CN109526565A - A kind of Pleurotus eryngii liquid fermentation medium and strain cultivation method and method for planting almond abalone mushroom - Google Patents
A kind of Pleurotus eryngii liquid fermentation medium and strain cultivation method and method for planting almond abalone mushroom Download PDFInfo
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- CN109526565A CN109526565A CN201811524571.XA CN201811524571A CN109526565A CN 109526565 A CN109526565 A CN 109526565A CN 201811524571 A CN201811524571 A CN 201811524571A CN 109526565 A CN109526565 A CN 109526565A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/50—Inoculation of spawn
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Abstract
The invention discloses a kind of Pleurotus eryngii liquid fermentation medium and strain cultivation method and method for planting almond abalone mushroom.Liquid fermentation medium includes sugar-cane juice, vinasse powder, soy meal, corn flour, peptone, potassium phosphate, magnesium chloride, calcium carbonate and sterile water.Strain cultivation method is to be inoculated into liquid fermentation medium after activating pleurotus eryngii quel strains, and shaking table shaken cultivation 6~8 days is under the conditions of 20~30 DEG C up to liquid spawn.Planting almond abalone mushroom is then cultivation bag is stacked in greenhouse to equipped in the cultivation bag of culture medium, temperature, gas concentration lwevel and intensity of illumination in greenhouse is altered in steps, finished product Pleurotus eryngii can be turned out after a period of time liquid spawn access.Using the liquid fermentation medium and strain cultivation method in the present invention, the diameter of mycelium pellet in strain can be effectively reduced, the density of mycelium pellet is increased.Planting almond abalone mushroom is carried out based on this strain, can significantly improve Pleurotus eryngii culture efficiency.
Description
Technical field
The invention belongs to planting almond abalone mushroom technical fields, and in particular to a kind of Pleurotus eryngii liquid fermentation medium and liquid bacteria
Kind cultural method and method for planting almond abalone mushroom.
Background technique
Pleurotus eryngii is the deep nutrition and health care food liked by people, in recent years due to the swift and violent hair of industrial cultivation technique
Exhibition improves strain quality and production efficiency to shorten strain manufacturing cycle, and domestic and international manufacturer's research and development liquid spawn is used
The solid spawn generally used in the current Pleurotus eryngii production of substitution, and start to apply in actual production.
Pleurotus eryngii liquid strain is that great-hearted Pleurotus eryngii mycelium is produced by liquid fermentation process.Currently, the country is still
Do not promulgate that unified national standard comes the quality and quality of specification pleurotus eryngii liquid strain, only people in actual application
It summarizes application effect and has worked out some region standards, clearly stipulate that qualified edible fungi liquid strain is answered in these standards
It is somebody's turn to do " visible distinctive hypha form, spherical and plexi mycelium are largely distributed, and mycelia is sturdy, and plasm is evenly distributed in mycelia "
" bacterium solution is slightly sticky thick, have a large amount of sheets or it is spherical it is mycelium suspended, be evenly distributed ", that is to say, that excellent liquid spawn mycelium pellet
Density is high, diameter is small, is evenly distributed；Therefore the physical characteristics such as size, density, uniformity of Pleurotus eryngii liquid fermentation mycelium pellet are
The crucial Con trolling index of high-quality liquid spawn.
Domestic research report and patented technology in relation to Pleurotus eryngii Liquid Culture concentrates on the optimization side of zymotechnique substantially
Face mainly adds the research for improving zymophyte pompon physical characteristic by the method being mechanically pulverized or in liquid medium
Add bead, little spring by way of breaing up mycelium pellet to reduce fermented hypha bulb diameter, increases density and the uniformity.It is mechanical
The method and process of crushing is complicated, and easily infection miscellaneous bacteria causes cultivation to fail；The method of addition bead and little spring is only applicable to
It is almost inoperable to be applied to the production of batch production fermentor for the small-scale production of shake flask fermentation.
Summary of the invention
For the above-mentioned prior art, the present invention provides a kind of pleurotus eryngii liquid strain fermentation medium and utilizes its culture
The method of pleurotus eryngii liquid strain can effectively reduce mycelia bulb diameter, increase mycelium pellet density.The liquid prepared is utilized simultaneously
Body strain carries out planting almond abalone mushroom, can significantly improve Pleurotus eryngii culture efficiency.
