CN109745477A - A kind of Chinese medicine composition and preparation method thereof for preventing and treating porcine reproductive and respiratory syndrome - Google Patents

Abstract

The Chinese medicine composition of the present invention for being used to prevent and treat porcine reproductive and respiratory syndrome, Chinese medicine including following parts by weight meter: 15-35 parts of the coptis, 5-20 parts of the dried immature fruit of citron orange, 15-30 parts of Poria cocos, 5-15 parts of semen coicis, 25-40 parts of semen ziziphi spinosae, 25-40 parts of Charred Triplet, 15-25 parts of Cortex Phellodendri, 5-25 parts of Cortex Magnoliae Officinalis, 35-50 parts of cape jasmine, 20-40 parts of rough gentian, 15-35 parts of Radix Isatidis, 5-20 parts of rheum officinale, 25-40 parts of elscholtiza, 35-50 parts of the tuber of pinellia, 25-40 parts of the shell of seaear, 25-40 parts of chrysanthemum, 15-35 parts of umbellate pore furgus, 10-30 parts of Radix Polygalae, 10-30 parts of radix scutellariae, 20-40 parts of oriental wormwood, 15-35 parts of Radix Codonopsis, 35-50 parts of radix rehmanniae recen, 30-50 parts of radix scrophulariae, Rhizoma Atractylodis Macrocephalae 5-20 Part, 25-40 parts of Radix Angelicae Sinensis, 5-25 parts of Radix Glycyrrhizae, 15-35 parts of gypsum, 15-35 parts of rhizoma anemarrhenae, 10-30 parts of Radix Salviae Miltiorrhizae, 15-30 parts of dried orange peel, Radix Ophiopogonis 5-25 part.The medicine is trembled with fear for porcine reproductive and respiratory syndrome, and poison closes lung, asthenia of renal yang disease type has fabulous therapeutic effect, and pure natural, antibiotic-free remains risk, and symptomatic treatment, preparation method is simple, is suitable for large-scale promotion and uses.

Description

A kind of Chinese medicine composition for preventing and treating porcine reproductive and respiratory syndrome and its Preparation method

Technical field:

The invention belongs to veterinary drug technical fields, and in particular to a kind of for preventing and treating porcine reproductive and respiratory syndrome Chinese medicine composition and preparation method thereof.

Background technique:

Porcine reproductive and respiratory syndrome (Porcine reproductive and respiratory syndrome, PRRS) be pig caused by virus a kind of breeding difficulty and respiratory system infectious disease.Since the ear of part sick pig is blue, Also known as " pig blue-ear disease ".This disease is propagated rapidly, mainly through respiratory tract infection, therefore when health pig is contacted with sick pig, as circle is raised It supports, frequently allocation and transportation, high concentration are easier to lead to the generation of this disease and prevalence.This disease can also vertical transmission.3 after boar infection~ Virus can be separated in 27d and 43d sperm collected, 7~14d can find virus from blood.To contain virulent essence Liquid inductance contaminates sow, sow can be caused to fall ill, PRRSV antibody can be detected after 21d.

But due to PRRS not only cause with other many pig breeding dysfunction disease (porcine parvovirus, porcine pseudorabies, Smoothing filter operator, Japanese B encephalitis, swine fever etc.) clinical symptom it is closely similar, and be easily mixed infection, while not Clinical symptom has very big difference again after infecting PRRS with pig farm, therefore makes a definite diagnosis and depend on laboratory diagnosis.It has been established at present a variety of The RT-PCR methods for increasing PRRSV gene more, and be widely used to face and examine detection.American type and Europe can be also distinguished by the method Type strain.It should be noted that in diagnosis and this disease and pig parvoviral, porcine pseudorabies, encephalitis B and Delayed onset swine fever etc. mutually reflected Not.

But the disease there is no specific medicament therapy at present, mainly takes comprehensive measures for prevention and control and antibiotic symptomatic treatment, deposits In the risk of antibiotic residue, and pig-pig infection breeding also has different disease type performances from breath syndrome virus, wherein It coughs, drinks loss of appetite, ear and the livid purple color of four limbs, sow, which often has greatly, returns feelings, and illness temperature of pig body increases, Chinese medical discrimination Cult poison violate lung inherently cough, runny nose, asthma, belong to tremble with fear poison close lung, asthenia of renal yang disease type.

Summary of the invention:

There are problems that antibiotic residue antibiotic treatment porcine reproductive and respiratory syndrome, the present invention provides a kind of use Lung, Chinese medicine composition of asthenia of renal yang disease type and preparation method thereof are closed in preventing and treating the cold poison of porcine reproductive and respiratory syndrome.

