CN109563492A - 突变的病毒、其制备方法和应用 - Google Patents

突变的病毒、其制备方法和应用 Download PDF

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CN109563492A
CN109563492A CN201680076047.3A CN201680076047A CN109563492A CN 109563492 A CN109563492 A CN 109563492A CN 201680076047 A CN201680076047 A CN 201680076047A CN 109563492 A CN109563492 A CN 109563492A
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virus
amino acid
trna
mutation
albumen
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CN109563492B (zh
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周德敏
司龙龙
周雪莹
张子威
徐欢
田振宇
张传领
肖苏龙
夏青
张礼和
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Peking University
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Abstract

提供一种人源的或其它动物的突变病毒及其制备方法，优选流感病毒。该方法包括使用反向遗传技术在病毒基因组的一个或多个基因的自身终止密码子上游引入密码子UAG。还提供了该突变病毒的应用，如作为减毒活疫苗、复制可控的安全病毒模型等的用途。

Description

PCT国内申请，说明书已公开。

Claims (39)

  1. PCT国内申请，权利要求书已公开。
CN201680076047.3A 2015-12-31 2016-08-02 突变的病毒、其制备方法和应用 Active CN109563492B (zh)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113502274A (zh) * 2021-06-21 2021-10-15 武汉大学 一种表达人***瘤病毒衣壳蛋白l2的重组流感病毒株及其制备方法与应用
WO2024040453A1 (zh) * 2022-08-24 2024-02-29 北京大学宁波海洋药物研究院 突变的流感病毒、药物组合物及用途

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112725282A (zh) * 2016-01-27 2021-04-30 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN109295100A (zh) * 2017-07-25 2019-02-01 北京大学 携带正交tRNA/氨酰tRNA合成酶的稳定细胞系的构建
CN107964035B (zh) * 2017-12-06 2020-07-24 中国科学院微生物研究所 一种用于流感病毒的有限复制型黏膜免疫疫苗
CN109180680B (zh) * 2018-08-01 2020-05-08 苏州大学 一种紫外光触发交联型近红外分子探针及其制备方法与应用
CN110835633B (zh) * 2018-08-13 2021-10-01 北京大学 利用优化的基因密码子扩展***制备ptc稳定细胞系及应用
CN110846311A (zh) * 2018-08-20 2020-02-28 北京大学 利用抑制性tRNA***制备PTC稳定细胞系及应用
CN109111508B (zh) * 2018-09-19 2021-03-19 天康生物股份有限公司 禽流感病毒血凝素抗原及表达其的cho细胞株、制备方法和疫苗
CN109652384B (zh) * 2019-02-21 2021-10-26 昆明理工大学 一种体外培养戊型肝炎病毒的方法
CN109957550B (zh) * 2019-03-13 2020-10-20 浙江森卫生物医药发展有限公司 流感病毒的拯救方法及其组合物
CN109943536B (zh) * 2019-03-26 2021-09-14 昆明理工大学 一种戊型肝炎病毒的培养方法及其灭活疫苗的制备方法
CN109971888A (zh) * 2019-04-11 2019-07-05 浙江森卫生物医药发展有限公司 一种复制可控型流感病毒的检测方法
CN110042084A (zh) * 2019-04-11 2019-07-23 浙江森卫生物医药发展有限公司 一种活病毒流感疫苗的生产方法以及制剂
JP2022538693A (ja) * 2019-07-05 2022-09-05 龍龍 司 タンパク質加水分解標的ウイルス、その生ワクチン及びその作製方法と用途
CN110743007B (zh) * 2019-09-10 2023-05-30 安徽智飞龙科马生物制药有限公司 一种联合疫苗及其制备方法、应用
CN110845587B (zh) 2019-10-15 2021-06-29 康希诺生物股份公司 一种定点突变的载体蛋白及其在制备疫苗中的用途
CN111533792B (zh) * 2020-07-07 2020-10-16 康希诺生物股份公司 一种定点突变的载体蛋白及其在制备疫苗中的用途
CN115074330B (zh) * 2022-04-19 2023-12-29 中国医学科学院医学生物学研究所 一种基于遗传密码子扩展技术的改造Vero细胞系
CN116904403B (zh) * 2023-09-13 2024-01-09 北京民海生物科技有限公司 一种腮腺炎病毒突变体及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1635905A (zh) * 2001-07-20 2005-07-06 乔治亚大学研究基金会 通过核蛋白在磷酸化位点突变的减弱狂犬病病毒用于抗狂犬病的接种以及在cns中的基因治疗
US20120269849A1 (en) * 2009-10-09 2012-10-25 Eckard Wimmer Attenuated influenza viruses and vaccines
CN102838663A (zh) * 2011-06-23 2012-12-26 北京大学 定点突变和定点修饰的病毒膜蛋白、其制备方法及其应用
CN104592364A (zh) * 2013-10-30 2015-05-06 北京大学 定点突变和定点修饰的腺相关病毒、其制备方法及应用
WO2015100277A2 (en) * 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2128246T3 (da) * 2001-04-19 2014-05-12 Univ California Fremgangsmåder og sammensætninger til fremstilling af ortogonale tRNA-aminoacyl-tRNA-syntetasepar.
MX2010008633A (es) * 2008-02-08 2010-09-30 Scripps Research Inst Ruptura de tolerancia inmunologica con un aminoacido no natural geneticamente codificado.
TR201802361T4 (tr) * 2008-09-26 2018-03-21 Ambrx Inc Doğal olmayan amino asit replikasyonuna bağımlı mikroorganizmalar ve aşılar.
EP2897935B1 (en) * 2012-09-24 2019-02-27 MedImmune Limited Amino acid derivatives
CN102964432B (zh) * 2012-11-22 2014-02-05 中国科学院微生物研究所 抑制a型流感病毒m1蛋白发生磷酸化的方法及其应用
US10322172B2 (en) * 2014-02-10 2019-06-18 Nutech Ventures Live, attenuated vaccines and methods of making and using
MA40773A (fr) * 2014-10-02 2017-08-08 Vertex Pharma Variants du virus influenza a

