CN109276515B - Composition containing bioactive collagen peptide and preparation method and application thereof - Google Patents
Composition containing bioactive collagen peptide and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a composition containing bioactive collagen peptide and a preparation method and application thereof, the composition is of a W/O/W type multiple emulsion structure, the composition of an internal water phase comprises a bioactive collagen peptide cosolvent and an accelerant, and the cosolvent and the accelerant are commercially available cosmetic-grade products; the preparation method of the composition comprises the following steps: mixing and dissolving the bioactive collagen peptide, the cosolvent and the accelerant to obtain an inner water phase; dissolving an emulsifier I in grease to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing under ultrahigh pressure to obtain W/O emulsion; (2) dissolving an emulsifier II in water to obtain an external water phase; slowly adding the W/O emulsion obtained in the step (1) into an external water phase, and homogenizing under ultrahigh pressure to obtain W/O/W type multiple emulsion; the composition cosmetic containing the bioactive collagen peptide can preserve moisture, resist oxidation, resist aging, remove wrinkles and enhance skin elasticity.
Description
Technical Field
The invention relates to the technical field of daily cosmetics, in particular to a composition containing bioactive collagen peptide and a preparation method and application thereof.
Background
Collagen is widely present in animal bone, tendon, cartilage and skin and other connective tissues, is a main component of extracellular matrix (ECM), accounts for about 85% of the solid substance of animal collagen fiber, accounts for 25-30% of the total amount of protein in vivo, and has functions of supporting organs and protecting organism. Collagen, as a kind of fibrin, has a basic constituent unit of a procollagen molecule. The procollagen molecule is a fibrous protein formed by winding three strands of alpha-helical peptide chains, has a length of about 300nm, a diameter of about 1.5nm, and a molecular weight of 300 kDa. Collagen contains a large amount of glycine, which accounts for about 1/3 of total amino acid residues, and the primary structures (peptide chains) of the glycine are (Gly-X-Y) n repeated sequences; secondly, proline and hydroxyproline with the content of 15-30 percent are peculiar to the collagen, but the content of essential amino acids such as tryptophan, tyrosine and methionine is low, so the collagen belongs to incomplete protein from the nutrition perspective.
Because the collagen and the hydrolysate thereof have similar structures with the skin collagen of human, good compatibility, can enter the deep layer of human skin, has good nutrition function to the skin, and the collagen contains a great deal of amino acids such as hydroxyproline, hydroxylysine and the like with carboxyl and hydroxyl, and a great deal of natural moisturizing factors such as glycine and the like, can improve the water storage capacity of tissue cells, has good moisturizing effect to the skin, and a great deal of collagen polypeptides are related to the growth, division, proliferation, migration and differentiation of skin cells, can provide nutrients to the skin, delay the aging of the skin, promote the wound repair of the skin, and the collagen solution has stronger radiation resistance, thereby being widely applied to the cosmetic industry,
however, not all collagens or hydrolysates thereof are suitable for skin care products, and physiologically active type I collagen is generally used. However, type I collagen has non-helical domains called telopeptides at both ends, and the telopeptides are a major cause of immune response. In fact, when type I collagen is used as a raw material for pharmaceuticals, cosmetics, or the like, it is preferable to use atelocollagen by removing telopeptides. However, the I-terminal peptide collagen peptide having bioactivity has poor self-stability, and the temperature, pH, ionic strength, etc. all affect the bioactivity of the collagen peptide.
Disclosure of Invention
Aiming at the technical problems, the invention provides a composition containing bioactive collagen peptide, a preparation method and application thereof.
