CN109576202A - A kind of breeding method improving number of live bacteria of probiotics using Chinese medicine additive - Google Patents
A kind of breeding method improving number of live bacteria of probiotics using Chinese medicine additive Download PDFInfo
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Abstract
The invention discloses a kind of breeding methods that number of live bacteria of probiotics is improved using Chinese medicine additive, comprising: Step 1: preparing the grain culture medium raw material using Chinese medicine additive respectively in parts by weight: 0.5~1.5 part of Chinese medicine additive, 12~25 parts of corncob, 10~25 parts of rice bran, 3~8 parts of wheat bran, 5~10 parts of oat, 10~15 parts of buckwheat, 2~5 parts of sorghum rice, 2~5 parts of maize, 5~8 parts of Semen Coicis, 10~15 parts of soybean, 10~15 parts of mung bean, 10~15 parts of semen sojae atricolor, 10~15 parts of soya bean；Step 2: the raw material is successively put into agitated kettle, carry out dry stirring, all raw material is stirred for after being stirring evenly and then adding into 20~30 DEG C of water, moisture content in medium is controlled 88~93%, after mixing evenly, the 25~30min that sterilizes under the conditions of 115 DEG C, 0.1Mpa handles to obtain grain culture medium；Step 3: seed liquor is made after recovery expands in the probiotics strain wait cultivate；Increasing bacterium is carried out Step 4: the seed liquor is inoculated into the grain culture medium, adjusting increasing bacterium temperature is 37 DEG C, and the Zengjing Granule time is 18~25h.
Description
Technical field
The present invention relates to field of microbial fermentation, and in particular to a kind of to improve number of live bacteria of probiotics using Chinese medicine additive
Breeding method.
Background technique
Probiotics is that one kind is colonized in human body intestinal canal, in reproductive system, can generate definite health efficacy so as to improve host
Microecological balance, the general name of the microorganism of the work to play an important role to human health, is widely used in food, health care product and medicine
In product production.The study found that quantity of the probiotics in human body intestinal canal plays health care to probiotics and therapeutic efficiency plays decision
Property effect guarantee its survival with storage period in process in addition to probiotics is proliferated in vivo and keep bioactivity etc.
Outside factor, enough viable counts are the prerequisites that Probiotic supplement product plays its validity.
Vegetalitas cereal materials are culture medium, and as fermentation substrate, cereal can not only provide for the growth of probiotics must
The carbon source and nitrogen source wanted reduce fermentation costs；Cereal after everfermentation can significantly improve its nutritive value and function is special
Property, the fragrance ingredients such as lactic acid, ethyl acetate, acetaldehyde for generating after fermentation can improve the flavor and fragrance of cereal products.Cereal is made
It can provide growth necessary nutriment for it for probiotics fermention matrix, Effective multiplication probiotics, but these grain culture mediums
It is both needed to the complex process technique such as carry out complicated pretreatment, including remove kind of a skin, boiling, enzymatic hydrolysis before carrying out fermented and cultured.Paddy
Object swelling powder is made through high temperature, high pressure swelling, can reduce the loss of nutrition in maturing process to greatest extent.It is produced through extruding
Raw high temperature and pressure, high shear force can make the starch granules extruding in cereal and essence, open starch molecule connection, increase
The surface area of chyme particle, the digestibility for improving starch；Extruding makes protein peptide chain be cracked into peptide and amino acid simultaneously,
Greatly improve the absorptivity of protein.Zymotechnique can effectively be simplified using puffed cereal, shorten fermentation period, to drop
Low fermentation costs.
Meanwhile many food, that is, drugs, absolute line of demarcation is had no between them, the Chinese medicine of many integration of drinking and medicinal herbs can
To promote the number of viable of probiotics very well, using vegetalitas cereal materials as the fermentation substrate of culture medium, it is properly added Chinese medicine
Additive can preferably improve the property of probiotics.
Summary of the invention
The present invention has designed and developed a kind of breeding method that number of live bacteria of probiotics is improved using Chinese medicine additive, the present invention
Goal of the invention first is that by the culture medium in cultivating process add Chinese medicine additive and rationally control its additive amount,
Simultaneously during the preparation process according to material quality number to dry mixing time, be stirred for time, seed inoculation liquid and Zengjing Granule
Time is accurately controlled, and maximum viable count can be significantly improved.
