CN109251843A - It is split type to revolve polycyclic type mycorhiza locellus culture and processing unit - Google Patents
It is split type to revolve polycyclic type mycorhiza locellus culture and processing unit Download PDFInfo
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Abstract
Split type to revolve polycyclic type mycorhiza locellus culture and processing unit, the invention belongs to arbuscular mycorrhizal fungi field, it is in order to solve the problems, such as that existing locellus culture apparatus is difficult to timing sampling.The locellus culture and processing unit include phytotron, hyphal development room, experiment control cylinder and pedestal, motor is provided in the pedestal of pedestal, rotation is driven by motor in experiment control cylinder, phytotron is arranged in experiment control cylinder, multiple hyphal development rooms are arranged along the circumferential direction of phytotron, hyphal development room is plugged on the cylinder bottom upper surface of experiment control cylinder, there are fluid courses between the cylinder bottom of the bottom wall and experiment control cylinder of phytotron and hyphal development room, each hyphal development room, which is closed between the room side wall of phytotron and the outer surface of phytotron, forms buffer area.The present invention uses split type hyphal development room, and hyphal development room is plugged on the cylinder bottom of experiment control cylinder, convenient for individually taking out single bacterium room development room in different time sections.
Description
Technical field
The invention belongs to arbuscular mycorrhizal fungi fields, and in particular to the outer mycelia Synergistic Mechanisms of a kind of research root system, root
And the device of hypha separation culture and disposition.
Background technique
Arbuscular mycorrhizal fungi is closely linked together by underground mycelia network and plant, and mycorrhizal fungi passes through mycelia net
Network obtains the organic nutrient substances such as necessary carbohydrate out of plant to supply the growth and development of itself, and plant is then
It can therefrom obtain by inorganic matters such as the mineral nutrient element of mycorrhizal fungi absorption and moisture.Arbuscular mycorrhizal fungi infects host
Afterwards, clump branch on the one hand can be formed to root system of plant growth inside, on the other hand can root external expansion, form complicated mycelia net
The outer mycelia permafro research of network, root system and root is the important content of mycorhiza effect study, with the proviso that the purifying of mycelia, spore
Isolation and culture.It is developed since mycorrhizal fungi can not be detached from the host's complete independently complete period history of life, locellus culture systems are therefore
And give birth to, it is isolated by locellus, realizes that mycelia is isolated with the space of root system and the purifying of mycelia obtains.
Traditional locellus culture apparatus is primarily present following common problem:
1, mycelia room and phytotron can not completely cut through the phase of environmental factor in two regions there is no completely isolated
It mutually influences, the certain media in phytotron can enter mycelia room, be unfavorable for studying the outer mycelia of root in mycorhiza effect
Although contribution, nylon wire are able to achieve the physical isolation of mycelia root system, it can be difficult to realizing the element sepatations such as secretion；
2, root system of plant has a physical feature of radiation growth, and traditional square locellus culture studies are there are edge effect,
Mycelia outside root system and root can not be separated from multiple directions, it is difficult to embody the function of the outer mycelia of root；
It 3, is fixed device, the timing sampling being unable to complete in history of life research can not eliminate culture environment heterogeneity
It influences, and experiment can not synchronize realization processing and repeat, no spore separation system, it is difficult to realize industrial application.
Summary of the invention
The purpose of the present invention is to solve existing locellus culture apparatus to be difficult to timing sampling, the bacterium in locellus culture apparatus
Environmental factor in cabinet and plant growth room influences each other, and root system and the research of the outer mycelia mechanism of action of root is caused to be affected
The problem of, and provide and a kind of split type revolve polycyclic type mycorhiza locellus culture and processing unit.
