KR20160149748A - Method for producing higher-pyrity and depolymerizing fucoidan extracted from brown algae - Google Patents
Method for producing higher-pyrity and depolymerizing fucoidan extracted from brown algae Download PDFInfo
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- KR20160149748A KR20160149748A KR1020150087326A KR20150087326A KR20160149748A KR 20160149748 A KR20160149748 A KR 20160149748A KR 1020150087326 A KR1020150087326 A KR 1020150087326A KR 20150087326 A KR20150087326 A KR 20150087326A KR 20160149748 A KR20160149748 A KR 20160149748A
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- fucoidan
- extract
- molecular weight
- brown algae
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/202—Algae extracts
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/50—Polysaccharides, gums
- A23V2250/51—Polysaccharide
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2300/00—Processes
- A23V2300/14—Extraction
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
Description
The present invention relates to a high purity and low molecular weight fucoidan extracted from brown algae, and more particularly, to a method for extracting fucoidan-containing extract from brown algae and high purity and low molecular weight fucoidan from the extract.
Biomolecules of various natural state before processing exist as macromolecules with molecular weight of several hundred kDa or more, and these macromolecules are digested and absorbed by various enzymes in the body by being low molecular weight. Therefore, a variety of studies have been carried out both domestically and externally with respect to various methods for lowering the molecular weight of a molecule having a relatively small molecular weight in order to facilitate the absorption of these polymers into the body.
On the other hand, seaweeds consist of 60 ~ 95% of water, and carbohydrates are the most among the remaining components, so that the amount of carbohydrate is almost 50% or more based on the total amount. There are a lot of ash after the carbohydrate, and 40% depending on the kind. Polysaccharide, which is a major component of seaweed, can be divided into three types: cell wall polysaccharide which is a fine fibrous crystal structure in the outer layer, viscous polysaccharide in an amorphous gel state covering it, and storage polysaccharide in a cell.
Seaweeds are largely classified as green algae, brown algae, and red algae, and their constituents vary slightly depending on the species. The usefulness of these polysaccharides is as follows. 2) anti-cholesterol effect (seaweed acid polysaccharide); and 3) anti-cancer effect (sulfuric acid polysaccharide of cuttlefish, sulfuric acid polysaccharide, Nutritional effect (soluble fiber), energy resources (biomass), seaweed biotechnology (manufactured by protozoa using heododecase), and so on.
The polysaccharides are composed of 1) cell wall polysaccharide (straight chain glucan of β-D-Glc- (1 → 4)), 2) viscous polysaccharide (seaweed specific polysaccharide, alginic acid, fucoidan, sargassan), 3) storage polysaccharides (lamina). Especially, alginic acid is used for Na-alginate food additive, fiber (rayon icicle), ice cream, cheese stabilizer, medical gauze, diet foods (Sr, Cd absorption suppression, dressing action, calories).
It is known that the brown algae contain calcium, potassium, magnesium, iodine, iron and zinc which are known as the fifth nutrients and have excellent efficacy for the growth of human and animal and prevention of various diseases have.
Seaweed contains various useful substances, but it is particularly noteworthy that fucoidan and inorganic components are the ingredients of the present invention. The ingredient to be utilized by the applicant of the present invention is fucoidan and its content and efficacy are as follows.
Fucoidan contained in seaweeds has an average molecular weight of 20KDa and has a basic sugar called sulfate (Fucose) and a sulfate group. There are two kinds of U-fucoidan containing F-fucoidan and 20% of glucuronic acid from sulfated fucoidan.
It is known as the basic raw material, dietary supplement, additive, and special drug of functional food. Fucoidan imparts flexibility to brown algae and protects them from the flow of intense tide. Fucoidan helps cholesterol excretion, lowers blood cholesterol levels, prevents vascular disease, and prevents obesity from leading to adult disease. In Japan and the United States, fucoidan has been shown to inhibit blood clotting, antitumor action, gastric ulcer treatment, antimicrobial action, inhibition of hypertension, production of hepatocyte growth factor (HGF), inhibition of hyperglycemia, It is known to have antiviral action. Fucoidan has been reported to have a 70-80% effect on the healing of cancers of the gastro-intestinal system, and it has excellent effects on most cancer healing. F-fucoidan induces suicide of lymphoid stem cells, Of the patients.
