CN107867934A - A kind of culture medium for being used for true pleurotus cornucopiae and white beech mushroom and its preparation method and application - Google Patents
A kind of culture medium for being used for true pleurotus cornucopiae and white beech mushroom and its preparation method and application Download PDFInfo
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- CN107867934A CN107867934A CN201711207921.5A CN201711207921A CN107867934A CN 107867934 A CN107867934 A CN 107867934A CN 201711207921 A CN201711207921 A CN 201711207921A CN 107867934 A CN107867934 A CN 107867934A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
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Abstract
The invention belongs to the technical field of cultivation of edible fungus cluster, and in particular to a kind of for culture medium of true pleurotus cornucopiae and white beech mushroom and its preparation method and application, the culture medium includes the component of following parts by weight：Component A culture medium inserts, B component culture medium fuel, component C：PH adjusting agent compound；The present invention discloses a kind of preparation method of culture medium and using the true pleurotus cornucopiae of the medium culture in the method for white beech mushroom.The culture medium of the present invention have raw material sources are extensive, packed density uniformly, good permeability, nutritious, the healthy and strong activity of mycelial growth is good, cultivation cycle shortening, cultivation effect is good, low cost and other advantages；The preparation method of the culture medium is simple, easy to operate；True pleurotus cornucopiae and white beech mushroom provided by the invention support the advantages that cultural method is simple, and cultivation period is short, and fruiting is neat, yield is high, fructification quality is good, storability is good, and green, harmless.
Description
Technical field
The invention belongs to the technical field of cultivation of edible fungus cluster, and in particular to a kind of culture for true pleurotus cornucopiae and white beech mushroom
Base and its preparation method and application.
Background technology
True pleurotus cornucopiae (Hypsizygus marmoreus (Peck) H.E.Bigelow), also known as beautiful gill fungus, spot jade gill fungus, lotus leaf from
Pleat umbrella.A variety of necessary amino acid are included in the nutritional ingredient of true pleurotus cornucopiae, its lysine and arginine content are higher than common mushroom class, mouth
Feel good, there is diuresis, invigorating the spleen and other effects.The production of true pleurotus cornucopiae is all by being inoculated with culture medium, turns out mycelia, most
The production technology of harvest product is carried out eventually, and true pleurotus cornucopiae is slow-growing.
White beech mushroom belongs to Agaricales, Tricholomataceae, white mushroom category；It is a kind of Rare edible fungus.White beech mushroom is more general containing protein
Vegetables are higher, it is necessary to which amino acid ratio is suitable, also containing the human body necessary material such as various trace elements, there is good health care to make
With.
Chinese patent application 201410789939.0 discloses a kind of cultivation white beech mushroom culture medium, the culture medium solid
Part is made using the raw material of following percentage by weight：Agricultural crop straw 50%~75%, weed tree sawdust 15%~30%, wheat bran
5%~15%, corn flour 2%~5%, animal excreta 1%~5%, gypsum 1%, lime 1%, wherein the agricultural crop straw is adopted
With Wheat Straw and soybean stalk, graininess is processed into, the weed tree sawdust uses the granular thick wood chip of broad leaf tree.The patent of invention
The cultivation white beech mushroom culture medium of the scheme of application, the primary raw material of use is agricultural crop straw, is had safe and environmental protection excellent
Point, but its nutrient is not comprehensive, influences the quality of white beech mushroom.
Chinese patent application 201510636011.3 discloses a kind of system of 1. culture mediums for the true pleurotus cornucopiae of Liquid Culture
Preparation Method, comprise the following steps：1st step, by weight, by 8~16 parts of analysis for soybean powder, 10~20 parts of glucose, cellulose 6~
12 parts, 1~3 part of vitamin, 0.5~1 part of sodium citrate, 0.3~0.6 part of sodium acetate, 0.2~0.4 part of yeast extract, anti-butylene two
0.2~0.4 part of acid, 0.3~0.6 part of peptone, 0.2~0.3 part of malt extract, 0.2~0.4 part of casein, magnesium sulfate 0.2~0.3
Part, 0.1~0.3 part of dipotassium hydrogen phosphate, 0.1~0.3 part of calcium carbonate, 100~140 parts of distilled water, it is well mixed；2nd step, regulation
The pH of mixed liquor obtained by 1st step；3rd step, the liquid being prepared in the 2nd step are sterilized again, you can.This application is provided
True pleurotus cornucopiae culture medium be a kind of fluid nutrient medium, the content of each composition can be controlled well, be effectively facilitated true pleurotus cornucopiae
Growth；But the culture medium each component is higher by much compared to the component of the true pleurotus cornucopiae culture medium of tradition, cost, thus it is difficult to popularization and makes
With.
