CN109734536A - A kind of ecological agent and preparation method thereof promoting desertification plant growth - Google Patents
A kind of ecological agent and preparation method thereof promoting desertification plant growth Download PDFInfo
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Abstract
The invention belongs to ecological agent technical fields, are related to a kind of ecological agent and preparation method thereof for promoting desertification plant growth；Ecological agent includes 60-70 parts of starch, 10-15 parts of humic acid, 2-5 parts of calcium superphosphate, 8-10 parts of microbial bacterial agent, 10-20 parts of algal polysaccharides；Microbial bacterial agent is the brevibacterium Brevibacterium sp.TS22 tamed by low altitude area and room temperature, deposit number are as follows: CGMCC 16576；Microbial bacterial agent may also include phosphate solubilizing bacteria, and weight is the 0~20% of microbial bacterial agent；The preparation step of ecological agent is as follows: the starting components of ecological agent being put into blender according to parts by weight, are stirred to react, ecological agent is obtained；Ecological agent of the invention can individually or agroforestry organic fertilizer uses；Operation of the present invention is easy, and production cost is low, so that the emergence rate of desertification plant improves 50% or more.
Description
Technical field
The invention belongs to ecological agent technical fields, and in particular to it is a kind of promote desertification plant growth ecological agent and
Preparation method.
Background technique
Soil desertification refers to good soil or available soil becomes containing husky many soil or soil or even becomes
The process in desert.Soil desertification main process is wind erosion and wind deposit process.In desert surrounding area, due to vegetation deterioration or
Overgrazing or the reclamation of wasteland are farmland, and soil becomes drying because of dehydration, and grogs dispersion is lost by wind, fine particle content drop
It is low.And after wind-force or the location weakened, dust storm particle gradual builds up upper soll layer and makes soil desertification.Therefore, soil
Desertify includes the wind erosion process of grassland soil and the aeolian sediments process in location farther out.The large area of desertification of land is spread
Desertification is one of Global Environmental Problems of most serious.
There is 20% land on the earth at present by the threat of desertification；Not only area is big for China's desertification, distribution
Range is wide, and endangers seriously, has a far reaching influence.It is estimated that as desertification it is annual caused by direct economic loss up to 54,000,000,000
Member, and indirect economic loss is 2~3 times or even 10 times of direct economic loss or more.On the one hand, desertification destroys ecological ring
Border threatens human survival；Meanwhile desertification deteriorates biological quality, species abundance reduces, and constitutes serious prestige to bio-diversity
The side of body；In addition, desertification destroyed land resource, makes to cause the agriculture-stock production underproduction even exhausted using soil reduction, quality decline
It receives；In addition, desertification also usually does great damage to water conservancy projects, such as silt invades reservoir, pile things on feed ditch, raw to agricultural
Production affects.
Soil desertification seriously affects plant seed germination and development, growth.One side soil desertification can reduce vegetable seeds
Germination percentage；On the other hand, salt ionic concentration is higher in prone soil, moisture content is low, therefore generates water stress to plant,
Ionic stress is also generated simultaneously, destroys intracellular ionic equilibrium；Lead to the accumulation of active oxygen in plant, to injure cell
Even it is killed；In addition, growing plants in prone soil, often generates Nutrient Stress, the growth and development of such plant
It will be abnormal.Soil desertification is to inhibit the growth of tissue and organ, accelerated development to the overall performance of plant growth and development
Journey shortens nutrient growth and reproduction period.
So being attempted to develop and utilize sandy land from the directions such as agricultural, chemistry, biology；According to ameliorative measure
Difference, different effects can be obtained for the development and utilization of desertification.Improvement desertification soil can pass through green manuring, Shi You
The crops such as machine fertilizer or kind rice improve its salt.Although these methods have certain effect to the improvement for the soil that desertifies,
It is that effect is unstable, and in practical application, this has virtually been significantly greatly increased in the investment of a large amount of human and material resources and financial resources
The cost of item measure.
Summary of the invention
In view of the above-mentioned problems, present invention seek to address that one of described problem, a kind of desertification plant growth that promotes is provided
Ecological agent and preparation method thereof, specific technical solution are as follows:
Present invention firstly provides a kind of ecological agents for promoting desertification plant growth, including following starting components and again
Measure number, 60-70 parts of starch, 10-15 parts of humic acid, 2-5 parts of calcium superphosphate, 8-10 parts of microbial bacterial agent, algal polysaccharides 10-20
Part；
The microbial bacterial agent is the brevibacterium (Brevibacterium sp.TS22) derived from Qinghai-Tibet Platean through too low sea
It pulls out and tames and obtain with room temperature, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows:
CGMCC 16576；
The mode of the low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture
In base, in secondary culture repeatedly under room temperature；LB culture medium includes tryptone 10g/L, yeast extract 5g/L, sodium chloride
5g/L, pH 7.4.
