CN109463273A - A kind of disease-resistant High-Efficient Cotton breeding method of Early maturity - Google Patents
A kind of disease-resistant High-Efficient Cotton breeding method of Early maturity Download PDFInfo
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 171
- 201000010099 disease Diseases 0.000 title claims abstract description 87
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 87
- 238000009395 breeding Methods 0.000 title claims abstract description 61
- 230000001488 breeding effect Effects 0.000 claims abstract description 43
- 208000035240 Disease Resistance Diseases 0.000 claims abstract description 38
- 238000012216 screening Methods 0.000 claims abstract description 26
- 238000009396 hybridization Methods 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 6
- 241000219146 Gossypium Species 0.000 claims description 168
- 241000196324 Embryophyta Species 0.000 claims description 57
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims description 23
- 239000000835 fiber Substances 0.000 claims description 8
- 238000003306 harvesting Methods 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 4
- 239000004575 stone Substances 0.000 claims description 4
- 238000012360 testing method Methods 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 8
- 235000013399 edible fruits Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 235000009429 Gossypium barbadense Nutrition 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000018322 upland cotton Nutrition 0.000 description 2
- 238000013475 authorization Methods 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 230000010152 pollination Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000012372 quality testing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
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Abstract
The invention belongs to cotton breeding technical fields, and in particular to a kind of disease-resistant High-Efficient Cotton breeding method of Early maturity.Screening obtains the backbone parent of cotton Early maturity breeding for disease resistance, hybridizes the acquisition of bell, the acquisition of the disease-resistant high yield high-quality cotton new lines of Early maturity.Parent is planted in artificial withered and yellow wither and mixes sick garden in breeding method of the present invention, selects single plant preparing hybrid disease-resistant, that growth potential is strong to combine in full-bloom stage, hybridization bell obtained just has good disease resistance.F in breeding method of the present invention1、F2、F4、F6In four generations, mixed sick garden plantation, stress screening in artificial withered and yellow wither, and the new varieties for further ensuring institute's breeding have stronger disease resistance；F3、F5It is planted for winter in Hainan, so that the breeding time limit is shortened 2 years, accelerate breeding process.
Description
Technical field
The invention belongs to cotton breeding technical fields, and in particular to a kind of disease-resistant High-Efficient Cotton breeding method of Early maturity.
Background technique
Cotton is the major economic crops in China.The cotton per unit area yield and total yield in China rank first in the world.In recent years China
Cotton area is at 50,000,000 mu or more.Xinjiang light-heat water and soil resource is abundant, and suitable cotton region domain is wide, in recent years cotton in Xinjiang growing surface
At 30,000,000 mu or more, per unit area yield, total yield occupy the whole nation and plant first of cotton province (autonomous region) product.Xinjiang is also that China is most sent out
The high quality cotton main producing region of potentiality is opened up, it is the important plant cotton region in Xinjiang that wherein there are about 8,000,000 mu or so of cotton fields in North SinKiang precocity plant cotton region
One of, and the high-quality advantageous ecotope of upland cotton is planted, the high-quality raw cotton of ecotope production accounts for whole nation outlet cotton
80% or more, it is the most important outlet cotton base in China and Xinjiang.As the continuous cropping time limit in cotton field extends, cotton spoting verticillium wilt
Sprawling rapidly, it has also become cotton continues the major obstacle of high yield, stable yields.The cotton field of complete 80% or more boundary is by blight at present
Harm.Blight has been one of the principal element for restricting Development of Cotton Production in Xinjiang Region.Xinjiang needs precocious, disease-resistant, high yield
(40% or more ginning outturn, medium blade size, plant type are moderate), good new cotton variety.It is good according to distant cotton variety prematureness,
The prominent feature of disease resistance, in the breeding times such as North SinKiang Usu, Kuitun, Shawan, Shihezi, Manas 125 days or so regions,
Experiment and demonstration and the high-quality distant cotton variety of the precocious disease-resistant high yield of popularization have broad prospects.
Since the cotton area of Inner Mongol is gradually expanded, and the ecotope in Liaoning and Inner Mongol are close, and distant cotton variety is than convenient
It is planted in Inner Mongol area, so distant cotton is covered with wide application prospect inside, and distant cotton No. 15 is more in Inner Mongol plantation
Year, it has outstanding performance, the welcome by local cotton grower.For 2013 and 2015 distant cottons No. 23 in Inner Mongol experiment and demonstration, yielding ability is good
It is good, therefore the high-quality distant cotton new varieties of Early maturity disease-resistant high yield are cultivated, it is significant to promoting Xinjiang, Inner Mongol Cotton Production to develop.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of disease-resistant High-Efficient Cotton breeding method of Early maturity.