Chinese patent application 201510802422.5 discloses a kind of true pleurotus cornucopiae strain culture medium and preparation method thereof, described
The percentage by weight of Spawn incubation based formulas is：Wood chip 20-25%, rice bran 10-15%, water 65%, the wood chip, rice bran weight
Percentage is the percentage of dry weight weight, and the preparation of the true pleurotus cornucopiae strain culture medium includes ratio according to formula ratio by poplar
Wood chip, pine wood chip, rice bran are added in spice pot, and stirring 30-40 minutes are well mixed；Then the water of formula ratio is added, after
It is continuous to stir.The prices of raw materials of the bacterium culture medium of this application are cheap, are easy to buy, and storage is convenient, and prepared by culture medium
Method is easy；The true pleurotus cornucopiae strain cultivated using this method is with short production cycle, and mycelium growth vigor is vigorous, energetic, is inoculated into cultivation
After material, field planting is fast, and pollution rate is low, non-aging.But the Component comparison of the culture medium is single, thus nutrition is not comprehensive enough, use
Easily there is the shortcomings of quality is bad, and yield is not high in the true pleurotus cornucopiae that the medium culture goes out.
The content of the invention
It is an object of the invention to overcome problems of the prior art, there is provided a kind of for true pleurotus cornucopiae and white beech mushroom
Culture medium and its preparation method and application.The culture medium have raw material sources are extensive, packed density uniformly, good permeability, nutrition
Enrich, the healthy and strong activity of mycelial growth is good, cultivation cycle shortens, cultivation effect is good, low cost and other advantages；The preparation side of the culture medium
Method is simple, easy to operate；True pleurotus cornucopiae provided by the invention and the foster cultural method of white beech mushroom are simple, and cultivation period is short, and fruiting is neat, production
The advantages that amount is high, fructification quality is good, storability is good, and it is green, harmless.
The technical scheme is that：A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the group of following parts by weight
Point：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
PH adjusting agent 0.1-2 parts,
Water 0.3-8 parts.
The culture medium can be not only used for the strain that the strain of Liquid Culture can be used for solid culture again.
Further, the culture medium for true pleurotus cornucopiae and white beech mushroom, the component of following parts by weight is included：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
PH adjusting agent 0.1-2 parts,
Water 0.3-8 parts.
Further, the culture medium for true pleurotus cornucopiae and white beech mushroom, the component of following parts by weight is included：Component A:
Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
0.7 part of pH adjusting agent,
2 parts of water.
Further, pH adjusting agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
Further, the particle diameter of the corncob is 3-6mm.
It is higher that the ratio of culture medium is accounted for due to corncob, therefore, the size of its particle diameter has emphatically for the performance of culture medium
The influence wanted.When particle diameter is too big, bottle weight can be caused partially light, charge level out-of-flatness when causing mycelium stimulation, be unfavorable for true pleurotus cornucopiae and nutrition is inhaled
Receive；If particle diameter is too small, culture based structures can be caused excessively fine and close, the poor air permeability of culture medium, be unfavorable for the life of true pleurotus cornucopiae
It is long.
Further, the particle diameter of the wood chip, jowar shell, millet shell and cotton seed hulls is 1-3mm.
The granular size of the raw materials such as wood chip, jowar shell, millet shell and cotton seed hulls of the present invention is moderate, on the one hand former
It can be sufficiently mixed between material, ensure that uniform culture medium packed density in bottling, good permeability, formula material nutrient distribution are equal
Weighing apparatus, facilitates the absorption of true pleurotus cornucopiae and white beech mushroom root system；On the other hand, suitable granular size, culture medium can not only be improved
Water content can improve gas permeability again.
A kind of preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom, comprises the following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be stirred
Uniformly, control time, culture medium inserts mixture a is obtained；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, another side stirring, while spraying into the pH adjusting agent compound, the water content for controlling pH adjusting agent compound is 60-
70%th, pH value 6.0-8.0, mixture b is obtained.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：116-125 DEG C of temperature, pressure 0.080-0.135MPa, time 80-90min, are then cooled to less than 25 DEG C, are used for
The culture medium of true pleurotus cornucopiae and white beech mushroom.
Further, the aperture of punching be 13.5-15mm in culture medium, each sky at intervals of 8-10mm, hole and bottle wall
Distance be 8-10mm.
The culture medium inserts of component A, corncob, wood chip, cotton seed hulls, sorghum husk and millet shell have water imbibition slowly, simultaneously
It is not easy to inhale permeable general character；There is the halfway risk of sterilizing to be planted so as to ultimately result in if when culture medium raw material can not drench water
Training failure.
The culture medium fuel of B component, rice bran, soybean skin, wheat skin, bean cake powder, corn flour, which have, easily inhales that permeable, nutrition is rich
Richness, is the nutrient source that microorganism is liked, easily fermentative acidification goes bad after wet water, is unfavorable for the growth of hypha of edible fungus.
