CN109997872A - A kind of microbial-bacterial fertilizer and its preparation method and application - Google Patents
A kind of microbial-bacterial fertilizer and its preparation method and application Download PDFInfo
- Publication number
- CN109997872A CN109997872A CN201910215071.6A CN201910215071A CN109997872A CN 109997872 A CN109997872 A CN 109997872A CN 201910215071 A CN201910215071 A CN 201910215071A CN 109997872 A CN109997872 A CN 109997872A
- Authority
- CN
- China
- Prior art keywords
- culture
- microbial
- burkholderia
- bacterial fertilizer
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of microbial-bacterial fertilizer and its preparation method and application, microbial-bacterial fertilizer is by bacillus subtilis and Burkholderia according to (1~3): (1~3) is compounded；The bacterial concentration of Bacillus subtillis and Burkholderia is 5x108cfu/ml.Microbial-bacterial fertilizer of the invention is good to persimmon anthracnose control efficiency, no chemical residues and is not likely to produce drug resistance, moreover it is possible to persimmon be promoted to increase production 20% or more.
Description
Technical field
The present invention relates to technical field of fertilizer production, more particularly to a kind of microbial-bacterial fertilizer and preparation method thereof
And application.
Background technique
Anthracnose is one of persimmon most serious disease, and especially in recent years, many farmers are made due to this disease
At the serious underproduction, or even have no result and can receive, by its harm can seriously fallen leaves, shedding, young sprout is withered or even whole strain is dead.Fruit
It catches an illness, mostly occurs in late June to early July, picking time can also be extended to；Just syringe needle size dark brown is generated to black in fruit face
Color fleck, rear expand is round or oval, slightly concave to fall into, and periphery is in yellowish-brown, and 5~25 microns of diameter；The dense grey in center
The granule point arranged to black wheel line shape when meeting rain or high humidity, overflows pink sticky matter matter；Scab often go deep into cortex hereinafter,
Black lump is formed in fruit, generally gives birth to 1~2 scab, more person's dozens ofs, normal early fallout in a disease fruit.Blade is caught an illness, more
Betide petiole and vein, first yellowish-brown, after become dark brown to black, strip or irregular shape.Young sprout is caught an illness, and is mostly occurred
In late May to early June, black circle fleck, rear dimmed brown initially are generated in surface, scab expands in long oval
Shape, middle part is slightly concave to be fallen into and shows brown lobe, generates black small grain point, i.e. germ aceravlus thereon.Black when wet weather
Gush out red sticky matter, i.e. spore ball on scab.Scab is 10~20 millimeters long, and its underpart xylem is rotten, and the sick tip easily fractures；
When scab is big on branch, the above branch of scab is easily withered.
Currently, the prevention and treatment for persimmon anthracnose, on the basis reinforced cultivation management measure, improve plant disease resistance
On, still based on chemical agent, common fungicide have Prochloraz, difenoconazole, Tebuconazole, mancozeb, thiophanate-methyl,
Bravo, carbendazim, iprodione, anilazine, paclobutrazol etc..However, due to continuous, the unreasonable use of chemical pesticide, not only
Pesticide residue and environmental pollution are caused, also pathogen is made to produce drug resistance, reduces control efficiency.
Therefore it provides a kind of pair of persimmon anthracnose control efficiency is good, no chemical residues, the microorganism for being not likely to produce drug resistance
The problem of bacterial manure and preparation method thereof is those skilled in the art's urgent need to resolve.
