CN110074403A - A kind of powder of edible fungus and preparation method thereof - Google Patents
A kind of powder of edible fungus and preparation method thereof Download PDFInfo
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- CN110074403A CN110074403A CN201910390357.8A CN201910390357A CN110074403A CN 110074403 A CN110074403 A CN 110074403A CN 201910390357 A CN201910390357 A CN 201910390357A CN 110074403 A CN110074403 A CN 110074403A
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- 239000000843 powder Substances 0.000 title claims abstract description 54
- 241000233866 Fungi Species 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 35
- 239000001963 growth medium Substances 0.000 claims abstract description 65
- 238000009630 liquid culture Methods 0.000 claims abstract description 47
- 239000002609 medium Substances 0.000 claims abstract description 43
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 41
- 239000007787 solid Substances 0.000 claims abstract description 39
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 35
- 241000894007 species Species 0.000 claims abstract description 33
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 33
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 24
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 24
- 235000000346 sugar Nutrition 0.000 claims abstract description 22
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 21
- 239000008103 glucose Substances 0.000 claims abstract description 19
- 244000068988 Glycine max Species 0.000 claims abstract description 16
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 16
- 241000209140 Triticum Species 0.000 claims abstract description 16
- 235000021307 Triticum Nutrition 0.000 claims abstract description 16
- 240000008042 Zea mays Species 0.000 claims abstract description 16
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims abstract description 16
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 16
- 235000005822 corn Nutrition 0.000 claims abstract description 16
- 210000000582 semen Anatomy 0.000 claims abstract description 16
- 238000012545 processing Methods 0.000 claims abstract description 3
- 240000008866 Ziziphus nummularia Species 0.000 claims abstract 3
- 230000001954 sterilising effect Effects 0.000 claims description 48
- 239000007788 liquid Substances 0.000 claims description 28
- 241000723418 Carya Species 0.000 claims description 20
- 238000000855 fermentation Methods 0.000 claims description 19
- 230000004151 fermentation Effects 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- 239000008157 edible vegetable oil Substances 0.000 claims description 6
- 241000121220 Tricholoma matsutake Species 0.000 claims description 5
- 241000609666 Tuber aestivum Species 0.000 claims description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 12
- 230000035764 nutrition Effects 0.000 abstract description 12
- 235000013339 cereals Nutrition 0.000 abstract description 11
- 230000036039 immunity Effects 0.000 abstract description 10
- 230000000050 nutritive effect Effects 0.000 abstract description 9
- 239000004615 ingredient Substances 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 235000001727 glucose Nutrition 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 description 32
- 241001247821 Ziziphus Species 0.000 description 13
- 238000001816 cooling Methods 0.000 description 11
- 238000011081 inoculation Methods 0.000 description 10
- 239000004744 fabric Substances 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- 238000001035 drying Methods 0.000 description 6
- 239000000853 adhesive Substances 0.000 description 5
- 230000001070 adhesive effect Effects 0.000 description 5
- 238000009835 boiling Methods 0.000 description 5
- 239000003292 glue Substances 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000011505 plaster Substances 0.000 description 5
- 235000009754 Vitis X bourquina Nutrition 0.000 description 4
- 235000012333 Vitis X labruscana Nutrition 0.000 description 4
- 240000006365 Vitis vinifera Species 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- 238000004140 cleaning Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 238000007664 blowing Methods 0.000 description 3
- 238000010298 pulverizing process Methods 0.000 description 3
- 235000021336 beef liver Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003716 rejuvenation Effects 0.000 description 2
- 208000003643 Callosities Diseases 0.000 description 1
- 208000000059 Dyspnea Diseases 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 240000002769 Morchella esculenta Species 0.000 description 1
- 235000002779 Morchella esculenta Nutrition 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 210000004720 cerebrum Anatomy 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 208000013220 shortness of breath Diseases 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/40—Cultivation of spawn
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention belongs to food processing technology fields, more particularly, to a kind of powder of edible fungus and preparation method thereof.The preparation method of powder of edible fungus in the present invention, the following steps are included: the mycelium for the edible mushroom that liquid culture medium culture parent species are obtained is inoculated into progress bifurcation fermented and cultured in solid medium together with the liquid culture medium together, then dry, crushing, obtains powder of edible fungus；The liquid culture medium ingredient includes potato, wheat bran, glucose and water；The ingredient of the solid medium includes semen sojae atricolor, corn, jujube, wheat, soya bean part and sugar.The powder of edible fungus to be fermented by bifurcation combines the nutrition of five cereals with the nutrition of powder of edible fungus, has higher nutritive value, body immunity can be improved, to build up health.
Description
Technical field
The present invention relates to food processing technology fields, more particularly, to a kind of powder of edible fungus and preparation method thereof.