The present invention is classified as component A culture medium inserts, B well according to true pleurotus cornucopiae and white beech mushroom medium feature
Component culture medium fuel, component C medium pH conditioning agent, culture medium preparation method emphasis of the present invention is secondary agitation
Technique --- culture medium inserts adds adds culture medium fuel after the pre- wet fermentation expansion process of water in the state being kept stirring for, most
Medium pH conditioning agent is sprayed into afterwards to be well mixed.The culture medium prepared through Secondary mixing, highly effective improvement above mentioned problem
Difficult point.
Further, in step 1, the pre- wet fermentation expansion process includes weighing component A raw material in proportion, adds suitable
Water is measured, it is 1-2 hours to control initial water content 65-75%, pH value 6.0-8.0,20-25 DEG C of temperature, time, and during which observation is inhaled
Water expansion status.
Further, in step 2, the compound that pH adjusting agent and water are mixed with by spraying into adjusts pH value to 7.
Further, in step 3, described culture bottle volume is 850mL or 1100mL, and wherein 850mL Zai Pei bottle are filled
Mixture b weight is 500-600g, and Zai Pei bottle beat 1-3 hole in surface；1100mL Zai Pei bottle dresses mixture b weight is
650-750g, Zai Pei bottle beat 1-5 hole in surface.
Further, in step 3, sterilising conditions are that culture medium sterilizes 80- at 121 DEG C of temperature, pressure 0.11MPa
85min。
A kind of application for true pleurotus cornucopiae and the culture medium of white beech mushroom, using the above-mentioned training for being used for true pleurotus cornucopiae and white beech mushroom
Support base and cultivate true pleurotus cornucopiae or white beech mushroom, comprise the following steps：
(1) culture medium in true pleurotus cornucopiae and white beech mushroom is taken, to the true pleurotus cornucopiae strain of the inoculation of medium or white jade mushroom
Kind；
(2) the growing environment temperature for controlling strain is 20-25 DEG C, the growing environment relative humidity of strain is 75-80%, bacterium
CO in the growing environment of kind2Concentration≤4000ppm, keep half-light culture, promote strain mycelial growth；
(3) growing state of mycelia is observed, after mycelia covers with bottle and maturation, carries out mycelium stimulation, fruiting；After fruiting, control
The temperature of the growing environment of mushroom is 13-18 DEG C, relative humidity control is 85-100%, CO2Concentration≤4000ppm, illumination it is strong
Spend for 500-1500lux；
(4) when mushroom grows into set size, harvesting, true pleurotus cornucopiae or white beech mushroom are obtained.
Further, take the process of culture medium to be carried out in dust proof workshop in (1), be seeded in hundred grades of laminar flow environments
OK；(3) humid control uses the cold artificial light sources of LED using the advanced stable fluid humidification process of dry fog two, illumination in, and light source is
The combination light of 20% blue light and 80% white light.
The present invention is using using the cold artificial light sources of energy-saving safety LED, the combination light of the white light of 20% blue light+80%, and LED is not
Only be advantageous to the control of fructification commodity shape, moreover it is possible to which, so that light distribution is equal, LED energy-saving and environmental protection in addition are corresponding to reduce
Production cost.
Further, in (3), the opening after fruiting in culture bottle is arranged with horn-like cutting ferrule.
Be arranged horn-like cutting ferrule in bottle opening in the present invention, be advantageous to guide mushroom growth form so that mushroom it is whole
Body appearance looks elegant, it is gratifying, beneficial to fructification it is moulding, convenient harvest.
Further, steamed bun type mycelium stimulation knife mycelium stimulation is used in (3).
The present invention use steamed bun type mycelium stimulation knife mycelium stimulation so that fruiting positioning and directing concentrate, fruiting more neatly, shape more
It is good, while also allow for sporophore growth management.
The present invention culture medium using corncob, wood chip, cotton seed hulls, sorghum husk, millet shell, rice bran, soybean skin, wheat skin,
Bean cake powder, corn flour, medium pH conditioning agent and the plurality of raw materials of water ten are formulated, and realize the optimization of culture based structures, each battalion
Support balanced composition, improve the nutritive value of true pleurotus cornucopiae and white beech mushroom, also improve the fruiting conversion ratio of true pleurotus cornucopiae and white beech mushroom.
The preparation method of traditional culture medium, various raw materials are directly mixed into penetrating moisture, it is easy to cause some component moisture content not
Uniformly, absorption of the mushroom class to nutrition, the preparation method of the culture medium of the application, by the way that the component of different water absorption rates is distinguished are influenceed
Processing, first the slow culture medium inserts (corncob, wood chip, cotton seed hulls, sorghum husk and millet shell) of the larger water suction of water absorption rate is passed through
Cross pre- wet fermentation expansion process, it is ensured that the inside of culture medium inserts keeps more consistent moisture content with outside, then will
Remaining culture medium fuel component mixes, and carries out water spray and is handled with pH value regulation so that the moisture content of each component is consistent, each component
It is evenly distributed, high-temperature sterilization effect is good, and nutriment is easier to the absorption of true pleurotus cornucopiae and white beech mushroom.The present invention applies above-mentioned training
The true pleurotus cornucopiae of base culture or white beech mushroom are supported, the growing environment of true pleurotus cornucopiae or white beech mushroom is controlled, such as temperature, relative humidity, CO2Concentration
With intensity of illumination etc., there is provided true pleurotus cornucopiae or the white beech mushroom optimum growing environment under the culture medium of the present invention, further shorten
The fruiting time and growth time of true pleurotus cornucopiae and white beech mushroom.