1. Pleurotus eryngii fluid nutrient medium and its preparation
(1) raw material preparation:
Sugar-cane juice: taking fresh cane and squeeze the juice, and filters the juice squeezed out, obtains sugar-cane juice；
Vinasse powder: the vinasse after taking grain to make wine are dried to water content lower than 10%, are then mixed into 3% (quality
Score) left and right pulverized limestone, mixture is worn into 40~50 mesh powders, obtains vinasse powder；
Soy meal: can provide or buy ready-made soy meal for oneself, and providing for oneself is that defatted soybean is ground into 5~20 μm
Powder；
Corn flour: can provide or buy ready-made corn flour for oneself, and providing for oneself is the corn flour that water content is lower than to 10%
It is broken into 5~20 μm of powder；
Peptone, potassium phosphate, magnesium chloride, calcium carbonate etc. buy finished product.
(2) prepared by fluid nutrient medium:
Weighing ready raw material, each raw material is respectively as follows: 3~6 parts of sugar-cane juice by weight, and 1~3 part of vinasse powder,
1~3 part of soy meal, 1~3 part of corn flour, 0.5~1.5 part of peptone, 0.5~1.5 part of potassium phosphate, 0.5~1.5 part of magnesium chloride,
0.5~1.5 part of calcium carbonate；The raw material weighed up is added in 80~85 parts of sterile water, is put into high pressure sterilization after mixing evenly
It sterilizes in pot, obtains fluid nutrient medium, and the pH value of fluid nutrient medium is adjusted to 6.5~7.5 with acid-base modifier.Through
Many experiments discovery is crossed, the optimal set of fluid nutrient medium becomes: 5 parts of sugar-cane juice, 2 parts of vinasse powder, 2 parts of soy meal, corn flour 2
Part, 1 part of peptone, 1 part of potassium phosphate, 1 part of magnesium chloride, 1 part of calcium carbonate, 85 parts of sterile water；The optimal ph of fluid nutrient medium is
7.0。
Be added with sugar-cane juice in fluid nutrient medium of the invention, contain various saccharides in sugar-cane juice, as sucrose, glucose and
In addition to this fructose etc. further includes a variety of amino acid and vitamin, can not only provide carbon source and nutrients for the production of strain
Matter, and the viscosity of adjustable fermentation liquid, compatibility, stability, adsorptivity and biocidal property, can significantly improve mycelium pellet
Distribution uniformity in fermentation liquid, mycelium pellet density is big in the liquid spawn turned out, and diameter is small, is evenly distributed.Liquid training
The vinasse supported in base contain a large amount of celluloses, can provide a large amount of carbon sources and nutriment for the fermentation of abalone mushroom strain；And fiber
Plain structure-rich, uniformly, environmental suitability ability can be provided for the subsequent cultivation of Pleurotus eryngii.
2. the culture of pleurotus eryngii liquid strain
After preparing Pleurotus eryngii fluid nutrient medium, based on this, the culture of pleurotus eryngii liquid strain has been carried out.Liquid
The cultural method of strain the following steps are included:
(1) actication of culture: the slant strains of preservation are activated by plating medium；Strain can be Pleurotus eryngii CICC
14011, Pleurotus eryngii ACCC 51678 or Pleurotus eryngii ACCC 51330；
(2) pleurotus eryngii quel strains after overactivation are inoculated into liquid fermentation medium by the inoculum concentration of 6%~10wt%
In, shaking table shaken cultivation 6~8 days, obtain pleurotus eryngii liquid strain under the conditions of 20~30 DEG C；The revolving speed of shaking table is in incubation
180~220r/m.It is found after a series of screening of condition of culture, the optimal culture conditions of liquid spawn are as follows: pleurotus eryngii quel strains
Inoculum concentration in liquid fermentation medium is 8wt%；Cultivation temperature is 25 DEG C, and incubation time is 7 days.
