CN109607601A - A kind of promotion seed sprouting and the nano material taken root and preparation method thereof - Google Patents

A kind of promotion seed sprouting and the nano material taken root and preparation method thereof Download PDF

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CN109607601A
CN109607601A CN201910047604.4A CN201910047604A CN109607601A CN 109607601 A CN109607601 A CN 109607601A CN 201910047604 A CN201910047604 A CN 201910047604A CN 109607601 A CN109607601 A CN 109607601A
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solution
znsn
seed
root
nano material
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师进生
陈蔚燕
张原原
王丽丽
孔晓颖
兰学芳
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Qingdao Agricultural University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01G—COMPOUNDS CONTAINING METALS NOT COVERED BY SUBCLASSES C01D OR C01F
    • C01G19/00—Compounds of tin
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/01—Particle morphology depicted by an image
    • C01P2004/04—Particle morphology depicted by an image obtained by TEM, STEM, STM or AFM
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/30—Particle morphology extending in three dimensions
    • C01P2004/32—Spheres
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01P—INDEXING SCHEME RELATING TO STRUCTURAL AND PHYSICAL ASPECTS OF SOLID INORGANIC COMPOUNDS
    • C01P2004/00—Particle morphology
    • C01P2004/30—Particle morphology extending in three dimensions
    • C01P2004/32—Spheres
    • C01P2004/34—Spheres hollow

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  • Condensed Matter Physics & Semiconductors (AREA)
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  • General Physics & Mathematics (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses a kind of sprouting of promotion seed and the nano material taken root and preparation method thereof, there is the ability for promoting seed quick-speed germination and raising seed to take root.The nano material is a kind of nanometer ZnS n (OH)6Material, structure are core-shell structure.Pass through a large amount of indoor and field trial, nanometer ZnS n (OH)6Material has the effect of significant growth-promoting root and seed is promoted to sprout, and such as takes root for wheat, root long, radical can be improved 14% or so；88.3% can be improved to the germination of wheat seed, higher than the germination percentage of concurrent control group.Low with production and use cost, dosage is small, wide using concentration range, safe and convenient to use, can be widely used for various crops or plant promoting root growth, volume increase.

Description

A kind of promotion seed sprouting and the nano material taken root and preparation method thereof
Technical field
The present invention relates to crop planting technical fields, and in particular to the Cotton seeds method of crop seeds.
Background technique
Application report of the nanotechnology on insecticide, fungicide it is very much, it has been found that, nano-sized carbon to rice have promote Into effect, nano-sized carbon has production-increasing function, carbon nanomaterial such as MWCNTs, OMWCNTs, SWCNHs, performance to soybean as fertilizer To promote seed to the absorption of moisture, the sprouting of seed can be accelerated, the germination percentage of seed can be improved.Nano-TiO2To oil The germination percentage of dish, cucumber and corn has inhibiting effect, and it is also highly significant to the inhibiting effect of corn germination rate. ZnO nano particle and gold nano grain generate inhibiting effect to corn seed germination rate.MWCNT to wheat, beans, peanut kind Son germination all has raising, and research shows that nano material can promote seed to sprout to varying degrees.Also studies have reported that, Nano-sized carbon can promote absorption of the corn to nitrogen.Carbon nanohorn SWCNHs makes fresh tobacco bring up again height.Nanometer Fe particle, nano-ZnO Particle, nanometer CuO particle etc. study the effect that inhibition is had to maize seedling root.Single-walled carbon nanotube, multi-walled carbon nanotube, Influence of the carbon nanotube to plant growth and development be it is complicated and changeable, this depends primarily on the type of carbon nanotube.
Summary of the invention
The object of the present invention is to provide a kind of with promotion seed quick-speed germination and improves the novel of seed rootability Nano material is specifically to provide a kind of nanometer ZnS n (OH)6For material as seed promotor, it can be used for agricultural or forestry Volume increase or survival rate improve.