Goal of the invention of the invention second is that granular size to Chinese medicine additive and at the granular size of corncob
After reason, it can be good at improving maximum viable count.
Technical solution provided by the invention are as follows:
A kind of breeding method being improved number of live bacteria of probiotics using Chinese medicine additive, is included the following steps:
Step 1: preparing the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
0.5~1.5 part of Chinese medicine additive, 12~25 parts of corncob, 10~25 parts of rice bran, 3~8 parts of wheat bran, oat 5~
10 parts, 10~15 parts of buckwheat, 2~5 parts of sorghum rice, 2~5 parts of maize, 5~8 parts of Semen Coicis, 10~15 parts of soybean, mung bean 10~15
Part, 10~15 parts of semen sojae atricolor, 10~15 parts of soya bean；
Step 2: the raw material is successively put into agitated kettle, dry stirring is carried out, all raw material adds after mixing evenly
Be stirred for after entering 20~30 DEG C of water, by moisture content in medium control 88~93%, after mixing evenly, 115 DEG C,
25~the 30min that sterilizes under the conditions of 0.1Mpa handles to obtain grain culture medium；
Step 3: seed liquor is made after recovery expands in the probiotics strain wait cultivate；
Increasing bacterium is carried out Step 4: the seed liquor is inoculated into the grain culture medium, adjusting increasing bacterium temperature is 37
DEG C, the Zengjing Granule time is 18~25h；
Wherein, the mass fraction of component and each component is as follows in the Chinese medicine additive:
2~4 parts of Radix Notoginseng, 1~1.5 part of Radix Salviae Miltiorrhizae, 5~8 parts of Chinese yam, 10~13 parts of Radix Astragali, 15~25 parts of pueraria lobata, radix paeoniae rubra 1~
3 parts, 1~3 part of Rhizoma Atractylodis Macrocephalae, 2~8 parts of cowberry, 15~25 parts of honeysuckle, 10~18 parts of fructus lycii.
Preferably, the seed liquor inoculative proportion is
In formula, WDFor the mass fraction of Chinese medicine additive, WCFor the mass fraction of corncob, WBFor the quality of bean product
Number, WRFor the mass fraction of sorghum rice and maize and.
Preferably, in the step 4, the Zengjing Granule time is
In formula, WDFor the mass fraction of Chinese medicine additive, WOFor remove Chinese medicine additive other components mass fraction and,
WBFor the mass fraction of bean product, WRAnd, t is Zengjing Granule time, t for the mass fraction of sorghum rice and maizemaxTo increase bacterium
Cultivate maximum time, tminFor Zengjing Granule minimum time, teFor Zengjing Granule executeaaafunction,For Zengjing Granule time adjustment
Coefficient, η are Zengjing Granule executeaaafunction correction coefficient, and e is the truth of a matter of natural logrithm.
Preferably, in the step 3, the seed liquor preparation process are as follows:
The probiotics strain is added in MRS culture medium with 1% volume ratio, 37 DEG C static gas wave refrigerator 12 hours
Carry out the recovery of strain；Recovery bacterium solution is inoculated into the MRS culture medium by 10% volume ratio, 37 DEG C of static gas wave refrigerators
Carry out within 12 hours the preparation of probiotics bacterial seed liquor；
The MRS culture medium sterilizes under 115 DEG C, 0.1Mpa 30min after configuring, and natural cooling uses after sterilizing.
Preferably, the component of the MRS culture medium and mass fraction are as follows:
20 parts of peptone, 10 parts of beef extract, 5 parts of yeast extract, 4 parts of diammonium hydrogen citrate, 20 parts of glucose, Tween 80
3.2 parts, 5 parts of sodium acetate, 2 parts of dipotassium hydrogen phosphate, 0.25 part of magnesium sulfate, 0.05 part of manganese sulfate, 18 parts of agar, distilled water 1000
Part.
Preferably, in the step 2, the dry mixing time is 30~50 minutes, it is described be stirred for as 80~
100 minutes.
Preferably, in the step 2, the dry mixing time is
In formula, WCFor the mass fraction of corncob, WBFor the mass fraction of bean product, WRFor the matter of sorghum rice and maize
Measure number and T1For dry mixing time, T1_maxFor dry stirring maximum time, T1_minFor dry stirring minimum time, T1_eIt is stirred to be dry
Mix executeaaafunction, δ1For dry mixing time correction coefficient, γ1For dry stirring executeaaafunction correction coefficient.