To achieve the above object, the invention adopts a technical scheme as:
A kind of disease-resistant High-Efficient Cotton breeding method of Early maturity:
1) screening obtains the backbone parent of cotton Early maturity breeding for disease resistance: in northeast, cotton planting district utilizes plantation for many years
Cotton resource (i.e. distant cotton), pass through the standards of grading and appraisement system of the high-quality distant cotton of the disease-resistant high yield of Early maturity, select Early maturity
The disease-resistant good cotton variety of high yield is as backbone parent；
2) hybridize the acquisition of bell: mixing that the backbone parent is planted in sick garden and bell is more, bell is big, high clothing in artificial withered and yellow wither
Point, it is good it is outer draw cotton resource, according to character principle of complementarity, in full-bloom stage, hybridization obtains F0Generation hybridization bell；
3) numerous (F in south the acquisition of the disease-resistant high yield high-quality cotton new lines of Early maturity: is passed through using pedigree method3Generation and F5Generation) north
Educate (F1Generation, F2Generation, F4Generation and F6Generation, and sick garden F is mixed in artificial withered and yellow wither1Generation, F2Generation, F4Generation and F6In 4 generations in generation, coerce sieve
Choosing), to obtain, the disease resistance Early maturity (breeding time≤128 day) stable compared with strong, character, disease-resistant (wilt disease refers to≤10, Huang
Disease of withering refers to≤20), high yield (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede length >=29mm, than by force >=30cN/tex, mark
Grand value=3.5-5.0) F6For New cotton line.
The step 2) by step 1) obtain backbone parent with draw cotton resource preparing hybrid outside and combine, hybridize acquisition F0
The hybridization bell of acquisition is then planted in artificial withered and yellow wither and mixes sick garden selfing acquisition F1 generation plant, to F by generation hybridization bell1For miscellaneous
Combination is handed over to be screened, harvest Early maturity (breeding time≤128 day), disease-resistant (wilt disease refers to that≤10, verticillium wilt refers to≤20), rich
The cross combination of (5 meter Hang Chang unginned cotton's weight >=0.9kg) is produced, for use.
Wherein, combination refers to that " backbone parent × source of bringing in funds outside " or " source × backbone parent of bringing in funds outside " i.e. backbone parent is made
Maternal or male parent cross combination.
By above-mentioned screened F1Sick garden plantation is mixed in artificial withered and yellow wither for cross combination, selfing obtains F2Generation separation is single
Strain, (wadding bell number >=15 are opened in single basal munure >=18 for screening Early maturity (breeding time≤128 day), disease-resistant (not falling ill), high yield
It is a), high-quality (suede length >=29mm) F2For single plant, for use；
Above-mentioned screening is obtained into F2It is planted in winter current year in Hainan for single plant, selfing obtains F3For plant, screen excellent
(neatly, open and wad a quilt with cotton) plant；
F will be obtained3Sick garden plantation is mixed in northern artificial withered and yellow wither again in spring for plant, selfing obtains F4For strain, sieve
Select Early maturity (breeding time≤128 day), disease-resistant (wilt disease refers to that≤10, verticillium wilt refers to≤20), high yield (5 meter Hang Chang seed cotton yields
>=0.9kg), high-quality (suede length >=29mm, than strong >=30cN/tex, mic value=3.5-5.0) strain；
The strain of above-mentioned screening is planted in winter in this year in Hainan, selfing obtains F5For strain；
By excellent (neatly, open and the wad a quilt with cotton) F of the numerous acquisition in above-mentioned south5For strain in spring to it is northern artificial it is withered and yellow wither mix it is sick
Garden plantation, selfing obtain Early maturity (breeding time≤128 day), disease-resistant (wilt disease refers to that≤10, verticillium wilt refers to≤20), high yield (5
Meter Hang Chang seed cotton yield >=0.9kg), high-quality (suede length >=29mm, than strong >=30cN/tex, mic value=3.5-5.0) character
Stable F6For strain, the i.e. disease-resistant high yield high-quality cotton new lines of Early maturity.
The standards of grading and appraisement system by the high-quality distant cotton of the disease-resistant high yield of Early maturity, select Early maturity disease-resistant rich
The backbone parent for producing high-quality cotton is distant cotton No. 23, distant cotton No. 27, distant cotton 31 or distant cotton 34；
The bell is more, bell is big, Gao Yifen, it is good it is outer draw cotton resource be stone is remote 321, letter is closed in Xinjiang, Ji Mian 169, in
Plant cotton No. 2, Xinluzao No.1, R001, HGBMHR-H, Xinjiang KW-37, P10-1, Shanxi 0316 or 14244612.