The present invention is split type to revolve polycyclic type mycorhiza locellus culture and processing unit includes phytotron, hyphal development
Room, experiment control cylinder and pedestal, the center point of pedestal offer motor room, and motor is placed in motor room, the shaft of motor and bottom
The upper surface of seat is perpendicular, is provided with the sump pit of annular in the upper surface of pedestal, sump pit is connected with liquid outlet channel, in pedestal
The circumferential direction of upper surface is evenly arranged with multiple back-up roller wheels, and the cylinder bottom surface of experiment control cylinder is in contact with back-up roller wheel, experiment control
Rotation is driven by motor in cylinder processed；
Phytotron is cylindrical shape, is uniformly provided with multiple through-holes, the bottom of phytotron on the barrel of phytotron
Multiple micropores are uniformly provided on wall, application cylinder supporting leg is arranged in the cylinder bottom of phytotron, and phytotron is arranged in experiment control
The cylinder bottom the center point of cylinder, the room bottom setting development room supporting leg of hyphal development room, circumferential direction of multiple hyphal development rooms along plant growth
Setting, hyphal development room are plugged on the cylinder bottom upper surface of experiment control cylinder, and the room side of phytotron is closed in hyphal development room
It is uniformly provided with multiple through-holes on wall, is uniformly provided with multiple micropores on the bottom wall of hyphal development room, the bottom wall table in hyphal development room
Face paste has one-way membrane, and the one-way membrane is connected from bottom to top；
There are fluid course, each bacterium between the cylinder bottom of the bottom wall and experiment control cylinder of phytotron and hyphal development room
Silk development room, which is closed between the room side wall of phytotron and the outer surface of phytotron, forms buffer area, the width of buffer area
D is 1.5cm~2.5cm, is provided with cricoid leakage channel, the leakage mouth in leakage channel on the cylinder bottom of experiment control cylinder in buffer area
It extends in sump pit.
The present invention is split type revolve polycyclic type mycorhiza locellus culture and processing unit include it is following the utility model has the advantages that
The present invention uses split type hyphal development room, and hyphal development room is plugged on the cylinder bottom of experiment control cylinder, and being convenient for will
Single bacterium room development room is individually taken out in different time sections, is conducive to the physiological ecological function of continuous observational study mycelia, real
The continuous Journal of Sex Research of the same cogeneration system root outer mycelia physiological ecological function and structure is showed.
The present invention takes circular experimental control room to penetrate through the structure of entire locellus bottom of device, the bottom wall in hyphal development room
On be pasted with one-way membrane, realize phytotron and hyphal development room it is identical stress and nutritional condition under element separate,
The quick transmission for being conducive to the whole control and nutriment of stress conditions, has prevented interfering with each other for the two room environmental factors.
Reasonable disposition phytotron of the present invention, hyphal development room, experiment control room polycyclic shape space layout, promote
The growth of mycelia and the uniform transport of nutriment, phytotron are cylindrical structure, and hyphal development room is along phytotron
Circumferential direction be uniformly arranged, eliminate the influence of edge effect, enable root system and mycelia to obtain good growth and development, and make
It obtains most root system and hypha separation comes.
The present invention rotatably facilitates the homoepitaxial of plant using locellus device, avoids illumination, moisture pair
The influence of plant growth and hyphal development, the upper environmental heterogeneity that reduces tests mycelia permafro outside root system and root to greatest extent
The influence of research improves the accuracy and repeatability of research experiment.And spore separation system-filter disc under culture apparatus
The separation and collection of spore can be achieved.
Detailed description of the invention
Fig. 1 is the split type structural schematic diagram for revolving polycyclic type mycorhiza locellus device of the present invention；
Fig. 2 is the structural schematic diagram of the cylinder bottom groove of experiment control cylinder；
Fig. 3 is the top view of hyphal development room described in specific embodiment two；
Fig. 4 is the structural schematic diagram of the development room supporting leg of hyphal development room；
Fig. 5 is the structural schematic diagram that phytotron is inserted into groove in specific embodiment six；
Fig. 6 is the structural schematic diagram of filter disc below hyphal development room described in specific embodiment seven；
Fig. 7 is the structural schematic diagram of filter disc socket below phytotron described in specific embodiment seven.