To achieve the above object, the present invention the following technical schemes are provided:
Step 1, by SnCl4·5H2O is added in dehydrated alcohol and stirs, and forms solution 1；
Step 2, by Zn (Ac)2·2H2O and SDBS (neopelex) are added in distilled water, and stirring forms solution 2；
Step 3 according to the volume ratio of solution 1 and solution 2 is 1:2, and solution 1 is stirred at room temperature with solution 2 and is mixed, drop Adding sodium hydroxide solution；
Step 4, by solution obtained in the previous step, precipitating is collected by centrifugation, is respectively washed three times with water and ethyl alcohol, 60 DEG C are dry The ZnSn (OH) of solid construction6。
Step 5, the ZnSn (OH) by solid construction6As presoma, Zn (Ac)2·2H2O and SDBS are added in distilled water, Stirring forms solution 3；
Step 6, by SnCl4·5H2O is added to stirring in dehydrated alcohol and forms solution 4；
Mixing is stirred at room temperature in solution 3 and solution 4 by step 7, and sodium hydroxide solution is added dropwise into mixed liquor；
Step 8, by solution obtained in the previous step, white precipitate is collected by centrifugation, is respectively washed three times with water and ethyl alcohol, 60 DEG C are dry The dry ZnSn (OH) for obtaining core-shell structure6。
SnCl in the solution 1 that the step 1 is prepared4·5H2The molar concentration of O solution is 0.0005~0.0014mol/L, Mixing time is 10min.
Zn (Ac) in the solution 2 that the step 2 is prepared2·2H2The molar concentration of O solution is 0.0005~0.0015mol/ The molar concentration of L, SDBS are 0.0005~0.0014mol/L, mixing time 10min.
In the step 3 be added dropwise sodium hydroxide molar concentration be 2mol/L, the volume of the sodium hydroxide solution of dropwise addition with The volume ratio for the mixed solution that solution 1, solution 2 are formed is 1:3.
ZnSn (OH) in step 5 solution 36Molar concentration be 0.0001~0.0003mol/L, Zn (Ac)2·2H2O Molar concentration be 0.0005~0.0015mol/L, the molar concentration of SDBS is 0.0005~0.0014mol/L, mixing time For 10min.
SnCl in step 6 solution 44·5H2The molar concentration of O solution is 0.0005~0.0014mol/L, stirring Time is 10min.
In the step 7 be added dropwise sodium hydroxide molar concentration be 2mol/L, the volume of the sodium hydroxide solution of dropwise addition with The volume ratio for the mixed solution that solution 3, solution 4 are formed is 1:3.
Preferably, step 1, SnCl described in step 64·5H2The concentration of O solution is 0.001mol/L；Step 2, step Zn described in 5 (Ac)2·2H2The concentration of O is 0.001mol/L, and the concentration of SDBS is 0.001mol/L；ZnSn described in step 5 (OH)6Concentration be 0.00017mol/L.
The advantages and positive effects of the present invention:
1. by a large amount of indoor and field trial, nanometer ZnS n (OH)6Material has significant growth-promoting root and promotes kind The effect that son is sprouted, such as takes root for wheat, root long, radical can be improved 14% or so；The germination of wheat seed can be improved 88.3%, higher than the germination percentage of same period blank control group.
2. nanometer ZnS n (OH) of the invention6Material has production and use cost low, and dosage is small, uses concentration range Width, it is safe and convenient to use, it can be widely used for various crops or plant promoting root growth, volume increase etc..It should be clear that in power of the invention Benefit requires in limited range, can carry out various transformation and change.
Detailed description of the invention
With reference to the accompanying drawing and embodiment the present invention is described in further detail:
Fig. 1: the nanometer ZnS n (OH) of solid construction6Transmission electron microscope picture, what " (a) ZSK solid " was represented in figure is solid The nanometer ZnS n (OH) of structure6。
Fig. 2: the nanometer ZnS n (OH) of core-shell structure6Transmission electron microscope picture, what " (b) ZSK yolk-shell " was represented in figure is The nanometer ZnS n (OH) of core-shell structure6, it is nanometer ZnS n (OH) pointed by " shell "6Shell, be pointed by " yolk " The solid nanometer ZnS n (OH) that dotted line surrounds6Material.
