CN109699537A - A kind of mating system of Stichopus variegatus (Sempen) artificial seed - Google Patents
A kind of mating system of Stichopus variegatus (Sempen) artificial seed Download PDFInfo
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- CN109699537A CN109699537A CN201810027951.6A CN201810027951A CN109699537A CN 109699537 A CN109699537 A CN 109699537A CN 201810027951 A CN201810027951 A CN 201810027951A CN 109699537 A CN109699537 A CN 109699537A
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Abstract
The present invention provides the mating system of Stichopus variegatus (Sempen) artificial seed a kind of, including parent participation cultivation, artificial stimulation adopt ovum, incubating oosperm, larval culture, collect seedling, young ginseng is cultivated, children ginseng is cultivated.Wherein, the present invention explores the breeding method that the ovum best opportunity is adopted in Stichopus variegatus (Sempen) artificial stimulation and ovum method and Stichopus variegatus (Sempen) auricularia, young ginseng, children's ginseng are adopted in stimulation, and optimize that Stichopus variegatus (Sempen) is young, the young ginseng process collection of falling pond tool, significantly improve young, young ginseng survival rate, parent participation induced spawning success rate 100% of the present invention, first ear larval culture planting percent 1.5-2.5%, effectively solves the high technical problem of Stichopus variegatus (Sempen) nursery difficulty by one square metre indoor raising of seedling pond nurture body long 2000,3-8cm seed or so.
Description
Technical field
The invention belongs to aquaculture fields, and in particular to a kind of mating system of Stichopus variegatus (Sempen) artificial seed.
Background technique
Stichopus variegatus (Sempen), scientific name: Stichopus variegatus, be Stichopodidae stichopus japonicus belong to marine animal, popular name " yellow meat ",
" side's ginseng ", its meat thickness are tender, are a kind of good beche-de-mers.The protein content 73.81% of Stichopus variegatus (Sempen), fat content
1.51%, total amino acid content 66.46%, unsaturated fatty acid content 39.25%, content of polyunsaturated fatty acid 5.56%.Tool
Have high protein, low fat feature, have very high nutritive value, healthcare function and economic value, product at home market for not
It should ask.In the world, Stichopus variegatus (Sempen) is also the high-grade aquatic products of a kind of important edible tropical sea cucumber type and foreign exchange earning.Geography point
Cloth: Taiwan, Weizhou Island, Guangxi, Guangdong Nao Zhoudao, Hainan Island and the Xisha Islands；West is from Madagascar, Zanzibar and Red sea
It rises, arrives Caroline eastwards, South Japan is arrived in north, and south is distributed to the Australia island Luo Dehao.
But current Stichopus variegatus (Sempen) seed breeding technical research, domestic other than the present inventor carries out news report, state
Second related Stichopus variegatus (Sempen) seed breeding was inside not yet met successfully to report.Be primarily present following two aspects problem: one, parent participation is pierced
Swash that adopt ovum success rate low, it is difficult to obtain a large amount of fertilized eggs；Two, 5 pairs of orbicule individual percentage of big auricularia stage appearance are low,
Abnormal development is that doliolaria percentage is low；Three, young children's ginseng is easy to appear ginseng body atrophy, exanthemv and autolysis, young children
It is low to join cultivating survival rate.
Summary of the invention
It is an object of the invention to overcome deficiency in the prior art, the breeding side of Stichopus variegatus (Sempen) artificial seed a kind of is provided
Ovum success rate height is adopted in method, stimulation, and young children's cultivating survival rate is high, cultivates high-quality ginseng Stichopus variegatus (Sempen) seed.