Compared with prior art, culture medium of the invention have raw material sources are extensive, packed density uniformly, good permeability,
It is nutritious, the healthy and strong activity of mycelial growth is good, cultivation cycle shortens, cultivation effect is good, low cost and other advantages；The system of the culture medium
Preparation Method is simple, easy to operate；True pleurotus cornucopiae provided by the invention and the foster cultural method of white beech mushroom are simple, and cultivation period is short, and fruiting is whole
Together, the advantages that yield is high, fructification quality is good, storability is good, and it is green, harmless.
Embodiment
Embodiment 1
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
0.7 part of pH adjusting agent,
2 parts of of water
Further, the pH adjusting agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
Wherein, the particle diameter of corncob is 3-6mm；Wood chip, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
A kind of preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom, comprises the following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be stirred
Uniformly, control time, culture medium inserts mixture a is obtained；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, the stirring of another side, while spray into the pH adjusting agent compound, the water content for control pH adjusting agent compound is 67%,
PH value is 6.0, obtains mixture b.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：121 DEG C of temperature, pressure 0.11MPa, time 85min, are then cooled to less than 25 DEG C, obtain being used for true pleurotus cornucopiae and white beech mushroom
Culture medium.
Time used in preparation method whole process is 6.5h.
Wherein, pre- wet fermentation expansion process includes weighing component A raw material in proportion, adds suitable quantity of water, and control initially contains
Water 65%, pH value 6.0,20 DEG C of temperature, time are 1 hour, during which observe water swelling situation.
Wherein, penetrating is mixed with pH adjusting agent in step 2 and the compound of water adjusts pH value to 7.
Wherein, in step 3, culture bottle volume is 850mL, and Zai Pei bottle dress mixtures b weight is 500g, Zai Pei bottle surfaces
Beat 1-3 hole.
Further, the aperture of punching be 13.5-15mm in culture medium, each sky at intervals of 8-10mm, hole and bottle wall
Distance be 8-10mm.
Embodiment 2
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
0.7 part of pH adjusting agent,
2 parts of water.
Further, the pH adjusting agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
Wherein, the particle diameter of corncob is 3-6mm；Wood chip, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
A kind of preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom, comprises the following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be stirred
Uniformly, control time, culture medium inserts mixture a is obtained；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, the stirring of another side, while spray into the pH adjusting agent compound, the water content for control pH adjusting agent compound is 66%,
PH value is 5.5, obtains mixture b.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：116 DEG C of temperature, pressure 0.08MPa, time 80min, are then cooled to less than 25 DEG C, obtain being used for true pleurotus cornucopiae and white beech mushroom
Culture medium.
Time used in preparation method whole process is 6h.
Wherein, pre- wet fermentation expansion process includes weighing component A raw material in proportion, adds suitable quantity of water, and control initially contains
Water 65%, pH value 6.5,20 DEG C of temperature, time are 1 hour, during which observe water swelling situation.
Wherein, penetrating is mixed with pH adjusting agent in step 2 and the compound of water adjusts pH value to 7.
Wherein, in step 3, culture bottle volume is 850mL, and Zai Pei bottle dress mixtures b weight is 500g, Zai Pei bottle surfaces
Beat 1-3 hole.
Further, the aperture of punching be 13.5-15mm in culture medium, each sky at intervals of 8-10mm, hole and bottle wall
Distance be 8-10mm.
Embodiment 3
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
2 parts of pH adjusting agent,
8 parts of water.
Further, the pH adjusting agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
Wherein, the particle diameter of corncob is 3-6mm；Wood chip, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
A kind of preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom, comprises the following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be stirred
Uniformly, control time, culture medium inserts mixture a is obtained；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, the stirring of another side, while spray into the pH adjusting agent compound, the water content for control pH adjusting agent compound is 70%,
PH value is 7.2, obtains mixture b.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：125 DEG C of temperature, pressure 0.135MPa, time 90min, are then cooled to less than 25 DEG C, obtain being used for true pleurotus cornucopiae and white beech mushroom
Culture medium.
Time used in preparation method whole process is 6.3h.
Wherein, pre- wet fermentation expansion process includes weighing component A raw material in proportion, adds suitable quantity of water, and control initially contains
Water 65%, pH value 8,25 DEG C of temperature, time are 1 hour, during which observe water swelling situation.