Fig. 3: control group and influence of core-shell structure ZnSn (OH) 6 material to wheat seed germinating are compared by seed soaking mode Comparison diagram, left side is the case where control group wheat takes root and germinate in figure, and right side is core-shell nano ZnSn (OH)6After material processing Wheat the case where taking root and germinateing.
Specific embodiment
Following synthesis example, formulation examples and raw experimental result of surveying can be used to further illustrate the present invention, but not mean that The limitation present invention.
Embodiment 1:
Step 1: by the SnCl of 0.2g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 1；
Step 2: by the Zn (Ac) of 0.11g2·2H2The SDBS of O and 0.2g is added in 10mL water, stirs 10min, is formed molten Liquid 2；
It is mixed Step 3: solution 1 is stirred at room temperature with solution 2, the sodium hydroxide solution of 5mL 2moL/L is added dropwise；
Step 4: precipitating is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C of dryings Obtain the ZnSn (OH) of solid construction6。
Step 5: taking the ZnSn (OH) of 28mg solid construction6As presoma, 0.11gZn (Ac)2·2H2O's and 0.2g SDBS (neopelex) is added in 10mL water, stirs 10min, forms solution 3；
Step 6: taking the SnCl of 0.2g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 4；
Step 7: mixing is stirred at room temperature in solution 3 and solution 4, take the sodium hydroxide of 5mL 2mol/L that mixing is added In liquid；
Step 8: white precipitate is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C The ZnSn (OH) of dry core-shell structure6。
Embodiment 2:
Step 1: by the SnCl of 0.5g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 1；
Step 2: by the Zn (Ac) of 0.11g2·2H2The SDBS of O and 0.2g is added in 10mL water, stirs 10min, is formed molten Liquid 2；
It is mixed Step 3: solution 1 is stirred at room temperature with solution 2, the sodium hydroxide solution of 5mL 2moL/L is added dropwise；
Step 4: precipitating is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C of dryings Obtain the ZnSn (OH) of solid construction6。
Step 5: taking the ZnSn (OH) of 85mg solid construction6As presoma, 0.11g Zn (Ac)2·2H2O's and 0.2g SDBS (neopelex) is added in 10mL water, stirs 10min, forms solution 3；
Step 6: taking the SnCl of 0.5g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 4；
Step 7: mixing is stirred at room temperature in solution 3 and solution 4, take the sodium hydroxide of 5mL 2mol/L that mixing is added In liquid；
Step 8: white precipitate is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C The ZnSn (OH) of dry core-shell structure6。
Embodiment 3:
Step 1: by the SnCl of 0.2g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 1；
Step 2: by the Zn (Ac) of 0.33g2·2H2The SDBS of O and 0.5g is added in 10mL water, stirs 10min, is formed molten Liquid 2；
It is mixed Step 3: solution 1 is stirred at room temperature with solution 2, the sodium hydroxide solution of 5mL 2moL/L is added dropwise；
Step 4: precipitating is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C of dryings Obtain the ZnSn (OH) of solid construction6。
Step 5: taking the ZnSn (OH) of 85mg solid construction6As presoma, 0.33gZn (Ac)2·2H2O's and 0.5g SDBS (neopelex) is added in 10mL water, stirs 10min, forms solution 3；
Step 6: taking the SnCl of 0.2g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 4；
Step 7: mixing is stirred at room temperature in solution 3 and solution 4, take the sodium hydroxide of 5mL 2mol/L that mixing is added In liquid；
Step 8: white precipitate is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C The ZnSn (OH) of dry core-shell structure6。
Embodiment 4:
Step 1: by the SnCl of 0.5g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 1；
Step 2: by the Zn (Ac) of 0.33g2·2H2The SDBS of O and 0.25g is added in 10mL water, stirs 10min, is formed Solution 2；
It is mixed Step 3: solution 1 is stirred at room temperature with solution 2, the sodium hydroxide solution of 5mL 2moL/L is added dropwise；
Step 4: precipitating is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C of dryings Obtain the ZnSn (OH) of solid construction6。
Step 5: taking the ZnSn (OH) of 85mg solid construction6As presoma, 0.33gZn (Ac)2·2H2O's and 0.5g SDBS (neopelex) is added in 10mL water, stirs 10min, forms solution 3；
Step 6: taking the SnCl of 0.5g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 4；
Step 7: mixing is stirred at room temperature in solution 3 and solution 4, take the sodium hydroxide of 5mL 2mol/L that mixing is added In liquid；
Step 8: white precipitate is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C The ZnSn (OH) of dry core-shell structure6。
Embodiment 5:
Step 1: by the SnCl of 0.35g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 1；
Step 2: by the Zn (Ac) of 0.22g2·2H2The SDBS of O and 0.35g is added in 10mL water, stirs 10min, is formed Solution 2；
It is mixed Step 3: solution 1 is stirred at room temperature with solution 2, the sodium hydroxide solution of 5mL 2moL/L is added dropwise；
Step 4: precipitating is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C of dryings Obtain the ZnSn (OH) of solid construction6, Fig. 1 is the ZnSn (OH) of solid construction6Transmission electron microscope image.
