CN109355202A - A kind of Cordceps militaris cultural method - Google Patents
A kind of Cordceps militaris cultural method Download PDFInfo
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- CN109355202A CN109355202A CN201811399117.6A CN201811399117A CN109355202A CN 109355202 A CN109355202 A CN 109355202A CN 201811399117 A CN201811399117 A CN 201811399117A CN 109355202 A CN109355202 A CN 109355202A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The present invention relates to the technical fields of cordyceps sinensis culture to prevent the effect of spawn degeneration and multiple shape more particularly to a kind of Cordceps militaris cultural method, and generation mycelium pellet is small, is easy to spray inoculation；Yield is high and quality is good；The following steps are included: 1), prepare Cordceps militaris liquid spawn culture medium formula: it includes following raw material, freshly-slaughtered poultry brisket, glucose and water, wherein above-mentioned raw-material weight are as follows: 1 gram of freshly-slaughtered poultry brisket, 0.2-0.5 grams of glucose and 40-80 grams of water；2) Cordceps militaris liquid spawn culture medium, is configured；3), prepare the formula of cordyceps culturing medium: it includes following raw material, dry wheat, freshly-slaughtered poultry brisket, jujube and water, wherein above-mentioned raw-material weight are as follows: 30-35 grams of dry wheat, 0.5-1 grams of freshly-slaughtered poultry brisket, 0.5-1 grams of jujube and 60-70 grams of water；4) cordyceps culturing medium, is configured.
Description
Technical field
The present invention relates to the technical fields of cordyceps sinensis culture, more particularly to a kind of Cordceps militaris cultural method.
Background technique
It is well known that Cordceps militaris is the abbreviation of northern Chinese caterpillar Fungus, it is also Cordyceps militaris or pupa grass, is the medicinal true of bacterium combination
Bacterium.It is exactly cordyceps sinensis that many people, which take for Cordceps militaris, and actually this is not so, and the two has the difference of essence, and cordyceps sinensis is extremely
Rare wild Chinese medicine；Existing Cordceps militaris cultural method low output, poor quality, and it is not easy to spray inoculation.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of effect for preventing spawn degeneration and multiple shape, mycelium pellet is generated
It is small, it is easy to spray inoculation；The Cordceps militaris cultural method that yield is high and quality is good.
A kind of Cordceps militaris cultural method of the invention, comprising the following steps:
1), prepare the formula of Cordceps militaris liquid spawn culture medium: it includes following raw material, freshly-slaughtered poultry brisket, glucose and
Water, wherein above-mentioned raw-material weight are as follows: 1 gram of freshly-slaughtered poultry brisket, 0.2-0.5 grams of glucose and 40-80 grams of water；
2) Cordceps militaris liquid spawn culture medium, is configured；
2-1), remove the corner fat of freshly-slaughtered poultry brisket；
2-2), a small amount of boiling is added to boil to 100 DEG C, duration 5 minutes；
2-3), the liquid produced in step 2 is taken to be filtered；
2-4), it takes filtrate and glucose is added；
2-5), it is several to prepare 1000ml capacity triangular flask, and the filtrate generated in step (2-4) is respectively put into each three
In the bottle of angle, 400-500ml is placed in each triangular flask, tampon is clogged into bottleneck, be put into high-pressure sterilizing pot and sterilize, 121 DEG C of temperature,
It time 50~60 minutes, is cooled to room temperature, 22 DEG C or less；
3), prepare the formula of cordyceps culturing medium: it includes following raw material, dry wheat, freshly-slaughtered poultry brisket, jujube and water,
Wherein above-mentioned raw-material weight are as follows: 30-35 grams of dry wheat, 0.5-1 grams of freshly-slaughtered poultry brisket, 0.5-1 grams of jujube and 60-70 grams of water；
4) cordyceps culturing medium, is configured；
4-1), selection current year without the dry wheat of mildew and goes native impurity elimination；
4-2), freshly-slaughtered poultry brisket is boiled 5 minutes, is smashed；
4-3), jujube impregnates 24 hours, and water is added to smash；
4-4), the Fresh Grade Breast after smashing, jujube and water are mixed in proportion；It pours into vial；
4-5), dry wheat is poured into proportion in vial, is sealed with polypropylene screen, high pressure sterilization 60 minutes, is cooled to
Room temperature, 22 DEG C or less；
5) strain cultivation liquid triangular flask after cooling, is put into superclean bench, sterile working is carried out and accesses 1-1.5
Square centimeter Cordceps militaris solid parent species, bottleneck are stoppered tampon, are placed on oscillator at once and vibrate, and 55~70 times per minute, air
It 18~20 DEG C of temperature, vibrates 80~120 hours, relative air humidity 50% is hereinafter, light is placed on 20 DEG C of trainings less than 50xl
It supports；
6) Cordceps militaris liquid spawn, inoculating gun and bottled cordyceps culturing medium that step 5) obtains, are put into ultra-clean work
Make platform, liquid spawn bottom is protruded into inoculating gun one end, is stoppered continuous plug, carries out sterile working, liquid spawn is uniformly sprayed into bottled
Cordyceps culturing medium surface, then sealed bottleneck with polypropylene screen；
7) inoculated culture medium is placed on light less than 50xl by, Mycelium culture management, and 20-22 DEG C of temperature, air
Relative humidity less than 60%, the time be 10-15 days, 200-300 DEG C of accumulated temperature, until mycelia covers with bottle；
8), go out grass to manage, 1, annesl, light 100-300xl, 18-21 DEG C of temperature, relative air humidity 80-85%, the time
It is 4-7 days, forms former base；
9) it, growing, light 100-300XL, 18-21 DEG C of temperature, relative air humidity 80-85%, the time is 20-30 days,
10), mature, light 100-200XL, temperature is down to 16-20 DEG C, relative air humidity 80-85%, the time 10
It, finished product harvests out.
