JPH1042766A - Artificial collection of egg of lepidopterous insect and artificial egg collection device therefor - Google Patents

Abstract

PROBLEM TO BE SOLVED: To collect eggs of lepidopterous insect in high yield continuously without using raw leaves of a parasitic plant of lepidopterous insect by using an egg collecting solution obtained from a specific plant in which the lepidopterous insect lives and collecting the eggs of lepidopterous insect indoors and outdoors. SOLUTION: A lepidopterous insect is made to lay eggs on at least the back side of a laminar main body holding an egg collecting solution having 5-50mg/mL raw leaf concentration obtained from an extracted stock solution of raw leaves of a parasitic plant of the lepidopterous insect in a liquid retaining member attached to the surface side of the laminar main body.

Description

【発明の詳細な説明】DETAILED DESCRIPTION OF THE INVENTION

【０００１】[0001]

【発明の属する技術分野】本発明は鱗翅目昆虫の人工採
卵法およびその人工採卵装置に関し、更に詳しくは、鱗
翅目昆虫が摂食や産卵行動をとる際に寄生する特定の植
物、所謂寄主植物の生葉抽出原液から得られた採卵液を
用いて屋内外で鱗翅目昆虫の採卵を行なう人工採卵法、
およびその人工採卵法に用いる人工採卵装置に関する。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for artificially collecting lepidopteran insects and an artificial egg collecting apparatus therefor, and more particularly, to a specific plant, a so-called host plant, which is parasitic when a lepidopteran insect takes a feeding or spawning behavior. An artificial egg collection method for collecting lepidopteran insects indoors and outdoors using an egg collection solution obtained from a raw leaf extract stock solution,
And an artificial egg collecting apparatus used for the artificial egg collecting method.

【０００２】[0002]

【従来の技術】蝶・蛾等の鱗翅目昆虫は重大な農業害虫
である。年間の害虫の発生数を調べると、越冬世代、第
一世代、第二世代と世代を繰り返す毎に急激に発生数は
増加する。これは雌一頭が一回当たり数百個も産卵する
ためであり、親世代では少数であっても子世代では爆発
的な害虫発生が起こることがしばしばある。従って、鱗
翅目昆虫の交尾・産卵行動を妨害することや、成虫を捕
獲することは有効な害虫駆除法である。2. Description of the Related Art Lepidopteran insects such as butterflies and moths are important agricultural pests. Examining the number of pests that occur annually, the numbers increase rapidly with each repetition of the overwintering generation, the first generation, and the second generation. This is because one female lays hundreds of eggs at a time, and even a small number in the parent generation often causes explosive pest outbreaks in the child generation. Therefore, interfering with the mating and spawning behaviors of lepidopteran insects and capturing adults are effective pest control methods.

【０００３】これまで、蝶・蛾等の鱗翅目昆虫採卵に
は、鱗翅目昆虫の寄主植物を野外から採集したり、或い
は寄主植物を温室などで大量に栽培し、鉢植え寄主植物
を採卵室内に設置し、寄主植物の葉の裏面に産卵させ、
これを採卵する方法が一般的であった。Hitherto, for collecting eggs of lepidopteran insects such as butterflies and moths, host plants of lepidopteran insects have been collected from the field, or host plants have been cultivated in large quantities in greenhouses and potted host plants have been placed in the egg collecting chamber. Install and lay eggs on the back of the host plant's leaves,
The method of collecting this was common.

【０００４】そこで鱗翅目昆虫の寄主植物の生葉を用い
て採卵を行なう場合に用いる採卵室の１例について述べ
る。図９に示すように前面に開閉自在の扉付き木製の箱
(73×85×115cm)から成る採卵室ａの内部を艶消しの黒
い紙で覆い、上部に15ワットの蛍光灯ｂを取り付け、オ
ートタイマーｃで16L-8Dの長日条件に設定した。温度は
25〜27℃、湿度は65〜80％ＲＨを目標とした。乾燥条件
下では産卵数が極度に減少するため、採卵室ａの底面側
の孔あき仕切板ｄの下方にに水を入れた容器ｅを設置
し、更に冬期間はタイムスイッチを接続した加湿器を用
いて間欠的に補湿して目標湿度を維持するようにした。
成虫の吸蜜源としてプラスチック製容器ｆ(例えば200m
l)内に20％蔗糖液を入れ、6〜7個の孔のあいた蓋をし、
その中央に造花ｇを一輪さした。[0004] An example of an egg collection room used for egg collection using the leaves of a host plant of a lepidopteran insect will now be described. A wooden box with a door that can be opened and closed on the front as shown in FIG.
(73 × 85 × 115 cm) The inside of the egg collection room a was covered with matte black paper, a 15 watt fluorescent lamp b was attached on the upper part, and the auto timer c was set to the long day condition of 16L-8D. The temperature is
The target was 25-27 ° C. and the humidity was 65-80% RH. Under dry conditions, the number of eggs laid extremely decreases. Therefore, a container e filled with water is installed below the perforated partition plate d on the bottom side of the egg collection room a, and a humidifier connected with a time switch during the winter period. And the target humidity was maintained by intermittent humidification.
A plastic container f (for example, 200 m
l) Put 20% sucrose solution into it, cover with 6 ~ 7 holes,
One artificial flower g was wrapped in the center.

【０００５】また、完全な暗条件では産卵しないことか
ら、蜜源のプラスチック製容器ｆ近傍に照明器ｈを配置
して低照度(3〜5lux)で常時照らすようにした。産卵場
所として採卵室ａの中央に鉢植えの鱗翅目昆虫の寄主植
物ｉ、例えばサツマイモ苗を配置すると共に、寄主植物
ｉの前方に羽化前のサナギを入れる籠ｊを配置した。[0005] In addition, since no eggs are laid in a completely dark condition, an illuminator h is arranged near the plastic container f of the honey source so as to constantly illuminate at low illuminance (3 to 5 lux). A host plant i of potted lepidopteran insects, for example, a sweet potato seedling, was placed in the center of the egg collection room a as an egg-laying place, and a basket j for putting pupae before emergence was placed in front of the host plant i.

【０００６】また、採卵を行なう鱗翅目昆虫、例えばエ
ビガラスズメの蛹は幼虫期の飼育条件により休眠・非休
眠化するので、採卵に供する成虫を得るためには、幼虫
期の日長条件を制御して非休眠蛹を誘導するか、もしく
は休眠蛹を低温で保護して休眠を覚醒する必要がある。
通常、幼虫期を27±1℃、16L-8Dの高温・日長条件で飼
育することによって非休眠蛹を誘導するようにしてい
る。採卵は一日一回明期に行い、その際、死亡あるいは
衰弱した蛾は取り除き、採卵室（飼育箱）内を出来るだ
け清浄に保つように努める。In addition, lepidopteran insects that collect eggs, such as the pupae of shrimp murmurs, are dormant and non-dormant depending on the larval rearing conditions. It is necessary to induce non-dormant pupae to wake up, or to wake dormancy by protecting dormant pupae at low temperatures.
Normally, non-dormant pupae are induced by rearing the larvae under the conditions of 27 ± 1 ° C and 16L-8D at high temperature and photoperiod. Eggs are collected once a day during the light period, in which case dead or weak moths are removed and efforts are made to keep the inside of the egg collection room (breeding box) as clean as possible.

【０００７】尚、前記採卵室の構造およびエビガラスズ
メの採卵については「エビガラスズメの通年飼育体系」
蚕糸・昆虫農業技術研究所 研究報告 第10号（第38頁な
いし第39頁）平成６年３月より引用した。[0007] The structure of the egg collection room and the egg collection of shrimp grouse are described in "System for rearing shrimp grouse all year round".
Research Institute for Silk and Insect Agriculture, Research Report No. 10 (pages 38 to 39), cited in March 1994.

【０００８】そこで、鱗翅目昆虫を駆除するにあたっ
て、蝶・蛾等の鱗翅目昆虫の寄主特異性、産卵刺激物
質、産卵行動、鱗翅目昆虫の採卵法について説明する。[0008] Therefore, in controlling lepidopteran insects, host specificity, spawning stimulants, spawning behavior, and a method of collecting lepidopteran insects will be described.

