CN101433275B - Method for preparing Rhodotorula benthica fermentation feed - Google Patents

Method for preparing Rhodotorula benthica fermentation feed Download PDF

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Publication number
CN101433275B
CN101433275B CN2008102387102A CN200810238710A CN101433275B CN 101433275 B CN101433275 B CN 101433275B CN 2008102387102 A CN2008102387102 A CN 2008102387102A CN 200810238710 A CN200810238710 A CN 200810238710A CN 101433275 B CN101433275 B CN 101433275B
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fermentation
solid fermentation
feed
rhodotorula
preparation
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CN101433275A (en
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牟海津
王鹏
葛蕾蕾
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Ocean University of China
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Ocean University of China
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention relates to a method for preparing ocean rhodotorula fermentation feed. The method comprises the preparation of solid fermentation base stock of soybean meal, the mixing of a nutrition replenishing agent and the preparation of a solid fermentation medium. The feed is characterized in that ocean rhodotorula and a lactobacillus strain are inoculated in the solid fermentation medium andare subjected to solid fermentation to obtain the ocean rhodotorula fermentation feed. The method has the advantages: 1. macromolecular protein in the soybean meal is decomposed into small molecular peptide which improves the digestive absorption rate of the protein and further balances the composition of amino acid through microbial transformation; 2. the fermentation feed is replenished with astaxanthin and other biological active substances introduced by the metabolism of rhodotorula glutinis, thereby improving the nutritive value of the feed; 3. lactobacillus in the fermentation feed has positive function on improving a microecological flora of intestinal tracts of bred animal; and 4. the method has low fermentation cost, low fermentation condition and equipment requirement, simple operation of process flow and small investment and is suitable for small-scale production of breeding households and feed processing factories.

