CN109832178A - A kind of culture medium and preparation method of Rosa roxburghii Tratt cuttage - Google Patents

Abstract

The invention discloses the culture mediums and preparation method of a kind of Rosa roxburghii Tratt cuttage, by weight, it is made of following raw materials, stalk: 10~20 parts, poplar bark: 10~20 parts, pulverized limestone: 2~6 parts, vermiculite power: 10~18 parts, glucose: 5~10 parts, vitamin B1: 0.5~1.5 part, calcium superphosphate: 5~9 parts, corn flour: 12~18 parts, heteroauxin: 0.3~0.8 part, methyl α-naphthyl acetate: 0.3~0.8 part, cortex albiziae: 6~12 parts, ammonium nitrate: 10~18 parts, potassium sulfate: 3~7 parts, 0.2-0.6 parts of lactic acid bacteria.Invention increases the high features of Rosa roxburghii Tratt cuttage survival rate.

Description

A kind of culture medium and preparation method of Rosa roxburghii Tratt cuttage

Technical field

The present invention relates to a kind of culture medium, the especially a kind of culture medium and preparation method of Rosa roxburghii Tratt cuttage.

Technical background

Rosa roxburghii Tratt, the title fruit of Cherokee rose, spinosity, therefore it is called " Rosa roxburghii Tratt ".The medical value of Rosa roxburghii Tratt, Li Shizhen (1518-1593 A.D.) is in " herbal guiding principle Mesh " in claim its " can direct oneself from boredom, remove food retention ".In terms of modern medicine viewpoint, all vitamin Cs, E lacks caused disease or symptom, thorn Pears have preventive and therapeutic action.Rosa roxburghii Tratt is the distinctive precious wild resource of Guizhou Plateau, is high nutrition, high medicinal comprehensive Health fruit.Rosa roxburghii Tratt is rich in a large amount of vitamin C and SOD (superoxide dismutase) and is rich in lot of anthocyanin, occupies the world It upper one cuts fruit, the hat of vegetables, it is 500 times higher than apple.Vitamin C can be fought as strong water soluble antioxidant Activity in vivo oxygen promotes skin immunity, reduces the skin inferior health problems such as obscure, yellow gas.Victoria C is as most common beauty simultaneously One of Bai Chengfen can effectively desalinate melanin, improve the colour of skin, promote skin transparency;And collagen is promoted to generate, enhance flesh Skin skin corium.SOD is a kind of active material derived from life entity, it can remove free radical extra in body, eliminates organism The harmful substance generated in metabolic processes goes back one health of human body, balance and great-hearted environment.Furthermore SOD pairs Treatment rheumatoid arthritis, dermatomyositis etc. have positive effect.To blood glucose is reduced, reduce blood consistency has well anthocyanidin Effect.Daily intaking a certain amount of anthocyanidin, to play endocrine metabolism normal, repairs islet function, activation islet cells again It is raw, make its normal secretions insulin, achievees the purpose that blood glucose steadily declines naturally.The note of thorn pear wine is brewed using Rosa roxburghii Tratt in history It carries, first appeared in verse of light 13 years (the 1833 Christian eras) Wu Songliang in " also appointing the west of Guizhou Province " of cleaning the street: " new wine Rosa roxburghii Tratt is invited one liquor-saturated, is satisfied With ashamed 3 years of fragrant rice ".Far away from bright, clear two court, Zhenxiong thorn pear wine is with regard to famous.The Ministry of official personnel Affairs in feudal China assistant minister of salty same time, Zhenxiong nationality are old Its thorn pear wine in the brewing of Zhenxiong state is often transported to city of Beijing private residence by dimension week, receives the princes, duckes and ministers in clear royal family.These are used to The wine visitor for judging famous brand of wine vintage wine, is also passionately devoted to Zhenxiong thorn pear wine, is profuse in praise, and " rubbing " wine of often finding an excuse to visit is drunk, for the moment It becomes a much-told tale.The brewing of thorn pear wine is aided with special Koji hair with Rosa roxburghii Tratt, glutinous rice, rock sugar, fresh leaf fat etc. for primary raw material Ferment after being formed by Particular craft brewing in history by awns portion, then fills wine with special soil pottery container and enters cellar, constant temperature storage.It puts Time general 3 years, 1 year or more was taken at the soonest.The cellar that expires out takes wine, and wine is in brownish red, gives out tempting strong faint scent.For a long time Drink in right amount, have reducing fever and causing diuresis, food digesting stomach fortifying, the scattered stasis of blood dispel heat effect;Since it also contains active material super oxygen one by one Dismutase can play the role of anti-cancer and anti-aging.It is raw material that Ribes burejense powder, which is with dried roxburgh rose fruit, with the grinding of ultralow temperature technique At.Nutrient content is abundant in fruit of Grossularia burejensis Berger, is classified as the necessary calcium of human physiological's health containing 18 kinds of amino acid and world organization, The microelements such as iron, magnesium, copper, iodine, brill.The special fresh Rosa roxburghii Tratt of the every 100g of Vitamin C content includes vitamin C and is up to 2500mg, is 550 times of apple, grape, it is 6-10 times higher than grandson monkey peach, and also rich in nutritional minerals matter such as polysaccharide, it is fruit worthy of the name King.Early in " Guizhou Province book ", " book on Chinese herbal medicine just Fang Erting collection ", " Traditional Chinese Medicine in Sichuan will ", " the civil recipe collection in Guizhou " with regard to Rosa roxburghii Tratt on the books Medicine, food effect.It has removing toxic substances, and stomach invigorating is helped digestion, invigorated blood circulation, changes silt, is hypoglycemic and unfold appearance, the function of moisturizing.