The present invention is implemented as follows:
A kind of mating system of Stichopus variegatus (Sempen) artificial seed, which comprises the following steps:
(1) parent participation is cultivated:
Fish natural Stichopus variegatus (Sempen), select ginseng body it is full, be parent participation without injured individual, put in a suitable place to breed in indoor cement pit or outdoor pond
It is cultivated in the pool, bottom of pond places things concealed；Using sargassum thunbergii powder, sargassum powder, stichopus japonicus parent participation mixed feed and ooze as bait, daily
Dusk bait throwing in 1 time, daily ration, feeding quantity is the 2-3% of parent participation weight；Indoor cement pit, which is cultivated, keeps continuous flowing water and inflation, periodically removes
Bottom of pond residual bait and excrement；Outdoor ponds are changed water 1 time every other day；
(2) ovum is adopted in artificial stimulation:
In Stichopus variegatus (Sempen) spawning season, select to carry out artificial stimulation in the front and back 3 days of the lunar calendar every month junior one to adopt ovum.
(3) incubating oosperm:
After smart ovum is discharged in parent participation, parent participation is pulled out with the dab net that nylon wire makes, is changed with 300 mesh sieve tulles case siphons
Water, changes water 1-2 times, each quantity of exchanged water 50-70%, and fertilized eggs are adopted in ovum pond in primary stimuli hatches；
(4) larval culture:
Then incubating oosperm development is put in the first ear young in nursery pond after the first ear young, to collect the just ear young and trains
It educates；Using Chaetoceros muelleri, Isochrysis galbana, spirulina powder and yeast as bait, daily bait throwing in 2 times；The young puts the 1st to the 3rd in a suitable place to breed
It, adds the 9-11% sand filter seawater of seedling water daily, changes water 20-30% after the 4th day daily；
(5) it collects seedling:
Polyethylene corrugated plate is launched when the big ear larval metamorphosis of 10-20% is doliolaria to collect seedling；After dispensing corrugated plating extremely
During doliolaria attachment finishes, using Chaetoceros muelleri, Isochrysis galbana, spirulina powder, yeast and bacillus as bait, often
Its bait throwing in 2 times；Stop changing water during collecting seedling, be finished to doliolaria attachment, starting flowing water improves water quality；
(6) young ginseng is cultivated:
Doliolaria settlement and metamorphosis be young ginseng after 10 days in, in addition to feeding spirulina, yeast and bacillus, start
Feed young ginseng mixed feed, sargassum thunbergii powder, sargassum powder and ooze, morning and at dusk each throwing 1 time, miniflow water and continuous charge item
It is cultivated under part；Young Shenfu the rear 11st to the 40th day, stop throwing spirulina and yeast, morning and each at dusk throw 1 time；Miniflow water and company
It is cultivated under continuous venting condition；At interval of 5-7 days removing bottom of pond residual baits and excrement 1 time, young ginseng is collected using 40 mesh sieve tulles casees, and
The young ginseng of collection is moved on in nursery pond in time；As young ginseng growth 1.0cm long up to body, children's ginseng breeding phase is initially entered；
(7) children's ginseng is cultivated:
Using children's ginseng mixed feed, sargassum thunbergii powder, sargassum powder, ooze and bacillus as bait, morning and dusk respectively throw 1
It is secondary；It is cultivated under the conditions of miniflow water and continuous charge；At interval of 3-5 days removing bottom of pond residual baits and excrement 1 time, using 20 mesh sieve tulles
Case collects children's ginseng；Culture about 40 days.
Preferably, step (1) are as follows: the selection natural Stichopus variegatus (Sempen) of weight 400-850g is put in a suitable place to breed in indoor cement pit or outdoor pond
It is cultivated in the pool, indoor pond breeding density 5-8 head/m2, outdoor ponds breeding density 1-2 head/m2, bottom of pond placement things concealed；Daily
Dusk bait throwing in 1 time, daily ration of feeding is the 2-3% of parent participation weight, wherein in parts by weight, the nursing bait of parent participation includes
22-27 parts of sargassum thunbergii powder, 22-27 parts of sargassum powder, 38-42 parts of stichopus japonicus parent participation mixed feed and 8-12 parts of ooze；Indoor cement
Pond, which is cultivated, keeps continuous flowing water and inflation, and day streamflow is to cultivate 2-4 times of water body, periodically removes bottom of pond residual bait and excrement；It is outdoor
Pond is changed water 1 time every other day, and day quantity of exchanged water is the 10-20% of pond waters；Seawater salinity 26-33, dissolved oxygen content 5mg/L or more.
