CN109275508A - A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material - Google Patents

A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material Download PDF

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Publication number
CN109275508A
CN109275508A CN201811416787.4A CN201811416787A CN109275508A CN 109275508 A CN109275508 A CN 109275508A CN 201811416787 A CN201811416787 A CN 201811416787A CN 109275508 A CN109275508 A CN 109275508A
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white
parts
culture material
ganodermataceae dook
tea
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CN201811416787.4A
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陈检锋
王志远
陈华
尹梅
付利波
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Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
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Institute of Agricultural Environment and Resources of Yunnan Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00Cultivation of mushrooms
    • A01G18/20Culture media, e.g. compost

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Mushroom Cultivation (AREA)

Abstract

White Ganodermataceae Dook culture material and preparation method are prepared using tea place field trash the invention discloses a kind of, it is related to tea place green production and field of edible fungus culture, white Ganodermataceae Dook culture material includes following ingredient: tea tree branch 30-40%, Hairy vetch 30-40%, peanut or soybean stalks 20-30%, brown sugar 1-5%, pulverized limestone 1-5%, the white Ganodermataceae Dook culture material of the present invention is with tea place field trash (tea branch, Hairy vetch) for primary raw material, realize waste resource recovery, nutrient needed for compost is able to satisfy the growth of white Ganoderma lucidum mycelium, white ganoderma lucidum moral character good yield are high.

