CN109946460A - Chemiluminescence immunoassay detection device and application method based on micro-fluidic chip - Google Patents
Chemiluminescence immunoassay detection device and application method based on micro-fluidic chip Download PDFInfo
- Publication number
- CN109946460A CN109946460A CN201910313140.7A CN201910313140A CN109946460A CN 109946460 A CN109946460 A CN 109946460A CN 201910313140 A CN201910313140 A CN 201910313140A CN 109946460 A CN109946460 A CN 109946460A
- Authority
- CN
- China
- Prior art keywords
- micro
- fluidic chip
- reaction chamber
- electric machine
- rotating electric
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The present invention provides chemiluminescence immunoassay detection devices and application method based on micro-fluidic chip, belong to biochemical investigation apparatus field, including reaction chamber, it is arranged in the reaction chamber and by the micro-fluidic chip of rotating electric machine driving rotation, lifting assembly, for driving the oscilaltion of rotating electric machine, realize chip washing, reaction, acquire the switching of position, magnet ring, lower end inside the reaction chamber is set, absorption magnetic bead provides continuous magnetic attraction when for chip high speed rotation, acquisition module, for acquiring optical signal and uploading to control panel, temperature control component, for controlling the temperature of reaction chamber, meet enzyme reaction demand, control panel, control acquisition module, the movement of temperature control component and rotating electric machine, control the rotation and stopping of micro-fluidic chip.Degree of integration of the present invention is high, easy to operate, realizes full-automatic detection.
Description
Technical field
The invention belongs to biochemical investigation apparatus field, be related to chemiluminescence immunoassay detection device based on micro-fluidic chip and
Application method.
Background technique
Microfluidic chip technology is that accurate manipulation and control nanoliter and picoliters magnitude fluid are (raw in the runner of micro-meter scale
Object sample fluid) new technology, using this technology can sample preparation involved in chemistry and the fields such as biology, reaction,
The basic operation units such as separation, detection and cell culture, sorting, cracking are integrated or are integrated into one piece several square centimeters substantially (very
To smaller) chip on, network is formed by fluid channel, whole system is run through with controllable fluid, to replace conventional chemical or
A kind of technology platform of the various functions of biology laboratory.The essential characteristic and sharpest edges of Microfluid based Lab on a chip are a variety of lists
First technology flexible combination, scale on whole controllable small platform is integrated.
Chemiluminescence immune assay (chemiluminescence immunoassay, CLIA), is will have high sensitivity
Chemical luminescent detecting technology combined with the immune response of high specific, for various antigens, haptens, antibody, hormone,
The detection and analysis technology of enzyme, fatty acid, vitamin and drug etc..Be after radioimmunology analysis, enzyme exempt from analysis, fluoroimmunoassay and when
Between a newest immunoassay growing up after resolved fluorometric immunoassay.
Chemiluminescence immunoassay is divided into following three kinds substantially:
1) the direct chemiluminescence of, marker are acridinium ester；
2) enzyme-catalyzed chemical luminescence, marker are alkaline phosphatase or horseradish peroxidase；
3) electrochemical luminescence, marker are tris (bipyridine) ruthenium；
At present be not based on micro-fluidic chip on the market and entry simultaneously detect relevant device, progress double antibody folder
After the production of heart compound, during unbonded enzyme labelled antibody is removed in washing, the method that tradition large size detection device uses is more
Secondary absorption-dilution-absorption mode, process is complicated and generates more waste liquid.
Summary of the invention
The problem to be solved in the present invention is the chemiluminescence immunoassay detection device for being to provide based on micro-fluidic chip and makes
With method, degree of integration is high, easy to operate, realizes full-automatic detection.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: the chemiluminescence based on micro-fluidic chip is exempted from
Epidemic disease detection device, including reaction chamber are arranged in the reaction chamber and by the micro-fluidic chip of rotating electric machine driving rotation；
Lifting assembly realizes chip washing, reaction, the switching of acquisition position for driving the oscilaltion of rotating electric machine；
The lower end inside the reaction chamber is arranged in magnet ring, and absorption magnetic bead provides continuous when for chip high speed rotation
Magnetic attraction；
Acquisition module, for acquiring optical signal and uploading to control panel；
Temperature control component meets enzyme reaction demand for controlling the temperature of reaction chamber；
Control panel controls the movement of acquisition module, temperature control component and rotating electric machine, controls the rotation of micro-fluidic chip
And stopping.
Further, the lifting assembly is scissors lift platform structure, and the lifting assembly includes that upper end is horizontally disposed
Lifting platform, the rotating electric machine is installed on the lifting platform, and the lifting assembly is driven by lifting motor, the lifting electricity
Machine controls its rotation by control panel.