The kind technical indicator of the screening:
(1) Reproduction period index: Exceptional early mature cotton region≤128 day such as Liaoning；
(2) fiber quality traits: I type: suede length >=31mm, specific strength >=32cN/tex, mic value 3.7-4.2； Ⅱ
Type: suede length >=29mm, specific strength >=30cN/tex, mic value 3.5-5.0；III type: suede length >=27mm, specific strength >=28cN/
Tex, mic value 3.5-5.5；
(3) yield index: I type: pre-frost lint yield is than local main breed (the Liao Dynasty 23) volume increase >=0.0%；II type: frost
Preceding lint yield is than local main breed (the Liao Dynasty 23) volume increase >=3.0%；III type: pre-frost lint yield is than local main breed (the Liao Dynasty
23) increase production >=8.0%.Ginning outturn reaches 40% or more；Flower rate >=85% before frost；
(4) Resistance index: wilt disease refers to≤20, and verticillium wilt refers to≤35.
Compared with prior art, the invention has the following advantages that
1, Parent is planted in artificial withered and yellow wither and mixes sick garden in breeding method of the present invention, selects in full-bloom stage disease-resistant, raw
The strong single plant preparing hybrid combination of growing way, hybridization bell obtained just have good disease resistance.
2, F in breeding method of the present invention1、F2、F4、F6In four generations, mixed sick garden plantation in artificial withered and yellow wither, stress screening, into
One step ensure that the new varieties of institute's breeding have stronger disease resistance；F3、F5It is planted for winter in Hainan, shortens the breeding time limit
2 years, accelerate breeding process.
3, breeding method of the present invention is to be combined using local backbone parent with the cotton resource preparing hybrid that other places is introduced,
Filial generation acceptance rate (50%) improves 9.5% (being shown in Table 3 and table 4) than general Parent hybridization acceptance rate (40.5%), from
And substantially increase crossbreeding efficiency；Backbone parent either makees female parent simultaneously, still makees male parent, the cross combination of preparation
Offspring's acceptance rate is all higher, and has filtered out a collection of excellent strain (being shown in Table 5).
Specific embodiment
The present invention is described in detail combined with specific embodiments below, but should not be construed as limitation of the invention.It is following
The test method of actual conditions is not specified in embodiment, operates usually according to normal condition, due to not being related to inventive point, therefore not
Its step is described in detail.
Distant cotton No. 9 and distant cotton No. 27 are now stored in industrial crops research institute, Liaoning Province, at the same distant cotton No. 9 be recorded in it is " special early
No. 9, Cai Shenglin, He Renrui chief editors of ripe upland cotton new varieties the Liao Dynasty cotton, " Chinese agriculture yearbook " 1992 "；Distant cotton No. 27 is recorded in
" the Cotton such as the Early maturity disease-resistant high yield new cotton variety-of the Liao Dynasty cotton No. 27 Liu Yanzhen, Wang Zisheng, Cao Guiyan, 2013,40
(8): 39 " one texts.
" the disease-resistant High-Efficient Cotton breeding method of Early maturity " provided by the present invention is as follows:
1, the disease-resistant high yield high-quality cotton backbone parent of Early maturity is screened
In northeast, cotton planting district is excellent by the disease-resistant high yield of Early maturity using the cotton resource (i.e. distant cotton) planted for many years
The standards of grading (referring to table 2) and appraisement system (referring to table 1) of matter the Liao Dynasty cotton select the disease-resistant good cotton product of high yield of Early maturity
Kind is used as backbone parent；
The high-quality distant cotton backbone parent of the disease-resistant high yield of Early maturity for being suitable for the Exceptional early mature cotton region such as Liaoning, North SinKiang, Inner Mongol according to table 2
This standards of grading and table 1 are suitable for the high-quality distant cotton backbone parent of the disease-resistant high yield of the Early maturity of the Exceptional early mature cotton region such as Liaoning, North SinKiang, Inner Mongol
This appraisement system, filter out overall merit 90 divide more than backbone parent 4, be distant cotton No. 23, distant cotton No. 27, distant cotton respectively
31 and distant cotton 34.
Table 1 is suitable for the high-quality distant cotton backbone parent evaluation of the disease-resistant high yield of the Early maturity of the Exceptional early mature cotton region such as Liaoning, North SinKiang, Inner Mongol
System
Prematureness: in Liaoning cotton region breeding time=124-128 days
Table 2 is suitable for the high-quality distant cotton backbone parent scoring of the disease-resistant high yield of the Early maturity of the Exceptional early mature cotton region such as Liaoning, North SinKiang, Inner Mongol
Standard
2, preparing hybrid combines
Sick garden plantation above-mentioned " backbone parent " and the big (bell of bell more (single basal munure >=20), bell are mixed in artificial withered and yellow wither
Weight >=6.0g), Gao Yifen (ginning outturn >=40%), high-quality (suede length >=29mm, than strong >=30cN/tex, mic value=3.5-
5.0) it brings in funds source outside.According to character principle of complementarity, in full-bloom stage, selects " backbone parent " and cotton resource disease resistance is good (does not send out
Disease), the strong single plant of growth potential, preparing hybrid combination, by the way that the stamen of flower of female parent is stripped, with male parent flower flower of female parent column
It pollinates on head, to take acquisition hybridization bell in female parent pollination, and then obtains the good hybridization bell of disease resistance.