Description

A kind of preparation method of Rhodotorula benthica fermentation feed
Technical field
The present invention relates to a kind of feed, particularly relate to a kind of preparation method of Rhodotorula benthica fermentation feed.
Background technology
In recent years, along with the development of China's aquaculture, the demand of high protein feed is increased year by year.The crude protein content of dregs of beans is up to 43%～48%; And form by amino acid than balance; Especially contain other plant feed and be prone to scarce lysine; And have splendid animal absorptivity, thereby receive Feed Enterprise and raiser's favor, become the maximum indispensable protein feed of ratio in the forage compounding.Yet, also there is the multiple ANFs that comprises trypsin inhibitor in the dregs of beans, greatly influenced the feeding value of dregs of beans.Be used further to the bait processing of cultivated animals after adopting biological fermentation process that dregs of beans is handled, can reach the purpose of under the situation that does not influence the dregs of beans nutritional labeling, removing ANFs, also have positive effect for the nutritive value that improves dregs of beans simultaneously.Existing at present about utilizing independent fermentation such as saccharomycete, lactic acid bacteria and bacillus subtilis or mixed culture fermentation to remove the report of ANFs in the dregs of beans; The used yeast bacterium comprises shadow yeast, candida tropicalis, Candida arborea etc., but does not see the report that utilizes the ocean rhodotorula fermented bean dregs to produce feed as yet.
Summary of the invention
The preparation method who the purpose of this invention is to provide a kind of Rhodotorula benthica fermentation feed; It can effectively remove the ANFs in the bean-dregs feed; Improve unrighted acid, small protein and content of amino acids, improve digestion and the absorption efficiency of cultivated animals, effectively replenish physiological activators such as astaxanthin dregs of beans; And help to improve cultivated animals intestinal microecology fauna, thereby improve the nutritive value of bean-dregs feed.
A kind of preparation method of Rhodotorula benthica fermentation feed; Comprise preparation dregs of beans solid fermentation base-material; Sneak into nutritious supplementary pharmaceutical; Process the solid fermentation culture medium, it is characterized in that behind solid fermentation, obtaining Rhodotorula benthica fermentation feed to solid fermentation inoculation of medium ocean rhodotorula and lactic acid bacteria culturers.
Advantage of the present invention is: 1, macro-molecular protein is decomposed into micromolecular peptide class in the dregs of beans, has improved the digestibility of protein, and through the further balance of microbial conversion amino acid whose composition; 2, replenish the physiological activators of introducing owing to the rhodotorula glutinis metabolism such as astaxanthin in the fermented feed, improved the nutritive value of feed; 3, the lactic acid bacteria in the fermented feed has positive effect for the little ecosystem that improves the cultivated animals enteron aisle; 4, cheap, fermentation condition of fermentation costs and equipment requirements are low, and technological process is easy and simple to handle, invest for a short time, are fit to culture the small-scale production of producer and feed-processing plant.
The specific embodiment
Ocean rhodotorula described in the present invention is rhodotorula glutinis or rhodothece rubra, and described lactic acid bacteria is Lactobacillus plantarum, picogram lactobacillus or Pediococcus acidilactici; Described preparation dregs of beans solid fermentation base-material: after dregs of beans, peanut meal, wheat bran pulverized, mix according to the part by weight of 12-20: 0-6: 0-2; Described nutritious supplementary pharmaceutical is by yeast extract, KH 2PO 4, NaCl, (NH 4) 2SO 4, sucrose and water forms, thousand fens concentration of their weight respectively is 2-5 ‰, 0.1-0.4 ‰, 0.5-1.5 ‰, 1.5-4 ‰, 20-40 ‰, all the other are water, pH is 5.5-6.0, the available HCl or the NaOH aqueous solution are regulated; Described solid fermentation culture medium is made up of solid fermentation base-material and nutritious supplementary pharmaceutical, and both part by weight scopes are 1: 1.1-1.4, during preparation, they are mixed, and boiling is more than 5 minutes; Described solid fermentation: the ocean rhodotorula and the lactobacillus solution that will be cultured to logarithmic phase; Be that the ratio of 4-10% and 2-5% is seeded in the solid fermentation culture medium according to concentration expressed in percentage by weight respectively; Thickness by 2-12cm after stirring is laid in the pallet; Tight with double-deck polypropylene film capping, in 28-40 ℃ of solid fermentation 24-72h, and guarantee that ambient humidity is more than 80%.
After fermentation was accomplished, fermented bean dregs can be pulverized behind low temperature drying below 65 ℃; For the product of instant use, needn't dry processing, can guarantee the physiologically active of trophic factors such as astaxanthin in the fermented bean dregs so better, also can reduce the part beneficial microorganism loss that causes because of oven dry.
Embodiment one
After dregs of beans, peanut meal and wheat bran pulverizing, the three is mixed according to 17: 2: 1 part by weight, process 1000g dregs of beans solid fermentation base-material; In addition with yeast extract 5g, KH 2PO 40.2g, NaCl 1g, (NH 4) 2SO 43.5g, sucrose 40g and water mixes, and processes the 1000g nutritious supplementary pharmaceutical, using the HCl aqueous solution adjust pH of 1M is 6.0; Above-mentioned solid fermentation base-material is sneaked into nutritious supplementary pharmaceutical, and both mix according to 1: 1.2 part by weight, process the solid fermentation culture medium, in the polyethylene plastic bag of packing into, tighten sack, in 100 ℃ of boilings 8 minutes, and cooling.
During inoculation; Prepare the rhodotorula glutinis seed liquor earlier: primary inclined plane is cultivated and is adopted potato culture; After 24 hours, inoculate secondary marine yeast fluid nutrient medium in 32 ℃ of cultivations, this culture medium is formed: glucose 2%, peptone 1%, yeast extract 0.5%, sodium chloride 2%; The pH value is 5.5, can be to the logarithmic phase of growing in 32 ℃ of shaken cultivation 24-36 hours.Prepare the Lactobacillus plantarum seed liquor again: primary inclined plane is cultivated and is adopted improvement tomato juice culture medium, after 48 hours, inoculates secondary MRS fluid nutrient medium in 32 ℃ of cultivations, leaves standstill in 32 ℃ and cultivates 48-72 hour to the logarithmic phase of growing.The rhodotorula glutinis that is cultured to logarithmic phase and Lactobacillus plantarum seed liquor are seeded in the above-mentioned solid fermentation culture medium according to 6% and 2% ratio respectively, after stirring, are laid in the pallet by the thickness of 6cm; Tight with double-deck polypropylene film capping; In 32 ℃ of solid fermentation 48h, ambient humidity is more than 80%, but the running water that sprays scalding every day to top surface is to improve humidity; Turn 3-4 time with aseptic shovel every day, and culture is mixed.
During experiment, it is the fermentation frame of 1.5m that fermenting cellar is equipped with total height, can put 8 layered materials, the about 20cm of every layer height, every layer of every m 2But blowing 7.5kg (with dry weight basis), every like this m 2Floor space can in two days, to gather in the crops dry weight be this fermented bean dregs of 60kg.
After fermentation was accomplished, through the blood counting chamber counting, rhodotorula glutinis thalline quantity can reach 4,200,000,000/gram dry weight, and the pH value is about 6.0, and the fermentate smell is acid slightly, and have stronger pure fragrance, does not have unhappy smell, and color and luster obviously shoals pinkiness before the fermentation.
In the present embodiment, described rhodotorula glutinis can be used the rhodothece rubra in the ocean rhodotorula instead, and described Lactobacillus plantarum can be used Pediococcus acidilactici, the picogram lactobacillus in the lactic acid bacteria instead, all can obtain similar effects.
Embodiment two
Dregs of beans and peanut meal are pulverized, the two part by weight according to 19: 1 is mixed, process 1000g dregs of beans solid fermentation base-material; In addition with yeast extract 5g, KH 2PO 40.1g, NaCl 1.5g, (NH 4) 2SO 41.5g, sucrose 30g and water mixes, and processes the 1000g nutritious supplementary pharmaceutical, using the HCl aqueous solution adjust pH of 1M is 6.0; Above-mentioned solid fermentation base-material is sneaked into nutritious supplementary pharmaceutical, and both mix according to 1: 1.1 part by weight, process dregs of beans solid fermentation culture medium, in 100 ℃ of boilings 8 minutes, and cooling.
During inoculation; The rhodothece rubra that is cultured to logarithmic phase and Pediococcus acidilactici seed liquor are seeded in the above-mentioned dregs of beans solid fermentation culture medium according to 10% and 4% ratio respectively; After stirring; Thickness by 4cm is laid in the pallet, and is tight with double-deck polypropylene film capping, in 32 ℃ of solid fermentation 48h.After fermentation was accomplished, through the blood counting chamber counting, rhodothece rubra thalline quantity was 2,600,000,000/gram dry weight, and the pH value is about 6.0, and the fermentate smell is acid slightly, and have stronger pure fragrance, does not have unhappy smell, and color and luster shows slightly bright-coloured before the fermentation.