Specific embodiment
Specific embodiment 1: split type polycyclic type mycorhiza locellus culture and the processing unit of revolving of present embodiment is including planting
Object growth room 1, hyphal development room 2, experiment control cylinder 3 and pedestal 4, the center point of pedestal 4 offer motor room, and motor 5 is placed in
In motor room, the shaft of motor 5 and the upper surface of pedestal 4 are perpendicular, are provided with the sump pit 4-1 of annular in the upper surface of pedestal 4,
Sump pit 4-1 is connected with liquid outlet channel 4-2, and the circumferential direction in 4 upper surface of pedestal is evenly arranged with multiple back-up roller wheel 4-3, real
The cylinder bottom surface for testing application cylinder 3 is in contact with back-up roller wheel 4-3, and experiment control cylinder 3 is driven by motor 5 to be rotated；
Phytotron 1 is cylindrical shape, is uniformly provided with multiple through-holes on the barrel of phytotron 1, phytotron 1
Multiple micropores are uniformly provided on bottom wall, application cylinder supporting leg 1-1 is arranged in the cylinder bottom of phytotron 1, and phytotron 1 is arranged in reality
Test the cylinder bottom the center point of application cylinder 3, the room bottom setting development room supporting leg 2-1 of hyphal development room 2, multiple hyphal development rooms 2 are along plant
The circumferential setting of object growth room 1, hyphal development room 2 are plugged on the cylinder bottom upper surface of experiment control cylinder 3, and hyphal development room 2 is faced
Multiple through-holes are uniformly provided on the room side wall 2-2 of nearly phytotron 1, are uniformly provided on the bottom wall of hyphal development room 2 multiple micro-
Hole, the bottom wall surface in hyphal development room 2 are pasted with one-way membrane, and the one-way membrane is connected from bottom to top；
There are fluid courses between the cylinder bottom of the bottom wall and experiment control cylinder 3 of phytotron 1 and hyphal development room 2, often
A hyphal development room 2, which is closed between the room side wall 2-2 of phytotron 1 and the outer surface of phytotron 1, forms buffer area, delays
The width d for rushing area is 1.5cm~2.5cm, is provided with cricoid leakage channel 3-1 on the cylinder bottom of experiment control cylinder 3 in buffer area, leaks
The leakage mouth of liquid channel 3-1 extends in sump pit 4-1.
Specific embodiment 2: the present embodiment is different from the first embodiment in that the cross section of hyphal development room 2
Shape is sector.
The cross section of present embodiment hyphal development room as shown in figure 3, multiple hyphal development rooms 2 along phytotron 1
Circumferential setting, multiple hyphal development rooms 2 surround circular ring shape.
Specific embodiment 3: the present embodiment is different from the first and the second embodiment in that shell 6 is arranged in pedestal 4
Upper surface on, the axial direction of shell 6 and the upper surface of pedestal 4 are perpendicular, and shell 6 is set in the outside of experiment control cylinder 3.
Phytotron, hyphal development room, experiment control cylinder are arranged inside the housing by present embodiment, form closing knot
Structure.Present embodiment phytotron, hyphal development room, experiment control cylinder and shell material be glass plate, reality can be made
It tests personnel and quickly grasps situation inside equipment, influence of the outside environmental elements to system can also be reduced, make internal continuous protect
Keep steady fixed environment.
Specific embodiment 4: present embodiment is covered from top cover 7 unlike specific embodiment three is located at the spacious of shell 6
In opening's edge.
Light compensating lamp is additionally provided in present embodiment top cover.
Specific embodiment 5: in phytotron unlike one of present embodiment and specific embodiment one to four
1 and the top of hyphal development room 2 be provided with water distributor 8.
Present embodiment had both been able to achieve the locellus culture of earth culture by the different controls of water distributor and experiment control room, can also
To realize the locellus culture of half water planting.
Specific embodiment 6: in experiment control cylinder unlike one of present embodiment and specific embodiment one to five
3 cylinder bottom is provided with ring groove 9-1, and the application cylinder supporting leg 1-1 of phytotron 1 is inserted into ring groove 9-1, in experiment control
The cylinder bottom of cylinder 3 is provided with a plurality of radial groove 9-2, and the development room supporting leg 2-1 of hyphal development room 2 is inserted into radial groove 9-2.
Specific embodiment 7: in hyphal development room unlike one of present embodiment and specific embodiment one to six
It is provided with the first filter disc 10 below 2 bottom wall, the second filter disc 11, hyphal development are provided with below the bottom wall of phytotron 1
First filter disc 10 of 2 lower section of room is plugged on development room supporting leg 2-1；Second filter disc 11 of 1 lower section of phytotron is second
Multiple positioning protrusion 11-1 are evenly arranged on the disk body circumferencial direction of filter disc 11, the inner circle circumferential direction of application cylinder supporting leg 1-1 is set
Convex is equipped with along 1-2, is provided with the recess with the positioning protrusion 11-1 of the second filter disc 11 cooperation along 1-2 in convex, second
Filter disc 11 is overlapped on convex along 1-2.