The technical scheme of the invention is as follows: a composition containing bioactive collagen peptide is of a W/O/W type multiple emulsion structure, the D50 particle size of the W/O/W type multiple emulsion structure is 3-12 mu m, and the mass ratio of an internal water phase, an oil phase and an external water phase is (5-11): 11-21): 21-26;
the inner water phase consists of bioactive collagen peptide, cosolvent and accelerant according to the mass ratio of 1 (2-5) to (2-5);
the oil phase contains grease and an emulsifier I, the mass ratio of the emulsifier I to the grease is 1 (5-25), and the emulsifier I is a polyglycerol ester emulsifier;
the external water phase contains an emulsifier II, and the mass ratio of the emulsifier II to water is 1 (10-30);
the bioactive collagen peptide is I-type ATELOCOLLAGEN, the INCI name of the bioactive collagen peptide is ATELOCOLLAGEN, the molecular weight of the bioactive collagen peptide is 800-1800 daltons, the bioactive collagen peptide is derived from codaceae fish skin protein, and the bioactive collagen peptide is purchased from green functional daily chemical product innovation center of south China cooperative innovation institute;
the cosolvent is one or more of 1, 3-butanediol, xylitol, lactitol and glycerol, and is a commercially available cosmetic grade product;
the accelerant is one or more of poly alpha-glucan oligosaccharide, galactose arabinan, erythrulose, fructo-oligosaccharide, mannan, levan, succinoglycan, raffinose, glucan, myristoyl/PCA chitosan, trichlorogalactose, hydrolyzed xylan, fucose, glycosyl trehalose and rhamnose, and the accelerant is a commercially available cosmetic grade product.
Further, the oil and fat is selected from one or any mixture of hydrogenated polyisobutene, cetyl ethyl hexanoate, glycerol tri (ethyl hexanoate), octyl dodecanol myristate, diisostearyl malate and di (octyl dodecanol) malate; the emulsifier I is a mixture of any two emulsifiers in polyglycerol-3 decanoate, polyglycerol-4 oleate, polyglycerol-5 trioleate, polyglycerol-5 isostearate, polyglycerol-6 dioleate, polyglycerol-6 stearate, polyglycerol-6 isostearate and polyglycerol-6 pentaoleate in any mass ratio.
Further, the emulsifier II is a mixture of any two emulsifiers of C8-C14 alkyl glucoside, C12-18 alkyl glucoside, sucrose pentahydroxystearate, sucrose tribehenate, sodium lauroyl lactylate, isostearyl lactate, distarch phosphate, oleyl polyether-5 phosphate and triolyl polyether-8 phosphate in any mass ratio.
Furthermore, the composition containing the bioactive collagen peptide contains a high molecular polymer dispersing agent, the high molecular polymer dispersing agent is selected from one or any mixture of acrylic acid (ester) copolymer, acrylic acid (ester) copolymer AMP salt, ammonium acryloyldimethyl taurate/steareth-8 methacrylate copolymer, steareth-10 allyl ether/acrylic acid (ester) copolymer and beheneth-25 methacrylate cross-linked polymer, and the content of the high molecular polymer dispersing agent is 0.05-0.08% of the mass of the composition.
Furthermore, the internal water phase also comprises plant effective components, and the mass ratio of the plant effective components to the bioactive collagen peptide, the cosolvent and the accelerant is (2-5) to (2-5) 1 to (2-5).
A method for preparing a composition containing bioactive collagen peptide comprises the following steps:
(1) mixing and dissolving the bioactive collagen peptide, the cosolvent and the accelerant to obtain an inner water phase; dissolving an emulsifier I in grease to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing under ultrahigh pressure to obtain W/O emulsion with the particle size of 0.7-1.0 mu m; the pressure of ultrahigh pressure homogenization is 1100-1300 bar; the time is 15-30 min; the temperature is 0-25 ℃;
(2) dissolving an emulsifier II in water to obtain an external water phase; slowly adding the W/O emulsion obtained in the step (1) into the external water phase, and homogenizing under ultrahigh pressure to obtain W/O/W type multiple emulsion.
Further, the effective components of the plants comprise the following raw materials in percentage by weight: 5-12% of witch hazel extract, 6-11% of tropaeolum extract, 2-4% of maca extract, 3-9% of almond extract, 2-12% of ginseng extract, 8-15% of aloe extract, 3-9% of polygala fallax hemsl extract, 2-8% of philippine flemingia root extract, 1-5% of dulcis japonicas extract and 0.8-2% of cerasus humilis kernel protein polypeptide.
