CN107699523A - One plant of can degrade insecticide Buprofezin and the bacterium of Biphenthrin and its microbial inoculum of production - Google Patents
One plant of can degrade insecticide Buprofezin and the bacterium of Biphenthrin and its microbial inoculum of production Download PDFInfo
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Abstract
The invention belongs to the biological prosthetic field of environmental pollution, degraded insecticide Buprofezin and the bacterium of Biphenthrin and its microbial inoculum of production can be stablized by disclosing one plant, September in 2017 is preserved in China typical culture collection center on the 25th, and culture presevation number is CCTCC NO.M 2017537.The bacterial strain is G+ bacteria strain D 6, is identified as Rhod (Rhodococcus sp.).Described degradation bacteria strains D 6 of the present invention can be used for the degraded of Buprofezin and bifenthrin insecticide, the residual quantity that can make the Buprofezin in soil in a short time using this microbial inoculum reduces by more than 95%, make the residual quantity of the Biphenthrin in soil reduce by more than 70% within a certain period of time, its character of degrading is more stable compared to the degradation bacteria strains Rhodococcus sp.YL 1 reported, available for the Buprofezin and Biphenthrin residual contamination removed in environment.
Description
Technical field
Biological high-tech field of the present invention, disclose one plant can stablize degraded insecticide Buprofezin and Biphenthrin it is thin
Bacterium and its microbial inoculum of production.
Background technology
Chemical pesticide the disease of crop, worm, crop smothering the comprehensive regulation in play an important role, Modern Agriculture is greatly facilitated
The development of industry, but serious environmental problem is also brought simultaneously.First, agricultural product because residues of pesticides it is exceeded, quality reduce, go out
Mouth is limited；Secondly, residues of pesticides can be enriched with by food chain, threaten the life and health of the mankind；In addition, residues of pesticides harm is non-
Target organismses, change ecosystem structure indirectly, destroy its function.
Buprofezin is a kind of insecticide of the triazines of high-efficiency broad spectrum, to pest controling effects such as plant hopper, leafhopper and aleyrodids
Significantly.Widely applied from the nineties in last century, Buprofezin residual it is exceeded the problem of appear in the newspapers repeatly.Buprofezin is aerobic
The half-life period in field is 50 to 70 days, and its half-life period is 36-104 days in the rice field of water logging.Buprofezin residual pair in environment
Aquatile and some non-target beneficial insect harm are serious, and easy direct oral cavity, skin surface and respiratory tract are absorbed by the body, to the mankind
Health causes potential threat.Biphenthrin is a kind of insecticide of efficient pyrethroid, is widely used in agricultural production
And urban environment.Biphenthrin is not only endangered seriously aquatile, and to human health, there is also potential hazard.2009,
Biphenthrin is included in by the World Health Organization (WHO) in the list of two level (poisoning) industrial goods.
Because Buprofezin and Biphenthrin all have preferable prevention effect to the small greenery mite of tea in tea tree, plus peasant household
Use lack of standardization to agricultural chemicals, cause combined pollution of two kinds of insecticides in tea garden soil.Pesticide residues warp in soil
Rain drop erosion enters water body, causes the pollution in environment, and potential threat is caused to human health.Based on microbial degradation
Bioremediation technology have safely, effectively, inexpensively, non-secondary pollution the characteristics of, it has a extensive future.It can degrade simultaneously
The microorganism of Buprofezin and Biphenthrin is more advantageous in the application.Buprofezin is similar with the structure of Biphenthrin, can be simultaneously
Degrade the only Rhodococcus sp.YL-1 of both insecticides, but its degrading genes is easy to be lost, Character instability of degrading,
It is unfavorable for carrying out large-scale industrial production, governs and Buprofezin in environment and Biphenthrin residual are repaiied using the bacterium
It is multiple.Therefore, it is necessary to continue that screening can degrade both agricultural chemicals simultaneously and the stable bacterial strain of character of degrading realizes the big rule of microbial inoculum
Mould industrialized production, realize to the reparation in environment to both residues of pesticides.