3. method for planting almond abalone mushroom
The present invention has carried out the cultivation of Pleurotus eryngii after successfully turning out Pleurotus eryngii liquid-liquid strain on this basis,
In the present invention Pleurotus eryngii cultural method the following steps are included:
S1: in the greenhouse equipped with temperature control system and carbon-dioxide generator (in such as patent CN205337041U
Disclosed automated greenhouse greenhouse) build Pleurotus eryngii plantation frame, plantation frame is divided into 3~4 layers with partition, and every layer height is 0.5~
0.7m；Spacing between every two plantation frame is 0.7~1.2m；
S2: by the liquid spawn access turned out using pleurotus eryngii liquid strain cultural method in the present invention equipped with culture medium
Cultivation bag in, cultivate bag in liquid spawn and culture medium volume ratio 1:8~10, then by cultivate bag be successively stacked to plantation
On frame；Controlling the temperature in greenhouse is 25~27 DEG C, and gas concentration lwevel is 0.03%~0.05%, continues 2~4 days,
Cultivation bag is covered with to Pleurotus eryngii mycelia；
S3: after Pleurotus eryngii mycelia covers with and cultivates bag, the temperature in greenhouse is reduced to 20~22 DEG C, keeps titanium dioxide
Concentration of carbon is constant, continues 1~2 day；
S4: it after S3 is handled, opens and cultivates bag sack, and the temperature in greenhouse is risen to 25 DEG C, control illumination
Intensity is in 50Lx hereinafter, continuing 2~3 days, until former base is long to 1~2cm；
S5: after former base is grown, control greenhouse in temperature be 12~18 DEG C, gas concentration lwevel be 0.3%~
0.5%, intensity of illumination is 600~800Lx, continues 15~18 days, until Pleurotus eryngii is mature.
Culture medium used that filled in bag of cultivating of the invention includes the component that following quality is divided: 20~30 parts of bagasse, wine
10~15 parts, 1~3 part of clover, 0.5~1.5 part of potassium dihydrogen phosphate, 0.5~1.5 part of magnesium sulfate of grain.Wherein, bagasse is sugarcane
Remaining residue after juicing, partial size are 1~2mm；Vinasse are remaining vinasse after grain wine brewing, and partial size is 1~2mm；Lucerne
Mu is the alfalfa stem and leaf that water content is 50~60%, and partial size is 1~2mm.Culture medium is the preparation method comprises the following steps: weigh corresponding mass
Component uniformly mixes them, controls whole water content in 50%~60% range, and adjust reagent for its pH value tune with pH
It saves to 5.5~6.5.
4. the detection method of fluid present invention strain primary quality measure
(1) Pleurotus eryngii mycelium pellet density quantification method: taking 1ml culture solution, and sterile saline is diluted to 10ml, mixes, takes
1mL dilution is placed in plate, expansion, is served as a contrast black background paper under plate, is counted.
(2) Pleurotus eryngii mycelium pellet dry weight method (biomass): 100ml culture solution uniformly is taken, through 80 mesh net filtrations, use is sterile
Brine, until cleaning solution is clarified.Mycelium pellet is placed on the qualitative filter paper of constant weight, 60 DEG C dry to constant weight after weigh.
(3) mycelium pellet measuring diameter: taking mycelium pellet 20 at random, be arranged in a straight line, survey its length with vernier caliper, calculates
The average diameter (mm) of 20 mycelium pellets is obtained, is repeated 3 times.
The beneficial effects of the present invention are:
Fluid nutrient medium each component source in the present invention is easy, and the mycelia bulb diameter turned out obviously becomes smaller, and bacterium solution is close
Degree improves, and even suspension.The pleurotus eryngii liquid strain prepared using this method is healthy and strong, is inoculated with growth vigor after solid medium
Better than conventional method liquid spawn, the speed of growth is fast, and mycelia is sturdy, and hyphal cell plasm is full uniform.
Specific embodiment
Below with reference to embodiment, specific embodiments of the present invention will be described in detail.
Embodiment one
A kind of Pleurotus eryngii liquid fermentation medium, is made by the following method: by sugar-cane juice 50g, vinasse powder 20g,
Soy meal 20g, corn flour 20g, peptone 10g, potassium phosphate 10g, magnesium chloride 10g and calcium carbonate 10g are mixed with sterile water 850g,
After high pressure sterilization, pH value is adjusted to 7.0.