Further, the cerasus humilis kernel protein polypeptide is prepared by the steps of pretreatment, cerasus humilis kernel protein modification, enzymolysis, enzyme deactivation, concentration and purification.
A method for preparing a composition containing bioactive collagen peptide comprises the following steps:
(1) mixing and dissolving bioactive collagen peptide, plant effective components, cosolvent and accelerant to obtain an internal water phase; dissolving an emulsifier I in grease to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing under ultrahigh pressure to obtain W/O emulsion with the particle size of 0.7-1.0 mu m; the pressure of the ultrahigh pressure homogenization is 1100-1300 bar; the time is 15-30 min; the temperature is 0-25 ℃;
(2) dissolving an emulsifier II in water to obtain an external water phase; slowly adding the W/O emulsion obtained in the step (1) into the external water phase, and homogenizing under ultrahigh pressure to obtain W/O/W type multiple emulsion.
The composition is used for preparing cosmetics; in the present invention, cosmetics include, but are not limited to, creams, eye creams, lotions, essences, essence creams, masks, toners, and the like. The composition cosmetic containing the bioactive collagen peptide can preserve moisture, resist oxidation, resist aging, remove wrinkles and enhance skin elasticity.
Compared with the prior art, the invention has the beneficial effects that:
(1) in the composition of the bioactive collagen peptide, the bioactive collagen peptide belongs to type I atelocollagen, and the bioactivity is high;
(2) the W/O/W multiple emulsion structure realizes the protection and slow release of the bioactive collagen;
(3) the transdermal absorption capacity of the bioactive collagen peptide is improved by the existence of the accelerant in the composition;
(4) the preparation process of the composition is mild, compared with the traditional method of directly adding the bioactive collagen into the formula, the composition can exert the application value of the bioactive collagen to the maximum extent, and has high economical efficiency because the absorption capacity is improved and high-concentration addition is not needed.
(5) The composition containing the bioactive collagen peptide prepared by the extraction method has good moisturizing capability, can remove hydroxyl free radicals and DPPH free radicals, improves the nutritional capability of skin, has activities of resisting oxidation and allergy, improving skin tolerance, resisting aging, removing wrinkles, enhancing skin elasticity and the like, can be used in cosmetics, and can be prepared into a W/O/W type multiple emulsion structure, so that the bioactive collagen can be protected and slowly released, and the activity of the bioactive collagen can be improved.
Drawings
FIG. 1 is a graph showing the change in moisture content of stratum corneum in a test for improving moisture retention and elasticity using a composition of bioactive collagen of the present invention.
FIG. 2 is a graph showing the change in the amount of water lost through the skin in the moisture retention and elasticity test using the composition of bioactive collagen of the present invention.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
(1) Mixing and dissolving 1 part by mass of bioactive collagen, 2 parts by mass of 1, 3-butanediol and 2 parts by mass of erythrulose to obtain an internal water phase; dissolving 0.5 parts by mass of polyglycerol-3 decanoate and 0.5 parts by mass of polyglycerol-6 pentaoleate in 10 parts by mass of hydrogenated polyisobutene to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1100bar and 20 deg.C for 15min to obtain W/O emulsion with particle size of 0.7 μm;
(2) dissolving 0.5 parts by mass of C8-C14 alkyl glucoside and 0.5 parts by mass of sucrose pentahydroxystearate in 20 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1100bar and 20 deg.C for 15min, and adding 0.0185 mass parts of acrylic acid (ester) copolymer AMP salt to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.70%.
The particle size of the W/O/W emulsion was 8.0. mu.m as measured by a laser particle size analyzer.