The present invention solves scheme used by its technical problem: one kind is integrated for preventing and treating pig breeding with breathing The Chinese medicine composition of sign, the Chinese medicine including following parts by weight meter: 15-35 parts of the coptis, 5-20 parts of the dried immature fruit of citron orange, 15-30 parts of Poria cocos, the heart of a lotus seed 5-15 parts of the seed of jog's tears, 25-40 parts of semen ziziphi spinosae, 25-40 parts of Charred Triplet, 15-25 parts of Cortex Phellodendri, 5-25 parts of Cortex Magnoliae Officinalis, 35-50 parts of cape jasmine, dragon 20-40 parts of gallbladder, 15-35 parts of Radix Isatidis, 5-20 parts of rheum officinale, 25-40 parts of elscholtiza, 35-50 parts of the tuber of pinellia, 25-40 parts of the shell of seaear, chrysanthemum 25-40 parts of flower, 15-35 parts of umbellate pore furgus, 10-30 parts of Radix Polygalae, 10-30 parts of radix scutellariae, 20-40 parts of oriental wormwood, 15-35 parts of Radix Codonopsis, radix rehmanniae recen 35-50 parts, 30-50 parts of radix scrophulariae, 5-20 parts of Rhizoma Atractylodis Macrocephalae, 25-40 parts of Radix Angelicae Sinensis, 5-25 parts of Radix Glycyrrhizae, 15-35 parts of gypsum, rhizoma anemarrhenae 15-35 Part, 10-30 parts of Radix Salviae Miltiorrhizae, 15-30 parts of dried orange peel, Radix Ophiopogonis 5-25 part.

Above-mentioned is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, including following parts by weight The Chinese medicine of meter: 15-25 parts of the coptis, 5-15 parts of the dried immature fruit of citron orange, 20-30 parts of Poria cocos, 5-10 parts of semen coicis, 30-40 parts of semen ziziphi spinosae, coke three 30-40 parts celestial, 15-20 parts of Cortex Phellodendri, 5-15 parts of Cortex Magnoliae Officinalis, 35-45 parts of cape jasmine, 20-30 parts of rough gentian, 15-25 parts of Radix Isatidis, rheum officinale 5-15 parts, 25-35 parts of elscholtiza, 35-45 parts of the tuber of pinellia, 25-35 parts of the shell of seaear, 25-35 parts of chrysanthemum, 15-25 parts of umbellate pore furgus, Radix Polygalae 10- 20 parts, 10-20 parts of radix scutellariae, 20-30 parts of oriental wormwood, 15-25 parts of Radix Codonopsis, 35-45 parts of radix rehmanniae recen, 30-40 parts of radix scrophulariae, Rhizoma Atractylodis Macrocephalae 5-15 Part, 30-40 parts of Radix Angelicae Sinensis, 5-15 parts of Radix Glycyrrhizae, 25-35 parts of gypsum, 15-25 parts of rhizoma anemarrhenae, 10-20 parts of Radix Salviae Miltiorrhizae, 20-30 parts of dried orange peel, wheat Winter 5-15 part.

Above-mentioned is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, including following parts by weight The Chinese medicine of meter: 20 parts of the coptis, 10 parts of the dried immature fruit of citron orange, 25 parts of Poria cocos, 10 parts of semen coicis, 30 parts of semen ziziphi spinosae, 30 parts of Charred Triplet, Cortex Phellodendri 25 Part, 10 parts of Cortex Magnoliae Officinalis, 35 parts of cape jasmine, 20 parts of rough gentian, 20 parts of Radix Isatidis, 15 parts of rheum officinale, 25 parts of elscholtiza, 40 parts of the tuber of pinellia, the shell of seaear 30 Part, 25 parts of chrysanthemum, 15 parts of umbellate pore furgus, 10 parts of Radix Polygalae, 15 parts of radix scutellariae, 20 parts of oriental wormwood, 15 parts of Radix Codonopsis, 40 parts of radix rehmanniae recen, radix scrophulariae 40 Part, 10 parts of Rhizoma Atractylodis Macrocephalae, 40 parts of Radix Angelicae Sinensis, 10 parts of Radix Glycyrrhizae, 25 parts of gypsum, 20 parts of rhizoma anemarrhenae, 15 parts of Radix Salviae Miltiorrhizae, 20 parts of dried orange peel, 10 parts of Radix Ophiopogonis.

The present invention also provides a kind of for preventing and treating the preparation side of the Chinese medicine composition of porcine reproductive and respiratory syndrome Method, comprising the following steps: weigh each Chinese medicine according to weight part ratio, put it into medicine decocting tank, the pure of 10 times of amounts is added into tank Water purification or 70% ethyl alcohol impregnate 1h, decoct 30min, and filtering obtains and decocts filtrate for the first time；The pure of 10 times of amounts is added into filter residue Water purification or 70% ethyl alcohol decoct 30min, filter to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, it is dry It is sprayed into powder；Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

The coptis in side, it is bitter, it trembles with fear, enters the heart, liver, stomach, large intestine channel, heat-clearing and damp-drying drug, purging fire for removing toxin, this product wants for heat-clearing and damp-drying drug Medicine, it is all to belong to damp and hot all cards, it can apply, especially most suitable with the card of stomach stagnation of damp-heat, clearing heat-fire effect is stronger, high with internal heat is controlled It contains, aphthae, three Jiao Jire control the hot tongue boil of the heart, are good at clearing heat and detoxicating；

The dried immature fruit of citron orange, it is bitter, it is slightly cold, enter spleen, stomach meridian, relieving stagnant Qi disperse accumulation, defaecation benefit diaphragm, with the taste stagnation of the circulation of vital energy is controlled, phlegm wet water is drunk caused Tripe distention and fullness in the abdomen can enhance stomach, intestines rhythmicity is wriggled with constipation with heat retention, tripe distention and fullness in the abdomen pain person is controlled, and be conducive to excrement and gas Discharge；

Poria cocos, it is sweet, it is light, put down, enter spleen, stomach, the heart, lung, kidney channel, eliminating dampness and diuresis, strengthening spleen and middle warmer, antitoxic heart-soothing and sedative, this product it is sweet and It is light, it is sweet can and in, infiltration can be seeped and be let out, water retention due to hypofunction of the spleen, and water, which is drunk, not to be changed, retention of water-damp, can invigorating the spleen but also dampness removing, energy complementary energy rushes down；

