CN109893642A - The Chinese medicine composition for treating grice diarrhoea - Google Patents
The Chinese medicine composition for treating grice diarrhoea Download PDFInfo
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Abstract
The present invention provides a kind of Chinese medicine composition, curative for effect for the treatment of grice diarrhoea using the special ratios Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae as active constituent, highly-safe.The invention also discloses the extracting methods of Chinese medicine composition active constituent, extracted, concentration, clear and bright, freeze-drying, granulation, whole grain, effectively reduce the tannin and oil-soluble impurities in Chinese medicine, significantly improve effective component in medicinal powder (pulchinenoside etc.) content, effective component is scattered in after carrier in high degree of dispersion state simultaneously, substantially increases the bioavailability of Chinese medicine composition.The present invention can effectively achieve the effect that stop dysentery, anti-inflammatory, anti-oxidant, treatment transmissible gastroenteritis of swine and popular diarrhoeal diseases, and curative for effect, highly-safe, suit large area to popularize application.
Description
Technical field
The invention belongs to veterinary drug technical fields, and in particular to a kind of Chinese medicine composition for treating grice diarrhoea.
Background technique
Grice diarrhoea is one of most commonly used epidemic disease in pig farm, and the disease incidence of diarrhea is up to 50.47% in clinic, sternly
The development of pig breeding industry is threatened again.The daily nutrition level of piglet, variation of ambient temperature, stress, bacterium and virus infection etc. be all
Cause grice diarrhoea principal element, wherein bacterium and virus infection are especially prominent, and wide-scale distribution can be carried out between swinery, accelerate disease
Disease is broken out.In winter, breaking out for the highly pathogenic disease such as transmissible gastroenteritis of swine and epidemic diarrhea is to cause grice diarrhoea
Principal element, which has the characteristics that route of transmission is more, speed is fast, susceptible animal group is wide, case fatality rate is high, after the onset of piglet
Can occur dehydration death in 1~2 day, the case fatality rate of 2~7 ages in days is up to 100%.It is not only caused in the pig raising in China huge
Economic loss, in Europe, North America, Asia etc., many countries are all in constantly report transmissible gastroenteritis of swine and epidemic diarrhea
The variation of cause of disease breaks out situation and its harm, is classified as among 117 kinds of deadly infectious diseases by OIE within 2018.For grice diarrhoea,
Especially piglet transmissible gastroenteritis and epidemic diarrhea, are presently mainly prevented and treated by vaccine inoculation, in the market
Be infectious the deadly infectious diseases such as gastroenteritis, epidemic diarrhea, rotavirus simply connected or multiple vaccines, but cause of disease is also continuous
It morphs, often will cause immuning failure, seriously endanger pig breeding industry development.
Summary of the invention
In order to solve the problems in the prior art, the purpose of the present invention is to provide a kind of Chinese medicine compositions, with specific ratio
The example Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae are active constituent, for the treatment of grice diarrhoea, curative effect
Definitely, highly-safe.Unless otherwise specified, percentage of the present invention is weight percentage.
The object of the present invention is achieved like this:
A kind of Chinese medicine composition, it includes active constituent and/or pharmaceutically acceptable carrier, the active constituent contains
There are following bulk pharmaceutical chemicals: the Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae.
Further, Chinese medicine composition as described above, the active constituent are made of the raw material of following mass ratioes: hoary hair
30~90 parts of father-in-law, 20~80 parts of radix bupleuri, 10~60 parts of the tuber of pinellia, 10~60 parts of Rhizoma Atractylodis Macrocephalae, 10~60 parts of Poria cocos, 10~60 parts of rhizoma zingiberis,
5~30 parts of campanulaceae, 5~30 parts of Radix Glycyrrhizae.
Further, Chinese medicine composition as described above, the active constituent are made of the raw material of following mass ratioes: hoary hair
30~60 parts of father-in-law, 20~50 parts of radix bupleuri, 10~40 parts of the tuber of pinellia, 10~40 parts of Rhizoma Atractylodis Macrocephalae, 10~40 parts of Poria cocos, 10~40 parts of rhizoma zingiberis,
5~20 parts of campanulaceae, 5~20 parts of Radix Glycyrrhizae.