Example 2
(1) Mixing and dissolving 1 part by mass of bioactive collagen, 3 parts by mass of lactitol and 4 parts by mass of fructo-oligosaccharide to obtain an inner water phase; dissolving 0.5 part by mass of polyglycerol-4 oleate and 0.5 part by mass of polyglycerol-5 trioleate in 12 parts by mass of cetyl ethylhexanoate to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1200bar and 20 deg.C for 20min to obtain W/O emulsion with particle size of 0.85 μm;
(2) dissolving 0.5 part by mass of C12-18 alkyl glucoside and 0.5 part by mass of sucrose tribehenate in 25 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1200bar and 20 deg.C for 20min, and adding 0.0282 parts by mass of ammonium acryloyldimethyl taurate/steareth-8 methacrylate copolymer to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.13%.
The particle size of the W/O/W emulsion, as measured by a laser particle size instrument, was 8.8. mu.m.
Example 3
(1) Mixing and dissolving 1 part by mass of bioactive collagen, 5 parts by mass of xylitol and 4 parts by mass of hydrolyzed xylan to obtain an internal water phase; dissolving 0.5 parts by mass of polyglycerol-5 isostearate and 0.5 parts by mass of polyglycerol-6 stearate in 20 parts by mass of glycerol tri (ethyl hexanoate) to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1300bar and 20 deg.C for 30min to obtain W/O emulsion with particle size of 0.82 μm;
(2) dissolving 0.5 part by mass of sodium lauroyl lactylate and 0.5 part by mass of oleyl polyether-5 phosphate in 25 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1300bar and 20 deg.C for 30min, and adding 0.0456 mass parts of steareth-10 allyl ether/acrylic acid (ester) copolymer to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 1.75%.
The particle size of the W/O/W emulsion, as measured by a laser particle size instrument, was 9.2. mu.m.
Example 4
(1) Mixing and dissolving 1 part by mass of bioactive collagen, 5 parts by mass of glycerol and 2 parts by mass of raffinose to obtain an internal water phase; dissolving 0.5 parts by mass of polyglycerol-3 decanoate and 0.5 parts by mass of polyglycerol-6 isostearate in 15 parts by mass of diisostearyl malate to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1250bar and 20 deg.C for 20min to obtain W/O emulsion with particle size of 1.0 μm;
(2) dissolving 0.5 part by mass of isostearyl alcohol lactate and 0.5 part by mass of sucrose tribehenate in 22 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1250bar at 20 deg.C for 20min, and adding 0.0456 mass parts of acrylic acid (ester) copolymer to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.13%.
The particle size of the W/O/W emulsion, as measured by a laser particle size instrument, was 10.0. mu.m.
Example 5
(1) Mixing and dissolving 1 part by mass of bioactive collagen, 3 parts by mass of traditional Chinese medicine bioactive peptide, 5 parts by mass of 1, 3-butanediol and 5 parts by mass of alpha-glucan oligosaccharide to obtain an internal water phase; dissolving 0.5 parts by mass of polyglycerol-6 dioleate and 0.5 parts by mass of polyglycerol-6 stearate in 20 parts by mass of di (octyldodecanol) malate to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1100bar and 20 deg.C for 15min to obtain W/O emulsion with particle size of 0.75 μm;
(2) dissolving 0.5 part by mass of sucrose pentahydroxystearate and 0.5 part by mass of double starch phosphate in 25 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1100bar and 20 deg.C for 15min, and adding 0.0464 parts by mass of behenyl alcohol polyether-25 methacrylate cross-linked polymer to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 1.72%.
The particle size of the W/O/W emulsion, as measured by a laser particle size instrument, was 9.8. mu.m.
Example 6
(1) Mixing and dissolving 1 part by mass of bioactive collagen, 3 parts by mass of glycerol and 3 parts by mass of fucose to obtain an inner water phase; dissolving 0.5 part by mass of polyglycerol-4 oleate and 0.5 part by mass of polyglycerol-6 dioleate in 18 parts by mass of hydrogenated polyisobutene to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1300bar and 20 deg.C for 30min to obtain W/O emulsion with particle size of 0.85 μm;
(2) dissolving 0.5 parts by mass of C12-18 alkyl glucoside and 0.5 parts by mass of isostearyl lactate in 23 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1300bar and 20 deg.C for 30min, and adding 0.025 mass parts of steareth-10 allyl ether/acrylic acid (ester) copolymer to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.00%.
