CN109221026A - A kind of efficient device and method for collecting Bradysia odoriphaga ovum - Google Patents
A kind of efficient device and method for collecting Bradysia odoriphaga ovum Download PDFInfo
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- CN109221026A CN109221026A CN201811380579.3A CN201811380579A CN109221026A CN 109221026 A CN109221026 A CN 109221026A CN 201811380579 A CN201811380579 A CN 201811380579A CN 109221026 A CN109221026 A CN 109221026A
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- collection box
- ovum
- bradysia odoriphaga
- culture dish
- leek
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- 241000613693 Bradysia odoriphaga Species 0.000 title claims abstract description 75
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 69
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 69
- 210000004681 ovum Anatomy 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000017448 oviposition Effects 0.000 claims abstract description 31
- 235000013601 eggs Nutrition 0.000 claims abstract description 26
- 240000006108 Allium ampeloprasum Species 0.000 claims description 53
- 235000005254 Allium ampeloprasum Nutrition 0.000 claims description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 229920001817 Agar Polymers 0.000 claims description 11
- 239000008272 agar Substances 0.000 claims description 11
- 239000003086 colorant Substances 0.000 claims description 11
- 238000002474 experimental method Methods 0.000 claims description 9
- 230000029305 taxis Effects 0.000 claims description 7
- 238000012360 testing method Methods 0.000 claims description 6
- 235000018645 Allium odorum Nutrition 0.000 claims description 4
- 240000008654 Allium ramosum Species 0.000 claims description 4
- 241001494113 Bradysia Species 0.000 claims description 2
- 241001157791 Mycetophilus sp. HMR-1993 Species 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 5
- 230000000694 effects Effects 0.000 description 7
- 238000013461 design Methods 0.000 description 4
- 238000004088 simulation Methods 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000003020 moisturizing effect Effects 0.000 description 2
- 241000892865 Heros Species 0.000 description 1
- 238000001792 White test Methods 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of devices for efficiently collecting Bradysia odoriphaga ovum, including culture dish, the collection box being placed in culture dish, the collection box is the structure that bottom is closed, an aperture is reserved at top, tray interior is hollow, reserves certain space between the outer wall at the top of collection box and the inner wall of culture ware lid；Dark-coloured paster is all being posted around the collection box inner and outer wall.Also disclose a kind of method for efficiently collecting Bradysia odoriphaga ovum.The present invention has apparent efficiently collection effectiveness, it can not only be trapped using the method and be laid eggs in Bradysia odoriphaga adult fixed-point set, egg laying amount can also be obviously increased, the collection efficiency of Bradysia odoriphaga ovum in laboratory is significantly improved, provides new method efficiently to collect Bradysia odoriphaga ovum.Box device is collected using self-control, provided device is not only simple in structure, and it is easy to operate, and convenient for the efficient process for completing to artificially collect ovum, improve feeding efficiency.
Description
Technical field
The invention belongs to entomology, Plant Protection technical field, and in particular to a kind of efficiently to collect Bradysia odoriphaga
The device and method of oviposition.
Background technique
The larva of Bradysia odoriphaga is commonly called as " bradysia odoriphaga ", endangers leek, can cause underproduction 40%-80%, is leek industry
Crushing pest.Adult not feeding has stronger taxis to leek, in soil seam, the plant base of leek field earth's surface after emergence
The dispersion oviposition of the sheltered situations such as portion.Generally adult is monitored and is prevented and treated using yellow haftplatte in production, but yellow whether
For the most thermophilic color of the adult, relevant report is but had no.Field is used cooperatively using nematode and black haftplatte to Bradysia odoriphaga
Control efficiency it is preferable.Adult fixed point is trapped indoors, concentrates and receives whether ovum is feasible, and most critical is exactly oviposition according to adult
Habit, it is suitable for its spawning place that simulation, which is built,.Since adult can often originate in ovum in the soil of leek bulb base portion, have obvious
The dark habit that becomes find a kind of effective method and efficiently collect ovum grain therefore, it is necessary to the dark habit that becomes using adult.
