CN109965110A - A kind of feed addictive preventing cow subclinical mastitis - Google Patents
A kind of feed addictive preventing cow subclinical mastitis Download PDFInfo
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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Abstract
The invention discloses a kind of feed addictive for preventing cow subclinical mastitis, ingredient includes: wheat bran, dregs of beans, corn flour, brown sugar, traditional Chinese medicinal components, precipitated calcium carbonate, yeast extract and fulvic acid；Its each ingredient Zhan total mass ratio are as follows: wheat bran, 60~70 parts；Dregs of beans, 15~20 parts；Corn flour, 10~15 parts；Brown sugar, 2~3 parts；Traditional Chinese medicinal components, 2~3 parts；Precipitated calcium carbonate, 2~4 parts；Yeast extract, 0.5~0.8 part；Fulvic acid, 0.2~0.5 part.The implementation of the embodiment of the present invention can greatly improve the strain activity of active probiotic and the field planting performance in enteron aisle, and make feed addictive as made from it rich in traditional Chinese medicine ingredients, the active constituent for playing pharmacological action in traditional Chinese medicine ingredients can also be made to give full play to, performance and growth promotion performance so that the strong body for improving feed additive for dairy cows is cured the disease, cow subclinical mastitis is effectively treated and prevented, and then replaces the use of antibiotic completely.
Description
Technical field
It is specifically a kind of to prevent milk cow recessive the present invention relates to a kind of Chinese materia medica preparation for preventing cow subclinical mastitis
The feed addictive of mammitis.
Background technique
Cow subclinical mastitis is a kind of disease that is common, complicated, causing dairy economic loss most serious, not only
Utilization periods, milk production and the dairy food quality of cow are seriously affected, and is harmful to food hygiene and human health
Chinese herbal medicine has huge potentiality in terms of preventing and treating mastitis for milk cows.Currently, there are many products to occur, some medium-height grass
Medicine compound preparation and Chinese herbal medicine extract preparation have played important function in terms of the prevention and treatment of mastitis for milk cows, and to treatment
The mechanism of action of mammitis Chinese herbal and crude drugs preparations is studied, it is believed that Chinese herbal treatment mammitis is non-specific by improving body
Property immune function and antibacterial functions are realized.Therefore, carry out the not still sustainable animal husbandry of herbal control mastitis for milk cows
Requirement of the requirement and people of development to improving the quality of living and green food is consumed.
Modern probiotics are with its unique mechanism of action and nontoxic, without side-effects, noresidue, superior without drug resistance etc.
Property, increasingly by the concern of common people, a large amount of organic acid (lactic acid, propionic acid), peptides etc. are generated in microbial fermentation, to machine
Body immune system also functions to facilitation.Therefore, we both improve the active constituent in Chinese medicine using beneficial bacterium come fermented tcm
The performance of pharmacological action, while the probiotics turned out using Chinese medicine as matrix, activity are turned out prebiotic higher than other culture mediums
Bacterium, and enhance colonization ability of the beneficial bacterium in enteron aisle.Since Chinese medicine and probiotics are to the synergistic effect of immune system,
Its immunocompetence of additive of probiotics fermention Chinese medicine production is considerably beyond other class products.We are by by modern microecology
Principle is combined with ancient Chinese medicine, with the Chinese medicinal formulae of probiotics fermention prevention ox recessive mastitis, is produced a kind of complete
Replace the micro- uncooked feed additive of no drug residue, prophyiaxis and promoting growth, low-cost Chinese medicine of antibiotic.
Summary of the invention
The purpose of the present invention aims to solve the problem that the deficiency of above-mentioned technical problem, provides a kind of fermentation with probiotics degradation Chinese medicine
Technology produces the Chinese materia medica preparation of prevention cow subclinical mastitis using more strain fermentation Chinese medicines, i.e., prevents milk with probiotics fermention
The Chinese medicinal formulae of ox recessive mastitis produces a kind of no drug residue of antibiotic of substitution completely, the Chinese materia medica preparation of prophyiaxis and promoting growth.