Further, the structure of the lifting assembly also packet pedestal, lifting platform described in the structure of the pedestal is identical and two
Person's setting symmetrical above and below, be equipped between the pedestal and the lifting platform formed the first connecting rods of cutting assemblies, second connecting rod and
The lower end of shaft, the cutting assemblies is slidably connected by sliding block and the pedestal, and there are lead screw, silk in the upper end of the cutting assemblies
Mother's driving OQ t platform is slidably connected, and lead screw is driven by lifting motor to be rotated, and screw is by being erected at leading for lifting platform lower end
Bar is slidably connected with lifting platform.
Further, the acquisition module includes balancing stand, collector and sealing element, and the upper end of the reaction chamber is equipped with
Acquisition window, described to balance the upper end for being erected at the sealed chamber, the sealing element is located at the lower end of the balancing stand, described
Sealing element is flexible material, and under acquisition state, the micro-fluidic chip moves up and contacts setting with the sealing element, described close
Sealing is located at the outer ring of the acquisition window, and the collector is located at the upper end of the balancing stand and the corresponding acquisition window is set
It sets.
Further, the upper end of the reaction chamber is set there are three acquisition window, and the lower end of the balancing stand is adopted with described
The corresponding position of collection window is equipped with sealing ring.
Further, the temperature control component includes the U-shaped air duct for being located at reaction chamber rear end and being connected to its inside, described
The one end in U-shaped air duct is as air inlet, and for the other end as air outlet, internal middle part is equipped with heating component, the air inlet and
Air outlet is equipped with aerofoil fan at the reaction chamber, and the temperature control component further includes being arranged in the indoor temperature of reaction chamber
Collector is spent, the temperature sampler is electrically connected with the control panel.
Further, the micro-fluidic chip includes dilution well, sample application hole, detection slot and waste liquid tank, institute
It states dilution well to be connected to sample application Kong Junyu mixing channel, the mixing channel is connected to detection slot, the detection slot
Lower end is equipped with the cleaning solution quantitative slot communicated therewith, and the detection slot is connected to setting with waste liquid tank.
Using the method for the chemiluminescence immunoassay detection device based on micro-fluidic chip, S1: device power-up prepares；
S2: addition detection sample to sample application hole adds dilution to the dilution well of micro-fluidic chip, will add
The micro-fluidic chip added is put into reaction chamber and fixes with rotating electric machine；
S3: after being installed above, in reaction position after rotating electric machine and micro-fluidic chip decline, rotating electric machine drives micro-
Fluidic chip high speed rotation, cooperation micro-fluidic chip are sequentially completed the separation of sample and dilution, quantitative, dilution, heating；It is fixed to make
The mixed liquor of the sample and dilution measured enters the detection slot of micro-fluidic chip, and the magnetic bead antibody pre-installed in detection slot is frozen
Dry bead, enzyme labelled antibody freeze-drying bead dissolve and react it, form double-antibody sandwich compound；
S4: lifting assembly decline makes micro-fluidic chip reach washing position, and magnetic bead is attached to core under the magneticaction of magnet ring
The bottom surface of piece detection slot, rotating electric machine high-speed rotation, the liquid comprising extra enzyme labelled antibody enter useless under the influence of centrifugal force
Liquid bath, and double-antibody sandwich compound is retained in detection slot under the action of magnetic attraction；
S5: lifting assembly rises, and so that magnetic bead is detached from the magnetic force constraint of magnet ring, and discharge the cleaning in cleaning solution quantitative slot
Liquid, motor rotation make cleaning solution enter detection slot, and magnetic bead suspends again；
S6: rotating electric machine and micro-fluidic chip return to reaction position, discharge luminous substrate, and rotation micro-fluidic chip makes the bottom that shines
Object enters detection slot, and cooperation micro-fluidic chip completes the mixing of detection liquid, reaction；
S7: rotating electric machine drives micro-fluidic chip rotation, is aligned detection slot with acquisition module, will be on the optical signal of acquisition
It passes to control panel and carries out quantitative analysis.
Further, the temperature control component controlled at 37 degree ± 0.3 degree.
Further, S5 and between repeat step 4,5,4, washed once with cleaning solution 2 times, thoroughly exclude unbonded enzyme
Labeling antibody completes washing step.
Compared with prior art, the present invention has the advantage that as follows with good effect.