Existing bradysia odoriphaga artificial feeding device is mainly based on culture dish, during the feeding process, with the filter paper moisturizing for adding water,
Often because addition water control is bad, overdrying or overly moist bradysia odoriphaga mortality is easily led to；In addition, being cultivated when adult mate and oviposit
Zone of action in ware is not fixed, and is often resulted in ovum grain and is collected difficulty, and the operation for collecting ovum grain is time-consuming and laborious, can also be because excessively artificial
Interference leads to ovum grain hatching rate degradation.In addition, the freshness of leek it could also be possible that influence collect one of ovum amount because
One of element.Collecting the technical research of Bradysia odoriphaga ovum about experiment indoor highly effective at present is almost blank.
Summary of the invention
The present invention provides a kind of efficiently collection Bradysia odoriphaga for high investment, the deficiencies in the prior art of low output
The device and method of ovum, the present invention have it is apparent collect effectiveness, can not only be trapped using the method Bradysia odoriphaga at
It lays eggs in worm fixed-point set, can also obviously increase egg laying amount, significantly improve the collection efficiency of Bradysia odoriphaga ovum in laboratory,
New method is provided efficiently to collect Bradysia odoriphaga ovum.The present invention collects box device using self-control, and provided device is not
Only structure is simple, easy to operate, and the process of ovum is artificially collected convenient for efficiently completion, improves feeding efficiency.
To achieve the above object, the present invention adopts the following technical scheme: an aspect of of the present present invention provides a kind of efficiently collection
The device of Bradysia odoriphaga ovum, including culture dish, the collection box being placed in culture dish, the collection box be bottom closing,
Reserve the hollow structure of an aperture, tray interior in top；One is reserved between outer wall at the top of collection box and the inner wall of culture ware lid
Fixed space；Dark-coloured paster is being posted around the collection box inner and outer wall.The bottom of collection box is close-coupled at culture dish
Bottom adds on agar-filter paper of water, and the hollow environment of relative closure inside collection box is practised according to Bradysia odoriphaga Adult worms producting eggs
Property, it is suitable for its spawning place that simulation, which is built,.
Preferably, using water agar-filter paper shop fixtures is added, water and agar ratio are 50:1 for bottom in the culture dish,
Filter paper is attached to plus the top of water agar, and the bottom of the collection box is close-coupled above filter paper.In the culture dish, fall first
Enter boiled agar in proportion (water and agar ratio be 50:1), filter paper is laid with above agar, filter paper has water imbibition, no
It needs to add water；Collection box is placed in ware above filter paper；For guaranteeing the suitable humidity of bradysia odoriphaga raising.
Preferably, the collection box is designed as square, and side length is 2~3cm.
Preferably, the side length of the collection box is 2.5cm.
Preferably, the color that the collection box inner and outer wall pastes dark-coloured paster is black or green.
Preferably, the diameter of the culture dish selects 15cm.
Another aspect of the present invention provides a kind of method for collecting Bradysia odoriphaga ovum using above-mentioned device, including following
Step:
1. same volume culture dish, same volume color collection box, same leek are placed in collection box: being distributed on leek different
Bradysia odoriphaga adult sex ration, determine optimal sex ration；
2. same volume culture dish, the collection box of same volume different colours, same leek: in conjunction with optimal sex ration,
In the paster of the inside and outside wall patch different colours of collection box；Observation Adult worms producting eggs have taxis to which kind of color；
3. same volume culture dish, the collection box of different spaces size, same leek: in conjunction with optimal sex ration, paster
Color, is set as different volumes collection box, and the ovum that observation bradysia odoriphaga is collected under which kind of space size is most；
4. the culture dish of different spaces size, same leek: in conjunction with optimal sex ration, paster color, collection box volume,
The case where being set as different volumes culture dish, checking oviposition；
5. leek difference freshness: in conjunction with optimal sex ration, paster color, collection box volume, culture dish volume, putting
Enter different freshness leek；The influence that observation leek difference freshness lays eggs to bradysia odoriphaga.
Preferably, step 1., 4., 5. in it is each experiment be at least repeated 5 times.
Preferably, 2., 3. each test at least is repeated 10 times step.