【０００９】 寄主特異性 一般に、蝶・蛾等の鱗翅目昆虫は特定の植物である所謂
寄主植物に対してのみ摂食や産卵行動をとる。例えば、
蛾の一種であるエビガラスズメの幼虫はサツマイモの葉
だけを食べ、成虫はサツマイモの葉だけに産卵するとい
う習性を有している。鱗翅目昆虫は昆虫自体の寄主植物
に含まれている微量な二次代謝産物（化学物質）を感知
する能力があり、その刺激を受けて初めて摂食や産卵行
動が誘発されると考えられている。Host Specificity In general, lepidopteran insects such as butterflies and moths feed and lay eggs only on specific plants, so-called host plants. For example,
The larva of the shrimp, a kind of moth, eats only sweet potato leaves, and the adult has the habit of laying eggs only on sweet potato leaves. Lepidopteran insects have the ability to sense minute amounts of secondary metabolites (chemical substances) contained in the insect's own host plants, and it is thought that feeding and spawning behavior are induced only by the stimulation. I have.

【００１０】 産卵刺激物質 現在のところ、交尾した雌に産卵行動を誘発する物質、
例えばアゲハチョウ科の蝶では数種の産卵刺激物質（フ
ラボノイド、アルカノイド類）が同定されているが、そ
の他の蝶・蛾等の鱗翅目昆虫では全く分かっていない。Oviposition stimulants At present, substances that induce spawning behavior in mated females,
For example, several spawning stimulants (flavonoids, arkanoids) have been identified in the butterfly of the Papilionidae, but are not known at all for lepidopteran insects such as butterflies and moths.

【００１１】 産卵行動 ジャコウアゲハでは葉の表面側を前脚でたたき（ドラミ
ング行動という）、前脚先端部にある感覚毛で産卵刺激
物質を感知して、葉の裏面側に産卵する。エビガラスズ
メではドラミング行動はとらないが、静止飛行（ホバリ
ング行動という）しながら葉の表面側に前脚を接触さ
せ、やはり葉の裏面側に産卵する。Spawning behavior In Musk Swallowtail, the front side of a leaf is hit with a front leg (referred to as a drumming behavior), and the sperm at the tip of the front leg senses an egg-laying stimulating substance to lay eggs on the back side of the leaf. Shrimp glass sparrows do not take a drumming action, but contact their front legs with the front side of the leaf while flying still (hovering action), and also lay eggs on the back side of the leaf.

【００１２】 採卵法 現在のところ蝶・蛾等鱗翅目昆虫の採卵法としては、野
外から採集した寄主植物、或いは温室などで大量に栽培
した寄主植物の生葉に産卵させて、生葉から卵を１粒ず
つ取り外して採卵する方法が一般的である。At present, as a method for collecting lepidopteran insects such as butterflies and moths, eggs are collected on a host plant collected from the field or on a fresh leaf of a host plant cultivated in a large amount in a greenhouse or the like. It is common to remove eggs one by one and collect eggs.

【００１３】蝶類では、ジャコウアゲハ等の一部の種
で、寄主植物の生葉のメタノール抽出液に浸したフィル
ターペーパーに雌が産卵することが僅かに確認されてい
る程度であり、継続的に使用することが出来る採卵法は
存在していない現状である。[0013] In some butterfly species, such as the muskwing swallowtail, it has been confirmed that females lay eggs on filter paper soaked in a methanol extract of the leaves of the host plant. There is no egg collection method that can do this.

【００１４】蛾類では、イラクサギンウワバ等の寄主植
物を初から必要としない（つまり採卵装置も必要としな
い）性質を持った一部の種を除くと、エビガラスズメの
採卵法にみられるように寄主植物の生葉は必要である。In moths, except for some species having properties that do not require host plants such as nettle worts from the beginning (that is, they do not require an egg collecting device), as seen in the egg collecting method of shrimp murmurs. Fresh leaves of the host plant are required.

【００１５】また、蛾類についても蝶類と同様に、寄主
植物の生葉のメタノール抽出液をフィルターペーパー等
に染み込ませたものを用いて採卵を行なう方法が試みら
れたが、実用にはほど遠いものであった。As for moths, similar to butterflies, a method of collecting eggs using a methanol extract of fresh leaves of a host plant impregnated with filter paper or the like has been attempted, but is far from practical. Met.

【００１６】人工採卵法の試みとしては、唯一タバコス
ズメガの例がある。これは、円形状のウレタンフォーム
にタバコ葉の80％エタノール抽出液を浸し、これをポリ
エチレンフィルターまたはコルク板に載置し、ポリエチ
レンフィルターまたはコルク板に産卵させて採卵する方
法がある。[0016] The only attempt of artificial egg collection method is tobacco tobacco. In this method, an 80% ethanol extract of tobacco leaves is immersed in a circular urethane foam, placed on a polyethylene filter or a cork plate, and laid on a polyethylene filter or a cork plate to collect eggs.

【００１７】[0017]

【発明が解決しようとする課題】しかしながら、これま
で鱗翅目昆虫の寄主植物の誘引、産卵刺激物質はほとん
ど同定されておらず、特に蛾類は夜行性であることか
ら、これら蛾類の寄主植物の誘引、産卵刺激物質の検索
が困難であった。However, there has been little identification of host and plant spawning stimulants of lepidopteran insects, and since moths are nocturnal, host plants of these moths have not been identified so far. And it was difficult to search for spawning stimulants.

【００１８】前述のように、蝶・蛾等の鱗翅目昆虫の実
用的な人工採卵法はこれまでに開発されておらず、多く
の場合、寄主植物の生葉、または鉢植え寄主植物を用
い、これに鱗翅目昆虫に産卵させて、これを採卵する方
法であった。As described above, a practical artificial egg collection method for lepidopteran insects such as butterflies and moths has not been developed so far, and in many cases, fresh leaves of a host plant or potted host plants are used. And laid eggs by lepidopteran insects and collected eggs.

【００１９】従って、寄主植物を野外から採集してきた
り、または温室等で大量に栽培するする必要があった。
本発明者らの種々の実験結果では例えばエビガラスズメ
の場合、一年間に鉢植えのサツマイモを100個必要とし
た。更に、産み付けられた卵は寄主植物の葉から一粒ず
つ取り外して採卵する必要があった。Therefore, it was necessary to collect the host plants from the field or to cultivate them in large quantities in a greenhouse or the like.
According to the various experimental results of the present inventors, for example, in the case of shrimp glass sparrows, 100 potatoes were required for one year. Furthermore, the laid eggs had to be removed from the host plant one by one and collected.

【００２０】また、前述のように蝶類のジャコウアゲハ
の一部で寄主植物の生葉のメタノール抽出液に浸したフ
ィルターペーパーに雌が産卵することが確認されていて
も、これを継続的に産卵させ、採卵を行なう手段は今だ
に開発されていない。As described above, even if it has been confirmed that females lay eggs on filter paper dipped in a methanol extract of the leaves of the host plant, it is possible to continuously lay eggs. However, no means of collecting eggs has yet been developed.

【００２１】また、前述の唯一のタバコスズメガ（蛾
類）の人工採卵方法の場合は、 タバコの生葉を乾燥状態で保存するために、そのメ
タノール抽出液を調製する際には大型のソクスレー抽出
器を用いて１〜２時間抽出操作を行なわねばならず、手
間がかかり、煩雑となりやすい。 採卵を行なう際に用いる抽出液は高濃度の抽出液
（生葉濃度375mg／ml＝375g／l）が必要なため、高濃度
の抽出液1l(1000cc)を得るのに生葉は375g必要であり、
しかも抽出液のメタノール濃度も80％と高いため蒸発量
が大きく、常に抽出液の補給が必要となって大量のタバ
コ生葉を必要とする。 メタノール濃度が80％と高く、かつ蒸発量が大きい
ために抽出液を連続補給することが出来ないので、毎日
採卵箱の消灯時間の２時間前にウレタンフォームへの抽
出液の入れ換えおよび採卵装置の設置し直し作業が必要
となる。 等の問題があるために、この採卵法はタバコスズメガの
屋内飼育現場には現実的には普及していない。In the case of the above-mentioned only artificial egg collection method for tobacco spider (moths), a large Soxhlet extractor is used when preparing a methanol extract to preserve fresh leaves of tobacco in a dry state. , The extraction operation must be performed for 1 to 2 hours, which is troublesome and complicated. Since the extract used for egg collection requires a high-concentration extract (375 mg / ml of fresh leaves = 375 g / l), 375 g of fresh leaves are required to obtain 1 liter (1000 cc) of a high-concentration extract.
Moreover, since the methanol concentration of the extract is as high as 80%, the amount of evaporation is large, and the replenishment of the extract is always required, and a large amount of fresh tobacco leaves is required. Since the concentration of methanol is as high as 80% and the amount of evaporation is large, it is not possible to continuously replenish the extract. Therefore, two hours before the turn-off time of the egg collection box, the extract should be replaced with urethane foam and the egg collection device should be replaced every day. Re-installation work is required. Due to these problems, this egg collection method has not been practically used in indoor breeding sites of tobacco sparrows.

