CN106416835B - Utilize the method for bacillus atrophy bacillus BA-7 prevention and treatment Cruciferae clubroot - Google Patents
Utilize the method for bacillus atrophy bacillus BA-7 prevention and treatment Cruciferae clubroot Download PDFInfo
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- CN106416835B CN106416835B CN201610585068.XA CN201610585068A CN106416835B CN 106416835 B CN106416835 B CN 106416835B CN 201610585068 A CN201610585068 A CN 201610585068A CN 106416835 B CN106416835 B CN 106416835B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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Abstract
The present invention provides a kind of methods using atrophy bacillus BA-7 prevention and treatment Cruciferae clubroot, this method includes following either method: method one: by atrophy bacillus BA-7 bacterium solution, root irrigation is carried out to crop in cruciferae with the amount of each every plant of 80-100ml, it at least handles three times, the processing time is the 1st day, the 7th day, the 15th day after planting；Method two: by atrophy bacillus BA-7 bacterium solution, spraying treatment is carried out to crop in cruciferae with the amount of every square meter 150-200ml, is at least handled three times, the processing time is the 1st day, the 7th day, the 14th day after planting；The crop in cruciferae includes rape, Chinese cabbage, broccoli；The classification naming of the atrophy bacillus is Bacillus atrophaeus BA-7, and China typical culture collection center is preserved on December 21st, 2015, and deposit number is CCTCC NO:M 2015765.
Description
Technical field
The invention belongs to biological pesticide technical fields, and in particular to prevent and treat Cruciferae root using atrophy bacillus BA-7
The method for the disease that swells.
Background technique
In recent years, clubroot is increasingly subject to the harm of crop in cruciferae the attention of people, and actively prevents and treats it
Method has carried out continuous research and has groped.Wherein, the research and development of biocontrol microorganisms are one of research hotspots therein.
However, in the prior art, have the bacterial strain of good control efficiency very little and excessively single crop in cruciferae,
It is concentrated mainly on bacillus subtilis (such as bacillus subtilis XF-1 and bacillus subtilis M3) and bacillus amyloliquefaciens is (such as
Bacillus amyloliquefaciens Bam22).
Therefore, seek other can efficiently prevent and treat the biocontrol microorganisms of crop in cruciferae clubroot and find suitable user
Method becomes this field technical problem urgently to be resolved.
Summary of the invention
In view of the shortcomings of the prior art, atrophy bacillus BA-7 prevention and treatment ten is utilized the purpose of the present invention is to provide a kind of
The method of Zi Hua section clubroot, the method comprising the steps of:
Method one: by atrophy bacillus BA-7 bacterium solution, with the amount of each every plant of 80-100ml to crop in cruciferae into
Row root irrigation is at least handled three times, and the processing time is the 1st day, the 7th day, the 15th day after planting；
Method two: atrophy bacillus BA-7 bacterium solution carries out crop in cruciferae with the amount of every square meter 150-200ml
Spraying treatment is at least handled three times, and the processing time is the 1st day, the 7th day, the 14th day after planting；
The crop in cruciferae includes rape, Chinese cabbage, broccoli；
The classification naming of the atrophy bacillus was Bacillus atrophaeus BA-7, on December 21st, 2015
It is preserved in China typical culture collection center, preservation address is wuchang, wuhan Luo Jia Shan China typical culture collection center
(Wuhan University), deposit number are CCTCC NO:M 2015765.
As shown in the embodiment of the invention, by after strain culturing of the present invention 24 hours, and the method for the present invention is utilized, 1000 times
Dilution have up to 88.5% (5 groups of parallel laboratory test average value) rapeseed clubroot control efficiency.
The mechanism for realizing said effect is that bacterial strain of the present invention itself, which has pathogen, inhibits and kill well work
With so that the incidence of rapeseed clubroot is greatly lowered.In addition, bacterial strain of the present invention is for the straw in farmland as shown in experimental example 1
There is good decomposed effect can have at normal temperature after applying strain culturing dilution of the present invention for stalk or other crop wastes
Decomposing straw or other crop wastes are imitated, and the pathogen therein that will hide oneself releases, and passes through pathogen and atrophy gemma
The direct contact of bacillus further realizes comprehensive effect of the bacterial strain of the present invention to pathogen in soil.Meanwhile the stalk after decomposition
Or other crop wastes can also provide more organic matters for the growth of next season crop, promote the healthy growth of crops.