The content of the invention
It is an object of the invention to for problem above, develop a kind of stable residues of pesticides of character for technical problem
Degradation bacterial agent, the residual quantity that can make Buprofezin using this microbial inoculum reduce by more than 95%, make the residual quantity of Biphenthrin reduce
More than 70%.
It is the main contents of this programme below technical scheme：
The present invention provides a kind of degradation bacteria for eliminating Buprofezin and Biphenthrin, and its bacterial strain is one plant of Gram's staining reaction
(September in 2017 is deposited at Type Tissue Collection on the 25th to positive bacteria D-6, and culture presevation numbering is CCTCC NO.M
2017537), it is identified as Rhod (Rhodococcus sp.).Its main biological characteristics is Gram's staining sun
Property, no gemma, cellular morphology is in rod-short.Colonial morphology is that lightpink is opaque, the smooth of the edge, protuberance, quality are sticky and wet
Profit.It can not move, it is aerobic.Under the conditions of laboratory shake flask, to 50mgL in 48h-1Buprofezin degradation rate be up to 95% with
On, to 50mgL in 72h-1Biphenthrin degradation rate be more than 70%.Bacterial strain D-6 degraded character is than the thiophene reported
Piperazine ketone, Biphenthrin degradation bacteria strains Rhodococcus sp.YL-1 are more stable, bacterial strain YL-1 continuous passages 3 in LB culture mediums
After secondary, its character Loss Rate of degrading is 1%, and in LB culture mediums after continuous passage 10 times, its character Loss Rate of degrading is for it
15%, and bacterial strain D-6 has no the bacterium colony that character is lost after equal passage number, therefore carry out fermentation industry using bacterial strain D-6
Production has more advantage.
Using Buprofezin, Biphenthrin degradation bacteria strains D-6 production microbial inoculum technique be：Inclined-plane kind --- shake-flask seed
Liquid --- seeding tank --- product (packaging microbial inoculum is liquid bacterial agent or solid absorption microbial inoculum).
The present invention detailed implementation steps be：
1st, the test tube slant kind of Buprofezin, Biphenthrin residue degrading bacterial strain D-6 is inoculated in shaking containing LB culture mediums
In bottle, concussion and cultivate to logarithmic phase growth period；
The 2nd, above-mentioned cultured strain is seeded to 500L seeding tank according to 10% inoculum concentration, cultivated to logarithmic growth
Phase.The culture medium prescription of seeding tank is：Glucose 0.8%, (NH4)2SO41%, K2HPO40.2%, MgSO40.05%,
NaCl 0.01%, CaCO30.3%, yeast extract 0.02%, pH value is 7.2~7.5.
3rd, by seed liquor with 10% inoculum concentration access production tank culture, the culture medium for producing tank is identical with seeding tank.
In the production process of seeding tank and production tank, the throughput of filtrated air is volume ratio 1:0.6-1.2, stirring speed
Spend for 180-240 revs/min, cultivation temperature is 30 DEG C, and whole technological process incubation time is 96-108h.After the completion of fermentation, bacterium
Body quantity is up to 1,000,000,000/mL, and nutrient solution goes out tank and is directly distributed into liquid dosage form with plastic barrel or Packaging Bottle after the completion of fermentation
Or solid fungicide formulation is distributed into packaging bag using adsorption by peat.
Beneficial effect
The present invention provides a kind of bacterial strain D-6 of degraded Buprofezin residual that can be efficient, quick and stable.It is right in 48h
50mg·L-1Buprofezin degradation rate be up to more than 95%, to 50mgL in 72h-1Biphenthrin degradation rate be up to 70%
More than, and the bacterial strain Rhodococcus qingshengii YL-1 that its character of degrading has been reported are stable, therefore with extensive
Application potential and value.
There is the advantages of production and application cost is low, easy to use, removal effect is good using what the microbial inoculum produced, be adapted to complete
State's grain oil ＆ vegetable production export base or the place for having green brand mark promote the use of a large area.The present invention gives birth to for protection
State environment, people's health is protected, improving the added value of agricultural product has important meaning.The present invention successfully solves agricultural
The problem of Pesticide Residues are exceeded is produced, efficient snap action of the chemical pesticide in insect pest of the plant preventing and treating had both been played, and again might be used
Produce non-toxic and non-pollution green agricultural product.