It is further preferred that in step (1), in parts by weight, parent participation feed bait include 25 parts of sargassum thunbergii powder,
25 parts of sargassum powder, 40 parts of stichopus japonicus parent participation mixed feed and 10 parts of ooze.
Preferably, step (2) are as follows: in the spawning season in Stichopus variegatus (Sempen) 5-9 month, select the front and back of the lunar calendar every month junior one
Induced spawning is carried out in 3 days, stopping bait throwing in 2 days before ovum is adopted in artificial stimulation, and that afternoon of ovum is adopted in stimulation, selects abdomen full
Healthy parent participation rinses ginseng body surface face well with filtering sea, is then put in airbreak in indoor raising of seedling pond and dries in the shade 40-60min, so
Filtering sea stimulation by running water 30-40min is used afterwards, seawater is reinjected, to Spawning；
Preferably, step (3) are as follows: after Spawning, parent participation is pulled out using nylon wire fishing net, excrement is removed in siphon
Just, core pulling and filling system then is made using 300 mesh sieve tulles, changes water 1-2 times, each quantity of exchanged water 50-70%, fertilized eggs are adopted in primary stimuli
Ovum is hatched in pond.
Preferably, step (4) are as follows: incubating oosperm develops after the first ear young, to collect the just ear young using siphonage, so
The first ear young is put in nursery pond afterwards and is cultivated, first ear young breeding density is 0.1-0.3/mL；With Chaetoceros muelleri, ball etc.
Whip chrysophyceae, spirulina powder, yeast are bait, and ten thousand cell/mL of Chaetoceros muelleri cell 1-2 are thrown in daily bait throwing in 2 times every time,
Isochrysis galbana cell thin 1000-2000 cell/mL, spirulina powder 0.02-0.12mg/L, yeast 0.02-0.12mg/L,
Bacillus 0.05-0.10mg/L；The young is put in a suitable place to breed the 1st to the 3rd day, daily the 9-11% sand filter seawater of addition seedling water, and the 4th
Water 20-30% is changed after it daily；It is doliolaria that the young is abnormal by the development of cultivation in 10-14 days；
It is further preferred that the young is put in a suitable place to breed the 1st to the 3rd day in step (4), 10% husky filter of seedling water is added daily
Seawater.
Preferably, step (5) are as follows: launch corrugated plating when the big ear larval metamorphosis of 10-20% is doliolaria and collect seedling, often put down
Square rice launches specification 40cm × 30cm corrugated plating 100-120 piece.During being finished after dispensing corrugated plating to doliolaria attachment, daily
Bait throwing in 2 times, 20,000 cell/mL of Chaetoceros muelleri cell, 2000 cell/mL of Isochrysis galbana, spirulina powder are thrown every time
0.12mg/L, yeast 0.12mg/L, bacillus 0.10mg/L；Stop changing water during collecting seedling, finishes, open to doliolaria attachment
Beginning flowing water improves water quality.
Preferably, step (6) are as follows: doliolaria settlement and metamorphosis be young ginseng after 10 days in, except feed spirulina, yeast and
Except bacillus, start to feed young ginseng mixed feed, sargassum thunbergii powder, sargassum powder and ooze, each daily ration, feeding quantity is spirulina
Powder 0.4-0.6g/m2, yeast 0.1-1.2g/m2, bacillus 0.1-1.2g/m2, young ginseng mixed feed 0.3-2.3g/m2, rat-tail
Algae powder 0.3-2.3g/m2, sargassum powder 0.3-2.3g/m2, ooze 1.0-3.0g/m2, morning and at dusk each throwing 1 time；Miniflow water with
It is cultivated under the conditions of continuous charge, day streamflow is 0.5-1.0 times of seedling water；Young Shenfu the rear 11st to the 40th day, stop throw spiral shell
Algae and yeast are revolved, each bait throwing in concentration is young ginseng mixed feed 2.5-5.0g/m2, sargassum thunbergii powder 2.5-5.0g/m2, tail algae powder
2.5-5.0g/m2, ooze 3.5-5.0g/m2, bacillus 0.2-1.0g/m2, morning and at dusk each throwing 1 time.Miniflow water and continuous
It is cultivated under venting condition, day streamflow is 1.5-2.0 times of seedling water, keeps dissolved oxygen 5mg/L or more.It is clear at interval of 5-7 days
Except bottom of pond residual bait and excrement 1 time；As young ginseng growth 1.0cm long up to body, children's ginseng breeding phase is initially entered.