Embodiment two
A kind of Pleurotus eryngii liquid fermentation medium, is made by the following method: by sugar-cane juice 30g, vinasse powder 30g,
Soy meal 30g, corn flour 30g, peptone 5g, potassium phosphate 15g, magnesium chloride 5g and calcium carbonate 15g are mixed with sterile water 840g, high
After pressure sterilizing, pH value is adjusted to 6.5.
Embodiment three
A kind of Pleurotus eryngii liquid fermentation medium, is made by the following method: by sugar-cane juice 60g, vinasse powder 10g,
Soy meal 30g, corn flour 10g, peptone 15g, potassium phosphate 5g, magnesium chloride 15g and calcium carbonate 5g are mixed with sterile water 850g, high
After pressure sterilizing, pH value is adjusted to 7.5.
Example IV
A kind of pleurotus eryngii liquid strain cultural method, comprising the following steps:
(1) 14011 inclined-plane bacterium of the Pleurotus eryngii CICC of preservation is activated by plating medium；
(2) liquid fermentation being inoculated into the pleurotus eryngii quel strains after overactivation by the inoculum concentration of 8wt% in embodiment one
In culture medium, shaking table shaken cultivation 7 days, obtain pleurotus eryngii liquid strain under the conditions of 25 DEG C；The revolving speed of shaking table is in incubation
200r/m。
Embodiment five
A kind of pleurotus eryngii liquid strain cultural method, comprising the following steps:
(1) 51678 inclined-plane bacterium of the Pleurotus eryngii ACCC of preservation is activated by plating medium；
(2) liquid fermentation being inoculated into the pleurotus eryngii quel strains after overactivation by the inoculum concentration of 6wt% in embodiment two
In culture medium, shaking table shaken cultivation 6 days, obtain pleurotus eryngii liquid strain under the conditions of 30 DEG C；The revolving speed of shaking table is in incubation
180r/m。
Embodiment six
A kind of pleurotus eryngii liquid strain cultural method, comprising the following steps:
(1) 51330 inclined-plane bacterium of the Pleurotus eryngii ACCC of preservation is activated by plating medium；
(2) liquid fermentation being inoculated into the pleurotus eryngii quel strains after overactivation by the inoculum concentration of 10wt% in embodiment three
In culture medium, shaking table shaken cultivation 6 days, obtain pleurotus eryngii liquid strain under the conditions of 30 DEG C；The revolving speed of shaking table is in incubation
180r/m。
Comparative example one
A kind of pleurotus eryngii liquid strain cultural method, comprising the following steps:
(1) 14011 inclined-plane bacterium of the Pleurotus eryngii CICC of preservation is activated by plating medium；
(2) pleurotus eryngii quel strains after overactivation are inoculated into comparison liquid fermentation medium by the inoculum concentration of 8wt%,
Shaking table shaken cultivation 6 days under the conditions of 30 DEG C, obtain pleurotus eryngii liquid strain, and the revolving speed of shaking table is 180r/m in incubation；Often
It includes: vinasse powder 50g, soy meal 20g, corn flour 20g, peptone 10g, potassium phosphate that 1000g, which compares liquid fermentation medium,
10g, magnesium chloride 10g, calcium carbonate 10g, sterile water 870g.
Comparative example two
A kind of pleurotus eryngii liquid strain cultural method, comprising the following steps:
(1) 14011 inclined-plane bacterium of the Pleurotus eryngii CICC of preservation is activated by plating medium；
(2) pleurotus eryngii quel strains after overactivation are inoculated into comparison liquid fermentation medium by the inoculum concentration of 8wt%,
Shaking table shaken cultivation 6 days under the conditions of 30 DEG C, obtain pleurotus eryngii liquid strain, and the revolving speed of shaking table is 180r/m in incubation；Often
It includes: sugar-cane juice 50g, soy meal 20g, corn flour 20g, peptone 10g, potassium phosphate that 1000g, which compares liquid fermentation medium,
10g, magnesium chloride 10g, calcium carbonate 10g, sterile water 870g.