Currently, the cuttage of Rosa roxburghii Tratt haves the shortcomings that survival rate is low.

Summary of the invention:

The object of the present invention is to provide the culture mediums and preparation method of a kind of Rosa roxburghii Tratt cuttage.Invention increases Rosa roxburghii Tratt skewers The characteristics of inserting high survival rate.

In order to solve the above technical problems, the present invention is implemented with the following technical solutions:

A kind of culture medium of Rosa roxburghii Tratt cuttage is made of following raw materials by weight,

Stalk: 10~20 parts, poplar bark: 10~20 parts,

Pulverized limestone: 2~6 parts, vermiculite power: 10~18 parts,

Glucose: 5~10 parts, vitamin B1: 0.5~1.5 part,

Calcium superphosphate: 5~9 parts, corn flour: 12~18 parts,

Heteroauxin: 0.3~0.8 part, methyl α-naphthyl acetate: 0.3~0.8 part,

Cortex albiziae: 6~12 parts, ammonium nitrate: 10~18 parts,

Potassium sulfate: 3~7 parts, 0.2-0.6 parts of lactic acid bacteria.

The culture medium of Rosa roxburghii Tratt cuttage above-mentioned is made of following raw materials by weight,

Stalk: 12~18 parts, poplar bark: 12~18 parts,

Pulverized limestone: 3~5 parts, vermiculite power: 12~16 parts,

Glucose: 6~9 parts, vitamin B1: 0.6~1.2 part,

Calcium superphosphate: 6~8 parts, corn flour: 14~16 parts,

Heteroauxin: 0.7~0.7 part, methyl α-naphthyl acetate: 0.4~0.7 part,

Cortex albiziae: 7~11 parts, ammonium nitrate: 12~16 parts,

Potassium sulfate: 4~6 parts, 0.3-0.5 parts of lactic acid bacteria.

The culture medium of Rosa roxburghii Tratt cuttage above-mentioned is made of following raw materials by weight,

Stalk: 16 parts, poplar bark: 15 parts,

Pulverized limestone: 4 parts, vermiculite power: 14 parts,

Glucose: 7 parts, vitamin B1: 1.8 parts,

Calcium superphosphate: 7 parts, corn flour: 15 parts,

Heteroauxin: 0.5 part, methyl α-naphthyl acetate: 0.5 part,

Cortex albiziae: 8 parts, ammonium nitrate: 14 parts,

Potassium sulfate: 5 parts, 0.4 part of lactic acid bacteria.

The preparation method of the culture medium of Rosa roxburghii Tratt cuttage above-mentioned, is prepared according to the following steps:

(1) stalk is cut into the stalk segment of a length of 0.5-1.5cm long, it is 0.5- that poplar bark and cortex albiziae, which are ground into partial size, The particle of 1cm, by stalk segment and particle mix A product are spare；

(2) by the 1/3 of corn flour, A product, lactic acid bacteria and glucose gross weight mixing, be placed on that temperature is 25-30 °, humidity is Ferment 7-10h in the environment of 80%-90%, obtains B product；

(3) pulverized limestone, vermiculite power, calcium superphosphate, ammonium nitrate and potassium sulfate and B product are mixed, obtains C product；

(4) glucose of surplus, vitamin B1, heteroauxin, methyl α-naphthyl acetate are added suitable quantity of water and mixed, C product are added later and mix It is even, be packed into polyethylene plastic bag after mixing thoroughly, using 100 DEG C of steam normal-pressure sterilization to get.