Background technique
The edible mushrooms such as hickory chick, bolete, matsutake, truffle nutritive value with higher, can with kidney tonifying, establishing-Yang, cerebrum tonifying,
Refresh oneself equivalent, not only full of nutrition, delicious flavour, but also beneficial stomach, indigestion can be treated, the illnesss such as abundant expectoration shortness of breath have
Unique health-care effect.
But the fructification of the edible mushrooms such as hickory chick, bolete is expensive, and the rare wild mushroom such as matsutake, truffle is also
Artificial cultivation is not broken through, can not cultivate fructification.But its mycelium can but be obtained by edible fungus species isolation technics.
Also, the mycelia of edible mushroom has and the comparable nutritive effect of fructification and edible value, for the health of the mankind, improves human body
Immunity, aspect of building up health possess good effect.However, the edible mushroom mycelium of hickory chick and beef liver is also difficult to obtain,
It is caused by people eat and utilize limitation.
In consideration of it, the present invention is specifically proposed.
Summary of the invention
It is an object of the present invention to provide a kind of preparation methods of powder of edible fungus, and liquid culture medium culture parent species are obtained
The mycelium of edible mushroom be inoculated into solid medium progress bifurcation fermented and cultured together, so together with the liquid culture medium
Dry afterwards, crushing, obtains powder of edible fungus；Liquid culture medium ingredient includes potato, wheat bran, glucose and water；Solid medium
Ingredient includes semen sojae atricolor, corn, jujube, wheat, soya bean part and sugar.This method is easy to operate, can carry out industrialization production, save at
This.It is fermented by bifurcation and combines the nutrition of five cereals with the nutrition of powder of edible fungus, obtained powder of edible fungus has and food
The nutritive value similar in mushroom entity can make body immunity increase, to enhance the constitution of people.
It is a further object to provide the powder of edible fungus that the preparation method of powder of edible fungus as described above obtains.Its
Nutritive value with higher, improves the immunity of the human body.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of preparation method of powder of edible fungus, comprising the following steps:
The mycelium for the edible mushroom that liquid culture medium culture parent species obtain is inoculated with together together with the liquid culture medium
Bifurcation fermented and cultured is carried out into solid medium, then dry, crushing obtains powder of edible fungus；
The liquid culture medium is prepared by the component for including following parts by weight:
150-250 parts of potato, 160-250 parts of wheat bran, 15-25 parts of glucose and 1600-2500 parts of water；
The solid medium is prepared by the component for including following parts by weight:
20-28 parts of semen sojae atricolor, 20-30 parts of corn, 5-10 parts of jujube, 20-30 parts of wheat, 20-30 parts of soya bean and sugar 0.8-1.5
Part；
The edible mushroom includes at least one of hickory chick, matsutake, bolete and truffle.
Preferably, the liquid culture medium is prepared by the component for including following parts by weight:
180-220 parts of potato, 190-230 parts of wheat bran, 18-22 parts of glucose and 1800-2200 parts of water.
Preferably, the solid medium is prepared by the component for including following parts by weight:
1-1.3 parts of 24-26 parts of semen sojae atricolor, 23-27 parts of corn, 7-9 parts of jujube, 22-26 parts of wheat, 23-27 parts of soya bean and sugar.
Preferably, the mycelial process for the edible mushroom that the liquid culture medium culture parent species obtain, specifically includes following
Step: taking the thallus tissue cultures of edible mushroom to obtain parent species, will cultivate, is eaten in the liquid culture medium after parent species access sterilizing
With the mycelium of bacterium；
Preferably, the thallus group of the edible mushroom, which is woven in PDA culture medium, cultivates, and obtains parent species；
Preferably, the cultivation temperature in the PDA culture medium is 15-20 DEG C.
Preferably, the liquid culture medium passes through sterilization treatment；
Preferably, the pressure of the sterilizing is 0.14-0.16MPa, and temperature is 118-121 DEG C, time 40-50min；
Preferably, the parent species carry out shaken cultivation in shaking flask；
It is highly preferred that the temperature of the shaken cultivation is 20-23 DEG C, the time of culture is 5-7 days.
Preferably, it after the mycelium for obtaining edible mushroom, is inoculated into before solid medium, further includes by the mycelia
Body is linked into the step of being cultivated in the fermentor of sterilizing；
Preferably, the pressure of the sterilizing of the fermentor is 0.11-0.13MPa, and the time of sterilizing is 35-45min；
Preferably, the time that the liquid mycelium is cultivated in the fermenter is 3-5 days；
Preferably, the liquid culture medium in the fermentor, is prepared by the component of following parts by weight:
90-100 parts of wheat bran water, 0.5-0.8 parts of glucose, 0.4-0.7 parts of white sugar and 0.02-0.05 parts of edible oil.
Limitedly, the humidity of the solid medium is 60%-68%；
Preferably, the sterilising temp of the solid medium is 118-121 DEG C, and sterilization pressure 0.14-0.16MPa goes out
The bacterium time is 2.5-3.5h.
Preferably, the temperature of the bifurcation fermentation is 15-22 DEG C.