Comparative example three
A kind of pleurotus eryngii liquid strain cultural method, comprising the following steps:
(1) 14011 inclined-plane bacterium of the Pleurotus eryngii CICC of preservation is activated by plating medium；
(2) pleurotus eryngii quel strains after overactivation are inoculated into comparison liquid fermentation medium by the inoculum concentration of 8wt%,
Shaking table shaken cultivation 6 days under the conditions of 30 DEG C, obtain pleurotus eryngii liquid strain, and the revolving speed of shaking table is 180r/m in incubation；Often
It includes: glucose 40g, soybean powder 30g, corn flour 30g, potassium dihydrogen phosphate 5g, magnesium sulfate that 1000g, which compares liquid fermentation medium,
10g, vitamin 5g, gelatin 10g, sterile water 870g.
Interpretation of result
According to the main indicator detection method detection example IV~embodiment six and comparative example recorded in summary of the invention
Density, dry weight and the diameter of mycelium pellet, are as a result listed in table 1 in the liquid spawn of one~comparative example three after fermentation.
Mycelium pellet quality index in 1 liquid spawn of table
As can be seen from the table, using in the present invention fluid nutrient medium and the liquid spawn turned out of cultural method in bacterium
The density of pompon is larger, and dry weight is higher, and diameter is smaller, can prepare distribution without adding bead etc. into liquid spawn
Even and partial size composite demand strain, is conducive to the extensive cultivating and growing of Pleurotus eryngii industrial.
One embodiment one of comparative example is compared, and sugar-cane juice has been lacked in fluid nutrient medium, among obtained liquid spawn bacterium
Pompon density is low, is relatively large in diameter, it may be possible to because the compatibility and stability of fluid nutrient medium reduce after lacking sugar-cane juice,
It is unfavorable for mycelium pellet to spread in Liquid Culture, mycelium pellet is mutually assembled, and the diameter of single mycelium pellet is caused to increase.Comparative example two
Vinasse powder is lacked compared with embodiment one, in fluid nutrient medium, the carbon source and nutriment in culture medium are reduced, and are unfavorable for
The formation and growth of mycelium pellet, therefore, mycelium pellet density and dry weight are respectively less than example IV.Comparative example is third is that using existing liquid
Body culture medium carries out strain cultivation, compared with example IV, or has certain gap.To sum up, using in the present invention
Fluid nutrient medium strain cultivation method can prepare a kind of Pleurotus eryngii liquid bacteria of suitable large-scale production really
Kind.
Embodiment seven
A kind of method for planting almond abalone mushroom, comprising the following steps:
S1: building Pleurotus eryngii plantation frame in the greenhouse equipped with temperature control system and carbon-dioxide generator, described
Plantation frame is divided into 3~4 layers with partition, and every layer height is 0.5~0.7m；
S2: by the liquid spawn access turned out using pleurotus eryngii liquid strain cultural method in the present invention equipped with culture medium
Cultivation bag in, the volume ratio 1:9 of liquid spawn and culture medium, then by cultivate bag be successively stacked in plantation frame；Control temperature
Temperature in the greenhouse of room is 26 DEG C, and gas concentration lwevel 0.04% continues 3 days, until Pleurotus eryngii mycelia covers with cultivation bag；It cultivates
Culture medium in bag includes: 25 parts of bagasse by weight, and 12 parts of vinasse, 2 parts of clover, 1 part of potassium dihydrogen phosphate, magnesium sulfate 1
Part；
S3: after Pleurotus eryngii mycelia covers with and cultivates bag, the temperature in greenhouse is reduced to 21 DEG C, keeps carbon dioxide dense
It spends constant, continues 2 days；
S4: it after S3 is handled, opens and cultivates bag sack, the temperature in greenhouse is risen to 25 DEG C, and control illumination
Intensity is in 50Lx hereinafter, continuing 2 days, until former base is long to 1~2cm；
S5: after former base is grown, the temperature controlled in greenhouse is reduced to 15 DEG C, and gas concentration lwevel 0.4%, illumination is strong
Degree is 700Lx, continues 16 days, until Pleurotus eryngii is mature.