Preferably, described to be stirred for the time and be
In formula, WCFor the mass fraction of corncob, WBFor the mass fraction of bean product, WRFor the matter of sorghum rice and maize
Measure number and T2To be stirred for time, T2_maxTo be stirred for maximum time, T2_minTo be stirred for minimum time, T2_eTo stir again
Mix executeaaafunction, δ2To be stirred for time adjustment coefficient, γ2To be stirred for executeaaafunction correction coefficient.
Preferably, δ1Value is 1.43~1.55, γ1Value is 0.23, δ2Value is 2.35~2.47, γ2Value is
1.13。
Preferably,Value is that 1.3~1.5, η value is 2.3.
The present invention compared with prior art possessed by the utility model has the advantages that
1, the present invention provides a kind of breeding method for improving number of live bacteria of probiotics using Chinese medicine additive, this hair is used
The culture medium of bright offer carries out probiotics cultivation, by adding Chinese medicine additive and rationally controlling its additive amount, simultaneously according to original
Material mass fraction to dry mixing time, be stirred for time, seed inoculation liquid and Zengjing Granule time and accurately controlled, can
Significantly improve maximum viable count；
It 2, can be fine after the present invention handles the granular size of Chinese medicine additive and the granular size of corncob
Raising maximum viable count.
Specific embodiment
The present invention is described in further detail below, to enable those skilled in the art's refer to the instruction text can
Implement accordingly.
The present invention provides it is a kind of using Chinese medicine additive improve number of live bacteria of probiotics grain culture medium, component and
The mass fraction of each component is as follows:
Chinese medicine additive, 12~25 parts of corncob, 10~25 parts of rice bran, 3~8 parts of wheat bran, 5~10 parts of oat, buckwheat 10
~15 parts, 2~5 parts of sorghum rice, 2~5 parts of maize, 5~8 parts of Semen Coicis, 10~15 parts of soybean, 10~15 parts of mung bean, semen sojae atricolor 10~
15 parts, 10~15 parts of soya bean, water content is 88%~93%；
Wherein, the mass fraction of Chinese medicine additive is
In formula, WDFor the mass fraction of Chinese medicine additive, WCFor the mass fraction of corncob, WBFor the quality of bean product
Number, WRAnd, e is the truth of a matter of natural logrithm for the mass fraction of sorghum rice and maize, and λ is experience adjustments coefficient；
The mass fraction of component and each component is as follows in Chinese medicine additive:
2~4 parts of Radix Notoginseng, 1~1.5 part of Radix Salviae Miltiorrhizae, 5~8 parts of Chinese yam, 10~13 parts of Radix Astragali, 15~25 parts of pueraria lobata, radix paeoniae rubra 1~
3 parts, 1~3 part of Rhizoma Atractylodis Macrocephalae, 2~8 parts of cowberry, 15~25 parts of honeysuckle, 10~18 parts of fructus lycii.
The present invention also provides use Chinese medicine additive improve number of live bacteria of probiotics grain culture medium preparation method,
It includes the following steps:
Step 1: prepare the grain culture medium raw material respectively in parts by weight:
Chinese medicine additive, 10~25 parts of rice bran, 3~8 parts of wheat bran, 5~10 parts of oat, 10~15 parts of buckwheat, sorghum rice 2
~5 parts, 2~5 parts of maize, 5~8 parts of Semen Coicis, 10~15 parts of soybean, 10~15 parts of mung bean, 10~15 parts of semen sojae atricolor, soya bean 10~
15 parts；
Wherein, the mass fraction of Chinese medicine additive is
In formula, WDFor the mass fraction of Chinese medicine additive, WCFor the mass fraction of corncob, WBFor the quality of bean product
Number, WRAnd, e is the truth of a matter of natural logrithm for the mass fraction of sorghum rice and maize, and λ is experience adjustments coefficient；
The mass fraction of component and each component is as follows in Chinese medicine additive:
2~4 parts of Radix Notoginseng, 1~1.5 part of Radix Salviae Miltiorrhizae, 5~8 parts of Chinese yam, 10~13 parts of Radix Astragali, 15~25 parts of pueraria lobata, radix paeoniae rubra 1~
3 parts, 1~3 part of Rhizoma Atractylodis Macrocephalae, 2~8 parts of cowberry, 15~25 parts of honeysuckle, 10~18 parts of fructus lycii.