【００２２】このように、現在のところ、蝶・蛾等の鱗
翅目昆虫の人工採卵法で実用化に至ったものはない。As described above, no artificial egg collection method for lepidopteran insects such as butterflies and moths has been put to practical use at present.

【００２３】本発明の目的は、屋外内において鉢植えの
鱗翅目昆虫の寄主植物の生葉を用い、この生葉に産卵さ
せ、これを採卵する場合と比較して全く遜色がなく、同
等の高収率で、かつ継続的に採卵を行なえる鱗翅目昆虫
の人工採卵法およびその人工採卵装置を提供することに
ある。An object of the present invention is to use the leaves of a host plant of a lepidopteran insect planted outdoors in the outdoors, lay eggs on the leaves, and compare them with the case of collecting eggs, and have the same high yield. Another object of the present invention is to provide an artificial egg collecting method and an artificial egg collecting apparatus for lepidopteran insects that can perform egg collection continuously.

【００２４】[0024]

【課題を解決するための手段】本発明の人工採卵法は、
板状本体の表面側に鱗翅目昆虫の寄主植物の生葉抽出原
液から得られた採卵液を保持する板状本体の少なくとも
裏面側で鱗翅目昆虫に産卵させた後、これを採卵するこ
とを特徴とする。The artificial egg collecting method of the present invention comprises:
After laying eggs on lepidopteran insects on at least the back side of the plate-shaped main body holding the egg collection liquid obtained from the raw leaf extract of the host plant of the lepidopteran insect on the front side of the plate-shaped main body, collecting the eggs And

【００２５】前記採卵液は板状本体の表面側に装着した
液保持部材に保持し、また、前記産卵および採卵は板状
本体の裏面側に装着した採卵部材で行なうようにしても
よい。The egg collecting liquid may be held by a liquid holding member mounted on the front side of the plate-shaped main body, and the egg laying and egg collection may be performed by an egg collecting member mounted on the back side of the plate-shaped main body.

【００２６】また、前記採卵液の生葉濃度は5mg／mlな
いし50mg／mlの範囲で行なうようにしてもよい。The concentration of fresh leaves in the egg collection solution may be in the range of 5 mg / ml to 50 mg / ml.

【００２７】本発明の人工採卵装置は、支柱の上端部に
着脱自在に装着した板状本体と、板状本体の上面側に着
脱自在に装着した鱗翅目昆虫の寄主植物の生葉抽出原液
から得られた採卵液を保持する液保持部材と、板状本体
の下面側に着脱自在に装着した鱗翅目昆虫の採卵を行な
う採卵部材とを有することを特徴とする。The artificial egg collecting apparatus of the present invention is obtained from a plate-shaped main body detachably mounted on the upper end of a support, and a raw leaf extract of a host plant of a Lepidoptera insect detachably mounted on the upper surface side of the plate-shaped main body. A liquid holding member for holding the obtained egg collecting liquid, and an egg collecting member for collecting eggs of lepidopteran insects detachably attached to the lower surface side of the plate-shaped main body.

【００２８】前記支柱はその下方に着脱自在の台座を有
し、その上方に採卵液を貯蔵する液貯蔵部を有し、該液
貯蔵部は前記液保持部材に採卵液を補給する液補給部材
を備えるようにしてもよい。The column has a detachable pedestal below it, and a liquid storage section for storing the egg collection liquid above it, and the liquid storage section is a liquid supply member for supplying the liquid holding member with the egg collection liquid. May be provided.

【００２９】また、前記液保持部材はその上方に液保持
部材より小径であって着脱自在の上蓋を備えるようにし
てもよい。Further, the liquid holding member may have a detachable upper lid having a smaller diameter than the liquid holding member above the liquid holding member.

【００３０】［作用］板状本体には鱗翅目昆虫の寄主植
物の生葉抽出原液から得られた採卵液を保持しているか
ら、板状本体から発散する採卵液に誘い出されて鱗翅目
昆虫が板状本体に飛来し、ドラミング行動またはホバリ
ング行動をとりながら産卵行動を誘発し、板状本体に産
卵する。そして、産み付けられた鱗翅目昆虫の卵は従来
の生葉に産卵させた卵のように１粒ずつ取り外すことな
く板状本体と共に回収が可能となり、卵に直接人手が触
れることがなく、採卵が容易に行なえる。[Effect] Since the plate-shaped body holds the egg-collecting solution obtained from the undiluted solution of the leaf extract of the host plant of the Lepidoptera insects, the plate-shaped body is attracted to the egg-collecting solution diverging from the plate-shaped body to produce the lepidoptera insects Flies to the plate-shaped body, induces an egg-laying behavior while taking a drumming action or a hovering action, and lays eggs on the plate-shaped body. And the eggs of the lepidopteran insects laid can be collected together with the plate-shaped body without removing one by one like eggs laid on conventional fresh leaves, and the eggs can be collected without direct human contact. Easy to do.

【００３１】また、板状本体に装着した産卵部材に産卵
させるときは、産み付けられた鱗翅目昆虫の卵は採卵部
材と共に回収することが出来て、採卵をより効果的に行
なえる。この場合、採卵部材の上で直接孵化させること
が出来る。When laying eggs on the egg-laying member attached to the plate-shaped main body, the laid eggs of the lepidopteran insects can be collected together with the egg-collecting member, so that the eggs can be collected more effectively. In this case, hatching can be performed directly on the egg collecting member.

【００３２】採卵液は鱗翅目昆虫の寄主植物の生葉抽出
原液から得られた液を用いるようにしたので、寄主植物
の生葉を栽培する必要がなく、また、採卵液の生葉抽出
原液は低温で長期間に亘って保存が可能である。As the egg collection solution, a solution obtained from a raw leaf extract of a host plant of a lepidopteran insect is used, so that it is not necessary to cultivate the leaf of the host plant. It can be stored for a long time.

【００３３】[0033]

【発明の実施の形態】鱗翅目昆虫の寄主植物の生葉の抽
出は、次の３通りの方法のうち採卵する鱗翅目昆虫の種
類に対応させて行なえばよい。BEST MODE FOR CARRYING OUT THE INVENTION Extraction of the leaves of a host plant of a Lepidoptera insect may be performed according to the type of Lepidoptera insect to be egg-collected in the following three methods.

【００３４】 寄主植物の生葉1kgに水を加えながらミ
キサーで摩砕しながら計3,200mlの水を加えて、均一に
なるように１〜２分間摩砕し、ガーゼと濾紙で濾過後、
濾過液を生葉抽出原液として温度−20℃で保存する。[0034] A total of 3,200 ml of water is added to 1 kg of the fresh leaves of the host plant while adding water while grinding with a mixer, and the mixture is uniformly ground for 1 to 2 minutes, and filtered with gauze and filter paper.
The filtrate is stored at a temperature of −20 ° C. as a raw leaf extract stock solution.

【００３５】 寄主植物の生葉1kgを幅1cm程度の短冊
状に切断し、これに抽出溶媒としてエタノール、または
メタノール3,200mlを加えて、密封出来る容器に入れ、
温度4℃で、5日間抽出し、ガーゼと濾紙で濾過後、濾過
液を生葉抽出原液として温度−20℃で保存する。ただ
し、生葉が抽出溶媒の上に浮上しやすいので、抽出中は
重りを乗せる必要がある。また、生葉からの均一な抽出
を図るために適宜に攪拌を行なう必要がある。[0035] 1 kg of fresh leaves of the host plant is cut into strips having a width of about 1 cm, and ethanol or methanol (3,200 ml) is added thereto as an extraction solvent, and put into a sealable container.
After extracting at a temperature of 4 ° C for 5 days and filtering with gauze and filter paper, the filtrate is stored at a temperature of -20 ° C as a raw leaf extract stock solution. However, it is necessary to add a weight during the extraction, because the fresh leaves are likely to float on the extraction solvent. In addition, it is necessary to perform appropriate stirring to achieve uniform extraction from fresh leaves.