Its main feature is that being not likely to produce drug resistance, metabolism is very fast in vivo, high-efficiency low-toxicity, noresidue.Alternative chemical synthetic drug, has medicine concurrently
Object and nutritional agents dual function, both can restraining and sterilizing bacteria, enhance the immune function of animal body, add but also as Specialty bio activity
Add agent to adjust the metabolism of body, promotes the absorption of nutriment.
The technical scheme adopted by the invention is that: Tiny ecosystem Chinese materia medica preparation is produced with more strain fermentations, the fermentation preparation
Contain traditional Chinese medicinal components in culture medium, the traditional Chinese medicinal components are by white peony root, Radix Astragali, Radix Codonopsis, honeysuckle, Radix Angelicae Sinensis, rhizome of chuanxiong, the seed of cowherb, road
Lu Tong, radix bupleuri, motherwort, thallus laminariae and Radix Glycyrrhizae composition, the parts by weight of various Chinese medicines are: 60 ~ 80 parts of Radix Astragali, 60 ~ 80 parts of Radix Codonopsis,
60 ~ 80 parts of honeysuckle, radix scutellariae 40 ~ 60,10 ~ 20 parts of rhizome of chuanxiong, 40 ~ 60 parts of the seed of cowherb, 40 ~ 60 parts of Fructus Liquidambaris, 50 ~ 80 parts of radix bupleuri,
40 ~ 60 parts of Radix Angelicae Sinensis, 50 ~ 80 parts of motherwort, 30 ~ 50 parts of thallus laminariae 80-120 Radix Glycyrrhizae,.
The present invention, the parts by weight and additional proportion of each raw material of Chinese herbal medicine are: 80 parts of Radix Astragali, 80 parts of Radix Codonopsis, and honeysuckle
80 parts, 50 parts of radix scutellariae, 20 parts of rhizome of chuanxiong, the seed of cowherb 50,50 parts of Fructus Liquidambaris, 50 parts of radix bupleuri, 60 parts of Radix Angelicae Sinensis, 60 parts of motherwort, elder brother
120 parts of cloth, 50 parts of Radix Glycyrrhizae.
The drug matching that the present invention uses is using monarch, principle minister, helped, made.Radix Astragali is selected first, Radix Codonopsis, Radix Angelicae Sinensis, makees monarch
Medicine improves animal body immunity, achievees the purpose that benefiting qi and nourishing blood: while it is disease-resistant to select honeysuckle, radix scutellariae, radix bupleuri to improve its antibacterial
Malicious ability: it selects rhizome of chuanxiong, the seed of cowherb, Fructus Liquidambaris, motherwort, thallus laminariae activating microcirculation and removing stasis medicinal, promote lactation and improve the output of milk.Select Radix Glycyrrhizae
Not only had the function of clearing heat and detoxicating but also coordinating the drug actions of a prescription.
The function of each drug of traditional Chinese medicinal components of the present invention are as follows: Radix Astragali warm-natured, tonifying Qi and lifting yang sweet in flavor, active constituent are yellow
Immunity of organisms can be improved in astragalus polysaccharides；Radix Codonopsis mild-natured, tonifying middle-Jiao and Qi sweet in flavor and taste, active constituent saponin can improve body
Immunity；Radix Angelicae Sinensis is sweet in flavor warm-natured, can enrich blood and invigorate blood circulation, and active constituent sodium ferulate energy activating macrophage plays and exempts from
Epidemic disease function；Radix Glycyrrhizae has clearing heat and detoxicating, coordinating the drug actions of a prescription, and active constituent glycyrrhizic acid is cell immunomodulator；The mild-natured taste of honeysuckle
It is sweet can be clearing heat and detoxicating, the ingredients such as dispersing swelling and dissipating binds sterol containing honeysuckle, choline, synanthrin and radix scutellariae inhibit mammitis harmful bacteria (gold jointly
Staphylococcus aureus and micrococcus scarlatinae).Rhizome of chuanxiong blood-activating and qi-promoting, yin-nourishing, stagnation resolvation.Rhizome of chuanxiong be blood in gas medicine, allow blood mend and
It is not stagnant.Seed of cowherb Huo Xue Tong Longitude, lower cream disappear carbuncle, contain triterpenoid saponin, glycosides displayed.Fructus Liquidambaris activating microcirculation and removing stasis medicinal, dispelling wind and removing obstruction in the meridians,
Sharp Shui Tong Longitude.Thallus laminariae enriching yin calming the liver, suppression gas dissipating bind, organic content of iodine is high and milk production of cow can be improved rich in the factor is promoted lactation.Benefit
Brittle Falsepimpernel Herb promoting blood circulation contains a variety of alkaloids and organic selenium, the immunity of body can be improved.Radix bupleuri bitter, cold nature.Return liver, gallbladder
Through.There are easypro liver stomach function regulating, the function of rising Yang, anti-bacteria and anti-virus simultaneously has immunoloregulation function.