Preferably, step (7) are as follows: doliolaria settlement and metamorphosis is about 40 days after young ginseng, seedling growth body is long reach 1cm with
Upper children's ginseng, joins the body long 1-8cm stage in children, and the young parameter amount of every square metre of cultivation is 0.2-0.5 ten thousand.Each bait throwing in concentration is children
Join mixed feed 5.5-8.0g/m2, sargassum thunbergii powder 5.5-8.0g/m2, tail algae powder 5.5-8.0g/m2, ooze 5.5-10.0g/m2,
Bacillus 1.0-1.2g/m2.Morning and at dusk each throwing 1 time.It is cultivated under the conditions of miniflow water and continuous charge, day streamflow is to educate
1.0-2.0 times of seedling water body keeps dissolved oxygen 5mg/L or more.At interval of 3-5 days removing bottom of pond residual baits and excrement 1 time；Flushing tank bottom
When, the children's ginseng gone out is collected with 20 mesh sieve tulles casees, and be moved back in nursery pond in time, avoid children's ginseng from overstocking injured and dead.
The present invention explore Stichopus variegatus (Sempen) induced spawning best opportunity and abductive approach and Stichopus variegatus (Sempen) auricularia, young ginseng,
The breeding method of children's ginseng, and optimize that Stichopus variegatus (Sempen) is young, the young ginseng process collection of falling pond tool, significantly improve young, young ginseng survival rate, this hair
Bright parent participation induced spawning success rate 80-100%, first ear larval culture planting percent 1.5-2.5%, one square metre of indoor raising of seedling pond training
2000 or so, the long 3-8cm seed of body is educated, has and solves the high technical problem of Stichopus variegatus (Sempen) nursery difficulty.
Specific embodiment
The present invention is described further referring to specific embodiment, to better understand the invention.
Embodiment 1
(1) parent participation is cultivated:
The natural Stichopus variegatus (Sempen) of weight 400-850g is selected, puts in a suitable place to breed and is cultivated in indoor cement pit or outdoor ponds, indoor pond
Breeding density 5-8 head/m2, outdoor ponds breeding density 1-2 head/m2, bottom of pond placement things concealed；Daily dusk bait throwing in 1 time, day throws
Bait amount is the 2-5% of parent participation weight, wherein in parts by weight, the nursing bait of parent participation includes sargassum thunbergii powder, sargassum
Powder, stichopus japonicus parent participation mixed feed and ooze etc. account for 25%, 25%, 40% and the 10% of daily ration, feeding quantity respectively.Indoor cement pit is cultivated
Continuous flowing water and inflation are kept, day streamflow is to cultivate 2-4 times of water body, periodically removes bottom of pond residual bait and excrement；Outdoor ponds every
It is changed water 1 time, and day quantity of exchanged water is the 10-20% of pond waters.Seawater salinity 26-33, dissolved oxygen content 5mg/L or more.
(2) ovum is adopted in artificial stimulation:
In the spawning season in Stichopus variegatus (Sempen) 5-9 month, the front and back of the lunar calendar every month junior one is selected to carry out artificial stimulation in 3 days
Ovum is adopted, stopping bait throwing in 2 days before ovum, that afternoon of induced spawning, the healthy parent participation for selecting abdomen full, with filtering sea are adopted in stimulation
Water rinses ginseng body surface face well, is then put in airbreak in indoor raising of seedling pond and dries in the shade 60min, then uses filtering sea stimulation by running water
30min reinjects seawater, to Spawning.Artificial stimulation adopts ovum success rate up to 100%.
(3) incubating oosperm:
After Spawning, parent participation is pulled out, excrement is removed in siphon, is made core pulling and filling system using 300 mesh sieve tulles, is changed water 1-
2 times, each quantity of exchanged water 70%, fertilized eggs are hatched in former induced spawning pond.
(4) larval culture:
Incubating oosperm develops after the first ear young, to collect the just ear young using siphonage, be then put in the first ear young
It is cultivated in nursery pond, first ear young breeding density is 0.1-0.3/mL.With Chaetoceros muelleri, Isochrysis galbana, spirulina powder,
Yeast is bait, daily bait throwing in 2 times, throws ten thousand cell/mL of Chaetoceros muelleri cell 1-2, Isochrysis galbana cell every time
Thin 1000-2000 cell/mL, spirulina powder 0.02-0.12mg/L, yeast 0.02-0.12mg/L, bacillus 0.05-
0.10mg/L；The young is put in a suitable place to breed the 1st to the 3rd day, adds 10% sand filter seawater of seedling water daily, changes water after the 4th day daily
20-30%；It is doliolaria that the young is abnormal by the development of cultivation in 10-14 days.First ear larval culture planting percent 1.5-2.5%.
(5) it collects seedling:
It launches corrugated plating when the big ear larval metamorphosis of 10-20% is doliolaria to collect seedling, every square metre of dispensing specification 40cm
× 30cm corrugated plating 100-120 piece.During finishing after dispensing corrugated plating to doliolaria attachment, daily bait throwing in 2 times throws try to gain every time
20,000 cell/mL of family name's Chaetoceros cell, Isochrysis galbana 2000 cell/mL, spirulina powder 0.12mg/L, yeast
0.12mg/L, bacillus 0.10mg/L.Stop changing water during collecting seedling, be finished to doliolaria attachment, starting flowing water improves water
Matter.
(6) young ginseng is cultivated:
Doliolaria settlement and metamorphosis be young ginseng after 10 days in, in addition to feeding spirulina, yeast and bacillus, start
Young ginseng mixed feed, sargassum thunbergii powder, sargassum powder and ooze are fed, each daily ration, feeding quantity is spirulina powder 0.4-0.6g/m2, yeast
0.1-1.2g/m2, bacillus 0.1-1.2g/m2, young ginseng mixed feed 0.3-2.3g/m2, sargassum thunbergii powder 0.3-2.3g/m2, horse
Tail algae powder 0.3-2.3g/m2, ooze 1.0-3.0g/m2, morning and at dusk each throwing 1 time；It is trained under the conditions of miniflow water and continuous charge
It educates, day streamflow is 0.5-1.0 times of seedling water,
Young Shenfu the rear 11st to the 40th day, stop throwing spirulina and yeast, each bait throwing in concentration is bacillus 0.2-
1.0g/m2, young ginseng mixed feed 2.5-5.0g/m2, sargassum thunbergii powder 2.5-5.0g/m2, tail algae powder 2.5-5.0g/m2, ooze 3.5-
5.0g/m2, morning and at dusk each throwing 1 time.It is cultivated under the conditions of miniflow water and continuous charge, day streamflow is seedling water 1.5-
2.0 times, keep dissolved oxygen 5mg/L or more.At interval of 5-7 days removing bottom of pond residual baits and excrement 1 time；When the growth of young ginseng is long up to body
When 1.0cm, children's ginseng breeding phase is initially entered.
