratio of wheat bran or rice bran and mixed bacterium is 100: 1, puts into training again after the solid expansion is numerous and grows jar, 25 ℃ of constant temperature bottom fermentations 200 hours, (times in promptly about 9 daytimes of 8 nights), become bacterium powder, water content 2.5-4% behind the drying and dehydrating.
The present invention also can be by following specific embodiment preparation.
At first do female the kind with three kinds of bacterium, three kinds of female bacterial classifications are expanded grow cultivation, step is, carry out the routine sterilization to having a liking for the fiber bacterium culture medium, to have a liking for fiber starter kind inserts and the training of having a liking for the fiber bacterium culture medium is housed grows in the test tube, every 100g has a liking in the fiber bacterium culture medium and to add 1g and have a liking for the fiber bacterial classification, the every 1000g of substratum (solid) is by extractum carnis 5g, peptone 10g, Zulkovsky starch 2g, salt 6g, agar 19g and surplus are that water is formed, after having a liking for fiber starter kind implantation substratum, 26 ℃ of constant temperature, pH value is that 7.3 times trainings were grown 84 hours, breed into the test tube kind, insert training again and grow and carry out anaerobically fermenting in the jar, stand-by 26 ℃ of following anaerobically fermentings 200 hours; The cereuisiae fermentum bacterium culture medium is carried out the routine sterilization, saccharomyces cerevisiae after the sterilization adds in the microzyme culture medium, add cereuisiae fermentum bacterial classification 1g in every 100ml substratum, shake-flask culture is 84 hours under 26 ℃ of constant temperature, numerous saccharomyces cerevisiae test tube kind that expands into, said cereuisiae fermentum bacterium culture medium transfers to 1000ml by glucose 30g, yeast powder 20g with fermented bean drink and forms, the saccharomyces cerevisiae test tube kind that expands numerous one-tenth insert again training grow jar in 26 ℃ of following anaerobically fermentings 200 hours, become to expand the saccharomyces cerevisiae of growing, standby; The streptomyces rimosus substratum is carried out 120 ℃ of high pressure moist heat sterilizations, the training that the female kind of streptomyces rimosus is inserted the streptomyces rimosus substratum that the bacterium of going out is housed is grown in the jar, add 1g streptomyces rimosus kind in every 100ml streptomyces rimosus substratum, promptly 1: 100, pH value is 7.3, constant temperature is trained down for 26 ℃ and was grown 84 hours, breeds into the test tube kind, and said streptomyces rimosus substratum is that the substratum of every 1000ml (liquid) is by Zulkovsky starch 20g, saltpetre 1g, salt (sodium-chlor) 0.5g, K
2HPO
43H
2O (dipotassium hydrogen phosphate) 0.5g, MgSO
47H
2O (sal epsom is commonly called as Epsom salts) 0.5g, FeSO
47H
2O (ferrous sulfate is commonly called as green vitriol) 0.01g, agar 20g and surplus are that water is made, and pH value is 7.3, during preparation, elder generation, pours in the water that boils Zulkovsky starch furnishing pasty state with cold water again, on fire, heat, add other compositions while stirring, fully after the dissolving evenly, supply moisture to 1000ml, the test tube kind streptomyces rimosus of breeding is inserted training again and is grown in the jar, under 18-30 ℃ temperature, carries out anaerobically fermenting 200 hours, the streptomyces rimosus that expansion is grown is grown in training, and is standby; Three kinds are expanded have a liking for fiber bacterium, saccharomyces cerevisiae and the streptomyces rimosus grow and are mixed with 1: 1: 1 ratio of weight and form mixed bacterium together behind anaerobically fermenting, insert in the culture tank again, adding wheat bran or rice bran expands numerous, carrying out solid under 18-28 ℃ expanded numerous 240 hours, add mixed bacterium 1g in every 100g wheat bran or the rice bran, solid expands to be put into training again after numerous and grows jar, 25 ℃ of constant temperature bottom fermentations 200 hours, become bacterium powder, water content 3% behind the drying and dehydrating.