Wherein, penetrating is mixed with pH adjusting agent in step 2 and the compound of water adjusts pH value to 7.
Wherein, in step 3, culture bottle volume is 1100mL, and Zai Pei bottle dress mixtures b weight is 750g, Zai Pei bottle tables
5 holes are made a call in face.
Further, the aperture of punching be 13.5-15mm in culture medium, each sky at intervals of 8-10mm, hole and bottle wall
Distance be 8-10mm.
Embodiment 4
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
1 part of pH adjusting agent,
3 parts of water.
Further, the pH adjusting agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
Wherein, the particle diameter of corncob is 3-6mm；Wood chip, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
A kind of preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom, comprises the following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be stirred
Uniformly, control time, culture medium inserts mixture a is obtained；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, the stirring of another side, while spray into the pH adjusting agent compound, the water content for control pH adjusting agent compound is 70%,
PH value is 7.2, obtains mixture b.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：120 DEG C of temperature, pressure 0.10MPa, time 80min, are then cooled to less than 25 DEG C, obtain being used for true pleurotus cornucopiae and white beech mushroom
Culture medium.
Time used in preparation method whole process is 6.6h.
Wherein, pre- wet fermentation expansion process includes weighing component A raw material in proportion, adds suitable quantity of water, and control initially contains
Water 65%, pH value 8,25 DEG C of temperature, time are 1 hour, during which observe water swelling situation.
Wherein, penetrating is mixed with pH adjusting agent in step 2 and the compound of water adjusts pH value to 7.
Wherein, in step 3, culture bottle volume is 1100mL, and Zai Pei bottle dress mixtures b weight is 750g, Zai Pei bottle tables
5 holes are made a call in face.
Further, the aperture of punching be 13.5-15mm in culture medium, each sky at intervals of 8-10mm, hole and bottle wall
Distance be 8-10mm.
Embodiment 5
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
1 part of pH adjusting agent,
3 parts of water.
Further, the pH adjusting agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
Wherein, the particle diameter of corncob is 3-6mm；Wood chip, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
A kind of preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom, comprises the following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be stirred
Uniformly, control time, culture medium inserts mixture a is obtained；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, the stirring of another side, while spray into the pH adjusting agent compound, the water content for control pH adjusting agent compound is 68%,
PH value is 7.5, obtains mixture b.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：121 DEG C of temperature, pressure 0.11MPa, time 90min, are then cooled to less than 25 DEG C, obtain being used for true pleurotus cornucopiae and white beech mushroom
Culture medium.
Time used in preparation method whole process is 7h.
Wherein, pre- wet fermentation expansion process includes weighing component A raw material in proportion, adds suitable quantity of water, and control initially contains
Water 65%, pH value 8,25 DEG C of temperature, time are 1 hour, during which observe water swelling situation.
Wherein, penetrating is mixed with pH adjusting agent in step 2 and the compound of water adjusts pH value to 7.
Wherein, in step 3, culture bottle volume is 1100mL, and Zai Pei bottle dress mixtures b weight is 750g, Zai Pei bottle tables
5 holes are made a call in face.
Further, the aperture of punching be 13.5-15mm in culture medium, each sky at intervals of 8-10mm, hole and bottle wall
Distance be 8-10mm.
Comparative example 1
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
2 parts of pH adjusting agent,
6 parts of water.
Wherein, the particle diameter of corncob is 3-6mm；Rice bran, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
Cotton seed hulls, sorghum husk and millet shell are without fermentation process.
Step 1：The component A raw material is weighed in proportion and adds water, is uniformly mixed, control time, is obtained culture medium and fill out
Charge mixture a；
Step 2：One side stir culture base inserts mixture a, adds B component raw material according to the above ratio on one side, and stirring is equal
After even, the stirring of another side, while spray into the pH adjusting agent compound, the water content for control pH adjusting agent compound is 68%,
PH value is 7.5, obtains mixture b.
Step 3：Mixture b is fitted into culture bottle, punching, gland, wherein entrucking sterilizing, sterilising conditions in culture medium
For：121 DEG C of temperature, pressure 0.11MPa, time 90min, are then cooled to less than 25 DEG C, obtain being used for true pleurotus cornucopiae and white beech mushroom
Culture medium.
Time used in preparation method whole process is 7h.
Wherein, penetrating is mixed with pH adjusting agent in step 2 and the compound of water adjusts pH value to 7.
Wherein, in step 3, culture bottle volume is 1100mL, and Zai Pei bottle dress mixtures b weight is 750g, Zai Pei bottle tables
5 holes are made a call in face.
Comparative example 2
A kind of culture medium for true pleurotus cornucopiae and white beech mushroom, include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
2 parts of pH adjusting agent,
6 parts of water.
Wherein, the particle diameter of corncob is 3-6mm；Rice bran, jowar shell, the particle diameter of millet shell and cotton seed hulls are 1-3mm.