The extracting method of active constituent in above-mentioned Chinese medicine composition, comprising the following steps: extract Chinese medicine composition；
Extracting solution is into concentration；Extract after concentration is filtered removing insoluble impurities；Medical fluid is clear and bright after filtering；Medical fluid extraction after clear and bright
It takes, medical fluid is concentrated under reduced pressure after extraction, dry and granulation.
Above-mentioned Chinese medicine composition extract step be weigh in parts by mass the Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos,
Rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae are added 50% alcohol of mass percentage concentration and impregnate, and refluxing extraction filters to obtain medical fluid.Preferably, Chinese medicine group
Close object and 50% alcohol solid-liquid ratio 1:8~15 (volume ratio), 0.5~1h of soaking time, 0.5~1.5h of reflowing time, mistake
Filter out insoluble impurities.
The above-mentioned clear and bright step of medical fluid is the chitosan solution for being 1% by the medical fluid addition content after concentration, stands, crosses and filter out
Decontamination.Preferably, by the medical fluid after concentration at 40~60 DEG C, adjust pH be 5.0~6.0, addition mass percent be 8~
12% 1% chitosan solution of content stands 12~24 hours, and filtering, filtrate is concentrated into 0.5~1.0 times of crude drug amount, adds
The petroleum ether that 0.8~3.5 times of medicine liquid volume, mixes well, and stands 3~6 hours, and aqueous is collected in extraction.
Specifically, the traditional chinese medicine composition of the invention, which is characterized in that the extracting method of active constituent the following steps are included:
A, the Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae are weighed in parts by mass, by solid-liquid ratio 1:8
~15 add 50% alcohol, impregnate 0.5~1.0h, 80 DEG C of 0.5~1.5h of refluxing extraction, and filtering obtains medical fluid 1；
B, the dregs of a decoction are filtered by solid-liquid ratio 1:8~15 plus water, 0.5~1.5h of refluxing extraction, are obtained medical fluid 2 after step a；
C, the dregs of a decoction are filtered by solid-liquid ratio 1:8~15 plus water, 0.5~1.5h of refluxing extraction, are obtained medical fluid 3 after step b；
D, medical filtration obtained by collection step a, b, c merges, and recovered alcohol, 80 DEG C are concentrated into crude drug amount: medical fluid 1:3
~6,40 DEG C~60 DEG C are cooled to, adjusting pH is 5.0~6.0, and the chitosan solution that 8%~12% content of addition is 1% is stood
24 hours, filtering, 80 DEG C of filtrate were concentrated into the 0.5~1.0 of crude drug amount volume, and the petroleum ether of 0.8~3.5 times of addition is sufficiently mixed
It is even, 3~6 hours are stood, aqueous is collected in extraction.
E, medical fluid obtained by step d adds n-butanol, and sufficiently after extraction, 50~70 DEG C of reduced-pressure backflows recycle n-butanol, collects
Medical fluid is filtered under diminished pressure, and collects filtrate, is freeze-dried into powder.
F, 80 mesh are crossed in the extract obtained crushing of step e, both obtain medicinal powder.
The traditional chinese medicine composition of the invention, pharmaceutically acceptable carrier include amylum pregelatinisatum, cornstarch, microcrystalline cellulose
Element, mannitol, dried starch, sodium carboxymethyl starch, low-substituted hydroxypropyl cellulose, croscarmellose sodium, the poly- second of crosslinking
The mixing of one or more of alkene pyrrolidone, magnesium stearate, superfine silica gel powder, talcum powder.
The traditional chinese medicine composition of the invention, pharmaceutically acceptable carrier further include corrigent, selected from Sucralose, xylitol,
The mixing of one or more of acesulfame potassium, Aspartame, essence.
Specifically, the traditional chinese medicine composition of the invention include 15~20 parts of medicinal powder, 60~80 portions of soluble starches and 0.5~
1.5 parts of corrigents.Starch slurry is made in medicinal powder addition soluble starch, 150mL/min whitewashes, and 100 DEG C carry out boiling system
Grain and drying arrange particle and cross No. 1 sieve, but No. 5 are sieved, both.
The traditional chinese medicine composition of the invention is for preventing or treating the application in grice diarrhoea drug.Further, the diarrhea is
Transmissible gastroenteritis or/and epidemic diarrhea.