Summary of the invention
In view of this, the present invention provides a kind of microbial-bacterial fertilizer and its preparation method and application, the microbial-bacterial fertilizer pair
Persimmon anthracnose control efficiency is good, no chemical residues and is not likely to produce drug resistance.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of microbial-bacterial fertilizer, by bacillus subtilis and Burkholderia according to (1~3): (1~3) is multiple
With forming；The bacterial concentration of the Bacillus subtillis and Burkholderia is 5x108cfu/ml。
Further, a kind of preparation method of microbial-bacterial fertilizer, the specific steps are as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred respectively in Fresh
The test tube slant that is put into of solid medium on, in 30~37 DEG C of 15~20h of culture, the switching of this slant strains is taken to train containing liquid
The shaking flask culture for supporting base, in 30~37 DEG C of 24~36h of culture；
B, seed tank culture: being added fluid nutrient medium in seeding tank, and liquid amount is 30%~60%, adjusts pH to 6.6
~7.8,121 DEG C of 20~30min of sterilizing open cooling water temperature, and logical filtrated air control tank pressure is 0.04~0.06MPa, when
When temperature is down to 30~37 DEG C, the thallus in shaking flask is cleaned with 100~200ml sterile saline, is seeded in seeding tank
Fluid nutrient medium in, keep 30~37 DEG C, tank press 0.04~0.06MPa, carry out deep ventilation stir culture 16~for 24 hours；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 5%~10% inoculum concentration3The liquid of fermentor
In culture medium, 30~37 DEG C, tank 0.04~0.06MPa of pressure are kept, 24~30h of deep ventilation stir culture is carried out, makes withered grass bud
The bacterial concentration of born of the same parents bacillus and Burkholderia reaches 5x108cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
By volume (1~3) by the bacillus subtilis of step (1) preparation and Burkholderia bacterium solution: (1
~3) it is uniformly mixed to get microbial-bacterial fertilizer.
Further, the formula of the solid medium are as follows: peptone 10g, beef extract 7g, sodium chloride 7g, distilled water constant volume
To 1000ml, pH 7.2~7.4 is adjusted, 18~20g agar is added.
Further, the formula of the fluid nutrient medium are as follows: peptone 20g, yeast extract 5g, dipotassium hydrogen phosphate 0.2g, phosphoric acid
Potassium dihydrogen 2g, magnesium sulfate 0.1g, glucose 8g, calcium carbonate 10g, distilled water are settled to 1000ml.
Bacillus subtilis and Burkholderia bacterium solution are pressed volume by the application method of the microbial-bacterial fertilizer
Than (1~3): (1~3) is uniformly mixed, and is diluted with water 100~500 times, is sprayed on persimmon.
Application of the microbial-bacterial fertilizer in prevention and treatment persimmon anthracnose.
It can be seen via above technical scheme that compared with prior art, the present disclosure provides a kind of microbial-bacterial fertilizers
And its preparation method and application, the microbial-bacterial fertilizer is by bacillus subtilis and Burkholderia according to (1~3):
(1~3) compounds, and, no chemical residues good to persimmon anthracnose control efficiency and is not likely to produce drug resistance, moreover it is possible to promote persimmon
20% or more volume increase.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is common
Technical staff's every other embodiment obtained without making creative work belongs to the model that the present invention protects
It encloses.
Bacillus subtilis (Bacillus subtilis) number is ATCC 21951；Burkholderia
(Burkholderia cepacia) number is ATCC 25416；It is that purchase obtains.
Embodiment 1
A kind of preparation method of microbial-bacterial fertilizer, the specific steps are as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred respectively in Fresh
The test tube slant that is put into of solid medium on, in 30 DEG C of culture 20h, this slant strains is taken to transfer shaking containing fluid nutrient medium
Bottle culture, in 30 DEG C of culture 36h；
B, seed tank culture: being added fluid nutrient medium in seeding tank, liquid amount 30%, adjusts pH to 6.6, and 121 DEG C
Sterilize 20min, cooling water temperature is opened, and logical filtrated air control tank pressure is 0.04MPa, when temperature is down to 30 DEG C, by shaking flask
In thallus cleaned with 100ml sterile saline, be seeded in the fluid nutrient medium in seeding tank, keep 30 DEG C, tank pressure
0.04MPa carries out deep ventilation stir culture for 24 hours；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 10% inoculum concentration3The Liquid Culture of fermentor
In base, 30 DEG C, tank pressure 0.04MPa are kept, deep ventilation stir culture 30h is carried out, makes Bacillus subtillis and onion Bai Kehuo
You reach 5x10 by the bacterial concentration of moral Salmonella8cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
The bacillus subtilis of step (1) preparation is mixed at 1: 3 by volume with Burkholderia bacterium solution
It is even to get microbial-bacterial fertilizer.