Preferably, the time of the bifurcation fermentation is 15-20 days.
The powder of edible fungus that the preparation method of powder of edible fungus as described above obtains.
Compared with the prior art, the invention has the following beneficial effects:
(1) preparation method of the powder of edible fungus in the present invention, the bacterium for the edible mushroom that liquid culture medium culture parent species are obtained
Filament is inoculated into progress bifurcation fermented and cultured in solid medium together with the liquid culture medium together, then drying, crushing,
Obtain powder of edible fungus；Liquid culture medium is prepared by potato, wheat bran, glucose and water, solid medium by semen sojae atricolor, corn,
Jujube, wheat, soya bean part and sugar are prepared.This method is easy to operate, and five cereals are at low cost, cost-saved, can carry out
Industrialization production.The powder of edible fungus to be fermented by bifurcation combines the nutrition of five cereals with the nutrition of powder of edible fungus,
With higher nutritive value, body immunity can be made to increase, to build up health.
(2) the powder of edible fungus nutritive value with higher that the preparation method of heretofore described powder of edible fungus obtains,
Body immunity can be improved.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person carries out according to conventional conditions or manufacturer's recommended conditions.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
A kind of preparation method of powder of edible fungus, comprising the following steps:
The mycelium for the edible mushroom that liquid culture medium culture parent species obtain is inoculated with together together with the liquid culture medium
Bifurcation fermented and cultured is carried out into solid medium, then dry, crushing obtains powder of edible fungus；
The liquid culture medium is prepared by the component for including following parts by weight:
150-250 parts of potato, 160-250 parts of wheat bran, 15-25 parts of glucose and 1600-2500 parts of water；
Liquid culture medium the preparation method comprises the following steps: by potato be added part water, boil 25-35 minutes, with filter screen filtration, wheat bran
It is installed with cloth bag, obtained wheat bran water is added in potato water after boiling 25-35 minutes, glucose is added, boils 20-30 minutes, and
Uniform stirring.Culture solution will be made to pour into shaking flask, bottleneck, inoculation mouth is sealed, sterilizes.Shaking flask is taken after sterilizing
Out, after cooling, parent species are accessed in an aseptic environment.
The solid medium is prepared by the component for including following parts by weight:
20-28 parts of semen sojae atricolor, 20-30 parts of corn, 5-10 parts of jujube, 20-30 parts of wheat, 20-30 parts of soya bean and sugar 0.8-1.5
Part；
By using 20-28 parts of semen sojae atricolors, 20-30 parts of corns, 5-10 portions of jujubes, 20-30 portions of wheats, 20-30 portions of soya beans and
The solid medium that 0.8-1.5 portions of sugar are prepared has nutrition abundant.Sugar is preferably brown sugar.
The solid medium the preparation method comprises the following steps: by semen sojae atricolor, corn, jujube, wheat, soya bean and sugar be mixed, dress
Bacterium bag is made in bag；The specification of the bacterium bag is 15-18cm × 25-28cm.
The edible mushroom includes at least one of hickory chick, matsutake, bolete and truffle.It is preferred that hickory chick or beef liver
Bacterium.
Preferably, the liquid culture medium is prepared by the component of following parts by weight:
180-220 parts of potato, 190-230 parts of wheat bran, 18-22 parts of glucose and 1800-2200 parts of water.
Preferably, the solid medium is prepared by the component of following parts by weight:
1-1.3 parts of 24-26 parts of semen sojae atricolor, 23-27 parts of corn, 7-9 parts of jujube, 22-26 parts of wheat, 23-27 parts of soya bean and sugar；
The present invention leads to the liquid of strain fermentation technique and five cereals for the solid-state of culture medium after the fermentation of the two bifurcation
Drying is crossed, pulverizes and is fabricated to powder of edible fungus.Have and fruit body of edible fungi phase by the powder of edible fungus that this method obtains
Close nutritive value.Body immunity can be improved, build up health, be conducive to the health of human body.
Preferably, the mycelial process for the edible mushroom that the liquid culture medium culture parent species obtain, specifically includes following
Step:
It takes the thallus tissue cultures of edible mushroom to obtain parent species, will cultivate, obtain in the liquid culture medium after parent species access sterilizing
To the liquid mycelium of edible mushroom；
Preferably, the thallus group of the edible mushroom, which is woven in PDA culture medium, cultivates；
The edible mushroom parent species technological process of production includes the following steps: raw material preparation；Culture medium is prepared；Packing sterilizing；It is cooling
Inoculation；Culture and purification and rejuvenation.Tube propagation base is made with traditional PDA, when will grow intact, strong hickory chick picking,
It is with adhesive plaster that its glue is good, miscellaneous bacteria is prevented, cool a 3-5 days under shady and cool environment, by organizing isolated method by hickory chick bacterium
Handle and bacterium emit between tissue remove a fritter under sterile environment and be put into produced culture medium and cultivate.It cultivates and mentions
Pure rejuvenation: the test tube of above-mentioned separator well is cultivated in the environment of 15-20 DEG C and obtains parent species.