Beneficial effect
The present invention provides a kind of Chinese medicine composition, with the special ratios Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, tangerine
Stalk, Radix Glycyrrhizae are active constituent, curative for effect for the treatment of grice diarrhoea, highly-safe.The present invention also provides Chinese traditional medicine compositions
The extracting method of object active constituent, extracted, concentration, it is clear and bright, freeze-drying, granulation, whole grain, effectively reduce
Tannin and oil-soluble impurities in medicine, hence it is evident that improve effective component in medicinal powder (pulchinenoside etc.) content, while effectively at
It is scattered in after carrier in high degree of dispersion state, substantially increases the bioavailability of Chinese medicine composition.Chinese medicine group of the present invention
Closing object, to lead to diarrhea piglet stop dysentery effect to folium sennae obvious, and 2-6h loose stool rate is extremely significant to be lower than positive controls (P < 0.01).This hair
Bright Chinese medicine composition causes scorching piglet antiphlogistic effects good lipopolysaccharides, can be substantially reduced NO, IL-1 in macrophage, TNF-α
Content.The traditional chinese medicine composition of the invention can significantly improve CAT, T-SOD, GSH-Px vigor, while reduce MDA content, to improve
The activity of the anti-oxidant relevant enzyme of piglet.The present invention can significantly reduce the death rate of PEDV and TGEV infection piglet in clinic, effectively
It treats PEDV and TGEV and infects piglet, up to 72.09%, infection TGEV piglet effective percentage reaches infection PEDV piglet effective percentage
80.65%, even the piglet of PEDV and TGEV mixed infection, the traditional chinese medicine composition of the invention effective percentage is still up to 60.61%.
In short, the traditional chinese medicine composition of the invention particle can effectively reach stop dysentery, anti-inflammatory, adjusting gastrointestinal peristalsis, treatment pig transmissible stomach and intestine
The effect of scorching and popular diarrhoeal diseases, curative for effect, highly-safe, suit large area to popularize application.
Detailed description of the invention
Fig. 1 is the survival analysis result figure for treating piglet.
Specific embodiment
1 traditional Chinese medicine particle Study on Preparation of embodiment
(1) Chinese bulbul 3kg, radix bupleuri 2kg, tuber of pinellia 1kg, Rhizoma Atractylodis Macrocephalae 1kg, Poria cocos 1kg, rhizoma zingiberis 1kg, campanulaceae 0.5kg, sweet is weighed
Careless 0.5kg adds 50% ethanol solution 100L, using being steam heated to 70~75 DEG C, after impregnating 1.0h, and 80 DEG C of refluxing extractions
1.0h is separated by filtration medical fluid and the dregs of a decoction.
(2) gained dregs of a decoction addition 100L water is filtered according to step (1) the method to carry out boiling extraction, filter, receive respectively
Collect medical fluid.
(3) gained dregs of a decoction addition 100L water is filtered according to step (1) the method to carry out boiling extraction, filter, receive respectively
Collect medical fluid.
(4) medical fluid obtained by step (1), step (2), step (3) is merged, is concentrated by steam pressure-reducing, thickening temperature is
75 DEG C, 5 times of volumes to be concentrated to crude drug amount stop concentration.
(5) 60 DEG C are cooled to step (4) medical fluid, adjusting pH is 5.5, the chitosan solution that 10% content of addition is 1%,
24 hours are stood, filtering, 70~75 DEG C of 0.5~1.0 times of volumes for being concentrated under reduced pressure into crude drug amount.Medical fluid is cooled to room temperature, addition 2
Petroleum ether again, mixes well, and stands 4 hours, and extraction removes oil-soluble impurities.
(6) step (5) medical fluid is added into n-butanol, sufficiently after extraction, 60 DEG C of reduced-pressure backflows recycle n-butanol, collect medicine
Liquid.
(7) step (6) medical fluid is freeze-dried.
(8) step (7) dry drug is crushed, crosses 80 mesh.
(9) by medicinal powder obtained by step (8) after adding auxiliary material, one-step method granulation is carried out, dry, whole grain, both.