1, the micro-fluidic chip that present invention setting is driven by motor, can be realized determining for liquid under the high-speed rotation of motor
Amount and separation, while magnet ring is set, absorption magnetic bead provides continuous magnetic attraction when for chip high speed rotation, realizes point of liquid
From and cleaning, while give motor configure lifting assembly, realize micro-fluidic chip washing position, reaction position, acquire position freedom
Switching will test the centrifugation of sample, quantitative, dilution, mixing, rinse in one, while acquisition module and temperature control component is arranged, and protect
The temperature environment for demonstrate,proving reaction is constant, determines the accuracy of detection, acquisition component carries out the acquisition and processing of data in time, all
Electrical component is controlled by control panel, high degree of automation, and pilot process is unmanned to be participated in, and is improved working efficiency, is dropped
Low labor intensity, and the precision of detection is improved, degree of integration is high, and it is easy to operate, realize full-automatic detection；
2, the upper end of reaction chamber is set there are three acquisition window, and the lower end of balancing stand position corresponding with acquisition window is all provided with
There is sealing ring, in light signal collection, to avoid chip light leakage due to part is squeezed disequilibrium, influences collection result,
Reaction chamber upper surface increases the acquisition window that the axle center of three opposite micro-fluidic chips is uniformly arranged, supporting point and acquisition module
Symmetric points are formed, to guarantee micro-fluidic chip stress balance；
3, the setting of two aerofoil fans, so that the indoor air of reaction chamber locating for micro-fluidic chip flows, heating
Component is in the intermediate position in U-shaped air duct, the air progress circulating-heating by air duct, temperature under the drive of aerofoil fan
Collector is placed on the indoor front end of reaction chamber, defeated according to heating of the feedback control of temperature sampler to air duct inner circulating air
Ratio out, guarantee the temperature of reaction chamber at 37 degree ± 0.3 degree, good constant-temperature effect.
Detailed description of the invention
The attached drawing for constituting a part of the invention is used to provide further understanding of the present invention, schematic reality of the invention
It applies example and its explanation is used to explain the present invention, do not constitute improper limitations of the present invention.In the accompanying drawings:
Fig. 1 be the present invention relates to enzyme-catalyzed chemical luminescence method reaction principle figure；
Fig. 2 is the structural schematic diagram of the chemiluminescence immunoassay detection device the present invention is based on micro-fluidic chip；
Fig. 3 is the top view of the chemiluminescence immunoassay detection device the present invention is based on micro-fluidic chip；
Fig. 4 is the B-B cross-sectional view of Fig. 3 of the present invention；
Fig. 5 is the portion the A detail drawing of Fig. 4 of the present invention；
Fig. 6 is the front view of the chemiluminescence immunoassay detection device the present invention is based on micro-fluidic chip；
Fig. 7 is the C-C cross-sectional view of Fig. 6 of the present invention；
Fig. 8 is the structural schematic diagram of balancing stand of the present invention；
Fig. 9 is that the present invention is based on the structural representations that the chemiluminescence immunoassay detection device of micro-fluidic chip is in washing position
Figure；
Figure 10 is that the present invention is based on the structural representations that the chemiluminescence immunoassay detection device of micro-fluidic chip is in reaction position
Figure；
Figure 11 is that the present invention is based on the structural representations that the chemiluminescence immunoassay detection device of micro-fluidic chip is in acquisition position
Figure；
Figure 12 is the structural schematic diagram of micro-fluidic chip of the present invention.
Appended drawing reference:
1, reaction chamber；11, acquisition window；2, lifting assembly；21, lifting platform；22, pedestal；23, first connecting rod；24,
Two connecting rods；25, shaft；26, sliding block；27, lead screw；28, screw；3, acquisition module；31, collector；32, balancing stand；33, it seals
Part；4, temperature control component；41, U-shaped air hose；42, aerofoil fan；43, heating component；45, temperature sampler；5, control panel；6,
Rotating electric machine；7, micro-fluidic chip；71, dilution well；72, sample application hole；73, detection slot；74, waste liquid tank；75, it mixes
Close slot；76, luminous substrate reserve tank；77, cleaning solution quantitative slot；8, magnet ring.
Specific embodiment
It should be noted that in the absence of conflict, the feature in embodiment and embodiment in the present invention can phase
Mutually combination.
In the description of the present invention, it is to be understood that, term " center ", " longitudinal direction ", " transverse direction ", "upper", "lower",
The orientation or positional relationship of the instructions such as "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outside" is
It is based on the orientation or positional relationship shown in the drawings, is merely for convenience of description of the present invention and simplification of the description, rather than instruction or dark
Show that signified device or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as pair
Limitation of the invention.In addition, term " first ", " second " etc. are used for description purposes only, it is not understood to indicate or imply phase
To importance or implicitly indicate the quantity of indicated technical characteristic.The feature for defining " first ", " second " etc. as a result, can
To explicitly or implicitly include one or more of the features.In the description of the present invention, unless otherwise indicated, " multiple "
It is meant that two or more.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or be integrally connected；It can
To be mechanical connection, it is also possible to be electrically connected；It can be directly connected, can also can be indirectly connected through an intermediary
Connection inside two elements.For the ordinary skill in the art, above-mentioned term can be understood by concrete condition
Concrete meaning in the present invention.
Specific embodiments of the present invention will now be described in detail with reference to the accompanying drawings.
As shown in Figure 1, enzyme-catalyzed chemical luminescence method reaction principle is as follows: the antibody of magnetic bead, mould labeling antibody will be coated with, containing anti-
Former detection sample is put together reaction a period of time, forms double-antibody sandwich compound, it is anti-that unbonded enzyme mark is removed in washing
Body adds quantitative luminous substrate with mould react and issues the light of specific wavelength, acquires number of photons using photomultiplier tube, with into
Row quantitative analysis.