Description

A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material
Technical field
The present invention relates to tea place green production and field of edible fungus culture, and in particular to a kind of tea place field trash preparation The method of white Ganodermataceae Dook culture material.
Background technique
Bai Lingzhi, also known as beautiful sesame.It is mainly grown in relatively wet mountain forest, is distributed mainly on Yunnan Province, Tibet Autonomous Region And Changbaishan area, such as horse-hof shape, the big denumerable jin of person, it is born on pine tree and other coniferous trees.
Bai Lingzhi not only delicious flavour, and it is full of nutrition.Bai Lingzhi rich in wheat sterol containing angle, organic acid, D-MANNOSE, Glucosamine, polysaccharide, mannitol, fatty acid, amino acid, polypeptide, gucosamine, adenine, adenosine, uracil, lactone, Coumarin, triterpenes and a variety of enzymes and various trace elements, curing mainly has special efficacy to cardiovascular disease, moreover it is possible to dispelling wind and eliminating dampness, Hemostasis, resolving sputum, anticancer.
Meanwhile also there is white ganoderma lucidum improvement to sleep, and promotes toxin expelling, beautifying face and moistering lotion, improves the function such as immunity and nourishing and fit keeping function Effect.
Modern tea place usually in crops such as tea row interplant Hairy vetch, peanut, soya beans, will be timed repair every year It cuts, trims about 200kg/ mus of the tea branch amount got off;About 200-300kg/ mus of tea place intercrop Hairy vetch yield, peanut, Soybean stalks 100-200kg.Current main Land use systems are to cover on the spot, natural decomposition into field.But since tea branch is wooden Change degree is high, and decomposed slow, be deposited in tea place not only cannot achieve returning to the field for a long time, influences the normal farming operations in tea place instead.
If white Ganodermataceae Dook culture material can be made using tea branch, Hairy vetch, peanut and the soybean stalks in tea place, one Aspect enables tea place waste recycling to recycle, and on the other hand further decreases the aquaculture cost of white ganoderma lucidum, for The popularization cultivation of Bai Lingzhi all has important meaning.
Summary of the invention
(1) the technical issues of solving
In view of the deficiencies of the prior art, the present invention provides the sides that a kind of tea place field trash prepares white Ganodermataceae Dook culture material Tea place changing rejected material to useful resource is prepared into the compost specializing in white ganoderma lucidum and using by method, battalion needed for capable of providing white ganoderma lucidum growth Substance is supported, white ganoderma lucidum yield is effectively improved.
(2) technical solution
In order to achieve the above object, the present invention is achieved by the following technical programs:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after cutting tea tree secateurs using pulverizer, crosses 2 meshes and obtain tea tree branch powder, taking weight is tea tree branch The EM bacterium stoste of weight 1% is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, and stacking ferments a week, obtains tea tree branch Ferment powder;
(2) Hairy vetch, stalk are sufficiently crushed using pulverizer, crosses 2 meshes, obtained spare after mixed powder;
(3) it takes in brown sugar investment blender, the water of 5-10 times of brown sugar weight is added, stirring and dissolving is made additional to uniform Carbon source is with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 2- 3d obtains primary material;
(5) additional carbon is put into primary material and be sufficiently mixed, and pulverized limestone is added, slowly secondary plus water, adjust pH to 6.5-8.5 after standing 0.5h, is warming up to 40-45 DEG C of stirring evaporation, until the water content for adjusting compost is obtained to 60-70% Secondary material;
(6) secondary material is divided in polyethylene plastic bag, carries out atmospheric steam sterilizing, is cultivated after cooling up to white ganoderma lucidum Material.
Preferably, the white Ganodermataceae Dook culture material, the raw material composition including following parts by weight: tea tree branch 30-40 parts, light leaf 30-40 parts of Hairy Vetch, 20-30 parts of stalk, 1-5 parts of brown sugar, 1-5 parts of pulverized limestone.
Preferably, the white Ganodermataceae Dook culture material, the raw material composition including following parts by weight: 35 parts of tea tree branch, light leaf are purple Flower 35 parts of sweet potato, 25 parts of stalk, 3 parts of brown sugar, 2 parts of pulverized limestone.
Preferably, stalk described in step (2) is peanut straw, any one in soybean stalks.
Preferably, when once adding water in step (4), the moisture of primary material is controlled in 50-60%.
Preferably, it is rapidly heated in half an hour after to the sterilizing of polyethylene plastic bag atmospheric steam to 100 in step (6) DEG C, and room temperature is cooled to after holding 10h at such a temperature.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
(3) beneficial effect
The present invention provides a kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, have below beneficial to effect Fruit:
Firstly, being unable to fully resource utilization for tea place field trash in the prior art, tea place production burden is caused The problem of, tea place field trash is collected, handles by the present invention, and white ganoderma lucidum culture is made by specific preparation method Material effectively realizes the changing rejected material to useful resource in tea place field, environmentally protective.
Secondly, increasing additional carbon, pulverized limestone on the basis of the field trash of tea place, while adjusting compost pH, making The growing environment that white Ganodermataceae Dook culture material is more in line with white ganoderma lucidum is obtained, while nutriment is able to satisfy needed for the growth of white Ganoderma lucidum mycelium and supports Point, guarantee that white ganoderma lucidum moral character is good, yield is high.
Specific embodiment