In general, the decomposed fermentation of agricultural crop straw mostly uses anaerobism mode to carry out pile fermentation, it is not only time-consuming and laborious,
And it is easy to pollute surrounding enviroment.Bacterial strain of the present invention can be decomposed to stalk progress under aerobic conditions, without that will gather in
Stalk afterwards carries out centralized collection and carries out stack retting, it is only necessary to spread fertilizer over the fields in farmland zymocyte liquid of the present invention, bacterium solution is allowed to contact agriculture
Crop material can realize the decomposed and its later use to stalk.Meanwhile when applying external source stalk, even if in external source stalk
Band pathogen, according to the above description, it will be understood that the present invention is equally able to achieve good control efficiency.
Therefore, those skilled in the art kill work to the inhibition of pathogen well it can be appreciated that bacterial strain of the present invention not only has
With, while the quick composting to stalk can also be realized under aerobic conditions, so that next season rape is significantly from pathogen
The growth infected again and promote rape.
Under above-mentioned basis, the present invention has studied the method handled using bacterial strain of the present invention farmland, may be implemented
Crop in cruciferae farmland is handled to significantly improve the protection effect of crop.
It is worth noting that also very outstanding for the control efficiency of other Cruciferae clubroots.
The application concentration of biocontrol bacterial strain bacillus subtilis XF-1 known to the public is very high in the prior art, and once
Application concentration is too low, and control efficiency will be greatly lowered, and significantly limits its practical application.
Other common biocontrol bacterial strains are also mostly bacillus subtilis.Although the present inventor has found one plant before this
There is the bacillus amyloliquefaciens Bam22 of fine control efficiency to crop in cruciferae clubroot, widened the field cruciate flower
The strain range of section's biocontrol bacterial strain, however before this invention, the prior art does not occur that also height can be sought from atrophy bacillus
The report of the bacterial strain of effect prevention and treatment crop in cruciferae clubroot and corresponding instruction.
In addition, the method for the present invention can promote bacterial strain prevention and treatment effect of the present invention as shown in the embodiment of the present invention and comparative example
The performance of fruit.
Preferably, the bacterial concentration of the atrophy bacillus BA-7 bacterium solution is 6-8 × 106cfu/ml。
Specific embodiment:
The present invention is specifically described below by embodiment, it is necessary to which indicated herein is that following embodiment is
For the present invention to be further detailed, it should not be understood as limiting the scope of the invention, the technology in the field is ripe
Practice some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still falls within protection scope of the present invention.
Embodiment 1
(1) culture of bacterial strain
By atrophy bacillus BA-7 after solid medium culture, picking single colonie is placed in fluid nutrient medium, in
30-32 DEG C of concussion is trained 12 hours, revolving speed 220rpm, as seed liquor；The ingredient of the fluid nutrient medium is by weight percent
Meter include: yeast extract 0.3%, beef extract 0.2%, peptone 0.8%, NaCl 0.3-0.6%, surplus be water, pH=
7.0-7.2；
Step 1) gains are placed in fermentor and carry out fermentation 24 hours, tank temperature is 30 DEG C, and fermentor mixing speed is
250rpm；The ingredient of culture medium in fermentor includes: bean powder 4.5%, peptone 0.12%, glucose by mass percentage
0.6%, starch 2.5%, manganese sulfate 0.1%, ferrous sulfate 0.06%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1%, defoaming agent
0.01%, surplus is water, pH=7.0-7.4.
(2) biocontrol effect is tested
1000 times will be diluted by atrophy bacillus BA-7 bacterium solution obtained by the above method, with every plant 80 every time of amount to cross
Flower section crop carries out root irrigation, and three times, the processing time is the 1st day, the 7th day, the 15th day after planting for processing.Note: undiluted
The concentration of bacterium solution is about 6-8 × 109cfu/ml.Tested crop is rape, Chinese cabbage and broccoli.Each experimental group sets 5 parallel groups,
It is averaged.