Embodiment 2
A kind of preparation method of microbial-bacterial fertilizer, the specific steps are as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred respectively in Fresh
The test tube slant that is put into of solid medium on, in 37 DEG C of culture 15h, this slant strains is taken to transfer shaking containing fluid nutrient medium
Bottle culture, for 24 hours in 37 DEG C of cultures；
B, seed tank culture: being added fluid nutrient medium in seeding tank, liquid amount 40%, adjusts pH to 7.8, and 121 DEG C
Sterilize 30min, cooling water temperature is opened, and logical filtrated air control tank pressure is 0.06MPa, when temperature is down to 37 DEG C, by shaking flask
In thallus cleaned with 200ml sterile saline, be seeded in the fluid nutrient medium in seeding tank, keep 37 DEG C, tank pressure
0.06MPa carries out deep ventilation stir culture 16h；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 5% inoculum concentration3The fluid nutrient medium of fermentor
In, 37 DEG C, tank pressure 0.06MPa are kept, deep ventilation stir culture is carried out for 24 hours, makes primary gram of Hall of Bacillus subtillis and onion
The bacterial concentration of moral Salmonella reaches 5x108cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
The bacillus subtilis of step (1) preparation is mixed at 1: 1 by volume with Burkholderia bacterium solution
It is even to get microbial-bacterial fertilizer.
Embodiment 3
A kind of preparation method of microbial-bacterial fertilizer, the specific steps are as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred respectively in Fresh
The test tube slant that is put into of solid medium on, in 35 DEG C of culture 18h, this slant strains is taken to transfer shaking containing fluid nutrient medium
Bottle culture, in 35 DEG C of culture 30h；
B, seed tank culture: being added fluid nutrient medium in seeding tank, liquid amount 50%, adjusts pH to 7.2, and 121 DEG C
Sterilize 25min, cooling water temperature is opened, and logical filtrated air control tank pressure is 0.05MPa, when temperature is down to 35 DEG C, by shaking flask
In thallus cleaned with 150ml sterile saline, be seeded in the fluid nutrient medium in seeding tank, keep 35 DEG C, tank pressure
0.05MPa carries out deep ventilation stir culture 20h；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 8% inoculum concentration3The fluid nutrient medium of fermentor
In, 35 DEG C, tank pressure 0.05MPa are kept, deep ventilation stir culture 27h is carried out, makes primary gram of Hall of Bacillus subtillis and onion
The bacterial concentration of moral Salmonella reaches 5x108cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
The bacillus subtilis of step (1) preparation is mixed at 3: 1 by volume with Burkholderia bacterium solution
It is even to get microbial-bacterial fertilizer.
Embodiment 4
A kind of preparation method of microbial-bacterial fertilizer, the specific steps are as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred respectively in Fresh
The test tube slant that is put into of solid medium on, in 30 DEG C of culture 20h, this slant strains is taken to transfer shaking containing fluid nutrient medium
Bottle culture, in 30 DEG C of culture 36h；
B, seed tank culture: being added fluid nutrient medium in seeding tank, liquid amount 30%, adjusts pH to 6.6, and 121 DEG C
Sterilize 20min, cooling water temperature is opened, and logical filtrated air control tank pressure is 0.04MPa, when temperature is down to 30 DEG C, by shaking flask
In thallus cleaned with 100ml sterile saline, be seeded in the fluid nutrient medium in seeding tank, keep 30 DEG C, tank pressure
0.04MPa carries out deep ventilation stir culture for 24 hours；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 10% inoculum concentration3The Liquid Culture of fermentor
In base, 30 DEG C, tank pressure 0.04MPa are kept, deep ventilation stir culture 30h is carried out, makes Bacillus subtillis and onion Bai Kehuo
You reach 5x10 by the bacterial concentration of moral Salmonella8cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
The bacillus subtilis of step (1) preparation is mixed at 1: 2 by volume with Burkholderia bacterium solution
It is even to get microbial-bacterial fertilizer.