3, the disease-resistant high yield high-quality cotton new lines screening of offspring's Early maturity
Using pedigree method, pass through the numerous (F in south3、F5) north educate (F,1F2、F4、F6), sick 4 generation of garden is mixed in artificial withered and yellow wither
(F1、F2、F4、F6) stress screening, to obtain the disease resistance Early maturity (breeding time≤128 day) stable compared with strong, character, disease-resistant
(wilt disease refers to that≤10, verticillium wilt refers to≤20), high yield (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede length >=29mm, than
By force >=30cN/tex, mic value=3.5-5.0) New cotton line F6Generation.
4, variety test of lines
In production field, by the Early maturity of above-mentioned screening, disease-resistant, high yield, high-quality cotton new lines F6In generation, carries out strain comparison
Test (F7-F8Generation), further identify strain Early maturity (breeding time≤128 day), high yield [pre-frost lint yield than control (when
Ground main breed) volume increase >=5.0%], it is high-quality (suede length >=29mm, than by force >=30cN/tex, mic value=3.5-5.0)
Stability.
The breeding of embodiment 1, Early maturity disease-resistant high yield high-quality cotton new lines the Liao Dynasty 6999
Early maturity disease-resistant high yield high-quality cotton new lines the Liao Dynasty 6999 is breeding in accordance with the following steps:
1) the disease-resistant high yield high-quality cotton backbone parent of Early maturity is screened
In northeast, cotton planting district is excellent by the disease-resistant high yield of Early maturity using the cotton resource (i.e. distant cotton) planted for many years
The standards of grading (referring to table 2) and appraisement system (referring to table 1) of matter the Liao Dynasty cotton select the disease-resistant good cotton product of high yield of Early maturity
Kind is used as backbone parent；
It selects, backbone parent the Liao Dynasty cotton 31 (breeding time 128 days；Wilt disease refers to 2.2, and verticillium wilt refers to 17.1；Bell weight 6.6g,
Ginning outturn 42.1%, single basal munure 18 open bell number 16, wadding；The long 30.6mm of suede, than strong 30.8cN/tex, mic value 3.8；
General combining ability 1.26) and draw outside bell more (single basal munures 20), bell big (bell weighs 7.3g), Gao Yifen (ginning outturn 44.1%),
Close letter in the high-quality cotton resource Xinjiang (the long 29.4mm of suede, than strong 30.1cN/tex, mic value 5.1).
2) preparing hybrid combines
2012, the distant cotton 31 of sick garden plantation above-mentioned " backbone parent " was mixed and source Xinjiang conjunction of bringing in funds outside in manually withered and yellow wither
Letter." backbone parent " and cotton resource disease resistance good (not falling ill), growth potential are selected in full-bloom stage according to character principle of complementarity
Strong single plant, preparing hybrid combination guarantee that the hybridization bell obtained just has good disease resistance.
3) the disease-resistant high yield high-quality cotton new lines screening of offspring's Early maturity
(a) sick this combined hybrid bell of garden plantation (closing letter × the Liao Dynasty cotton 31 in Xinjiang) is mixed in artificial withered and yellow wither within 2013, from
It hands over and obtains F1In generation, identifies F1The disease resistance in generation.Screening disease resistance good (not falling ill), Early maturity (opening bell number 16, wadding or more), knot
The F of bell more (single basal munures 18 or more)1For single plant, and in remaining F1For all being received on all excellent (do not fall ill, bell more) single plants
1 middle part bell is obtained, guarantees F1It is all harvested for superior genotypes.
(b) it mixes sick garden in artificial withered and yellow wither and continues to plant the F1 generation single plant of said combination harvest and mixed receive within 2014
Whole seeds (that is, the middle part bell harvested on above-mentioned screening single plant), selfing obtain F2Generation separation single plant, further identification is each
The disease resistance of single plant, and screen disease resistance good (not falling ill), Early maturity (opening bell number 16, wadding or more), knot more (the single basal munures of bell
18 or more) fine individual plant.
(c) winter in 2014, by the F of above-mentioned selection2It is planted for fine individual plant seed in Hainan, selfing obtains F3Dai Zhu
Row, spring in 2015 screen F in Hainan3For excellent (neat, open wadding more) plant.
(d) spring in 2015, the F that will be screened in Hainan3Sick garden is mixed in northern artificial withered and yellow wither for excellent plant seed
Plantation, selfing obtain F4For strain, the disease resistance of strain is identified.Screening disease resistance it is good (wilt disease refers to≤10, verticillium wilt refers to≤
20), Early maturity (breeding time≤128 day), high yield (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede length >=29.0mm, than
By force >=30.0cN/tex, mic value 3.5-5.0；) elite plant strain, harvesting screened strain, to carry out south again numerous.