Preferably, the temperature of the PDA culture medium is 15-20 DEG C, preferably 17-19 DEG C.
The typical but non-limiting temperature of PDA culture medium is, for example, 15 DEG C, 16 DEG C, 17 DEG C, 18 DEG C, 19 DEG C or 20 DEG C.
The temperature of PDA culture medium of the present invention is 15-20 DEG C, is more advantageous to obtain good edible mushroom parent species.
Preferably, the liquid culture medium passes through sterilization treatment；
Preferably, the pressure of the sterilizing is 0.14-0.16MPa, preferably 0.15-0.16MPa；
The typical but non-limiting pressure of sterilizing is, for example, 0.14MPa, 0.15MPa or 0.16.
Preferably, the temperature of the sterilizing is 118-121 DEG C, more preferably 119-120 DEG C；
The temperature of sterilizing it is typical but non-limiting be, for example, 118 DEG C, 118.5 DEG C, 119 DEG C, 119.5 DEG C, 120 DEG C,
120.5 DEG C or 121 DEG C.
Preferably, the time of the sterilizing is 40-50min, more preferably 43-46min.
The time of sterilizing it is typical but non-limiting be, for example, 40min, 41min, 42min, 43min, 44min, 45min,
46min, 47min, 48min, 49min or 50min.
The present invention is under conditions of the temperature of sterilizing is 118-121 DEG C, the pressure of sterilizing is 0.14-0.16MPa to liquid
Medium sterilization 40-50min can more effectively kill miscellaneous bacteria, provide better growing environment for the mycelial growth of edible mushroom.
Preferably, the edible mushroom parent species carry out shaken cultivation in shaking flask；
It is highly preferred that the temperature of the shaken cultivation is 20-23 DEG C, it is still more preferably 21-22 DEG C；
The typical but non-limiting temperature of shaken cultivation is, for example, 20 DEG C, 20.5 DEG C, 21 DEG C, 21.5 DEG C, 22 DEG C, 22.5
DEG C or 23 DEG C.
It is highly preferred that the time of the shaken cultivation is 5-7 days, it is still more preferably 6-7 days.
The typical but non-limiting time of shaken cultivation is, for example, 5 days, 6 days or 7 days.
By the way that edible mushroom parent species, shaken cultivation 5-7 days under conditions of 20-23 DEG C, shaking flask covers with mycelia.
It preferably, further include the fermentor that the mycelium is linked into sterilizing after the mycelium for obtaining edible mushroom
In the step of being cultivated；
Preferably, the pressure of the fermentor sterilizing is 0.11-0.13MPa, more preferably 0.11-0.12MPa；
The typical but non-limiting pressure of fermentor sterilizing is, for example, 0.11MPa, 0.12MPa or 0.13MPa.
Preferably, the time of the fermentor sterilizing is 35-45min, more preferably 38-42min；
Fermentor sterilizing time it is typical but non-limiting be, for example, 35min, 36min, 37min, 38min, 39min,
40min, 41min, 42min, 43min, 44min or 45min.
Preferably, the time that the liquid mycelium is cultivated in the fermenter is 3-5 days, more preferably 4-5 days；
The typical but non-limiting time that liquid mycelium is cultivated in the fermenter is, for example, 3 days, 4 days or 5 days.
Preferably, the liquid culture medium in the fermentor, is prepared by the component of following parts by weight:
90-100 parts of wheat bran water, 0.5-0.8 parts of glucose, 0.4-0.7 parts of white sugar and 0.02-0.05 parts of edible oil.
If industrialization production, liquid fermentation tank culture is needed.The technological process of production of liquid fermentation tank: fermentor it is clear
Wash, check-boil ullage disappear-ingredient, feeding-sterilizing-cooling inoculation-is cultivated-connect bacterium bag.Liquid fermentation tank is cleaned up, and
To each switch valve check it is errorless after, sky is carried out to fermentor and is disappeared, adds water in fermentor outer layer, then heats, work as internal layer
When pressure reaches 0.1PA, set temperature, the time is set 30-40 minutes, is opened simultaneously three switches that outer layer is connected to internal layer, is used
Vapor sterilizes internal layer and pipeline 30-40 minutes.In the addition clear water of fermentor, in the ratio of fermenter volume, by wheat bran
It is installed with cloth bag, after temperature boils 25-35 minutes for 100 degree, well-done wheat bran water, glucose, white sugar and edible oil is added together
Enter in fermentor, sterilizes 35-45 minutes at 0.11-0.13MPa, principle disappears consistent with sky.After sterilizing, it is cooled to 22-24
DEG C when keep constant temperature, the liquid spawn in the shaking flask made is accessed into fermentor under aseptic conditions.Then air is opened to open
It closes, air blowing stirring is carried out to liquid in fermentor with filtrated air, the various cultures of liquid bacteria are completed after 3-5 days.