Step 2: successively putting into the raw material in agitated kettle after expanding treatment, carries out dry stirring, all raw material
It is stirring evenly and then adding into 20~30 DEG C of water to be stirred again, moisture content in medium control is stirred for 88~93% after adding water
After uniformly, grain culture medium is obtained, which is sterilized 25~30min under the conditions of 115 DEG C, 0.1Mpa.
The present invention also provides the breeding methods for using Chinese medicine additive to improve number of live bacteria of probiotics comprising following step
It is rapid:
Step 1 prepares to use the grain culture medium raw material of Chinese medicine additive in parts by weight respectively:
Chinese medicine additive, 12~25 parts of corncob, 10~25 parts of rice bran, 3~8 parts of wheat bran, 5~10 parts of oat, buckwheat 10
~15 parts, 2~5 parts of sorghum rice, 2~5 parts of maize, 5~8 parts of Semen Coicis, 10~15 parts of soybean, 10~15 parts of mung bean, semen sojae atricolor 10~
15 parts, 10~15 parts of soya bean；
Wherein, the mass fraction of Chinese medicine additive is
In formula, WDFor the mass fraction of Chinese medicine additive, WCFor the mass fraction of corncob, WBFor the quality of bean product
Number, WRAnd, e is the truth of a matter of natural logrithm for the mass fraction of sorghum rice and maize, and λ is experience adjustments coefficient；
The mass fraction of component and each component is as follows in Chinese medicine additive:
2~4 parts of Radix Notoginseng, 1~1.5 part of Radix Salviae Miltiorrhizae, 5~8 parts of Chinese yam, 10~13 parts of Radix Astragali, 15~25 parts of pueraria lobata, radix paeoniae rubra 1~
3 parts, 1~3 part of Rhizoma Atractylodis Macrocephalae, 2~8 parts of cowberry, 15~25 parts of honeysuckle, 10~18 parts of fructus lycii.
Step 2 successively puts into the raw material in agitated kettle after expanding treatment, carries out dry stirring, and all raw material stirs
20~30 DEG C of water is added after mixing uniformly to be stirred again, moisture content in medium control is stirred for 88~93% after adding water
After even, grain culture medium is obtained, which is sterilized 25~30min under the conditions of 115 DEG C, 0.1Mpa；
The preparation of step 3, probiotics seed liquor: by the probiotics strain of preservation, being made seed liquor after recovery expands,
Probiotics strain is added in modified MRS culture medium with the ratio of 1% (v/v), 37 DEG C of static gas wave refrigerators, 12 hours progress strains
Recovery；Recovery bacterium solution is inoculated into modified MRS culture medium in the ratio of 10% (v/v), 37 DEG C of static gas wave refrigerators 12 hours into
The preparation of row probiotics bacterial seed liquor sterilizes under 115 DEG C, 0.1Mpa after modified lactic acid bacteria culture medium (modified MRS) configuration is good
30min, natural cooling after sterilizing, formula are as shown in table 1；
1 modified MRS culture medium of table
Step 4, probiotics Zengjing Granule: the probiotics seed liquor that step 3 obtains is inoculated into the increasing that step 2 obtains
Increasing bacterium is carried out in bacterium culture medium, Zengjing Granule temperature is 37 DEG C.
In another embodiment, probiotics strain is lactobacillus plantarum, lactobacillus bulgaricus, Lactobacillus casei, excrement
Enterococcus, Lactobacillus salivarius, Lactobacillus rhamnosus, lactobacillus fermenti
In another embodiment, Chinese medicine additive is processed, the Chinese medicine additive particle is made to meet following item
Part: the particle fraction that mesh granularity is greater than 120 mesh is 5~10%, mesh granularity be the particle fraction of 100~120 mesh be 30~
35%, mesh granularity is that the particle fraction of 80~100 mesh is 40~45%, and surplus is particle of the mesh granularity less than 80 mesh.
In another embodiment, corncob is processed, the corn cob granule is made to meet following condition:
The ratio that particle is 7mm~9mm is 1%~3%, and the ratio that particle is 3mm~7mm is 75%~85%, and surplus is that particle is small
In the particle of 3mm, also, the water content of the corn core raw material is 5%~8%.