A kind of feed addictive preventing cow subclinical mastitis
(1) multiple bacteria compound fermentation Chinese medicine, the strain of use are as follows: bacillus subtilis, bafillus natto, lichens gemma are used
Bacillus, brewer's yeast, Chan ?Candida, aspergillus niger, Rhizopus oryzae, lactobacillus plantarum, lactobacillus acidophilus and Bifidobacterium.
(2), by bacillus subtilis, bafillus natto and the bacillus licheniformis after purification respectively from inclined-plane access three
In the bottle of angle, shaken cultivation 24 hours；
(3), by the brewer's yeast, Chan after purification ?Candida, Rhizopus oryzae and aspergillus niger respectively from inclined-plane access triangular flask in,
Shaken cultivation 48 hours；
(4), lactobacillus plantarum, lactobacillus acidophilus and the Bifidobacterium after purification are accessed in Kolle flask from inclined-plane respectively, temperature
37 DEG C of control is cultivated 24 hours；
(5) above-mentioned cultured 10 kinds of bacterium are mixed into (every kind of bacterium mixed proportion 10%) and is inoculated into seed fermenter by 3% -5%.Kind
Sub- tank culture medium uses brown sugar, potassium dihydrogen phosphate, yeast extract, peptone.Each component ratio are as follows: brown sugar 3-5%, potassium dihydrogen phosphate
0.2-0.5%, yeast extract 0.3-0.5%, peptone 0.3-0.5%, surplus are water, and pH is natural.At 121 DEG C, sterilize 25 minutes,
Inoculation when being cooled to 30 DEG C, inoculum concentration are 3 ~ 5%；Using anaerobic fermentation, revolving speed 180r/min, 32 DEG C of temperature, fermentation time 48
~ 72 hours, when pH value reaches 4.8, it was fermentation termination that viable count, which reaches 1,500,000,000/ml,.
(6), cultured seed liquor is inoculated into herb fermenting tank by 5-10%.Fermented tcm culture medium is using red
Sugar, traditional Chinese medicinal components, potassium dihydrogen phosphate, yeast extract, each Composition Weight Parts and additional proportion are: brown sugar 5-8%, traditional Chinese medicinal components 2
~ 3%, potassium dihydrogen phosphate 0.2-3%, yeast extract 0.3-0.5%, surplus is water, and pH value is naturally, temperature control is gone out at 121 DEG C
Bacterium 20 minutes, inoculation when being cooled to 30 DEG C, inoculum concentration was 5 ~ 10%；Using anaerobic fermentation, revolving speed 180r/min, 32 DEG C of temperature,
Fermentation time 48 ~ 72 hours, when pH value reaches 5.0, it was fermentation termination that viable count, which reaches 2,000,000,000/ml,.
(7) the above-mentioned fermentation liquid containing Chinese medicine fermented is mixed by the maize cob meal of 1:1 and microwave sterilization as solid-state
The solid-state seed of fermentation.
(8) solid-state seed is inoculated into solid medium by 20-30%.Solid fermentation Chinese-medicinal culture medium using wheat bran,
Dregs of beans, corn flour, brown sugar, precipitated calcium carbonate, traditional Chinese medicinal components, yeast extract, fulvic acid, each Ingredients Weight score and additional proportion
It is: wheat bran 60-70%, dregs of beans 15-20, corn flour 10-15, brown sugar 2-3%, precipitated calcium carbonate 2-4%, traditional Chinese medicinal components 2-
3%, yeast extract 0.5-0.8%, fulvic acid 0.2-0.5%, each Ingredients Weight score and additional proportion are: wheat bran 65%, dregs of beans 15,
Corn flour 10, brown sugar 2%, precipitated calcium carbonate 2.5%, traditional Chinese medicinal components 2%, yeast extract 0.5%, fulvic acid 0.2%.Moisture is adjusted to 35%.