As shown in Figure 2 to 7, the present invention is the chemiluminescence immunoassay detection device based on micro-fluidic chip, including reaction
Chamber 1 is arranged in reaction chamber 1 and by the micro-fluidic chip 7 of the driving rotation of rotating electric machine 6；
Lifting assembly 2 realizes washing, reaction, the acquisition of micro-fluidic chip 7 for driving the oscilaltion of rotating electric machine 6
The switching of position；
The lower end inside reaction chamber 1 is arranged in magnet ring 8, and magnetic bead is adsorbed when for micro-fluidic die chip high-speed rotation and is mentioned
For continuous magnetic attraction；
Acquisition module 3, for acquiring optical signal and uploading to control panel 5；
Temperature control component 4 meets enzyme reaction demand for controlling the temperature of reaction chamber 1；
Control panel 5 controls the movement of acquisition module 3, temperature control component 4 and rotating electric machine 6, controls micro-fluidic chip 7
Rotation and stopping.
Preferably, lifting assembly 2 is 21 structure of scissors lift platform, and lifting assembly 2 includes the horizontally disposed lifting in upper end
Platform 21, rotating electric machine 6 are installed on lifting platform 21, and lifting assembly 2 is driven by lifting motor, and lifting motor is controlled by control panel 5
Make its rotation, it is preferable that the also packet pedestal 22 of lifting assembly 2, the structure of the structure lifting platform 21 of pedestal 22 is identical and the two above and below
It is symmetrical arranged, the first connecting rod 23, second connecting rod 24 and shaft 25 for forming cutting assemblies is equipped between pedestal 22 and lifting platform 21,
The lower end of cutting assemblies is slidably connected by sliding block 26 with pedestal 22, and the upper end of cutting assemblies has lead screw 27, screw 28 to drive phase
It is slidably connected to lifting platform 21, lead screw 27 is driven by lifting motor to be rotated, and screw 28 is by being erected at leading for 21 lower end of lifting platform
Bar is slidably connected with lifting platform 21, and lifting motor rotation drives lead screw 27 to rotate, and lead screw 27 is equipped with screw 28, is realized
The moving horizontally on guide rod of screw 28, carries out the variation for realizing 24 intersecting angle of first connecting rod 23 and second connecting rod, most
The completion of lifting action is realized eventually, this structure is simple, and reliable and stable, lifting motor is controlled by control panel 5, realizes
The positioning of height different location, stability is high, and control panel 5 is controlled using PLC program, and program is simple, easy to operate.
Preferably, acquisition module 3 includes balancing stand 32, collector 31 and sealing element 33, and the upper end of reaction chamber 1, which is equipped with, adopts
Collect window 11, balancing stand 32 is located at the upper end of sealed chamber, and sealing element 33 is located at the lower end of balancing stand 32, and sealing element 33 is flexibility
Material, under acquisition state, micro-fluidic chip 7, which is moved up, contacts setting with sealing element 33, and sealing element 33 is located at acquisition window 11
Outer ring, collector 31 is located at the upper end of balancing stand 32 and corresponding acquisition window 11 is arranged, and the setting of sealing element 33 ensure that and adopt
Leakproofness under collection state guarantees the efficiency and accuracy of signal acquisition.
Preferably, the upper end of reaction chamber 1 is set there are three acquisition window 11, and the lower end of balancing stand 32 and acquisition window 11 are right
The position answered is equipped with sealing ring, in light signal collection, to avoid chip because being locally squeezed disequilibrium due to light leakage, influences
Collection result, the acquisition window 11 that the axle center of increase by three opposite micro-fluidic chips 7 is uniformly arranged in 1 upper surface of reaction chamber,
Supporting point and acquisition module 3 form symmetric points, to guarantee 7 stress balance of micro-fluidic chip.
Preferably, temperature control component 4 includes the U-shaped air duct for being located at 1 rear end of reaction chamber and being connected to its inside, U-shaped air duct
One end as air inlet, the other end is equipped with heating component 43 as air outlet, internal middle part, and air inlet and air outlet are close
Aerofoil fan 42 is equipped at reaction chamber 1, temperature control component 4 further includes the temperature sampler 4531 being arranged in reaction chamber 1,
Temperature sampler 4531 is electrically connected with control panel 5, the setting of two aerofoil fans 42, so that the locating reaction of micro-fluidic chip 7
Air in chamber 1 flows, and heating component 43 is in the intermediate position in U-shaped air duct, the handle under the drive of aerofoil fan 42
Circulating-heating is carried out by the air in air duct, temperature sampler 4531 is placed on the front end in reaction chamber 1, according to temperature acquisition
The feedback control of device 4531 guarantees the temperature of reaction chamber 1 at 37 degree ± 0.3 the heating export ratio of air duct inner circulating air
Degree, good constant-temperature effect, heating component 43 can be the existing structures such as electric heating sheets.
