CN110337857A - A method of improving Course of Corn Seed Germination, growth - Google Patents
A method of improving Course of Corn Seed Germination, growth Download PDFInfo
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- CN110337857A CN110337857A CN201910740770.2A CN201910740770A CN110337857A CN 110337857 A CN110337857 A CN 110337857A CN 201910740770 A CN201910740770 A CN 201910740770A CN 110337857 A CN110337857 A CN 110337857A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
A method of Course of Corn Seed Germination, growth being improved, the present invention relates to crop planting fields.The invention solves the technical problems that existing corn seed germination rate is low, and the germination period is grown.Method: one, the surface of the seed sterilizes, two, seed soaking agent preparation, three, oscillation seed soaking.The present invention can be shortened the average time of germination, improve the germination percentage, germinating energy, germination index, vitality index of seed；Increase the growth and elongation of root system and plumule；Significantly increase hexokinase and urea glycosides diphosphate glucose pyrophosphorylation enzymatic activity, reduce aldolase activity, and maintain phosphofructokinase activity in opposite normal level, the biosynthetic process of glycolysis metabolism and cell wall is promoted, provides metabolite and energy for seed germination and growth.The present invention is for promoting Course of Corn Seed Germination, growth.
Description
Technical field
The present invention relates to crop planting fields.
Background technique
Corn (Zea mays L.) belongs to grass family (Gramineae) Zea, is important grain, economy and feeding dual-purpose crop,
Its yield is high, adaptable, has a very wide distribution, it has also become yield potential and the maximum cereal product of volume of trade.China is the world
The upper second largest maize production state, corn account for critical role in China's agricultural production and national economy.Corn yield direct relation
To the Food Security in China, as population is increasing, cultivated area is reduced year by year, soil fertility decline, scarcity of resources
And degeneration, the problems such as weather extramalization and increasing environmental pollution, realize highly efficient and productive, high-quality, low consumption modernization and sustainable
Agricultural is more and more important.China's corn increases total output mainly by per unit area yield is improved, and conventional Technique for Corn Cultivation has been unable to meet
The demand of production.And seed is the basis of corn growth breeding, full stand and healthy seedling is the basis of high crop yield, therefore improves kind
Sub- germination percentage increases closely related with corn yield.
Summary of the invention
The invention solves the technical problems that existing corn seed germination rate is low, and the germination period is grown, and the one kind provided mentions
High Course of Corn Seed Germination, growth method.
A method of Course of Corn Seed Germination, growth are improved, is sequentially included the following steps:
One, the surface of the seed sterilizes: the corn seed of full seed, no disease and pests harm is chosen, is sterilized using liquor natrii hypochloritis,
Then ultrapure water is used, natural air drying on filter paper is placed on；
Two, seed soaking agent is prepared: nano granular of zinc oxide is soluble in water, carry out ultrasonic mixing, be made concentration be 20~
100mg·L-1Nano zinc oxide suspension, i.e. seed soaking agent；
Three, it vibrates seed soaking: the corn seed of step 1 processing being submerged in seed soaking agent obtained in step 2, then
The oscillation treatment in constant temperature oscillator, is then sowed, and completes a kind of method for improving Course of Corn Seed Germination, growth.
Further, the mass fraction of liquor natrii hypochloritis is 1% in step 1.
Further, disinfecting time is 30min in step 1, is then used ultrapure water 5~6 times, natural air drying is for 24 hours.
Further, the average diameter of nano granular of zinc oxide is 20nm, specific surface area 21.5m in step 22g-1。
Further, when ultrasound mixes in step 2, control frequency is 40kHz, and mixing temperature is 40 DEG C, and mixing time is
1h。
Further, the concentration of nano zinc oxide suspension is 50mgL in step 2-1。
Further, soaking temperature is 28 DEG C in step 3.
Further, in step 3 when oscillation treatment, control revolving speed is 200r/min, duration of oscillation 12h.