The microbial bacterial agent further includes phosphate solubilizing bacteria, and the phosphate solubilizing bacteria is bacillus megaterium (Bacillus
Megaterium), pseudomonad (Pseudomonas sp.) or agrobacterium (Agrobacterium sp.)；
Need to tame culture before the phosphate solubilizing bacteria use；The domestication culture is that phosphorus decomposing bacterial strain is inoculated into Phos training
Base is supported, in 20-25 DEG C；Strain passage culture is carried out under low temperature environment；The Phos culture medium prescription are as follows: glucose 10g/L,
(NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L,
MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of sterilizing 20min of 5g/L, pH.
It is described by low altitude area and room temperature domestication obtain brevibacterium and domestication culture after phosphate solubilizing bacteria when in use, need through
Cross taming again for condition corresponding with area's main environmental conditions will be used, then be respectively as follows: will be by low altitude area for the condition tamed
It is inoculated into LB culture medium with the brevibacterium of room temperature domestication, the secondary culture repeatedly at 20-25 DEG C；It will be solved after domestication culture
Phosphorus bacterium is inoculated into LB culture medium, the secondary culture repeatedly at 10-20 DEG C；Wherein LB culture medium prescription is as follows: tryptone
10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
The weight of the phosphate solubilizing bacteria is the 0-20% of microbial bacterial agent.
The present invention also provides a kind of preparation methods of ecological agent for promoting desertification plant growth, the specific steps are as follows:
The starting components of ecological agent are put into blender according to parts by weight, the revolving speed of blender is set in room
Warm item is reacted, and the ecological agent of desertification plant growth is promoted.
The revolving speed of the blender is set as 100-300r/min；Reaction time is 10-30min
The ecological agent can also be used in mixed way with agricultural organic fertilizer.
Beneficial effects of the present invention:
(1) ecological agent prepared by the present invention with can effectively facilitating stony desertification plant growth；After adding ecological agent processing,
The emergence rate of plant can be improved 50% or more, and the biomass of plant improves 47% or more；In addition, ecology system prepared by the present invention
Agent can also be used in combination with agricultural organic fertilizer, can preferably improve the growth of plant.
(2) operation of the present invention is easy, and production cost is low, has highly important theory and practice meaning.
Specific embodiment
In the present invention, the number of bacillus megaterium is CICC20665, is purchased from the management of Chinese industrial Microbiological Culture Collection
Center；The number of pseudomonad is CGMCC10242, purchased from China Committee for Culture Collection of Microorganisms's common micro-organisms
The heart；The number of agrobacterium is CICC10214, is purchased from Chinese industrial Microbiological Culture Collection administrative center；
Brevibacterium Brevibacterium sp.TS22, it is commonly micro- to be preserved in China Committee for Culture Collection of Microorganisms
Bio-Centers, address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3；Deposit number are as follows: CGMCC 16576；Preservation date: 2018
On October 12, in.