Preferably, micro-fluidic chip 7 includes dilution well 71, sample application hole 72, detection slot 73 and waste liquid tank 74,
Dilution well 71 and sample application hole 72 are connected to mixing channel 75, and mixing channel 75 is connected to detection slot 73, detection slot 73
Lower end be equipped with the cleaning solution quantitative slot 77 that communicates therewith, detection slot 73 is connected to setting, the lower end of detection slot 73 with waste liquid tank 74
It is additionally provided with the luminous substrate reserve tank 76 communicated therewith, sample and dilution mix in mixing channel 75 first, in the drive of motor
Dynamic lower high speed and not rotation at the uniform velocity, realize the abundant fusion of sample and dilution, then mixed liquid enters
Detection slot 73 realizes secondary fusion reaction under the rotation of micro-fluidic chip 7, forms double-antibody sandwich compound, total
Simply, the principle of centrifugal force is made full use of, and combines different revolving speed and rotation direction, realizes the quantitative and fusion of liquid,
And by the effect of magnet ring 8, cleaning and separation are realized, provide structure basis for the detection of automatic chemistry electrochemiluminescent immunoassay.
In practical work process, follow the steps below, S1: device power-up prepares；S2: addition detection sample to sample
This well 72, the dilution well 71 of addition dilution to micro-fluidic chip 7 put the micro-fluidic chip 7 that addition is completed
Enter in reaction chamber 1 and fixed with motor, as shown in figure 11, above step is that lifting platform 21 is at the highest notch when progress
's；S3: after being installed above, in reaction position, as shown in Figure 10, electric rotating after rotating electric machine 6 and the decline of micro-fluidic chip 7
Machine 6 drives 7 high speed rotation of micro-fluidic chip, and cooperation chip is sequentially completed the separation of sample and dilution, quantitative, dilution, heating；
The mixed liquor of quantitatively complete sample and dilution is set to enter the detection slot 73 of micro-fluidic chip 7, the magnetic pre-installed in detection slot
Pearl antibody freeze-drying bead, enzyme labelled antibody freeze-drying bead dissolve and react it, form double-antibody sandwich compound；S4: it rises
Part 2 of coming down to a lower group decline makes micro-fluidic chip 7 reach washing position, as shown in figure 9, magnetic bead is attached to core under the magneticaction of magnet ring 8
The bottom surface of piece detection slot 73, rotating electric machine high-speed rotation, the liquid comprising extra enzyme labelled antibody enter under the influence of centrifugal force
Waste liquid tank 74, and double-antibody sandwich compound is retained in detection slot 73 under the action of magnetic attraction；S5: lifting assembly 2 rises,
So that magnetic bead is detached from the magnetic force constraint of magnet ring 8, and discharge the cleaning solution in cleaning solution quantitative slot, motor rotation makes cleaning solution enter inspection
Slot 73 is surveyed, magnetic bead suspends again；S6: repeating step 4,5,4, washed once with cleaning solution 2 times, and it is anti-thoroughly to exclude unbonded enzyme mark
Body completes washing step；S7: rotating electric machine and micro-fluidic chip return to reaction position, discharge luminous substrate, rotate micro-fluidic chip
7 make luminous substrate enter detection slot 73, and cooperation micro-fluidic chip 7 completes the mixing of detection liquid, reaction；S8: motor drives miniflow
It controls chip 7 to rotate, is aligned detection slot 73 with acquisition window 11, lifting assembly 2 rises to acquisition position, contacts simultaneously with sealing element 33
Make its compressive deformation, guarantee leakproofness, so that forming an airtight chamber with acquisition window 11 carries out light signal collection, collector
31 by the optical signal of acquisition upload to control panel 5 carry out quantitative analysis, this testing principle be it is existing, this structure improves inspection
The convenience of survey.
In the above whole process, temperature is controlled at 37 degree ± 0.3 degree by temperature control component 4, the structure of temperature control component 4 is simple
It is single, and this heating method has energy saving, temperature-controlled precision is high, in entire chamber the advantages of temperature uniformity, total
Micro-fluidic chip 7 is cooperated to realize the purge step of enzyme-catalyzed chemical luminescence with the mode of centrifugation in high-speed rotation with magnet ring 8
Suddenly, cooperation chip completes the processing of sample and the detection of enzyme-catalyzed chemical luminescence method, and lifting assembly 2 is cooperated to realize micro-fluidic core
The washing position of piece 7, reaction position, the free switching for acquiring position drive 7 high speed centrifugation of micro-fluidic chip, the mixing of positive and negative rotation liquid, essence
True position stops, and guarantees the even running of chip, compact-sized, easy to operate, reliable for operation, quickly detects, portable good,
High degree of automation improves working efficiency, ensure that the precision of detection.
One embodiment of the present invention has been described in detail above, but the content is only preferable implementation of the invention
Example, should not be considered as limiting the scope of the invention.It is all according to all the changes and improvements made by the present patent application range
Deng should still be within the scope of the patent of the present invention.
