CN109874750B

CN109874750B - Artificial breeding method of Cereus

Info

Publication number: CN109874750B
Application number: CN201910221589.0A
Authority: CN
Inventors: 展茂魁; 陈华; 赵斌; 司徒春南; 路纪芳; 方维业; 杨朝应; 杜文先; 孙亚茹; 王淑君
Original assignee: ZUNYI INSTITUTE OF FORESTRY
Current assignee: ZUNYI INSTITUTE OF FORESTRY
Priority date: 2019-03-22
Filing date: 2019-03-22
Publication date: 2021-09-03
Anticipated expiration: 2039-03-22
Also published as: CN109874750A

Abstract

The invention discloses an artificial breeding method of Cereus, which selects larvae or pupae of bees or wasps as a substitute host of Cereus went from Cereus went, screens out proper breeding conditions, and successfully realizes artificial large-scale breeding of Cereus went from Cereus went.

Description

Artificial breeding method of Cereus

Technical Field

The invention relates to the technical field of biological pest control, in particular to an artificial breeding method of ceratophaga xylostella.

Background

The Neodiprions huizeensis of Hymenoptera of Memenoptera of Meadoptera of Diprionidae of Onagraceae is a leaf-eating pest with serious harm to Pinus armandii and Pinus yunnanensis. Two pinus huashanensis, which are mainly distributed in the great county of binu, Weining and Hutch in Guizhou, are distributed intensively and have a large area. The Huizhou pine needle wasp occurs for 2 generations in 1 year in Guizhou, and pupae are mainly cocooned and overwinter on a soil layer, a stone block and a dead branch and fallen leaf layer around a crown, and are often damaged by gathering larva on the tips of pine branches in groups, so that the whole branches can be eaten in a short time, and all pine needles can be eaten when the damage is serious. The pinus armandi does not have the characteristics of secondary germination of pinus armandi, tip drawing and the like, and has no leaf sheath, so that the whole pinus armandi is withered when more than 90% of pinus armandi is eaten.

We' group, in cooperation with the expert of the forest environmental protection agency of Chinese forest academy of sciences, found 1 important parasitic natural enemy insect in the pupa of the Meadowrue maidenhair, which harms Pinus armandii: hedera helvetica (Piliferocallis henghangensis Yang et Zhan, sp.n.). The length of the bee body is 1.5-2.0mm, the whole body is dark green and has golden metallic luster, but the back of the abdomen is dark purple, and the compound eye is dark purple; the tentacle handle is yellow, the peduncle is light brown, and the cord and rod are dark brown; each foot base joint is yellow-white, the trochanter and the knee joint are white, and the rest parts of the foot are light brown; the wings are transparent, the veins of the front wings are light brown, and the front wings have 4 tobacco spots; 1 below the anterior border, 1 below the nevus, and 2 below and outside the large smoke spots below the renal nevus. Through long-term research, the biological characteristics of the Hedysosma longituba Smith are deeply understood, and an artificial breeding method is explored on the basis, so that the prevention and control of the Meadowrue Hudysosma longituba Smith by adopting the Hedysosma longituba Smith can be possible.

Disclosure of Invention

The invention aims to provide an artificial breeding method of ceratophaga, which realizes that the ceratophaga hutchinsonii var mairei (Piliferocallis heizhansensis Yang et Zhan, sp.n.) is used for preventing and treating the sphagnus evansi.

In order to achieve the purpose, the invention provides the following technical scheme:

an artificial breeding method of Cereus, which is characterized by comprising the following steps:

(1) cleaning the finger-shaped tube, sterilizing and drying in the air;

(2) selecting larvae or pupae of bees or wasps as a substitute host, and placing the substitute host into the middle lower part or the bottom of the finger-shaped tube;

(3) inoculating fertilized female wasps of the Cerasifera into the finger-shaped tube, wherein the number ratio of the Cerasifera to the alternative hosts is 1:1-1:5, plugging the open end of the finger-shaped tube with a degreasing cotton after the bee inoculation is finished, and culturing the finger-shaped tube in a climatic chamber;

the small hornet is a Hedgersia wasp with calluses and a little hornet;

preferably, the larvae are capped larvae; preferably, the pupae are milk white pupae;

preferably, the number ratio of the female wasps of the small hornet to the alternative hosts is 1:2-1: 3;

preferably, the culture conditions in the climatic chamber are: the relative humidity is 30-70%, the temperature is 15-32 ℃, and the light period is L8-16: D16-8; more preferably, the culture conditions in the climatic chamber are: the relative humidity is 40-60%, the temperature is 24-26 ℃, and the photoperiod is L10: D14.

The invention also provides application of the Cereus went in controlling the New Zealand wasps, and the Cereus went cultured by the invention is subpackaged by finger tubes and delivered to a forest for release.

The invention has the beneficial effects that:

1. the larvae or pupae of the bees or wasps as the alternative hosts are easy to obtain, the breeding technology of the alternative hosts is mature, the breeding cost is low, the artificial breeding cost of the Cereus went from the Cereus went is obviously reduced, and the large-scale application of the Cereus went from the Cereus went becomes possible.

2. The larva or pupa of the bee or the wasp is used as a substitute host to breed the Ceriporia manspica Hemsl, the breeding efficiency is high, and the Ceriporia manspica Hemsl Semsl obtained by replacing the host with a single head is higher than that of a natural host (the Ceriporia manspica).

3. The parasitism effect of the Heracleum Chapteri Seedler and the Ceratopteris cunea bred by adopting the alternative host on the New Zephysalis exsiccatus is excellent.