Claims (2)

1. the preparation method of a Rhodotorula benthica fermentation feed; Comprise preparation dregs of beans solid fermentation base-material; Sneak into nutritious supplementary pharmaceutical; Process the solid fermentation culture medium, it is characterized in that behind solid fermentation, obtaining Rhodotorula benthica fermentation feed to solid fermentation inoculation of medium ocean rhodotorula and lactic acid bacteria culturers; Described preparation dregs of beans solid fermentation base-material: after dregs of beans, peanut meal, wheat bran pulverized, mix according to the part by weight of 12-20: 0-6: 0-2; Described nutritious supplementary pharmaceutical is by yeast extract, KH 2PO 4, NaCl, (NH 4) 2SO 4, sucrose and water forms, thousand fens concentration of their weight respectively is 2-5 ‰, 0.1-0.4 ‰, 0.5-1.5 ‰, 1.5-4 ‰, 20-40 ‰, all the other are water, pH is 5.5-6.0; Described solid fermentation is: the ocean rhodotorula and the lactobacillus solution that will be cultured to logarithmic phase; Be that the ratio of 4-10% and 2-5% is seeded in the solid fermentation culture medium according to concentration expressed in percentage by weight respectively; Thickness by 2-12cm after stirring is laid in the pallet; Tight with double-deck polypropylene film capping, in 28-40 ℃ of solid fermentation 24-72h, ambient humidity is more than 80%.
2. the preparation method of Rhodotorula benthica fermentation feed according to claim 1 is characterized in that described solid fermentation culture medium is made up of solid fermentation base-material and nutritious supplementary pharmaceutical, and both part by weight scopes are 1: 1.1-1.4.
CN2008102387102A 2008-12-16 2008-12-16 Method for preparing Rhodotorula benthica fermentation feed Expired - Fee Related CN101433275B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103416582A (en) * 2013-08-02 2013-12-04 辽宁爱普罗斯饲料有限公司 Microorganism pellet feed and manufacturing method thereof