Filter disc described in present embodiment is made of upper, middle and lower three-layer metal strainer, and the mesh aperture of upper layer metal screen is
0.88mm, the mesh aperture of middle layer metal strainer are 0.25mm, and the mesh aperture of lower metal strainer is 0.055mm, filter disc 10
Integral thickness be 2-3cm.The spore to get off is washed away in lower screen access phytotron or hyphal development room, to realize production
Industry application.
The first filter disc 10 below present embodiment hyphal development room is plugged between two development room supporting leg 2-1, structure
Figure is as shown in fig. 6, in the slot 2-2 of the grafting protrusion 10-1 insertion development room supporting leg 2-1 of 10 two sides of the first filter disc.Plant growth
Second filter disc 11 of 1 lower section of room is that multiple positioning protrusion 11-1 are evenly arranged on disk body circumferencial direction, application cylinder supporting leg 1-1
Circumferencial direction be provided with convex along 1-2, be provided with along 1-2 in convex and the positioning protrusion 11-1 of the second filter disc 11 cooperate
Recess, recess of the positioning protrusion 11-1 alignment convex on 1-2 of the second filter disc 11, then rotating the second filter disc 11 makes the
Two filter discs 11 are overlapped on convex along 1-2.
Specific embodiment 8: phytotron 1 unlike one of present embodiment and specific embodiment one to seven
Identical with the height of hyphal development room 2, the volumetric ratio of phytotron 1 and all hyphal development rooms 2 is 1~3:4~6.
Specific embodiment 9: phytotron 1 unlike one of present embodiment and specific embodiment one to eight
Barrel and hyphal development room 2 room side wall 2-2 on through-hole aperture be 0.5~1cm.
Specific embodiment 10: phytotron 1 unlike one of present embodiment and specific embodiment one to eight
The aperture of the micropore on 2 bottom wall of micropore and hyphal development room on bottom wall is 1~2mm.
Specific embodiment 11: phytotron unlike one of present embodiment and specific embodiment one to ten
1 and hyphal development room 2 bottom wall and experiment control cylinder 3 cylinder bottom between there are fluid course, the height h of the fluid course is
3~5cm.
Embodiment: the present embodiment is split type, and to revolve polycyclic type mycorhiza locellus device include phytotron 1, hyphal development room
2, experiment control cylinder 3 and pedestal 4, the center point of pedestal 4 offer motor room, and motor 5 is placed in motor room, the shaft of motor 5
It is perpendicular with the upper surface of pedestal 4, the sump pit 4-1 of annular, sump pit 4-1 and liquid outlet channel are provided in the upper surface of pedestal 4
4-2 is connected, liquid outlet channel 4-2 4 introversion of pedestal obliquely, the circumferential direction in 4 upper surface of pedestal is evenly arranged with 6 back-up rollers
4-3 is taken turns, the cylinder bottom surface of experiment control cylinder 3 is in contact with back-up roller wheel 4-3, and experiment control cylinder 3 is driven by motor 5 to be rotated；
Phytotron 1 is cylindrical shape, and the through-hole of multiple diameter 5mm, plant are uniformly provided on the barrel of phytotron 1
Multiple diameter 2mm micropores are uniformly provided on the bottom wall of growth room 1, the cylinder bottom of phytotron 1 is arranged application cylinder supporting leg 1-1, plants
The application cylinder supporting leg 1-1 of object growth room 1 is plugged on the annular groove 9-1 at 3 bottoms of experiment control cylinder, is arranged in annular groove 9-1
There is positioning protrusion 9-3, the room bottom setting development room supporting leg 2-1 of hyphal development room 2,6 hyphal development rooms 2 are along phytotron 1
Circumferential setting, the development room supporting leg 2-1 of hyphal development room 2 is plugged in the radial groove 9-2 at 3 bottoms of experiment control cylinder, bacterium
Silk development room 2, which is closed on the room side wall 2-2 of phytotron 1, the through-hole for being provided with multiple diameter 5mm, hyphal development room 2
The micropore that multiple apertures are 2mm is uniformly provided on bottom wall, the bottom wall upper surface in hyphal development room 2 is pasted with one-way membrane, described
One-way membrane be connected from bottom to top；
There are fluid course, liquid between the cylinder bottom of the bottom wall and experiment control cylinder 3 of phytotron 1 and hyphal development room 2
The height h of circulation road is 4cm, each hyphal development room 2 close on phytotron 1 room side wall 2-2 and phytotron 1 it is outer
Buffer area is formed between surface, the width d of buffer area is 2cm, is provided with cricoid leakage on the cylinder bottom of experiment control cylinder 3 in buffer area
The leakage mouth of liquid channel 3-1, leakage channel 3-1 extend in sump pit 4-1；
The top of phytotron 1 and hyphal development room 2 is provided with water distributor 8.