Claims (10)
1. the chemiluminescence immunoassay detection device based on micro-fluidic chip, it is characterised in that: including reaction chamber, be arranged described
In reaction chamber and by the micro-fluidic chip of rotating electric machine driving rotation；
Lifting assembly realizes micro-fluidic chip washing, reaction, the switching of acquisition position for driving the oscilaltion of rotating electric machine；
The lower end inside the reaction chamber is arranged in magnet ring, and absorption magnetic bead provides company when being used for micro-fluidic chip high-speed rotation
Continuous magnetic attraction；
Acquisition module, for acquiring optical signal and uploading to control panel；
Temperature control component meets enzyme reaction demand for controlling the temperature of reaction chamber；
Control panel controls the movement of acquisition module, temperature control component and rotating electric machine, controls the rotation of micro-fluidic chip and stop
Only.
2. the chemiluminescence immunoassay detection device according to claim 1 based on micro-fluidic chip, it is characterised in that: described
Lifting assembly is scissors lift platform structure, and the lifting assembly includes the horizontally disposed lifting platform in upper end, the rotating electric machine
It is installed on the lifting platform, the lifting assembly is driven by lifting motor, and the lifting motor controls its turn by control panel
It is dynamic.
3. the chemiluminescence immunoassay detection device according to claim 2 based on micro-fluidic chip, it is characterised in that: described
The structure of lifting assembly also packet pedestal, lifting platform described in the structure of the pedestal is identical and the two setting symmetrical above and below, the base
It is equipped between seat and the lifting platform and forms the first connecting rod, second connecting rod and shaft of cutting assemblies, under the cutting assemblies
End is slidably connected by sliding block and the pedestal, and there are lead screw, screw driving OQ t platform sliding in the upper end of the cutting assemblies
Connection, lead screw is driven by lifting motor to be rotated, and screw is slidably connected by being erected at the guide rod of lifting platform lower end with lifting platform.
4. the chemiluminescence immunoassay detection device according to claim 1 based on micro-fluidic chip, it is characterised in that: described
Acquisition module includes balancing stand, collector and sealing element, and the upper end of the reaction chamber is equipped with acquisition window, and the balance is set up
In the upper end of the sealed chamber, the sealing element is located at the lower end of the balancing stand, and the sealing element is flexible material, acquisition
Under state, the micro-fluidic chip, which is moved up, contacts setting with the sealing element, and the sealing element is located at the acquisition window
Outer ring, the collector is located at the upper end of the balancing stand and the corresponding acquisition window setting.
5. the chemiluminescence immunoassay detection device according to claim 4 based on micro-fluidic chip, it is characterised in that: described
The upper end of reaction chamber is set there are three acquisition window, and the lower end of balancing stand position corresponding with the acquisition window is equipped with
Sealing ring.
6. the chemiluminescence immunoassay detection device according to claim 1 based on micro-fluidic chip, it is characterised in that: described
Temperature control component includes the U-shaped air duct for being located at reaction chamber rear end and being connected to its inside, and the one end in the U-shaped air duct is as air inlet
Mouthful, the other end is equipped with heating component as air outlet, internal middle part, and the air inlet and air outlet are close to the reaction chamber
Place is equipped with aerofoil fan, and the temperature control component further includes being arranged in the indoor temperature sampler of reaction chamber, the temperature acquisition
Device is electrically connected with the control panel.
7. the chemiluminescence immunoassay detection device according to claim 1 based on micro-fluidic chip, it is characterised in that: described
Micro-fluidic chip includes dilution well, sample application hole, detection slot and waste liquid tank, and the dilution well and sample add
The connection of sample Kong Junyu mixing channel, the mixing channel are connected to detection slot, and the lower end of the detection slot is equipped with the cleaning communicated therewith
Liquid quantitative slot, the detection slot are connected to setting with waste liquid tank.