Further, every 100mL seed soaking agent impregnates 100~120 corn seeds in step 3.
After nano zine oxide ultrasound suspending of the present invention, and oscillator is soaked seed, and is the sedimentation in order to avoid nano zine oxide,
It can allow nano zine oxide that can come into full contact with effect with corn seed；If dispersion is not added in static nano zinc oxide suspension
Agent can precipitate.If directly selecting the suspension of bonus point powder, influence of that dispersing agent to seed germination and growth
It needs to consider.
The beneficial effects of the present invention are:
The present invention can be shortened the average time of germination, improve germination percentage, germinating energy, germination index, the vigor of seed
Index；Increase the growth and elongation of root system and plumule；Significantly increase hexokinase and urea glycosides diphosphate glucose pyrophosphoric acid
Change enzymatic activity, reduces aldolase activity, and maintain phosphofructokinase activity in opposite normal level, promote glycolysis generation
It thanks to the biosynthetic process with cell wall, provides metabolite and energy for seed germination and growth.
Seed is after the present invention vibrates seed soaking, by carrying out glycometabolism key enzyme activity to the seed root system sprouted 7 days
Property measurement, concentration 50mg L of the present invention-1Seed soaking agent concussion seed soaking increases glycolysis key enzyme hexokinase and phosphoric acid Portugal
Grape sugar isomerase activity, facilitates the generation of pbosphohexose and Sugar signal, provides metabolin for cellular respiration and plant growth
Matter and energy；Aldolase activity is reduced, gluconeogenesis is inhibited, sprouts energy supply for seed；And increase Niao Gan diphosphonic acid Portugal
Grape sugar pyrophosphorylation enzymatic activity, is conducive to the synthesis of precursor substance cellulose, callose of cell-wall components etc..This illustrates this hair
The bright biosynthesis for promoting glycolysis metabolism and cell wall, may advantageously facilitate seed sprout, growth, germination percentage reach 97% with
On.
The present invention is for promoting Course of Corn Seed Germination, growth.
Detailed description of the invention
Fig. 1 is that percentage of seedgermination compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 2 is that potentiality of seed compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 3 is that seed germination index compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 4 is that Seed Integrated vigor Index compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 5 is that seed mean germination speed compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 6 is that 7 days root system hexokinases of seed sprouting compare figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 7 is that seed sprouts 7 days root system glucose phosphate isomerases after embodiment one, embodiment two and embodiment three are handled
Compare figure；
Fig. 8 is that 7 days root system aldolase activities of seed sprouting compare after embodiment one, embodiment two and embodiment three are handled
Figure；
Fig. 9 is that 7 days root system phosphofructokinases of seed sprouting compare after embodiment one, embodiment two and embodiment three are handled
Figure；
Figure 10 is that seed sprouts 7 days root system urea glycosides diphosphonic acid grapes after embodiment one, embodiment two and embodiment three are handled
Sugared pyrophosphorylase expression activitiy figure.
Specific embodiment
Technical solution of the present invention is not limited to the specific embodiment of act set forth below, further include each specific embodiment it
Between any combination.
Specific embodiment 1: present embodiment it is a kind of improve Course of Corn Seed Germination, growth method, according to the following steps into
Row:
One, the surface of the seed sterilizes: the corn seed of full seed, no disease and pests harm is chosen, is sterilized using liquor natrii hypochloritis,
Then ultrapure water is used, natural air drying on filter paper is placed on；
Two, seed soaking agent is prepared: nano granular of zinc oxide is soluble in water, carry out ultrasonic mixing, be made concentration be 20~
100mg·L-1Nano zinc oxide suspension, i.e. seed soaking agent；
Three, it vibrates seed soaking: the corn seed of step 1 processing being submerged in seed soaking agent obtained in step 2, then
The oscillation treatment in constant temperature oscillator, is then sowed, and completes a kind of method for improving Course of Corn Seed Germination, growth.