Preferably, step is 2., black, yellow, white, green different colours paster is pasted in the inside and outside wall of collection box.
Beneficial effects of the present invention:
1) present invention has apparent efficiently collection effectiveness, can not only trap Bradysia odoriphaga adult using the method
It lays eggs in fixed-point set, can also obviously increase egg laying amount, significantly improve the collection efficiency of Bradysia odoriphaga ovum in laboratory, be
It efficiently collects Bradysia odoriphaga ovum and provides new method.
2) present invention collects box device using self-control, and provided device is not only simple in structure, easy to operate, and convenient for height
Effect is completed to artificially collect the process of ovum, improves feeding efficiency.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is the apparatus structure schematic diagram for collecting Bradysia odoriphaga ovum；
Fig. 2 is influence of the different sex rations to bradysia odoriphaga egg laying amount；
Fig. 3 is influence of the inside and outside wall paster color of different collection boxes to bradysia odoriphaga egg laying amount；
Fig. 4 is influence of the leek difference freshness to bradysia odoriphaga egg laying amount.
In figure, 1, culture dish, 2, collection box, 3, dark-coloured paster, 4, hole, 5, leek, 6 plus water agar-filter paper.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
As background technology part is introduced, whether yellow is the most thermophilic color of the adult, but has no relevant report.Field
Using nematode with black haftplatte with the use of preferable to the control efficiency of Bradysia odoriphaga.Adult is trapped indoors carries out receipts ovum
Whether feasible, most critical is exactly spawning habit according to adult, and it is suitable for its spawning place that simulation, which is built,.Since adult can often incite somebody to action
Ovum originates in the soil of leek bulb base portion, has the dark habit that significantly becomes.Existing bradysia odoriphaga breeding apparatus is mainly with culture dish
Based on, during the feeding process, cause overdrying or overly moist often because the water control of addition is bad with the filter paper shop fixtures moisturizing for adding water,
Lead to bradysia odoriphaga mortality.
In addition, the zone of action in culture dish is not fixed when adult mate and oviposit, often results in ovum grain and collect difficult, receipts
The operation for collecting ovum grain is time-consuming and laborious, and ovum grain hatching rate degradation can be also caused because of excessive human interference.In addition, leek is fresh
Degree is it could also be possible that influence an one of factor for collection ovum amount.Bradysia odoriphaga is collected about experiment indoor highly effective at present
The technical research of ovum is almost blank.The invention to the poor selectivity opposite sex of color and is produced using Bradysia odoriphaga
Ovum space size compares research to the collecting effect of adult, it is determined that under which kind of sex ration, which kind of volume, collects dress
It is best to set most interior quantity for being collected into ovum, efficiency highest, effect.
The device for proposing following one kind and efficiently collecting Bradysia odoriphaga ovum of the invention, including culture dish 1,
The collection box 2 being placed in culture dish 1, the collection box 2 are that bottom is closed, an aperture 4 is reserved at top, tray interior is hollow
Structure；Certain space is reserved between the outer wall at 2 top of collection box and the inner wall for cultivating ware lid；In the collection box 2
Wall and outer wall post dark-coloured paster 3.
Leek 5 is placed in collection box 2, the bottom of collection box 2 is close-coupled at bottom in culture dish 1 plus water agar-
6 top of filter paper, the hollow environment of 2 inside relative closure of collection box, according to Bradysia odoriphaga Adult worms producting eggs habit, simulation is built
It is suitable for its spawning place.；Dark-coloured paster 3 is being posted around 2 inner and outer wall of collection box, is being because Adult worms producting eggs are to half-light
There is taxis with color, most ovum can be obtained.The bottom of collection box is closely connect with culture dish, is as much as possible for fragrant-flowered garlic
Maggot provides a relatively hidden, fixed egg-laying environment；The effect of aperture 4 --- it is that bradysia odoriphaga adult is collected from the entrance of culture dish 1
Then entrance in box 2 is laid eggs near the leek bulb in collection box 2.Leek bulb is placed in collection box 2, is lured
Collect the bradysia odoriphaga adult in culture dish 1, by aperture 4, into collection box 2 in concentrate oviposition, greatly improve spawning rate, while side
Just centralized collection worm's ovum.
