CN109364132B - Method for extracting effective components from prinsepia utilis royle - Google Patents
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Abstract
The invention discloses a method for extracting effective components from prinsepia utilis royle, wherein the method for extracting the effective components from the prinsepia utilis royle takes dried prinsepia utilis royle as a raw material, the prinsepia utilis royle oil components are extracted through a cold squeezer, the coarse oil is subjected to high-speed centrifugation, decoloration and low-temperature freezing separation to remove macromolecular oil, and the micromolecular essential oil is dehydrated to obtain micromolecular prinsepia utilis royle essential oil; extracting the squeezed prinsepia utilis royle residues with ethanol, concentrating the extract, recovering the ethanol, adsorbing the prinsepia utilis royle total alkali by using a low-alcohol-soluble acrylic acid weak-acid cation resin column, eluting the prinsepia utilis royle alkaloid by using an ethanol solution containing ammonia water, and obtaining the prinsepia utilis royle total alkali and dehydrated small-molecular prinsepia utilis royle oil which is the prinsepia utilis royle extract; the extract is used for preparing Prinsepia utilis Royle extract gel preparation; the method is simple, easy to operate, high-efficiency and suitable for industrial production and market popularization and application.
Description
Technical Field
The invention relates to the field of preparation of extracts and preparations, in particular to a method for extracting effective components from prinsepia utilis royle.
Background
The Prinsepia utilis Royle is a perennial rare woody oil plant, grows in hillside, wasteland, land, roadside and irrigation clusters of yin-wet mountain ditches with the altitude of 1800-3000 m, is particularly suitable for growing in the land with the altitude of 2200-2700 m, and is an economic plant with medicine and food dual purposes.
The Prinsepia utilis Royle oil contains Prinsepia utilis Royle oil which mainly contains unsaturated fatty acid, oleic acid, linoleic acid, palmitic acid, vitamin A, vitamin D, 1 vitamin E, vitamin K and the like, and also contains abundant major and trace elements such as calcium, sulfur, manganese, magnesium, potassium, phosphorus, zinc, iron and the like, the content of the fatty acid, the oleic acid and the linoleic acid in the Prinsepia utilis Royle oil is about 30 percent, the Prinsepia utilis Royle oil can be converted into arachidonic acid in vivo to be a biochemical medium for transmitting intercellular information, and is related to inflammation, infection, tissue injury and immune system regulation, the Prinsepia utilis Royle oil has the effects of relieving hypertension and resisting atherosclerosis formation when being taken orally, and the Prinsepia utilis Royle oil has the effects of resisting oxidation, resisting inflammation; the Prinsepia utilis Royle oil preparation is reported to have outstanding natural permeability in vitro experiments and is used in skin and tissues, 60 percent of nutrients can enter deep tissues, 40 percent of nutrients can enter dermis, and basically no nutrients can be lost; the Prinsepia utilis Royle oil is traditionally used for snake and insect bites, skin care and the like, and has good effects.
The prinsepia utilis royle fruit contains a small amount of alkaloid besides the prinsepia utilis royle fruit oil, the total alkaloids of the prinsepia utilis royle fruit are large in polarity and have certain water solubility, are main anti-inflammatory components in the prinsepia utilis royle fruit, the anti-inflammatory effect of the total alkaloids is higher than that of the prinsepia utilis royle fruit oil, and the total alkaloids of the prinsepia utilis royle fruit have stronger prefabricated effect on pathogenic bacteria such as staphylococcus aureus, escherichia coli, shigella and candida albicans.
The traditional processing method of the prinsepia utilis royle fruit mainly extracts prinsepia utilis royle fruit oil, oil residues are discarded, the prinsepia utilis royle fruit oil obtained by a squeezing method contains a small amount of water, a large amount of high-molecular fatty acid and a small amount of protein, and the obtained oil is easy to generate a layering corruption phenomenon after being placed for a long time. And the research on the extraction of the total alkaloid of the prinsepia utilis royle is few and less.
Disclosure of Invention
The invention aims to provide a method for extracting effective components from prinsepia utilis royle, which solves the problem of high difficulty in extracting total alkaloids of the prinsepia utilis royle.