The particle size of the W/O/W emulsion was 8.0. mu.m as measured by a laser particle size analyzer.
Example 7
In this example, no accelerator was added to the internal aqueous phase, and the remaining steps were the same as in example 1, with the following specific steps:
(1) mixing and dissolving 1 part by mass of bioactive collagen and 2 parts by mass of 1, 3-butanediol to obtain an internal water phase; dissolving 0.5 parts by mass of polyglycerol-3 decanoate and 0.5 parts by mass of polyglycerol-6 pentaoleate in 10 parts by mass of hydrogenated polyisobutene to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1100bar and 20 deg.C for 15min to obtain W/O emulsion with particle size of 0.85 μm;
(2) dissolving 0.5 parts by mass of C8-C14 alkyl glucoside and 0.5 parts by mass of sucrose pentahydroxystearate in 20 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1100bar and 20 deg.C for 15min, and adding 0.0185 mass parts of acrylic acid (ester) copolymer AMP salt to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.86%.
The particle size of the W/O/W emulsion was 8.0. mu.m as measured by a laser particle size analyzer.
Example 8
In this example, the composition was prepared without adding a high molecular weight polymeric dispersant, and the remaining steps were as in example 2, with the following specific steps:
(1) mixing and dissolving 1 part by mass of bioactive collagen, 3 parts by mass of lactitol and 4 parts by mass of fructo-oligosaccharide to obtain an inner water phase; dissolving 0.5 part by mass of polyglycerol-4 oleate and 0.5 part by mass of polyglycerol-5 trioleate in 12 parts by mass of cetyl ethylhexanoate to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1200bar and 20 deg.C for 20min to obtain W/O emulsion with particle size of 0.85 μm;
(2) dissolving 0.5 part by mass of C12-18 alkyl glucoside and 0.5 part by mass of sucrose tribehenate in 25 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1200bar and 20 deg.C for 20min to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.13%.
The particle size of the W/O/W emulsion, as measured by a laser particle size instrument, was 8.8. mu.m.
Example 9
In this example, the plant active ingredient was added to the internal aqueous phase, and the remaining steps were the same as in example 1, and the specific steps were:
(1) mixing and dissolving 1 part by mass of bioactive collagen, 0.5 part by mass of plant active ingredients, 2 parts by mass of 1, 3-butanediol and 2 parts by mass of erythrulose to obtain an internal water phase; dissolving 0.5 parts by mass of polyglycerol-3 decanoate and 0.5 parts by mass of polyglycerol-6 pentaoleate in 10 parts by mass of hydrogenated polyisobutene to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing at 1100bar and 20 deg.C for 15min to obtain W/O emulsion with particle size of 0.6 μm;
(2) dissolving 0.5 parts by mass of C8-C14 alkyl glucoside and 0.5 parts by mass of sucrose pentahydroxystearate in 20 parts by mass of water to obtain an external water phase; slowly adding W/O emulsion into external water phase, homogenizing at 1100bar and 20 deg.C for 15min, and adding 0.0185 mass parts of acrylic acid (ester) copolymer AMP salt to obtain composition containing bioactive collagen with W/O/W emulsion structure.
The content of bioactive collagen is about 2.70%.
The particle size of the W/O/W emulsion, as measured by a laser particle size instrument, was 7.9. mu.m.
Application example 1
Anti-aging serum-type products were prepared using the bioactive collagen-containing composition of the present invention according to the formulations listed in table 1 below.