One, the compound fungus grown of the present invention training is used to produce fertilizer and not only contains not nitrogen, phosphorus, potassium, trace element, but also contain plurality of enzymes such as multivitamin, amino acid, saccharide compound, lipoid substance, Mierocrystalline cellulose, nucleic acid and SOD, but chessom, culture fertility, preserve moisture and fertility, improve soil CEC value, reduce the EC value, improve ground temperature 2-5 degree, well developed root system, it is healthy and strong to grow, and percentage of fertile fruit improves more than 25%, and ripen in advance is about 15 days; Keep soil suitable substance P H value, the effect in improvement saltings is arranged; Reduce disease and pest and take place, reduce chemical pesticide consumption and number of times; Improve crop quality, reduce the agricultural-food nitrate content greatly, pol improves 1-3 degree, storage endurance; Farm crop are improved output more than 25%, reduce cost more than 30%.
Two, compound fungus fermented tcm of the present invention is made farming sterilant, sterilant.
Obtained positive effect with compound fungus fermented tcm making sterilant of the present invention, poison insecticide.Medicament in the microbial fermentation, by materials such as the organic acid of metabolism generation, pure and mild enzymes, ore deposit element in the dissolving Chinese medicine, sterilization components such as the vegeto-alkali in decomposition, the conversion Chinese medicine, flavonoid, the rare class of terpene, improve drug effect more than 35 times, add tens kinds of antibiotic that metabolism produces, can improve the prevention and elimination of disease and pests effect greatly, the plant sickness rate reduces more than 50%.
Traditional Chinese medicine bactericide, sterilant can also the fiting chemical agricultural chemicals use, and not only can reduce half pesticide dosage, can also increase drug effect, and spray back 3 days just can degrading pesticide effect, significantly reduce the residual quantity of agricultural chemicals in agricultural-food.
Three, make fodder additives with compound fungus
Promote appetite of livestock and poultry, help digest, feed by fermentation has good sweet taste and wine flavour, can improve a poor appetite.Enzyme and organic acid that fermentation is decomposed can promote digesting and assimilating of various nutrition, and be high as the albumen transformation efficiency, and domestic animal grows up fast, just delivered for sale in individual month as pig 8-10, edible.
Promote to produce, improve meat.In the secondary metabolism of compound fungus, can produce the factor of multiple stimulation animal growth, both can promote the metabolism of fat, can prevent again owing to the phenomenon that meat descends takes place adipopexis, improve lean ratio, the feedstuff-meat ratio of raising pigs is about 3: 1 or 4: 1, and the pig body weight increases day by day more than the 0.65kg.Raise chickens and can prolong laying period, improve laying rate about 15%, broken rate of eggshell drops to 0.3%, and soft egg reduces to 1%.Milk cow can be improved the rate of giving milk 20%, average day volume increase fresh milk 2-4kg.Improve the disease resistance of livestock and poultry, do not re-use western countries' forbidding microbiotic and hormone, save expenses for medicine, reduce investment 40%, only carry out necessary epidemic preventing working and get final product.Reduce the ight soil stench, improve breeding environment.The feces of livestock and poultry quality is good, and being used for kind of value is good fertilizer, and feed cost reduces more than 25%, and technology is eager to learn, grasps easily.
Four, environment protection aspect
Be used for sewage deodorizing: domestic refuse is made fertilizer, make household cleaning agent.
Five, be used for body-care, make beverage, fermented tcm is used for medical treatment
The body-building and the mechanism of curing the disease, be the Chinese medicinal ingredients of fermentation conversion and the polyphenoils of generation, as: VITAMIN, microbiotic, amino acid, ore deposit element, plurality of enzymes and nucleic acid, and cancer-resisting substance such as dehydroepiandros-sterone, genistein, allow the profitable strain in the stomach have comparative advantage, eliminate the harm of harmful bacterium and oxyradical in the body, remove disease and old and feeble root, thereby the recovery raise immunity takes a turn for the better or rehabilitation the human body various diseases.
Six, compound fungus is not only high in technological content, and price is low
The every gram of compound fungus contains probiotics 〉=1,000,000,000, contain 600,000,000 of bacterium considerably beyond the every gram of Japanese enzymatic microorganism, function is many, is higher than Japanese enzymatic microorganism as nitrogen fixing capacity, how the gene induced regulatory expression technique of plant phenotype than Japan, can improve the cold tolerance of farm crop greatly.With 1 ton of fertilizer of Japanese enzymatic microorganism fermentation need bacterium we 20 kilograms, 1 ton of fertilizer of compound fungus fermentation, only need the 4-8 kilogram, 1 ton of Japanese enzymatic microorganism of import needs 250,000 yuans, and only 100,000 yuans of the prices of 1 ton of former bacterial classification of compound fungus, cost reduces more than 70%, and non-environmental-pollution, and the society and economy benefit is huge.