In order to solve the technical problems, the invention adopts the following technical scheme:
a method for extracting effective components from Prinsepia utilis Royle comprises the following steps:
squeezing: squeezing the Prinsepia utilis royle dry powder at low temperature to obtain Prinsepia utilis royle dregs and Prinsepia utilis royle crude oil;
extraction: reflux-extracting the prinsepia utilis royle dregs with ethanol as a solvent to obtain an ethanol crude extract;
and (3) purification: filtering the crude ethanol extract, adsorbing total alkaloids in the crude ethanol extract by using acidic cation resin, washing the column by using water until effluent is colorless, and eluting the total alkaloids adsorbed in the acidic cation resin by using ethanol solution containing ammonia water to obtain total alkali solution;
concentration: and adjusting the pH value of the total alkali solution to 6.8-7.2, and concentrating to obtain the prinsepia utilis royle total alkali extract.
Preferably, in the extraction step, the volume concentration of the ethanol is 40-70%, and in the purification step, the volume concentration of the ethanol is 40-50%.
Preferably, the concentration step is followed by refining, wherein the refining is to dissolve the prinsepia utilis royle total alkali extract in ethanol, then to stand at-5 ℃ until the insoluble substances do not increase any more, to filter the insoluble substances, and then to concentrate the insoluble substances to obtain the refined prinsepia utilis royle total alkali.
Preferably, the ethanol solution containing ammonia water is formed by mixing ammonia water and 75-85% of ethanol solution by mass concentration, wherein the mass concentration of the ammonia water in the solution is 8-12%.
Preferably, the crude Prinsepia utilis royle oil is separated, frozen and dried to obtain the small molecular Prinsepia utilis royle oil, wherein the freezing is to freeze the centrifuged crude Prinsepia utilis royle oil at-10 to-15 ℃ for 20 to 30 hours and then filter the frozen crude Prinsepia utilis royle oil.
Preferably, the separation step is to centrifuge the crude Prinsepia utilis royle oil at a centrifugation speed of 10000-25000 r/min for 1-1.5 h at a temperature of-12-4 ℃, and then adsorb adsorbable components in the crude Prinsepia utilis royle oil by using activated carbon.
Preferably, the drying is to mix the centrifuged prinsepia utilis royle oil and anhydrous sodium sulfate and stir for 1-2 hours, wherein the anhydrous sodium sulfate accounts for 6-12 wt% of the prinsepia utilis royle oil.
A Prinsepia utilis royle extract gel comprises a Prinsepia utilis royle extract solution and a matrix, wherein the volume ratio of the Prinsepia utilis royle extract solution to the matrix is 10-15: 85-90, and the Prinsepia utilis royle extract solution comprises 7-8 parts by weight of the dehydrated small-molecular Prinsepia utilis royle oil disclosed in any one of claims 5-7 and 2-3 parts by weight of the Prinsepia utilis royle total alkali disclosed in any one of claims 1-7.
Preferably, the prinsepia utilis royle extract solution further comprises 0.4-0.6 parts by weight of borneol, 0.02-0.04 parts by weight of essence and 0.4-0.6 parts by weight of glycerol, the matrix is carbomer gel with the mass concentration of 0.5-0.8%, and the mass concentration of sodium benzoate in the carbomer gel is 0.1-0.3%.
A method for processing the Prinsepia utilis Royle extract gel according to claim 8 or 9,
(1) dissolving carbomer 941 powder and sodium benzoate in purified water, uniformly stirring for swelling, and standing to form a carbomer gel solution with the mass concentration of 0.5-0.8%, wherein the mass concentration of the sodium benzoate in the water is 0.1-0.3%;
(2) mixing the dehydrated small molecular Prinsepia utilis Royle oil of any one of claims 5 to 7, the total Prinsepia utilis Royle alkali of any one of claims 1 to 7, borneol, essence and glycerol according to the mass ratio of 7-8: 2-3: 0.4-0.6: 0.02-0.04: 0.4-0.6, and stirring for dissolving to obtain a Prinsepia utilis Royle extract solution;
(3) adding the prinsepia utilis royle extract solution obtained in the step (2) into the carbomer gel solution obtained in the step (1), wherein the volume ratio of the prinsepia utilis royle extract solution to the carbomer gel solution is 10-15: 85-90, then placing the mixture into a homogenizer, adding triethanolamine while stirring to adjust the pH value to be 7.2-6, taking out the mixture, and performing ultrasonic degassing to obtain the prinsepia utilis royle extract gel.