(e) winter in 2015, by the F of above-mentioned screening4It is planted for elite plant strain seed in Hainan, selfing obtains F5Dai Zhu
System, spring in 2016 screen F in Hainan5For excellent (neat, open wadding more) strain.
(f) 2016, the F that will be screened in Hainan5Sick garden plantation is mixed in northern artificial withered and yellow wither again for elite plant strain,
Selfing obtains F6For strain, continue the disease resistance for identifying strain.Autumn filters out disease resistance, and good (wilt disease refers to≤10, verticillium wilt
Refer to≤20), Early maturity (breeding time≤128 day), high yield (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede is long >=
29.0mm, than strong >=30.0cN/tex, mic value 3.5-5.0) excellent strain be new lines 6999 that breeding obtains.
Pass through F in above-mentioned breeding process1、F2、F4And F6Four generations it is artificial it is withered and yellow wither mixed this combination of raw Disease garden identification and
The disease resistance of its offspring ensure that selected F6There is stronger disease resistance for strain 6999.
4) variety test of lines
The F that (f) step is obtained6It is planted in production field for excellent strain, obtains F7For plant, its character is identified, the product
Yielding ability, prematureness and the fiber quality of system, distant 111 days 6999 breeding times；Flower rate 90.9% before frost.Plant type is moderate, plant height
81.3 centimetres, the first 24.3 centimetres of fruit branch height.Bell oval, single plant averagely tie 18.7, bell, moderate containing wadding a quilt with cotton, and easily pick.Leaf
Color depth is green.Wilt disease refers to 1.4, and verticillium wilt refers to 0.8, highly resistance blight.Upper half mean length 30.4mm, uniformity index
84.3%, strength 29.3cN/tex, mic value 4.9, elongation 6.7%, fiber quality is excellent.Average bell weight
6.3g, ginning outturn 44.3%.Unginned cotton and gined cotton per mu yield before frost are 228.3kg and 101.1kg respectively before frost, than compareing distant cotton No. 23
Increase production 6.4% and 17.8% respectively, yielding ability is good.
The breeding of embodiment 2, Early maturity disease-resistant high yield high-quality cotton new lines the Liao Dynasty 5449
Early maturity disease-resistant high yield high-quality cotton new lines the Liao Dynasty 5449 is breeding in accordance with the following steps:
(a) the sick garden plantation (F of remote 321) of distant No. 9 × stone of cotton is mixed in artificial withered and yellow wither within 20104For material (verticillium wilt
Refer to 20.3；Bell weight 5.8g, ginning outturn 40.5%, single basal munure 15 open bell number 12, wadding；The long 28.0mm of suede, than strong 29.2cN/
Tex, mic value 4.6) and the disease-resistant high yield of Early maturity high-quality backbone parent the Liao Dynasty cotton No. 27 (breeding time 128 days；Wilt disease refers to
0.3, verticillium wilt refers to 12.4；Bell weight 6.7g, ginning outturn 41.6%, single basal munure 17 open bell number 14, wadding；The long 30.2mm of suede, than
Strong 31.8cN/tex, mic value 4.4；General combining ability 1.18), disease resistance good (not falling ill), growing way are selected in full-bloom stage
Strong single plant preparing hybrid combination, guarantees that the hybridization bell obtained just has good disease resistance.
This combination is the example using backbone parent improvement filial generation material.
(b) 2011 it is artificial it is withered and yellow wither mix sick garden plantation [(distant No. 9 × stone of cotton remote 321) × the Liao Dynasty cotton No. 27] this
Combined hybrid bell, selfing obtain F1In generation, identifies F1The disease resistance in generation.Screening disease resistance good (not falling ill), Early maturity (open wadding bell number
14 or more), the single plant of knot bell more (single basal munures 17 or more), and in remaining F1It is single for all excellent (do not fall ill, bell more)
1 middle part bell is all harvested in strain, guarantees F1It is all harvested for superior genotypes.
(c) 2012 in the artificial withered and yellow F for mixing and continuing to plant said combination harvest in sick garden that withers1For single plant and mix receipts
Whole seeds, selfing obtain F2Generation separation single plant, further identifies the disease resistance of each single plant, and screen that disease resistance is good (not to be sent out
Disease), the fine individual plant of Early maturity (open wadding bell number 14 or more), knot bell more (single basal munures 17 or more).
(d) winter in 2012, by the F of above-mentioned selection2It is planted for fine individual plant in Hainan, selfing obtains F3For plant, 2013
Spring in year screens F in Hainan3For excellent (neat, open wadding more) plant.