Compared with prior art, the present invention first ferments stalk, poplar bark and cortex albiziae, obtains the group of short texture Structure is knitted, and decomposes and obtains crude fibre abundant, improves the gas permeability of culture medium；The present invention is directly put using glucose simultaneously Enter culture medium, be conducive to the direct absorption of Rosa roxburghii Tratt cuttage item, reduces the difficulty that cuttage item draws sugar, improve survival rate； Also contain heteroauxin and methyl α-naphthyl acetate in the present invention, be conducive to the generation of cuttage root, being conducive to, which enhances the absorption of cuttage item, supports The ability divided, further improves the survival rate of cuttage.

Specific embodiment:

Embodiment 1:

A kind of culture medium of Rosa roxburghii Tratt cuttage is made of following raw materials by weight,

Stalk: 16kg, poplar bark: 15kg,

Pulverized limestone: 4kg, vermiculite power: 14kg,

Glucose: 7kg, vitamin B1: 1.8kg,

Calcium superphosphate: 7kg, corn flour: 15kg,

Heteroauxin: 0.5kg, methyl α-naphthyl acetate: 0.5kg,

Cortex albiziae: 8kg, ammonium nitrate: 14kg,

Potassium sulfate: 5kg, lactic acid bacteria 0.4kg.

The culture medium of Rosa roxburghii Tratt cuttage above-mentioned is prepared according to the following steps:

(1) stalk is cut into the stalk segment of a length of 0.5-1.5cm long, it is 0.5- that poplar bark and cortex albiziae, which are ground into partial size, The particle of 1cm, by stalk segment and particle mix A product are spare；

(2) by the 1/3 of corn flour, A product, lactic acid bacteria and glucose gross weight mixing, be placed on that temperature is 25-30 °, humidity is Ferment 7-10h in the environment of 80%-90%, obtains B product；

(3) pulverized limestone, vermiculite power, calcium superphosphate, ammonium nitrate and potassium sulfate and B product are mixed, obtains C product；

(4) glucose of surplus, vitamin B1, heteroauxin, methyl α-naphthyl acetate are added suitable quantity of water and mixed, C product are added later and mix It is even, be packed into polyethylene plastic bag after mixing thoroughly, using 100 DEG C of steam normal-pressure sterilization to get.

Embodiment 2:

A kind of culture medium of Rosa roxburghii Tratt cuttage is made of following raw materials by weight,

Stalk: 10kg, poplar bark: 10kg,

Pulverized limestone: 2kg, vermiculite power: 10kg,

Glucose: 5kg, vitamin B1: 0.5kg,

Calcium superphosphate: 5kg, corn flour: 12kg,

Heteroauxin: 0.3kg, methyl α-naphthyl acetate: 0.3kg,

Cortex albiziae: 6kg, ammonium nitrate: 10kg,

Potassium sulfate: 3kg, lactic acid bacteria 0.2kg.

The culture medium of Rosa roxburghii Tratt cuttage above-mentioned is prepared according to the following steps:

(1) stalk is cut into the stalk segment of a length of 0.5-1.5cm long, it is 0.5- that poplar bark and cortex albiziae, which are ground into partial size, The particle of 1cm, by stalk segment and particle mix A product are spare；

(2) by the 1/3 of corn flour, A product, lactic acid bacteria and glucose gross weight mixing, be placed on that temperature is 25-30 °, humidity is Ferment 7-10h in the environment of 80%-90%, obtains B product；

(3) pulverized limestone, vermiculite power, calcium superphosphate, ammonium nitrate and potassium sulfate and B product are mixed, obtains C product；

(4) glucose of surplus, vitamin B1, heteroauxin, methyl α-naphthyl acetate are added suitable quantity of water and mixed, C product are added later and mix It is even, be packed into polyethylene plastic bag after mixing thoroughly, using 100 DEG C of steam normal-pressure sterilization to get.

Embodiment 3:

A kind of culture medium of Rosa roxburghii Tratt cuttage is made of following raw materials by weight,

Stalk: 20kg, poplar bark: 20kg,

Pulverized limestone: 6kg, vermiculite power: 18kg,

Glucose: 10kg, vitamin B1: 1.5kg,

Calcium superphosphate: 9kg, corn flour: 18kg,

Heteroauxin: 0.8kg, methyl α-naphthyl acetate: 0.8kg,

Cortex albiziae: 12kg, ammonium nitrate: 18kg,

Potassium sulfate: 7kg, lactic acid bacteria 0.6kg.