Step 5: taking the ZnSn (OH) of 50mg solid construction6As presoma, 0.22gZn (Ac)2·2H2O's and 0.2g SDBS (neopelex) is added in 10mL water, stirs 10min, forms solution 3；
Step 6: taking the SnCl of 0.35g4·5H2O, which is added in 5mL ethyl alcohol, stirs 10min, forms solution 4；
Step 7: mixing is stirred at room temperature in solution 3 and solution 4, take the sodium hydroxide of 5mL 2mol/L that mixing is added In liquid；
Step 8: white precipitate is collected by centrifugation in solution obtained in the previous step, respectively washed three times with water and ethyl alcohol, 60 DEG C The ZnSn (OH) of dry core-shell structure6, Fig. 2 is the ZnSn (OH) of core-shell structure6Transmission electron microscope image.
It takes root testing example
1. wheat seed germinating is tested: according to experiment, it is 1mg/L, 5mg/L, 10mg/L that mass fraction, which is respectively configured, The core-shell structure ZnSn (OH) of 50mg/L, 100mg/L6, solid construction ZnSn (OH)6Solution.According to the nanometer system of each configuration The difference of agent concentration, the nanometer ZnS n (OH) of each concentration gradient6Solution corresponds to 20 seeds.It is put into small beaker and uses 1mg/ respectively The core-shell structure ZnSn (OH) of L, 5mg/L, 10mg/L, 50mg/L, 100mg/L6, solid construction ZnSn (OH)6Solution, impregnate 8-12h makes the abundant water swelling of seed.Culture dish is cleaned up, it is dry, 2 layers of blotting paper are placed in bottom, then, leaching The seed for steeping each concentration is transferred in culture dish, and seed is placed neatly as far as possible, in order to the observation in later period, is then being planted Sub- top puts one again and arrives layers of absorbent paper, 20, seed is put into each culture dish, according to each nanometer ZnS n (OH)6Solution Various concentration, each soak are arranged and nanometer ZnS n (OH)6Solution concentration gradient is consistent the culture dish of quantity.In each training Support the nanometer ZnS n (OH) that respective concentration is used in ware6(blotting paper in the test entirely germinateed in culture dish is all for solution wetted Guarantee to moisten, to guarantee that seed possesses sufficient moisture content in whole experiment process).With the extension of time, starting respectively 4h after seed soaking, 6h, 8.5h, 11h, 18.5h, 22h record the germination number of wheat seed in detail, in order to the later period Interpretation of result.
Germination percentage (%)=(seed number of all germinations in test/for test seed number) × 100%
Promotion rate (%)=(germination percentage of drug seed soaking-control group seed soaking germination percentage)/control group seed soaking germination percentage × 100%
Core-shell structure ZnSn (OH)6Influence data to wheat seed germinating are as shown in table 1 below, as can be seen from the table, With the extension of time, each preparation increases the germination percentage of wheat seed, when the same time being measured germination percentage, just like Lower rule, generally conforming to low concentration high concentration, the germination percentage of intermediate concentration is not high, at 5mg/L, 10mg/L concentration conditions Germination percentage is relatively high, illustrates the ZnSn (OH) of core-shell structure6Nanometer formulation low concentration or high concentration play seed Inhibiting effect.