Preferably, the humidity of the solid medium is 60%-68%, preferably 64%-66%；
The humidity of solid medium it is typical but non-limiting be, for example, 60%, 62%, 63%, 64%, 65%,
66%, 67% or 68%.
Preferably, the sterilising temp of the solid medium is 118-121 DEG C, more preferably 119-129 DEG C；
The typical but non-limiting sterilising temp of solid medium is, for example, 118 DEG C, 118.5 DEG C, 119 DEG C, 119.5
DEG C, 120 DEG C, 120.5 DEG C or 121 DEG C.
Preferably, the sterilization pressure of the solid medium is 0.14-0.16MPa, more preferably 0.15-0.16MPa；
The typical but non-limiting sterilization pressure of solid medium is, for example, 0.14MPa, 0.15MPa or 0.16MPa.
Preferably, the sterilization time of the solid medium is 2.5-3.5h, more preferably 2.8-3.2h.
The sterilization time of solid medium it is typical but non-limiting be, for example, 2.5h, 2.6h, 2.7h, 2.8h, 2.9h,
3h, 3.1h, 3.2h, 3.3h, 3.4h or 3.5h.
Preferably, the temperature of the bifurcation fermentation is 15-22 DEG C, preferably 18-20 DEG C；
The typical but non-limiting temperature of bifurcation fermentation is, for example, 15 DEG C, 16 DEG C, 17 DEG C, 18 DEG C, 19 DEG C, 20 DEG C, 21
DEG C or 22 DEG C.
Preferably, the time of the bifurcation fermentation is 15-20 days, more preferably 18-20 days.
The typical but non-limiting time of bifurcation fermentation is, for example, 15 days, 16 days, 17 days, 18 days, 19 days or 20 days.
Bifurcation is fermented 15-20 days under the conditions of 15-22 DEG C of temperature in the present invention, and nutrition edible mushroom more abundant can be obtained
Powder is more conducive to improve the immunity of human body.
The powder of edible fungus that the preparation method of powder of edible fungus as described above obtains.Its nutrition with higher, can be improved people
The immunity of body.
Below with reference to specific embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of powder of edible fungus, comprising the following steps:
(a) with adhesive plaster that its glue is good when will grow intact, strong hickory chick picking, miscellaneous bacteria is prevented, in shady and cool environment
Under cool 3 days, by organize isolated method by hickory chick stem and bacterium emit between tissue remove one under sterile environment
Fritter is put into produced PDA culture medium and cultivates, and culture obtains parent species in the environment of 15 DEG C；
(b) fluid nutrient medium is prepared, culture solution will be made and poured into shaking flask, bottleneck, inoculation mouth is sealed, in pressure
0.14MPa, sterilize at 118 DEG C of temperature 50min, takes out shaking flask after sterilizing, after cooling, accesses parent species one in an aseptic environment
Root places shaking flask on shaking table, and temperature control is cultivated in 23 DEG C of shakes, and after culture 5 days, shaking flask covers with mycelia；
Wherein, liquid culture medium is prepared by the component of following parts by weight: 150 parts of potato, 250 parts of wheat bran, grape
15 parts and 1600 parts of water of sugar；
The preparation method of liquid culture medium, comprising the following steps: part water is added in potato, boils 25 minutes, with filter screen mistake
Filter, wheat bran are installed with cloth bag, and obtained wheat bran water is added in potato water after boiling 35 minutes, and glucose is added, boils 20 minutes, and
Uniform stirring obtains liquid culture medium；
(c) by 20 parts of semen sojae atricolor, 20 parts of corn, 5 parts of jujube, 20 parts of wheat, 20 parts of soya bean through over cleaning, 0.8 part of addition is red
Sugar is mixed, and solid medium is made, and then humid control is 60%, after the sack pack of 15cm × 28cm, in temperature
It 118 DEG C, sterilizes under the conditions of pressure 0.14MPa 2.5 hours, after cooling, with the inoculating gun of liquid spawn fermentation tank in sterile item
It is inoculated under part, hickory chick bacterium liquid strain is accessed in five cereals solid medium, is trained at a temperature of 22 DEG C after inoculation
It supports 15 days.Then bacterium bag bifurcation fermented obtains hickory chick powder by drying, pulverizing.
Embodiment 2
A kind of preparation method of powder of edible fungus, comprising the following steps:
(a) with adhesive plaster that its glue is good when will grow intact, strong hickory chick picking, miscellaneous bacteria is prevented, in shady and cool environment
Under cool 5 days, by organize isolated method by hickory chick stem and bacterium emit between tissue remove one under sterile environment
Fritter is put into produced PDA culture medium and cultivates, and culture obtains parent species in the environment of 20 DEG C；
(b) fluid nutrient medium is prepared, culture solution will be made and poured into shaking flask, bottleneck, inoculation mouth is sealed, in pressure
0.16MPa, sterilize at 121 DEG C of temperature 50min, takes out shaking flask after sterilizing, after cooling, accesses parent species one in an aseptic environment
Root.Shaking flask is placed on shaking table, temperature control is cultivated in 23 DEG C of shakes, and after culture 7 days, shaking flask covers with mycelia；
Wherein, liquid culture medium is prepared by the component of following parts by weight: 250 parts of potato, 160 parts of wheat bran, grape
25 parts and 2500 parts of water of sugar；
The preparation method of liquid culture medium, comprising the following steps: part water is added in potato, boils 35 minutes, with filter screen mistake
Filter, wheat bran are installed with cloth bag, and obtained wheat bran water is added in potato water after boiling 35 minutes, and glucose is added, boils 30 minutes, and
Uniform stirring obtains liquid culture medium；
(c) by 28 parts of semen sojae atricolor, 30 parts of corn, 10 parts of jujube, 30 parts of wheat, 30 parts of soya bean through over cleaning, 1.5 parts of addition is red
Sugar is mixed, and solid medium is made, and then humid control is 68%, after the sack pack of 18cm × 25cm, in temperature
It 121 DEG C, sterilizes under the conditions of pressure 0.16MPa 3.5 hours, after cooling, with the inoculating gun of liquid spawn fermentation tank in sterile item
It is inoculated under part, hickory chick bacterium liquid strain is accessed in five cereals solid medium, is trained at a temperature of 21 DEG C after inoculation
It supports 20 days.Then bacterium bag bifurcation fermented obtains hickory chick powder by drying, pulverizing.
Embodiment 3
A kind of preparation method of powder of edible fungus, comprising the following steps:
(a) with adhesive plaster that its glue is good when will grow intact, strong bolete picking, miscellaneous bacteria is prevented, in shady and cool environment
Under cool 4 days, by organize isolated method by bolete stem and bacterium emit between tissue remove one under sterile environment
Fritter is put into produced PDA culture medium and cultivates, and culture obtains parent species in the environment of 18 DEG C；
(b) fluid nutrient medium is prepared, culture solution will be made and poured into shaking flask, bottleneck, inoculation mouth is sealed, in pressure
0.15MPa, sterilize at 120 DEG C of temperature 45min, takes out shaking flask after sterilizing, after cooling, accesses parent species one in an aseptic environment
Root.Shaking flask is placed on shaking table, temperature control is cultivated in 21 DEG C of shakes, and after culture 6 days, shaking flask covers with mycelia；
Wherein, liquid culture medium is prepared by the component of following parts by weight: 180 parts of potato, 230 parts of wheat bran, grape
18 parts and 1800 parts of water of sugar；
The preparation method of liquid culture medium, comprising the following steps: part water is added in potato, boils 30 minutes, with filter screen mistake
Filter, wheat bran are installed with cloth bag, and obtained wheat bran water is added in potato water after boiling 30 minutes, and glucose is added, boils 25 minutes, and
Uniform stirring obtains liquid culture medium；
Liquid fermentation tank is cleaned up, and to each switch valve check it is errorless after, sky is carried out to fermentor and is disappeared,
Fermentor outer layer adds water, then heats, and when internal pressure reaches 0.1PA, set temperature, the time is set 30 minutes, beats simultaneously
Over layer be connected to three of internal layer switches, internal layer and pipeline are sterilized 40 minutes with vapor.In the addition clear water of fermentor, press
The ratio of fermenter volume installs wheat bran with cloth bag, after temperature boils 35 minutes for 100 DEG C, by well-done wheat bran water, grape
Sugar, white sugar and edible oil are added in fermentor together, are sterilized 45 minutes at 0.11MPa, principle disappears consistent with sky.Sterilizing
Afterwards, constant temperature is kept when being cooled to 22 DEG C, the liquid spawn in the shaking flask made is accessed into fermentor under aseptic conditions, then
Air switch is opened, air blowing stirring is carried out to liquid in fermentor with filtrated air, strain cultivation is completed after 5 days；
(c) by 24 parts of semen sojae atricolor, 23 parts of corn, 7 parts of jujube, 22 parts of wheat, 23 parts of soya bean and through over cleaning, be added 1 part it is red
Sugar is mixed, and solid medium is made, and then humid control is in 60%-68%, after the sack pack of 15cm × 28cm,
It 118-121 DEG C of temperature, sterilizes under the conditions of pressure 0.14-0.16MPa 2.5-3.5 hour, after cooling down, uses liquid spawn fermentation tank
Inoculating gun be aseptically inoculated with, by bolete bacterium liquid strain access five cereals solid medium in, after inoculation
Culture 15-20 days is carried out at a temperature of 15-22 DEG C.Then bacterium bag bifurcation fermented obtains ox by drying, pulverizing
Liver bacterium powder.