(e) 2013, the F that will be screened in Hainan3Sick garden plantation F is mixed in artificial withered and yellow wither for excellent plant4For strain,
It identifies the disease resistance of strain, screens disease resistance good (wilt disease refers to≤10, and verticillium wilt refers to≤20), Early maturity (breeding time≤128
It), high yield (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede length >=29.0mm, than by force >=30.0cN/tex, mic value
3.5-5.0；) elite plant strain carry out south it is numerous.
(f) winter in 2013, by the F of above-mentioned screening4It is planted for elite plant strain in Hainan, selfing obtains F5For strain, 2014
Spring in year screens F in Hainan5For excellent (neat, open wadding more) strain.
(g) 2014, the F that will be screened in Hainan5Sick garden plantation is mixed in artificial withered and yellow wither for elite plant strain, selfing obtains
Obtain F6For strain, continue the disease resistance for identifying strain.Autumn filter out disease resistance it is good (wilt disease refers to≤10, verticillium wilt refers to≤
20), Early maturity (breeding time≤128 day), high yield (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede length >=29.0mm, than
By force >=30.0cN/tex, mic value 3.5-5.0) excellent strain 5449.By F1、F2、F4And F6In four generations, are artificial withered and yellow
It withers and mixes the disease resistance of this combination of raw Disease garden identification and its offspring, ensure that selected F6Have for strain 5449 stronger disease-resistant
Property.
(h) 2015, in production field, above-mentioned selected strain 5449 is subjected to variety test of lines, identifies F7For strain
Yielding ability, prematureness and fiber quality, autumn filters out Early maturity (breeding time≤128 day), high yield (pre-frost lint yield
Than compareing No. 23 volume increase >=5% of distant cotton), high-quality (suede length >=29.0mm, than by force >=30.0cN/tex, mic value 3.5-5.0)
Excellent strain 5449.
(i) variety comparative test, 2015-2016 set 12 points of test average result in Liaoning Province: distant 5449 plant types are suitable
In, 78 centimetres of plant height, the first 22.7 centimetres of fruit branch height.Bell oval, single plant averagely tie 12.9, bell.Containing moderate, the Yi Cai that wads a quilt with cotton
It plucks.Dark green leaf color.Breeding time 120 days, rate 93.0% was spent before white.Wilt disease refers to 6.6, and verticillium wilt refers to 7.7, anti-blight, highly resistance
Verticillium wilt.Upper half mean length 29.5mm, than strong 30.1cN/tex, mic value 4.6, fiber quality is excellent.Average bell weight
6.4g, ginning outturn 43.1%.Unginned cotton and gined cotton per mu yield before frost are 215.6kg and 92.9kg respectively before frost, than compareing distant No. 23 points of cotton
Not Zeng Chan 5.4% and 13.0%, yielding ability is good.
(j) Liaoning Province's Regional Test of Cotton Varieties result in 2017:
(1) feature: breeding time 126 days；Flower rate 90.06% before frost.Plant height 76.3cm, plant height is moderate, tower
Compact, the first fruit branch height is 26.5cm.Medium blade is slightly larger, and leaf color is deeper.Bell oval, blow-of-cottons are smooth, wad a quilt with cotton white.Fruit branch 8.3
Platform, boll per plant 11.4, single bell seed cotton heavy 6.33g, ginning outturn 40.74%, seed refers to that 9.60g, seed size are moderate.
Disease Resistance Identification result: wilt disease index is 2.43 (HR), high resistance to wilt good；Verticillium wilt refers to as 11.93 (R), resists
Verticillium wilt.
Fiber quality testing result: the long 31.8mm of suede, uniformity index 86.1%, strength 31.7cN/tex, horse
Clone's value 4.9, elongation 6.8%.Overall target reaches the standard of II type routine cotton variety.
Yield performance: gined cotton output per hectare 946.60kg before frost, than control volume increase 7.19%；Unginned cotton's output per hectare
2576.39kg than control volume increase 6.00%；Gined cotton output per hectare 1049.87kg, than control volume increase 9.26%.Gined cotton before frost
5 points of volume increase.
(2) distant cotton 46 (the Liao Dynasty 5449) feature: plant height is moderate, and tower is compacter, and medium blade is slightly larger, and leaf color is deep.Bell
Oval, seed size is moderate, and blow-of-cottons are smooth, wads a quilt with cotton white, ginning outturn is preferable, and fiber quality is excellent.Prematureness is good, knot bell habit, high yield
Property, disease resistance are good.
(3) conclusion: the strain meets product of the II type normal conon New cotton line regional testing than control volume increase >=3.0%
Kind authorization codes and standards, and general performance is excellent.It is recommended that continuing to participate in succeeding year regional testing.