In another embodiment, dry mixing time is 30~50 minutes, and being stirred for the time is 80~100 minutes, seed
The inoculative proportion that liquid adds to enriched medium is 3% (volume ratio, v/v), and the Zengjing Granule time is 18~25h.
In another embodiment, dry mixing time is
Being stirred for the time is
In formula, WCFor the mass fraction of corncob, WBFor the mass fraction of bean product, WRFor the matter of sorghum rice and maize
Measure number and T1For dry mixing time, T1_maxFor dry stirring maximum time, T1_minFor dry stirring minimum time, T1_eIt is stirred to be dry
Mix executeaaafunction, δ1For dry mixing time correction coefficient, γ1For dry stirring executeaaafunction correction coefficient, T2To be stirred for the time,
T2_maxTo be stirred for maximum time, T2_minTo be stirred for minimum time, T2_eTo be stirred for executeaaafunction, δ2To be stirred for the time
Correction coefficient, γ2To be stirred for executeaaafunction correction coefficient.
In another embodiment, seed liquor inoculative proportion is
In formula, WDFor the mass fraction of Chinese medicine additive, WCFor the mass fraction of corncob, WBFor the quality of bean product
Number, WRFor the mass fraction of sorghum rice and maize and.
In another embodiment, the Zengjing Granule time is
In formula, WDFor the mass fraction of Chinese medicine additive, WOFor remove Chinese medicine additive other components mass fraction and,
WBFor the mass fraction of bean product, WRAnd, t is Zengjing Granule time, t for the mass fraction of sorghum rice and maizemaxTo increase bacterium
Cultivate maximum time, tminFor Zengjing Granule minimum time, teFor Zengjing Granule executeaaafunction,For Zengjing Granule time adjustment
Coefficient, η are Zengjing Granule executeaaafunction correction coefficient, and e is the truth of a matter of natural logrithm.
In another embodiment, λ value is 0.38~0.45, δ1Value is 1.43~1.55, γ1Value is 0.23, δ2
Value is 2.35~2.47, γ2Value is 1.13,Value is that 1.3~1.5, η value is 2.3.
Embodiment 1
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
12 parts of corncob, 10 parts of rice bran, 3 parts of wheat bran, 5 parts of oat, 10 parts of buckwheat, 2 parts of sorghum rice, 2 parts of maize, Semen Coicis 5
Part, 10 parts of soybean, 10 parts of mung bean, 10 parts of semen sojae atricolor, 10 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.38；
The mass fraction of component and each component is as follows in Chinese medicine additive:
2 parts of Radix Notoginseng, 1 part of Radix Salviae Miltiorrhizae, 5 parts of Chinese yam, 10 parts of Radix Astragali, 15 parts of pueraria lobata, 1 part of radix paeoniae rubra, 1 part of Rhizoma Atractylodis Macrocephalae, 2 parts of cowberry, gold
15 parts of honeysuckle flower, 10 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 5%, and mesh granularity is that the particle fraction of 100~120 mesh is 30%, and mesh granularity is the particle fraction of 80~100 mesh
It is 40%, surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 1%, and the ratio that particle is 3mm~7mm is 75%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 5%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.43, γ1Value is 0.23, δ2Value is 2.35, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.3, η value is 2.3.
Embodiment 2
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Embodiment 3
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
18 parts of corncob, 15 parts of rice bran, 5 parts of wheat bran, 8 parts of oat, 13 parts of buckwheat, 3 parts of sorghum rice, 4 parts of maize, Semen Coicis 7
Part, 13 parts of soybean, 12 parts of mung bean, 13 parts of semen sojae atricolor, 12 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.4；
The mass fraction of component and each component is as follows in Chinese medicine additive:
3 parts of Radix Notoginseng, 1.3 parts of Radix Salviae Miltiorrhizae, 6 parts of Chinese yam, 11 parts of Radix Astragali, 18 parts of pueraria lobata, 2 parts of radix paeoniae rubra, 2 parts of Rhizoma Atractylodis Macrocephalae, 5 parts of cowberry,
21 parts of honeysuckle, 13 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 8%, and mesh granularity is that the particle fraction of 100~120 mesh is 33%, and mesh granularity is the particle fraction of 80~100 mesh
It is 43%, surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 2%, and the ratio that particle is 3mm~7mm is 80%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 7%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.5, γ1Value is 0.23, δ2Value is 2.4, γ2Value is 1.13, T1_minFor 30min,
T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor for 24 hours,Value is that 1.45, η value is 2.3.