Semen coicis, it is sweet, it is light, it is slightly cold, enter spleen, lung, kidney channel, removing damp-heat, invigorating the spleen to arrest diarrhea, eliminating impediment, clearing lung-heat gold in this product Heat, wet, the edema swollen with harnessing the river of lower benefit stomach and intestine, sandstone heat gonorrhea, property slightly sweet flavor strengthen the spleen and stomach, and remove damp and clearing heat, easing joint movement；

Semen ziziphi spinosae, sweet, acid puts down, enters the heart, Liver Channel, mental-tranquilization, benefiting yin arrest sweating, this product nourishing heart yin, beneficial liver blood and calm the nerves, Being mainly used for heart-liver blood deficiency cannot nourish, so that restlessness, occurs in flaring up of deficient fire, arrest sweating benefiting yin are common to control abnormal sweating；

Charred Triplet, i.e. melanoidin malt, charred FRUCTUS CRATAEGI, stir-baked MASSA FERMENTATA MEDICI-NALIS, this three tastes medicine have the stagnant function of good disperse accumulationization, and melanoidin malt has The effect of good digestible starch group food；Charred FRUCTUS CRATAEGI is good at treating meat or greasy excessive caused food stagnation；Stir-baked MASSA FERMENTATA MEDICI-NALIS is then sharp In digestion rice and flour food, three medicines are shared, and can significantly enhance digestive function；

Cortex Phellodendri, it is bitter, tremble with fear, enter kidney, bladder, large intestine channel, clearing away damp-heat, purging intense heat poison, reducing the asthenic fever, this product have heat-clearing and damp-drying drug it Function, reducing the asthenic fever, with controlling fever due to yin deficiency；

Cortex Magnoliae Officinalis, it is bitter, it is acrid in flavour and warm in nature, enter spleen, stomach, large intestine channel, promoting qi circulation to remove dampness, relieving asthma, this product can be except stomach and intestine stagnant gas, eliminating dampness Spleen is transported, with controlling damp retention in middle-jiao, tripe distention and fullness in the abdomen, abdominal pain or the hiccup caused by the stagnation of the circulation of vital energy is unfavorable；

Cape jasmine, bitter, cold, return heart, lung, tri-jiao channel, purging fire for removing toxin, reducing fever and causing diuresis, cool blood, hemostasis, this product have purging fire for removing toxin Effect, it is kind clear away heart-fire, liver, tri-jiao channel heat, it is burning hot through it to be longer than clearing liver especially, clear three burnt fire and diuresis, and benefit liver and gallbladder damp-heat, cool blood Hemostasis, suitable for hematuria caused by blood-heat bleeding, hematochezia；

Rough gentian, it is bitter, it trembles with fear, enters liver, gallbladder, bladder meridian, heat-clearing and damp-drying drug, purge the green, this product energy heat-clearing and damp-drying drug is chiefly used in damp and hot Huang Subcutaneous ulcer urinates short red, eczema, rushes down excess-fire of the liver, and clear dampness-heat of liver channel is used for wind-heat of liver channel, red eye, swell pain for the key medicine for controlling irascibility；

Radix Isatidis, bitter, cold, return heart, stomach meridian are clearing heat and detoxicating, cool blood relieving sore-throat, are apt to removing summer-heat real heat fire toxin, and heat-clearing and cool blood are used Control maculopapulae due to toxic heat；

Rheum officinale, it is bitter, it trembles with fear, enters spleen, stomach, large intestine, liver, pericardium channel, stasis removing, purging fire and cooling blood, promoting blood circulation, this product is good at Cleanse sthenia gastropyrexia, constipation is stagnant, for the key medicine that bitter cold is captured, with control constipation with heat retention, abdominal pain rises and retires, real heat stop up it is stagnant, under can rushing down, It can expel the heat-evil again, promoting blood circulation is used for stagnation of blood stasis, and Qinghua is damp and hot, clearing heat and detoxicating；

Elscholtiza, pungent, tepor enter lung, stomach meridian, driving away summer heat inducing diaphoresis, dampness removing row water, and this product energy driving away summer heat inducing diaphoresis is chiefly used in exogenous disease wind Evil summer-heat and damp, lossless and incoordination between the spleen and the stomach card, tonneau water-wet for oedema, urinate unfavorable, sweating, antipyretic, diuresis；

The tuber of pinellia, acrid in flavour and warm in nature, returns spleen, stomach, lung channel is eliminating dampness and eliminating phlegm, stopping nausea and vomiting by lowering the adverse flow of QI, and help digestion dissipating bind, the pungent scattered temperate-dryness of this product, drops inverse The function of preventing or arresting vomiting is significant, can be used for regurgitation and emesis, abdominal distension, is especially suitable for removing dampness and eliminating phlegm to vomiting caused by drink and damp evil retardance is stopped, For the key medicine for controlling damp phlegm cough and asthma, it is suitable for cough with dyspnea, sputum is stopped up stagnant, help digestion dissipating bind, with controlling tripe distention and fullness in the abdomen；