It stirs evenly and is inoculated with after microwave sterilization.It is sealed by fermentation in the greenhouse, temperature is kept for 37 degree.When temperature rises to 40 degree of Shi Jinhang
It overturns.Fermentation time 5-7 days.When viable count reaches 4,500,000,000 or more, temperature is not fermentation termination when rising.The object fermented
Material after screening machine by packing.
The beneficial effects of the present invention are: the traditional Chinese medicinal components different with probiotics fermention, are a kind of antibiotic of substitution completely
No drug residue, prophyiaxis and promoting growth, low-cost Chinese materia medica preparation.Since Chinese medicine and probiotics are to the synergistic effect of immune system, because
Its immunocompetence of additive of this probiotics fermention Chinese medicine production is considerably beyond other class products.
Specific embodiment
The parts by weight of various Chinese medicines are: 60 ~ 80 parts of Radix Astragali, 60 ~ 80 parts of Radix Codonopsis, and 60 ~ 80 parts of honeysuckle, radix scutellariae 40 ~ 60, river
10 ~ 20 parts of vault, 40 ~ 60 parts of the seed of cowherb, 40 ~ 60 parts of Fructus Liquidambaris, 50 ~ 80 parts of radix bupleuri, 40 ~ 60 parts of Radix Angelicae Sinensis, motherwort 50 ~ 80
Part, 30 ~ 50 parts of thallus laminariae 80-120, Radix Glycyrrhizae.
The present invention, the parts by weight and additional proportion of each raw material of Chinese herbal medicine are 80 parts of Radix Astragali, 80 parts of Radix Codonopsis, honeysuckle 80
Part, 50 parts of radix scutellariae, 20 parts of rhizome of chuanxiong, the seed of cowherb 50,50 parts of Fructus Liquidambaris, 50 parts of radix bupleuri, 60 parts of Radix Angelicae Sinensis, 60 parts of motherwort, thallus laminariae
120 parts, 50 parts of Radix Glycyrrhizae.
The culture of the microorganism original seed of fermented tcm and the culture of seed liquor: by the bacillus subtilis after purification, natto
Bacillus and bacillus licheniformis are respectively connected to shaken cultivation 24 hours in triangular flask from inclined-plane；By the beer ferment after purification
Female, Chan ?Candida, Rhizopus oryzae and aspergillus niger, respectively from inclined-plane access triangular flask shaken cultivation 48 hours；After purification
Lactobacillus casei, lactobacillus acidophilus and Bifidobacterium, respectively from inclined-plane access Kolle flask, temperature controls the culture 24 at 37 DEG C
Hour.Above-mentioned cultured 10 kinds of bacterium are mixed into (every kind of bacterium mixed proportion 10%) and are inoculated into seed fermenter by 3%.Seeding tank training
It supports base and uses brown sugar, potassium dihydrogen phosphate, yeast extract, peptone.Each component ratio are as follows: brown sugar 8%, potassium dihydrogen phosphate 0.2%, yeast
Cream 0.5%, peptone 0.3%, surplus are water, and pH is natural.At 121 DEG C, sterilize 25 minutes, inoculation when being cooled to 30 DEG C；Using detesting
Aerobe fermentation, revolving speed 180r/min, 32 DEG C of temperature, fermentation time 48 ~ 72 hours, when pH value reaches 4.8, viable count reached 12
Hundred million/ml is fermentation termination.