Biomaterial preservation information
D-6, Classification And Nomenclature are Rhodococcus sp D-6 (Rhodococcus sp.D-6), and depositary institution is Chinese Typical Representative culture
Collection, the deposit number of bacterial strain are CCTCC NO.M 2017537, and preservation date is September in 2017 25, preservation address
For Wuhan, China Wuhan University.
Brief description of the drawings
Fig. 1 bacterial strains D-6 colonial morphology photo
The growth degradation curve of Fig. 2 bacterial strain D-6 parathiazine ketone
The HPLC-MS collection of illustrative plates of Fig. 3 bacterial strains D-6 degraded Buprofezins (A) and Biphenthrin (B) generation product
Influence of Fig. 4 temperature to bacterial strain D-6 degraded Buprofezins
Influences of Fig. 5 pH to bacterial strain D-6 degraded Buprofezins
Influence of the initial concentration of Fig. 6 Buprofezins to bacterial strain D-6 degraded Buprofezins
Embodiment
The separation and identification of the bacterial strain of embodiment 1
The present invention provides the microbial inoculum of a kind of character stabilization and the bacterial strain and its production of efficient degradation Buprofezin and Biphenthrin
D-6, Jiangsu Province is isolated from for a long time in by Buprofezin contaminated soil.The screening technique of bacterial strain is：5g soil samples are taken to be added to
100mL's contains 50mgL-1In the shaking flask of the basal salt media (hereinafter referred to as BMM) of Buprofezin, 30 DEG C, 150rpm are placed in
Shaking table in cultivate, be transferred to after 5 days with 5% inoculum concentration in the shaking flask containing fresh BMM, be carried out continuously three times enrichment training
Support.By the pregnant solution dilution spread of last time passage on BMM solid mediums, picking is formed transparent after 30 DEG C of cultures 5 days
The single bacterium of circle falls within the LB test tubes equipped with 4mL and cultivated.After thalline length is dense, a part of thalline is used to preserve, another part
Thalline is used for the detection of degrading activity to obtain the degradation bacteria strains of Buprofezin.The detection method of Buprofezin degraded is that thalline is transferred
Into the shaking flask equipped with 20mL BMM culture mediums, after being placed in 30 DEG C, cultivating 5 days in 150rpm shaking table, with isometric dichloro
Methane extracts, and passes through UV spectrophotometer measuring degradation effect.The detection method of Biphenthrin degraded is to be forwarded to thalline
Contain 50mgL equipped with 20mL-1In the shaking flask of the basal salt media of Biphenthrin, 30 DEG C are placed in, train in 150rpm shaking table
After supporting 3 days, the color change of culture medium is observed, and is extracted with isometric ethyl acetate, degraded effect is detected by UHPLC-MS
Fruit.
Bacterial strain D-6 is identified to belong to Rhodococcus sp., and Chinese Typical Representative culture is deposited within 25th in September in 2017
Collection, the deposit number of bacterial strain is CCTCC NO.M 2017537.The morphological feature that bacterial strain D-6 grows on LB flat boards is
Lightpink is opaque, the smooth of the edge, protuberance, quality are sticky and moisten, compared to reported Buprofezin, Biphenthrin degradation bacteria
Strain YL-1, bacterial strain D-6 colony colour is shallower, and shape is larger, and quality relatively moistens (Figure 1A, 1B).Main biological property is leather
Blue Albert'stain Albert is positive (Fig. 1 C), it is impossible to produces gemma, it is impossible to the aerobic bacteria of motion；Its methyl red test, amylolytic enzyme examination
Test, oxidase test and nitrate reductase enzyme test are feminine gender；Its V.P. experiments, urease test and catalase examination
It is the positive to test.Bacterial strain D-6 16S rRNA gene orders are compared to analysis in database EzBilCloud, as a result show bacterium
The affiliations that belong to of strain D-6 and Rhodococcus are nearest, wherein with Rhodococcus qingshengii JCM 15477T's
Similitude is 100%.Analyzed according to the evo-devo of colonial morphology, physiological and biochemical property and 16S rRNA gene orders, just
Bacterial strain D-6 is accredited as Rhodococcus sp. by step.