Cotton seed hulls, sorghum husk and millet shell are gained after cotton seed hulls, sorghum husk and the fermented processing of millet shell；Fermentation process
Method is：Take cotton seed hulls, sorghum husk and millet shell to be positioned in fermentation vat, be well mixed, spray into water and continue to stir, control
Initial water content is 60-70%, pH 6.5-8, and fermentation process is carried out in 55 DEG C, fermentation time 3 days, during which, is surveyed within every 24 hours
Its water content and pH, and sprayed water according to actual water content and pH or drying and processing, its water content is maintained 60-70%,
Regulation pH is 6.5-8；After the completion of fermentation, by fermentation compound drying.
The preparation method of true pleurotus cornucopiae and white beech mushroom medium, comprises the following steps：
Step 1：Take corncob, wood chip, cotton seed hulls, sorghum husk, millet shell, soybean skin, wheat skin, oyster shell whiting, rice bran, dregs of beans
Powder, corn flour and lime are well mixed, spray into water, and control compound water content be 67%；The compound regulation pH of gained
To 6.2.
Step 2：The mixture obtained in step 1 is fitted into culture bottle, punching, gland, entrucking sterilizing in culture medium,
Wherein sterilising conditions are：121 DEG C of temperature, pressure 0.11MPa, time 90min, are then cooled to less than 25 DEG C, obtain being used for very
The culture medium of pleurotus cornucopiae and white beech mushroom.
Time used in preparation method whole process is 6.5h.
Wherein, in step 2, culture bottle volume is 1100mL, and Zai Pei bottle dress mixtures b weight is 750g, Zai Pei bottle tables
5 holes are made a call in face.
Embodiment 6
The cultivation of true pleurotus cornucopiae, comprises the following steps：
(1) culture medium in true pleurotus cornucopiae and white beech mushroom is taken, to the true pleurotus cornucopiae strain of the inoculation of medium；
(2) the growing environment temperature for controlling strain is 25 DEG C, the growing environment relative humidity of strain is 78%, the life of strain
CO in long environment2Concentration 2100-2500ppm, keep half-light culture, promote strain mycelial growth；
(3) growing state of mycelia is observed, after mycelia covers with bottle and maturation, carries out mycelium stimulation, fruiting；After fruiting, control
The temperature of the growing environment of mushroom is 16 DEG C, relative humidity control is 88%, CO2Concentration 3800ppm, intensity of illumination be
1100lux；
(4) when mushroom grows into set size, harvesting, true pleurotus cornucopiae is obtained.
Wherein, take the process of culture medium to be carried out in dust proof workshop in (1), be seeded in hundred grades of laminar flow environments and carry out；(3)
Middle humid control uses the cold artificial light sources of LED using the advanced stable fluid humidification process of dry fog two, illumination, and light source is 20% blue light
With the combination light of 80% white light, in (3), the opening after fruiting in culture bottle is arranged with horn-like cutting ferrule.
Embodiment 7
Difference with embodiment 6 is that in step (1) inoculation white jade mushroom strains, other conditions are same as Example 6.
Embodiment 8
The cultivation of true pleurotus cornucopiae, comprises the following steps：
(1) culture medium in true pleurotus cornucopiae and white beech mushroom is taken, to the true pleurotus cornucopiae strain of the inoculation of medium；
(2) the growing environment temperature for controlling strain is 20 DEG C, the growing environment relative humidity of strain is 75%, the life of strain
CO in long environment2Concentration 4000ppm, keep half-light culture, promote strain mycelial growth；
(3) growing state of mycelia is observed, after mycelia covers with bottle and maturation, carries out mycelium stimulation, fruiting；After fruiting, control
The temperature of the growing environment of mushroom is 16 DEG C, relative humidity control is 88%, CO2Concentration 4000ppm, intensity of illumination be
500lux；
(4) when mushroom grows into set size, harvesting, true pleurotus cornucopiae is obtained.
Wherein, take the process of culture medium to be carried out in dust proof workshop in (1), be seeded in hundred grades of laminar flow environments and carry out；(3)
Middle humid control uses the cold artificial light sources of LED using the advanced stable fluid humidification process of dry fog two, illumination, and light source is 20% blue light
With the combination light of 80% white light, in (3), the opening after fruiting in culture bottle is arranged with horn-like cutting ferrule.
Embodiment 9
Difference with embodiment 8 is that in step (1) inoculation white jade mushroom strains, other conditions are same as Example 8.