Brevibacterium sp.TS22 sequence information:
>CCCCGCCATCGGCTTCGTTCGAATGCCATGCATAGCGACTCGCACGCCAAGCGTG CGTCCCTGAGC
CATGATCAAACTCTATGAGTAACACGTGGGCAACCTGCCTATAAGACT GGGATAACTTCGGGAAACCGGAGCTAA
TACCGGATACGTTCTTTTCTCGCATGAGAGAA GATGGAAAGACGGTTTACGCTGTCACTTATAGATGGGCCCGCG
GCGCATTAGCTAGTTG GTGAGGTAATGGCTCACCAAGGCGACGATGCGTAGCCGACCTGAGAGGGTGATCGGCC
ACACTGGGACTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTAGGGAATCTTCC GCAATGGACGAAAGTCT
GACGGAGCAACGCCGCGTGAACGAAGAAGGCCTTCGGGTC GTAAAGTTCTGTTGTTAGGGAAGAACAAGTACCAG
AGTAACTGCTGGTACCTTGACGGT ACCTAACCAGAAAGCCACGGCTAACTACGTGCCAGCAGCCGCGGTAATACG
TAGGTGGC AAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCAGGTGGTTCCTTAAGTCTGATG TGAAAGC
CCACGGCTCAACCGTGGAGGGTCATTGGAAACTGGGGAACTTGAGTGCAGA AGAGGAAAGTGGAATTCCAAAGTG
TAGCGGTGAAATGCGTAGAGATTTGGAGGAACAC CAGTGGCGAAGGCGACTTTCTGGTCTGTAACTGACACTGAG
GCGCGAAAGCGTGGGGA GCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGAGTGCTAAGTGTTAG
AGGGTTTCCGCCCTTTAGTGCTGCAGCTAACGCATTAAGCACTCCGCCTGGGGAGTACG GCCGCAAGGCTGAAAC
TCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGT GGTTTAATTCGAAGCAACGCGAAGAACCTTACC
AGGTCTTGACATCCTCTGACAACCCT AGAGATAGGGCTTTCCCCTTCGGGGGACAGAGTGACAGGTGGTGCATGG
TTGTCGTCAG CTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTTGATCTTAGTTG CCAGC
ATTCAGTTGGGCACTCTAAGGTGACTGCCGGTGACAAACCGGAGGAAGGTGGG GATGACGTCAAATCATCATGCC
CCTTATGACCTGGGCTACACACGTGCTACAATGGATGG TACAAAGGGCTGCAAACCTGCGAAGGTAAGCGAATCC
CATAAAGCCATTCTCAGTTCGG ATTGCAGGCTGCAACTCGCCTGCATGAAGCCGGAATCGCTAGTAATCGCGGAT
CAGCAT GCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCC
Embodiment 1:
A kind of ecological agent promoting desertification plant growth, including following starting components and parts by weight, starch 68
Part, 10 parts of humic acid, 2 parts of calcium superphosphate, 10 parts of microbial bacterial agent, 20 parts of algal polysaccharides, microbial bacterial agent further include domestication training
Bacillus megaterium after supporting, weight are the 10% of microbial bacterial agent；
Microbial bacterial agent be derived from Qinghai-Tibet Platean brevibacterium (Brevibacterium sp.TS22) by low altitude area and
Room temperature is tamed and is obtained, and is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC
16576；The mode of low altitude area and room temperature domestication are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium,
In secondary culture repeatedly under room temperature, the brevibacterium for adapting to low altitude area and normal temperature condition is obtained；
The domestication culture of bacillus megaterium is to be inoculated into Phos culture medium, and strain passage training is carried out under 20 environment
It supports；The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O
0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)25g/L, pH 7.2,115
DEG C sterilizing 20min.
Bacillus megaterium is using after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
When, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: will be through too low sea
The brevibacterium tamed with room temperature and the bacillus megaterium after domestication culture are pulled out, is inoculated into LB culture medium respectively, in 20-25
Secondary culture repeatedly at DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/
L adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method for the ecological agent promoting desertification plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 180r/min, and temperature is room temperature, reaction
Time is 22min, and the ecological agent for promoting desertification plant growth is made.Ecological agent and agricultural organic fertilizer be by weight when application
Than uniformly being mixed for 1:99.
Embodiment 2:
A kind of ecological agent promoting desertification plant growth, including following starting components and parts by weight, starch 60
Part, 10 parts of humic acid, 2 parts of calcium superphosphate, 8 parts of microbial bacterial agent, 20 parts of algal polysaccharides, microbial bacterial agent further includes soil bar
Bacterium, weight are the 20% of microbial bacterial agent.；The microbial bacterial agent is the brevibacterium derived from Qinghai-Tibet Platean
(Brevibacterium sp. TS22) is obtained by low altitude area and room temperature domestication, is preserved in Chinese microorganism strain preservation pipe
Reason committee common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: will be isolated from
The brevibacterium of Qinghai-Tibetan plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtains adapting to low altitude area
With the brevibacterium of normal temperature condition；
The domestication culture of agrobacterium is to be inoculated into Phos culture medium, and strain passage culture is carried out under 25 DEG C of environment；
The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/
L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of 5g/L, pH go out
Bacterium 20min；
It is described to tame the brevibacterium obtained by low altitude area and room temperature and tame the agrobacterium after cultivating when in use,
It need to be tamed again by corresponding with area's main environmental conditions will be used condition, then be respectively as follows: will be through too low for the condition tamed
The brevibacterium of height above sea level and room temperature domestication is inoculated into LB culture medium, the secondary culture repeatedly at 20-25 DEG C；It will be by domestication culture
Inoculating agrobacteria is into LB culture medium afterwards, the secondary culture repeatedly at 10-20 DEG C；Wherein LB culture medium prescription is as follows: pancreas egg
White peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilizations with NaOH
20min。
A kind of preparation method for the ecological agent promoting desertification plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 200r/min, and temperature is room temperature, reaction
Time is 20min, and the ecological agent for promoting desertification plant growth is made.Ecological agent and agricultural organic fertilizer be by weight when application
Than uniformly being mixed for 2:98.