Compared with prior art the invention has the benefit that preventing the effect of spawn degeneration and multiple shape, mycelium pellet is generated
It is small, it is easy to spray inoculation；Yield is high, and quality is good.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not intended to limit the scope of the invention.
A kind of Cordceps militaris cultural method of the invention, comprising the following steps:
1), prepare the formula of Cordceps militaris liquid spawn culture medium: it includes following raw material, freshly-slaughtered poultry brisket, glucose and
Water, wherein above-mentioned raw-material weight are as follows: 1 gram of freshly-slaughtered poultry brisket, 0.2-0.5 grams of glucose and 40-80 grams of water；
2) Cordceps militaris liquid spawn culture medium, is configured；
2-1), remove the corner fat of freshly-slaughtered poultry brisket；
2-2), a small amount of boiling is added to boil to 100 DEG C, duration 5 minutes；
2-3), the liquid produced in step 2 is taken to be filtered；
2-4), it takes filtrate and glucose is added；
2-5), it is several to prepare 1000ml capacity triangular flask, and the filtrate generated in step (2-4) is respectively put into each three
In the bottle of angle, 400-500ml is placed in each triangular flask, tampon is clogged into bottleneck, be put into high-pressure sterilizing pot and sterilize, 121 DEG C of temperature,
It time 50~60 minutes, is cooled to room temperature, 22 DEG C or less；
3), prepare the formula of cordyceps culturing medium: it includes following raw material, dry wheat, freshly-slaughtered poultry brisket, jujube and water,
Wherein above-mentioned raw-material weight are as follows: 30-35 grams of dry wheat, 0.5-1 grams of freshly-slaughtered poultry brisket, 0.5-1 grams of jujube and 60-70 grams of water；
4) cordyceps culturing medium, is configured；
4-1), selection current year without the dry wheat of mildew and goes native impurity elimination；
4-2), freshly-slaughtered poultry brisket is boiled 5 minutes, is smashed；
4-3), jujube impregnates 24 hours, and water is added to smash；
4-4), the Fresh Grade Breast after smashing, jujube and water are mixed in proportion；It pours into vial；
4-5), dry wheat is poured into proportion in vial, is sealed with polypropylene screen, high pressure sterilization 60 minutes, is cooled to
Room temperature, 22 DEG C or less；
5) strain cultivation liquid triangular flask after cooling, is put into superclean bench, sterile working is carried out and accesses 1-1.5
Square centimeter Cordceps militaris solid parent species, bottleneck are stoppered tampon, are placed on oscillator at once and vibrate, and 55~70 times per minute, air
It 18~20 DEG C of temperature, vibrates 80~120 hours, relative air humidity 50% is hereinafter, light is placed on 20 DEG C of trainings less than 50xl
It supports；
6) Cordceps militaris liquid spawn, inoculating gun and bottled cordyceps culturing medium that step 5) obtains, are put into ultra-clean work
Make platform, liquid spawn bottom is protruded into inoculating gun one end, is stoppered continuous plug, carries out sterile working, liquid spawn is uniformly sprayed into bottled
Cordyceps culturing medium surface, then sealed bottleneck with polypropylene screen；
7) inoculated culture medium is placed on light less than 50xl by, Mycelium culture management, and 20-22 DEG C of temperature, air
Relative humidity less than 60%, the time be 10-15 days, 200-300 DEG C of accumulated temperature, until mycelia covers with bottle；
8), go out grass to manage, 1, annesl, light 100-300xl, 18-21 DEG C of temperature, relative air humidity 80-85%, the time
It is 4-7 days, forms former base；
9) it, growing, light 100-300XL, 18-21 DEG C of temperature, relative air humidity 80-85%, the time is 20-30 days,
10), mature, light 100-200XL, temperature is down to 16-20 DEG C, relative air humidity 80-85%, the time 10
It, finished product harvests out.