Table 1: formula of anti-aging essence product
TABLE 1
The preparation process comprises the following steps:
1. preparing a B phase: mixing and stirring the phase B until the phase B is fully soaked for later use;
2. preparing a C phase: mixing the phase C, and stirring until the phase C is completely dissolved uniformly for later use;
3. preparing a G phase: at normal temperature, uniformly stirring HRE-40 and essence, adding propylene glycol, uniformly stirring, adding deionized water, and fully stirring until the mixture is completely dissolved and transparent for later use;
4. slowly spraying carbomer into the rest components in the phase A while carrying out weak homogenization (at the speed of 3000-4000 rpm), stirring and heating while carrying out weak homogenization (at the speed of 3000-4000 rpm) for a plurality of times in a short time till the temperature reaches 82-85 ℃, carrying out weak homogenization (at the speed of 3000-4000 rpm) for 5-8 minutes until the carbomer is completely and uniformly dispersed, adding the completely soaked phase B, stirring until the carbomer is completely and uniformly dissolved, disinfecting for at least 15-20 minutes, defoaming and cooling;
5. cooling to 60-65 ℃, adding the pre-dissolved transparent phase C, and fully and uniformly stirring;
6. cooling to 50-55 ℃, adding the phase D, and fully and uniformly stirring;
7. cooling to below 45 deg.C, adding the pre-dissolved transparent phase E, stirring thoroughly, sequentially adding phase F, stirring thoroughly, adding the prepared phase G, and stirring thoroughly;
8. sampling, detecting, and filtering and discharging after the product is qualified.
Application example 2
Anti-aging cream products were prepared using the bioactive collagen-containing compositions of the present invention according to the formulations listed in table 2 below.
Table 2: formula of anti-aging cream product
TABLE 2
The preparation process comprises the following steps:
1. preparing a B phase: mixing the phase B, and fully and uniformly stirring for later use;
2. preparing a G phase: mixing the dry clean container G, heating to 80 ℃, stirring to dissolve completely, and cooling to 55 ℃ for later use;
3. sequentially adding phase A into a water phase pot, stirring and heating to 60-65 deg.C, adding fully soaked phase B, continuously heating and stirring to 82-85 deg.C, homogenizing for 3-5 min to completely uniform, maintaining the temperature for 20-30 min, sterilizing, defoaming, and cooling;
4. cooling to 55 deg.C, adding phase C, stirring thoroughly until completely dissolved, and cooling;
5. cooling to 45 ℃, sequentially adding D, E, F phases, and respectively stirring until the D, E, F phases are completely dissolved;
6. adding the H phase into an emulsifying pot, stirring at medium speed until uniform particles visible to naked eyes are completely formed, then adding the pre-treated G phase, fully stirring uniformly, and defoaming for later use;
7. slowly dripping the A + B + C + D + E + F phases in the water phase pot into the G + H phases in the emulsifying pot under the weak-speed stirring;
8. after the dripping is finished, the mixture is lifted to the medium speed, stirred for 5-10 minutes, homogenized for 3 minutes at the weak-medium speed (the speed is 3000-4000 rpm), and stirred for 10 minutes at the medium speed;
9. sampling, detecting, filtering and discharging
Application example 3
In application example 1, the composition containing the bioactive collagen was replaced with water, and the remaining components were the same.
Application example 4
In application example 2, the composition containing the bioactive collagen was replaced with water, and the remaining components were the same.
Application example 5
In application example 1, the composition containing bioactive collagen was replaced with a mixture of 0.135% bioactive collagen and 4.865% water, and the remaining components were the same.
Application example 6
In application example 2, 0.1065% bioactive collagen and 4.8935% water were used instead of the composition containing bioactive collagen, and the remaining components were the same.
Test example 1
Stability test
Sensory testing: visually observing the properties of the product to see whether the product is abnormal or not;
heat resistance test: putting the sample into an electric heating constant temperature incubator at the temperature of (40 +/-1) DEG C for 24 hours, and observing whether phenomena such as thinning, discoloration, layering, hardness change and the like exist after the temperature is recovered to judge the heat resistance of the sample;
cold resistance test: putting the sample into a refrigerator at (-5 to-10) DEG C for 24h, and observing whether phenomena such as thinning, color change, layering, hardness change and the like exist after the temperature is recovered to judge the cold resistance of the sample;
and (3) centrifugal test: and (3) placing the sample in a centrifuge, testing for 30min at the rotating speed of 3000r/min, and observing the separation and delamination conditions of the sample.