The shell of seaear, it is salty, put down, enter Liver Channel, it is calming the liver, clear liver and improve vision, this product be good at it is calming the liver, be suitable for liver kidney yin Void, red eye, swell pain caused by liver-yang hyperactivity are the key medicine of calming the liver and improving eyesight, suitable for red eye, swell pain caused by the hot real example of liver, photophobia It sheds tears；

Chrysanthemum, sweet, bitter, slightly cold, return lung, Liver Channel, relieve heat heat-clearing are clear liver and improved vision, and body reaches clearly table, and gas floats clearly, cool in nature clear Heat, heat-clearing power are preferable；

Umbellate pore furgus, it is sweet, it is light, it puts down, enters kidney, bladder meridian, promoting diuresis for stranguria, dehumidify detumescence, there is preferable diuresis；

Radix Polygalae, pungent, bitter, tepor enters the heart, and lung channel, for having no peace of mind, restlessness, eliminating the phlegm is had one's ideas straightened out, dissipation carbuncle swells；

Radix scutellariae, it is bitter, it trembles with fear, enters lung, gallbladder, large intestine channel, heat-clearing and damp-drying drug, purging fire for removing toxin, miscarriage prevention, this product is longer than heat-clearing and damp-drying drug, main It is used for damp-heat dysentery, damp-warm syndrome, jaundice, heat gonorrhea let out clearly part of the body cavity above the diaphragm housing the heart and lungs excess fire, be good at especially with clearing lung-heat heat, also can be clearing heat and detoxicating, moreover it is possible to clear Hot miscarriage prevention；

Oriental wormwood, it is bitter, it is slightly cold, enter spleen, stomach, liver, gallbladder channel, clearing away damp-heat, sharp jaundice, this product hardship lets out decline, the special eliminating dampness and heat of function, Control diarrhea due to damp-heat；

Radix Codonopsis, sweet and neutral enter spleen, and lung channel, tonifying middle-Jiao and Qi, invigorating the spleen is promoted the production of body fluid, for the prolonged illness deficiency of vital energy, fatigue and weakness, deficiency syndrome of the lung asthma Promote, splenasthenic diarrhea, for rectal prolapse caused by collapse from qi deficiency, uterine prolapse；

Radix rehmanniae recen, it is sweet, it is bitter, it trembles with fear, enters the heart, liver, kidney channel, clearing heat and cooling blood, nourishing Yin and promoting production of body fluid, this product is with clearing heat and cooling blood and yin-nourishing Effect, cooling blood and hemostasis, what is caused for blood-heat bleeding goes out blood trouble, promotes the production of body fluid to quench thirst；

Radix scrophulariae, it is sweet, it is bitter, it is salty, tremble with fear, enter lung, stomach, kidney channel, clearing heat and nourishing yin moisturizes removing toxic substances, this product can clearing heat-fire and Nourish yin-fluid, giving consideration to both the incidental and fundamental, no matter heat toxin excess fire, yin asthenia generating intrinsic heat can be used, and removing toxic substances be moisturized, with controlling pharynx caused by flaring up of deficient fire Larynx swelling and pain, the uninteresting knot of saliva；

Rhizoma Atractylodis Macrocephalae, sweet, bitter, temperature enters spleen, stomach meridian is invigorated the spleen and benefited qi, eliminate dampness and have diuretic effect, strengthening exterior and reducing sweat, and this product is the weight invigorated the spleen and benefited qi Drug is wanted, deficiency of spleen-QI and stomach-QI is mainly used for, deficiency of food turgor caused by dysfunction of the spleen in transportation and transformation, fatigue and weakness is used for deficiency-cold in spleen and stomach, and tripe abdomen is cold Bitterly, diarrhea, invigorating the spleen to dry, and can Li Shui, can be used for retention of water-damp in the body or the excessive oedema of water-wet, invigorating qi for strengthening superficies, for exterior deficiency from Sweat, miscarriage prevention control threatened abortion；

Radix Angelicae Sinensis, sweet, pungent, bitter, temperature enters liver, spleen, the heart channel of Hang-Shaoyin.Benefiting blood and regulating blood circulation, promoting blood circulation and stopping pain, relax bowel and defecation, this product can mend Blood, and can invigorate blood circulation, it is used for weak syndrome of blood deficiency, promoting blood circulation and stopping pain；

Radix Glycyrrhizae, sweet and neutral enter 12 warps, and tonifying middle-Jiao and Qi is clearing heat and detoxicating, moistening lung to arrest cough, mitigates pharmacological property, has sweet slow moistening lung to stop The function of cough is wheezed with cough is controlled, lung cold cough and asthma or lung heat cough and asthma, can mitigate the high strong property of drug, have the function of coordinating the drug actions of a prescription；

Gypsum, pungent, sweet, Great Cold enter lung, and stomach meridian, clearing heat-fire, this product Great Cold has powerful clearing heat-fire effect, kind Clean air divides real heat, is used for the big heat of lung stomach, the real heats such as hyperpyrexia Kang Sheng card, and eliminating heat from the lung is used for cough with lung heat, and asthma is thirsty The excessive heat syndromes such as greedy drink, let out stomach energy, for the card such as stomach fire Kang Sheng；

Rhizoma anemarrhenae, it is bitter, it trembles with fear, enters lung, stomach, kidney channel, heat-clearing, enriching yin, moistening lung promotes the production of body fluid, and this product bitter cold not only lets out lung heat, but also clearing stomach Fire has the illness of real heat suitable for lung, stomach, and lung heat phlegm is thick, nourishing Yin and moistening lung, is used for deficiency of Yin hectic fever, deficiency syndrome of the lung cough caused by dryness, the greedy drink of pyreticosis；