8. using the method for the chemiluminescence immunoassay detection device as claimed in claim 7 based on micro-fluidic chip, feature exists
In:
S1: device power-up prepares；
S2: addition detection sample to sample application hole adds dilution to the dilution well of micro-fluidic chip, will add
At micro-fluidic chip be put into reaction chamber and fixed with rotating electric machine；
S3: after being installed above, in reaction position after rotating electric machine and micro-fluidic chip decline, rotating electric machine drives micro-fluidic
Chip high speed rotation, cooperation micro-fluidic chip are sequentially completed the separation of sample and dilution, quantitative, dilution, heating；Make quantitatively complete
Sample and the mixed liquor of dilution enter the detection slot of micro-fluidic chip, the magnetic bead antibody freeze-drying pre-installed in detection slot is small
Ball, enzyme labelled antibody freeze-drying bead dissolve and react it, form double-antibody sandwich compound；
S4: lifting assembly decline makes micro-fluidic chip reach washing position, and magnetic bead is attached to chip inspection under the magneticaction of magnet ring
The bottom surface of slot, rotating electric machine high-speed rotation are surveyed, the liquid comprising extra enzyme labelled antibody enters waste liquid tank under the influence of centrifugal force,
And double-antibody sandwich compound is retained in detection slot under the action of magnetic attraction；
S5: lifting assembly rises, and so that magnetic bead is detached from the magnetic force constraint of magnet ring, and discharge the cleaning solution in cleaning solution quantitative slot, electricity
Machine rotation makes cleaning solution enter detection slot, and magnetic bead suspends again；
S6: rotating electric machine and micro-fluidic chip return to reaction position, discharge luminous substrate, rotation micro-fluidic chip make luminous substrate into
Enter detection slot, cooperation micro-fluidic chip completes the mixing of detection liquid, reaction；
S7: rotating electric machine drives micro-fluidic chip rotation, is aligned detection slot with acquisition module, the optical signal of acquisition is uploaded to
Control panel carries out quantitative analysis.
9. the application method of the chemiluminescence immunoassay detection device according to claim 8 based on micro-fluidic chip, special
Sign is: the temperature control component controlled at 37 degree ± 0.3 degree.
10. the application method of the chemiluminescence immunoassay detection device according to claim 8 based on micro-fluidic chip, special
Sign is: S5 and between repeat step 4,5,4, washed once with cleaning solution 2 times, thoroughly exclude unbonded enzyme labelled antibody, it is complete
At washing step.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910313140.7A CN109946460B (en) | 2019-04-18 | 2019-04-18 | Chemiluminescence immune detection device based on micro-fluidic chip and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910313140.7A CN109946460B (en) | 2019-04-18 | 2019-04-18 | Chemiluminescence immune detection device based on micro-fluidic chip and using method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109946460A true CN109946460A (en) | 2019-06-28 |
| CN109946460B CN109946460B (en) | 2023-09-22 |
Family
ID=67014544
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910313140.7A Active CN109946460B (en) | 2019-04-18 | 2019-04-18 | Chemiluminescence immune detection device based on micro-fluidic chip and using method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109946460B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346585A (en) * | 2019-07-10 | 2019-10-18 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory testing method and system |
| CN113009136A (en) * | 2020-08-21 | 2021-06-22 | 东莞东阳光医疗智能器件研发有限公司 | Small-sized multi-index detection sample analysis device |
| CN113324985A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Centrifugal micro-fluidic detection device and centrifugal micro-fluidic detection system |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070138450A1 (en) * | 2005-12-15 | 2007-06-21 | Bradley Norma | Mobile lift table with raisable casters |
| CN103376312A (en) * | 2012-04-24 | 2013-10-30 | 财团法人工业技术研究院 | Specimen immunoassay detection device |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
| CN207036852U (en) * | 2017-07-03 | 2018-02-23 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system |
| CN108970655A (en) * | 2018-06-04 | 2018-12-11 | 东莞东阳光科研发有限公司 | A kind of magnetic bead transfer device of micro-fluidic chip |
| CN109030813A (en) * | 2018-07-19 | 2018-12-18 | 东莞东阳光科研发有限公司 | A kind of chemiluminescence immunoassay detection micro-fluidic chip, detector and detection method |
| CN109046477A (en) * | 2018-07-23 | 2018-12-21 | 深圳市刚竹医疗科技有限公司 | Centrifugal microfluidic control device |
| CN208476767U (en) * | 2018-08-07 | 2019-02-05 | 李浩元 | Based on micro-fluidic biochemistry and immune integrated detection machine |
| CN109444440A (en) * | 2018-12-17 | 2019-03-08 | 北京博奥晶典生物技术有限公司 | A kind of centrifugal type microfludic detector with clamshell temperature control chamber |
| CN208607232U (en) * | 2018-07-19 | 2019-03-15 | 东莞东阳光科研发有限公司 | A kind of chemiluminescence immunoassay detection micro-fluidic chip and detector |