The stability test results for examples 1-8 are shown in Table 3 below:
table 3: examples 1 to 8 stability test results
TABLE 3
The stability test results of examples 1-8 show that: the compositions containing bioactive collagen prepared in the embodiments 1 to 6 of the invention have good stability, while the compositions prepared in the embodiment 7 have no accelerator and the compositions prepared in the embodiment 8 have no high molecular polymer dispersant, so the compositions have poor stability.
The stability test results of application examples 1-6 are shown in table 4 below:
table 4: application examples 1-6 stability test results
TABLE 4
The stability test results of application examples 1-6 show that: the composition containing the bioactive collagen prepared in the embodiments 1-2 of the invention has good stability in aqueous and cream products; applications 3-6 are based on the absence or substitution of normal bioactive collagen, and no stability problems.
Test example 2
Compositions of bioactive collagen for improved moisture retention and elasticity testing
1) The instrument comprises the following steps: MPA10 multi-probe test system, MPA580 skin elasticity tester, manufactured by COARAGE + KHAZAKA (CK) of Germany.
2) Sample preparation: blank (deionized water), bioactive collagen composition from application example 1, and 2.7% bioactive collagen in water.
The test method comprises the following steps: according to the testing requirements of an MPA10 multi-probe testing system and an MPA580 skin elasticity tester, the number of the tested persons is 9, the tested parts are the inner sides of the forearms of the tested persons, each group (blank group and skin care product group) of the persons is provided with 3 tested parts, the tested parts are smeared according to the dosage of (2.0 +/-0.1) mg samples/cm 2, the initial, 1h, 2h and 4h data are detected, and the data are analyzed by an SPSS20.0 data statistical system.
3) The test results are shown in FIGS. 1 and 2.
The result shows that the composition containing the bioactive collagen has obvious moisturizing effect, and the water replenishing effect and the water locking capacity of the composition on the skin cuticle are both superior to those of a 2.70 percent bioactive collagen aqueous solution.
Test example 3
Use of composition containing bioactive collagen in cosmetics
1) The test method comprises the following steps: 36 volunteers (age 30-50 years) were randomly divided into 6 groups according to the parallel comparison principle, and the application examples 1-6 were used respectively, and the test volunteers used the corresponding skin care products after cleaning their faces in the morning and evening, and the service cycle was 8 weeks.
2) And (3) test results:
after 4 weeks of use, each group was evaluated for the degree of improvement in skin elasticity, and the results are shown in Table 5 below
TABLE 5 volunteer efficacy test evaluation results
The experimental result shows that the skin care product of the composition containing the bioactive collagen has a remarkable improvement effect on the elasticity of the skin compared with the skin care product without the bioactive collagen. Application examples 5 to 6 are products in which the bioactive collagen is added, but the improvement effect is significantly inferior to that of the composition in which the bioactive collagen is added.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (3)
1. A composition containing bioactive collagen peptide is characterized in that the composition is of a W/O/W type multiple emulsion structure, the D50 particle size of the W/O/W type multiple emulsion structure is 3-12 mu m, and the W/O/W type multiple emulsion structure consists of an internal water phase, an oil phase and an external water phase according to the mass ratio of (5-11) to (11-21) to (21-26);
the inner water phase consists of bioactive collagen peptide, cosolvent and accelerant according to the mass ratio of 1 (2-5) to (2-5);
the oil phase contains grease and an emulsifier I, the mass ratio of the emulsifier I to the grease is 1 (5-25), and the emulsifier I is a polyglycerol ester emulsifier;