In particular, fucoidan is a polysaccharide containing a high proportion of sulfated polysaccharides and is generally a macromolecule with a molecular weight of more than 300 kDa, derived from some marine invertebrates such as seaweed, sea cucumbers and sea urchins, such as kelp. Such fucoidan has been reported to have various biological activities such as anti-inflammation, anti-cancer, immunomodulation, anticoagulation, anti-thrombosis, and antioxidant. However, since our body does not decompose fucoidan into its own and can not make it low-molecular, it is excreted without absorbing most of it even though it consumes a large quantity of fucoidan as it is.
Despite the numerous advantages of fucoidan exhibiting various biological activities as described above, the fucoidan extracted from brown algae has a molecular weight of 20 KDa as well as polysaccharides including alginic acid and is a polymer substance having a molecular weight of 1,000 KDa or more. It is desirable to take a low-molecular-weight product for the absorption of fucoidan, and thus it is required to develop a method for producing a low molecular weight fucoidan.
Disclosure of the Invention The present invention is conceived to solve the above-described problems, and it is an object of the present invention to provide a method for producing fucoidan by extracting fucoidan from brown algae, which is an inexpensive raw material, to make high purity and low molecular weight.
However, the objects of the present invention are not limited to the above-mentioned objects, and other objects not mentioned can be clearly understood by those skilled in the art from the following description.
The present invention relates to a pretreatment step of drying and desalting brown algae; An extraction step of extracting fucoidan extract from the pretreated brown algae; A high purity step of precipitating and removing alginic acid from the extracted fucoidan extract; A low molecular weight step of lowering the fucoidan by adding an acid containing a carboxy group to the highly purified fucoidan extract; And a purification step of removing other foreign substances from the low molecular weight fucoidan extract.
Also, the pretreatment step according to the present invention is a step of desalting salts by eluting the salts in the fresh water continuously flowing after natural drying of the brown algae.
Further, the extracting step according to the present invention is a step of extracting for 30 to 60 minutes under a steam of 120 to 140 ° C.
In addition, the highly purified step according to the present invention is a step of stirring by the addition of 2 to 4 parts by weight of CaCl 2 aqueous solution with respect to the post-heated by the extracted fucoidan extract of 30 to 50 ℃, 100 parts by weight of the total extract.
In addition, the low-molecular-weight fraction according to the present invention may be prepared by heating the highly purified fucoidan extract to 80 to 90 ° C, adding 5 to 10 parts by weight of an organic acid having a carboxy group to 100 parts by weight of the total extract, .
In addition, the low-molecular-weight compound according to the present invention is prepared by adding acetic acid for food additives containing acetic acid (CH 3 COOH) to an organic acid containing a carboxy group and stirring the mixture.
In addition, the low-molecular-weight compound according to the present invention may further include a step of adding an organic acid containing the carboxy group, stirring the mixture, cooling the mixture to room temperature, and neutralizing the pH of the Fucoidan extract to 5.0 to 5.5.
The purification step according to the present invention is a step of passing the low molecular weight fucoidan extract through a resin filter.
Further, in the purification step according to the present invention, after passing through the resin filter, it is passed through an activated carbon filter to remove other foreign matter and odor.
Further, the present invention further includes a processing step of pulverizing or liquefying the purified Fucoidan extract after the purification step according to the present invention.
According to the fucoidan production method of one embodiment of the present invention, fucoidan components can be extracted from brown algae, and purification yield of fucoidan can be increased through a high purification process and a low molecular weight process. Fucoidan having a molecular weight easily absorbed into the human body Can be provided.
According to the present invention, a fucoidan suitable for use as a food material can be provided by providing a low molecular weight process capable of hydrolyzing fucoidan even under weak acid conditions.
According to the present invention, most of the seaweed calcium and fucoidan raw materials consumed at present are dependent on imports. Therefore, the development of this technology has the effect of replacing a part of the raw materials consumed in the domestic market. As a useful material recovery technology, it can be applied to the development of various application technologies using it later.
FIG. 1 shows a flowchart of a method of manufacturing fucoidan according to an embodiment of the present invention.
FIG. 2 shows a schematic view of a housing filter system of the purification step according to an embodiment of the present invention.
FIG. 3 shows a log MW value according to the retention time of the molecular weight control for measuring the molecular weight of fucoidan prepared by the fucoidan preparation method according to an embodiment of the present invention, and an equation approximating the log MW value.
Before describing the present invention in detail, it is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of the invention, which is defined solely by the appended claims. shall. All technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art unless otherwise stated.
Throughout this specification and claims, the word "comprise", "comprises", "comprising" means including a stated article, step or group of articles, and steps, , Step, or group of objects, or a group of steps.
On the contrary, the various embodiments of the present invention can be combined with any other embodiments as long as there is no clear counterpoint. Any feature that is specifically or advantageously indicated as being advantageous may be combined with any other feature or feature that is indicated as being preferred or advantageous. Hereinafter, embodiments of the present invention and effects thereof will be described with reference to the accompanying drawings.
A method for producing fucoidan according to an embodiment of the present invention is a method for extracting fucoidan from brown algae and purifying and purifying it by high-purity and low molecular weight, comprising a pretreatment step (S10) for drying and desalting brown algae; An extraction step (S20) of extracting fucoidan extract from pretreated brown algae; A high purity step (S30) in which alginic acid is precipitated and removed from the extracted fucoidan extract; A low-molecular-weight step (S40) in which an acid is added to the high-purity fucoidan extract to lower the fucoidan; And a purification step (S50) of removing other foreign substances from the low-molecular-weight fucoidan extract. FIG. 1 shows a flowchart of a method of manufacturing fucoidan according to an embodiment of the present invention.
A pretreatment step (S10) according to an embodiment of the present invention is a step of drying and desalting brown algae, and removing moisture and salts from brown algae to extract fucoidan contained in brown algae with high purity.
The pretreatment step (S10) may be a step of desalting salts by eluting the salts in the fresh water continuously flowing after drying the brown algae naturally.
The brown algae are a kind of seaweed, and include all kinds of fucoidan components, including, but not limited to, seaweed, seaweed, rhubarb, kelp, seaweed, It is preferable to use a toothbrush or a toothbrush. It is more preferable to use the pot collected from the sea coast of Jeju.
Brown algae are dried using solar and natural winds. In the case of clear weather with low amount of clouds, it is preferable to dry for about 1 to 2 days.
The dried brown algae are desalted by using fresh water so that the brown algae can be sufficiently submerged and the fresh water is continuously exchanged to elute the foreign substances on the surface of brown algae and the brown algae for 50 to 70 minutes, give. The desalting process as described above is repeated two to three times to sufficiently remove foreign substances and salts.
The extracting step (S20) according to an embodiment of the present invention is a step of extracting Fucoidan extract from pretreated brown algae and extracting an extract containing fucoidan and other ingredients (hereinafter, referred to as " Fucoidan extract ').
The extraction step S20 may be a step of extracting for 30 to 60 minutes under a steam of 120 to 140 占 폚. For example, the harvested extract is steamed for 30 minutes with steam to obtain an extract containing a steaming liquid, that is, fucoidan. The extracted Fucoidan extract contains not only fucoidan components but also heavy metal components such as polysaccharides such as alginic acid and arsenic.
The high purity step (S30) according to an embodiment of the present invention is a step of precipitating alginic acid from the extracted Fucoidan extract to remove alginic acid contained in 20 to 30% of the extracted Fucoidan extract to obtain high purity Fucoidan extract .
In the high purity step (S30), the extracted Fucoidan extract is heated to 30 to 50 DEG C, and 2 to 4 parts by weight of a calcium salt aqueous solution is added to 100 parts by weight of the total extract, followed by stirring to precipitate and remove alginic acid have.
When the calcium salt aqueous solution is added in an amount of less than 2 parts by weight, the alginic acid is not sufficiently removed and remains in the fucoidan extract, so that the effect of high purity is not significant. When the addition amount exceeds 4 parts by weight, There is a problem.
The calcium salt aqueous solution may be an aqueous solution of calcium chloride (CaCl 2 ), an aqueous solution of calcium carbonate (CaCO 3 ), calcium hydroxide (Ca (OH) 2 ) or the like, preferably an aqueous solution of calcium chloride (CaCl 2 ).
The calcium salt aqueous solution is added at the same time as the extracted Fucoidan extract is stirred. The stirring time is 1 to 2 minutes and further stirring is performed for 1 to 2 minutes.
The collection method of the precipitated alginic acid is not particularly limited, but it may be preferable to collect by centrifugation.
In the case of proceeding to a low-molecular-weight step (S40) to be described later without going through the high-purity step (S30), that is, when acetic acid treatment is carried out without removing alginic acid in the fucoidan extract, the alginate in the extract is flocculated It shows the behavior that gives priority to the action. Therefore, after the alginic acid removal is completed through the high purity step S30, the low molecular weight step S40 is performed.
In the low-molecular-weight step (S40) according to an embodiment of the present invention, acid is added to the highly purified fucoidan extract to lower-molecularize the fucoidan. The fucoidan having an average molecular weight Mw of 20 KDa is subjected to acid hydrolysis, 20,000 low molecular weight fucoidan is obtained.
In the low-molecular-ization step (S40), the highly purified fucoidan extract is heated to 80 to 90 DEG C, and then an organic acid (carboxylic acid) containing 5 to 10 parts by weight of a carboxy group (-COOH) And stirring the mixture.
For the hydrolysis of the polysaccharide in the alginic acid-removed fucoidan extract, a relatively weakly acidic carboxylic acid is used. The carboxylic acid preferably has 20 carbon atoms or less, more preferably 10 carbon atoms or less. For example, acetic acid for food additives containing acetic acid (CH 3 COOH) is preferably used. The present invention uses a weak acid in consideration of the fact that fucoidan is used as a raw material for food, unlike the case of using a strong acid as a conventional fucoidan low molecular weight method.
Since the acid used for hydrolysis is not a strong acid, it is preferable to perform heating in the above temperature range in order to increase hydrolysis efficiency.
When the amount of the carboxylic acid added is less than 5 parts by weight, hydrolysis of the polysaccharide in the extract is not sufficiently performed, resulting in low efficiency of low molecular weight. When the amount is more than 10 parts by weight, the use value of fucoidan as a raw material for fucoidan .
In the low-molecular-ization step (S40), the low-molecular-weight fucoidan extract is cooled to room temperature, and then an aqueous solution of caustic soda (sodium hydroxide, NaOH) of 30 to 50% is added and stirred to neutralize the extract to pH 5.0 to 5.5 can do.
The purifying step (S50) according to an embodiment of the present invention is a step of removing other foreign substances from the low molecular weight fucoidan extract, and it is possible to further increase the purity of fucoidan by lowering the concentration of other sugars excluding heavy metals such as arsenic and fucoidan have.
The purification step (S50) may be a step of passing the low-molecular-weight fucoidan extract through a resin filter. The filter includes a resin capable of filtering a specific component, and purification is possible by passing the extract through a housing filter containing, for example, arsenic removal resin or anion resin.
More specifically, in order to remove heavy metals such as arsenic, the low molecular weight fucoidan extract may be passed through an arsenic removal system composed of three stages using a housing filter including an arsenic removal resin to adsorb and remove arsenic present in the extract. For example, TULSION, an ion exchange resin for removing arsenic ? ARSENIL and the like can be used.
Further, in order to lower the concentration of other saccharides other than fucoidan present in the Fucoidan extract through which the arsenic was removed by passing through the arsenic removal filter, the extract was passed through a purification apparatus using a housing filter containing an anionic resin to obtain a fucoidan extract Can be obtained. As the anion resin, for example, GS300 and the like can be used as the strongly basic anionic resin.
Further, the purification step (S50) may further include the step of passing through the resin filter and passing through the activated carbon filter to remove other foreign matter and algae odor.
The activated carbon filter can be used without limitation as long as it is an activated carbon filter capable of adsorbing odor and other foreign matter. FIG. 2 shows a schematic view of a housing filter system of the purification step according to an embodiment of the present invention.
The method for preparing fucoidan according to an embodiment of the present invention further comprises a processing step (S60) of pulverizing or liquefying the purified fucoidan extract after the purification step (S50).
Examples and Comparative Examples
Example 1
Using natural sunlight and natural winds, it was dried until the surface turned white (it can be dried for 1 ~ 2 days depending on the weather). And the fresh water was continuously exchanged, and the foreign matter and saline were eluted in about 1 hour, and then taken out and hanged in the air, the water was sufficiently removed to the extent that the water did not fall into the natural drop, Pretreatment by removing salt and foreign matter.
The pretreated extracts were fucoidan extracts containing fucoidan for 30 minutes using steam at 140 ℃.
The fucoidan extracts extracted from roots were warmed to 40 ° C, and 4 g of 50% CaCl 2 aqueous solution prepared by dissolving CaCl 2 in fresh water was added to each 100 g of Fucoidan extract. The resulting alginic acid precipitate was centrifuged at 10,000 rpm Respectively.
The fucoidan extract from which the alginate was removed was heated to 90 ° C., and 5 g of acetic acid was added per 100 g of the extract. The resulting solution was stirred for about 1 hour to acid-hydrolyze the polymeric fucoidan with a low-molecular fucoidan. After the hydrolysis, 40% aqueous solution of caustic soda was neutralized by adding it until the pH reached 5.5.
The low molecular weight fucoidan extract is passed through a housing filter including an arsenic removal resin, a filter including anion resin for removing other saccharides, and an activated carbon filter in order to adsorb and remove arsenic present in the extract, remove other saccharides other than fucoidan , And the other foreign substances contained in the extract and seaweed flesh were removed to obtain high purity low molecular weight fucoidan.
Examples 2 and 3
Fucoidan was prepared in the same manner as in Example 1 except that the fucoidan was changed as shown in Table 1 below.
Comparative Example
Fucoidan was prepared in the same manner as in Example 1 except that the fucoidan was changed as shown in Table 1 below.
(° C)
(min)
(° C)
Experimental Example
(1) Average molecular weight measurement
The average molecular weight of fucoidan prepared in the above Example was measured using HPLC-R1. FIG. 3 shows a log Mw value according to the retention time of the molecular weight control for the molecular weight measurement and an equation approximating the log Mw value. The molecular weight measurement results according to the retention time are shown in Table 2.
(2) Analysis of sulfate group content
Sulfate groups were measured by BaCl 2 / gelation method as follows.
When 400 ml of distilled water was heated to 60-70 ° C, 2 g of gelatin was added and stirred. To the solution stored in the refrigerator, 2 g of BaCl 2 was added, stirred, and stored in a refrigerator (4 ° C) for 3 hours to prepare a BaCl 2 -gelatin reagent. 1 ml of test specimen was mixed with 0.5 ml of 2 mg / ml specimen and 0.5 ml of 4M HCl in a micro tube and reacted at 105 ° C for 4-5 hours in a dry oven. The absorbance at 360 nm was measured using a spectrophotometer. Table 3 shows the content of sulfuric acid groups measured by substituting the standard curve obtained using the standard material.