The method adopts a high-speed centrifugation and low-temperature freezing method to precipitate and remove high-molecular fatty acid and protein at low temperature, retains beneficial prinsepia utilis royle oil such as oleic acid, linoleic acid and unsaturated fatty acid, adopts sodium sulfate to dehydrate and remove residual water, and refines to ensure that the prinsepia utilis royle oil has better activity and purity, and the obtained oil does not deteriorate and delaminate after long-term storage and has better property; the method is simple, easy to operate, high-efficiency and suitable for industrial production and market popularization and application.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1:
the preparation method of the prinsepia utilis royle extract comprises the following steps
(1) Crushing 1kg of dried prinsepia utilis royle, putting into a low-temperature squeezer, and squeezing at low temperature of 4 ℃ to obtain prinsepia utilis royle crude oil;
(2) centrifuging the crude Prinsepia utilis Royle of the step (1) at a high speed of 10000 r/min for 1h by using a refrigerated centrifuge, and allowing supernate to pass through an active carbon column to obtain light yellow Prinsepia utilis Royle oil;
(3) freezing the prinsepia utilis royle oil obtained in the step (2) at-12 ℃ for 24 hours to generate layering, wherein the lower layer is macromolecular oily solid matter, the upper layer is micromolecular oil, and filtering after freezing to obtain micromolecular prinsepia utilis royle oil;
(4) adding anhydrous sodium sulfate accounting for 10% of the mass of the small molecule prinsepia utilis royle oil in the step (3) to adsorb residual water in the oil, magnetically stirring for 1h, and performing suction filtration to remove the sodium sulfate to obtain 160g of dehydrated small molecule prinsepia utilis royle oil;
(5) crushing the prinsepia utilis royle dregs obtained after low-temperature pressing in the step (1), performing hot reflux extraction for 2 times by using an ethanol solution with the volume concentration of 65%, combining the extracting solutions, and recovering ethanol to obtain an prinsepia utilis royle ethanol crude extract;
(6) adding ethanol into the ethanol crude extract of Prinsepia utilis Royle of step (5), stirring for dissolving, wherein the ethanol content in the solution is 40%, filtering to remove insoluble substances, passing the filtrate through acrylic acid weakly acidic cation resin 151 column for adsorbing total alkaloids, washing the column with water after complete adsorption until the effluent is colorless, then, eluting the total alkaloids by using an ethanol solution containing ammonia water (the ethanol solution containing ammonia water is prepared by adding the ammonia water into an ethanol solution with the mass concentration of 75%, the mass percentage concentration of the ammonia water in the solution is 8%), identifying the bismuth potassium iodide, collecting the eluent containing the total alkaloids of the prinsepia utilis royle to obtain the total alkaloids solution of the prinsepia utilis royle, adjusting the pH value of the total alkaloids solution of the prinsepia utilis royle to be neutral by hydrochloric acid, concentrating and recovering ethanol to obtain the total alkaloids extract of the prinsepia utilis royle, drying the extract, thermally dissolving the extract by using absolute ethanol, then standing at 0 ℃ for 8h, filtering out insoluble ammonium chloride, recovering ethanol from the filtrate, and drying to obtain 6.3g of Prinsepia utilis total alkali;
(7) the prinsepia utilis royle total alkali and the dehydrated small-molecular prinsepia utilis royle oil are prinsepia utilis royle extracts;
(8) dissolving carbomer 941 powder and sodium benzoate in purified water, stirring to swell uniformly, standing for 8 hours to form a carbomer gel solution with the mass concentration of 0.5%, wherein the mass concentration of sodium benzoate in water is 0.2%;
(9) mixing dehydrated small molecular Prinsepia utilis Royle oil, Prinsepia utilis Royle total alkali, Borneolum Syntheticum, essence and glycerol at a mass ratio of 8:2:0.5:0.03:5, and stirring for dissolving to obtain Prinsepia utilis Royle extract solution;
(10) adding the prinsepia utilis royle extract solution into the carbomer gel solution, wherein the volume ratio of the prinsepia utilis royle extract solution to the carbomer gel solution is 15:85, then placing the mixture into a homogenizer, adding triethanolamine while stirring to adjust the pH value to 7.4, taking out the mixture, performing ultrasonic degassing, and subpackaging to obtain the prinsepia utilis royle extract gel preparation.