【００３６】 寄主植物の生葉1kgに抽出溶媒としてエ
タノールまたはメタノールを加えながらミキサーで摩砕
しながら計3,200mlのエタノールまたはメタノールを加
えて、均一になるように１〜２分間摩砕し、ガーゼと濾
紙で濾過後、濾過液を生葉抽出原液として温度−20℃で
保存する。[0036] A total of 3,200 ml of ethanol or methanol is added to 1 kg of the fresh leaves of the host plant while adding ethanol or methanol as an extraction solvent and triturating with a mixer. After filtration through filter paper, the filtrate is stored at −20 ° C. as a raw leaf extract stock solution.

【００３７】前記、、のいずれの抽出方法でも、
採卵結果はほとんど変わらない。In any of the above extraction methods,
Egg collection results are almost unchanged.

【００３８】前記の抽出方法の場合は、生葉抽出原液
が保存時に−20℃で凍結してしまうので、使用に先立っ
て凍結融解処理、即ち解凍処理が必要である。In the case of the above-mentioned extraction method, the raw leaf extract stock solution is frozen at −20 ° C. during storage, so that a freeze-thaw treatment, that is, a thawing treatment is required before use.

【００３９】前記、の抽出方法の場合は不凍液の状
態で長期間保存することが出来るため簡便である。前記
、の抽出の場合は生葉の水分量を80％とすると、生
葉抽出原液の組成はエタノールまたはメタノールが約80
％、生葉濃度が約250mg／mlである。The above extraction method is convenient because it can be stored for a long time in the state of antifreeze. In the case of the above-mentioned extraction, if the water content of the fresh leaves is 80%, the composition of the raw leaf extract stock solution is about 80% ethanol or methanol.
%, Fresh leaf concentration is about 250 mg / ml.

【００４０】そして、採卵液の作成は前記、、の
いずれの方法で得られた生葉抽出原液を次のように希釈
して調製する。生葉濃度の調製の際に用いる希釈液は水
でもよいが、採卵液の変質や腐敗を防止するために、エ
タノールまたはメタノールの最終濃度15％溶液に調製す
ると採卵液は室温（25℃）で10日間失活しない。The egg collection solution is prepared by diluting the raw leaf extract stock solution obtained by any of the above methods as follows. The diluent used for preparing the fresh leaf concentration may be water, but if the final concentration of ethanol or methanol is adjusted to 15% in order to prevent deterioration and spoilage of the egg collection solution, the egg collection solution will be at room temperature (25 ° C). Does not expire for days.

【００４１】また、採卵液の生葉濃度範囲は、採卵する
鱗翅目昆虫の種類によるが、その下限は生葉濃度5mg／m
lで、また、上限は生葉濃度50mg／mlで鉢植えの寄主植
物の生葉とほぼ同程度の効果が得られる。The range of the fresh leaf concentration of the egg collection solution depends on the kind of lepidopteran insects to be collected, but the lower limit is 5 mg / m of the fresh leaf concentration.
1 and the upper limit is about 50 mg / ml of fresh leaf concentration, and almost the same effect as the fresh leaf of the potted host plant can be obtained.

【００４２】前述のように生葉抽出原液は低温で半永久
的に保存が可能であり、また、例えば生葉濃度が約250m
g／mlの生葉抽出原液の場合は生葉抽出原液を5〜50倍に
希釈したものを採卵液として用いるようにしたから、最
低で生葉を数十ｇ程度準備すれば一年間の採卵が可能で
ある。また、採卵液は変質・腐敗に対して強く、採卵時
には１週間以上交換する必要がない。As described above, the raw leaf extract stock solution can be stored semipermanently at a low temperature.
In the case of a fresh leaf extract solution of g / ml, a solution obtained by diluting the fresh leaf extract stock solution 5 to 50 times is used as an egg collection solution, so that a minimum of several tens g of fresh leaves can be used for one year of egg collection. is there. In addition, the egg collection liquid is resistant to deterioration and decay, and does not need to be replaced for one week or more when collecting eggs.

【００４３】本発明では極めて微量の採卵液で産卵行動
を誘発することが出来るため、産卵刺激物質の化学構造
を決定する過程での高感度バイオアッセイ系として有用
である。According to the present invention, since the spawning behavior can be induced with a very small amount of egg collection liquid, it is useful as a highly sensitive bioassay system in the process of determining the chemical structure of the spawning stimulating substance.

【００４４】[0044]

【実施例】先ず、本発明の鱗翅目昆虫の人工採卵装置に
ついて説明する。DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS First, an artificial egg collecting apparatus for lepidopteran insects according to the present invention will be described.

【００４５】実施例１ 図１ないし図３は本発明の鱗翅目昆虫の人工採卵法を実
施するための鱗翅目昆虫の人工採卵装置の１例を示すも
のであり、図中、１は人工採卵装置を示す。Example 1 FIGS. 1 to 3 show an example of an artificial egg collecting apparatus for lepidopteran insects for carrying out the artificial egg collecting method for lepidopteran insects according to the present invention. The device is shown.

【００４６】人工採卵装置１は、外径48mm、内径40mm、
高さ370mmの塩化ビニール樹脂製の筒状の支柱２と、上
面外径125mm、下面外径120mm、厚さ15mmの発泡スチロー
ル製の板状本体３と、産卵液を保持する径125mmのフィ
ルターペーパー（東洋ろ紙株式会社製）の液保持部材４
と、外径120mm、内径15mm、厚さ1.5mmのプラスチック製
のドウナツ状の採卵部材５とで構成されている。The artificial egg collecting apparatus 1 has an outer diameter of 48 mm, an inner diameter of 40 mm,
A cylindrical support 2 made of vinyl chloride resin having a height of 370 mm, a plate-shaped main body 3 made of styrofoam having an upper surface outer diameter of 125 mm, a lower surface outer diameter of 120 mm, and a thickness of 15 mm, and a filter paper of 125 mm in diameter for holding spawning liquid ( Liquid holding member 4 of Toyo Roshi Co., Ltd.)
And a plastic donut-shaped egg collecting member 5 having an outer diameter of 120 mm, an inner diameter of 15 mm, and a thickness of 1.5 mm.

【００４７】尚、板状本体３は図３（Ａ、Ｂ）示すよう
にその上面に幅15mm、深さ5mmの溝６を凹設し、また、
その中心に径15mmの孔７を穿設し、その孔７に塩化ビニ
ール樹脂製の外径15mm、内径9mm、長さ30mmの固定具８
を嵌挿した構成とした。As shown in FIGS. 3A and 3B, the plate-shaped main body 3 has a groove 6 having a width of 15 mm and a depth of 5 mm on its upper surface.
A hole 7 having a diameter of 15 mm is formed at the center thereof, and a fixing device 8 made of vinyl chloride resin having an outer diameter of 15 mm, an inner diameter of 9 mm, and a length of 30 mm is formed in the hole 7.
Was inserted.

【００４８】そして、図１に示す人工採卵装置１は次の
ように組み立てた。Then, the artificial egg collecting apparatus 1 shown in FIG. 1 was assembled as follows.

【００４９】先ず、支柱２の下方を底面外径125mm、厚
さ40mmの鉄合金製の台座９に、外径40mm、高さ30mmの鉄
合金製の取付け具１０を介して着脱自在に装着した。ま
た、支柱２の上方内に外径40mm、内径36mm、長さ130mm
のガラス製の採卵液を貯蔵する液貯蔵部１１を挿入し
た。First, the lower part of the column 2 was detachably mounted on an iron alloy pedestal 9 having a bottom surface outer diameter of 125 mm and a thickness of 40 mm via an iron alloy mounting tool 10 having an outer diameter of 40 mm and a height of 30 mm. . In addition, the outer diameter is 40mm, the inner diameter is 36mm, and the length is 130mm inside the upper part of the column 2.
The liquid storage part 11 for storing the glass egg collection liquid was inserted.

【００５０】また、板状本体３の下面側に固定具８に採
卵部材５を外径35mm、内径15mm、厚さ15mmの発泡スチロ
ール製の止め具１３を介して着脱自在に装着した。The egg collecting member 5 was detachably attached to the fixture 8 on the lower surface of the plate-shaped main body 3 via a styrofoam stopper 13 having an outer diameter of 35 mm, an inner diameter of 15 mm, and a thickness of 15 mm.

【００５１】続いて、支柱２の上端部に採卵部材５を装
着した板状本体３をその下面に取り付けた固定具８を介
して着脱自在に装着した。Subsequently, the plate-shaped main body 3 on which the egg collecting member 5 was mounted on the upper end of the column 2 was detachably mounted via the fixture 8 mounted on the lower surface thereof.