Embodiment 3
Difference from Example 1 is:
Different backbone parents is obtained according to the Content Selection recorded in Tables 1 and 2, and source of bringing in funds outside, then according to
Above-mentioned breeding mode obtains cross combination F1Generation (referring to table 3), combination acceptance rate are higher (up to 50%).(table 3 is to utilize backbone
The cross combination that parent prepares with source of bringing in funds outside according to character principle of complementarity, combination acceptance rate is 50%；Table 4 is general parent
This cross combination prepared according to character principle of complementarity, combination acceptance rate is 40.5%.)
Embodiment 4
Difference from Example 1 is:
Different backbone parents is obtained according to the Content Selection recorded in Tables 1 and 2, and source of bringing in funds outside, then according to
Above-mentioned breeding mode obtains cross combination F1In generation, screens F according still further to above-mentioned breeding method2—F6The progeny materials such as generation, and then obtain
Different new lines (referring to table 5).
The table 3 2017 years cross combination F using backbone parent and source preparation of bringing in funds outside1For variable rate technology, F1In generation, combines excellent
Yield=11/22=50.0%
Note: indicate that group " well early " is combined into excellent combination in " combination variable rate technology " column in table 3；It is indicated in this column
The group of " general or superseded " is combined into general or bad combination.
4 2017 years general cross of table combine F1For variable rate technology, F1Generation combination acceptance rate=49/121=40.5%
Note: indicate that group " well early " is combined into excellent combination in " combination variable rate technology " column in table 4；It is indicated in this column
The group of " general or superseded " is combined into general or bad combination.
It is excellent that table 5 utilizes " the disease-resistant high-quality backbone parent of high yield of Early maturity and source of bringing in funds outside " preparing hybrid combined sorting to go out
Strain
The above description is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all utilizations
Equivalent structure transformation made by present specification is applied directly or indirectly in other relevant technical fields, same
Reason is included within the scope of the present invention.
Claims (5)
1. a kind of disease-resistant High-Efficient Cotton breeding method of Early maturity, it is characterised in that:
1) screening obtains the backbone parent of cotton Early maturity breeding for disease resistance: in northeast, cotton planting district utilizes the cotton planted for many years
Resource selects the disease-resistant high yield of Early maturity good by the standards of grading and appraisement system of the high-quality distant cotton of the disease-resistant high yield of Early maturity
Cotton variety is as backbone parent；
2) hybridize the acquisition of bell: mixing sick garden in artificial withered and yellow wither and plant that the backbone parent and bell are more, bell is big, Gao Yifen, excellent
The outer of matter draws cotton resource, and according to character principle of complementarity, in full-bloom stage, hybridization obtains F0Generation hybridization bell；
3) numerous (F in south the acquisition of the disease-resistant high yield high-quality cotton new lines of Early maturity: is passed through using pedigree method3Generation and F5Generation) north educates (F1
Generation, F2Generation, F4Generation and F6Generation, and sick garden F is mixed in artificial withered and yellow wither1Generation, F2Generation, F4Generation and F6In 4 generations in generation, coerce screening), from
And obtain the disease resistance Early maturity (breeding time≤128 day) stable compared with strong, character, it is disease-resistant (wilt disease refer to≤10, verticillium wilt refer to≤
20), get bumper crops (5 meter Hang Chang seed cotton yields >=0.9kg), it is high-quality (suede length >=29mm, than by force >=30cN/tex, mic value=
3.5-5.0)F6For New cotton line.
2. the disease-resistant High-Efficient Cotton breeding method of Early maturity according to claim 1, it is characterised in that: the step 2) is by step
1) obtain backbone parent with draw cotton resource preparing hybrid outside and combine, hybridize acquisition F0Generation hybridization bell, then by the hybridization of acquisition
Bell is planted in artificial withered and yellow wither and mixes sick garden selfing acquisition F1For plant, to F1It is screened for cross combination, harvests Early maturity
(breeding time≤128 day), disease-resistant (wilt disease refers to that≤10, verticillium wilt refers to≤20), high yield (5 meter Hang Chang unginned cotton's weight >=0.9kg)
Cross combination, for use.