Embodiment eight
A kind of method for planting almond abalone mushroom, comprising the following steps:
S1: building Pleurotus eryngii plantation frame in the greenhouse equipped with temperature control system and carbon-dioxide generator, described
Plantation frame is divided into 3~4 layers with partition, and every layer height is 0.5~0.7m；
S2: by the liquid spawn access turned out using pleurotus eryngii liquid strain cultural method in the present invention equipped with culture medium
Cultivation bag in, the volume ratio 1:8 of liquid spawn and culture medium, then by cultivate bag be successively stacked in plantation frame；Control temperature
Temperature in the greenhouse of room is 25 DEG C, and gas concentration lwevel 0.05% continues 2 days, until Pleurotus eryngii mycelia covers with cultivation bag；It cultivates
Culture medium in bag includes: 30 parts of bagasse by weight, and 10 parts of vinasse, 3 parts of clover, 0.5 part of potassium dihydrogen phosphate, magnesium sulfate
1.5 part；
S3: after Pleurotus eryngii mycelia covers with and cultivates bag, the temperature in greenhouse is reduced to 20 DEG C, keeps carbon dioxide dense
It spends constant, continues 1 day；
S4: it after S3 is handled, opens and cultivates bag sack, and the temperature in greenhouse is risen to 25 DEG C, control illumination
Intensity is in 50Lx hereinafter, continuing 3 days, until former base is long to 1~2cm；
S5: after former base is grown, the temperature controlled in greenhouse is reduced to 12 DEG C, and gas concentration lwevel 0.3%, illumination is strong
Degree is 800Lx, continues 16 days, until Pleurotus eryngii is mature.
Embodiment nine
A kind of method for planting almond abalone mushroom, comprising the following steps:
S1: building Pleurotus eryngii plantation frame in the greenhouse equipped with temperature control system and carbon-dioxide generator, described
Plantation frame is divided into 3~4 layers with partition, and every layer height is 0.5~0.7m；
S2: by the liquid spawn access turned out using pleurotus eryngii liquid strain cultural method in the present invention equipped with culture medium
Cultivation bag in, the volume ratio 1:10 of liquid spawn and culture medium, then by cultivate bag be successively stacked in plantation frame；Control temperature
Temperature in the greenhouse of room is 27 DEG C, and gas concentration lwevel 0.03% continues 4 days, until Pleurotus eryngii is sprouted completely；It cultivates in bag
Culture medium includes: 20 parts of bagasse, 15 parts of vinasse, 1 part of clover, 1.5 parts of potassium dihydrogen phosphate, 0.5 part of magnesium sulfate by weight；
S3: after Pleurotus eryngii mycelia covers with and cultivates bag, the temperature in greenhouse is reduced to 20~22 DEG C, keeps titanium dioxide
Concentration of carbon is constant, continues 1 day；
S4: it after S3 is handled, opens and cultivates bag sack, and the temperature in greenhouse is risen to 25 DEG C, control illumination
Intensity is in 50Lx hereinafter, continuing 3 days, until former base is long to 1~2cm；
S5: after former base is grown, the temperature controlled in greenhouse is reduced to 18 DEG C, and gas concentration lwevel 0.5%, illumination is strong
Degree is 600Lx, continues 16 days, until Pleurotus eryngii is mature.
Although be described in detail to a specific embodiment of the invention in conjunction with the embodiments, should not be construed as to this
The restriction of the protection scope of patent.In range described by claims, those skilled in the art are without creative work
The various modifications and deformation that can make still belong to the protection scope of this patent.
Claims (10)
1. a kind of Pleurotus eryngii liquid fermentation medium, which is characterized in that the component including following mass parts: 3~6 parts of sugar-cane juice,
1~3 part of vinasse powder, 1~3 part of soy meal, 1~3 part of corn flour, 0.5~1.5 part of peptone, 0.5~1.5 part of potassium phosphate, chlorine
Change 0.5~1.5 part of magnesium, 0.5~1.5 part of calcium carbonate, 80~85 parts of sterile water；The pH value of the fluid nutrient medium be 6.5~
7.5。
2. Pleurotus eryngii liquid fermentation medium according to claim 1, which is characterized in that the group including following mass parts
Point: 5 parts of sugar-cane juice, 2 parts of vinasse powder, 2 parts of soy meal, 2 parts of corn flour, 1 part of peptone, 1 part of potassium phosphate, 1 part of magnesium chloride, carbon
1 part of sour calcium, 85 parts of sterile water；The pH value of the fluid nutrient medium is 7.0.