Radix Salviae Miltiorrhizae, it is bitter, it is slightly cold, enter the heart, pericardium, Liver Channel, promoting blood circulation, cool blood to disappear carbuncle, nourishing blood and tranquilization, promoting blood circulation can use It is the disease suffered from a variety of extravasated blood, enters ying blood, restlessness for warm disease heat；

Dried orange peel, pungent, bitter, temperature enter spleen, lung channel, and regulating qi-flowing for strengthening spleen adjusts burnt mechanism of qi, gas row in smooth then to stop bitterly, gets along well for middle gas Caused by tripe swollen distention and fullness in the abdomen, loss of appetite, vomiting, diarrhea is eliminating dampness and eliminating phlegm, and phlegm wet blocks, the circulation of vital energy in the wrong direction is breathed with cough with controlling, and digestion is not It is good；

Radix Ophiopogonis, sweet, slight bitter is cool, enters lung, stomach, the heart channel of Hang-Shaoyin, heart clearing, lung moistening, nourishing the stomach to improve the production of body fluid, this product clearing heat and nourishing yin, moistening lung to arrest cough, For yin asthenia generating intrinsic heat, dry cough lacks phlegm, and nourishing the stomach to improve the production of body fluid is suitable for yin asthenia generating intrinsic heat, consumption of body fluid caused by febrile disease, thirsty greedy drink, dry constipation of intestines；

This product controls insufficiency of lung-QI,deficiency of lung-QI, the lungs cold of insufficiency type, heat-clearing and damp-drying drug, purging fire for removing toxin, relieving stagnant Qi disperse accumulation, defaecation benefit diaphragm, strengthening spleen and middle warmer, Antitoxic heart-soothing and sedative, removing damp-heat, mental-tranquilization, benefiting yin arrest sweating, disperse accumulationization is stagnant, promoting qi circulation to remove dampness, relieving asthma, and cool blood relieving sore-throat attacks product Promoting, promoting blood circulation, driving away summer heat inducing diaphoresis, stopping nausea and vomiting by lowering the adverse flow of QI, help digestion dissipating bind, calming the liver, and relieve heat heat-clearing is clear liver and improved vision, promoting diuresis for stranguria, The effect of dehumidify detumescence, and invigorating the spleen is promoted the production of body fluid, and nourishing Yin and promoting production of body fluid moisturizes removing toxic substances, invigorates the spleen and benefits qi, strengthening exterior and reducing sweat.

Specific embodiment:

Below with reference to embodiment, the invention will be further described.

Embodiment 1: it is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome described in the present embodiment, Chinese medicine including following parts by weight meter: coptis 20g, dried immature fruit of citron orange 10g, Poria cocos 25g, semen coicis 10g, semen ziziphi spinosae 30g, Charred Triplet 30g, Cortex Phellodendri 25g, Cortex Magnoliae Officinalis 10g, cape jasmine 35g, rough gentian 20g, Radix Isatidis 20g, rheum officinale 15g, elscholtiza 25g, tuber of pinellia 40g, shell of seaear 30g, Chrysanthemum 25g, umbellate pore furgus 15g, Radix Polygalae 10g, radix scutellariae 15g, oriental wormwood 20g, Radix Codonopsis 15g, radix rehmanniae recen 40g, radix scrophulariae 40g, Rhizoma Atractylodis Macrocephalae 10g, when Return 40g, Radix Glycyrrhizae 10g, gypsum 25g, rhizoma anemarrhenae 20g, Radix Salviae Miltiorrhizae 15g, dried orange peel 20g, Radix Ophiopogonis 10g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the pure water of 10 times of amounts is added into tank 1h is impregnated, 30min is decocted, filtering obtains and decocts filtrate for the first time；The volume fraction that 10 times of amounts are added into filter residue is 70% second Alcohol decocts 30m in, filters to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, is dried in vacuo into powder； Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 2: it is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome described in the present embodiment, Chinese medicine including following parts by weight meter: coptis 20g, dried immature fruit of citron orange 10g, Poria cocos 25g, semen coicis 8g, semen ziziphi spinosae 35g, Charred Triplet 35g, Cortex Phellodendri 22g, Cortex Magnoliae Officinalis 10g, cape jasmine 40g, rough gentian 25g, Radix Isatidis 20g, rheum officinale 10g, elscholtiza 30g, tuber of pinellia 40g, shell of seaear 30g, Chrysanthemum 30g, umbellate pore furgus 20g, Radix Polygalae 15g, radix scutellariae 15g, oriental wormwood 25g, Radix Codonopsis 20g, radix rehmanniae recen 40g, radix scrophulariae 35g, Rhizoma Atractylodis Macrocephalae 10g, when Return 35g, Radix Glycyrrhizae 10g, gypsum 30g, rhizoma anemarrhenae 20g, Radix Salviae Miltiorrhizae 15g, dried orange peel 25g, Radix Ophiopogonis 10g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the pure water of 10 times of amounts is added into tank 1h is impregnated, 30min is decocted, filtering obtains and decocts filtrate for the first time；The pure water that 10 times of amounts are added into filter residue decocts 30min, Filter to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, is dried in vacuo into powder；The drying of gained filter residue It crushes, is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 3: it is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome described in the present embodiment, Chinese medicine including following parts by weight meter: coptis 15g, dried immature fruit of citron orange 20g, Poria cocos 20g, semen coicis 5g, semen ziziphi spinosae 25g, Charred Triplet 40g, Cortex Phellodendri 15g, Cortex Magnoliae Officinalis 5g, cape jasmine 45g, rough gentian 30g, Radix Isatidis 15g, rheum officinale 5g, elscholtiza 25g, tuber of pinellia 50g, shell of seaear 35g, chrysanthemum Spend 25g, umbellate pore furgus 15g, Radix Polygalae 30g, radix scutellariae 10g, oriental wormwood 40g, Radix Codonopsis 35g, radix rehmanniae recen 35g, radix scrophulariae 30g, Rhizoma Atractylodis Macrocephalae 5g, Radix Angelicae Sinensis 40g, Radix Glycyrrhizae 15g, gypsum 35g, rhizoma anemarrhenae 25g, Radix Salviae Miltiorrhizae 10g, dried orange peel 30g, Radix Ophiopogonis 5g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, each Chinese medicine crushed one by one, 400 meshes is crossed respectively, by all powder End is uniformly mixed up to Chinese medicine composition powder of the present invention.