Embodiment 3:
A kind of ecological agent promoting desertification plant growth, including following starting components and parts by weight, starch 65
Part, 15 parts of humic acid, 2 parts of calcium superphosphate, 8 parts of microbial bacterial agent, 10 parts of algal polysaccharides, microbial bacterial agent further includes huge gemma
Bacillus, weight are the 20% of microbial bacterial agent；The microbial bacterial agent is the brevibacterium derived from Qinghai-Tibet Platean
(Brevibacterium sp.TS22) is obtained by low altitude area and room temperature domestication, is preserved in Chinese microorganism strain preservation management
Committee's common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: blueness will be isolated from
The brevibacterium of hiding plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtain adapting to low altitude area and
The brevibacterium of normal temperature condition；
The domestication culture of bacillus megaterium is to be inoculated into Phos culture medium, and strain passage training is carried out under 20 environment
It supports；The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O
0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)25g/L, pH 7.2,115
DEG C sterilizing 20min.
Bacillus megaterium is using after the brevibacterium obtained by low altitude area and room temperature domestication and domestication culture
When, taming again for condition corresponding with area's main environmental conditions will be used, then the condition tamed need to be passed through are as follows: will be through too low sea
The brevibacterium tamed with room temperature and the bacillus megaterium after domestication culture are pulled out, is inoculated into LB culture medium respectively, in 20-25
Secondary culture repeatedly at DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/
L adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method for the ecological agent promoting desertification plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 100r/min, and temperature is room temperature, reaction
Time is 30min, and the ecological agent for promoting desertification plant growth is made.
Embodiment 4:
A kind of ecological agent promoting desertification plant growth, including following starting components and parts by weight, starch 63
Part, 10 parts of humic acid, 2 parts of calcium superphosphate, 5 parts of microbial bacterial agent, 20 parts of algal polysaccharides, microbial bacterial agent further includes false unit cell
Bacterium, weight are the 10% of microbial bacterial agent；The microbial bacterial agent is the brevibacterium (Brevibacterium derived from Qinghai-Tibet Platean
Sp.TS22 it) is obtained by low altitude area and room temperature domestication, is preserved in China Committee for Culture Collection of Microorganisms's commonly micro- life
Object center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication are as follows: the short of Qinghai-Tibetan plateau soil will be isolated from
Bacillus is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, obtains the quarter butt for adapting to low altitude area and normal temperature condition
Bacterium；
The domestication culture of pseudomonad is to be inoculated into Phos culture medium, and strain passage culture is carried out under 20 environment；Institute
State Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O 0.5g/L,
KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)27.2,115 DEG C of 5g/L, pH sterilizings
20min；
It is described to tame the brevibacterium obtained by low altitude area and room temperature and tame the pseudomonad after cultivating when in use,
It need to be tamed again by corresponding with area's main environmental conditions will be used condition, then be respectively as follows: will be through too low for the condition tamed
The brevibacterium of height above sea level and room temperature domestication is inoculated into LB culture medium, the secondary culture repeatedly at 10-20 DEG C；It will be by domestication culture
Pseudomonad afterwards is inoculated into LB culture medium, the secondary culture repeatedly at 10-20 DEG C；Wherein LB culture medium prescription is as follows: pancreas
Peptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L adjust pH to 7.4,121 DEG C of high pressure steam sterilizations with NaOH
20min。
A kind of preparation method for the ecological agent promoting desertification plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 250r/min, and temperature is room temperature, reaction
Time is 15min, and the ecological agent for promoting desertification plant growth is made.