In the present embodiment, with adding water agar-filter paper 6 to carry out shop fixtures, water and agar ratio are for bottom in the culture dish 1
50:1, filter paper is attached to plus the top of water agar, and the bottom of collection box 2 is close-coupled above filter paper.For providing bradysia odoriphaga raising
Suitable humidity.
In culture dish, boiled agar in proportion is poured into first, filter paper is laid with above agar, and filter paper has water suction
Property, it does not need to add water；Collection box is placed in ware above filter paper, needs to place leek bulb in the collection box, adult can be in fragrant-flowered garlic
Dish bulb is nearby laid eggs, and can arbitrarily be laid eggs in ware to avoid adult in this way, be greatlyd improve spawning rate, convenient for collecting ovum.
The collection box 2 is designed as square, and side length is 2~3cm；Preferably, the side length of the collection box 2 is
2.5cm.The color of dark-coloured paster 3 is black or green in the collection box 2.The diameter of the culture dish 1 selects 15cm.
Another aspect of the present invention provides a kind of method for efficiently collecting Bradysia odoriphaga ovum using above-mentioned device, including
Following steps:
1. same volume culture dish, same volume paster color collection box, same leek are placed in collection box: being distributed on leek
The sex ration of different Bradysia odoriphaga adults, determines optimal female and male ratio；Each experiment is at least repeated 5 times；
2. same volume culture dish, the collection box of same volume difference paster color, same leek: in conjunction with optimal male and female
Than pasting black, yellow, white, green different colours paster in the inside and outside wall of collection box；Observation Adult worms producting eggs have taxis to which kind of color；Often
A experiment is at least repeated 10 times.
3. same volume culture dish, the collection box of different spaces size, same leek: in conjunction with optimal sex ration, paster
Color, is set as different volumes collection box, and the ovum that observation bradysia odoriphaga is collected under which kind of space size is most；Each experiment at least repeats
10 times.
4. the culture dish of different spaces size, same leek: in conjunction with optimal sex ration, paster color, collection box volume,
The case where being set as different volumes culture dish, checking oviposition；Each experiment is at least repeated 5 times；
5. leek difference freshness: in conjunction with optimal sex ration, paster color, collection box volume, culture dish volume, putting
Enter the leek of different freshness；The influence that observation leek difference freshness lays eggs to bradysia odoriphaga；Each experiment at least repeats 5
It is secondary.
Method of the invention designs Bradysia odoriphaga adult female-male proportion first, determines optimal sex ration, improves fragrant-flowered garlic
Maggot oviposition quantity；Then the taxis of half-light and color is used on the inside and outside wall of collection box according to Bradysia odoriphaga adult
The paster of black, white, yellow, green four kinds of colors enhances gathering effect；Test is to 4 kinds of color preference othernesses and its space size
Collecting effect and leek freshness to Bradysia odoriphaga adult are studied.Room temperature, no striation are placed on after collecting ovum
Under part.
Test determines optimal sex ration by design Bradysia odoriphaga adult difference female-male proportion；Become according to adult
Dark property explores hobby of the adult to color in laboratory；It determines the influence under different spaces to Adult worms producting eggs amount, is next
The space utilization rate of step provides theoretical foundation；Influence of the leek freshness to Adult worms producting eggs is tested, convenient for improving leek Bradysia
Fungus gnat oviposition quantity.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Test material used in the embodiment of the present invention is the test material of this field routine, can pass through commercial channel
It is commercially available.
Embodiment 1:
(1) same volume culture dish 1, same volume color collection box 2, same leek 5 are placed in collection box 2:
In the culture dish 1 of diameter 9cm, female and male ratio is respectively 1:1,1:3 and 3:1,3:2 and 2:3, is determined best female
Male ratio, obtains most ovum；Each it is repeated 5 times.
If Fig. 2 designs different sex rations in the culture dish 1 of same volume size, as a result, it has been found that in 15 female 5 heros
Under ratio, Bradysia odoriphaga egg laying amount highest is 861 ovum, is secondly, under 10 female 5 male ratios, egg laying amount 690,
5 female 5 is male, minimum 5 female 15 male egg laying amounts are respectively 383 and 398 ovum；According to the quantity of harvest ovum, best male and female proportion is determined
It is male for 15 female 5.