Specific embodiment 2: the present embodiment is different from the first embodiment in that: sodium hypochlorite is molten in step 1
The mass fraction of liquid is 1%.It is other same as the specific embodiment one.
Specific embodiment 3: the present embodiment is different from the first and the second embodiment in that: when being sterilized in step 1
Between be 30min, then use ultrapure water 5~6 times, natural air drying is for 24 hours.It is other the same as one or two specific embodiments.
Specific embodiment 4: unlike one of present embodiment and specific embodiment one to three: being received in step 2
The average diameter of rice Zinc oxide particles is 20nm, specific surface area 21.5m2g-1.One of other and specific embodiment one to three
It is identical.
Specific embodiment 5: unlike one of present embodiment and specific embodiment one to four: surpassing in step 2
When sound mixes, control frequency is 40kHz, and mixing temperature is 40 DEG C, mixing time 1h.Other and specific embodiment one to four
One of it is identical.
Specific embodiment 6: unlike one of present embodiment and specific embodiment one to five: being received in step 2
The concentration of rice zinc oxide suspension is 50mgL-1.It is other identical as one of specific embodiment one to five.
Specific embodiment 7: unlike one of present embodiment and specific embodiment one to six: being soaked in step 3
Kind temperature is 28 DEG C.It is other identical as one of specific embodiment one to six.
Specific embodiment 8: unlike one of present embodiment and specific embodiment one to seven: shaking in step 3
When swinging processing, control revolving speed is 200r/min, duration of oscillation 12h.It is other identical as one of specific embodiment one to seven.
Specific embodiment 9: unlike one of present embodiment and specific embodiment one to eight: every in step 3
100mL seed soaking agent impregnates 100~120 corn seeds.It is other identical as one of specific embodiment one to eight.
Beneficial effects of the present invention are verified using following embodiment:
Embodiment one:
A kind of method for improving Course of Corn Seed Germination, growth of the present embodiment, sequentially includes the following steps:
One, the surface of the seed sterilizes: choose the corn seed of full seed, no disease and pests harm, use mass fraction be 1% it is secondary
Then sodium chlorate solution's disinfection, disinfecting time 30min are used ultrapure water 5 times, be placed on filter paper natural air drying for 24 hours；
Two, seed soaking agent is prepared: nano granular of zinc oxide is soluble in water, ultrasonic mixing is carried out, and control frequency is 40kHz,
Mixing temperature is 40 DEG C, mixing time 1h, and it is 20mgL that concentration, which is made,-1Nano zinc oxide suspension, i.e. seed soaking agent；Wherein
The average diameter of nano granular of zinc oxide is 20nm, specific surface area 21.5m2g-1；
Three, it vibrates seed soaking: the corn seed of step 1 processing being submerged in seed soaking agent obtained in step 2, often
100mL seed soaking agent impregnates 1000 corn seeds, and control soaking temperature is 28 DEG C, then the oscillation treatment in constant temperature oscillator,
Control revolving speed is 200r/min, then duration of oscillation 12h sows, and completes described a kind of to improve Course of Corn Seed Germination, growth
Method.
Embodiment two:
A kind of method for improving Course of Corn Seed Germination, growth of the present embodiment, sequentially includes the following steps:
One, the surface of the seed sterilizes: choose the corn seed of full seed, no disease and pests harm, use mass fraction be 1% it is secondary
Then sodium chlorate solution's disinfection, disinfecting time 30min are used ultrapure water 5 times, be placed on filter paper natural air drying for 24 hours；
Two, seed soaking agent is prepared: nano granular of zinc oxide is soluble in water, ultrasonic mixing is carried out, and control frequency is 40kHz,
Mixing temperature is 40 DEG C, mixing time 1h, and it is 50mgL that concentration, which is made,-1Nano zinc oxide suspension, i.e. seed soaking agent；Wherein
The average diameter of nano granular of zinc oxide is 20nm, specific surface area 21.5m2g-1；
Three, it vibrates seed soaking: the corn seed of step 1 processing being submerged in seed soaking agent obtained in step 2, often
100mL seed soaking agent impregnates 1000 corn seeds, and control soaking temperature is 28 DEG C, then the oscillation treatment in constant temperature oscillator,
Control revolving speed is 200r/min, then duration of oscillation 12h sows, and completes described a kind of to improve Course of Corn Seed Germination, growth
Method.