In order to make the object, technical scheme and advantages of the embodiment of the invention clearer, below in conjunction with the embodiment of the present invention, Technical scheme in the embodiment of the invention is clearly and completely described, it is clear that described embodiment is the present invention one Divide embodiment, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not making Every other embodiment obtained, shall fall within the protection scope of the present invention under the premise of creative work.
Embodiment 1:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after taking the 30 parts of shearings of tea tree branch according to parts by weight using pulverizer, crosses 2 meshes and obtain tea tree branch powder End, taking weight is the EM bacterium stoste of tea tree branch weight 1%, is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, stacking fermentation one Obtain tea tree branch fermentation powder in a week;
(2) take according to parts by weight 40 parts of Hairy vetch, 20 parts of soybean stalks sufficiently crushed using pulverizer, cross 2 mesh Sieve obtains spare after mixed powder;
(3) it takes according to parts by weight in 5 parts of investment blenders of brown sugar, is added 5 times of brown sugar weight of water, stirring and dissolving is to equal It is even, additional carbon is made with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 3d, Primary material is obtained, controls the moisture of primary material 50%.
(5) additional carbon is put into primary material and is sufficiently mixed, and take 1 part of addition mixing of pulverized limestone according to parts by weight, Slowly secondary plus water adjusts pH to 6.5, after standing 0.5h, 45 DEG C of stirring evaporations is warming up to, until adjusting the water content of compost To 70%, secondary material is obtained;
(6) by secondary material be divided in polyethylene plastic bag, carry out atmospheric steam sterilizing, be rapidly heated in half an hour to 100 DEG C, and room temperature is cooled to after holding 10h at such a temperature.Up to white Ganodermataceae Dook culture material after cooling.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
Embodiment 2:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after taking the 40 parts of shearings of tea tree branch according to parts by weight using pulverizer, crosses 2 meshes and obtain tea tree branch powder End, taking weight is the EM bacterium stoste of tea tree branch weight 1%, is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, stacking fermentation one Obtain tea tree branch fermentation powder in a week;
(2) take according to parts by weight 30 parts of Hairy vetch, 30 parts of peanut straw sufficiently crushed using pulverizer, cross 2 mesh Sieve obtains spare after mixed powder;
(3) it takes according to parts by weight in 1 part of investment blender of brown sugar, 10 times of brown sugar weight of water is added, stirring and dissolving is extremely Uniformly, additional carbon is made with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 2d, Primary material is obtained, controls the moisture of primary material 60%.
(5) additional carbon is put into primary material and is sufficiently mixed, and take 5 parts of addition mixing of pulverized limestone according to parts by weight, Slowly secondary plus water adjusts pH to 8.5, after standing 0.5h, 45 DEG C of stirring evaporations is warming up to, until adjusting the water content of compost To 60%, secondary material is obtained;
(6) by secondary material be divided in polyethylene plastic bag, carry out atmospheric steam sterilizing, be rapidly heated in half an hour to 100 DEG C, and room temperature is cooled to after holding 10h at such a temperature.Up to white Ganodermataceae Dook culture material after cooling.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
Embodiment 3:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after taking the 35 parts of shearings of tea tree branch according to parts by weight using pulverizer, crosses 2 meshes and obtain tea tree branch powder End, taking weight is the EM bacterium stoste of tea tree branch weight 1%, is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, stacking fermentation one Obtain tea tree branch fermentation powder in a week;
(2) take according to parts by weight 35 parts of Hairy vetch, 25 parts of soybean stalks sufficiently crushed using pulverizer, cross 2 mesh Sieve obtains spare after mixed powder;
(3) it takes according to parts by weight in 3 parts of investment blenders of brown sugar, is added 6 times of brown sugar weight of water, stirring and dissolving is to equal It is even, additional carbon is made with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 2.5d obtains primary material, controls the moisture of primary material 55%.
(5) additional carbon is put into primary material and is sufficiently mixed, and take 2 parts of addition mixing of pulverized limestone according to parts by weight, Slowly secondary plus water adjusts pH to 7, after standing 0.5h, is warming up to 43 DEG C of stirrings evaporations, until the water content of adjusting compost to 66%, obtain secondary material;
(6) by secondary material be divided in polyethylene plastic bag, carry out atmospheric steam sterilizing, be rapidly heated in half an hour to 100 DEG C, and room temperature is cooled to after holding 10h at such a temperature.Up to white Ganodermataceae Dook culture material after cooling.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
Embodiment 4:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after taking the 33 parts of shearings of tea tree branch according to parts by weight using pulverizer, crosses 2 meshes and obtain tea tree branch powder End, taking weight is the EM bacterium stoste of tea tree branch weight 1%, is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, stacking fermentation one Obtain tea tree branch fermentation powder in a week;
(2) take according to parts by weight 36 parts of Hairy vetch, 25 parts of soybean stalks sufficiently crushed using pulverizer, cross 2 mesh Sieve obtains spare after mixed powder;
(3) it takes according to parts by weight in 2 parts of investment blenders of brown sugar, is added 7 times of brown sugar weight of water, stirring and dissolving is to equal It is even, additional carbon is made with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 2d, Primary material is obtained, controls the moisture of primary material 54%.