Embodiment 4: it is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome described in the present embodiment, Chinese medicine including following parts by weight meter: coptis 25g, dried immature fruit of citron orange 5g, Poria cocos 15g, semen coicis 10g, semen ziziphi spinosae 40g, Charred Triplet 25g, Cortex Phellodendri 20g, Cortex Magnoliae Officinalis 25g, cape jasmine 35g, rough gentian 20g, Radix Isatidis 25g, rheum officinale 20g, elscholtiza 35g, tuber of pinellia 35g, shell of seaear 40g, Chrysanthemum 35g, umbellate pore furgus 25g, Radix Polygalae 10g, radix scutellariae 20g, oriental wormwood 30g, Radix Codonopsis 15g, radix rehmanniae recen 50g, radix scrophulariae 40g, Rhizoma Atractylodis Macrocephalae 20g, when Return 30g, Radix Glycyrrhizae 5g, gypsum 25g, rhizoma anemarrhenae 15g, Radix Salviae Miltiorrhizae 20g, dried orange peel 15g, Radix Ophiopogonis 25g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the volume point of 10 times of amounts is added into tank Number is that 70% ethyl alcohol impregnates 1h, decocts 30min, and filtering obtains and decocts filtrate for the first time；The volume of 10 times of amounts is added into filter residue Score is that 70% ethyl alcohol decocts 30min, filters to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure at medicinal extract, very Sky is dried to powder；Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 5: it is a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome described in the present embodiment, Chinese medicine including following parts by weight meter: coptis 35g, dried immature fruit of citron orange 15g, Poria cocos 30g, semen coicis 15g, semen ziziphi spinosae 30g, Charred Triplet 30g, Cortex Phellodendri 25g, Cortex Magnoliae Officinalis 15g, cape jasmine 50g, rough gentian 40g, Radix Isatidis 35g, rheum officinale 15g, elscholtiza 40g, tuber of pinellia 45g, shell of seaear 25g, Chrysanthemum 40g, umbellate pore furgus 35g, Radix Polygalae 20g, radix scutellariae 30g, oriental wormwood 20g, Radix Codonopsis 25g, radix rehmanniae recen 45g, radix scrophulariae 50g, Rhizoma Atractylodis Macrocephalae 15g, when Return 25g, Radix Glycyrrhizae 25g, gypsum 15g, rhizoma anemarrhenae 35g, Radix Salviae Miltiorrhizae 30g, dried orange peel 20g, Radix Ophiopogonis 15g.

Preparation method: accurately weighing above-mentioned each Chinese medicine, put it into medicine decocting tank, and the volume point of 10 times of amounts is added into tank Number is that 70% ethyl alcohol impregnates 1h, decocts 30min, and filtering obtains and decocts filtrate for the first time；The pure of 10 times of amounts is added into filter residue Water decocts 30min, filters to obtain second of decoction filtrate；Merge filtrate twice, is concentrated under reduced pressure into medicinal extract, is dried in vacuo into powder； Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing pack.

Embodiment 6: effect of drugs measurement

(1) the traditional chinese medicine composition of the invention is to Marc-145 cytotoxicity assay

96 porocyte culture plates of Marc-145 cell monolayer are covered in preparation, and Chinese medicine composition of the invention is cultivated with DMEM After base is diluted according to multiple proportions method, make drug it is final concentration of (500g/mL, 250g/mL, 125g/mL, 62.5g/mL, 31.25g/mL、15.63g/mL、 7.81g/mL、3.95g/mL、1.98g/mL、0.99g/mL、0.49g/mL、0.25g/mL、 0.12g/mL、0.06g/mL、0.03g/mL、15.43mg/mL、7.71mg/mL、3.86 mg/mL、1.93mg/mL、0.97mg/ ML, 0.48mg/mL, 0.24mg/mL), it is added separately in cell hole, each drug concentration does 4 repetitions, in 5%CO₂、37 48h is cultivated under the conditions of DEG C, 96 orifice plates are taken out after 48h, and 20 μ L MTT solution (5 mg/mL) are added in every hole, continue to take after cultivating 8h Culture plate out is inhaled and abandons culture solution in hole, and 150 μ L dimethyl sulfoxides are added to every hole, sets low speed on shaking table and shakes 5min, OD_570nmUnder the conditions of measure the light absorption value in each hole, then calculate the maximum safe concentration of drug；