Embodiment 5
A kind of preparation method of microbial-bacterial fertilizer, the specific steps are as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred respectively in Fresh
The test tube slant that is put into of solid medium on, in 30 DEG C of culture 20h, this slant strains is taken to transfer shaking containing fluid nutrient medium
Bottle culture, in 30 DEG C of culture 36h；
B, seed tank culture: being added fluid nutrient medium in seeding tank, liquid amount 30%, adjusts pH to 6.6, and 121 DEG C
Sterilize 20min, cooling water temperature is opened, and logical filtrated air control tank pressure is 0.04MPa, when temperature is down to 30 DEG C, by shaking flask
In thallus cleaned with 100ml sterile saline, be seeded in the fluid nutrient medium in seeding tank, keep 30 DEG C, tank pressure
0.04MPa carries out deep ventilation stir culture for 24 hours；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 10% inoculum concentration3The Liquid Culture of fermentor
In base, 30 DEG C, tank pressure 0.04MPa are kept, deep ventilation stir culture 30h is carried out, makes Bacillus subtillis and onion Bai Kehuo
You reach 5x10 by the bacterial concentration of moral Salmonella8cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
The bacillus subtilis of step (1) preparation is mixed at 2: 1 by volume with Burkholderia bacterium solution
It is even to get microbial-bacterial fertilizer.
Comparative example 1
A kind of microbial-bacterial fertilizer, contains only bacillus subtilis, and the bacterial concentration of Bacillus subtillis is 5x108cfu/
ml。
Comparative example 2
A kind of microbial-bacterial fertilizer contains only Burkholderia, the bacterial concentration of Burkholderia
For 5x108cfu/ml。
(1) respectively by embodiment 1-5, the microbial-bacterial fertilizer that comparative example 1-2 is obtained dilutes 100 times, for spraying.
Persimmon anthracnose controlling experiment uses field trial, and selection growing way is close, the almost the same persimmon of anthracnose illness；
Each 50 plants of processing cell, (microbial-bacterial fertilizer of embodiment 1-5, comparative example 1-2 dilute 100 times for each processing；Reference examples: clear
Water) each 3 repetitions, it is arranged using random district's groups, processing minizone stays a line to make protection row, with knapsack electric sprayer upper
It is sprayed after noon dew is dry, is sprayed on the trunk, branch and leaf of persimmon；In the first tenday period of a month in May, start to spray, sprays one every 15d
It is secondary, it sprays altogether 4 times；3 duplicate average diseased plant numbers after spraying every time are recorded before spray next time, after spraying for the last time
15d records the anthracnose recurrence of 3 duplicate average diseased plant numbers and second year and third year, and calculating and reference examples
It compares, each rate of growth for handling First Year, the results are shown in Table 1.
Table 1
As shown in Table 1, using microbial-bacterial fertilizer of the invention, persimmon anthracnose can be efficiently prevented and treated, control efficiency is obvious
Better than exclusive use bacillus subtilis or Burkholderia；And it is not recurred substantially using after 3 years.
(2) respectively by embodiment 1-5, the microbial-bacterial fertilizer that comparative example 1-2 is obtained dilutes 300 times, for spraying.