【００５２】また、太さ7mm、長さ300mmのガーゼ製でロ
ープ状の液補給部材１２の一端側を板状本体３の上面に
凹設した溝６内に挿入すると共に、液補給部材１２の他
端側を液貯蔵部１１内の採卵液内に浸漬し、これに採卵
液を浸透させ、これを灯芯のように吸い上げるようにし
た。One end of a rope-shaped liquid supply member 12 made of gauze having a thickness of 7 mm and a length of 300 mm is inserted into a groove 6 formed in the upper surface of the plate-shaped main body 3. The other end was immersed in the egg collection liquid in the liquid storage unit 11, and the egg collection liquid was permeated into the liquid and sucked up like a wick.

【００５３】次に、板状本体３の上面側に液保持部材４
を２枚重ね合わせて着脱自在に装着して、液補給部材１
２の他端側を液保持部材４に当接させて、液貯蔵部内１
１に貯蔵されている採卵液を液補給部材１２に吸い上げ
させ、これを介して液保持部材４に補給するようにし
た。Next, the liquid holding member 4 is provided on the upper surface side of the plate-shaped main body 3.
Are removably mounted on top of each other with two
2 is brought into contact with the liquid holding member 4 so that
The egg collection liquid stored in 1 is sucked up by the liquid replenishing member 12, and the liquid holding member 4 is replenished therethrough.

【００５４】また、液保持部材４の上面に自動車用のカ
ーペット製の直径100mm、厚さ5mmの上蓋１４を載置し
た。Further, on the upper surface of the liquid holding member 4, an upper cover 14 made of carpet for automobiles having a diameter of 100 mm and a thickness of 5 mm was placed.

【００５５】尚、前記実施例装置では支柱２、板状本体
３、液保持部材４、採卵部材５、上蓋１４を夫々の形状
を円形としたが、本発明はこれに限定されるものではな
く、採卵する鱗翅目昆虫の種類、その性質に合わせてそ
の形状を設定すればよく、例えば六角形、八角形、楕円
形が挙げられる。In the above embodiment, the support 2, the plate-like main body 3, the liquid holding member 4, the egg collecting member 5, and the upper lid 14 are each circular in shape, but the present invention is not limited to this. What is necessary is just to set the shape according to the kind of the lepidopteran insect which collects, and the property, for example, a hexagon, an octagon, and an ellipse.

【００５６】支柱２の高さは採卵する鱗翅目昆虫の種
類、その性質に合わせて設定すればよく、台座９を含め
て100〜500mm程度とする。The height of the column 2 may be set in accordance with the type and nature of the lepidopteran insects to be collected. The height of the column 2 including the pedestal 9 is about 100 to 500 mm.

【００５７】また、板状本体３の厚さを15mmとしたが、
本発明はこれに限定されるものではなく、採卵する鱗翅
目昆虫の種類、その性質に合わせてその厚さを設定すれ
ばよい。Although the thickness of the plate-shaped main body 3 is set to 15 mm,
The present invention is not limited to this, and the thickness may be set according to the type and nature of the lepidopteran insect to be collected.

【００５８】また、液保持部材４にフィルターペーパー
を用いたが、本発明はこれに限定されるものではなく、
採卵液を浸透し得て、徐々に滲出・発散させ得る材質で
あればよく、例えばウレタンフォーム、スポンジ、布地
が挙げられる。Although the filter paper is used for the liquid holding member 4, the present invention is not limited to this.
Any material can be used as long as it can penetrate the egg collection liquid and gradually exude or diffuse, and examples thereof include urethane foam, sponge, and cloth.

【００５９】また、採卵部材５にプラスチックを用いた
が、本発明はこれに限定されるものではなく、鱗翅目昆
虫が産卵しやすく、かつ産卵後は容易に離脱し得ない材
質であればよく、例えば硬質紙板、鉄板、木板が挙げら
れる。採卵部材５はプラスチック材を用いれば強度的に
は半永久的であり、反復して使用することが出来るた
め、採卵部材５は７枚程度あれば１年間毎日連続して産
卵および採卵が出来る。Although plastic is used for the egg collecting member 5, the present invention is not limited to this, and any material may be used as long as it is easy for lepidopteran eggs to lay eggs and cannot be easily separated after laying eggs. Examples thereof include a hard paper plate, an iron plate, and a wooden plate. If the egg collecting member 5 is made of a plastic material, it is semi-permanent in terms of strength and can be used repeatedly. Therefore, if about 7 pieces of the egg collecting member 5 are used, they can lay eggs and collect eggs continuously for one year every day.

【００６０】前述のように、一般に蝶、蛾等の鱗翅目昆
虫は寄主植物の葉の表面側、即ち上面側で自己の寄主植
物であるかを認識し、葉の裏面側、即ち下面側に産卵す
る性質を持っている。このことから、本発明の鱗翅目昆
虫の人工採卵装置の構造は板状本体の上面側に装着した
液保持部材より寄主植物の生葉抽出原液から得られた採
卵液を滲出・発散させ、その採卵液に誘いだされた鱗翅
目昆虫は板状本体にドラミング行動またはホバリング行
動をしながら、その下面側に設置した採卵部材に産卵さ
せ、これを採卵するようにしている。As described above, in general, lepidopteran insects such as butterflies and moths recognize whether or not they are their own host plants on the front side of the leaves of the host plant, that is, on the upper side, and on the back side of the leaves, that is, on the lower side. It has the property of laying eggs. From this, the structure of the artificial egg collecting apparatus for lepidopteran insects of the present invention is such that the egg collecting liquid obtained from the raw leaf extract stock solution of the host plant is exuded and diverged from the liquid holding member attached to the upper surface side of the plate-shaped body, and the egg collection is performed. The lepidopteran insects induced by the liquid perform a drumming action or a hovering action on the plate-shaped main body, and lay eggs on an egg-collecting member provided on the lower surface side thereof to collect eggs.

【００６１】また、採卵液は経時と共に徐々に発散して
失われていくので、液貯蔵部１１内に採卵液、例えば約
150mlを貯蔵しておき、液補給部材１２を介して板状本
体３の上面に装着された液保持部材４に補給されるよう
になっている。Since the egg collection liquid gradually diverges and disappears over time, the egg collection liquid, for example, about
150 ml is stored, and supplied to the liquid holding member 4 mounted on the upper surface of the plate-shaped main body 3 via the liquid supply member 12.

【００６２】また、液保持部材４より小径の上蓋１４を
液保持部材４の上面に載置することにより、採卵液は液
保持部材４の縁周部のみから滲出・発散するようにな
る。また、採卵液の蒸発による損失を低減することが出
来る。また、産卵の際はホバリングしている鱗翅目昆虫
が掴まりやすくなる。Further, by placing the upper lid 14 having a smaller diameter than the liquid holding member 4 on the upper surface of the liquid holding member 4, the egg collection liquid is exuded and diverged only from the peripheral portion of the liquid holding member 4. In addition, loss due to evaporation of the egg collection liquid can be reduced. In addition, hovering lepidopteran insects can be easily grasped during spawning.

【００６３】そして、図４に示すように蝶・蛾等の鱗翅
目昆虫Ｇ、例えばエビガラスズメの場合は板状本体３の
縁周部をホバリング行動しながら、主に板状本体３の下
面側に装着した採卵部材５に卵Ｅを産卵する。As shown in FIG. 4, in the case of lepidopteran insects G such as butterflies and moths, for example, in the case of shrimp glass sparrows, the edge periphery of the plate-shaped main body 3 is hovered, and mainly on the lower surface side of the plate-shaped main body 3. An egg E is laid on the attached egg collecting member 5.

【００６４】板状本体３の下面側に装着した採卵部材５
には図５、図６に示すように卵Ｅが大量に産み付けられ
る。そして、産み付けられた卵Ｅは採卵部材５毎そのま
ま、板状本体３より取り外して回収し、必要に応じてホ
ルマリン等で消毒・殺菌処理を施した後、孵化・飼育さ
せることが出来るから、産み付けられた卵を、従来の寄
主植物の生葉に産卵させた卵のように１粒ずつ取り外す
必要性は全くない。また、採卵部材５毎シャーレに入れ
てシャーレ内での孵化・飼育が可能であり、鱗翅目昆虫
に人手が触れずに済む。また、採卵部材５毎シャーレに
入れて他部署へ搬送する際に卵の微生物汚染、変質を防
ぐことが出来る。Egg collecting member 5 mounted on the lower surface of plate-shaped main body 3
5 and 6, a large amount of eggs E are laid. Then, the laid eggs E can be removed from the plate-shaped main body 3 and collected as they are with the egg collecting members 5 as they are, and if necessary, disinfected and sterilized with formalin or the like, and then hatched and reared. There is no need to remove the laid eggs one by one as in the case of eggs laid on the leaves of conventional host plants. Further, the egg collecting member 5 can be hatched and bred in the petri dish by putting it in the petri dish, so that humans do not need to touch the lepidopteran insects. In addition, when the egg collecting member 5 is put into a petri dish and transported to another department, it is possible to prevent microbial contamination and deterioration of the egg.