(2) same volume culture dish 1, the collection box 2 of same volume different colours, same leek 5:
In the culture dish of diameter 9cm, in conjunction with optimal sex ration above, optimal different colours are set as in collection box
It is set separately are as follows: black, yellow, white, green；Observation Adult worms producting eggs have taxis to which kind of color, obtain most ovum；Each repetition 10
It is secondary.
Such as Fig. 3, in the culture dish of same volume size, 4 kinds of different colours of experimental design lay eggs to Bradysia odoriphaga
Influence, be respectively as follows: black, white, green and yellow hobby of the observation adult to color, the results showed that black and other color differences and
Significantly, the ovum that black is collected into is most, is 392 ovum, is secondly green 303 ovum, it is 71 that white ovum amount of receiving is worst.It receives
Ovum effect is from more to less successively are as follows: black > green > yellow > white tests and determines that black collection box collects the quantity of ovum most
It is good.
(3) same volume culture dish 1, the collection box 2 of different spaces size, same leek: in diameter 9cm culture dish, if
The influence that the black collection box of meter different spaces size lays eggs to bradysia odoriphaga.
By above it is demonstrated experimentally that adult is in the collection box of black, the quantity of ovum is collected, according to different spaces size
The influence laid eggs to Bradysia odoriphaga of black collection box, be set to large, medium and small three volumes, respectively side length 3cm,
2.5cm and 2cm.The ovum that observation Bradysia odoriphaga is collected under which kind of space size is most, is each repeated 10 times.
Schemed by following it is found that being apparently higher than side length 3cm and 2cm, difference in the ovum that the collection box of side length 2.5cm is collected into
Significantly.Under the black collection box of side length 2.5cm, collecting ovum amount is 356, compared with side length 3cm and 2cm, has increased separately 130
With 209 ovum.
(4) culture dish 1 of different spaces size, same leek: the black collection box 2 of side length 2.5cm designs different spaces
The influence that the culture dish 1 of size lays eggs to bradysia odoriphaga, influence of the different spaces size to Adult worms producting eggs are set to large, medium and small three
Volume, in diameter 5.5cm, 9cm, 15cm culture dish, the case where checking oviposition.Each it is repeated 5 times.
As known to the following table 1, the ovum that Bradysia odoriphaga is collected in diameter 15cm culture dish 1 is most, is 974.With it is straight
Diameter 5.5cm, 9cm are compared, difference and its significant.As space becomes smaller, egg laying amount is gradually decreased.The experimental results showed that with space
Increase, Bradysia odoriphaga egg laying amount increases.Determine the egg laying amount highest in 15m culture dish.
The influence to Bradysia odoriphaga egg laying amount of table 1, different volumes collection box and culture dish
(5) influence that the different freshness of leek 5 lay eggs to Bradysia odoriphaga
In the culture dish 1 of diameter 15cm, the collection box 2 of side length 2.5cm is respectively put into 5 freshness of leek are as follows: it is fresh, 1 day,
2 days and 3 days.The influence that the different freshness of observation leek 5 lay eggs to Bradysia odoriphaga.
As shown in Figure 4, having a direct impact with leek freshness to Bradysia odoriphaga egg laying amount, leek is fresher,
Egg laying amount is more.Under fresh state, egg laying amount be 924, with storage 1 day, 2 days, 3 days egg laying amount difference it is particularly significant,
It is secondary leek store 1 day after, egg laying amount 834；After leek is stored 2 days, egg laying amount 684；After leek is stored 3 days, egg laying amount
It is 434.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of device for efficiently collecting Bradysia odoriphaga ovum, special including culture dish, the collection box being placed in culture dish
Sign is: the collection box is the structure that bottom is closed, an aperture is reserved at top, tray interior is hollow；It is outer at the top of collection box
Certain space is reserved between wall and the inner wall for cultivating ware lid；Dark-coloured paster is being posted around the collection box inner and outer wall.