the external water phase contains an emulsifier II, and the mass ratio of the emulsifier II to water is 1 (10-30);
the bioactive collagen peptide is I-type atelocollagen;
the cosolvent is one or more of 1, 3-butanediol, xylitol, lactitol and glycerol;
the accelerant is a polysaccharide component;
the grease is one or any mixture of hydrogenated polyisobutene, cetyl alcohol ethyl hexanoate, glycerol tri (ethyl hexanoate), octyl dodecanol myristate, diisostearyl malate and di (octyl dodecanol) malate; the emulsifier I is a mixture of any two emulsifiers in polyglycerol-3 decanoate, polyglycerol-4 oleate, polyglycerol-5 trioleate, polyglycerol-5 isostearate, polyglycerol-6 dioleate, polyglycerol-6 stearate, polyglycerol-6 isostearate and polyglycerol-6 pentaoleate in any mass ratio;
the emulsifier II is a mixture of any two emulsifiers of C8-C14 alkyl glucoside, C12-18 alkyl glucoside, sucrose pentahydroxystearate, sucrose tribehenate, sodium lauroyl lactylate, isostearyl lactate, distarch phosphate, oleyl polyether-5 phosphate and triolein polyether-8 phosphate in any mass ratio;
the composition containing the bioactive collagen peptide contains a high molecular polymer dispersing agent, wherein the high molecular polymer dispersing agent is selected from one or any mixture of an acrylic acid (ester) copolymer, an acrylic acid (ester) copolymer AMP salt, an ammonium acryloyldimethyl taurate/steareth-8 methacrylate copolymer, a steareth-10 allyl ether/acrylic acid (ester) copolymer and a beheneth-25 methacrylate cross-linked polymer, and the content of the high molecular polymer dispersing agent is 0.05-0.08% of the mass of the composition;
plant effective components are added into the internal water phase, and the mass ratio of the plant effective components to the bioactive collagen peptide, the cosolvent and the accelerant is (2-5) to 1 (2-5) to (2-5);
the plant effective components comprise the following raw materials in percentage by weight: 5-12% of witch hazel extract, 6-11% of tropaeolum extract, 2-4% of maca extract, 3-9% of almond extract, 2-12% of ginseng extract, 8-15% of aloe extract, 3-9% of polygala fallax hemsl extract, 2-8% of philippine flemingia root extract, 1-5% of dulcis japonicas extract and 0.8-2% of cerasus humilis kernel protein polypeptide.
2. The method of claim 1, wherein the composition comprises a bioactive collagen peptide, the method comprising the steps of:
(1) mixing and dissolving bioactive collagen peptide, plant effective components, cosolvent and accelerant to obtain an internal water phase; dissolving an emulsifier I in grease to obtain an oil phase; slowly adding the inner water phase into the oil phase, and homogenizing under ultrahigh pressure to obtain W/O emulsion with the particle size of 0.7-1.0 mu m; the pressure of the ultrahigh-pressure homogenization is 1100-1300 bar; the time is 15-30 min; the temperature is 0-25 ℃;
(2) dissolving an emulsifier II in water to obtain an external water phase; and (2) slowly adding the W/O emulsion and the high molecular polymer dispersing agent in the step (1) into the external water phase, and homogenizing under ultrahigh pressure to obtain the W/O/W type multiple emulsion.
3. The composition containing bioactive collagen peptides according to claim 1, wherein said composition is used in the preparation of a cosmetic.
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| CN114159360B (en) * | 2021-12-13 | 2023-08-22 | 武汉百思凯瑞生物科技有限公司 | Anti-blue light nanometer composition of bird's nest acid, preparation method and application thereof |
| CN114159335B (en) * | 2021-12-13 | 2023-08-22 | 武汉百思凯瑞生物科技有限公司 | Bird's nest acid moisturizing and repairing nano composition, preparation method thereof and cosmetic |
| CN114099365B (en) * | 2021-12-13 | 2023-08-22 | 武汉百思凯瑞生物科技有限公司 | Nidus Collocaliae acid whitening and skin brightening nanometer composition and preparation method and application thereof |
| CN114732774A (en) * | 2022-03-11 | 2022-07-12 | 广东丸美生物技术股份有限公司 | Composition containing traditional Chinese medicine extract and preparation method and application thereof |
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