Embodiment three:
A kind of method for improving Course of Corn Seed Germination, growth of the present embodiment, sequentially includes the following steps:
One, the surface of the seed sterilizes: choose the corn seed of full seed, no disease and pests harm, use mass fraction be 1% it is secondary
Then sodium chlorate solution's disinfection, disinfecting time 30min are used ultrapure water 5 times, be placed on filter paper natural air drying for 24 hours；
Two, seed soaking agent is prepared: nano granular of zinc oxide is soluble in water, ultrasonic mixing is carried out, and control frequency is 40kHz,
Mixing temperature is 40 DEG C, mixing time 1h, and it is 100mgL that concentration, which is made,-1Nano zinc oxide suspension, i.e. seed soaking agent；Wherein
The average diameter of nano granular of zinc oxide is 20nm, specific surface area 21.5m2g-1；
Three, it vibrates seed soaking: the corn seed of step 1 processing being submerged in seed soaking agent obtained in step 2, often
100mL seed soaking agent impregnates 1000 corn seeds, and control soaking temperature is 28 DEG C, then the oscillation treatment in constant temperature oscillator,
Control revolving speed is 200r/min, then duration of oscillation 12h sows, and completes described a kind of to improve Course of Corn Seed Germination, growth
Method.
By treated the corn seed sowing of embodiment one, embodiment two and embodiment three, after sprouting, to sprouting 7 days
The comparison of radicle and plumule upgrowth situation, as shown in table 1, wherein different lowercases indicate aobvious in the horizontal upper difference of α=0.05
It writes,
Table 1
Note: the test seed Germination Temperature is 22 DEG C, is carried out in artificial intelligence incubator；Different lowercase alphabets in table
Show in 0.05 horizontal upper significant difference；X ± SD, n=3.
The double-deck middling speed that 30 seeds that pure water seed soaking and embodiment one~tri- are handled respectively are laid in culture dish is qualitative
On filter paper, the 7d that germinates is put into 22 DEG C of dark culturing casees, and regular replenishment moisture ensures that filter paper is wet, and each processing is repeated 3 times.With
Radicle is at least up to seed length 1/2 and is used as germination standard, per record germination situation for 24 hours.The 7th day embryo is sprouted to seed
The growth datas such as root, radicel length, fresh weight and indefinite radical carry out quantitative analysis.Calculate each germination index.And it is sprouted in seed
Take seed root system within 7th day, the measurement for relative physiologic index
Fig. 1 is that percentage of seedgermination compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 2 is that potentiality of seed compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 3 is that seed germination index compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 4 is that Seed Integrated vigor Index compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 5 is that seed mean germination speed compares figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 6 is that 7 days root system hexokinases of seed sprouting compare figure after embodiment one, embodiment two and embodiment three are handled；
Fig. 7 is that seed sprouts 7 days root system glucose phosphate isomerases after embodiment one, embodiment two and embodiment three are handled
Compare figure；
Fig. 8 is that 7 days root system aldolase activities of seed sprouting compare after embodiment one, embodiment two and embodiment three are handled
Figure；
Fig. 9 is that 7 days root system phosphofructokinases of seed sprouting compare after embodiment one, embodiment two and embodiment three are handled
Figure；
Figure 10 is that seed sprouts 7 days root system urea glycosides diphosphonic acid grapes after embodiment one, embodiment two and embodiment three are handled
Sugared pyrophosphorylase expression activitiy figure.