Persimmon anthracnose controlling experiment uses field trial, and selection growing way is close, the almost the same persimmon of anthracnose illness；
Each 50 plants of processing cell, (microbial-bacterial fertilizer of embodiment 1-5, comparative example 1-2 dilute 300 times for each processing；Reference examples: clear
Water) each 3 repetitions, it is arranged using random district's groups, processing minizone stays a line to make protection row, with knapsack electric sprayer upper
It is sprayed after noon dew is dry, is sprayed on the trunk, branch and leaf of persimmon；In the first tenday period of a month in May, start to spray, sprays one every 15d
It is secondary, it sprays altogether 4 times；3 duplicate average diseased plant numbers after spraying every time are recorded before spray next time, after spraying for the last time
15d records the anthracnose recurrence of 3 duplicate average diseased plant numbers and second year and third year, and calculating and reference examples
It compares, each rate of growth for handling First Year, the results are shown in Table 2.
Table 2
As shown in Table 2, using microbial-bacterial fertilizer of the invention, persimmon anthracnose can be efficiently prevented and treated, control efficiency is obvious
Better than exclusive use bacillus subtilis or Burkholderia；And it is not recurred substantially using after 3 years.
(3) respectively by embodiment 1-5, the microbial-bacterial fertilizer that comparative example 1-2 is obtained dilutes 500 times, for spraying.
Persimmon anthracnose controlling experiment uses field trial, and selection growing way is close, the almost the same persimmon of anthracnose illness；
Each 50 plants of processing cell, (microbial-bacterial fertilizer of embodiment 1-5, comparative example 1-2 dilute 500 times for each processing；Reference examples: clear
Water) each 3 repetitions, it is arranged using random district's groups, processing minizone stays a line to make protection row, with knapsack electric sprayer upper
It is sprayed after noon dew is dry, is sprayed on the trunk, branch and leaf of persimmon；In the first tenday period of a month in May, start to spray, sprays one every 15d
It is secondary, it sprays altogether 4 times；3 duplicate average diseased plant numbers after spraying every time are recorded before spray next time, after spraying for the last time
15d records the anthracnose recurrence of 3 duplicate average diseased plant numbers and second year and third year, and calculating and reference examples
It compares, each rate of growth for handling First Year, the results are shown in Table 3.
Table 3
As shown in Table 3, using microbial-bacterial fertilizer of the invention, persimmon anthracnose can be efficiently prevented and treated, control efficiency is obvious
Better than exclusive use bacillus subtilis or Burkholderia；And it is not recurred substantially using after 3 years.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be readily apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest scope of cause.
Claims (5)
1. a kind of microbial-bacterial fertilizer, which is characterized in that by bacillus subtilis and Burkholderia according to (1~3):
(1~3) compounds；The bacterial concentration of the Bacillus subtillis and Burkholderia is 5x108cfu/ml。
2. a kind of preparation method of microbial-bacterial fertilizer according to claim 1, which is characterized in that specific step is as follows:
(1) bacillus subtilis and Burkholderia bacterium solution are prepared:
A, Spawn incubation: the bacillus subtilis of preservation and Burkholderia are transferred consolidating in Fresh respectively
On the test tube slant that body culture medium is put into, in 30~37 DEG C of 15~20h of culture, the switching of this slant strains is taken to contain fluid nutrient medium
Shaking flask culture, in 30~37 DEG C of 24~36h of culture；
B, seed tank culture: being added fluid nutrient medium in seeding tank, and liquid amount is 30%~60%, and adjusting pH to 6.6~
7.8,121 DEG C of 20~30min of sterilizing open cooling water temperature, and logical filtrated air control tank pressure is 0.04~0.06MPa, works as temperature
When degree is down to 30~37 DEG C, the thallus in shaking flask is cleaned with 100~200ml sterile saline, is seeded in seeding tank
In fluid nutrient medium, 30~37 DEG C of holding, tank 0.04~0.06MPa of pressure, progress deep ventilation stir culture 16~for 24 hours；
C, the culture solution in seeding tank fermentation tank culture: is transferred to 1m with 5%~10% inoculum concentration3The Liquid Culture of fermentor
In base, 30~37 DEG C, tank 0.04~0.06MPa of pressure are kept, 24~30h of deep ventilation stir culture is carried out, makes Ko subtilis bar
The bacterial concentration of bacterium and Burkholderia reaches 5x108cfu/ml；
(2) bacillus subtilis mixes with Burkholderia bacterium solution:
By volume (1~3) by the bacillus subtilis of step (1) preparation and Burkholderia bacterium solution: (1~3)
It is uniformly mixed to get microbial-bacterial fertilizer.