The present embodiment is provided with the first filter disc 10 below the bottom wall of hyphal development room 2, under the bottom wall of phytotron 1
Side is provided with the second filter disc 11, and the first filter disc 10 of 2 lower section of hyphal development room is plugged on development room supporting leg 2-1；Plant growth
Second filter disc 11 of 1 lower section of room is that multiple positioning protrusion 11-1 are evenly arranged on the disk body circumferencial direction of the second filter disc 11,
The inner circle circumferential direction of application cylinder supporting leg 1-1 is provided with convex along 1-2, is provided with along 1-2 and the second filter disc 11 in convex
The recess of positioning protrusion 11-1 cooperation, the second filter disc 11 are overlapped on convex along 1-2.
The filter disc with a thickness of 2cm, filter disc is in contact with phytotron or hyphal development room bottom wall lower surface.
The internal diameter of the present embodiment shell 6 is 40cm, and the cylinder internal diameter of phytotron 1 is 10cm, control experiment control cylinder 3
Revolving speed is 10r/d (day).
Phytotron 1 and hyphal development room 2 are made of glass plate in the present embodiment, the inner tube wall patch of phytotron 1
The nylon wire for being 27um with mesh aperture.
Application Example 1: the sterilizing vermiculite of 2/3 volume being added to phytotron, and 2/3 volume is added in hyphal development room
Bead (0.8~1.2mm of partial size) is added Glomus intraradices according to the 3% of sterilizing vermiculite mass percentage and mixes, by reed
Reed seed is sown into phytotron, stress nutrient solution is added in experiment control cylinder, while keeping plant raw by water distributor watering
Half water planting condition is kept in long room and hyphal development, is successively taken within the 10th, 20,30,40,50,60 day after reed reached for 4 leaf phases
Hyphal development room takes out and observes mycelial structure under the microscope out.
Application Example 2: the sterilized soil of 2/3 volume being added to phytotron and hyphal development room, according to sterilizing soil property
3% addition Glomus intraradices of amount percentage composition simultaneously mix, and reed seed are sown into phytotron, in experiment control cylinder not
Stress nutrient solution is added, makes to keep moisture state in phytotron and hyphal development by water distributor watering, reaches 4 in reed
Hyphal development room is successively taken out within the 10th, 20,30,40,50,60 day after the leaf phase to take out and measure phytotron and hyphal development
The indices of indoor rhizosphere soil.