Cultured seed liquor is inoculated into herb fermenting tank by 8%.Fermented tcm culture medium uses brown sugar, Chinese medicine group
Divide, potassium dihydrogen phosphate, yeast extract, each Composition Weight Parts and additional proportion are: brown sugar 6%, traditional Chinese medicinal components 3%, biphosphate
Potassium 0.2%, yeast extract 0.5%, surplus are water, and pH value is naturally, temperature control sterilizes 20 minutes, when being cooled to 30 DEG C at 121 DEG C
Inoculation；Using anaerobic fermentation, revolving speed 180r/min is 32 DEG C of temperature, fermentation time 48 ~ 72 hours, living when pH value reaches 5.0
It is fermentation termination that bacterium number, which reaches 2,000,000,000/ml,.
The above-mentioned fermentation liquid containing Chinese medicine fermented is mixed by the maize cob meal of 1:1 and microwave sterilization as solid state fermentation
Solid-state seed.
Solid-state seed is inoculated into solid medium by 20-30%.Solid fermentation Chinese-medicinal culture medium uses wheat bran, beans
The dregs of rice, corn flour, brown sugar, precipitated calcium carbonate, traditional Chinese medicinal components, yeast extract, kelp, fulvic acid, each Ingredients Weight score and addition ratio
Example is: wheat bran 60-70%, dregs of beans 15-20, corn flour 10-15, brown sugar 2-3%, precipitated calcium carbonate 2-4%, traditional Chinese medicinal components
2-3%, yeast extract 0.5-0.8%, fulvic acid 0.2-0.5%, each Ingredients Weight score and additional proportion are:, wheat bran 65%, beans
The dregs of rice 15, corn flour 10, brown sugar 2%, precipitated calcium carbonate 2.5%, traditional Chinese medicinal components 2%, yeast extract 0.5%, fulvic acid 0.2%.Moisture is adjusted to
35%.It stirs evenly and is inoculated with after microwave sterilization.It is sealed by fermentation in the greenhouse, temperature is kept for 37 degree.When temperature rises to 40 degree
It is overturned.Fermentation time 5-7 days.When viable count reaches 4,500,000,000 or more, temperature is not fermentation termination when rising.It ferments
Material by packing after screening machine.
Claims (7)
1. contain traditional Chinese medicinal components in a kind of culture medium for the feed addictive fermentation preparation for preventing cow subclinical mastitis, it is described
Traditional Chinese medicinal components are by white peony root, Radix Astragali, Radix Codonopsis, honeysuckle, Radix Angelicae Sinensis, rhizome of chuanxiong, the seed of cowherb, Fructus Liquidambaris, radix bupleuri, motherwort, thallus laminariae and sweet
Grass composition, the parts by weight of various Chinese medicines are: 60 ~ 80 parts of Radix Astragali, 60 ~ 80 parts of Radix Codonopsis, and 60 ~ 80 parts of honeysuckle, radix scutellariae 40 ~ 60, river
10 ~ 20 parts of vault, 40 ~ 60 parts of the seed of cowherb, 40 ~ 60 parts of Fructus Liquidambaris, 50 ~ 80 parts of radix bupleuri, 40 ~ 60 parts of Radix Angelicae Sinensis, motherwort 50 ~ 80
Part, 30 ~ 50 parts of thallus laminariae 80-120 Radix Glycyrrhizae.
2. a kind of feed addictive for preventing cow subclinical mastitis as described in claim 1 is it is characterized by: the medium-height grass
The parts by weight and additional proportion of each raw material of medicine are: 80 parts of Radix Astragali, 80 parts of Radix Codonopsis, and 80 parts of honeysuckle, 50 parts of radix scutellariae, 20 parts of rhizome of chuanxiong,
The seed of cowherb 50,50 parts of Fructus Liquidambaris, 50 parts of radix bupleuri, 60 parts of Radix Angelicae Sinensis, 60 parts of motherwort, 120 parts of thallus laminariae, 50 parts of Radix Glycyrrhizae.