3. a kind of preparation method of microbial-bacterial fertilizer according to claim 2, which is characterized in that the solid medium
Formula are as follows: peptone 10g, beef extract 7g, sodium chloride 7g, distilled water are settled to 1000ml, adjust pH 7.2~7.4, are added 18
~20g agar.
4. a kind of preparation method of microbial-bacterial fertilizer according to claim 2, which is characterized in that the fluid nutrient medium
Formula are as follows: peptone 20g, yeast extract 5g, dipotassium hydrogen phosphate 0.2g, potassium dihydrogen phosphate 2g, magnesium sulfate 0.1g, glucose 8g, carbon
Sour calcium 10g, distilled water are settled to 1000ml.
5. a kind of microbial-bacterial fertilizer according to claim 1, the application in prevention and treatment persimmon anthracnose.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910215071.6A CN109997872A (en) | 2019-03-20 | 2019-03-20 | A kind of microbial-bacterial fertilizer and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910215071.6A CN109997872A (en) | 2019-03-20 | 2019-03-20 | A kind of microbial-bacterial fertilizer and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109997872A true CN109997872A (en) | 2019-07-12 |
Family
ID=67167539
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910215071.6A Pending CN109997872A (en) | 2019-03-20 | 2019-03-20 | A kind of microbial-bacterial fertilizer and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109997872A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870608A (en) * | 2010-06-21 | 2010-10-27 | 山东宝源生物有限公司 | Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof |
| CN105802874A (en) * | 2015-11-03 | 2016-07-27 | 山东鲁虹肥料研究院 | Mixed bacterium for efficiently degrading atrazine and fermental culture method |
| CN106883998A (en) * | 2017-03-23 | 2017-06-23 | 河南工业大学 | A kind of preparation method of microorganism multifunctional fertilizer is constituted with fermentation medium |
| CN107446864A (en) * | 2017-09-02 | 2017-12-08 | 哈尔滨商业大学 | A kind of compound microorganism ferments microbial inoculum, preparation method and application |
| CN107827485A (en) * | 2017-11-13 | 2018-03-23 | 淮阴师范学院 | A kind of bio-bacterial manure, preparation method and its application in soil phosphorus decomposing |
| CN107926983A (en) * | 2017-11-27 | 2018-04-20 | 大工（青岛）新能源材料技术研究院有限公司 | Probiotics of biological and ecological methods to prevent plant disease, pests, and erosion anthracnose of tomato and preparation method thereof |
| CN109452312A (en) * | 2018-12-12 | 2019-03-12 | 华南农业大学 | Raw Burkholderia and its biological and ecological methods to prevent plant disease, pests, and erosion application in a kind of sugarcane |
-
2019
- 2019-03-20 CN CN201910215071.6A patent/CN109997872A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870608A (en) * | 2010-06-21 | 2010-10-27 | 山东宝源生物有限公司 | Continuous cropping resisting biological organic and inorganic compound fertilizer and production method thereof |
| CN105802874A (en) * | 2015-11-03 | 2016-07-27 | 山东鲁虹肥料研究院 | Mixed bacterium for efficiently degrading atrazine and fermental culture method |
| CN106883998A (en) * | 2017-03-23 | 2017-06-23 | 河南工业大学 | A kind of preparation method of microorganism multifunctional fertilizer is constituted with fermentation medium |
| CN107446864A (en) * | 2017-09-02 | 2017-12-08 | 哈尔滨商业大学 | A kind of compound microorganism ferments microbial inoculum, preparation method and application |
| CN107827485A (en) * | 2017-11-13 | 2018-03-23 | 淮阴师范学院 | A kind of bio-bacterial manure, preparation method and its application in soil phosphorus decomposing |