Embodiment 4
A kind of preparation method of powder of edible fungus, comprising the following steps:
(a) with adhesive plaster that its glue is good when will grow intact, strong hickory chick picking, miscellaneous bacteria is prevented, in shady and cool environment
Under cool 4 days, by organize isolated method by hickory chick stem and bacterium emit between tissue remove one under sterile environment
Fritter is put into produced PDA culture medium and cultivates, and culture obtains parent species in the environment of 18 DEG C；
(b) fluid nutrient medium is prepared, culture solution will be made and poured into shaking flask, bottleneck, inoculation mouth is sealed, in pressure
0.15MPa, sterilize at 119 DEG C of temperature 48min, takes out shaking flask after sterilizing, after cooling, accesses parent species one in an aseptic environment
Root.Shaking flask is placed on shaking table, temperature control is cultivated in 22 DEG C of shakes, and after culture 6 days, shaking flask covers with mycelia；
Wherein, liquid culture medium is prepared by the component of following parts by weight: 220 parts of potato, 190 parts of wheat bran, grape
22 parts and 2200 parts of water of sugar；
The preparation method of liquid culture medium, comprising the following steps: part water is added in potato, boils 32 minutes, with filter screen mistake
Filter, wheat bran are installed with cloth bag, and obtained wheat bran water is added in potato water after boiling 28 minutes, and glucose is added, boils 25 minutes, and
Uniform stirring obtains liquid culture medium；
Liquid fermentation tank is cleaned up, and to each switch valve check it is errorless after, sky is carried out to fermentor and is disappeared,
Fermentor outer layer adds water, then heats, and when internal pressure reaches 0.1PA, set temperature, the time is set 30 minutes, beats simultaneously
Over layer be connected to three of internal layer switches, internal layer and pipeline are sterilized 40 minutes with vapor.In the addition clear water of fermentor, press
The ratio of fermenter volume installs wheat bran with cloth bag, after temperature boils 25-35 minutes for 100 DEG C, by well-done wheat bran water, Portugal
Grape sugar, white sugar and edible oil are added in fermentor together, are sterilized 40 minutes at 0.12MPa, principle disappears consistent with sky.Sterilizing
Afterwards, constant temperature is kept when being cooled to 22 DEG C, the liquid spawn in the shaking flask made is accessed into fermentor under aseptic conditions, then
Air switch is opened, air blowing stirring is carried out to liquid in fermentor with filtrated air, the various cultures of liquid bacteria are completed after 5 days；
(c) solid-state five cereals culture medium is fabricated to bacterium bag: by 26 parts of semen sojae atricolor, 27 parts of corn, 9 parts of jujube, 26 parts of wheat,
27 parts of soya bean and through over cleaning, is added 1.3 portions of brown sugar and is mixed, solid medium is made, then humid control is in 60%-
68%, after the sack pack of 15cm × 28cm, sterilize under the conditions of 120 DEG C of temperature, pressure 0.14MPa 3.2 hours, it is cooling
Afterwards, it is aseptically inoculated with the inoculating gun of liquid spawn fermentation tank, hickory chick bacterium liquid strain access five cereals is consolidated
Culture 18 days is carried out in state culture medium, after inoculation at a temperature of 17 DEG C.Then bacterium bag bifurcation fermented, by drying, surpassing
Crushing of Ultrafine or briquetting obtain Morchella esculenta (L.) Pers mycelium bacterium powder.
Embodiment 5
A kind of preparation method of powder of edible fungus, except liquid culture medium by 200 parts of potato, 200 parts of wheat bran, 19 parts of glucose and
2000 parts of water are prepared, and solid medium is by 25 parts of semen sojae atricolor, 24 parts of corn, 8 parts of jujube, 23 parts of wheat, 24 parts of soya bean and sugar
1.2 parts are prepared, other operating procedures are same as Example 1.
The present invention is fermented by bifurcation combines the nutrition of five cereals with the nutrition of powder of edible fungus, obtains having higher
Nutritive value, the powder of edible fungus of body immunity can be improved.
Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that: its according to
So be possible to modify the technical solutions described in the foregoing embodiments, or to some or all of the technical features into
Row equivalent replacement；And these are modified or replaceed, various embodiments of the present invention technology that it does not separate the essence of the corresponding technical solution
The range of scheme.
Claims (10)
1. a kind of preparation method of powder of edible fungus, which comprises the following steps:
The mycelium for the edible mushroom that liquid culture medium culture parent species are obtained is inoculated into solid together together with the liquid culture medium
Bifurcation fermented and cultured is carried out in state culture medium, then dry, crushing obtains powder of edible fungus；
The liquid culture medium is prepared by the component for including following parts by weight:
150-250 parts of potato, 160-250 parts of wheat bran, 15-25 parts of glucose and 1600-2500 parts of water；
The solid medium is prepared by the component for including following parts by weight:
0.8-1.5 parts of 20-28 parts of semen sojae atricolor, 20-30 parts of corn, 5-10 parts of jujube, 20-30 parts of wheat, 20-30 parts of soya bean and sugar；
The edible mushroom includes at least one of hickory chick, matsutake, bolete and truffle.