| CN209979648U (en) * | 2019-04-18 | 2020-01-21 | 天津诺迈科技有限公司 | Chemiluminescence immunodetection device based on micro-fluidic chip |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201924024U (en) * | 2010-12-13 | 2011-08-10 | 北京博晖创新光电技术股份有限公司 | Biological safety protecting device of micro-fluidic chip detection and diagnosis equipment |
| CN107942050B (en) * | 2017-11-10 | 2019-11-26 | 南京岚煜生物科技有限公司 | A kind of detection method of microfluidic chip based on magnetic bead technology |
| CN207786624U (en) * | 2017-11-27 | 2018-08-31 | 深圳华炎微测医疗科技有限公司 | Chemiluminescence testing microfluid control chip and chemiluminescence testing microfluid control chip system |
-
2019
- 2019-04-18 CN CN201910313140.7A patent/CN109946460B/en active Active
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070138450A1 (en) * | 2005-12-15 | 2007-06-21 | Bradley Norma | Mobile lift table with raisable casters |
| CN103376312A (en) * | 2012-04-24 | 2013-10-30 | 财团法人工业技术研究院 | Specimen immunoassay detection device |
| CN107102131A (en) * | 2017-07-03 | 2017-08-29 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system and its detection method |
| CN207036852U (en) * | 2017-07-03 | 2018-02-23 | 沈阳微流控生物科技有限公司 | A kind of full-automatic micro-fluidic chip fluorescence immunoassay detecting system |
| CN108970655A (en) * | 2018-06-04 | 2018-12-11 | 东莞东阳光科研发有限公司 | A kind of magnetic bead transfer device of micro-fluidic chip |
| CN109030813A (en) * | 2018-07-19 | 2018-12-18 | 东莞东阳光科研发有限公司 | A kind of chemiluminescence immunoassay detection micro-fluidic chip, detector and detection method |
| CN208607232U (en) * | 2018-07-19 | 2019-03-15 | 东莞东阳光科研发有限公司 | A kind of chemiluminescence immunoassay detection micro-fluidic chip and detector |
| CN109046477A (en) * | 2018-07-23 | 2018-12-21 | 深圳市刚竹医疗科技有限公司 | Centrifugal microfluidic control device |
| CN208476767U (en) * | 2018-08-07 | 2019-02-05 | 李浩元 | Based on micro-fluidic biochemistry and immune integrated detection machine |
| CN109444440A (en) * | 2018-12-17 | 2019-03-08 | 北京博奥晶典生物技术有限公司 | A kind of centrifugal type microfludic detector with clamshell temperature control chamber |
| CN209979648U (en) * | 2019-04-18 | 2020-01-21 | 天津诺迈科技有限公司 | Chemiluminescence immunodetection device based on micro-fluidic chip |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110346585A (en) * | 2019-07-10 | 2019-10-18 | 深圳金迈隆电子技术有限公司 | A kind of on piece laboratory testing method and system |
| CN113009136A (en) * | 2020-08-21 | 2021-06-22 | 东莞东阳光医疗智能器件研发有限公司 | Small-sized multi-index detection sample analysis device |
| CN113009136B (en) * | 2020-08-21 | 2024-04-05 | 东莞东阳光医疗智能器件研发有限公司 | Small multi-index detection sample analysis device |
| CN113324985A (en) * | 2021-06-16 | 2021-08-31 | 博奥生物集团有限公司 | Centrifugal micro-fluidic detection device and centrifugal micro-fluidic detection system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109946460B (en) | 2023-09-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109946460A (en) | Chemiluminescence immunoassay detection device and application method based on micro-fluidic chip | |
| CN106018784B (en) | Small electrical chemical illumination immunity analysis instrument and its analysis method | |
| CN102998473B (en) | Full-automatic chemiluminescence immune analyzer | |
| CN209727970U (en) | A kind of automatic cleaning incubates the detection device of mixing | |
| CN206339561U (en) | Small electrical chemical illumination immunity analysis instrument | |
| CN105628947B (en) | Whole blood automatic luminous immunoassay instant detector | |
| CN109999931A (en) | Chemiluminescence detection micro-fluidic chip and application method, reagent cleaning method | |
| CN102788780B (en) | Microfluidic chip for biological chemiluminescence detection and manufacturing method thereof | |
| JPH03135768A (en) | Analyzing method and automatic analyzer | |
| CN104297498A (en) | Fully automatic biochemical and luminescent immunoassay system | |
| CN102253228A (en) | Automatic chemiluminescence immunoassay device | |
| CN111796104A (en) | Exosome detection and typing microfluidic chip and exosome detection and typing method | |
| CN110883001A (en) | Magnetic bead cleaning device for analyzer | |
| CN109709349A (en) | A kind of chemiluminescence immunoassay system | |
| CN111077319A (en) | Micro-fluidic chip immunodetection kit and detection method thereof | |
| CN106199024A (en) | Automatic clinical chemistry analyzer and biochemical detection system | |
| CN208990746U (en) | Test solution agitating device and test solution stir suction means | |
| CN209979648U (en) | Chemiluminescence immunodetection device based on micro-fluidic chip | |
| WO2021068913A1 (en) | Magnetic particle luminescence micro-fluidic chip for multi-marker detection, and detection device | |
| WO2021068912A1 (en) | Magnetic particle luminescence micro-fluidic chip for multi-marker detection, and detection device | |
| CN113846009A (en) | Liquid separating extractor by magnetic bead method | |
| CN206557223U (en) | Full-automatic thrombelastogram instrument | |
| CN113125693B (en) | Small-sized portable full-automatic enzyme-linked immunoassay analyzer and application thereof | |
| CN109765391B (en) | Multi-index detection centrifugal test strip chip | |
| CN212770649U (en) | Centrifugal micro-fluidic chip integrating nucleic acid extraction and fluorescence amplification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |