CN109628342A - A kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation and application - Google Patents
A kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation and application Download PDFInfo
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- CN109628342A CN109628342A CN201811596742.XA CN201811596742A CN109628342A CN 109628342 A CN109628342 A CN 109628342A CN 201811596742 A CN201811596742 A CN 201811596742A CN 109628342 A CN109628342 A CN 109628342A
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- bacillus subtilis
- bacillus licheniformis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/06—Coating or dressing seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- Health & Medical Sciences (AREA)
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Abstract
A kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation and application, the invention belongs to microbial manure technical fields.Said preparation includes bacillus subtilis and bacillus licheniformis, and said preparation can promote plant growth and take root.Bacillus subtilis, bacillus licheniformis promoting growth of plants preparation are for soaking seed, dressing seed, nursery, cuttage or forest are transplanted.Said preparation is compared with commercially available increasing agent, volume increase 5% or so；Compared with blank, 12% or more volume increase；Volume increase is achieved the effect that.
Description
Technical field
The invention belongs to microbial manure technical fields, the in particular to microbial inoculum of bacillus subtilis, bacillus licheniformis
And preparation method thereof and the microbial inoculum that is prepared by bacillus subtilis, bacillus licheniformis promoting plant growth and the side of taking root
The application in face.
Background technique
For a long time, chemical fertilizer is largely applied in agricultural production, although chemical fertilizer fertilizer efficiency is more apparent, due to changing
Inorganic nutrients contained by fertile the inside are easy the ingredients such as the lime contained in soil and fix, and lead to soil hardening, microorganism disappears
It loses, soil fertility decline makes the harmful chemicals residue that Soil accumulation is numerous, and pest and disease damage is rampant, and the application of a large amount of chemical fertilizer can also be right
Water source causes seriously to pollute, and agroecological environment and environment for human survival is caused to be seriously threatened.At the same time, excessive
Chemical fertilizer also will have a direct impact on quality of agricultural product and yield, reduce the market competitiveness of agricultural product.
While chemical fertilizer brings some negative effects, microbial manure is increasingly subject to the concern of people, in modern agriculture
Sustainable development in occupy increasingly important role and status.Microbial manure is living body fertilizer, containing a large amount of beneficial to micro-
Biology, after being manured into soil, largely microorganism living under optimum conditions can be actively movable, can activate because chemistry is used for a long time
Soil hardening caused by fertilizer and remaining n-p-k element, increase soil organic matter content, and secretion growth hormone stimulates crop
Growth promotes root system development, improves the quality of crops, improves crop drought resistance, disease resistance, increases income and lays for crop yield
Good basis.
Summary of the invention
It is an object of the present invention to provide the microorganism formulations of a kind of nontoxic, pollution-free, noresidue and safety to promote plant raw
Grow, take root, survive and improve crop yield.
Technical solution of the invention a kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation as follows,
In total strain number ratio in the said preparation microbial inoculum are as follows: bacillus subtilis 80%, bacillus licheniformis 20%.
The purchase of the strain of bacillus subtilis and bacillus licheniformis is single in the industrial Bioisystech Co., Ltd in Hebei Cangzhou
Bit address: Hebei province, Hebei Cangzhou, Cang County industry park Xing Jizhen, telephone number: 15200830432.
The preparation method of said preparation the following steps are included:
, after (1) activates bacillus subtilis, bacillus licheniformis, by 5~10% inoculum concentrations, by the bacillus subtilis of activation
Bacterium, bacillus licheniformis are inoculated into seed culture medium, under the conditions of 35 DEG C of cultivation temperature, revolving speed 120rpm/min, shaking table culture
36~48h, obtains primary seed solution, and the seed culture medium is LB liquid medium, pH7.0~7.2；
(2) 10~12% inoculum concentrations are pressed, primary seed solution are transferred to the secondary seed medium in seeding tank, 28~37
DEG C, revolving speed 100rpm/min, ventilatory capacity 1000L/h, tank pressure cultivate 36~48h under the conditions of being maintained at 0.02~0.03MPa,
Obtain secondary seed solution；The secondary seed medium is made of following component based on mass fraction: starch 1.5~3%,
Wheatfeed 1~2%, yeast extract 0.05~0.3%, peptone 0.5~1%, ground phosphate rock 1~2%, MgSO4 7H2O0.01~
0.05%, CaCl20.1~0.4%, (NH4) 2SO40.01~0.04%, surplus are water, pH7.0~7.2；
(3) 10~12% inoculum concentrations are pressed, secondary seed solution is inoculated into the fermentation medium in fermentor, at 28~37 DEG C, are turned
Fast 100rpm/min, ventilatory capacity 10000L/h, tank pressure cultivate 48~72h, culture under the conditions of being maintained at 0.02~0.03MPa
You effect Shuo≤2 × 10 Jun living of bacillus subtilis, bacillus licheniformis in fermentation liquid afterwards8When a/m, fermentation liquid is cured
With plastic bottle packing, the as described promoting growth of plants preparation；The fermentation medium and step (2) described secondary seed culture
Base phase is same.
Said preparation is for soaking seed, dressing seed, nursery, cuttage or forest are transplanted.
The invention has the benefit that said preparation all realizes volume increase 5% or so compared with commercially available FZB42, compared with blank
10% or more volume increase.Tomato test result shows: the tomato plant height, stem thickness, fruit mass and yield of this product and FZB42 processing
It is all significant or it is extremely significant be higher than control, 500 times of liquid cell productions of this product than control rate of growths up to 17.34%, this product 500
The 500 times of liquid volume increase 5.48% of times liquor ratio FZB42 suspending agent.
Cucumber test the result shows that: this product and cucumber plant height, stem thickness, fruit mass and the yield of FZB42 processing are all aobvious
It writes or extremely significant higher than control.Compared with the control, 500 times of liquid cell production rate of growths of this product are 12.53%, more outstanding than FZB42
500 times of liquid volume increase 5.24% of floating agent.
Specific embodiment
It is further illustrated below with reference to embodiment, each embodiment is conventional method without specified otherwise.Described in following embodiment
FZB42 suspending agent be it is commercially available, be a kind of microbial manure product containing bacillus amyloliquefaciens..
The preparation of the promoting growth of plants preparation of the present invention of embodiment 1
Preparation method the following steps are included:
(1) prepared by primary seed solution
The bacillus subtilis of -80 DEG C of preservations, bacillus licheniformis are seeded on LB liquid medium, 35 DEG C of cultures for 24 hours, obtain
Bacillus subtilis, the bacillus licheniformis of activation, by 10% inoculum concentration, by the bacillus subtilis of activation, lichens gemma bar
Bacterium is inoculated into seed culture medium, and under the conditions of 35 DEG C of cultivation temperature, revolving speed 120rpm/min, shaking table culture 36h obtains level-one kind
Sub- liquid, the seed culture medium are LB liquid medium, pH7.0~7.2；
(2) 10% inoculum concentration is pressed, primary seed solution is transferred to the secondary seed medium in seeding tank, at 35 DEG C, revolving speed
100rpm/min, ventilatory capacity 1000L/h, tank pressure cultivate 36~48h under the conditions of being maintained at 0.02~0.03MPa, obtain second level
Seed liquor；The secondary seed medium is made of following component based on mass fraction: starch 2%, wheatfeed 1%, yeast
Cream 0.3%, peptone 0.5%, ground phosphate rock 1%, MgSO4 7H2O 0.02%, CaCl20.25%, (NH4) 2SO40.03%,
Surplus is water, and pH7.0~7.2, the secondary seed medium is in 121 DEG C of sterilizing 30min, wait be cooled to 30 DEG C, access level-one
Seed liquor.
(3) 10% inoculum concentration is pressed, secondary seed solution is inoculated into the fermentation medium in fermentor, at 35 DEG C, revolving speed
100rpm/min, ventilatory capacity 10000L/h, tank pressure cultivate 48~72h under the conditions of being maintained at 0.02~0.03MPa, after culture
Fermentation liquid in bacillus subtilis, bacillus licheniformis living bacteria count be 2 × 108When a/ml, by fermentation liquid with medical
Plastic bottle packing, the as described promoting growth of plants preparation, the fermentation medium and step (2) the secondary seed medium phase
Together.
Promoting growth of plants preparation of the present invention is prepared by the preparation method of promoting growth of plants preparation as described in example 2.
The promoting growth of plants preparation of the present invention of embodiment 2 is promoting the application on plant growth
Promoting growth of plants preparation of the present invention is hereinafter referred to as: this product.
One, overall operation
1, it soaks seed
1. impregnating: being impregnated crop seed 10~40 minutes with 500~1000 times of dilutions of this product, pull seed out, make after drying in the shade
With.
2, it dresses seed
Before sowing, with 500~1000 times of dilution seed dressings of this product, then dry, this product dilution dosage is grain weight
1/50。
3, nursery
1. after planting at once: being poured using 500~1000 times of dilutions of this product, later every 7-10 days, apply 1 time, share
3-4 times；
2. after plantation: the seedling after transplanting is poured using 500~1000 times of dilutions of this product immediately, later every 7-10 days,
Application 1 time shares 3-4 times.
4, cuttage
1. nursery stock cuttage: woody flower (such as camellia, azalea, rose, crape myrtle, Lindley Butterflybush Herb) and commodity trees (such as red bean
China fir, podocarpus, China fir, Dendrocalamus brandisii etc.) branch be cut into the segment with 1-2 leaf bud, in 200 times of dilutions of this product
After impregnating 20-30 minutes in middle immersion 5-10 minutes or 500 times of dilutions, cuttage then pours this product 500 on seedbed
Times dilution is applied 1 time later every 7-10 days, is shared 3 times.
2. forest is transplanted: when large-scale commodity trees (such as China fir, Taiwania flousiana, alder) balled transplanting, with this product 500-
1000 times of dilution liquid irrigating roots water the soil of tree base portion with the bacterium solution before earthing a little, after earthing, then with the bacterium of same concentration
Liquid irrigates, and pours within 5-7 days once, shares 3 times again.
3. beating mud: mud is broken into 100-500 times of dilution of this product, can be used for plant rooting of cuttings, it can also be in naked
The transplanting of root sapling uses, and sapling root is put into the mud before transplanting, after being stained with the mud, directly transplants.
Application of the embodiment 3 as fertilizer
1) it applies on vegetables
A, this product pouring root application on tomato, cucumber
Field growth-promoting effect of this product on tomato, cucumber
Control is FZB42 suspending agent, carries out growth promotion on tomato (kind is green prosperous 108 gold medal cup), cucumber (kind is winter hat)
Comparative test.
Tomato and cucumber test carry out in the heliogreenhouse of Daliang City, Liaoning Province Chenggong Vegetable Base.Greenhouse is steelframe
Vinyl house (long X wide=63mx9m, 567m2), experimental field soil is brown earth, in fertility etc..3 processing are set altogether:
Processing 1: 500 times of liquid of this product (concentration is 2.0 × 105cfu/mL).
Handle 500 times of liquid of 2:FZB42 suspending agent (concentration is 2.0 × 105cfu/mL).
Processing 3: equivalent clear water compares (CK).
3 repetitions of each processing, random alignment.Plot area is 8.4m (1.2mx7m).Ridging plastic-film-covered cultivation, spacing in the rows
33cm, small line-spacing 40cm, wide row space 80cm.
Influence of this product to tomato, cucumber growth character and yield
Tomato test result shows: this product and tomato plant height, stem thickness, fruit mass and the yield of FZB42 processing it is all significant or
It is extremely significant to be higher than control, 500 times of liquid cell productions of this product than control rate of growths up to 17.34%, 500 times of liquor ratios of this product
The 500 times of liquid volume increase 5.48% of FZB42 suspending agent.
Cucumber test the result shows that: this product and cucumber plant height, stem thickness, fruit mass and the yield of FZB42 processing are all aobvious
It writes or extremely significant higher than control.Compared with the control, 500 times of liquid cell production rate of growths of this product are 12.53%, more outstanding than FZB42
500 times of liquid volume increase 5.24% of floating agent.
Claims (3)
1. a kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation, it is characterised in that: pressed in the said preparation microbial inoculum
Total strain number ratio are as follows: bacillus subtilis 80%, bacillus licheniformis 20%.