(7) children's ginseng is cultivated:
Each bait throwing in concentration is children's ginseng mixed feed 5.5-8.0g/m2, sargassum thunbergii powder 5.5-8.0g/m2, tail algae powder 5.5-
8.0g/m2, ooze 5.5-10.0g/m2, bacillus 1.0-1.2g/m2, morning and at dusk each throwing 1 time.Miniflow water with continuously fill
It is cultivated under the conditions of gas, day streamflow is 1.0-2.0 times of seedling water, keeps dissolved oxygen 5mg/L or more.It was removed at interval of 3-5 days
Bottom of pond residual bait with excrement 1 time.About 40 days after doliolaria attachment, seedling growth body is long to reach 1cm or more children's ginseng, long in children's ginseng body
In the 1-8cm stage, the young parameter amount of every square metre of cultivation is 0.2-1.0 ten thousand, wherein one square metre of indoor raising of seedling pond long 3- of nurture body
2000,8cm seed or so.
Specific embodiments of the present invention are described in detail above, but it is merely an example, the present invention is simultaneously unlimited
It is formed on particular embodiments described above.To those skilled in the art, any couple of present invention carries out equivalent modifications and
Substitution is also all among scope of the invention.Therefore, without departing from the spirit and scope of the invention made by equal transformation and
Modification, all should be contained within the scope of the invention.
Claims (10)
1. a kind of mating system of Stichopus variegatus (Sempen) artificial seed, which comprises the following steps:
(1) parent participation is cultivated:
Fish natural Stichopus variegatus (Sempen), select ginseng body it is full, be parent participation without injured individual, put in a suitable place to breed in indoor cement pit or outdoor ponds
It cultivates, bottom of pond places things concealed；Using sargassum thunbergii powder, sargassum powder, stichopus japonicus parent participation mixed feed and ooze as bait, at dusk daily
Bait throwing in 1 time, daily ration, feeding quantity is the 2-3% of parent participation weight；Indoor cement pit, which is cultivated, keeps continuous flowing water and inflation, periodically removes bottom of pond
Residual bait and excrement；Outdoor ponds are changed water 1 time every other day；
(2) ovum is adopted in artificial stimulation:
In Stichopus variegatus (Sempen) spawning season, select to carry out artificial stimulation in the front and back 3 days of the lunar calendar every month junior one to adopt ovum；
(3) incubating oosperm:
After smart ovum is discharged in parent participation, parent participation is pulled out with the dab net that nylon wire makes, changes water with 300 mesh sieve tulles case siphons,
Change water 1-2 times, each quantity of exchanged water 50-70%, fertilized eggs are adopted in ovum pond in primary stimuli hatches；
(4) larval culture:
Then incubating oosperm development is put in the first ear young in nursery pond after the first ear young, to collect the just ear young and cultivates；With
Chaetoceros muelleri, Isochrysis galbana, spirulina powder and yeast are bait, daily bait throwing in 2 times；The young puts the 1st to the 3rd day in a suitable place to breed, often
The 9-11% sand filter seawater of its addition seedling water, changes water 20-30% daily after the 4th day；
(5) it collects seedling:
Polyethylene corrugated plate is launched when the big ear larval metamorphosis of 10-20% is doliolaria to collect seedling；To cup shape after dispensing corrugated plating
During young attachment finishes, using Chaetoceros muelleri, Isochrysis galbana, spirulina powder, yeast and bacillus as bait, throw daily
Bait 2 times；Stop changing water during collecting seedling, be finished to doliolaria attachment, starting flowing water improves water quality；
(6) young ginseng is cultivated:
Doliolaria settlement and metamorphosis be young ginseng after 10 days in, in addition to feeding spirulina, yeast and bacillus, start to feed
Young ginseng mixed feed, sargassum thunbergii powder, sargassum powder and ooze, morning and dusk respectively throw 1 time, under the conditions of miniflow water and continuous charge
It cultivates；Young Shenfu the rear 11st to the 40th day, stop throwing spirulina and yeast, morning and each at dusk throw 1 time；Miniflow water with continuously fill
It is cultivated under the conditions of gas；At interval of 5-7 days removing bottom of pond residual baits and excrement 1 time, young ginseng is collected using 40 mesh sieve tulles casees, and in time
The young ginseng of collection is moved on in nursery pond；As young ginseng growth 1.0cm long up to body, children's ginseng breeding phase is initially entered；
(7) children's ginseng is cultivated:
Using children's ginseng mixed feed, sargassum thunbergii powder, sargassum powder, ooze and bacillus as bait, morning and dusk are respectively thrown 1 time；
It is cultivated under the conditions of miniflow water and continuous charge；At interval of 3-5 days removing bottom of pond residual baits and excrement 1 time, using 20 mesh sieve tulles casees
Collect children's ginseng；Culture about 40 days.
2. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (1) are as follows: selective body
The weight natural Stichopus variegatus (Sempen) of 400-850g, puts in a suitable place to breed and cultivates in indoor cement pit or outdoor ponds, and indoor pond breeding density 5-8 head/
m2, outdoor ponds breeding density 1-2 head/m2, bottom of pond placement things concealed；Daily dusk bait throwing in 1 time, daily ration of feeding is parent participation weight
2-3%, wherein in parts by weight, the nursing bait of parent participation includes 22-27 parts of sargassum thunbergii powder, sargassum powder 22-27
Part, 38-42 parts of stichopus japonicus parent participation mixed feed and 8-12 parts of ooze；Indoor cement pit, which is cultivated, keeps continuous flowing water and inflation, flows day
Water is to cultivate 2-4 times of water body, periodically removes bottom of pond residual bait and excrement；Outdoor ponds are changed water 1 time every other day, and day quantity of exchanged water is pond
The 10-20% of water body；Seawater salinity 26-33, dissolved oxygen content 5mg/L or more.
3. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 2, which is characterized in that in step (1), according to weight
Number meter is measured, the bait that feeds of parent participation includes 25 parts of sargassum thunbergii powder, 25 parts of sargassum powder, 40 parts of stichopus japonicus parent participation mixed feed and sea
10 parts of mud.
4. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (2) are as follows: pierced in flower
In the spawning season for joining 5-9 month, the front and back of the lunar calendar every month junior one is selected to carry out induced spawning in 3 days, ovum is adopted in artificial stimulation
That afternoon of ovum is adopted in stopping bait throwing in first 2 days, stimulation, the healthy parent participation for selecting abdomen full, with filtering sea ginseng body surface face
It rinses well, is then put in airbreak in indoor raising of seedling pond and dries in the shade 40-60min, then use filtering sea stimulation by running water 30-40min,
Seawater is reinjected, to Spawning.
5. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (3) are as follows: smart ovum row
After putting, parent participation is pulled out using nylon wire fishing net, excrement is removed in siphon；Then core pulling and filling system is made using 300 mesh sieve tulles,
Change water 1-2 times, each quantity of exchanged water 50-70%, fertilized eggs are adopted in ovum pond in primary stimuli hatches.
6. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (4) are as follows: fertilized eggs
Hatching develops after the first ear young, to collect the just ear young using siphonage, be then put in nursery pond the first ear young and cultivate, just
Ear young breeding density is 0.1-0.3/mL；Using Chaetoceros muelleri, Isochrysis galbana, spirulina powder, yeast as bait, daily
Bait throwing in 2 times, ten thousand cell/mL of Chaetoceros muelleri cell 1-2 are thrown every time, it is thin 1000-2000, Isochrysis galbana cell thin
Born of the same parents/mL, spirulina powder 0.02-0.12mg/L, yeast 0.02-0.12mg/L, bacillus 0.05-0.10mg/L；The young is put in a suitable place to breed
1st to the 3rd day, the 9-11% sand filter seawater of seedling water was added daily, changes water 20-30% after the 4th day daily；The young passes through
The abnormal development of cultivation in 10-14 days is doliolaria.
7. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 6, which is characterized in that in step (4), the young is put
It supports the 1st to the 3rd day, adds 10% sand filter seawater of seedling water daily.
8. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (5) are as follows: work as 10-
It launches corrugated plating when 20% big ear larval metamorphosis is doliolaria to collect seedling, every square metre of dispensing specification 40cm × 30cm corrugated plating
100-120 piece.During finishing after dispensing corrugated plating to doliolaria attachment, it is a thin to throw Chaetoceros muelleri every time for daily bait throwing in 2 times
20,000 cell/mL of born of the same parents, Isochrysis galbana 2000 cell/mL, spirulina powder 0.12mg/L, yeast 0.12mg/L, bacillus
0.10mg/L；Stop changing water during collecting seedling, be finished to doliolaria attachment, starting flowing water improves water quality.
9. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (6) are as follows: cup shape children
Body settlement and metamorphosis is in addition to feeding spirulina, yeast and bacillus, to start to feed young ginseng cooperation feeding in 10 days after young ginseng
Material, sargassum thunbergii powder, sargassum powder and ooze, each daily ration, feeding quantity are spirulina powder 0.4-0.6g/m2, yeast 0.1-1.2g/m2, bud
Spore bacillus 0.1-1.2g/m2, young ginseng mixed feed 0.3-2.3g/m2, sargassum thunbergii powder 0.3-2.3g/m2, sargassum powder 0.3-
2.3g/m2, ooze 1.0-3.0g/m2, morning and at dusk each throwing 1 time；Cultivated under the conditions of miniflow water and continuous charge, day streamflow
It is 0.5-1.0 times of seedling water；Young Shenfu the rear 11st to the 40th day, stop throwing spirulina and yeast, each bait throwing in concentration is young
Join mixed feed 2.5-5.0g/m2, sargassum thunbergii powder 2.5-5.0g/m2, tail algae powder 2.5-5.0g/m2, ooze 3.5-5.0g/m2, bud
Spore bacillus 0.2-1.0g/m2, morning and at dusk each throwing 1 time；It is cultivated under the conditions of miniflow water and continuous charge, day streamflow is nursery
1.5-2.0 times of water body, keep dissolved oxygen 5mg/L or more；At interval of 5-7 days removing bottom of pond residual baits and excrement 1 time；When young ginseng is grown
When 1.0cm long up to body, children's ginseng breeding phase is initially entered.
10. the mating system of Stichopus variegatus (Sempen) artificial seed according to claim 1, which is characterized in that step (7) are as follows: cup shape
About 40 days after the young ginseng of young settlement and metamorphosis, seedling growth body is long to reach 1cm or more children's ginseng, joins the body long 1-8cm stage in children, often puts down
Square meter Pei Yu children's parameter amount is 0.2-0.5 ten thousand；Each bait throwing in concentration is children's ginseng mixed feed 5.5-8.0g/m2, sargassum thunbergii powder
5.5-8.0g/m2, tail algae powder 5.5-8.0g/m2, ooze 5.5-10.0g/m2, bacillus 1.0-1.2g/m2.Morning and dusk
It is each to throw 1 time；It is cultivated under the conditions of miniflow water and continuous charge, day streamflow is 1.0-2.0 times of seedling water, keeps dissolved oxygen 5mg/
L or more；At interval of 3-5 days removing bottom of pond residual baits and excrement 1 time；When flushing tank bottom, the children gone out is collected with 20 mesh sieve tulles casees
Ginseng, and be moved back in nursery pond in time, avoid young ginseng from overstocking injury and death.
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| CN111869608A (en) * | 2020-08-06 | 2020-11-03 | 大连棒棰岛海参发展有限公司 | Ecological breeding method for breeding stichopus japonicus young stichopus japonicus fries in small net cages of pond |
| CN112655609A (en) * | 2020-12-18 | 2021-04-16 | 广东省农业科学院动物科学研究所 | Method for industrially culturing tropical pink wart sea cucumber seedlings |
| CN112655611A (en) * | 2020-12-18 | 2021-04-16 | 广东省农业科学院动物科学研究所 | Method for reversely hanging, dispersedly domesticating and hastening parents of Apostichopus japonicus selenka, hastening parturition and efficiently cultivating seedlings |
| CN112655610A (en) * | 2020-12-18 | 2021-04-16 | 广东省农业科学院动物科学研究所 | Efficient cultivation method for tropical sea cucumber seedlings |
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