(5) additional carbon is put into primary material and is sufficiently mixed, and taken 2.5 parts of pulverized limestone to be added according to parts by weight and mix It closes, slowly secondary plus water adjusts pH to 7.6, after standing 0.5h, 42 DEG C of stirring evaporations is warming up to, until adjusting containing for compost Water obtains secondary material to 68%;
(6) by secondary material be divided in polyethylene plastic bag, carry out atmospheric steam sterilizing, be rapidly heated in half an hour to 100 DEG C, and room temperature is cooled to after holding 10h at such a temperature.Up to white Ganodermataceae Dook culture material after cooling.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
Embodiment 5:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after taking the 37 parts of shearings of tea tree branch according to parts by weight using pulverizer, crosses 2 meshes and obtain tea tree branch powder End, taking weight is the EM bacterium stoste of tea tree branch weight 1%, is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, stacking fermentation one Obtain tea tree branch fermentation powder in a week;
(2) take according to parts by weight 32 parts of Hairy vetch, 26 parts of peanut straw sufficiently crushed using pulverizer, cross 2 mesh Sieve obtains spare after mixed powder;
(3) it takes according to parts by weight in 3 parts of investment blenders of brown sugar, is added 7 times of brown sugar weight of water, stirring and dissolving is to equal It is even, additional carbon is made with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 3d, Primary material is obtained, controls the moisture of primary material 52%.
(5) additional carbon is put into primary material and is sufficiently mixed, and take 4 parts of addition mixing of pulverized limestone according to parts by weight, Slowly secondary plus water adjusts pH to 8, after standing 0.5h, is warming up to 42 DEG C of stirrings evaporations, until the water content of adjusting compost to 66%, obtain secondary material;
(6) by secondary material be divided in polyethylene plastic bag, carry out atmospheric steam sterilizing, be rapidly heated in half an hour to 100 DEG C, and room temperature is cooled to after holding 10h at such a temperature.Up to white Ganodermataceae Dook culture material after cooling.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
Embodiment 6:
A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, comprising the following steps:
(1) it is crushed after taking the 35 parts of shearings of tea tree branch according to parts by weight using pulverizer, crosses 2 meshes and obtain tea tree branch powder End, taking weight is the EM bacterium stoste of tea tree branch weight 1%, is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, stacking fermentation one Obtain tea tree branch fermentation powder in a week;
(2) take according to parts by weight 32 parts of Hairy vetch, 29 parts of soybean stalks sufficiently crushed using pulverizer, cross 2 mesh Sieve obtains spare after mixed powder;
(3) it takes according to parts by weight in 4 parts of investment blenders of brown sugar, is added 6 times of brown sugar weight of water, stirring and dissolving is to equal It is even, additional carbon is made with spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 2d, Primary material is obtained, controls the moisture of primary material 60%.
(5) additional carbon is put into primary material and is sufficiently mixed, and taken 4.5 parts of pulverized limestone to be added according to parts by weight and mix It closes, slowly secondary plus water adjusts pH to 8.2, after standing 0.5h, 41 DEG C of stirring evaporations is warming up to, until adjusting containing for compost Water obtains secondary material to 66%;
(6) by secondary material be divided in polyethylene plastic bag, carry out atmospheric steam sterilizing, be rapidly heated in half an hour to 100 DEG C, and room temperature is cooled to after holding 10h at such a temperature.Up to white Ganodermataceae Dook culture material after cooling.
The application of above-mentioned white Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum Bacteria stick.
Beneficial effect in order to further illustrate the present invention selects the white Ganodermataceae Dook culture material of 1-4 of embodiment of the present invention preparation As experiment case study 1-4, while directly, the white Ganodermataceae Dook culture material (sawdust) of purchase in the market is as comparison case, in the same terms It is lower to carry out white ganoderma lucidum culture, and measure its biological transformation ratio and white ganoderma lucidum is averaged single bag of yield, while experimental result being recorded in In following table.
The white ganoderma lucidum morphological feature of the 1 white Ganodermataceae Dook culture material of difference of table and Yield comparison
As known to table 1: being able to satisfy the growth of white ganoderma lucidum by white Ganodermataceae Dook culture material prepared by the present invention, white Ganodermataceae Dook culture material is raw Object conversion ratio is got well than comparing case, and white ganoderma lucidum yield is higher by 12% than comparison case processing yield.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment Intrinsic element.In the absence of more restrictions, including the element that sentence "including a ..." limits, it is not excluded that There is also other identical elements in the process, method, article or apparatus that includes the element.
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations, although with reference to the foregoing embodiments Invention is explained in detail, those skilled in the art should understand that: it still can be to aforementioned each implementation Technical solution documented by example is modified or equivalent replacement of some of the technical features;And these modification or Replacement, the spirit and scope for technical solution of various embodiments of the present invention that it does not separate the essence of the corresponding technical solution.