(2) measurement of the traditional chinese medicine composition of the invention to PRRSV inhibitory effect

The traditional chinese medicine composition of the invention is diluted according to coubling dilution, make drug it is final concentration of (500g/mL, 250g/mL、125g/mL、62.5g/mL、31.25g/mL、15.63 g/mL、7.81g/mL、3.95g/mL、1.98g/mL、 0.99g/mL、0.49g/mL、 0.25g/mL、0.12g/mL、0.06g/mL、0.03g/mL、15.43mg/mL、7.71mg/mL、 3.86mg/mL, 1.93mg/mL, 0.97mg/mL, 0.48mg/mL, 0.24mg/mL), according to 4 repetitions of each concentration, press respectively 100 μ L medical fluids are accessed according to every hole, access has been covered in 96 porocyte culture plates of single layer Marc-145 cell, 5%CO₂, 37 DEG C 1h is cultivated, medical fluid is abandoned；Add TCID₅₀Virus liquid every hole 100 μ L, 5%CO₂, 37 DEG C of culture 1h separately set cell controls and virus Control.5%CO₂, 37 DEG C of culture 5d, daily observation cell situation.The traditional chinese medicine composition of the invention is calculated on Marc-145 cell Concentration is blocked to the absorption of virus；

(3) the traditional chinese medicine composition of the invention is on Marc-145 cell to the inhibiting effect of virus

It uses and has covered with 96 porocyte plates of single layer Marc-145 cell as culture plate, according to 100 holes μ L/ by TCID₅₀Disease Venom accesses on culture plate, 5%CO₂, 37 DEG C of culture 1h, inhale abandon virus liquid, cleaned 3 times with PBS liquid, add doubling dilution Medical fluid, make drug it is final concentration of (500g/mL, 250g/mL, 125g/mL, 62.5g/mL, 31.25g/mL, 15.63g/mL, 7.81g/mL、3.95g/mL、1.98g/mL、0.99g/mL、0.49g/mL、 0.25g/mL、0.12g/mL、0.06g/mL、 0.03g/mL、15.43mg/mL、7.71mg/mL、3.86mg/mL、1.93mg/mL、0.97mg/mL、0.48mg/mL、0.24mg/ ML), according to 100 holes μ L/, each concentration is repeated 4 times, if cell controls group and virus control group.By inoculated cell culture Plate is put into 5%CO₂, 37 DEG C of culture 5d, daily observation cell situation；The traditional chinese medicine composition of the invention is calculated on Marc-145 cell To the inhibition concentration of virus.

(4) direct deactivation of the traditional chinese medicine composition of the invention on Marc-145 cell to virus

By TCID₅₀The virus liquid of concentration and the medical fluid of doubling dilution mix in equal volume, make the final concentration of (500g/ of drug mL、250g/mL、125g/mL、62.5g/mL、31.25g/mL、 15.63g/mL、7.81g/mL、3.95g/mL、1.98g/mL、 0.99g/mL、0.49g/mL、 0.25g/mL、0.12g/mL、0.06g/mL、0.03g/mL、15.43mg/mL、7.71mg/mL、 3.86mg/mL, 1.93mg/mL, 0.97mg/mL, 0.48mg/mL, 0.24mg/mL), 5%CO₂, 37 DEG C of culture 1h press respectively According to every 100 μ L of hole, each hole is repeated 4 times, and is inoculated into 96 orifice plates for covering with cell monolayer, while virus control wells, drug are arranged Control wells and blanc cell control, 5%CO₂, 37 DEG C of culture 5d, daily observation cell situation；Calculate the traditional chinese medicine composition of the invention To the direct inactivation concentration of virus on Marc-145 cell.

Measurement result is shown in Table 1.

The effect measuring result of 1 the traditional chinese medicine composition of the invention of table

As shown in Table 1, the traditional chinese medicine composition of the invention is 250g/ml to the maximum safe concentration of Marc-145 cell, i.e., in Drug composition concentration can carry out cell experiment, not have toxicity to cell in 250g/ml or less；Chinese medicine group through the invention Measurement of the object to PRRSV inhibitory effect is closed, the traditional chinese medicine composition of the invention blocks PRRSV that lesion does not occur to Marc-145 cell Cmin is 250g/ml；Measurement of the Chinese medicine composition on Marc-145 cell to the inhibiting effect of virus through the invention, It is 0.24mg/ml to the Cmin that lesion does not occur for Marc-145 cell that the traditional chinese medicine composition of the invention, which completely inhibits PRRSV,；It is logical It crosses the traditional chinese medicine composition of the invention to measure the direct deactivation of virus on Marc-145 cell, the traditional chinese medicine composition of the invention is complete Full inactivation PRRSV is 0.48mg/ml to the Cmin that lesion does not occur for Marc-145 cell.

Embodiment 7: clinical application effect

On October 12nd, 2015,600 pigs of one pig farm Zhang of Henan, wherein sow 30,75 hair of growing and fattening pigs are sick. Sick pig is mainly shown as cough, drinks loss of appetite, the symptoms such as ear and the livid purple color of four limbs；Sow, which often has greatly, returns feelings, illness pig Body temperature is increased to 39~41 DEG C, according to clinical symptoms and molecular diagnosis, is diagnosed as pig blue-ear disease, belongs to cold poison and closes lung, asthenia of renal yang Type.5 tons are watered with the traditional chinese medicine composition of the invention 1kg, twice a day, 6d is used in conjunction, pig is allowed freely to drink；The 5th day state of an illness of medication obtains To control, symptom disappears within the 6th day, and swinery restores normal.