3. using the method for the described in any item fluid nutrient medium culture pleurotus eryngii liquid strains of claim 1~2, feature exists
In: it is inoculated into liquid fermentation medium after the pleurotus eryngii quel strains of preservation are activated by the inoculum concentration of 6%~10wt%, 20~
Shaking table shaken cultivation 6~8 days, obtain pleurotus eryngii liquid strain under the conditions of 30 DEG C；In incubation the revolving speed of shaking table be 180~
220r/m。
4. according to the method described in claim 3, it is characterized by: the pleurotus eryngii quel strains connecing in liquid fermentation medium
Kind amount is 8wt%；Cultivation temperature is 25 DEG C, and incubation time is 7 days.
5. the method according to claim 3 or 4, it is characterised in that: the pleurotus eryngii quel strains be Pleurotus eryngii CICC 14011,
Pleurotus eryngii ACCC 51678 or Pleurotus eryngii ACCC 51330.
6. a kind of method for planting almond abalone mushroom, which comprises the following steps:
S1: Pleurotus eryngii plantation frame, the plantation are built in the greenhouse equipped with temperature control system and carbon-dioxide generator
Frame is divided into 3~4 layers with partition, and every layer height is 0.5~0.7m；
S2: the liquid spawn prepared with any one of claim 3~4 the method is accessed into the cultivation bag equipped with culture medium
In, then volume ratio 1:8~10 of liquid spawn and culture medium will be cultivated bag and are successively stacked in plantation frame；It is big to control greenhouse
Temperature in canopy is 25~27 DEG C, and gas concentration lwevel is 0.03%~0.05%, continues 2~4 days, until Pleurotus eryngii mycelia is covered with
Cultivate bag；
S3: after Pleurotus eryngii mycelia covers with and cultivates bag, the temperature in greenhouse is reduced to 20~22 DEG C, keeps carbon dioxide dense
It spends constant, continues 1~2 day；
S4: it after S3 is handled, opens and cultivates bag sack, and the temperature in greenhouse is risen to 25 DEG C, control intensity of illumination
In 50Lx hereinafter, continuing 2~3 days, until former base is long to 1~2cm；
S5: after former base is grown, controlling the temperature in greenhouse is 12~18 DEG C, and gas concentration lwevel is 0.3%~0.5%,
Intensity of illumination is 600~800Lx, continues 15~18 days, until Pleurotus eryngii is mature.
7. method for planting almond abalone mushroom according to claim 6, it is characterised in that: cultivate the body strain in bag and culture in S2
The volume ratio 1:9 of base；Temperature in greenhouse is 26 DEG C, gas concentration lwevel 0.04%.
8. method for planting almond abalone mushroom according to claim 6, it is characterised in that: the temperature in S5 medium temperature chamber greenhouse is 15
DEG C, gas concentration lwevel 0.4%, intensity of illumination 700Lx.
9. method for planting almond abalone mushroom according to claim 6, which is characterized in that the culture medium cultivated in bag includes following matter
The component of amount point: 20~30 parts of bagasse, 10~15 parts of vinasse, 1~3 part of clover, 0.5~1.5 part of potassium dihydrogen phosphate, magnesium sulfate
0.5~1.5 part.
10. method for planting almond abalone mushroom according to claim 9, which is characterized in that the culture medium cultivated in bag includes following
The component of quality point: 25 parts of bagasse, 12 parts of vinasse, 2 parts of clover, 1 part of potassium dihydrogen phosphate, 1 part of magnesium sulfate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811524571.XA CN109526565A (en) | 2018-12-13 | 2018-12-13 | A kind of Pleurotus eryngii liquid fermentation medium and strain cultivation method and method for planting almond abalone mushroom |
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| CN113973646A (en) * | 2021-07-28 | 2022-01-28 | 盐城工学院 | Culture medium and culture method for improving yield of pleurotus eryngii |
| CN114532154A (en) * | 2022-03-11 | 2022-05-27 | 南京吾悦农业科技有限公司 | Method for producing selenium-rich pleurotus eryngii strains through liquid fermentation |
| CN115005009A (en) * | 2022-04-25 | 2022-09-06 | 南京吾悦农业科技有限公司 | Pleurotus eryngii solid strain and preparation method thereof |
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