Embodiment 5:
A kind of ecological agent promoting desertification plant growth, including following starting components and parts by weight, starch 60
Part, 12 parts of humic acid, 4 parts of calcium superphosphate, 9 parts of microbial bacterial agent, 15 parts of algal polysaccharides, the microbial bacterial agent are derived from Qinghai-Tibet
The brevibacterium (Brevibacterium sp.TS22) on plateau obtains by low altitude area and room temperature domestication, is preserved in China Microbiological
Culture presevation administration committee common micro-organisms center, deposit number are as follows: CGMCC 16576；The mode of low altitude area and room temperature domestication
Are as follows: the brevibacterium for being isolated from Qinghai-Tibetan plateau soil is inoculated into LB culture medium, in secondary culture repeatedly under room temperature, is obtained
Adapt to the brevibacterium of low altitude area and normal temperature condition；
The brevibacterium obtained by low altitude area and room temperature domestication when in use, need to pass through and will use the main ring in area
Condition corresponding condition in border is tamed again, then the condition tamed are as follows: is inoculated into the brevibacterium tamed by low altitude area and room temperature
In LB culture medium, the secondary culture repeatedly at 10-20 DEG C；Wherein LB culture medium prescription is as follows: tryptone 10g/L, yeast mention
Object 5g/L, sodium chloride 10g/L are taken, adjusts pH to 7.4,121 DEG C of high pressure steam sterilization 20min with NaOH.
A kind of preparation method for the ecological agent promoting desertification plant growth, the specific steps are as follows:
Material is put into blender to mix, the revolving speed of blender is set as 280r/min, and temperature is room temperature, reaction
Time is 12min, and the ecological agent for promoting desertification plant growth is made.
Embodiment effect:
Field trial is to fertilizer functional verification:
Summer in testing field in the Gansu Lanzhou selection more consistent examination desertification of condition, is divided into 18 1 meter × 1 meters
Block, by above-mentioned 5 embodiments and each three design iterations of blank control, be prepared 100 grams of preparation of Example 1~5,
It is even to be mixed into soil, each 100, green vegetables seed are sowed, normal watering Irrigation counts thinning after emergence rate, then 10 days, respectively after 10 days
Stay 50 plants；Harvest randomly chooses each 20 plants of titles gross weight after 30 days again.Control group does not add ecological agent and the processing of agricultural organic fertilizer,
Remaining step is identical.Statistical result is described in table 1 below.
1 embodiment effect statistical form of table
As can be seen that the effect of embodiment 1-5 is substantially better than the effect of control group from above-mentioned table 1；Add ecological agent
After processing, the emergence rate of plant can be improved 50% or more, and the biomass of plant improves 47% or more, this explanation is of the invention
Formula is lower can to reach the growth for promoting desertification plant.In addition, from 1 result of table it has also been discovered that being properly added agricultural organic fertilizer
The growth-promoting effect of plant can preferably be improved；The emergence rate of plant can be improved 66% or so, and the biomass of plant improves
73% or so.
Illustrate: above embodiments are only to illustrate the present invention and not limit the technical scheme described by the invention；Therefore,
Although this specification is referring to above-mentioned each embodiment, the present invention has been described in detail, the common skill of this field
Art personnel should be appreciated that and still can modify to the present invention or equivalent replacement；And all do not depart from spirit of the invention and
The technical solution and its improvement of range, should all cover in scope of the presently claimed invention.
Sequence table
<110>Jiangsu University
<120>a kind of ecological agent and preparation method thereof for promoting desertification plant growth
<160> 1
<170> SIPOSequenceListing 1.0
<210> 2
<211> 1384
<212> DNA
<213>brevibacterium (Brevibacterium sp.)
<400> 2
ccccgccatc ggcttcgttc gaatgccatg catagcgact cgcacgccaa gcgtgcgtcc 60
ctgagccatg atcaaactct atgagtaaca cgtgggcaac ctgcctataa gactgggata 120
acttcgggaa accggagcta ataccggata cgttcttttc tcgcatgaga gaagatggaa 180
agacggttta cgctgtcact tatagatggg cccgcggcgc attagctagt tggtgaggta 240
atggctcacc aaggcgacga tgcgtagccg acctgagagg gtgatcggcc acactgggac 300
tgagacacgg cccagactcc tacgggaggc agcagtaggg aatcttccgc aatggacgaa 360
agtctgacgg agcaacgccg cgtgaacgaa gaaggccttc gggtcgtaaa gttctgttgt 420
tagggaagaa caagtaccag agtaactgct ggtaccttga cggtacctaa ccagaaagcc 480
acggctaact acgtgccagc agccgcggta atacgtaggt ggcaagcgtt gtccggaatt 540
attgggcgta aagcgcgcgc aggtggttcc ttaagtctga tgtgaaagcc cacggctcaa 600
ccgtggaggg tcattggaaa ctggggaact tgagtgcaga agaggaaagt ggaattccaa 660
agtgtagcgg tgaaatgcgt agagatttgg aggaacacca gtggcgaagg cgactttctg 720
gtctgtaact gacactgagg cgcgaaagcg tggggagcaa acaggattag ataccctggt 780
agtccacgcc gtaaacgatg agtgctaagt gttagagggt ttccgccctt tagtgctgca 840
gctaacgcat taagcactcc gcctggggag tacggccgca aggctgaaac tcaaaggaat 900
tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc 960
ttaccaggtc ttgacatcct ctgacaaccc tagagatagg gctttcccct tcgggggaca 1020
gagtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc 1080
gcaacgagcg caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact 1140
gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct 1200
gggctacaca cgtgctacaa tggatggtac aaagggctgc aaacctgcga aggtaagcga 1260
atcccataaa gccattctca gttcggattg caggctgcaa ctcgcctgca tgaagccgga 1320
atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac 1380
cgcc 1384
Claims (9)
1. a kind of ecological agent for promoting desertification plant growth, which is characterized in that including following starting components and parts by weight
Number, 60-70 parts of starch, 10-15 parts of humic acid, 2-5 parts of calcium superphosphate, 8-10 parts of microbial bacterial agent, 10-20 parts of algal polysaccharides；
The microbial bacterial agent is the brevibacterium Brevibacterium sp.TS22 derived from Qinghai-Tibet Platean, is tamed and dociled by low altitude area and room temperature
Change and obtain, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, deposit number are as follows: CGMCC 16576.