Embodiment 2
Referring to embodiment 1 extraction process, investigate extraction conditions (such as soaking time, solid-liquid ratio, decocting time) with it is clear
Influence of the bright condition (such as chitosan additive amount, fluid temperature, medical fluid pH) to extraction effect.(1) investigation of extraction conditions: white
Head father-in-law is the main ingredient in Chinese medicine composition, and pulchinenoside is its main labeled ingredient, therefore with the content of pulchinenoside
For inspection target, by soaking time (0,0.5,1.0 hour), solid-liquid ratio (1:15,1:10,1:8), decocting time (1.5,
1.0,0.5 hour) etc. factors, investigate extracting method.
1 extracting method of table is investigated
The results show that the content of pulchinenoside is 2.35%~5.77% in extracting solution, B (solid-liquid ratio) > C in table 1
(decocting time) > A (soaking time), and B3 > B1 > B2, C2 > C1 > C3, A3 > A1 > A2.Determine that extraction process is
A3B3C2, i.e. immersion 1h, each solid-liquid ratio is respectively 1:10,1:10,1:8, decocts 1h every time.
The assay result of 2 pulchinenoside of table
(2) investigation of clear and bright condition: using 1% chitosan solution as clear and bright dose, the weight of precipitating is main performance assessment criteria,
With chitosan additive amount (8%, 10%, 12%), fluid temperature (40 DEG C, 50 DEG C, 60 DEG C), medical fluid pH (5.0,5.5,6.0) etc.
Factor investigates clear and bright condition.
Table 3 is clarified condition and is investigated
The results show that 14.13%~18.73% that Sediment weight is medical fluid is separated in clear and bright liquid, B (medical fluid temperature in table 3
Degree) > C (medical fluid pH) > A (additive amount of chitosan), and B3 > B2 > B1, C1 > C2 > C3, A2 > A3 > A1, variance analysis
The results show that difference is not significant (< 0.05) between three factors.Accordingly, it is determined that clear and bright condition is A2B3C1, i.e. fluid temperature
Be 60 DEG C, when pH5.0, add 10% chitosan solution carry out it is clear and bright.
The clear and bright test result of table 4
Embodiment 3, traditional Chinese medicine particle lead to diarrhea the stop dysentery effect disquisition of piglet to folium sennae
According to embodiment 1 as a result, implementation is carried out as follows.
By 50 28~35 age in days piglets, it is divided into blank group according to the random district's groups of weight, positive controls, high, medium and low dose
Amount group, every group 10, half male and half female.Blank group and positive controls stomach-filling physiological saline, high, medium and low dosage group distinguish stomach-filling
The traditional Chinese medicine particle of 2.0g/kg, 1.0g/kg, 0.5g/kg, after last dose 30 minutes, other each groups are filled in addition to blank group
Stomach folium sennae, the single only raising of piglet is in the total just several and muck number on metabolic cage, observing and recording piglet, statistics 2h, 4h, 6h
Diarrhea rate and loose stool rate.Diarrhea rate=diarrhea number of animals/this group of animal number × 100%；Loose stool rate=every animal institute
The muck number of row/always just count × 100%.
Antidiarrheal result see the table below 5.
5 antidiarrheal result of table
The results show that blank group piglet does not occur diarrhea situation during the whole test process.In 2h, high, middle dose group son
There is not diarrhea in pig, and loose stool rate is extremely significant to be lower than positive controls and low dose group (P < 0.01), and low dose group is extremely significant low
In positive controls (P < 0.01)；After 4h, positive controls diarrhea rate reaches 100%, and high dose group and middle dose group are relatively
Low, loose stool rate is extremely significant to be lower than positive controls and low dose group (P < 0.01), and low dose group is extremely significant to be lower than positive controls
(P < 0.01)；After 6h, positive controls and low dose group grice diarrhoea rate reach 100%, and high dose group and middle dose group are distinguished
Reach 70% and 60%, loose stool rate is extremely significant to be lower than positive controls and low dose group (P < 0.01).
Embodiment 4, traditional Chinese medicine particle cause the antiphlogistic effects of scorching piglet macrophage to study lipopolysaccharides
According to embodiment 1 as a result, implementation is carried out as follows.
After selecting 28 age in days piglets, intraperitoneal injection to remain hydroxy acid sodium 4 days, piglet macrophage is extracted.Even if will be thin
Born of the same parents are seeded on 96 plates, are randomly divided into blank group, lipopolysaccharide group, and high, medium and low dosage group adds 50 μ g/L's in addition to blank group
LPS 48 hours after culture, detects the content of NO, IL-1 in cell liquid, TNF-α.