【００６５】次に図１ないし図３に示す鱗翅目昆虫の人
工採卵装置１を用いて本発明の人工採卵法の具体的実施
例を対比例および比較例と共に説明する。尚、本発明の
具体的実施例および比較例は図７に示す採卵室内で産卵
および採卵を行い、また、対比例は図９に示す採卵室内
で産卵および採卵を行なった。Next, specific examples of the artificial egg collecting method of the present invention using the artificial egg collecting apparatus 1 for lepidopteran insects shown in FIGS. 1 to 3 will be described together with comparative examples and comparative examples. In the specific examples and comparative examples of the present invention, egg laying and egg collection were performed in the egg collection room shown in FIG. 7, and in contrast, egg laying and egg collection were performed in the egg collection room shown in FIG.

【００６６】本発明の具体的実施例および比較例に用い
る図７に示す採卵室の構成について説明する。図中、２
１は前面に開閉自在の扉を備える木製の箱(横幅73×奥
行85×高さ115cm)から成る採卵室を示す。The configuration of the egg collection room shown in FIG. 7 used in specific examples and comparative examples of the present invention will be described. In the figure, 2
Reference numeral 1 denotes an egg collection room composed of a wooden box (width 73 x depth 85 x height 115 cm) having a door that can be opened and closed on the front.

【００６７】採卵室２１の内部を艶消しの黒い紙で覆
い、タイマー２２で日長を16時間明、8時間暗(16L-8D)
のサイクルに制御した蛍光灯(15W)２３を設置し、ま
た、壁の上部にセンサー付き照明器具（5Wの電球）２４
を取り付け、夜間のみ採卵室の内部を微光照明した。ま
た、採卵室２１内の底部側の孔あき仕切板２５の下方に
水を入れた容器２６を設置し、更に室内全体の温度湿度
を制御する装置（図示せず）を設置して、室内温度を24
〜26℃、湿度を70〜80％RHに維持するようにした。ま
た、採卵室２１の上部にフィルター付き換気扇２７を配
置し、採卵室２１内で採卵および成虫の交尾による鱗粉
の舞い上がることを防止するようにした。また、採卵室
２１の前方側に羽化前の蛹を入れる籠２８を配置した。The inside of the egg collection room 21 is covered with matt black paper, and the day length is set to 16 hours light and 8 hours dark by a timer 22 (16L-8D).
Fluorescent lamp (15W) 23 controlled in the cycle of is installed, and lighting equipment with sensor (5W light bulb) 24
And the inside of the egg collection room was illuminated with low light only at night. In addition, a container 26 filled with water is installed below the perforated partition plate 25 on the bottom side in the egg collection room 21, and a device (not shown) for controlling the temperature and humidity of the entire room is installed. 24
The temperature was maintained at 、 26 ° C. and the humidity at 70-80% RH. In addition, a ventilation fan 27 with a filter was arranged in the upper part of the egg collection room 21 to prevent the flour from rising in the egg collection room 21 due to egg collection and adult mating. In addition, a basket 28 for putting pupae before emergence was arranged on the front side of the egg collection room 21.

【００６８】尚、本発明の人工採卵装置の構造は図７に
示す採卵室を含めた構造ではない。また、採卵室は採卵
を行なう鱗翅目昆虫の生態に合わせて照明、温度、湿度
等の基本的条件を満たす構造であればよく、図示例のよ
うな構成である必要は全くなく、例えば、単に段ボール
箱内に豆電球と本発明の採卵装置を設置しただけでも鱗
翅目昆虫の産卵と採卵は可能である。The structure of the artificial egg collecting apparatus of the present invention is not the structure including the egg collecting room shown in FIG. In addition, the egg collection room may be any structure that satisfies the basic conditions such as lighting, temperature, and humidity according to the ecology of the lepidopteran insect that performs egg collection, and does not need to have the configuration as shown in the example at all. Even if the miniature bulb and the egg collecting apparatus of the present invention are installed in a cardboard box, lepidopteran eggs can be laid and collected.

【００６９】実施例２ 本実施例はエビガラスズメ（蛾）の人工採卵法である。Example 2 This example is an artificial egg collection method for a shrimp turtle (moth).

【００７０】また、本実施例では採卵部材５に市販の直
径120mmのコンパクトディスク、また液貯蔵部１１に外
径40mm、内径36mm、長さ130mmのショウジョウバエ飼育
用ガラス管を利用した。また、板状本体３の側面全面に
亘って産卵された卵を取り外ししやすいように幅25mmの
ビニールテープ（住友スリーエム株式会社製）を貼着し
た。In this embodiment, a commercially available compact disc having a diameter of 120 mm was used for the egg collecting member 5, and a glass tube for breeding Drosophila having an outer diameter of 40 mm, an inner diameter of 36 mm, and a length of 130 mm was used for the liquid storage unit 11. Further, a 25 mm wide vinyl tape (manufactured by Sumitomo 3M Limited) was attached so that the eggs laid over the entire side surface of the plate-shaped main body 3 could be easily removed.

【００７１】先ず、エビガラスズメの寄主植物の生葉と
してサツマイモ生葉を１kg用意した。この生葉にエタノ
ールを加えながらミキサーで摩砕しながら、合計3,200m
lのエタノールを加えて、2分間摩砕した後、4℃で5日間
自然放置して、生葉成分を抽出した後、ガーゼと濾紙で
濾過し、濾過液を生葉濃度250mg／mlの生葉抽出原液と
し、これを温度−20℃で保存した。First, 1 kg of fresh leaves of sweet potato was prepared as fresh leaves of the host plant of the shrimp glass sparrow. While adding ethanol to the fresh leaves and grinding with a mixer, a total of 3,200 m
l of ethanol, triturated for 2 minutes, allowed to stand at 4 ° C for 5 days to extract the fresh leaf components, filtered through gauze and filter paper, and filtered the filtrate into a fresh leaf extract stock solution with a fresh leaf concentration of 250 mg / ml. This was stored at a temperature of -20 ° C.

【００７２】そして、実験開始前に保存された生葉濃度
250mg／mlの生葉抽出原液25mlに水100mlを加えて生葉濃
度50mg／mlの採卵液（5倍希釈）を作成した。Then, the fresh leaf concentration stored before the start of the experiment
100 ml of water was added to 25 ml of a 250 mg / ml stock extract of fresh leaves to prepare an egg collection solution (5-fold dilution) having a fresh leaf concentration of 50 mg / ml.

【００７３】また、液保持部材４を2枚板状本体３の上
面側に装着すると共に、生葉濃度50mg／mlの採卵液150m
lを液貯蔵部１１内に充填し、これを液補給部材１２に
浸透させ、液補給部材１２の毛細管現象で上方に吸い上
げて液保持部材４に補給するようにした。Further, the liquid holding member 4 is mounted on the upper surface side of the two plate-shaped main bodies 3 and an egg collection liquid 150 m / ml having a fresh leaf concentration of 50 mg / ml.
The liquid was filled in the liquid storage unit 11, penetrated into the liquid replenishing member 12, sucked upward by the capillary action of the liquid replenishing member 12, and supplied to the liquid holding member 4.

【００７４】また、採卵部材５を板状本体３の下面側に
装着した。The egg collecting member 5 was attached to the lower surface of the plate-shaped main body 3.

【００７５】次に、板状本体３に液保持部材４と採卵部
材５とを装着した鱗翅目昆虫の人工採卵装置１を前記図
７に示すように採卵室２１内に設置した。Next, the artificial egg collecting apparatus 1 for lepidopteran insects in which the liquid holding member 4 and the egg collecting member 5 were mounted on the plate-shaped main body 3 was set in the egg collecting room 21 as shown in FIG.