Claims (2)
1. the preparation method of a multi-purpose compound fungus, it is characterized in that, earlier to have a liking for the fiber bacterium, saccharomyces cerevisiae and streptomyces rimosus are done female the kind, three kinds of female bacterial classifications are expanded grow cultivation, step is, carry out the routine sterilization to having a liking for the fiber bacterium culture medium, to have a liking for fiber starter kind again inserts and the training of having a liking for the fiber bacterium culture medium is housed grows in the test tube, the bacterial classification add-on is 1: 100 by bacterial classification to the substratum weight ratio, having a liking for the every 1000g of fiber bacterium culture medium is by extractum carnis 4-15g, peptone 15-20g, Zulkovsky starch 1-3g, salt 6-8g, agar 18-20g and surplus are that water is formed, after having a liking for fiber starter kind implantation substratum, 26 ℃ of constant temperature, pH value is that 7.3 times trainings were grown 84 hours, breed into the test tube kind, insert training again and grow and carry out anaerobically fermenting in the jar, stand-by 18-30 ℃ of following anaerobically fermenting 200 hours; The cereuisiae fermentum bacterium culture medium is carried out the routine sterilization, by weight saccharomyces cerevisiae is that 1: 100 ratio is implanted saccharomyces cerevisiae than cereuisiae fermentum bacterium culture medium after the sterilization, shake-flask culture is 84 hours under 26 ℃ of constant temperature, numerous saccharomyces cerevisiae test tube kind that expands into, said cereuisiae fermentum bacterium culture medium transfers to 1000ml by glucose 25-35g, yeast powder 15-25g with fermented bean drink and forms, the saccharomyces cerevisiae test tube kind that expands numerous one-tenth insert again training grow jar in 18-30 ℃ of following anaerobically fermenting 200 hours, become and expand the saccharomyces cerevisiae of growing, standby; The streptomyces rimosus substratum is carried out 120 ℃ of high pressure moist heat sterilizations, the training that the female kind of streptomyces rimosus is inserted the streptomyces rimosus substratum that the bacterium of going out is housed is grown in the jar again, the female kind of streptomyces rimosus add-on is 1/100 of a substratum weight, be that weight ratio is 1: 100, pH value is 7.3, constant temperature is trained down for 26 ℃ and was grown 84 hours, breeds into the test tube kind, and the substratum that said streptomyces rimosus substratum is every 1000ml is by Zulkovsky starch 15-25g, saltpetre 0.5-1.5g, salt 0.4-0.8g, K
2HPO
43H
2O 0.4-0.8g, MgSO
47H
2O0.4-0.8g, FeSO
47H
2O 0.005-0.02g, agar 15-25g and surplus are that water is made, and pH value is 7.2-7.4, during preparation, elder generation, pours in the water that boils Zulkovsky starch furnishing pasty state with cold water again, heats on fire, add other compositions while stirring, fully after the dissolving evenly, supply moisture to 1000ml, the test tube kind streptomyces rimosus of breeding is inserted training again and is grown in the jar, under 18-30 ℃ temperature, carried out anaerobically fermenting 200 hours, the streptomyces rimosus that expansion is grown is grown in training, and is standby; Three kinds are expanded have a liking for fiber bacterium, saccharomyces cerevisiae and the streptomyces rimosus grow and are mixed with 1: 1: 1 ratio of weight and form mixed bacterium together behind anaerobically fermenting, insert in the culture tank again, adding wheat bran or rice bran expands numerous, carrying out solid under 15-30 ℃ expanded numerous 240-300 hour, the weight ratio of wheat bran or rice bran and mixed bacterium was 100: 1, and solid expands to be put into training again after numerous and grow jar, 25 ℃ of constant temperature bottom fermentations 200 hours, become bacterium powder, water content 2.5-4% behind the drying and dehydrating.