Anti-inflammatory result see the table below 6.
The anti-inflammatory result of table 6
The results show that NO, IL-1, TNF-α content increase in macrophage after addition lipopolysaccharides.Traditional Chinese medicine particle
NO, IL-1 in macrophage, TNF-α content can be reduced, wherein high, medium and low dosage group NO content is extremely significant to be lower than lipopolysaccharides
Group (P < 0.01)；High, middle dose group IL-1 content is substantially less than lipopolysaccharide group (P < 0.05)；Wherein high, medium and low dosage group
TNF-α content is substantially less than lipopolysaccharide group (P < 0.05).
Embodiment 5, traditional Chinese medicine particle cause the antioxidant effect of oxidation piglet to study lipopolysaccharides
According to embodiment 1 as a result, implementation is carried out as follows.
By 50 28~35 age in days piglets, it is divided into blank group, lipopolysaccharide group, high, medium and low dosage according to the random district's groups of weight
Group, every group 10, half male and half female.Blank group and lipopolysaccharides stomach-filling physiological saline, high, medium and low dosage group distinguish stomach-filling 2.0g/
The traditional Chinese medicine particle of kg, 1.0g/kg, 0.5g/kg, after last dose 3 hours, in addition to blank group, each test group piglet abdomen
100 μ g/kg lipopolysaccharides are injected in film, 3 hours acquisition blood after injection separates serum, measures CAT, T-SOD, GSH-Px, MDA
Content.
Anti-oxidant result see the table below 7.
The anti-oxidant result of table 7
The results show that the vigor of the anti-oxidant relevant enzyme of piglet reduces after injection lipopolysaccharides, hence it is evident that reduce the anti-of piglet
Oxidability.Traditional Chinese medicine particle can effectively improve CAT vigor, and middle dose group is significantly higher than lipopolysaccharide group and low dose group
(P < 0.05)；T-SOD vigor is improved, high dose group and middle dose group are significantly higher than lipopolysaccharide group and low dose group (P <
0.05)；GSH-Px vigor is improved, high dose group and middle dose group are extremely significant higher than lipopolysaccharide group (P < 0.01), are significantly higher than low
Dosage group (P < 0.05)；MDA content is reduced, high dose group and middle dose group are extremely significant lower than lipopolysaccharide group (P < 0.01), show
It writes and is lower than low dose group (P < 0.05).
The anti-TGEV and PEDV effect disquisition of embodiment 6, traditional Chinese medicine particle
According to embodiment 1 as a result, implementation is carried out as follows.
The host cell of test basis TGEV and PEDV is different, selects ST cell and Vero cell as host cell, divides
Cell suspension is not diluted to 1 × 105A/ml accesses 96 porocyte culture plates, and after covering with single layer, blank group, disease is respectively set
Poison group and 3 test groups, each group of 8 holes, blank group only add maintaining liquid as control, and virus group virus infection tests 1 group
The drug and the mixture after virus function 2 hours that cell adds maximal non-toxic concentration, act on 48 hours after addition；Test 2 groups
Virus infection again after cell first adds drug effect 48 hours of maximal non-toxic concentration；3 groups of cell elder generation virus infections are tested to add again
Add the drug effect of maximal non-toxic concentration 48 hours, after the test, using the light absorption value (OD value) of mtt assay detection cell, meter
Calculate cellular damage inhibiting rate.Damage inhibiting rate=(test group OD value-virus organizes OD value)/(blank control group OD value-virus group OD
Value) × 100%.
Antiviral result see the table below 8.
The anti-TGEV and PEDV result of table 8
The results show that the vigor of cell is substantially reduced after being inoculated with TGEV and PEDV, OD value is extremely significant to be lower than blank group (P
< 0.01)；After being inoculated with TGEV, 2 groups of cellular damage inhibiting rates of test are extremely significant to be higher than 3 groups (P < 0.01) of 1 group of test and test,
Illustrate that traditional Chinese medicine particle mainly aims at prevention during anti-TGEV；After being inoculated with PEDV, 3 groups of 2 groups of test and test
Cellular damage inhibiting rate it is extremely significant be higher than test 1 group (P < 0.01), illustrate traditional Chinese medicine particle be during anti-PEDV with
Based on prevention and treatment.