【００７６】そして、採卵室２１内に同一日に羽化した
エビガラスズメの雄５頭、雌５頭を放ち、全個体が死亡
するまで飼育、採卵を行なうと共に、交尾率、交尾雌１
頭の産卵数、産卵の集中度を調べ、その結果を図８に示
す。交尾率、交尾雌１頭の産卵数、産卵の集中度の結果
は全個体が死亡するまでの期間の合計とした。Then, five males and five females of the shrimp sparrow, which had emerged on the same day, were released into the egg collection room 21 and bred and collected until all the animals died.
The number of laying eggs and the concentration of laying eggs were examined, and the results are shown in FIG. The results of the mating rate, the number of laid eggs by one mated female, and the concentration of laying eggs were the total of the period until all the animals died.

【００７７】尚、交尾率、交尾雌１頭の産卵数、産卵の
集中度は次のようにして求めた。 交尾率（％）は飼育した雌に占める既交尾雌の割合
を表わす。 交尾雌１頭の産卵数（粒）は全採卵数を交尾雌の頭
数で割った値を表わす。 産卵の集中度（％）は全産卵数のうち、採卵装置の
板状本体の側面および採卵部材または寄主植物の生葉に
産下された卵の割合を表わす。The mating rate, the number of eggs laid per mating female, and the degree of concentration of eggs were determined as follows. Mating rate (%) represents the proportion of mated females to breeding females. The number of eggs laid per mating female (grain) represents a value obtained by dividing the total number of eggs collected by the number of mating females. The concentration (%) of the egg laying indicates the ratio of the eggs laid on the side surface of the plate-shaped main body of the egg collecting apparatus and the eggs of the egg collecting member or the host plant among the total number of eggs laid.

【００７８】実施例３ 採卵液として前記実施例２で作成された生葉濃度250mg
／mlの生葉抽出原液25mlに水207.5mlと、エタノール17.
5mlを加えて生葉濃度25mg／mlとした採卵液（10倍希
釈）を用いた以外は、前記実施例２と同様の方法で交尾
率、交尾雌１頭の産卵数、産卵の集中度を調べ、その結
果を図８に示す。Example 3 A fresh leaf concentration of 250 mg prepared in Example 2 was used as an egg collecting solution.
20 ml of water and 25 ml of ethanol 17.
Except for using an egg collection solution (10-fold dilution) with a fresh leaf concentration of 25 mg / ml by adding 5 ml, the mating rate, the number of eggs laid by one mated female, and the concentration of oviposition were examined in the same manner as in Example 2 above. FIG. 8 shows the results.

【００７９】実施例４ 採卵液として前記実施例２で作成された生葉濃度250mg
／mlの生葉抽出原液2.5mlに水105.8mlと、エタノール1
6.8mlを加えて生葉濃度5mg／mlとした採卵液（50倍希
釈）を用いた以外は、前記実施例２と同様の方法で交尾
率、交尾雌１頭の産卵数、産卵の集中度を調べ、その結
果を図８に示す。Example 4 A fresh leaf concentration of 250 mg prepared in Example 2 was used as an egg collection solution.
/5.8 ml of undiluted leaf extract solution, 105.8 ml of water and 1 part of ethanol
The mating rate, the number of eggs laid by one mated female, and the concentration of oviposition were determined in the same manner as in Example 2 except that an egg collection solution (50-fold dilution) was added to 6.8 ml to add a fresh leaf concentration of 5 mg / ml. The examination and the result are shown in FIG.

【００８０】対比例１ 採卵室として図９に示す従来法の生葉による採卵室を用
い、また、採卵液の代わりに鉢植えのサツマイモ（生葉
15〜20葉）を用いた以外は、前記実施例２と同様の方法
で交尾率、交尾雌１頭の産卵数、産卵の集中度を調べ、
その結果を図８に示す。Comparative Example 1 An egg collection room using fresh leaves according to the conventional method shown in FIG. 9 was used as an egg collection room, and a potted sweet potato (fresh leaves) was used instead of the egg collection solution.
15-20 leaves), except that the mating rate, the number of eggs laid by one mated female, and the degree of concentration of eggs were examined in the same manner as in Example 2 above.
FIG. 8 shows the result.

【００８１】比較例１ 採卵液として前記実施例２で作成された生葉濃度250mg
／mlの生葉抽出原液1mlに水212.3mlと、エタノール36.7
mlを加えて生葉濃度1mg／mlとした採卵液（250倍希釈）
を用いた以外は、前記実施例２と同様の方法で交尾率、
交尾雌１頭の産卵数、産卵の集中度を調べ、その結果を
図８に示す。Comparative Example 1 A fresh leaf concentration of 250 mg prepared in Example 2 was used as an egg collection liquid.
/ Ml fresh leaf extract solution (1 ml), water (212.3 ml) and ethanol (36.7)
Egg collection solution (250-fold dilution) with fresh leaf concentration of 1 mg / ml
Except that the mating rate was determined in the same manner as in Example 2;
The number of eggs laid and the concentration of eggs laid by one mating female were examined, and the results are shown in FIG.

【００８２】比較例２ 採卵液の代わりに１５％エタノール液（生葉濃度は0mg
／mlである）を用いた以外は、前記実施例２と同様の方
法で交尾率、交尾雌１頭の産卵数、産卵の集中度を調
べ、その結果を図８に示す。Comparative Example 2 A 15% ethanol solution (fresh leaf concentration was 0 mg
/ Ml), except that the mating rate, the number of eggs laid by one mated female and the concentration of eggs laid were examined in the same manner as in Example 2 above, and the results are shown in FIG.

【００８３】比較例３ 人工採卵装置１の代わりに図７に示す採卵室２１内にサ
ツマイモと同じ形状の造花葉を設置し、また、採卵室２
１内の造花葉近傍にに径125mmのフィルターペーパー
（東洋ろ紙株式会社製）を設置し、毎日消灯前に前記実
施例２で作成された生葉濃度250mg／mlの生葉抽出原液
を造花葉に10ml、フィルターペーパーに10mlを夫々吹き
かけた以外は、前記実施例２と同様の方法で交尾率、交
尾雌１頭の産卵数、産卵の集中度を調べ、その結果を図
８に示す。Comparative Example 3 Instead of the artificial egg collecting apparatus 1, artificial flower leaves having the same shape as the sweet potato were installed in the egg collecting chamber 21 shown in FIG.
A filter paper having a diameter of 125 mm (manufactured by Toyo Roshi Kabushiki Kaisha) was placed near the artificial flower leaf in 1, and 10 ml of the fresh leaf extract stock solution having a fresh leaf concentration of 250 mg / ml prepared in Example 2 was used every day before turning off the light. The mating rate, the number of eggs laid by one mated female, and the concentration of eggs laid were examined in the same manner as in Example 2 except that 10 ml of each was sprayed on the filter paper. The results are shown in FIG.

【００８４】図８から明らかなように、本発明の各実施
例の交尾率、交尾雌１当たりの産卵数および産卵の集中
度は、従来の鉢植えの寄主植物の生葉に産卵させて、こ
れを採卵する方法（対比例１）の交尾率、交尾雌１当た
りの産卵数および産卵の集中度と同等の効果が得られる
ことが確認された。As apparent from FIG. 8, the mating rate, the number of eggs laid per mated female, and the degree of concentration of eggs in each example of the present invention were determined by laying eggs on live leaves of a conventional potted host plant. It was confirmed that the same effects as the mating rate, the number of laid eggs per mated female, and the degree of concentration of the laid eggs were obtained by the method of collecting eggs (Comparative Example 1).

【００８５】また、採卵液を用いてもその生葉濃度が1m
g／ml（比較例１）の場合は本発明の各実施例のような
効果は得られない。Even when an egg collection solution is used, the fresh leaf concentration is 1 m.
In the case of g / ml (Comparative Example 1), the effect as in each example of the present invention cannot be obtained.

【００８６】また、採卵液を用いない（比較例２）場
合、造花葉に寄主植物の生葉抽出原液を吹きかけた（比
較例３）場合は、いずれも本発明の各実施例のような効
果は得られない。When no egg collection solution was used (Comparative Example 2) and when an undiluted solution of the leaf extract of the host plant was sprayed on the artificial leaves (Comparative Example 3), the effect of each of the examples of the present invention was not improved. I can't get it.

【００８７】従って、採卵液の生葉濃度は5mg／mlない
し50mg／mlの範囲内であれば十分に従来の鉢植えの寄主
植物の生葉を用いた場合と同等の効果が得られることが
確認された。Therefore, it was confirmed that if the fresh leaf concentration of the egg collection solution is in the range of 5 mg / ml to 50 mg / ml, the same effect as when using fresh leaves of the conventional potted host plant can be sufficiently obtained. .