Example 2:
the preparation method of the prinsepia utilis royle extract comprises the following steps
(1) Crushing 1kg of dried prinsepia utilis royle, putting into a low-temperature squeezer, and squeezing at low temperature of 2 ℃ to obtain prinsepia utilis royle crude oil;
(2) centrifuging the crude Prinsepia utilis Royle of the step (1) at a high speed of 15000 r/min for 1.2h by using a refrigerated centrifuge, and allowing the supernatant to pass through an active carbon column to obtain light yellow Prinsepia utilis Royle oil;
(3) freezing the prinsepia utilis royle oil in the step (2) at-10 ℃ for 20 hours, layering, and forming a macromolecular oily solid on the lower layer
The upper layer is micromolecular oil, and the micromolecular prinsepia utilis royle oil is obtained by filtering after freezing;
(4) adding anhydrous sodium sulfate with the mass of 6% of the small molecule prinsepia utilis royle oil in the step (3) to adsorb residual water in the oil, magnetically stirring for 1.5h, and performing suction filtration to remove the sodium sulfate to obtain 176g of dehydrated small molecule prinsepia utilis royle oil;
(5) crushing the prinsepia utilis royle dregs obtained after low-temperature pressing in the step (1), performing hot reflux extraction for 3 times by using an ethanol solution with the volume concentration of 50%, combining the extracting solutions, and recovering ethanol to obtain an prinsepia utilis royle ethanol crude extract;
(6) adding ethanol into the ethanol crude extract of Prinsepia utilis Royle of step (5), stirring for dissolving, wherein the ethanol content in the solution is 45%, filtering out insoluble substances, passing the filtrate through acrylic acid weakly acidic cation resin 151 column for adsorbing total alkaloids, washing the column with water after complete adsorption until the effluent is colorless, then, using an ethanol solution containing ammonia water (the ethanol solution containing ammonia water is prepared by adding the ammonia water into an ethanol solution with the mass concentration of 80%, the mass percentage concentration of the ammonia water in the solution is 10%) to elute the total alkaloids, identifying the bismuth potassium iodide, collecting the eluent containing the total alkaloids of the prinsepia utilis royle to obtain the total alkaloids solution of the prinsepia utilis royle, adjusting the pH value of the total alkaloids solution of the prinsepia utilis royle to be neutral by hydrochloric acid, concentrating and recovering ethanol to obtain the total alkaloids extract of the prinsepia utilis royle, drying the extract, then using absolute ethanol to carry out thermal, then standing at 4 ℃ for 8h, filtering out insoluble ammonium chloride, recovering ethanol from the filtrate, and drying to obtain 6.6g of Prinsepia utilis total alkali;
(7) the prinsepia utilis royle total alkali and the dehydrated small-molecular prinsepia utilis royle oil are prinsepia utilis royle extracts;
(8) dissolving carbomer 941 powder and sodium benzoate in purified water, stirring to swell uniformly, standing for 8 hours to form a carbomer gel solution with the mass concentration of 0.7%, wherein the mass concentration of sodium benzoate in water is 0.1%;
(9) mixing dehydrated small molecule prinsepia utilis royle oil, prinsepia utilis royle total alkali, borneol, essence and glycerol according to the mass ratio of 7:3:0.4:0.02: 0.4, and stirring for dissolving to obtain prinsepia utilis royle extract solution;
(10) adding the prinsepia utilis royle extract solution into the carbomer gel solution, wherein the volume ratio of the prinsepia utilis royle extract solution to the carbomer gel solution is 10:90, then placing the mixture into a homogenizer, adding triethanolamine while stirring to adjust the pH value to 7.4, taking out the mixture, performing ultrasonic degassing, and subpackaging to obtain the prinsepia utilis royle extract gel preparation.