2. the efficient device for collecting Bradysia odoriphaga ovum according to claim 1, it is characterised in that: in the culture dish
Interior bottom is using water agar-filter paper shop fixtures is added, and water and agar ratio are 50:1, and filter paper is attached to plus the top of water agar, the receipts
The bottom of collection box is close-coupled above filter paper.
3. the efficient device for collecting Bradysia odoriphaga ovum according to claim 1, it is characterised in that: the collection box is set
It is calculated as square, and side length is 2~3cm.
4. the efficient device for collecting Bradysia odoriphaga ovum according to claim 3, it is characterised in that: the collection box
Side length is 2.5cm.
5. the efficient device for collecting Bradysia odoriphaga ovum according to claim 1, it is characterised in that: in the collection box
The color that wall and outer wall paste dark-coloured paster is black or green.
6. the efficient device for collecting Bradysia odoriphaga ovum according to claim 1, it is characterised in that: the culture dish
Diameter selects 15cm.
7. a kind of method for collecting Bradysia odoriphaga ovum using device described in claim 1, it is characterised in that: including following
Step:
1. same volume culture dish, same volume color collection box, same leek are placed in collection box: distributing different fragrant-flowered garlic on leek
Dish Bradysia fungus gnat sex ration, determines optimal sex ration；
2. same volume culture dish, the collection box of same volume different colours, same leek: in conjunction with optimal Bradysia odoriphaga
Sex ration pastes different colours paster in the inside and outside wall of collection box；Observation Adult worms producting eggs have taxis to which kind of color；
3. same volume culture dish, the collection box of different spaces size, same leek: in conjunction with optimal sex ration, paster color,
It is set as the collection box of different volumes, observation bradysia odoriphaga is laid eggs at most under the space size of which kind of collection box；
4. same leek: the culture dish of different spaces size in conjunction with optimal sex ration, paster color, collection box volume, is set as
The case where culture dish of different volumes, observation oviposition；
5. leek difference freshness: in conjunction with optimal sex ration, paster color, collection box volume, culture dish volume, being put into not
With freshness leek；The influence that observation leek difference freshness lays eggs to bradysia odoriphaga.
8. the method according to claim 7 for collecting Bradysia odoriphaga ovum, it is characterised in that: step 1., 4., 5. in it is every
A experiment is at least repeated 5 times.
9. the method according to claim 7 for collecting Bradysia odoriphaga ovum, it is characterised in that: step 2., 3. each reality
It tests and is at least repeated 10 times.
10. it is according to claim 7 collect Bradysia odoriphaga ovum method, it is characterised in that: step 2., in collection box
Inside and outside wall pastes the paster of black, yellow, white, green different colours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811380579.3A CN109221026B (en) | 2018-11-20 | 2018-11-20 | Device and method for efficiently collecting slow-eye mushroom mosquito eggs of Chinese chives |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811380579.3A CN109221026B (en) | 2018-11-20 | 2018-11-20 | Device and method for efficiently collecting slow-eye mushroom mosquito eggs of Chinese chives |
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| Publication Number | Publication Date |
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| CN109221026A true CN109221026A (en) | 2019-01-18 |
| CN109221026B CN109221026B (en) | 2023-09-22 |
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| CN201811380579.3A Active CN109221026B (en) | 2018-11-20 | 2018-11-20 | Device and method for efficiently collecting slow-eye mushroom mosquito eggs of Chinese chives |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110122424A (en) * | 2019-05-15 | 2019-08-16 | 山东省农业科学院植物保护研究所 | A kind of device trapping coccinella septempunctata oviposition |
| CN110122426A (en) * | 2019-05-15 | 2019-08-16 | 山东省农业科学院植物保护研究所 | A method of trapping coccinella septempunctata oviposition |
| CN110122424B (en) * | 2019-05-15 | 2021-10-26 | 山东省农业科学院植物保护研究所 | Device for inducing ladybug to lay eggs |
| CN110122426B (en) * | 2019-05-15 | 2021-11-26 | 山东省农业科学院植物保护研究所 | Method for trapping coccinella septempunctata to lay eggs |
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