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1635905A (zh) * 2001-07-20 2005-07-06 乔治亚大学研究基金会 通过核蛋白在磷酸化位点突变的减弱狂犬病病毒用于抗狂犬病的接种以及在cns中的基因治疗
US20120269849A1 (en) * 2009-10-09 2012-10-25 Eckard Wimmer Attenuated influenza viruses and vaccines
CN102838663A (zh) * 2011-06-23 2012-12-26 北京大学 定点突变和定点修饰的病毒膜蛋白、其制备方法及其应用
CN104592364A (zh) * 2013-10-30 2015-05-06 北京大学 定点突变和定点修饰的腺相关病毒、其制备方法及应用
WO2015100277A2 (en) * 2013-12-23 2015-07-02 University Of Rochester Methods and compositions for ribosomal synthesis of macrocyclic peptides

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
YONGXIANG ZHENG ET AL: "Broadening the versatility of lentiviral vectors as a tool in nucleic acid research via genetic code expansion", 《NUCLEIC ACIDS RESEARCH》 *
闻玉梅: "《现代微生物学》", 31 January 1991, 上海医科大学出版社 *
饶洪洋，人民军医出版社: "《病原生物与免疫学基础》", 28 February 2015, 人民军医出版社 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113502274A (zh) * 2021-06-21 2021-10-15 武汉大学 一种表达人***瘤病毒衣壳蛋白l2的重组流感病毒株及其制备方法与应用
WO2024040453A1 (zh) * 2022-08-24 2024-02-29 北京大学宁波海洋药物研究院 突变的流感病毒、药物组合物及用途

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BR112018013168A2 (zh) 2018-12-11
EP3399029A1 (en) 2018-11-07
ZA201804362B (en) 2019-04-24
JP2019500051A (ja) 2019-01-10
IL260258A (en) 2018-07-31
CN109563492B (zh) 2023-05-12
US11510974B2 (en) 2022-11-29
AU2016383201A1 (en) 2018-07-05
EP3399029A4 (en) 2019-07-17
US20200268870A1 (en) 2020-08-27

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