1 core-shell structure ZnSn (OH) of table6Influence datagram to wheat seed germinating
Solid construction ZnSn (OH)6Influence data to wheat seed germinating are as shown in table 2 below, as can be seen from the table, Under the conditions of 1mg/L, 5mg/L, 10mg/L, solid construction nanometer ZnS n (OH)6Material is apparently higher than the germination percentage of Wheat Species Control group, with the extension of time, each concentration is not quite similar to the germination percentage compared with the control group situation of change of seed, In highest germination percentage be 10mg/L, in 22 hours germination percentages under the conditions of 50mg/L, reach 83.8%.
2 solid construction ZnSn (OH) of table6Influence datagram to wheat seed germinating
2. seed soaking growth-promoting root experiment: it is 1mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L that mass fraction, which is respectively configured, Core-shell structure ZnSn (OH)6, solid construction ZnSn (OH)6Solution and clear water control group.According to the nanometer ZnS n of each configuration (OH)6The difference of concentration, the nanometer ZnS n (OH) of each concentration gradient6Solution corresponds to 20 seeds.Small beaker is put into use respectively The core-shell structure ZnSn (OH) of 1mg/L, 5mg/L, 10mg/L, 50mg/L, 100mg/L6, solid construction ZnSn (OH)6Solution, 8-12h is impregnated, the abundant water swelling of seed is made.Culture dish is cleaned up, it is dry, 2 layers of blotting paper are placed in bottom, then, The seed for impregnating each concentration is transferred in culture dish, as far as possible neatly, in order to the observation in later period, then seed is placed It puts one again above seed and arrives layers of absorbent paper, 20, seed are put into each culture dish, according to each nanometer ZnS n (OH)6It is molten The various concentration of liquid, each soak are arranged and nanometer ZnS n (OH)6Solution concentration gradient is consistent the culture dish of quantity.Every The nanometer ZnS n (OH) of respective concentration is used in a culture dish6Solution wetted (the water suction in the test entirely germinateed in culture dish Paper will guarantee to moisten, to guarantee that seed possesses sufficient moisture content in whole experiment process).Seed in culture dish has about When the root long of 2mm comes out, set about preparing agar medium.The preparation of agar medium: according to the capacity of each small beaker The product of the quantity of (general 30mL) and culture medium configures.It comprises the concrete steps that in the solution according to each gradient concentration of every 100mL The agar solution of the agar powder tanning various concentration of 0.5g is added.It adds after agar powder beaker to be put in heating mantle and heats, Stirring, until agar solution stirs into a kind of solution of clear.Then agar solution is poured into the small of each number while hot In beaker, it is cooled to gelled state, is agar medium used.After the germination in culture dish, choose 5 long Gesture is consistent, and the good seed kind of upgrowth situation enters in the agar medium in small beaker.Specific method is: on present culture medium 5 skies are vertically pricked out with sharp mouth tweezers, are then vertically put into the root of the sending of seedling in hole with tweezers, and to guarantee to plant The root system of seedling can will be more conducive to the growth of seedling straight down in this way, while cannot say that the top half of seed immerses fine jade In rouge culture medium.After seed kind enters agar medium, (root of general seedling grows to the bottom of beaker after root development is qualified Portion), seedling is extracted, takes photo simultaneously in detail with the length of its root of vernier caliper measurement, the length of stem with distillation water infiltration Thin makes record.
2.1 core-shell structure ZnSn (OH)6The influence (seed soaking mode) taken root to wheat seed
The ZnSn (OH) of core-shell structure6The influence data taken root to wheat seed are as shown in table 3 below, can from table Out, with the increase of concentration, main root is long, lateral root is long and the trend of reduction after first increasing occurs in stem height, wherein in 10mg/L There is maximum value, the long promotion rate of main root reaches 4.76%, and lateral root promotion rate reaches 14.43%, and stem height promotion rate reaches 5.17%, Nanometer ZnS n (OH)6Material also has promotion in other concentration, only occurs negative increase under a concentration.