3. the disease-resistant High-Efficient Cotton breeding method of Early maturity according to claim 1, it is characterised in that: by above-mentioned screened F1
Sick garden plantation is mixed in artificial withered and yellow wither for cross combination, selfing obtains F2Generation separation single plant, screening Early maturity (breeding time≤
128 days), disease-resistant (not falling ill), high yield (single basal munure >=18, open wadding bell number >=15), high-quality (suede length >=29mm) F2Generation
Single plant, for use；
Above-mentioned screening is obtained into F2It is planted in winter current year in Hainan for single plant, selfing obtains F3For plant, screening is excellent (neatly,
Open and wad a quilt with cotton) plant；
F will be obtained3Sick garden plantation is mixed in northern artificial withered and yellow wither again in spring for plant, selfing obtains F4For strain, screening is special
Precocious (breeding time≤128 day), disease-resistant (wilt disease refers to that≤10, verticillium wilt refers to≤20), high yield (5 meter Hang Chang seed cotton yields >=
0.9kg), high-quality (suede length >=29mm, than strong >=30cN/tex, mic value=3.5-5.0) strain；
The strain of above-mentioned screening is planted in winter in this year in Hainan, selfing obtains F5For strain；
By excellent (neatly, open and the wad a quilt with cotton) F of the numerous acquisition in above-mentioned south5Sick garden plantation is mixed in spring to northern artificial withered and yellow wither for strain,
Selfing obtains Early maturity (breeding time≤128 day), disease-resistant (wilt disease refers to that≤10, verticillium wilt refers to≤20), high yield (5 meters of row eldest sons
Ginnings >=0.9kg), high-quality (suede length >=29mm, than strong >=30cN/tex, mic value=3.5-5.0) the stable F of character6
For strain, the i.e. disease-resistant high yield high-quality cotton new lines of Early maturity.
4. the disease-resistant High-Efficient Cotton breeding method of Early maturity according to claim 1, it is characterised in that: described anti-by Early maturity
The standards of grading and appraisement system of the high-quality distant cotton of disease high yield, select the backbone parent of the disease-resistant high yield high-quality cotton of Early maturity for distant cotton
No. 23, distant cotton No. 27, distant cotton 31 or distant cotton 34；
The bell is more, bell is big, Gao Yifen, the good outer cotton resource that draws are that stone is remote 321, letter, Ji Mian 169, middle plant cotton 2 are closed in Xinjiang
Number, Xinluzao No.1, R001, HGBMHR-H, Xinjiang KW-37, P10-1, Shanxi 0316 or 14244612.
5. by the disease-resistant High-Efficient Cotton breeding method of Early maturity described in claim 1-4, it is characterised in that: the kind of the screening
Technical indicator:
(1) Reproduction period index: Exceptional early mature cotton region≤128 day such as Liaoning；
(2) fiber quality traits: I type: suede length >=31mm, specific strength >=32cN/tex, mic value 3.7-4.2；II type: suede is long
>=29mm, specific strength >=30cN/tex, mic value 3.5-5.0；III type: suede length >=27mm, specific strength >=28cN/tex, mark
Grand value 3.5-5.5；
(3) yield index: I type: pre-frost lint yield is than local main breed (the Liao Dynasty 23) volume increase >=0.0%；II type: gined cotton before frost
Yield is than local main breed (the Liao Dynasty 23) volume increase >=3.0%；III type: pre-frost lint yield is than local main breed (the Liao Dynasty 23) volume increase
>=8.0%.Ginning outturn reaches 40% or more；Flower rate >=85% before frost；
(4) Resistance index: wilt disease refers to≤20, and verticillium wilt refers to≤35.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110150056A (en) * | 2019-07-01 | 2019-08-23 | 上海市农业科学院 | A kind of seed growing method keeping anti-blight variety of watermelon resistance |
| CN110169355A (en) * | 2019-07-04 | 2019-08-27 | 江苏省农业科学院宿迁农科所 | A kind of breeding method of summer live streaming cotton variety |
| CN112349351A (en) * | 2020-11-10 | 2021-02-09 | 邯郸市农业科学院 | Method for breeding ultra-early-maturing cotton germplasm with assistance of molecular markers |
| CN113498734A (en) * | 2021-06-29 | 2021-10-15 | 辽宁省经济作物研究所 | Non-transgenic cotton breeding method |
| CN117084165A (en) * | 2023-09-11 | 2023-11-21 | 新疆农垦科学院 | Breeding method of early-maturing high-yield hybrid cotton |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110150056A (en) * | 2019-07-01 | 2019-08-23 | 上海市农业科学院 | A kind of seed growing method keeping anti-blight variety of watermelon resistance |
| CN110169355A (en) * | 2019-07-04 | 2019-08-27 | 江苏省农业科学院宿迁农科所 | A kind of breeding method of summer live streaming cotton variety |
| CN110169355B (en) * | 2019-07-04 | 2020-12-04 | 江苏省农业科学院宿迁农科所 | Cultivation method of summer direct seeding cotton variety |
| CN112349351A (en) * | 2020-11-10 | 2021-02-09 | 邯郸市农业科学院 | Method for breeding ultra-early-maturing cotton germplasm with assistance of molecular markers |
| CN113498734A (en) * | 2021-06-29 | 2021-10-15 | 辽宁省经济作物研究所 | Non-transgenic cotton breeding method |
| CN117084165A (en) * | 2023-09-11 | 2023-11-21 | 新疆农垦科学院 | Breeding method of early-maturing high-yield hybrid cotton |
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