Example 3:
the preparation method of the prinsepia utilis royle extract comprises the following steps
(1) Crushing 1kg of dried prinsepia utilis royle, putting into a low-temperature squeezer, and squeezing at low temperature of 3 ℃ to obtain prinsepia utilis royle crude oil;
(2) centrifuging the crude Prinsepia utilis Royle of the step (1) at a high speed of 10000 r/min for 1.2h by using a refrigerated centrifuge, and allowing supernatant to pass through an active carbon column to obtain light yellow Prinsepia utilis Royle oil;
(3) freezing the prinsepia utilis royle oil obtained in the step (2) at-15 ℃ for 24 hours to generate layering, wherein the lower layer is macromolecular oily solid matter, the upper layer is micromolecular oil, and filtering after freezing to obtain micromolecular prinsepia utilis royle oil;
(4) adding anhydrous sodium sulfate accounting for 12% of the mass of the small molecule prinsepia utilis royle oil in the step (3) to adsorb residual water in the oil, magnetically stirring for 2 hours, and performing suction filtration to remove the sodium sulfate to obtain 248.1g of dehydrated small molecule prinsepia utilis royle oil;
(5) crushing the prinsepia utilis royle dregs obtained after low-temperature pressing in the step (1), performing hot reflux extraction for 2 times by using an ethanol solution with the volume concentration of 75%, combining the extracting solutions, and recovering ethanol to obtain an prinsepia utilis royle ethanol crude extract;
(6) adding ethanol into the ethanol crude extract of Prinsepia utilis Royle of step (5), stirring for dissolving, wherein the ethanol content in the solution is 50%, filtering to remove insoluble substances, passing the filtrate through acrylic acid weakly acidic cation resin 113 column for adsorbing total alkaloids, washing the column with water after complete adsorption until the effluent is colorless, then, using an ethanol solution containing ammonia water (the ethanol solution containing ammonia water is prepared by adding the ammonia water into an ethanol solution with the mass concentration of 85 percent, the mass percentage concentration of the ammonia water in the solution is 11 percent) to elute the total alkaloids, identifying the bismuth potassium iodide, collecting the eluent containing the total alkaloids of the prinsepia utilis royle to obtain the total alkaloids solution of the prinsepia utilis royle, adjusting the pH value of the total alkaloids solution of the prinsepia utilis royle to be neutral by hydrochloric acid, concentrating and recovering ethanol to obtain the total alkaloids extract of the prinsepia utilis royle, drying the extract, then using absolute ethanol to carry, standing at-5 deg.C for 8 hr, filtering off insoluble ammonium chloride, recovering ethanol from filtrate, and drying to obtain Prinsepia utilis total alkali 6.1 g;
(7) the prinsepia utilis royle total alkali and the dehydrated small-molecular prinsepia utilis royle oil are prinsepia utilis royle extracts;
(8) dissolving carbomer 941 powder and sodium benzoate in purified water, stirring to swell uniformly, standing for 8 hours to form a carbomer gel solution with the mass concentration of 0.7%, wherein the mass concentration of sodium benzoate in water is 0.3%;
(9) mixing dehydrated small molecular Prinsepia utilis Royle oil, Prinsepia utilis Royle total alkali, Borneolum Syntheticum, essence and glycerol at a mass ratio of 8:3:0.6:0.05:0.6, stirring and dissolving to obtain Prinsepia utilis Royle extract solution;
(10) adding the prinsepia utilis royle extract solution into the carbomer gel solution, wherein the volume ratio of the prinsepia utilis royle extract solution to the carbomer gel solution is 12:88, then placing the mixture into a homogenizer, adding triethanolamine while stirring to adjust the pH value to 7.4, taking out the mixture, performing ultrasonic degassing, and subpackaging to obtain the prinsepia utilis royle extract gel preparation.