2. a kind of ecological agent for promoting desertification plant growth according to claim 1, which is characterized in that the low sea
Pull out the condition with room temperature domestication are as follows: brevibacterium Brevibacterium sp.TS22 is inoculated into LB culture medium, in room temperature item
Secondary culture repeatedly under part；The LB culture medium includes tryptone 10g/L, yeast extract 5g/L, and sodium chloride 5g/L, pH are
7.4。
3. a kind of ecological agent for promoting desertification plant growth according to claim 1, which is characterized in that micro- life
Object microbial inoculum further includes the phosphate solubilizing bacteria after domestication culture, and the weight of phosphate solubilizing bacteria is the 0- of microbial bacterial agent after the domestication culture
20%；The domestication culture is that phosphorus decomposing bacterial strain is inoculated into Phos culture medium, and bacterial strain biography is carried out under 10-20 DEG C of low temperature environment
It is commissioned to train feeding；The Phos culture medium prescription are as follows: glucose 10g/L, (NH4)2SO40.5g/L, NaCl 3g/L, MgSO4·7H2O
0.5g/L, KCl 0.5g/L, FeSO4·7H2O 0.03g/L, MnSO40.03g/L, Ca3(PO4)25g/L, pH 7.2,115
DEG C sterilizing 20min.
4. a kind of ecological agent for promoting desertification plant growth according to claim 3, which is characterized in that the phosphorus decomposing
Bacterium is bacillus megaterium, pseudomonad or agrobacterium.
5. a kind of ecological agent for promoting desertification plant growth according to claim 2 or 3, which is characterized in that described
Phosphate solubilizing bacteria when in use, need to pass through and will use after the brevibacterium and domestication culture of low altitude area and room temperature domestication acquisition
Main environmental conditions corresponding condition in area's is tamed again, then the condition tamed are as follows: the quarter butt that will be tamed by low altitude area and room temperature
Bacterium and the phosphate solubilizing bacteria after domestication culture, are inoculated into LB culture medium, the secondary culture repeatedly at 20-25 DEG C respectively；Wherein LB
Culture medium prescription is as follows: tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L, adjusts pH to 7.4,121 with NaOH
DEG C high pressure steam sterilization 20min.
6. a kind of preparation method of ecological agent for promoting desertification plant growth according to claim 1, feature exist
In, the specific steps are as follows: the starting components of ecological agent are put into blender according to parts by weight, blender is set
Revolving speed is reacted in room temperature item, is promoted the ecological agent of desertification plant growth.
7. a kind of preparation method of ecological agent for promoting desertification plant growth according to claim 6, feature exist
In the revolving speed of the blender is set as 100-300r/min；Reaction time is 10-30min.
8. by it is described in claim 1 it is a kind of promote desertification plant growth ecological agent be used to promote desertification plant growth
Purposes, which is characterized in that it is described promote desertification plant growth ecological agent be used alone or agroforestry organic fertilizer make
With.
9. purposes according to claim 8, which is characterized in that the ecological agent for promoting desertification plant growth is compound
Agricultural organic fertilizer is in use, promoting the ecological agent of desertification plant growth and the mass ratio of agricultural organic fertilizer is 1-2:98-99.
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