Embodiment 10
The cultivation of true pleurotus cornucopiae, comprises the following steps：
(1) culture medium in true pleurotus cornucopiae and white beech mushroom is taken, to the true pleurotus cornucopiae strain of the inoculation of medium；
(2) the growing environment temperature for controlling strain is 25 DEG C, the growing environment relative humidity of strain is 80%, the life of strain
CO in long environment2Concentration 2500-2700ppm, keep half-light culture, promote strain mycelial growth；
(3) growing state of mycelia is observed, after mycelia covers with bottle and maturation, carries out mycelium stimulation, fruiting；After fruiting, control
The temperature of the growing environment of mushroom is 18 DEG C, relative humidity control is 90%, CO2Concentration 4000ppm, intensity of illumination be
1500lux；
(4) when mushroom grows into set size, harvesting, true pleurotus cornucopiae is obtained.
Wherein, take the process of culture medium to be carried out in dust proof workshop in (1), be seeded in hundred grades of laminar flow environments and carry out；(3)
Middle humid control uses the cold artificial light sources of LED using the advanced stable fluid humidification process of dry fog two, illumination, and light source is 20% blue light
With the combination light of 80% white light, in (3), the opening after fruiting in culture bottle is arranged with horn-like cutting ferrule.
Embodiment 11
Difference with embodiment 10 is that in step (1) inoculation white jade mushroom strains, other conditions are same as in Example 10.
Experiment in cultivation example
The same terms that Example 1-5, comparative example 1 and culture medium made from comparative example 2 are pressed in embodiment 6 respectively are carried out
Inoculation and cultivation, are compared, as a result as shown in table 1 to the culture effect of true pleurotus cornucopiae.In true pleurotus cornucopiae fructification, the survey of crude protein
Determine method to survey for protein by GB 50095-2010 (measure of national food safety standard Protein in Food), detecting instrument
Determine instrument；The assay method of soluble saccharide is determined by GB/T6194-1986.Biological efficiency (%)=fructification fresh goods yield
(g)/culture medium dry weight (g) × 100%.
The true pleurotus cornucopiae of the different culture media of table 1 cultivation：
The same terms that Example 1-5, comparative example 1 and culture medium made from comparative example 2 are pressed in embodiment 7 respectively are carried out
Inoculation and cultivation, are compared, as a result as shown in table 2 to the culture effect of white beech mushroom.In white jade massee fruiting bodies, the survey of crude protein
Determine method to survey for protein by GB 50095-2010 (measure of national food safety standard Protein in Food), detecting instrument
Determine instrument；The assay method of soluble saccharide is determined by GB/T6194-1986.Biological efficiency (%)=fructification fresh goods yield
(g)/culture medium dry weight (g) × 100%.
The white beech mushroom of the different culture media of table 2 cultivation
The growth that culture medium provided by the present invention is advantageous to white beech mushroom, biology effect are can be seen that from the data in table
Rate is high, has higher nutritive value height, crude protein and Soluble carbohydrate content are high.
Above-mentioned detailed description is illustrating for one of them possible embodiments of the present invention, and the embodiment is simultaneously not used to
The scope of the claims of the present invention is limited, all equivalence enforcements or change without departing from carried out by the present invention, is intended to be limited solely by the technology of the present invention
In the range of scheme.
Claims (10)
1. a kind of culture medium for true pleurotus cornucopiae and white beech mushroom, it is characterised in that include the component of following parts by weight：
Component A:Culture medium inserts
B component：Culture medium fuel
Component C：PH adjusting agent compound
PH adjusting agent 0.1-2 parts,
Water 0.3-8 parts,
The culture medium can be not only used for the strain that the strain of Liquid Culture can be used for solid culture again.
A kind of 2. culture medium for true pleurotus cornucopiae and white beech mushroom according to claim 1, it is characterised in that the pH regulations
Agent is at least one of lime, oyster shell whiting, precipitated calcium carbonate, lightweight calcium sulfate.
3. the preparation method of the culture medium of true pleurotus cornucopiae and white beech mushroom as described in claim any one of 1-2, it is characterised in that bag
Include following steps：
Step 1：The component A raw material is weighed in proportion, is added the pre- wet fermentation expansion process of water, is drained away the water, be uniformly mixed,
Control time, obtain culture medium inserts mixture a；
Step 2：One side stir culture base inserts mixture a, add B component raw material according to the above ratio on one side, after stirring,
Another side stirring, while spraying into the pH adjusting agent compound, the water content for controlling pH adjusting agent compound is 60-70%, pH
It is worth for 6.0-8.0, obtains mixture b；
Step 3：Mixture b is fitted into culture bottle, punching, gland, entrucking sterilizing in culture medium, wherein sterilising conditions are：
116-125 DEG C of temperature, pressure 0.080-0.135MPa, time 80-90min, are then cooled to less than 25 DEG C, obtain being used for a true Ji
Mushroom and the culture medium of white beech mushroom.
4. the preparation method of true pleurotus cornucopiae according to claim 3 and white beech mushroom medium, it is characterised in that in step 1, institute
Stating pre- wet fermentation expansion process includes weighing component A raw material in proportion, addition suitable quantity of water, control initial water content 65-75%,
PH value is 6.0-8.0,20-25 DEG C of temperature, time are 1-2 hours, during which observes water swelling situation.