3. a kind of feed addictive for preventing cow subclinical mastitis as described in claim 1 it is characterized by:
(1) multiple bacteria compound fermentation Chinese medicine, the strain of use are as follows: bacillus subtilis, bafillus natto, lichens gemma are used
Bacillus, brewer's yeast, Chan ?Candida, aspergillus niger, Rhizopus oryzae, lactobacillus plantarum, lactobacillus acidophilus and Bifidobacterium；
(2), bacillus subtilis, bafillus natto and the bacillus licheniformis after purification are accessed into triangular flask from inclined-plane respectively
In, shaken cultivation 24 hours；
(3), by the brewer's yeast, Chan after purification ?Candida, Rhizopus oryzae and aspergillus niger respectively from inclined-plane access triangular flask in,
Shaken cultivation 48 hours；
(4), lactobacillus plantarum, lactobacillus acidophilus and the Bifidobacterium after purification are accessed in Kolle flask from inclined-plane respectively, temperature
37 DEG C of control is cultivated 24 hours；
(5) above-mentioned cultured 10 kinds of bacterium are mixed into (every kind of bacterium mixed proportion 10%) and is inoculated into seed fermenter by 3% -5%；
Seed tank culture base uses brown sugar, potassium dihydrogen phosphate, yeast extract, peptone；
Each component ratio are as follows: brown sugar 3-5%, potassium dihydrogen phosphate 0.2-0.5%, yeast extract 0.3-0.5%, peptone 0.3-
0.5%, surplus is water, and pH is natural；
It at 121 DEG C, sterilizes 25 minutes, inoculation when being cooled to 30 DEG C, inoculum concentration is 3 ~ 5%；Using anaerobic fermentation, revolving speed 180r/
Min, 32 DEG C of temperature, fermentation time 48 ~ 72 hours, when pH value reaches 4.8, it was fermentation termination that viable count, which reaches 1,500,000,000/ml,；
(6), cultured seed liquor is inoculated into herb fermenting tank by 5-10；
Fermented tcm culture medium uses brown sugar, traditional Chinese medicinal components, potassium dihydrogen phosphate, yeast extract, each Composition Weight Parts and addition ratio
Example is: brown sugar 3 ~ 5%, traditional Chinese medicinal components 1 ~ 2%, potassium dihydrogen phosphate 0.2-3%, yeast extract 0.3-0.5%, surplus are water, PH
For value naturally, temperature control sterilizes 20 minutes at 121 DEG C, inoculation when being cooled to 30 DEG C, inoculum concentration is 5 ~ 10%；It is sent out using anaerobism
Ferment, revolving speed 180r/min, 32 DEG C of temperature, fermentation time 48 ~ 72 hours, when pH value reaches 5.0, viable count reached 2,000,000,000/ml
As fermentation termination；
(7) the above-mentioned fermentation liquid containing Chinese medicine fermented is mixed by the maize cob meal of 1:1 and microwave sterilization as solid state fermentation
Solid-state seed；
(8) solid-state seed is inoculated into solid medium by 20-30%.
4. solid fermentation Chinese-medicinal culture medium using wheat bran, dregs of beans, corn flour, brown sugar, precipitated calcium carbonate, traditional Chinese medicinal components, yeast extract,
Kelp, fulvic acid, each Ingredients Weight score and additional proportion are: wheat bran 60-70%, dregs of beans 15-20, corn flour 10-15, red
Sugared 2-3%, precipitated calcium carbonate 2-4%, traditional Chinese medicinal components 2-3%, yeast extract 0.5-0.8%, fulvic acid 0.2-0.5%, each ingredient
Weight fraction and additional proportion are:
Wheat bran 65%, dregs of beans 15, corn flour 10, brown sugar 2%, precipitated calcium carbonate 2.5%, traditional Chinese medicinal components 2%, yeast extract 0.5%, yellow corruption
Acid 0.2%；Moisture is adjusted to 35%.
It is inoculated with after microwave sterilization 5. stirring evenly.
6. being sealed by fermentation in the greenhouse, temperature is kept for 37 degree, is overturned when temperature rises to 40 degree, fermentation time 5-7
It, when viable count reaches 4,500,000,000 or more, temperature is not fermentation termination when rising.
7. the material fermented is packed after passing through screening machine.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110771743A (en) * | 2019-10-25 | 2020-02-11 | 曹文飞 | Feed capable of preventing dairy cow mastitis and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110771743A (en) * | 2019-10-25 | 2020-02-11 | 曹文飞 | Feed capable of preventing dairy cow mastitis and preparation method thereof |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20190705 |