Comparative example 1
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, Chinese medicine additive particle is made to meet 80~100 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 2
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, meets corn cob granule and is less than 9mm；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 3
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
1.5 parts of Chinese medicine additive, 25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, sorghum rice
5 parts, 5 parts of maize, 8 parts of Semen Coicis, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 4
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is 30 minutes；
Being stirred for the time is
In formula, δ2Value is 2.47, γ2Value is 1.13, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 5
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is 50 minutes；
Being stirred for the time is
In formula, δ2Value is 2.47, γ2Value is 1.13, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 6
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is 80 minutes；
In formula, δ1Value is 1.55, γ1Value is 0.23, T1_minFor 30min, T1_maxFor 50min, T1_eFor 45min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 7
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is 100 minutes；
In formula, δ1Value is 1.55, γ1Value is 0.23, T1_minFor 30min, T1_maxFor 50min, T1_eFor 45min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 8
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is 3% (volume ratio, v/v)；
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Comparative example 9
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is 18h.
Comparative example 10
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The mass fraction of Chinese medicine additive is
In formula, λ value is 0.45；
The mass fraction of component and each component is as follows in Chinese medicine additive:
4 parts of Radix Notoginseng, 1.5 parts of Radix Salviae Miltiorrhizae, 8 parts of Chinese yam, 13 parts of Radix Astragali, 15 parts of pueraria lobata, 3 parts of radix paeoniae rubra, 3 parts of Rhizoma Atractylodis Macrocephalae, 8 parts of cowberry,
25 parts of honeysuckle, 18 parts of fructus lycii；
Chinese medicine additive is processed, so that Chinese medicine additive particle is met following condition: mesh granularity is greater than 120 purposes
Particle fraction is 10%, and mesh granularity is that the particle fraction of 100~120 mesh is 35%, and mesh granularity is the particle ratio of 80~100 mesh
Example is 45%, and surplus is particle of the mesh granularity less than 80 mesh；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is 25h.
Comparative example 11
Prepare the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
25 parts of corncob, 25 parts of rice bran, 8 parts of wheat bran, 10 parts of oat, 15 parts of buckwheat, 5 parts of sorghum rice, 5 parts of maize, Semen Coicis
8 parts, 15 parts of soybean, 15 parts of mung bean, 15 parts of semen sojae atricolor, 15 parts of soya bean；
The corncob is processed, corn cob granule is made to meet following condition: the ratio of particle 7mm~9mm
Example is 3%, and the ratio that particle is 3mm~7mm is 85%, and surplus is the particle that particle is less than 3mm, also, the corncob is former
The water content of material is 8%；
Dry mixing time is
Being stirred for the time is
In formula, δ1Value is 1.55, γ1Value is 0.23, δ2Value is 2.47, γ2Value is 1.13, T1_minFor
30min, T1_maxFor 50min, T1_eFor 45min, T2_minFor 80min, T2_maxFor 100min, T2_eFor 95min；
Lactobacillus plantarum is selected to carry out preparation seed liquor；
The inoculative proportion that seed liquor adds to enriched medium is
The Zengjing Granule time is
In formula, tminFor 20h, tmaxFor 25h, teFor 22.5h,Value is that 1.5, η value is 2.3.