The laboratory degradation experiment of embodiment 2
It is prepared by 2.1 seed liquors
The single bacterium colony that picking has activated on flat board, it is inoculated in LB culture mediums, is placed in 30 DEG C, is trained in 150rpm shaking table
Support to OD600≈1.0.Thalline (4 DEG C, 6000rpm centrifugations 5min) is collected by centrifugation, is resuspended after being washed twice with basal salt media
Thalline is to OD600≈ 2.0, this is seed liquor.
The degraded of 2.2 bacterial strain D-6 parathiazine ketone
Bacterial strain D-6 seed liquor is seeded in BMM with 2% inoculum concentration, 30 DEG C is placed in, is cultivated in 150rpm shaking table
48h.3mL nutrient solutions are taken every 8h, HPLC is detected and calculated the concentration and degradation rate of remaining Buprofezin in culture medium, simultaneously will
The nutrient solution dilution spread LB solid mediums of timing sampling, thalli growth amount is calculated, draw the growth of bacterial strain D-6 parathiazine ketone
Degradation curve (such as Fig. 2).Bacterial strain D-6 can be grown using Buprofezin as sole carbon source, by 50mgL in 48h-1Buprofezin drop
Solution more than 95%.Fig. 3 A are the HPLC-MS collection of illustrative plates of bacterial strain D-6 degraded thiazine ketogenesis products.
Influence of 2.3 temperature to bacterial strain D-6 degraded Buprofezins
Bacterial strain D-6 seed liquor is seeded in BMM with 2% inoculum concentration, rotating speed is respectively placed in and is uniformly arranged to
150rpm, temperature are respectively to be cultivated in 20 DEG C, 25 DEG C, 30 DEG C and 37 DEG C of shaking table (middle co-cultivation 48h).Taken every 8h
3mL nutrient solutions, HPLC are detected and are calculated the concentration of remaining Buprofezin and degraded in culture medium, and temperature drops to bacterial strain D-6
Solve the influence of Buprofezin.As shown in figure 4, the degradation rate highest of bacterial strain D-6 parathiazine ketone at 30 DEG C, the most suitable scope of degraded are
25-30 DEG C, low temperature (20 DEG C) or high temperature (37 DEG C) can all influence degradation efficiency, and influence of the high temperature (37 DEG C) to degradation efficiency compared with
To be notable.
Influences of the 2.4 initial pH to bacterial strain D-6 degraded Buprofezins
MSM pH is adjusted to 4.0,5.0,6.0,7.0,8.0,9.0,10.0 with HCl and NaOH respectively.To different pH's
Final concentration of 50mgL is added in basal salt media-1Buprofezin, and seed liquor is seeded to respectively with 2% inoculum concentration
In above-mentioned pH 4.0~10.0 culture medium, 30 DEG C are placed in, 48h is cultivated in 150rpm shaking table.3mL nutrient solutions are taken every 8h,
HPLC is detected and is calculated the concentration of remaining Buprofezin and degraded in culture medium, determines shadows of the pH to bacterial strain D-6 degraded Buprofezins
Ring.As shown in figure 5, the degradation efficiency highest of bacterial strain D-6 parathiazine ketone in pH 7.0；, can be to it when pH is 5.0 and 9.0
Degrading activity has a certain impact；When pH is 4.0, bacterial strain D-6 degradation efficiency has the reduction of conspicuousness, and this shows bacterial strain
The degradation efficiency of D-6 parathiazine ketone in alkaline environment is better than in sour environment.
Influence of the initial concentration of 2.5 Buprofezins to bacterial strain D-6 degraded Buprofezins
It is respectively 30mgL that seed liquor is seeded into Buprofezin final concentration respectively with 2% inoculum concentration-1, 50mgL-1,
100mg·L-1And 200mgL-1Basal salt media in, be placed in 30 DEG C, 48h cultivated in 150rpm shaking table.Taken every 8h
3mL nutrient solutions, HPLC are detected and are calculated the concentration of remaining Buprofezin and degraded in culture medium, determine the initial concentration of Buprofezin
Influence to bacterial strain D-6 degraded Buprofezins.As shown in fig. 6, when the initial concentration of Buprofezin is less than 50mgL-1When, bacterial strain D-6
The degradation efficiency of parathiazine ketone is attained by more than 95% within 48h；And when the initial concentration of Buprofezin is 100mgL-1
When, bacterial strain D-6 degradation efficiencies of parathiazine ketone in 48h drop to 50%；When the initial concentration of Buprofezin is 200mgL-1
When, its Buprofezin degradation efficiency only has 30% in 48h, illustrates the Buprofezin of high concentration and has to bacterial strain D-6 degrading activity
Inhibitory action.