Claims (7)

1. a kind of method that tea place field trash prepares white Ganodermataceae Dook culture material, which comprises the following steps:
(1) it is crushed after cutting tea tree secateurs using pulverizer, crosses 2 meshes and obtain tea tree branch powder, taking weight is tea tree branch weight 1% EM bacterium stoste is mixed thoroughly after being diluted with water 10 times with tea tree branch powder, and stacking ferments a week, obtains tea tree branch fermentation Powder;
(2) Hairy vetch, stalk are sufficiently crushed using pulverizer, crosses 2 meshes, obtained spare after mixed powder;
(3) it takes in brown sugar investment blender, the water of 5-10 times of brown sugar weight, stirring and dissolving to uniform, obtained additional carbon is added With spare;
(4) it by tea tree branch fermentation powder, mixed powder investment reaction kettle, is uniformly mixed, once plus water is prewetted 2-3d, is obtained To primary material;
(5) additional carbon is put into primary material and is sufficiently mixed, and pulverized limestone is added, slowly secondary plus water adjusts pH to 6.5- 8.5, after standing 0.5h, it is warming up to 40-45 DEG C of stirring evaporation, until the water content for adjusting compost obtains secondary to 60-70% Material;
(6) secondary material is divided in polyethylene plastic bag, atmospheric steam sterilizing is carried out, up to white Ganodermataceae Dook culture material after cooling.
2. the method that tea place field trash as described in claim 1 prepares white Ganodermataceae Dook culture material, which is characterized in that described white Ganodermataceae Dook culture material, the raw material composition including following parts by weight: tea tree branch 30-40 parts, 30-40 parts of Hairy vetch, stalk 20- 30 parts, 1-5 parts of brown sugar, 1-5 parts of pulverized limestone.
3. the method that tea place field trash as claimed in claim 2 prepares white Ganodermataceae Dook culture material, which is characterized in that described white Ganodermataceae Dook culture material, the raw material composition including following parts by weight: tea tree branch 35 parts, 35 parts of Hairy vetch, 25 parts of stalk, brown sugar 3 parts, 2 parts of pulverized limestone.
4. the method that tea place field trash as described in claim 1 prepares white Ganodermataceae Dook culture material, which is characterized in that step (2) stalk described in is peanut straw, any one in soybean stalks.
5. the method that tea place field trash as described in claim 1 prepares white Ganodermataceae Dook culture material, which is characterized in that step (4) when once adding water in, the moisture of primary material is controlled in 50-60%.
6. the method that tea place field trash as described in claim 1 prepares white Ganodermataceae Dook culture material, which is characterized in that step (6) it in after to the sterilizing of polyethylene plastic bag atmospheric steam, is rapidly heated in half an hour to 100 DEG C, and keep at such a temperature After 10h, it is cooled to room temperature.
7. the method that tea place field trash as described in claim 1 prepares white Ganodermataceae Dook culture material, which is characterized in that described white The application of Ganodermataceae Dook culture material: white Ganodermataceae Dook culture material after cooling is inoculated with white Ganoderma lucidum mycelium and obtains white ganoderma lucidum bacteria stick.
CN201811416787.4A 2018-11-26 2018-11-26 A kind of method that tea place field trash prepares white Ganodermataceae Dook culture material Pending CN109275508A (en)

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