Test example
Viable count measurement is carried out to Examples 1 to 3 and comparative example 1~11, is measured and is fermented using Flow cytometry method
Probiotics bacterium number in liquid obtains the viable count of probiotics and dead bacterium number in cereal fermentation liquor.Flow cytometry method it is specific
Operation is as described below:
The fermentation liquid of taking-up is carried out dilution appropriate, makes viable count in bacteria liquid sample to be detected by step 1, preparation of samples
Quantitative range 5.5 × 105~6.5 × 105CFU/mL.Fermentation broth sample after dilution is filtered through 300 mesh gauzes, takes dilution
200 μ L of measuring samples afterwards is added in the EP pipe of 1.5mL, in 37 DEG C constant incubator warm bath 3 minutes；
Step 2, sample dyeing, be added in 200 μ L of measuring samples 2 μ L 100 × Sybr Green I and 2 μ L 100 ×
PI, 37 DEG C are protected from light dyeing 10 minutes.Sybr Green I and PI (Propidium Iodide) are two kinds of common DNA fluorescence
Dyestuff.Sybr Green I can penetrate the cell membrane of living cells and dead cell, and PI cannot penetrate the cell of living cells well
Film.Using Sybr Green I and PI stained bacteria, the work that bacterium can be divided into three crowds: Sybr Green I (+) PI (-) is thin
Bacterium, the dead bacterium of Sybr Green I (+) PI (+), and the damage bacterium between two groups；
Step 3, C6Accuri absolute counting, C6Accuri flow cytometry analysis sample, threshold value are set as FSC-H and are greater than
10000, flow velocity selects at a slow speed, and sampling quantity is set as 60 μ L, carries out absolute counting detection；
Step 4, using FL1-H as abscissa, FL2-A is ordinate, draw cell density figure.By analysis water-like density map,
Bacterium solution after bacterium solution and heating divides group according to the cell of these samples, and circle gating counts；
Experimental result is as shown in table 2, carries out viable bacteria experiment by the present invention treated culture medium, Examples 1 to 3
Maximum viable count is 5.5 × 108CFU/mL or more, it can reach 6.1 using the culture medium maximum viable count in embodiment 2 ×
108CFU/mL is obviously improved compared with the culture medium (comparative example 11) for being not added with Chinese medicine additive, while simple with Chinese medicine
The culture medium (comparative example 1) of single working process, the untreated culture medium of corncob (comparative example 2), the non-root of Chinese medicine additive number
According to the culture medium (comparative example 3) of material quality number precision processing, dry mixing time is refined not according to material quality number
The culture medium (comparative example 4,5) of processing is stirred for the time not according to the culture of material quality number precision processing after adding water
Base (comparative example 6,7), seed liquor inoculative proportion not according to material quality number precision processing culture medium (comparative example 8) and
The Zengjing Granule time is significantly improved not according to the culture medium (comparative example 9,10) of material quality number precision processing.
The maximum viable count of table 2
| Embodiment | Maximum viable count (CFU/mL) |
| Embodiment 1 | 5.5×108 |
| Embodiment 2 | 6.1×108 |
| Embodiment 3 | 5.8×108 |
| Comparative example 1 | 4.4×108 |
| Comparative example 2 | 4.3×108 |
| Comparative example 3 | 5.0×108 |
| Comparative example 4 | 2.4×108 |
| Comparative example 5 | 3.2×108 |
| Comparative example 6 | 3.5×108 |
| Comparative example 7 | 3.1×108 |
| Comparative example 8 | 2.8×108 |
| Comparative example 9 | 4.0×108 |
| Comparative example 10 | 4.1×108 |
| Comparative example 11 | 1.3×108 |
Although the embodiments of the present invention have been disclosed as above, but its institute not only in the description and the implementation
Column use, it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can hold
It changes places and realizes other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously
It is not limited to specific details.
Claims (10)
1. a kind of breeding method for improving number of live bacteria of probiotics using Chinese medicine additive, which comprises the steps of:
Step 1: preparing the grain culture medium raw material using Chinese medicine additive respectively in parts by weight:
0.5~1.5 part of Chinese medicine additive, 12~25 parts of corncob, 10~25 parts of rice bran, 3~8 parts of wheat bran, 5~10 parts of oat,
It is 10~15 parts of buckwheat, 2~5 parts of sorghum rice, 2~5 parts of maize, 5~8 parts of Semen Coicis, 10~15 parts of soybean, 10~15 parts of mung bean, black
10~15 parts of beans, 10~15 parts of soya bean；
Step 2: the raw material is successively put into agitated kettle, dry stirring is carried out, all raw material is stirring evenly and then adding into 20~
It is stirred for after 30 DEG C of water, moisture content in medium is controlled 88~93%, after mixing evenly, in 115 DEG C, 0.1Mpa item
25~the 30min that sterilizes under part handles to obtain grain culture medium；
Step 3: seed liquor is made after recovery expands in the probiotics strain wait cultivate；
Increasing bacterium is carried out Step 4: the seed liquor is inoculated into the grain culture medium, adjusting increasing bacterium temperature is 37 DEG C, increases bacterium
Incubation time is 18~25h；
Wherein, the mass fraction of component and each component is as follows in the Chinese medicine additive:
2~4 parts of Radix Notoginseng, 1~1.5 part of Radix Salviae Miltiorrhizae, 5~8 parts of Chinese yam, 10~13 parts of Radix Astragali, 15~25 parts of pueraria lobata, 1~3 part of radix paeoniae rubra,
1~3 part of Rhizoma Atractylodis Macrocephalae, 2~8 parts of cowberry, 15~25 parts of honeysuckle, 10~18 parts of fructus lycii.
2. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as described in claim 1, which is characterized in that institute
Stating seed liquor inoculative proportion is
In formula, WDFor the mass fraction of Chinese medicine additive, WCFor the mass fraction of corncob, WBFor the mass fraction of bean product,
WRFor the mass fraction of sorghum rice and maize and.
3. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as claimed in claim 2, which is characterized in that
In the step 4, the Zengjing Granule time is
In formula, WDFor the mass fraction of Chinese medicine additive, WOFor the other components mass fraction and W for removing Chinese medicine additiveBFor
The mass fraction of bean product, WRAnd, t is Zengjing Granule time, t for the mass fraction of sorghum rice and maizemaxFor Zengjing Granule
Maximum time, tminFor Zengjing Granule minimum time, teFor Zengjing Granule executeaaafunction,For Zengjing Granule time adjustment coefficient, η
For Zengjing Granule executeaaafunction correction coefficient, e is the truth of a matter of natural logrithm.
4. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as described in claim 1, which is characterized in that
In the step 3, the seed liquor preparation process are as follows:
The probiotics strain is added in MRS culture medium with 1% volume ratio, 37 DEG C of static gas wave refrigerators, 12 hours progress bacterium
The recovery of kind；Recovery bacterium solution is inoculated into the MRS culture medium by 10% volume ratio, 37 DEG C of static gas wave refrigerators 12 hours into
The preparation of row probiotics bacterial seed liquor；
The MRS culture medium sterilizes under 115 DEG C, 0.1Mpa 30min after configuring, and natural cooling uses after sterilizing.
5. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as claimed in claim 4, which is characterized in that institute
Component and the mass fraction for stating MRS culture medium are as follows:
20 parts of peptone, 10 parts of beef extract, 5 parts of yeast extract, 4 parts of diammonium hydrogen citrate, 20 parts of glucose, 3.2 parts of Tween 80,
5 parts of sodium acetate, 2 parts of dipotassium hydrogen phosphate, 0.25 part of magnesium sulfate, 0.05 part of manganese sulfate, 18 parts of agar, 1000 parts of distilled water.
6. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as described in claim 1, which is characterized in that
In the step 2, the dry mixing time is 30~50 minutes, described to be stirred for being 80~100 minutes.
7. the breeding method according to any one of claims 1 to 5 that number of live bacteria of probiotics is improved using Chinese medicine additive,
It is characterized in that, in the step 2, the dry mixing time is
In formula, WCFor the mass fraction of corncob, WBFor the mass fraction of bean product, WRFor the mass parts of sorghum rice and maize
Number and T1For dry mixing time, T1_maxFor dry stirring maximum time, T1_minFor dry stirring minimum time, T1_eTo do stirring experience
Time, δ1For dry mixing time correction coefficient, γ1For dry stirring executeaaafunction correction coefficient.
8. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as claimed in claim 7, which is characterized in that institute
It states and is stirred for the time and is
In formula, WCFor the mass fraction of corncob, WBFor the mass fraction of bean product, WRFor the mass parts of sorghum rice and maize
Number and T2To be stirred for time, T2_maxTo be stirred for maximum time, T2_minTo be stirred for minimum time, T2_eTo be stirred for experience
Time, δ2To be stirred for time adjustment coefficient, γ2To be stirred for executeaaafunction correction coefficient.
9. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as claimed in claim 8, which is characterized in that δ1
Value is 1.43~1.55, γ1Value is 0.23, δ2Value is 2.35~2.47, γ2Value is 1.13.
10. the breeding method of number of live bacteria of probiotics is improved using Chinese medicine additive as claimed in claim 9, which is characterized in that
Value is that 1.3~1.5, η value is 2.3.
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