Degradeds of the 2.6 bacterial strain D-6 to Biphenthrin
Bacterial strain D-6 seed liquor is seeded to containing 50mgL with 2% inoculum concentration-1The basic salt culture of Biphenthrin
In base, 30 DEG C are placed in, is cultivated in 150rpm shaking table.Nutrient solution, which can be observed, after culture 72h obvious color change, compares
In control group, treatment group becomes yellow.Fig. 3 B are the HPLC-MS collection of illustrative plates that bacterial strain D-6 degraded Biphenthrins generate product.Pass through
Detection to remaining bifenthrin concentration in nutrient solution, calculates degradation efficiency of the bacterial strain to Biphenthrin.Bacterial strain D-6 is in 72h
It is interior can by Biphenthrin degrade more than 70%.
The stability to degradation of embodiment 3 is tested
Due to bacterial strain Rhodococcus qingshengii YL-1 (the CCTCC AB 2017132) Buprofezin reported
Degraded Character instability, it is therefore necessary to bacterial strain D-6 degrade character stability assess.Picking bacterial strain D-6 respectively
With the single bacterium colony of bacterial strain YL-1 formation transparent circles of fresh activation on BMM flat boards, the examination equipped with 4mL LB culture mediums is inoculated in
Guan Zhong, 30 DEG C are placed in, is cultivated in 150rpm shaking table to OD600≈1.0.This bacterium solution is forwarded to another with 1% inoculum concentration
In test tube equipped with 4mL LB culture mediums, with identical condition of culture culture to OD600≈ 1.0, this is the 1st passage.By the 1st
The bacterium solution dilution suitable multiple (10 of secondary passage-4~10-6) BMM flat boards are coated with afterwards, after bacterium colony is grown, statistics can not be formed
The clump count of bright circle accounts for the percentage of whole clump counts.10 passages are carried out successively, and can not be formed in each passage of statistics
The percentage of bright circle bacterium colony.Statistical result shows, bacterial strain YL-1 after continuous passage 3 times, is coated with LB after the dilution of its bacterium solution
BMM flat boards on, you can it was observed that the bacterium colony of transparent circle can not be formed, the bacterium colony accounts for the 1% of whole bacterium colonies, and the ratio with
The increase of passage number and increase, after continuous passage 10 times, bacterial strain YL-1 degraded character Loss Rate is up to 15%；Phase
Instead, bacterial strain D-6 is in LB after continuous passage 3 times, is not observed on the BMM flat boards being coated with after the dilution of its bacterium solution and is unable to shape
Into the bacterium colony of transparent circle, thus continue to pass on bacterial strain D-6.After the 10th passage, it is coated with after the dilution of its bacterium solution
The bacterium colony of transparent circle can not be formed by being still not observed on BMM flat boards.The bacterium colony of this two plants of bacterium energy on BMM solid mediums
Thus can no formation transparent circle and the Buprofezin that degrade illustrate bacterial strain D-6 Buprofezin degraded there is one-to-one relation
Character is more stable.
The soil degrading of embodiment 4 is tested
Zijin at the foot of the hill rural area soil is picked up from for examination soil sample, crosses 2mm sieves, it is stand-by.It is female with the methanol of Buprofezin or Biphenthrin
Liquid soaks diatomite, agricultural chemicals is adsorbed completely by it.Diatomite after immersion is placed in fume hood after volatilizing, admixes and be ready to
Rural area soil in so that the concentration of Buprofezin and Biphenthrin is respectively 10mgkg in soil sample-1.The quality of every part of soil sample is
500g, water content maintain 35%, are placed in incubated under 30 DEG C of dark conditions.Seed liquor is accessed into soil sample, mixes, makes
The concentration for obtaining the bacterial strain D-6 in soil reaches 108Individual every gram of soil of cell, the soil not connect bacterium are used as control.After cultivating 20d,
The residual quantity of Buprofezin and Biphenthrin in soil sample is measured by HPLC.Measurement result is shown in Table 1, and bacterial strain D-6 in soil to remaining
Buprofezin degradation efficiency reach 95.6%, 73.5% is reached to the Biphenthrin degradation efficiency remained in soil.