(3) Fucose content analysis
Fucoidan contents were analyzed to determine the degree of high purity. The measurement was performed by HPAEC (High Performance Anion Electric Chromatography) and the detector was analyzed by PAD (Pulsed Amperometry Detector).
The features, structures, effects, and the like illustrated in the above-described embodiments can be combined and modified in other embodiments by those skilled in the art to which the embodiments belong. Therefore, it should be understood that the present invention is not limited to these combinations and modifications.
Claims (10)
An extraction step of extracting fucoidan extract from the pretreated brown algae;
A high purity step of precipitating and removing alginic acid from the extracted fucoidan extract;
A low molecular weight step of lowering the fucoidan by adding an acid containing a carboxy group to the highly purified fucoidan extract; And
And removing the foreign substance from the low molecular weight fucoidan extract.
Wherein the pretreatment step is a step of desalting salt by eluting salts in the fresh water continuously flowing after natural drying of the brown algae.
Wherein said extracting step is a step of extracting under steam at 120 to 140 DEG C for 30 to 60 minutes.
Wherein the high purity step is a step of heating the extracted Fucoidan extract to 30 to 50 캜 and then adding 2 to 4 parts by weight of CaCl 2 aqueous solution to 100 parts by weight of the whole extract and stirring.
Wherein the high-purity fucoidan extract is heated to 80 to 90 ° C, and 5 to 10 parts by weight of an organic acid containing a carboxy group is added to 100 parts by weight of the total extract, followed by stirring.
Wherein the low molecular weight is a step of adding acetic acid for food additives containing acetic acid (CH 3 COOH) as an organic acid containing the carboxy group and stirring the mixture.
Wherein the low molecular weight step further comprises a step of adding an organic acid containing the carboxy group and stirring the mixture, then cooling the mixture to room temperature, and then neutralizing the pH of the Fucoidan extract to 5.0 to 5.5.
Wherein the purification step comprises passing the low molecular weight fucoidan extract through a resin filter.
Wherein the purifying step is a step of passing through the resin filter and passing through an activated carbon filter to remove other foreign matter and odor.
And a processing step of pulverizing or liquefying the purified fucoidan extract after the purification step.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018199503A1 (en) * | 2017-04-26 | 2018-11-01 | 씨제이제일제당(주) | Nipa palm extract preparation method and nipa palm extract prepared by means of same |
| KR20190092146A (en) * | 2018-01-30 | 2019-08-07 | (주)한국바이오엔지니어링 | Alginic acid propylene glycol ester production method containing fucoxanthin and fucoidan with reduced off-flavor |
| WO2022139115A1 (en) * | 2020-12-22 | 2022-06-30 | (주)해림후코이단 | Method for preparation of alginic acid and fucoidan |
| KR20220135687A (en) * | 2021-03-31 | 2022-10-07 | 엠에스글로벌바이오주식회사 | Manufacturing method for cosmetic composition of fucoidan seaweed and functional and anti-aging cosmetic composition thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018199503A1 (en) * | 2017-04-26 | 2018-11-01 | 씨제이제일제당(주) | Nipa palm extract preparation method and nipa palm extract prepared by means of same |
| US11318397B2 (en) | 2017-04-26 | 2022-05-03 | Cj Cheiljedang Corporation | Nipa palm extract preparation method and nipa palm extract prepared by means of same |
| KR20190092146A (en) * | 2018-01-30 | 2019-08-07 | (주)한국바이오엔지니어링 | Alginic acid propylene glycol ester production method containing fucoxanthin and fucoidan with reduced off-flavor |
| WO2022139115A1 (en) * | 2020-12-22 | 2022-06-30 | (주)해림후코이단 | Method for preparation of alginic acid and fucoidan |
| KR20220135687A (en) * | 2021-03-31 | 2022-10-07 | 엠에스글로벌바이오주식회사 | Manufacturing method for cosmetic composition of fucoidan seaweed and functional and anti-aging cosmetic composition thereof |
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