Embodiment 7, traditional Chinese medicine particle treatment transmissible gastroenteritis of swine and popular diarrhoeal diseases
According to embodiment 1 as a result, implementation is carried out as follows.
Piglet 150 of winter diarrhea are selected from clinic, acquire excrement respectively, quickly detect PEDV and TGEV, detection
Traditional Chinese medicine particle is used to treat it immediately for positive, every piglet is administered according to the dosage of 2.0g/kg,
2 times a day, successive administration 7 days are observed continuously 2 weeks after administration, judge the treatment condition of piglet.It is sticked into through PEDV and TGEV detection
Row detects, and 43 are the PEDV positive in 150 diarrhea piglets, and 31 are positive for TGEV, and 66 are PEDV and TGEV mixed infection,
Concrete condition such as Fig. 1.By the survival analysis of piglet, main vital is concentrated after infection in 1 week, and Chinese medicine composition is used continuously
After particle is treated, the death of piglet is significantly controlled, and the death rate reduces, and after being observed continuously 2 weeks, PEDV is positive young
Pig effective percentage reaches 72.09%, TGEV and reaches 80.65% for positive piglet effective percentage, and the piglet effective percentage of mixed infection reaches
60.61%, illustrate that traditional Chinese medicine particle can effectively treat PEDV and TGEV infection piglet.
Several preferred embodiments of the invention have shown and described in above description, but as previously described, it should be understood that the present invention
Be not limited to forms disclosed herein, should not be regarded as an exclusion of other examples, and can be used for various other combinations,
Modification and environment, and the above teachings or related fields of technology or knowledge can be passed through within that scope of the inventive concept describe herein
It is modified.And changes and modifications made by those skilled in the art do not depart from the spirit and scope of the present invention, then it all should be in this hair
In the protection scope of bright appended claims.
Claims (10)
1. a kind of Chinese medicine composition, it includes active constituent and/or pharmaceutically acceptable carrier, the active constituent is by following
The raw material of mass ratio forms: 30 ~ 90 parts of the Chinese bulbul, 20 ~ 80 parts of radix bupleuri, 10 ~ 60 parts of the tuber of pinellia, 10 ~ 60 parts of Rhizoma Atractylodis Macrocephalae, Poria cocos 10 ~ 60
Part, 10 ~ 60 parts of rhizoma zingiberis, 5 ~ 30 parts of campanulaceae, 5 ~ 30 parts of Radix Glycyrrhizae.
2. Chinese medicine composition as described in claim 1, it is characterised in that: the Chinese medicine composition, the active constituent is under
The raw material for stating mass ratio forms: 30 ~ 60 parts of the Chinese bulbul, 20 ~ 50 parts of radix bupleuri, 10 ~ 40 parts of the tuber of pinellia, 10 ~ 40 parts of Rhizoma Atractylodis Macrocephalae, Poria cocos 10 ~
40 parts, 10 ~ 40 parts of rhizoma zingiberis, 5 ~ 20 parts of campanulaceae, 5 ~ 20 parts of Radix Glycyrrhizae.
3. Chinese medicine composition as claimed in claim 1 or 2, it is characterised in that: active constituent mentions in the Chinese medicine composition
Taking step is to weigh the Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae in parts by mass, and quality percentage is added
50% alcohol of concentration impregnates, and refluxing extraction filters to obtain medical fluid；Medical fluid is concentrated, then the medical fluid addition after concentration will be contained
The chitosan solution that amount is 1% stands and carries out clear and bright, filtering, extraction, dry and granulation.
4. Chinese medicine composition as claimed in claim 3, it is characterised in that: the Chinese medicine composition medicinal material and 50% alcohol feed liquid
Than 1:8 ~ 15(volume ratio), 0.5 ~ 1h of soaking time, 0.5 ~ 1.5h of reflowing time.
5. Chinese medicine composition as described in claim 3 or 4, it is characterised in that: by the medical fluid after concentration at 40 ~ 60 DEG C, adjust
PH is 5.0 ~ 6.0,1% chitosan solution of content that addition mass percent is 8 ~ 12%, stands and carries out clear and bright, filtering, and filtrate is dense
It is reduced to 0.5 ~ 1.0 times of crude drug amount, the petroleum ether of 0.8 ~ 3.5 times of medicine liquid volume of addition mixes well, and stands 3 ~ 6 hours, extraction
It takes, collects aqueous.