Claims (4)

1. a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, which is characterized in that including following heavy Measure the Chinese medicine of part meter: 15-35 parts of the coptis, 5-20 parts of the dried immature fruit of citron orange, 15-30 parts of Poria cocos, 5-15 parts of semen coicis, 25-40 parts of semen ziziphi spinosae, burnt Three 25-40 parts of celestial beings, 15-25 parts of Cortex Phellodendri, 5-25 parts of Cortex Magnoliae Officinalis, 35-50 parts of cape jasmine, 20-40 parts of rough gentian, 15-35 parts of Radix Isatidis, rheum officinale 5-20 parts, 25-40 parts of elscholtiza, 35-50 parts of the tuber of pinellia, 25-40 parts of the shell of seaear, 25-40 parts of chrysanthemum, 15-35 parts of umbellate pore furgus, Radix Polygalae 10- 30 parts, 10-30 parts of radix scutellariae, 20-40 parts of oriental wormwood, 15-35 parts of Radix Codonopsis, 35-50 parts of radix rehmanniae recen, 30-50 parts of radix scrophulariae, Rhizoma Atractylodis Macrocephalae 5-20 Part, 25-40 parts of Radix Angelicae Sinensis, 5-25 parts of Radix Glycyrrhizae, 15-35 parts of gypsum, 15-35 parts of rhizoma anemarrhenae, 10-30 parts of Radix Salviae Miltiorrhizae, 15-30 parts of dried orange peel, wheat Winter 5-25 part.

2. it is according to claim 1 a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, It is characterized in that, the Chinese medicine including following parts by weight meter: 15-25 parts of the coptis, 5-15 parts of the dried immature fruit of citron orange, 20-30 parts of Poria cocos, semen coicis 5-10 Part, 30-40 parts of semen ziziphi spinosae, 30-40 parts of Charred Triplet, 15-20 parts of Cortex Phellodendri, 5-15 parts of Cortex Magnoliae Officinalis, 35-45 parts of cape jasmine, rough gentian 20-30 Part, 15-25 parts of Radix Isatidis, 5-15 parts of rheum officinale, 25-35 parts of elscholtiza, 35-45 parts of the tuber of pinellia, 25-35 parts of the shell of seaear, chrysanthemum 25-35 Part, 15-25 parts of umbellate pore furgus, 10-20 parts of Radix Polygalae, 10-20 parts of radix scutellariae, 20-30 parts of oriental wormwood, 15-25 parts of Radix Codonopsis, 35-45 parts of radix rehmanniae recen, 30-40 parts of radix scrophulariae, 5-15 parts of Rhizoma Atractylodis Macrocephalae, 30-40 parts of Radix Angelicae Sinensis, 5-15 parts of Radix Glycyrrhizae, 25-35 parts of gypsum, 15-25 parts of rhizoma anemarrhenae, Radix Salviae Miltiorrhizae 10- 20 parts, 20-30 parts of dried orange peel, Radix Ophiopogonis 5-15 part.

3. it is according to claim 1 a kind of for preventing and treating the Chinese medicine composition of porcine reproductive and respiratory syndrome, It is characterized in that, the Chinese medicine including following parts by weight meter: 20 parts of the coptis, 10 parts of the dried immature fruit of citron orange, 25 parts of Poria cocos, 10 parts of semen coicis, semen ziziphi spinosae 30 parts, 30 parts of Charred Triplet, 25 parts of Cortex Phellodendri, 10 parts of Cortex Magnoliae Officinalis, 35 parts of cape jasmine, 20 parts of rough gentian, 20 parts of Radix Isatidis, 15 parts of rheum officinale, elscholtiza 25 parts, 40 parts of the tuber of pinellia, 30 parts of the shell of seaear, 25 parts of chrysanthemum, 15 parts of umbellate pore furgus, 10 parts of Radix Polygalae, 15 parts of radix scutellariae, 20 parts of oriental wormwood, Radix Codonopsis 15 Part, 40 parts of radix rehmanniae recen, 40 parts of radix scrophulariae, 10 parts of Rhizoma Atractylodis Macrocephalae, 40 parts of Radix Angelicae Sinensis, 10 parts of Radix Glycyrrhizae, 25 parts of gypsum, 20 parts of rhizoma anemarrhenae, 15 parts of Radix Salviae Miltiorrhizae, 20 parts of dried orange peel, 10 parts of Radix Ophiopogonis.

4. a kind of Chinese medicine composition for being used to prevent and treat porcine reproductive and respiratory syndrome as described in claims 1 or 2 or 3 Preparation method, which comprises the following steps: weigh each Chinese medicine according to weight part ratio, each Chinese medicine be put into medicine decocting tank In, the pure water or 70% ethyl alcohol that 10 times of amounts are added into tank impregnate 1h, decoct 30min, and filtering obtains and decocts filtrate for the first time；To The pure water or 70% ethyl alcohol that 10 times of amounts are added in filter residue decoct 30 min, filter to obtain second of decoction filtrate；Merging is filtered twice Liquid is concentrated under reduced pressure into medicinal extract, and drying is sprayed into powder；Gained filter residue dries pulverizing is uniformly mixed with powder obtained by filtrate, packing dress Bag.