The culture medium of Rosa roxburghii Tratt cuttage above-mentioned is prepared according to the following steps:

(1) stalk is cut into the stalk segment of a length of 0.5-1.5cm long, it is 0.5- that poplar bark and cortex albiziae, which are ground into partial size, The particle of 1cm, by stalk segment and particle mix A product are spare；

(2) by the 1/3 of corn flour, A product, lactic acid bacteria and glucose gross weight mixing, be placed on that temperature is 25-30 °, humidity is Ferment 7-10h in the environment of 80%-90%, obtains B product；

(3) pulverized limestone, vermiculite power, calcium superphosphate, ammonium nitrate and potassium sulfate and B product are mixed, obtains C product；

(4) glucose of surplus, vitamin B1, heteroauxin, methyl α-naphthyl acetate are added suitable quantity of water and mixed, C product are added later and mix It is even, be packed into polyethylene plastic bag after mixing thoroughly, using 100 DEG C of steam normal-pressure sterilization to get.

Claims (4)

1. a kind of culture medium of Rosa roxburghii Tratt cuttage, which is characterized in that by weight, it is made of following raw materials,

Stalk: 10~20 parts, poplar bark: 10~20 parts,

Pulverized limestone: 2~6 parts, vermiculite power: 10~18 parts,

Glucose: 5~10 parts, vitamin B1: 0.5~1.5 part,

Calcium superphosphate: 5~9 parts, corn flour: 12~18 parts,

Heteroauxin: 0.3~0.8 part, methyl α-naphthyl acetate: 0.3~0.8 part,

Cortex albiziae: 6~12 parts, ammonium nitrate: 10~18 parts,

Potassium sulfate: 3~7 parts, 0.2-0.6 parts of lactic acid bacteria.

2. the culture medium of Rosa roxburghii Tratt cuttage as described in claim 1, which is characterized in that by weight, by following raw material structures At,

Stalk: 12~18 parts, poplar bark: 12~18 parts,

Pulverized limestone: 3~5 parts, vermiculite power: 12~16 parts,

Glucose: 6~9 parts, vitamin B1: 0.6~1.2 part,

Calcium superphosphate: 6~8 parts, corn flour: 14~16 parts,

Heteroauxin: 0.7~0.7 part, methyl α-naphthyl acetate: 0.4~0.7 part,

Cortex albiziae: 7~11 parts, ammonium nitrate: 12~16 parts,

Potassium sulfate: 4~6 parts, 0.3-0.5 parts of lactic acid bacteria.

3. the culture medium of Rosa roxburghii Tratt cuttage as claimed in claim 2, which is characterized in that by weight, by following raw material structures At,

Stalk: 16 parts, poplar bark: 15 parts,

Pulverized limestone: 4 parts, vermiculite power: 14 parts,

Glucose: 7 parts, vitamin B1: 1.8 parts,

Calcium superphosphate: 7 parts, corn flour: 15 parts,

Heteroauxin: 0.5 part, methyl α-naphthyl acetate: 0.5 part,

Cortex albiziae: 8 parts, ammonium nitrate: 14 parts,

Potassium sulfate: 5 parts, 0.4 part of lactic acid bacteria.

4. the preparation method of the culture medium of Rosa roxburghii Tratt cuttage as claimed in claim 3, it is characterised in that: make in the steps below It is standby:

(1) stalk is cut into the stalk segment of a length of 0.5-1.5cm long, it is 0.5- that poplar bark and cortex albiziae, which are ground into partial size, The particle of 1cm, by stalk segment and particle mix A product are spare；

(2) by the 1/3 of corn flour, A product, lactic acid bacteria and glucose gross weight mixing, be placed on that temperature is 25-30 °, humidity is Ferment 7-10h in the environment of 80%-90%, obtains B product；

(3) pulverized limestone, vermiculite power, calcium superphosphate, ammonium nitrate and potassium sulfate and B product are mixed, obtains C product；

(4) glucose of surplus, vitamin B1, heteroauxin, methyl α-naphthyl acetate are added suitable quantity of water and mixed, C product are added later and mix It is even, be packed into polyethylene plastic bag after mixing thoroughly, using 100 DEG C of steam normal-pressure sterilization to get.