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CN101914460B (en) * 2010-07-13 2013-09-11 广州市博善生物饲料有限公司 Method for fermenting phodotorula benthica culture
CN101912032B (en) * 2010-08-12 2012-02-29 黑龙江大荒春酒业有限公司 Method for preparing rhodotorula glutinis feed
CN102329741B (en) * 2011-09-27 2013-02-06 浙江省农业科学院 Method for producing fodder rhodotorula through mixed solid fermentation
CN102894237A (en) * 2012-11-08 2013-01-30 黑龙江省轻工科学研究院 Method for preparing feed additive for red-feather serinus canaria
CN103431199A (en) * 2013-09-13 2013-12-11 黑龙江省轻工科学研究院 Production method of pseudosciaena crocea feed additive
CN103602602A (en) * 2013-12-01 2014-02-26 领绿生物镇江有限公司 Oceanic red yeast solid-fermentation method
CN103845370B (en) * 2014-01-03 2017-07-11 青岛东海药业有限公司 A kind of rhodotorula glutinis bacteria composition for being applied to sea cucumber, abalone culture
CN104830703B (en) * 2015-05-13 2018-09-11 威海利达生物科技有限公司 One plant of rhodotorula mucilaginosa and its application in the red astaxanthin in fermenting and producing sea
CN105861615A (en) * 2016-04-22 2016-08-17 闫大凤 Production method of amino acid bioactive peptide for aquatic products
CN107319129A (en) * 2017-05-19 2017-11-07 北京伟农生物科技有限公司 A kind of preparation of fermented bean dregs rich in astaxanthin and feed
CN108041339A (en) * 2017-11-15 2018-05-18 兰溪市酉泽饲料技术服务有限公司 Add the prawn children phase feed of Sage extract
CN108157667A (en) * 2017-11-15 2018-06-15 兰溪市酉泽饲料技术服务有限公司 Add the preparation method of the shallow sea fish meal of ocean rhodotorula extract
CN108185182A (en) * 2018-01-06 2018-06-22 大连三净集团有限公司 A kind of preparation method of TMPF biofermentation mixed feeds that can improve meat quality
CN108185190A (en) * 2018-03-23 2018-06-22 哈尔滨伟平科技开发有限公司 A kind of preparation method of pig feed additive
CN109548984A (en) * 2018-12-19 2019-04-02 江南大学 A method of oil crops are processed using rhodotorula

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1117803A (en) * 1994-08-29 1996-03-06 黄殿鹏 Novel feed (probiotics) and production process thereof
CN1415740A (en) * 2002-10-15 2003-05-07 湖北安琪酵母股份有限公司 Drying production technique by bran adsorption for drying oceanic rhodotorula
CN1435112A (en) * 2002-01-30 2003-08-13 康建国 Method for preparing biological feed from waste mycelium residue of beta-lactam antibioties
CN1602724A (en) * 2004-09-14 2005-04-06 王金华 Fowl egg natural pigment feed production method

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1117803A (en) * 1994-08-29 1996-03-06 黄殿鹏 Novel feed (probiotics) and production process thereof
CN1435112A (en) * 2002-01-30 2003-08-13 康建国 Method for preparing biological feed from waste mycelium residue of beta-lactam antibioties
CN1415740A (en) * 2002-10-15 2003-05-07 湖北安琪酵母股份有限公司 Drying production technique by bran adsorption for drying oceanic rhodotorula
CN1602724A (en) * 2004-09-14 2005-04-06 王金华 Fowl egg natural pigment feed production method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
周与良等.中国海红酵母属(Rhodotoru la Harr ison) 的种类.《南开大学学报（自然科学）》.1999,第32卷(第4期),115-116. *
石勇等.红酵母产类胡萝卜素固态发酵工艺条件的研究.《生物技术》.2006,第16卷(第4期),65-68. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103416582A (en) * 2013-08-02 2013-12-04 辽宁爱普罗斯饲料有限公司 Microorganism pellet feed and manufacturing method thereof

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