3 core-shell structure ZnSn (OH) of table6The influence data taken root to wheat seed
2.2 core-shell structure ZnSn (OH)6The influence taken root to wheat seed (base applies mode)
Core-shell structure ZnSn (OH)6The influence data taken root to wheat seed are as shown in table 4 below, as can be seen from the table, It is applied in base and (nanometer ZnS n (OH) is added in culture medium6) under the conditions of, said preparation has preferable promote in the promotion long to main root Into effect, the facilitation of opposite side root long is not obvious, or even negative growth occurs, and the data high to stem can be seen that promotion is made With weaker, only there is 1.61% promotion rate in 10mg/L.The facilitation of which is good apparently without seed soaking mode.
4 core-shell structure ZnSn (OH) of table6The influence data taken root to wheat seed
2.3 solid construction ZnSn (OH)6The influence (seed soaking mode) taken root to wheat seed
Solid construction ZnSn (OH)6The influence data taken root to wheat seed are as shown in table 5 below, as can be seen from the table, Nanometer ZnS n (OH)6For material when under the conditions of 10mg/L, 50mg/L, in main root length, lateral root is long and stem Gao Shangjun plays rush Into effect, but occur the inhibiting effect of various aspects in low concentration, but also will appear inhibition in the higher situation of concentration.
5 solid construction ZnSn (OH) of table6The influence data taken root to wheat seed
2.4 solid construction ZnSn (OH)6The influence taken root to wheat seed (base applies mode)
Solid construction ZnSn (OH)6The influence data taken root to wheat seed are as shown in table 6 below, as can be seen from the table, It is applied in base and (nanometer ZnS n (OH) is added in culture medium6) under the conditions of, nanometer ZnS n (OH)6Material is in 10mg/L, 50mg/L condition When lower, in main root length, lateral root is long and stem Gao Shangjun plays facilitation, and under other concentration conditions, the three of measurement All there is inhibiting effect in a aspect, illustrates nanometer ZnS n (OH)6Material must use suitable concentration in application, otherwise Positive interaction is not had to the growth of seed.
6 solid construction ZnSn (OH) of table6The influence data taken root to wheat seed
Control group and core-shell structure ZnSn (OH) are compared by seed soaking mode in conjunction with the above experimental data and attached drawing 36Material Expect the influence comparison diagram to wheat seed germinating, different nanometer ZnS n (OH)6Material is sprouted to the seed of plant and seedling It takes root and is studied in detail, by experimental data it can be found that solid construction nanometer ZnS n (oH)6Material is to Wheat Species Germination percentage is apparently higher than control group, solid construction ZnSn (OH)6When under the conditions of 10mg/L, 50mg/L, main root is long, lateral root is long Facilitation is played with stem Gao Shangjun；Core-shell structure ZnSn (OH)6Germination percentage is opposite at 5mg/L, 10mg/L concentration conditions It is higher, 1mg/L, 5mg/L, under the conditions of 10mg/L, the ZnSn (OH) of core-shell structure6Material occurs maximum value in 10mg/L, main Root long promotion rate reaches 4.76%, and lateral root promotion rate reaches 14.43%, and stem height promotion rate reaches 5.17%, it is seen then that core-shell structure ZnSn (OH)6Material has the effect of more significant growth-promoting root and seed is promoted to sprout.
Before forming core-shell structure, the ZnSn (OH) of solid construction6As presoma, pass through Zn (Ac)2.H2O and SDBS (neopelex) and SnCl4.5H2Zn in the addition of O, SDBS (neopelex) and solid construction2 +And Sn4+Network and, while again with the Zn in solution2+And Sn4+Network and, be wrapped in the ZnSn (OH) of solid construction6Outside passes through hydrogen The dropwise addition of sodium hydroxide solution, OH+With Zn2+、Sn2+Reaction, so that SDBS (neopelex) is cemented out, due to SDBS (neopelex) molecular proportion OH+Ion is big, forms a large amount of cavity in the inside of sphere after being replaced out, from And form the nanometer ZnS n (OH) of core-shell structure6Material.Due to the nanometer ZnS n (OH) of core-shell structure6Material internal exists empty Chamber discharges Zn2+And Sn4+Fast speed, so the nanometer ZnS n (OH) of core-shell structure6Nanometer ZnS n of the material than solid construction (OH)6The growth-promoting root of material and the effect for promoting seed to sprout are good.