6. Chinese medicine composition as claimed in claim 3, it is characterised in that: the Chinese medicine composition, which is characterized in that activity at
Point extraction the following steps are included:
A, the Chinese bulbul, radix bupleuri, the tuber of pinellia, Rhizoma Atractylodis Macrocephalae, Poria cocos, rhizoma zingiberis, campanulaceae, Radix Glycyrrhizae are weighed in parts by mass, by solid-liquid ratio 1:8 ~ 15
Add 50% alcohol, impregnate 0.5 ~ 1.0 h, 80 DEG C of 0.5 ~ 1.5 h of refluxing extraction, filtering obtains medical fluid 1；
B, the dregs of a decoction are filtered by solid-liquid ratio 1:8 ~ 15 plus water, 0.5 ~ 1.5 h of refluxing extraction, are obtained medical fluid 2 after step a；
C, the dregs of a decoction are filtered by solid-liquid ratio 1:8 ~ 15 plus water, 0.5 ~ 1.5 h of refluxing extraction, are obtained medical fluid 3 after step b；
D, medical filtration obtained by collection step a, b, c merges, and recovered alcohol, 80 DEG C are concentrated into crude drug amount: medical fluid is 1:3 ~ 6, cold
But to 60 DEG C, adjusting pH is 5.0 ~ 6.0, and the chitosan solution that 10% content of addition is 1% stands 24 hours, filtering, 80 DEG C of medical fluid
It is concentrated into the 0.5 ~ 1.0 of crude drug amount, the petroleum ether of 0.8 ~ 3.5 times of addition mixes well, and stands 3 ~ 6 hours, and extraction removes liposoluble
Property impurity, collect aqueous；
E, medical fluid obtained by step d adds n-butanol, and sufficiently after extraction, 50 ~ 70 DEG C of reduced-pressure backflows recycle n-butanol, collects medical fluid,
It is filtered under diminished pressure, collects filtrate, be freeze-dried into powder；
F, 80 mesh are crossed in the extract obtained crushing of step e, both obtain medicinal powder.
7. Chinese medicine composition as claimed in any one of claims 1 to 6, it is characterised in that: the pharmaceutically acceptable carrier packet
Include amylum pregelatinisatum, cornstarch, microcrystalline cellulose, mannitol, dried starch, sodium carboxymethyl starch, low substituted hydroxy-propyl fiber
One of element, croscarmellose sodium, crosslinked polyvinylpyrrolidone, magnesium stearate, superfine silica gel powder, talcum powder are several
Kind mixing.
8. Chinese medicine composition as claimed in claim 7, it is characterised in that: the pharmaceutically acceptable carrier further includes flavoring
Agent is mixed selected from one or more of Sucralose, xylitol, acesulfame potassium, Aspartame, essence.
9. such as the described in any item Chinese medicine compositions of claim 1-8, it is characterised in that: the Chinese medicine composition includes 15-20
Part medicinal powder, 60 ~ 80 portions of soluble starches and 0.5 ~ 1.5 part of corrigent；Medicinal powder addition soluble starch is made starch slurry, 150
ML/min whitewashes, 100 DEG C of progress boiling granulatings and drying, arranges particle and crosses No. 1 sieve, but No. 5 sieves, addition corrigent are mixed
It is even, both.
10. Chinese medicine composition as claimed in claims 1-9, it is characterised in that: the Chinese medicine composition is for preventing or treating
Application in grice diarrhoea drug, preferably transmissible gastroenteritis or/and epidemic diarrhea.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910304136.4A CN109893642A (en) | 2019-04-16 | 2019-04-16 | The Chinese medicine composition for treating grice diarrhoea |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201910304136.4A CN109893642A (en) | 2019-04-16 | 2019-04-16 | The Chinese medicine composition for treating grice diarrhoea |
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| CN107595954A (en) * | 2017-10-10 | 2018-01-19 | 上海中医药大学附属曙光医院 | A kind of Chinese medicine composition for reducing body fat and treating Central obesity and its application |
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| CN106491834A (en) * | 2016-12-09 | 2017-03-15 | 重庆市畜牧科学院 | A kind of veterinary Chinese medicinal composition and its preparation method and application of granule |
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Application publication date: 20190618 |