It is prepared by the microbial inoculum of embodiment 5
The Buprofezin of the present invention, Biphenthrin degradation bacteria strains D-6 original seed are activated on culture dish, and determine degradability
Can, it is inoculated in standby on test tube slant.Test tube kind is inoculated in culture medium (the LB culture medium prescriptions of LB containing 200mL：Peptone 10g,
Dusty yeast 5g, sodium chloride 5g, water 1L, pH 7.4) 1000mL shaking flasks in, constant-temperature shaking culture to logarithmic phase, prepare inoculation one
Level seeding tank.First class seed pot 50L, inventory 40L, culture medium prescription are：Glucose 0.8%, (NH4)2SO41%, K2HPO4
0.2%, MgSO40.05%, NaCl 0.01%, CaCO30.3%, yeast extract 0.02%, pH value 7.2-7.5.After feeding intake
121 DEG C of high pressure moist heat sterilizations, after being cooled to 30 DEG C, above-mentioned cultured shaking flask strain is inoculated with into 50L by 10% inoculum concentration
First class seed pot, cultivate to exponential phase, mixing speed is 220 revs/min, and filtrated air intake is 1:0.6-1.2.Will
The seed liquor for reaching logarithmic phase accesses secondary seed tank by 10% inoculum concentration.Secondary seed tank 500L, inventory 400L, culture
Based formulas and condition of culture are consistent with first class seed pot.The seed liquor for reaching logarithmic phase is produced into tank by 10% inoculum concentration access
Culture, production tank used medium composition are identical with seed tank culture base.Produce 5 tons of tankage size, 4.5 tons of inventory.After feeding intake
Production tank 1.1kg/cm2 pressure under, 121 DEG C of high pressure moist heat sterilizations, 30 DEG C are cooled to after sterilizing, lead to filtrated air and keep nothing
Bacterium state is standby.At 30 DEG C, the throughput for producing filtrated air in the incubation of tank is for production tank temperature control after inoculation
1:0.6-1.2, mixing speed are 180-240 revs/min, and whole technological process incubation time is 96-108 hours.Fermentation ends
Thalline quantity reaches 1,000,000,000/mL afterwards.
Nutrient solution goes out tank and is directly distributed into liquid dosage form or using peat with plastic barrel or Packaging Bottle after the completion of fermentation
Absorption is distributed into solid fungicide formulation with packaging bag.
Claims (7)
1. the degradation bacteria strains D-6 of a strain insect disinfestations agent Buprofezin and Biphenthrin, it is characterised in that during September was preserved on the 25th in 2017
State's Type Tissue Collection, culture presevation number are CCTCC NO.M 2017537.
2. applications of the degradation bacteria strains D-6 in degraded insecticide Buprofezin and Biphenthrin described in claim 1.
3. degradation bacteria strains D-6 the answering in the microbial inoculum of degraded insecticide Buprofezin and Biphenthrin is prepared described in claim 1
With.
A kind of 4. degradation bacterial agent that degradation bacteria strains D-6 with described in claim 1 is produced.