Severity Scaling:
0 grade: without tumour, root system development is normal for root；
1 grade: 1-30% root a little tumour, have small nodositas lump, be mutually not connected to；
2 grades: 31-60% root raw tumour, have big nodositas or spherical lump, be connected with each other；
3 grades: 61-100% root raw tumour, expand and form spindle tumor, big tumor mass extends to hypocotyl,
Plant is short and small, stops growing.
Disease index=[∑ (diseased plant numbers at different levels × opposite value of series)/highest level × investigation total strain number] × 100.
Relative control effect=control the disease index of (control disease index-processing disease index) × 100/.
Experimental result is as shown in table 1.
Table 1
Note: the disease index of clear water control is 64%.
Embodiment 2
Except control method is to make atrophy bacillus BA-7 bacterium solution to Cruciferae with the amount of every square meter 150-200ml
Object carries out spraying treatment, at least handles three times, and the processing time is the 1st day, the 7th day, the 14th day after planting；Remaining and embodiment
1 is consistent.Gained control efficiency, compared with embodiment 1 is resulting, maximum deviation is no more than 3%.
Comparative example
Bushing method for root are as follows: 100ml is poured to every plant of crop when transplanting, was poured every 7 days once, was poured again every 14 days primary.Remaining
It is consistent with embodiment 1.
Experimental result is as shown in table 2.
Table 2
Note: the disease index of clear water control is 62%.
Experimental example 1
Farmland: Rapeseed Field；
One discharge of bacteria agent flusher is installed on straw harvesting machine, for microbial inoculum to be sprayed to agriculture while gathering in stalk
Field.
Harvesting crushes stalk in farmland, and to using in farmland atrophy bacillus BA-7 microbial inoculum, amount of application is 2,000,000,000,000
A bacterium/mu.The microbial inoculum is atrophy bacillus BA-7 fermentation liquid, and bacterial concentration is 6-9 × 109cfu/ml。
After processing 45 days, the detection of stalk weight-loss ratio (full name is straw biological amount weight-loss ratio) is carried out, weight-loss ratio is
61.3%.
Claims (2)
1. utilizing the method for atrophy bacillus BA-7 prevention and treatment Cruciferae clubroot, which is characterized in that the method includes such as
Lower either method:
Method one: atrophy bacillus BA-7 bacterium solution fills crop in cruciferae with the amount of each every plant of 80-100ml
Root processing, is at least handled three times, and the processing time is the 1st day, the 7th day, the 15th day after planting；
Method two: atrophy bacillus BA-7 bacterium solution is sprayed crop in cruciferae with the amount of every square meter 150-200ml
Processing is at least handled three times, and the processing time is the 1st day, the 7th day, the 14th day after planting；
The crop in cruciferae includes one of rape, Chinese cabbage, broccoli；
The classification naming of the atrophy bacillus was Bacillus atrophaeus BA-7, in preservation on December 21 in 2015
In China typical culture collection center, deposit number is CCTCC NO:M 2015765.
2. the method according to claim 1, wherein the bacterial concentration of the atrophy bacillus BA-7 bacterium is 6-
8×106cfu/ml。
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| CN107418910B (en) * | 2017-05-05 | 2020-08-25 | 昆明理工大学 | Biological agent for preventing and treating clubroot of cruciferous crops and application thereof |
| CN109673343A (en) * | 2019-03-07 | 2019-04-26 | 贵州省植物保护研究所 | A kind of integrated approach of prevention and control Cruciferae clubroot |
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| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
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| CN101416641A (en) * | 2008-09-12 | 2009-04-29 | 云南农业大学 | Biological preparation capable of preventing and treating cruciferae club root and use thereof |
| CN103281905A (en) * | 2010-12-21 | 2013-09-04 | 拜尔作物科学有限合伙公司 | Sandpaper mutants of bacillus and methods of their use to enhance plant growth, promote plant health and control diseases and pests |
| CN102732469A (en) * | 2012-07-12 | 2012-10-17 | 云南农业大学 | Bacillus methylotrophicus and application thereof |
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| CN105230664A (en) * | 2015-11-02 | 2016-01-13 | 四川省农业科学院植物保护研究所 | Application of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae |
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