| CN107926983A (en) * | 2017-11-27 | 2018-04-20 | 大工（青岛）新能源材料技术研究院有限公司 | Probiotics of biological and ecological methods to prevent plant disease, pests, and erosion anthracnose of tomato and preparation method thereof |
| CN109452312A (en) * | 2018-12-12 | 2019-03-12 | 华南农业大学 | Raw Burkholderia and its biological and ecological methods to prevent plant disease, pests, and erosion application in a kind of sugarcane |
Non-Patent Citations (4)
| Title |
|---|
| VEERUBOMMU SHANMUGAM,ET AL.: "Genetic diversity of Fusarium spp. inciting rhizome rot of ginger and its management by PGPR consortium in the western Himalayas", 《BIOLOGICAL CONTROL》 * |
| 余贤美等: "枯草芽孢杆菌Bs-15对柿树炭疽病的离体防治效果", 《山东农业科学》 * |
| 林超等: "土壤嗜铁细菌C19的筛选、分子鉴定及其对炭疽菌和镰刀菌的拮抗作用", 《热带作物学报》 * |
| 邱强: "《中国果树病虫原色图鉴》", 31 January 2004, 河南科学技术出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107254427B (en) | Bacillus belgii strain JN5 and application thereof | |
| CN109182199B (en) | Pseudomonas brassicae with plant growth promoting effect | |
| CN100451105C (en) | Bacillus laterosporus and soil inoculation agent prepared from the strain | |
| CN106399159B (en) | A kind of bacillus firmus microbial inoculum and the preparation method and application thereof | |
| CN100445370C (en) | Bacillus subtilis, bacterium agent and application thereof | |
| CN105557757B (en) | A kind of preparation method of Methylotrophic bacillus wettable powder | |
| CN114437982B (en) | Bacillus amyloliquefaciens for improving soil fertilizer efficiency and application thereof | |
| CN105439723A (en) | Bacillus amyloliquefaciens insecticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN105439725A (en) | Paenibacillus polymyxa pesticide-fertilizer for farm onsite fermentation and applications thereof | |
| CN113862189A (en) | Corn growth promoting, probiotic and biocontrol bacterial fertilizer integrated preparation and preparation method thereof | |
| CN107699523A (en) | One plant of can degrade insecticide Buprofezin and the bacterium of Biphenthrin and its microbial inoculum of production | |
| CN111670769B (en) | Method for improving stress resistance of rice | |
| CN110317802A (en) | A kind of microbial bacterial agent and preparation method thereof for preventing and treating ring rot of apple | |
| CN104671854A (en) | Preparation method of compound photosynthetic bacterial fertilizer | |
| CN106701619B (en) | high-density fermentation method of bacillus amyloliquefaciens and preparation method of microbial inoculum thereof | |
| CN110468080A (en) | Microbial bacterial agent of growth-promoting rice and drop cadmium and preparation method thereof and application method | |
| CN110122485A (en) | A kind of trichoderma, Trichoderma and preparation method thereof | |
| CN111466266B (en) | Method for promoting rice growth and iron element absorption in rice iron-deficient environment | |
| CN112481161A (en) | Microbial agent for promoting wheat growth and wheat breeding method thereof | |
| CN105145632B (en) | It is a kind of with the compound bacteria enzyme system of multiple effect and its application | |
| CN114350552B (en) | Bacillus pumilus and application thereof in control of medlar diseases | |
| CN110982740A (en) | Paenibacillus mucilaginosus and application thereof | |
| CN110511875A (en) | A kind of compounding biocontrol agent and the preparation method and application thereof | |
| CN109997872A (en) | A kind of microbial-bacterial fertilizer and its preparation method and application | |
| CN109135762A (en) | A kind of microbial-bacterial fertilizer and preparation method thereof for promoting tangerine orange to grow |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190712 |