2. the preparation side of a kind of bacillus subtilis according to claim 1, bacillus licheniformis promoting growth of plants preparation
Method, which is characterized in that comprise the steps of:
(1) after activating bacillus subtilis, bacillus licheniformis, by 5~10% inoculum concentrations, by the bacillus subtilis of activation
Bacterium, bacillus licheniformis are inoculated into seed culture medium, under the conditions of 35 DEG C of cultivation temperature, revolving speed 120rpm/min, shaking table culture
36~48h, obtains primary seed solution, and the seed culture medium is LB liquid medium, pH7.0~7.2；
(2) 10~12% inoculum concentrations are pressed, primary seed solution are transferred to the secondary seed medium in seeding tank, 28~37
DEG C, revolving speed 100rpm/min, ventilatory capacity 1000L/h, tank pressure cultivate 36~48h under the conditions of being maintained at 0.02~0.03MPa,
Obtain secondary seed solution；The secondary seed medium is made of following component based on mass fraction: starch 1.5~3%, bran
Skin powder 1~2%, yeast extract 0.05~0.3%, peptone 0.5~1%, ground phosphate rock 1~2%, MgSO4•7H2O 0.01~
0.05%, CaCl20.1~0.4%, (NH4)2SO40.01~0.04%, surplus is water, pH7.0~7.2；
(3) 10~12% inoculum concentrations are pressed, secondary seed solution is inoculated into the fermentation medium in fermentor, at 28~37 DEG C, are turned
Fast 100rpm/min, ventilatory capacity 10000L/h, tank pressure cultivate 48~72h, culture under the conditions of being maintained at 0.02~0.03MPa
You effect Shuo≤2 × 10 Jun living of bacillus subtilis, bacillus licheniformis in fermentation liquid afterwards8When a/ml, obtain promoting plant life
Long preparation；The fermentation medium is identical as step (2) secondary seed medium.
3. the application of a kind of bacillus subtilis according to claim 1, bacillus licheniformis promoting growth of plants preparation,
Be characterized in that: said preparation is applied to seed soaking, seed dressing, nursery, cuttage or forest transplanting.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811596742.XA CN109628342A (en) | 2018-12-26 | 2018-12-26 | A kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation and application |
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| CN201811596742.XA CN109628342A (en) | 2018-12-26 | 2018-12-26 | A kind of bacillus subtilis, bacillus licheniformis promoting growth of plants preparation and application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142018A (en) * | 2021-04-30 | 2021-07-23 | 上海欧思景观设计工程有限公司 | Podocarpus macrophyllus planting soil |
| CN113383652A (en) * | 2021-07-19 | 2021-09-14 | 石家庄市农林科学研究院 | Technical method for inducing lateral roots of alpine rhododendron |
| CN117356221A (en) * | 2023-11-23 | 2024-01-09 | 西藏自治区农牧科学院草业科学研究所 | Method for improving germination of Phaffia scirpa seeds by utilizing soil-promoting bacteria |
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| CN102786934A (en) * | 2012-08-22 | 2012-11-21 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN103789235A (en) * | 2014-01-19 | 2014-05-14 | 王金玲 | Successive planting resistance compound microbial agent and production method thereof |
| CN105331567A (en) * | 2015-12-21 | 2016-02-17 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens, plant growth promoter and application of plant growth promoter |
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| CN102786934A (en) * | 2012-08-22 | 2012-11-21 | 王金玲 | Probiotic soil conditioner and production method thereof |
| CN103789235A (en) * | 2014-01-19 | 2014-05-14 | 王金玲 | Successive planting resistance compound microbial agent and production method thereof |
| CN105331567A (en) * | 2015-12-21 | 2016-02-17 | 云南省微生物发酵工程研究中心有限公司 | Bacillus amyloliquefaciens, plant growth promoter and application of plant growth promoter |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113142018A (en) * | 2021-04-30 | 2021-07-23 | 上海欧思景观设计工程有限公司 | Podocarpus macrophyllus planting soil |
| CN113383652A (en) * | 2021-07-19 | 2021-09-14 | 石家庄市农林科学研究院 | Technical method for inducing lateral roots of alpine rhododendron |
| CN117356221A (en) * | 2023-11-23 | 2024-01-09 | 西藏自治区农牧科学院草业科学研究所 | Method for improving germination of Phaffia scirpa seeds by utilizing soil-promoting bacteria |
| CN117356221B (en) * | 2023-11-23 | 2024-04-19 | 西藏自治区农牧科学院草业科学研究所 | Method for improving germination of Phaffia scirpa seeds by utilizing soil-promoting bacteria |
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