Claims (10)
1. split type revolve polycyclic type mycorhiza locellus culture and processing unit, it is characterised in that this is split type to revolve polycyclic type mycorhiza
Locellus culture and processing unit include phytotron (1), hyphal development room (2), experiment control cylinder (3) and pedestal (4), pedestal
(4) the center point offers motor room, and motor (5) is placed in motor room, the shaft and the upper surface phase of pedestal (4) of motor (5)
Vertically, the sump pit (4-1) of annular is provided in the upper surface of pedestal (4), sump pit (4-1) is connected with liquid outlet channel (4-2),
Circumferential direction in pedestal (4) upper surface is evenly arranged with multiple back-up roller wheels (4-3), the cylinder bottom surface of experiment control cylinder (3) and support
Idler wheel (4-3) is in contact, and experiment control cylinder (3) is driven by motor (5) to be rotated；
Phytotron (1) is cylindrical shape, is uniformly provided with multiple through-holes, phytotron (1) on the barrel of phytotron (1)
Bottom wall on be uniformly provided with multiple micropores, application cylinder supporting leg (1-1) is arranged in the cylinder bottom of phytotron (1), phytotron (1)
Cylinder bottom the center point in experiment control cylinder (3), room bottom setting development room supporting leg (2-1) of hyphal development room (2), multiple bacterium are set
Along the circumferential direction setting of phytotron (1), hyphal development room (2) are plugged on the cylinder bottom of experiment control cylinder (3) for silk development room (2)
On surface, hyphal development room (2), which are closed on the room side wall (2-2) of phytotron (1), is uniformly provided with multiple through-holes, hyphal development
Multiple micropores are uniformly provided on the bottom wall of room (2), the bottom wall surface in hyphal development room (2) is pasted with one-way membrane, the list
It is connected from bottom to top to film；
There are fluid course between the cylinder bottom of the bottom wall and experiment control cylinder (3) of phytotron (1) and hyphal development room (2),
Each hyphal development room (2) closes on shape between the room side wall (2-2) of phytotron (1) and the outer surface of phytotron (1)
At buffer area, the width d of buffer area is 1.5cm~2.5cm, is provided on the cylinder bottom of experiment control cylinder (3) in buffer area cricoid
Leakage channel (3-1), the leakage mouth of leakage channel (3-1) extend in sump pit (4-1).
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 2. according to claim 1, it is characterised in that bacterium
The cross-sectional shape of silk development room (2) is sector.
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 3. according to claim 1, it is characterised in that outer
Shell (6) is arranged on the upper surface of pedestal (4), and the axial direction of shell (6) and the upper surface of pedestal (4) are perpendicular, and shell (6) is arranged
In the outside of experiment control cylinder (3).
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 4. according to claim 3, it is characterised in that top
Lid (7) lid is located at the opening of shell (6) along upper.
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 5. according to claim 1, it is characterised in that
The top of phytotron (1) and hyphal development room (2) is provided with water distributor (8).
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 6. according to claim 1, it is characterised in that
The cylinder bottom of experiment control cylinder (3) is provided with ring groove (9-1), and application cylinder supporting leg (1-1) the insertion annulus of phytotron (1) is recessed
In slot (9-1), it is provided with a plurality of radial groove (9-2) at the cylinder bottom of experiment control cylinder (3), the development room branch of hyphal development room (2)
Leg (2-1) is inserted into radial groove (9-2).
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 7. according to claim 1, it is characterised in that
The first filter disc (10) are provided with below the bottom wall of hyphal development room (2), are provided with second below the bottom wall of phytotron (1)
Filter disc (11)；The first filter disc (10) below hyphal development room (2) is plugged in development room supporting leg (2-1)；Phytotron (1)
The second filter disc (11) of lower section is that multiple positioning protrusion (11- are evenly arranged on the disk body circumferencial direction of the second filter disc (11)
1), the inner circle circumferential direction of application cylinder supporting leg (1-1) is provided with convex along (1-2), is provided with and second on (1-2) in convex
The recess of positioning protrusion (11-1) cooperation of filter disc (11), the second filter disc (11) are overlapped on convex on (1-2).
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 8. according to claim 1, it is characterised in that are planted
Through-hole aperture on the barrel of object growth room (1) and the room side wall (2-2) of hyphal development room (2) is 0.5~1cm.
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 9. according to claim 1, it is characterised in that are planted
The aperture of micropore on object growth room (1) bottom wall and the micropore on hyphal development room (2) bottom wall is 1~2mm.
Split type polycyclic type mycorhiza locellus culture and processing unit are revolved 10. according to claim 1, it is characterised in that are planted
There are fluid course, the liquid between the cylinder bottom of the bottom wall and experiment control cylinder (3) of object growth room (1) and hyphal development room (2)
The height h of circulation road is 3~5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811052958.XA CN109251843B (en) | 2018-09-10 | 2018-09-10 | Split type rotatable multi-ring type mycorrhiza separate room cultivation and treatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN109251843B (en) | 2021-05-07 |
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