5. degradation bacterial agent according to claim 4, it is characterised in that described degradation bacterial agent is to produce by the following method
Form：
1) the degradation bacteria strains D-6 test tube kinds described in claim 1 are inoculated in LB culture media shaking vases, shaken cultivation to logarithm
Phase；
2) above-mentioned cultured strain is inoculated with into seeding tank by 10% inoculum concentration, cultivated to exponential phase, seeding tank institute
Culture medium prescription is：Glucose 0.8%, (NH4)2SO41%, K2HPO40.2%, MgSO40.05%, NaCl
0.01%, CaCO30.3%, yeast extract 0.02%, pH value 7.2-7.5；
3) seed liquor is produced into tank used medium and seed tank culture base phase by 10% inoculum concentration access production tank culture
Together；
4) nutrient solution is gone out tank and is directly distributed into liquid preparation with plastic barrel or Packaging Bottle or is inhaled using peat after the completion of fermenting
It is attached to be distributed into solid fungicide preparation with packaging bag；
Wherein, the throughput of filtrated air is volume ratio 1 in the incubation of seeding tank and production tank：0.6-1.2, stirring
Speed is 180-240 revs/min, and cultivation temperature is 30 DEG C, and whole process incubation time is 96-108 hours, thalline after fermentation ends
Quantity reaches 1,000,000,000/more than ml.
6. application of the microbial inoculum in degraded insecticide Buprofezin and Biphenthrin described in claim 5.
7. application according to claim 6, it is characterised in that what the microbial inoculum described in claim 5 remained in soil of degrading
Application in insecticide Buprofezin and Biphenthrin.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110172430A (en) * | 2019-06-18 | 2019-08-27 | 江苏省农业科学院 | One plant of mulberry enterobacteria and its application in degradation Diacloden |
| CN111394274A (en) * | 2017-11-03 | 2020-07-10 | 南京农业大学 | Microbial inoculum for degrading pesticides buprofezin and bifenthrin and application thereof |
| CN114990019A (en) * | 2022-06-16 | 2022-09-02 | 上海市农业科学院 | Organic pollution degrading strain A7, microbial inoculum produced by same and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111440754B (en) * | 2020-03-19 | 2022-05-27 | 南京农业大学 | Method for eliminating organic pollutant residue in soil by using genetically engineered methane-oxidizing bacteria |
| RU2764119C1 (en) * | 2020-11-27 | 2022-01-13 | Общество с ограниченной ответственностью "Органик парк" | STRAIN OF BACTERIA RHODOCOCCUS QINGSHENGII Ac-2143 - DESTRUCTOR OF THE IMAZETHAPYR HERBICIDE AND STIMULATOR OF PLANT GROWTH |
| CN112481170B (en) * | 2020-12-10 | 2023-04-11 | 常州大学 | Pymetrozine degrading bacterium IURM F18 and application thereof |
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| CN105420172A (en) * | 2016-01-08 | 2016-03-23 | 南京农业大学 | Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria |
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| CN101560482B (en) * | 2009-05-22 | 2010-11-03 | 南京农业大学 | Buprofezin pesticide residual degrading bacteria and produced microbial inoculum thereof |
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| AR103287A1 (en) * | 2014-12-29 | 2017-04-26 | Fmc Corp | MICROBIAL COMPOSITIONS AND METHODS TO USE TO BENEFIT THE GROWTH OF PLANTS AND TREAT PLANT DISEASE |
| CN111394274B (en) * | 2017-11-03 | 2021-10-22 | 南京农业大学 | Microbial inoculum for degrading pesticides buprofezin and bifenthrin and application thereof |
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| CN105420172A (en) * | 2016-01-08 | 2016-03-23 | 南京农业大学 | Metribuzin pesticide residue degrading bacteria, microbial agent produced through same and application of metribuzin pesticide residue degrading bacteria |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111394274A (en) * | 2017-11-03 | 2020-07-10 | 南京农业大学 | Microbial inoculum for degrading pesticides buprofezin and bifenthrin and application thereof |
| CN111394274B (en) * | 2017-11-03 | 2021-10-22 | 南京农业大学 | Microbial inoculum for degrading pesticides buprofezin and bifenthrin and application thereof |
| CN110172430A (en) * | 2019-06-18 | 2019-08-27 | 江苏省农业科学院 | One plant of mulberry enterobacteria and its application in degradation Diacloden |
| CN114990019A (en) * | 2022-06-16 | 2022-09-02 | 上海市农业科学院 | Organic pollution degrading strain A7, microbial inoculum produced by same and application thereof |
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| CN111394274B (en) | 2021-10-22 |
| CN107699523B (en) | 2020-06-05 |
| CN111394274A (en) | 2020-07-10 |
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