Claims (10)

1. a kind of preparation method for the nano material for promoting seed to sprout and take root, which comprises the following steps:
Step 1, by SnCl4·5H2O is added in dehydrated alcohol and stirs, and forms solution 1；
Step 2, by Zn (Ac)2·2H2O and SDBS (neopelex) are added in distilled water, and stirring forms solution 2；
Step 3 according to the volume ratio of solution 1 and solution 2 is 1:2, and solution 1 is stirred at room temperature with solution 2 and is mixed, dropwise addition hydrogen Sodium hydroxide solution；
Step 4, by solution obtained in the previous step, precipitating is collected by centrifugation, is respectively washed three times with water and ethyl alcohol, 60 DEG C are solidly dry The ZnSn (OH) of structure6；
Step 5, the ZnSn (OH) by solid construction6As presoma, Zn (Ac)2·2H2O and SDBS are added in distilled water, stirring Form solution 3；
Step 6, by SnCl4·5H2O is added to stirring in dehydrated alcohol and forms solution 4；
Mixing is stirred at room temperature in solution 3 and solution 4 by step 7, and sodium hydroxide solution is added dropwise into mixed liquor；
Step 8, by solution obtained in the previous step, white precipitate is collected by centrifugation, is respectively washed three times with water and ethyl alcohol, 60 DEG C are dry The nanometer ZnS n (OH) of core-shell structure6Material.
2. the preparation method of nano material according to claim 1, it is characterised in that: SnCl in the step 14·5H2O Molar concentration be 0.0005~0.0014mol/L, mixing time 10min.
3. the preparation method of nano material according to claim 1, it is characterised in that: Zn described in step 2 (Ac)2· 2H2The molar concentration of O is 0.0005~0.0015mol/L, and the molar concentration of SDBS is 0.0005~0.0014mol/L, stirring Time is 10min.
4. the preparation method of nano material according to claim 1, it is characterised in that: sodium hydroxide is added dropwise described in step 3 Concentration be 2mol/L, the volume of the sodium hydroxide solution of dropwise addition and solution 1, the formation of solution 2 the volume ratio of mixed solution be 1:3。
5. the preparation method of nano material according to claim 1, it is characterised in that: ZnSn described in step 5 (OH)6's Molar concentration is 0.0001~0.0003mol/L, Zn (Ac)2·2H2The molar concentration of O is 0.0005~0.0015mol/L, The molar concentration of SDBS is 0.0005~0.0014mol/L, mixing time 10min.
6. the preparation method of nano material according to claim 1, it is characterised in that: SnCl described in step 64·5H2O The molar concentration of solution is 0.0005~0.0014mol/L, mixing time 10min.
7. the preparation method of nano material according to claim 1, it is characterised in that: sodium hydroxide is added dropwise described in step 7 Molar concentration be 2mol/L, the volume for the mixed solution that the volume of the sodium hydroxide solution of dropwise addition and solution 3, solution 4 are formed Than for 1:3.
8. nanometer ZnS n (OH) made from the preparation method of nano material described in any one of claim 1~76Material, It is characterized in that nanometer ZnS n (OH)6Material is a kind of core-shell structure.
9. nuclear shell structure nano ZnSn (OH) according to claim 86, which is characterized in that the nanometer ZnS n of core-shell structure (OH)6The presoma of material is the ZnSn (OH) of solid construction6Particle.
10. nano material described in claim 1 is in the application for promoting seed germination and taking root.
CN201910047604.4A 2019-01-17 2019-01-17 A kind of promotion seed sprouting and the nano material taken root and preparation method thereof Pending CN109607601A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110337857A (en) * 2019-08-12 2019-10-18 中国科学院东北地理与农业生态研究所 A method of improving Course of Corn Seed Germination, growth

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110337857A (en) * 2019-08-12 2019-10-18 中国科学院东北地理与农业生态研究所 A method of improving Course of Corn Seed Germination, growth

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Application publication date: 20190412