2. the preparation method of powder of edible fungus according to claim 1, which is characterized in that the liquid culture medium, by including
The component of following parts by weight is prepared:
180-220 parts of potato, 190-230 parts of wheat bran, 18-22 parts of glucose and 1800-2200 parts of water.
3. the preparation method of powder of edible fungus according to claim 1, which is characterized in that the solid medium, by including
The component of following parts by weight is prepared:
1-1.3 parts of 24-26 parts of semen sojae atricolor, 23-27 parts of corn, 7-9 parts of jujube, 22-26 parts of wheat, 23-27 parts of soya bean and sugar.
4. the preparation method of powder of edible fungus according to claim 1, which is characterized in that the liquid culture medium culture parent species
The mycelial process of obtained edible mushroom, specifically includes the following steps: the thallus tissue cultures of edible mushroom is taken to obtain parent species, it will
It is cultivated in liquid culture medium after parent species access sterilizing, obtains the mycelium of edible mushroom；
Preferably, the thallus group of the edible mushroom, which is woven in PDA culture medium, cultivates, and obtains parent species；
Preferably, the cultivation temperature in the PDA culture medium is 15-20 DEG C.
5. the preparation method of powder of edible fungus according to claim 4, which is characterized in that the liquid culture medium is by sterilizing
Processing；
Preferably, the pressure of the sterilizing is 0.14-0.16MPa, and temperature is 118-121 DEG C, time 40-50min；
Preferably, the parent species carry out shaken cultivation in shaking flask；
It is highly preferred that the temperature of the shaken cultivation is 20-23 DEG C, the time of culture is 5-7 days.
6. the preparation method of powder of edible fungus according to claim 4, which is characterized in that the mycelium for obtaining edible mushroom
Afterwards, it is inoculated into before solid medium, further includes the steps that for the mycelium being linked into the fermentor of sterilizing and cultivate；
Preferably, the pressure of the sterilizing of the fermentor is 0.11-0.13MPa, and the time of sterilizing is 35-45min；
Preferably, the time that the liquid mycelium is cultivated in the fermenter is 3-5 days；
Preferably, the liquid culture medium in the fermentor, is prepared by the component of following parts by weight:
90-100 parts of wheat bran water, 0.5-0.8 parts of glucose, 0.4-0.7 parts of white sugar and 0.02-0.05 parts of edible oil.
7. the preparation method of powder of edible fungus according to claim 1, which is characterized in that the humidity of the solid medium is
60%-68%；
Preferably, the sterilising temp of the solid medium is 118-121 DEG C, sterilization pressure 0.14-0.16MPa, when sterilizing
Between be 2.5-3.5h.
8. the preparation method of powder of edible fungus according to claim 1, which is characterized in that the temperature of bifurcation fermentation is
15-22℃。
9. the preparation method of powder of edible fungus according to claim 1, which is characterized in that the time of bifurcation fermentation is
15-20 days.
10. the powder of edible fungus obtained such as the preparation method of the described in any item powder of edible fungus of claim 1-9.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111869820A (en) * | 2020-07-31 | 2020-11-03 | 廊坊师范学院 | Processing method and application of millet raw material |
| CN113068824A (en) * | 2021-04-25 | 2021-07-06 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | High-protein composite nutritional coarse cereal edible fungus powder food and preparation method thereof |
| CN113475674A (en) * | 2021-08-03 | 2021-10-08 | 内蒙古中谷君创生物科技发展有限责任公司 | Method for producing nutritional rice flour by using hypsizygus marmoreus mycelia |
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| CN103393095A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Processing method of compound hypha powder |
| CN107586724A (en) * | 2017-08-29 | 2018-01-16 | 殷东林 | A kind of mycelial cultural method of Antrodia camphorata |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103393095A (en) * | 2013-07-19 | 2013-11-20 | 乳山市华隆生物科技有限公司 | Processing method of compound hypha powder |
| CN107586724A (en) * | 2017-08-29 | 2018-01-16 | 殷东林 | A kind of mycelial cultural method of Antrodia camphorata |
| CN109076882A (en) * | 2018-06-29 | 2018-12-25 | 江西天和食用菌开发有限公司 | A kind of mycelial cultural method of selenium-enriched edible mushroom and its application |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111869820A (en) * | 2020-07-31 | 2020-11-03 | 廊坊师范学院 | Processing method and application of millet raw material |
| CN113068824A (en) * | 2021-04-25 | 2021-07-06 | 宁夏农产品质量标准与检测技术研究所(宁夏农产品质量监测中心) | High-protein composite nutritional coarse cereal edible fungus powder food and preparation method thereof |
| CN113475674A (en) * | 2021-08-03 | 2021-10-08 | 内蒙古中谷君创生物科技发展有限责任公司 | Method for producing nutritional rice flour by using hypsizygus marmoreus mycelia |
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