2. the preparation method of multi-purpose compound fungus according to claim 1, it is characterized in that, at first to have a liking for the fiber bacterium, saccharomyces cerevisiae and streptomyces rimosus are done female the kind, three kinds of female bacterial classifications are expanded grow cultivation, step is, carry out the routine sterilization to having a liking for the fiber bacterium culture medium, to have a liking for fiber starter kind inserts and the training of having a liking for the fiber bacterium culture medium is housed grows in the test tube, add 1g in every 100g substratum and have a liking for the fiber bacterial classification, having a liking for the every 1000g of fiber bacterium culture medium is by extractum carnis 5g, peptone 10g, Zulkovsky starch 2g, salt 6g, agar 19g and surplus are that water is formed, after having a liking for fiber starter kind implantation substratum, 26 ℃ of constant temperature, pH value is that 7.3 times trainings were grown 84 hours, breed into the test tube kind, insert training again and grow and carry out anaerobically fermenting in the jar, stand-by 26 ℃ of following anaerobically fermentings 200 hours; The cereuisiae fermentum bacterium culture medium is carried out the routine sterilization, saccharomyces cerevisiae after the sterilization adds in the microzyme culture medium, add cereuisiae fermentum bacterial classification 1g in every 100ml substratum, shake-flask culture is 84 hours under 26 ℃ of constant temperature, numerous saccharomyces cerevisiae test tube kind that expands into, said cereuisiae fermentum bacterium culture medium transfers to 1000ml by glucose 30g, yeast powder 20g with fermented bean drink and forms, the saccharomyces cerevisiae test tube kind that expands numerous one-tenth insert again training grow jar in 26 ℃ of following anaerobically fermentings 200 hours, become to expand the saccharomyces cerevisiae of growing, standby; The streptomyces rimosus substratum is carried out 120 ℃ of high pressure moist heat sterilizations, the training that the female kind of streptomyces rimosus is inserted the streptomyces rimosus substratum that the bacterium of going out is housed is grown in the jar, add 1g streptomyces rimosus kind in every 100ml streptomyces rimosus substratum, pH value is 7.3, constant temperature is trained down for 26 ℃ and was grown 84 hours, breed into the test tube kind, said streptomyces rimosus substratum is that every 1000ml is by Zulkovsky starch 20g, saltpetre 1g, salt 0.5g, K
2HPO
43H
2O 0.5g, MgSO
47H
2O 0.5g, FeSO
47H
2O 0.01g, agar 20g and surplus are that water is made, and pH value is 7.3, during preparation, elder generation, pours in the water that boils Zulkovsky starch furnishing pasty state with cold water again, heats on fire, add other compositions while stirring, fully after the dissolving evenly, supply moisture to 1000ml, the test tube kind streptomyces rimosus of breeding is inserted training again and is grown in the jar, under 18-30 ℃ temperature, carried out anaerobically fermenting 200 hours, the streptomyces rimosus that expansion is grown is grown in training, and is standby; Three kinds are expanded have a liking for fiber bacterium, saccharomyces cerevisiae and the streptomyces rimosus grow and are mixed with 1: 1: 1 ratio of weight and form mixed bacterium together behind anaerobically fermenting, insert in the culture tank again, adding wheat bran or rice bran expands numerous, carrying out solid under 18-28 ℃ expanded numerous 240 hours, add mixed bacterium 1g in every 100g wheat bran or the rice bran, solid expands to be put into training again after numerous and grows jar, 25 ℃ of constant temperature bottom fermentations 200 hours, become bacterium powder, water content 3% behind the drying and dehydrating.
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| CN102113623B (en) * | 2011-03-25 | 2012-11-14 | 曹孟 | Method for producing biological particle grain-saving straw feed |
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| CN102690778B (en) * | 2012-06-06 | 2013-11-06 | 河北圣雪大成制药有限责任公司 | Method for preparing streptomyces rimosus spores |
| CN103343101B (en) * | 2013-06-21 | 2015-03-25 | 赤峰创诺医药科技有限公司 | Inclined surface culture medium for terramycin strains |
| CN104557314A (en) * | 2015-01-19 | 2015-04-29 | 曹明军 | Multipurpose plant foliar nutrient solution and preparation method thereof |
| CN106635831A (en) * | 2016-12-03 | 2017-05-10 | 青岛漾花湖农业科技有限公司 | Culture medium for glomus mosseae and culture method |
| CN106511476A (en) * | 2016-12-21 | 2017-03-22 | 南阳师范学院 | Blood pressure, lipid and glucose reducing oral liquid for elderly |
| CN108192836A (en) * | 2017-12-19 | 2018-06-22 | 浙江沃华环境科技有限公司 | A kind of complex microorganism bacterium solution and the decomposed method of poultry solid manure |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102113623B (en) * | 2011-03-25 | 2012-11-14 | 曹孟 | Method for producing biological particle grain-saving straw feed |
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