Drawings

The invention is further explained below with reference to the figures and examples;

FIG. 1 is a diagram showing the state of Cereus flavus wasp bred by using Chinese bee larva;

Detailed Description

The technical solution of the embodiment of the present invention is clearly and completely described as follows:

example 1

1. The test method comprises the following steps:

(1) cleaning the finger-shaped tube, sterilizing and drying in the air;

(2) using larvae of Chinese bees as alternative hosts, and placing the alternative hosts at the bottom of the finger-shaped tube;

(3) inoculating fertilized hermaphrodite wasp hornet trichogramma female bees into the finger-shaped tube, wherein the number ratio of the hermaphrodite wasp hornet female bees to the alternative host is 1:1, plugging the open end of the finger-shaped tube with a degreased cotton after the bee inoculation is finished, and putting the finger-shaped tube into a climatic chamber for culturing for 22-28 days until the hermaphrodite wasp hornet trichogramma is completely emerged; the culture conditions in the artificial climate box are as follows: relative humidity of 50%, temperature of 25 deg.C, light period L12: D12. Observing the oviposition, hatching, larva and eclosion conditions of the Cereus flavus every day until all the Cereus flavus is eclosion, and counting the number of adult bees;

(4) setting a contrast, replacing the larva of the Chinese bee with the cocoon of the Beehive newly-leaved bee, the tussah pupa and the coccid of the pink bollworm respectively, and then repeating the steps (1), (2) and (3) respectively to obtain a contrast 1, a contrast 2 and a contrast 3. The above experimental treatments were all repeated 3 times.

TABLE 1 Effect of different alternative hosts on breeding Oak wasp callorum

Treatment of	Average bee number of single host
		The invention	52
Control 1	12
		Control 2	-
Control 3	-

As can be seen from table 1, the larva of the bee is used as the alternative host to breed the erethistle wasp, the number of the single host to breed the wasp horneri is higher than that of the natural host (the huozhong maiden pine leaf bee), and in addition, the tussah pupa and the red bollworm cocoon can not be used as the alternative host to breed the erethistle wasp horneri.

Example 2

1. The test method comprises the following steps:

(1) cleaning the finger-shaped tube, sterilizing and drying in the air;

(2) taking capped larvae of the Vespa mandarinia as a substitute host, and putting the substitute host at the bottom of the finger-shaped tube;

(3) inoculating fertilized hermaphrodite wasp hornet trichoderma brevifiliformis into the finger-shaped tube, wherein the number ratio of the hermaphrodite wasp hornet to the alternative host is 1:2, plugging the open end of the finger-shaped tube with a degreased cotton after the bee inoculation is finished, and culturing the finger-shaped tube in a climatic chamber for 22-28 days until all the hermaphrodite wasp hornet is eclosized; the culture conditions in the artificial climate box are as follows: relative humidity 60%, temperature 25 deg.C, light period L10: D14. Observing the oviposition, hatching, larva and eclosion conditions of the Cereus flavus every day until all the Cereus flavus is eclosion, and counting the number of adult bees;

(4) setting a comparison, replacing capped larvae of the Vespa mandarinia with cocoons of the New Zea mairei, and repeating the steps (1), (2) and (3) respectively to obtain a comparison A;

(5) selecting female hermaphrodite wasps obtained by breeding different hosts, respectively inoculating the female hermaphrodite wasps to an insect breeding cage filled with the Meristotheca hutchuensis cocoons, wherein the ratio of the female hermaphrodite wasps to the Meristotheca hutchuensis cocoons is 1:200, and then placing the insect breeding cage into a phytotron to be cultured for 22-28 days until the all the Meristotheca hutchuensis wasps are eclosized; the culture conditions in the artificial climate chamber are as follows: relative humidity 60%, temperature 25 deg.C, light period L10: D14. And observing the oviposition, hatching, larva and eclosion conditions of the Cereus flavus every day, and counting the parasitic rate. The above experimental treatments were all repeated 3 times.

TABLE 2 parasitism rates of different alternative hosts breeding Oak wasps

As can be seen from Table 2, when the wasp capped larvae are used as the alternative hosts to breed the Hedgersia sanguinea, the number of the wasps of a single host is higher than that of the natural host (the New Zea mairei), and the parasitic effect on the New Zerei mairei is equivalent to that of the natural state.

In light of the foregoing description of the preferred embodiment of the present invention, it is to be understood that various changes and modifications may be made by one skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.

Claims

1. An artificial breeding method of Cereus, which is characterized by comprising the following steps:

(1) cleaning the finger-shaped tube, sterilizing and drying in the air;

(3) inoculating fertilized female wasps of the Cerasifera into the finger-shaped tube, wherein the number ratio of the Cerasifera to the alternative hosts is 1:2-1:3, plugging the open end of the finger-shaped tube with a degreasing cotton after inoculation is finished, and culturing the finger-shaped tube in a climatic chamber; the small hornet is a Hedgersia wasp with calluses and a little hornet; the larvae are capped larvae; the culture conditions in the artificial climate box are as follows: the relative humidity is 30-70%, the temperature is 15-32 ℃, and the light period is L8-16: D16-8.

2. The artificial breeding method of the golden vespids, according to claim 1, characterized in that the pupae are milk white pupae.

3. The artificial breeding method of the golden vespids according to claim 1, wherein the culture conditions in the artificial climate box are as follows: the relative humidity is 40-60%, the temperature is 24-26 ℃, and the photoperiod is L10: D14.

4. The application of the Cereus went in preventing and controlling Nepalustris hutchuensis, is characterized in that the Cereus went cultured by the artificial breeding method of any one of claims 1 to 3 is subpackaged by finger tubes and delivered to a forest for release.