The effect of spawn degeneration and multiple shape is prevented, generation mycelium pellet is small, is easy to spray inoculation；Yield is high, and quality is good.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvements and modifications, these improvements and modifications can also be made
Also it should be regarded as protection scope of the present invention.
Claims (1)
1. a kind of Cordceps militaris cultural method, which comprises the following steps:
1), prepare the formula of Cordceps militaris liquid spawn culture medium: it includes following raw material, freshly-slaughtered poultry brisket, glucose and water,
In above-mentioned raw-material weight are as follows: 1 gram of freshly-slaughtered poultry brisket, 0.2-0.5 grams of glucose and 40-80 grams of water；
2) Cordceps militaris liquid spawn culture medium, is configured；
2-1), remove the corner fat of freshly-slaughtered poultry brisket；
2-2), a small amount of boiling is added to boil to 100 DEG C, duration 5 minutes；
2-3), the liquid produced in step 2 is taken to be filtered；
2-4), it takes filtrate and glucose is added；
2-5), it is several to prepare 1000ml capacity triangular flask, and the filtrate generated in step (2-4) is respectively put into each triangular flask
It is interior, 400-500ml is placed in each triangular flask, tampon is clogged into bottleneck, is put into high-pressure sterilizing pot and is sterilized, 121 DEG C of temperature, the time
It 50~60 minutes, is cooled to room temperature, 22 DEG C or less；
3), prepare the formula of cordyceps culturing medium: it includes following raw material, dry wheat, freshly-slaughtered poultry brisket, jujube and water, wherein
Above-mentioned raw-material weight are as follows: 30-35 grams of dry wheat, 0.5-1 grams of freshly-slaughtered poultry brisket, 0.5-1 grams of jujube and 60-70 grams of water；
4) cordyceps culturing medium, is configured；
4-1), selection current year without the dry wheat of mildew and goes native impurity elimination；
4-2), freshly-slaughtered poultry brisket is boiled 5 minutes, is smashed；
4-3), jujube impregnates 24 hours, and water is added to smash；
4-4), the Fresh Grade Breast after smashing, jujube and water are mixed in proportion；It pours into vial；
4-5), dry wheat is poured into proportion in vial, is sealed with polypropylene screen, high pressure sterilization 60 minutes, is cooled to room temperature,
22 DEG C or less；
5) strain cultivation liquid triangular flask after cooling, is put into superclean bench, sterile working is carried out and accesses 1-1.5 squares
Centimetre Cordceps militaris solid parent species, bottleneck are stoppered tampon, are placed on oscillator oscillation at once, and 55~70 times per minute, air themperature
It 18~20 DEG C, vibrates 80~120 hours, relative air humidity 50% is hereinafter, light is placed on 20 DEG C of cultures less than 50xl；
6) Cordceps militaris liquid spawn, inoculating gun and bottled cordyceps culturing medium that step 5) obtains, are put into superclean bench,
Liquid spawn bottom is protruded into inoculating gun one end, is stoppered continuous plug, carries out sterile working, liquid spawn is uniformly sprayed into bottled Cordceps militaris
Media surface, then sealed bottleneck with polypropylene screen；
7) inoculated culture medium is placed on light less than 50xl by, Mycelium culture management, and 20-22 DEG C of temperature, air is opposite
Humidity less than 60%, the time be 10-15 days, 200-300 DEG C of accumulated temperature, until mycelia covers with bottle；
8), go out grass to manage, 1, annesl, light 100-300xl, 18-21 DEG C of temperature, relative air humidity 80-85%, time 4-
7 days, form former base；
9) it, growing, light 100-300XL, 18-21 DEG C of temperature, relative air humidity 80-85%, the time is 20-30 days,
10), mature, light 100-200XL, temperature is down to 16-20 DEG C, relative air humidity 80-85%, and the time is 10 days, out
Finished product harvesting.
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| CN201811399117.6A CN109355202A (en) | 2018-11-22 | 2018-11-22 | A kind of Cordceps militaris cultural method |
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| CN101173221A (en) * | 2007-11-09 | 2008-05-07 | 乔善成 | Wheat cultivation Cordyceps militaris method |
| CN101463326A (en) * | 2008-12-30 | 2009-06-24 | 广东省微生物研究所 | Production method of Guangdong Cordyceps sinensismycelium |
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2018
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| CN105274006A (en) * | 2015-07-08 | 2016-01-27 | 大连春天生物技术有限公司 | Medium for cultivating Cordyceps and preparation method thereof |
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