Experiments have shown that through concentration 50mg L-1Seed soaking agent vibrates the corn seed germination rate of seed soaking, germinating energy, germination
Index, Seed Integrated vigor Index are higher by 11.25%, 34.47%, 31.5% and 80.48% respectively than blank control (pure water seed soaking)；It is flat
Equal germinating time also shortens 14.38% than control group；Root system fresh weight, bud fresh weight, radicle are long, plumule is long and indefinite radical difference
18.65%, 27.36%, 37.25%, 16.35% and 16.27% is improved than blank control group.
Seed is after the present invention vibrates seed soaking, by carrying out glycometabolism key enzyme activity to the seed root system sprouted 7 days
Property measurement, concentration 50mg L of the present invention-1Seed soaking agent concussion seed soaking increases glycolysis key enzyme hexokinase and phosphoric acid Portugal
Grape sugar isomerase activity, facilitates the generation of pbosphohexose and Sugar signal, provides metabolin for cellular respiration and plant growth
Matter and energy；Aldolase activity is reduced, gluconeogenesis is inhibited, sprouts energy supply for seed；And increase Niao Gan diphosphonic acid Portugal
Grape sugar pyrophosphorylation enzymatic activity, is conducive to the synthesis of precursor substance cellulose, callose of cell-wall components etc..This illustrates this hair
The bright biosynthesis for promoting glycolysis metabolism and cell wall may advantageously facilitate seed and sprout, grow.
Claims (9)
1. a kind of method for improving Course of Corn Seed Germination, growth, it is characterised in that this method sequentially includes the following steps:
One, the surface of the seed sterilizes: choosing the corn seed of full seed, no disease and pests harm, is sterilized using liquor natrii hypochloritis, then
With ultrapure water, it is placed on natural air drying on filter paper；
Two, seed soaking agent is prepared: nano granular of zinc oxide is soluble in water, ultrasonic mixing is carried out, it is 20~100mg that concentration, which is made,
L-1Nano zinc oxide suspension, i.e. seed soaking agent；
Three, it vibrates seed soaking: the corn seed of step 1 processing being submerged in seed soaking agent obtained in step 2, then in perseverance
Oscillation treatment in warm oscillator, is then sowed, and completes a kind of method for improving Course of Corn Seed Germination, growth.
2. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that secondary in step 1
The mass fraction of sodium chlorate solution is 1%.
3. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that disappear in step 1
The malicious time is 30min, is then used ultrapure water 5~6 times, natural air drying is for 24 hours.
4. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that received in step 2
The average diameter of rice Zinc oxide particles is 20nm, specific surface area 21.5m2g-1。
5. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that surpass in step 2
When sound mixes, control frequency is 40kHz, and mixing temperature is 40 DEG C, mixing time 1h.
6. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that received in step 2
The concentration of rice zinc oxide suspension is 50mgL-1。
7. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that soaked in step 3
Kind temperature is 28 DEG C.
8. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that shake in step 3
When swinging processing, control revolving speed is 200r/min, duration of oscillation 12h.
9. a kind of method for improving Course of Corn Seed Germination, growth according to claim 1, it is characterised in that every in step 3
100mL seed soaking agent impregnates 100~120 corn seeds.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111667883A (en) * | 2020-06-03 | 2020-09-15 | 四川大学 | Forensic medicine mixed DNA analysis method based on composite micro-haplotype pyrophosphate sequencing atlas analysis |
| CN111700072A (en) * | 2020-06-22 | 2020-09-25 | 贵州大学 | Initiator for brewing sorghum seeds, production line and processing method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN111700072A (en) * | 2020-06-22 | 2020-09-25 | 贵州大学 | Initiator for brewing sorghum seeds, production line and processing method |
| CN111700072B (en) * | 2020-06-22 | 2021-07-06 | 贵州大学 | Initiator for brewing sorghum seeds, production line and processing method |
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