The anti-inflammatory effect of the extracts of the above examples was verified by the mouse ear swelling method (in vitro experiment):
taking 40 Kunming mice, each half of male and female, randomly dividing into 4 groups, anesthetizing with ether, coating 100% xylene inflammation solution on the front and back surfaces of the right ear, 0.02 mL/mouse, dissolving Prinsepia utilis Royle total alkali with a small amount of normal saline to form suspension, and coating the inflammation part with normal saline in the blank group (group A); the Prinsepia utilis oil dosage group (group B) is applied to inflammation part at dosage of 10mg/kg containing Prinsepia utilis oil; the Prinsepia utilis royle alkaloid group (group C) is applied to the inflammation part at a dose of 2mg/kg containing Prinsepia utilis royle alkaloid; applying Prinsepia utilis oil and Prinsepia utilis alkaloid mixed group (group D) at the inflammation position with the dosage of Prinsepia utilis oil of 5.0mg/kg and Prinsepia utilis alkaloid of 1.5 mg/kg; after each group is coated with the medicine for 4 hours, the mouse is killed after being taken off the cervical vertebra, round holes are punched on the same positions of two ears of the mouse by a 5mm puncher, the mouse is weighed by an electronic balance, the swelling degree is calculated by reducing the weight of the right ear and the weight of the left ear, and the swelling rate is calculated; the following table shows that the prinsepia utilis royle oil and the prinsepia utilis royle alkaloid can obviously inhibit the mouse ear swelling caused by the dimethylbenzene, wherein the swelling rate of the group D is the lowest.
Table 1: effect of swelling rate (n = 10)
| Group of | Swelling degree (mg) | Swelling Rate (%) |
| Group A | 7.6±1.2 | 111.2±14.0 |
| Group B | 5.0±2.3 | 76.7±32.9 |
| Group C | 4.3±0.6 | 70.8±16.5 |
| Group D | 3.7±0.6 | 61.2±16.3 |
Note: p < 0.05 in comparison with the blank.
Although the invention has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this disclosure. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure and claims of this application. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (1)
1. The extraction method of the effective components in the prinsepia utilis royle is characterized by comprising the following steps: the method comprises the following steps:
1) crushing 1kg of dried prinsepia utilis royle, putting into a low-temperature squeezer, and squeezing at low temperature of 4 ℃ to obtain prinsepia utilis royle crude oil;
2) centrifuging the crude Prinsepia utilis Royle oil obtained in the step 1) at a high speed of 10000 r/min for 1h by using a refrigerated centrifuge, and allowing supernate to pass through an active carbon column to obtain light yellow Prinsepia utilis Royle oil;
3) freezing the prinsepia utilis royle oil obtained in the step 2) at-12 ℃ for 24 hours to generate layering, wherein the lower layer is macromolecular oily solid matter, the upper layer is micromolecular oil, and filtering after freezing to obtain micromolecular prinsepia utilis royle oil;
4) adding anhydrous sodium sulfate accounting for 10% of the mass of the small molecule prinsepia utilis royle oil in the step 3) into the small molecule prinsepia utilis royle oil to adsorb residual water in the oil, magnetically stirring for 1h, and performing suction filtration to remove the sodium sulfate to obtain 160g of dehydrated small molecule prinsepia utilis royle oil;
5) crushing the prinsepia utilis royle dregs obtained after low-temperature pressing in the step 1), performing hot reflux extraction for 2 times by using an ethanol solution with the volume concentration of 65%, combining the extracting solutions, and recovering ethanol to obtain an prinsepia utilis royle ethanol crude extract;
6) adding ethanol into the ethanol crude extract of the Prinsepia utilis Royle in the step 5), stirring and dissolving, wherein the ethanol content in the solution is 40%, filtering insoluble substances, adsorbing total alkaloids in the filtrate by an acrylic acid weak acid cation resin 151 column, washing the column by water after complete adsorption until effluent is colorless, then eluting the total alkaloids by an ethanol solution containing ammonia water, identifying by bismuth potassium iodide, collecting eluent containing the total alkaloids of the Prinsepia utilis Royle to obtain a total alkaloids solution of the Prinsepia utilis Royle, adjusting the pH value of the total alkaloids solution of the Prinsepia utilis Royle to be neutral by hydrochloric acid, concentrating and recovering ethanol to obtain a total alkaloids extract of the Prinsepia utilis Royle, drying the extract, thermally dissolving by absolute ethanol, standing at 0 ℃ for 8 hours, filtering out insoluble ammonium chloride, recovering ethanol from the filtrate; wherein the ethanol solution containing ammonia water is prepared by adding ammonia water into 75% ethanol solution by mass concentration, and the mass percentage concentration of the ammonia water in the solution is 8%;
7) the total alkaloids and dehydrated micromolecular prinsepia utilis royle oil are effective components in the prinsepia utilis royle.
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