5. the preparation method of true pleurotus cornucopiae according to claim 3 and white beech mushroom medium, it is characterised in that the step 2
In, the compound that pH adjusting agent and water are mixed with by spraying into adjusts pH value to 7.
6. the preparation method of the culture medium of true pleurotus cornucopiae according to claim 3 and white beech mushroom, it is characterised in that：In step 3,
Described culture bottle volume is 850mL or 1100mL, and wherein 850mL Zai Pei bottle dress mixtures b weight is 500-600g, is planted
Pei bottle beat 1-3 hole in surface；1100mL Zai Pei bottle dress mixtures b weight is 650-750g, and Zai Pei bottle beat on surface 1-5
Hole.
7. the preparation method of true pleurotus cornucopiae according to claim 3 and white beech mushroom medium, it is characterised in that the step 3
In, sterilising conditions are that culture medium sterilizes 80-85min at 121 DEG C of temperature, pressure 0.11MPa.
8. a kind of application for true pleurotus cornucopiae and the culture medium of white beech mushroom, it is characterised in that application is such as any institutes of claim 1-7
The true pleurotus cornucopiae of the culture medium cultivation for being used for true pleurotus cornucopiae and white beech mushroom or white beech mushroom stated, comprise the following steps：
(1) culture medium in true pleurotus cornucopiae and white beech mushroom is taken, to the true pleurotus cornucopiae strain of the inoculation of medium or white jade mushroom strains；
(2) the growing environment temperature for controlling strain is 20-25 DEG C, the growing environment relative humidity of strain is 75-80%, strain
CO in growing environment2Concentration≤4000ppm, keep half-light culture, promote strain mycelial growth；
(3) growing state of mycelia is observed, after mycelia covers with bottle and maturation, carries out mycelium stimulation, fruiting；After fruiting, mushroom is controlled
The temperature of growing environment is 13-18 DEG C, relative humidity control is 85-100%, CO2Concentration≤4000ppm, intensity of illumination be
500-1500lux；
(4) when mushroom grows into set size, harvesting, true pleurotus cornucopiae or white beech mushroom are obtained.
A kind of 9. application for true pleurotus cornucopiae and the culture medium of white beech mushroom according to claim 8, it is characterised in that：(1)
In take the process of culture medium to be carried out in dust proof workshop, be seeded in hundred grades of laminar flow environments and carry out；(3) humid control uses first in
Enter the stable fluid humidification process of dry fog two, illumination uses the cold artificial light sources of LED, and light source is the combination of 20% blue light and 80% white light
Light.
10. a kind of application for true pleurotus cornucopiae and the culture medium of white beech mushroom according to claim 8, it is characterised in that (3)
In, the opening after fruiting in culture bottle is arranged with horn-like cutting ferrule.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111480514A (en) * | 2020-05-08 | 2020-08-04 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of hypsizigus marmoreus |
| CN112243795A (en) * | 2020-10-19 | 2021-01-22 | 江苏华绿生物科技股份有限公司 | Millet shell culture medium and application thereof in industrial cultivation of white flammulina velutipes |
| CN115093976A (en) * | 2022-06-27 | 2022-09-23 | 山东省农业科学院 | Hypsizygus marmoreus ZHSjg-byg and cultivation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0919219A (en) * | 1995-07-05 | 1997-01-21 | New Oji Paper Co Ltd | Indoor cultivation of lyophyllum decastes sing. |
| CN104909896A (en) * | 2015-06-05 | 2015-09-16 | 电白中茂生物科技有限公司 | Solid medium as well as preparation method and application thereof |
-
2017
- 2017-11-27 CN CN201711207921.5A patent/CN107867934A/en not_active Withdrawn
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0919219A (en) * | 1995-07-05 | 1997-01-21 | New Oji Paper Co Ltd | Indoor cultivation of lyophyllum decastes sing. |
| CN104909896A (en) * | 2015-06-05 | 2015-09-16 | 电白中茂生物科技有限公司 | Solid medium as well as preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 余养健等: "《食用菌绿色栽培10项关键技术》", 30 September 2013, 金盾出版社 * |
| 肖自添等: "《草菇优质生产技术》", 30 June 2017, 中国科学技术出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111480514A (en) * | 2020-05-08 | 2020-08-04 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of hypsizigus marmoreus |
| CN112243795A (en) * | 2020-10-19 | 2021-01-22 | 江苏华绿生物科技股份有限公司 | Millet shell culture medium and application thereof in industrial cultivation of white flammulina velutipes |
| CN115093976A (en) * | 2022-06-27 | 2022-09-23 | 山东省农业科学院 | Hypsizygus marmoreus ZHSjg-byg and cultivation method thereof |
| CN115093976B (en) * | 2022-06-27 | 2023-05-09 | 山东省农业科学院 | White beech mushroom ZHSjg-byg and cultivation method thereof |
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