【００８８】また、産卵刺激が不足すると、採卵装置の
板状本体の側面および採卵部材または寄主植物の生葉に
産卵せずに、採卵室の壁面、蛍光灯等に産卵してしま
い、卵が回収困難となる。If the stimulus for laying eggs is insufficient, the eggs are not laid on the side surfaces of the plate-shaped main body of the egg collecting apparatus and the egg collecting members or the leaves of the host plants, but are laid on the wall of the egg collecting room, fluorescent lamps, etc., and the eggs are collected. It will be difficult.

【００８９】実施例５ 本実施例はジャコウアゲハ（蝶）の人工採卵法である。Example 5 This example is an artificial egg collection method for Musk Swallowtail (butterfly).

【００９０】先ず、ジャコウアゲハの寄主植物の生葉と
してウマノスズクサ生葉を１kg用意した。この生葉にエ
タノールを加えながらミキサーで摩砕しながら、合計3,
200mlのエタノールを加えて、2分間摩砕した後、4℃で5
日間自然放置し、生葉成分を抽出した後、ガーゼと濾紙
で濾過し、濾過液を生葉濃度250mg／mlの生葉抽出原液
とし、これを温度−20℃で保存した。First, 1 kg of fresh leaves of Echinacea purpurea were prepared as fresh leaves of the host plant of Musk Swallowtail. While adding ethanol to the fresh leaves and grinding with a mixer, a total of 3,
After adding 200 ml of ethanol and triturating for 2 minutes,
After allowing to stand naturally for one day to extract fresh leaf components, the mixture was filtered through gauze and filter paper to obtain a filtrate as a fresh leaf extract stock solution having a fresh leaf concentration of 250 mg / ml, which was stored at a temperature of -20 ° C.

【００９１】そして、実験開始前に保存された生葉濃度
250mg／mlの生葉抽出原液25mlに水100mlを加えて生葉濃
度50mg／mlの採卵液（5倍希釈）を作成した。Then, the fresh leaf concentration stored before the start of the experiment
100 ml of water was added to 25 ml of a 250 mg / ml stock extract of fresh leaves to prepare an egg collection solution (5-fold dilution) having a fresh leaf concentration of 50 mg / ml.

【００９２】また、液保持部材４を2枚板状本体３の上
面側に装着すると共に、生葉濃度50mg／mlの採卵液150m
lを液貯蔵部１１内に充填し、これを液補給部材１２に
浸透させ、液補給部材１２の毛細管現象で上方に吸い上
げて液保持部材４に補給するようにした。Further, the liquid holding member 4 is mounted on the upper surface side of the two plate-shaped main bodies 3 and the egg collection liquid 150 m / ml having a fresh leaf concentration of 50 mg / ml.
The liquid was filled in the liquid storage unit 11, penetrated into the liquid replenishing member 12, sucked upward by the capillary action of the liquid replenishing member 12, and supplied to the liquid holding member 4.

【００９３】また、採卵部材５を板状本体３の下面側に
装着した。The egg collecting member 5 was mounted on the lower surface of the plate-shaped main body 3.

【００９４】次に、板状本体３に液保持部材４と採卵部
材５とを装着した鱗翅目昆虫の人工採卵装置１を前記図
７に示すように採卵室２１内に設置した。Next, the artificial egg collecting apparatus 1 for lepidopteran insects in which the liquid holding member 4 and the egg collecting member 5 were mounted on the plate-shaped main body 3 was set in the egg collecting room 21 as shown in FIG.

【００９５】そして、採卵室２１内に同一日に羽化した
ジャコウアゲハの雄２頭、雌２頭を放ち、全個体が死亡
するまで飼育、採卵を行なった。[0095] Two males and two females of the mud swallowtail, which emerged on the same day, were released into the egg collection room 21 and bred and collected until all the animals died.

【００９６】実施例６ 採卵液として前記実施例５で作成された生葉濃度250mg
／mlの生葉抽出原液25mlに水207.5mlと、エタノール17.
5mlを加えて生葉濃度25mg／mlとした採卵液（10倍希
釈）を用いた以外は、前記実施例５と同様の方法で全個
体が死亡するまで飼育、採卵を行なった。Example 6 Fresh leaf concentration of 250 mg prepared in Example 5 was used as an egg collection solution.
20 ml of water and 25 ml of ethanol 17.
Except for using an egg collection solution (10-fold dilution) with a fresh leaf concentration of 25 mg / ml by adding 5 ml, breeding and egg collection were performed until all individuals died in the same manner as in Example 5 above.

【００９７】対比例２ 採卵室として図９に示す従来法の生葉による採卵室を用
い、また、採卵液の代わりに鉢植えのウマノスズクサ
（生葉20〜30葉）を用いた以外は、前記実施例５と同様
の方法で全個体が死亡するまで飼育、採卵を行なった。Comparative Example 2 The same procedure as in Example 5 was carried out except that the egg collecting room using fresh leaves according to the conventional method shown in FIG. 9 was used as the egg collecting room, and a potted apricot clover (20 to 30 fresh leaves) was used instead of the egg collecting solution. Breeding and egg collection were carried out until all individuals died in the same manner as described above.

【００９８】比較例４ 採卵液の代わりに１５％エタノール液（生葉濃度は0mg
／mlである）を用いた以外は、前記実施例５と同様の方
法で全個体が死亡するまで飼育、採卵を行なった。Comparative Example 4 A 15% ethanol solution (the fresh leaf concentration was 0 mg
/ Ml), and bred and collected eggs until all individuals died in the same manner as in Example 5 above.

【００９９】実施例７ 生葉抽出原液としてサツマイモの生葉１kgに水を加えな
がらミキサーで摩砕しながら合計3,200mlの水を加え
て、2分間摩砕して生葉成分を抽出した後、ガーゼと濾
紙で濾過し、濾過液を温度−20℃で保存した生葉濃度25
0mg／mlの生葉抽出原液を用い、実験開始前に生葉抽出
原液に解凍処理を施した以外は、前記実施例２と同様の
方法でエビガラスズメ（蛾）の交尾率、交尾雌１頭の産
卵数、産卵の集中度を調べたところ、いずれも前記実施
例２と同じ結果が得られた。Example 7 A total of 3,200 ml of water was added to 1 kg of fresh leaves of sweet potato as a stock extract of fresh leaves while adding water and grinding with a mixer, followed by grinding for 2 minutes to extract fresh leaf components, and then gauze and filter paper. And the filtrate was stored at a temperature of −20 ° C.
The mating rate of shrimp viper (moth) and the number of eggs laid by one mating female in the same manner as in Example 2 except that the raw leaf extract stock solution was subjected to thawing treatment before starting the experiment, using 0 mg / ml fresh leaf extract stock solution. When the concentration of laying eggs was examined, the same results as in Example 2 were obtained.

【０１００】実施例８ 生葉抽出原液としてサツマイモの生葉１kgを幅1cmの短
冊状に切断し、これにエタノール3,200mlを加えて、密
封容器に入れ、温度4℃で、5日間静置して生葉成分を抽
出した後、ガーゼと濾紙で濾過し、濾過液を温度−20℃
で保存した生葉濃度250mg／mlの生葉抽出原液を用いた
以外は、前記実施例２と同様の方法でエビガラスズメ
（蛾）の交尾率、交尾雌１頭の産卵数、産卵の集中度を
調べたところ、いずれも前記実施例２と同じ結果が得ら
れた。Example 8 1 kg of fresh leaves of sweet potato was cut into 1 cm wide strips as an undiluted solution for extracting fresh leaves, and 3,200 ml of ethanol was added thereto. The resulting mixture was placed in a sealed container and allowed to stand at a temperature of 4 ° C. for 5 days. After extracting the components, the mixture was filtered through gauze and filter paper, and the filtrate was heated to -20 ° C.
Except for using the undiluted solution of fresh leaf extract having a fresh leaf concentration of 250 mg / ml, the mating rate of shrimp glass spider (moth), the number of eggs laid per mating female, and the degree of concentration of eggs were examined in the same manner as in Example 2 above. However, in each case, the same result as in Example 2 was obtained.

【０１０１】前述のように、本発明の鱗翅目昆虫の人工
採卵法は、年間を通じた寄主植物の栽培およびその管理
が必要なく、予め低温保存しておいた生葉抽出原液で作
成した採卵液で随時、採卵が行なえる。また、採卵装置
の設置とメンテナンスの操作も容易で、採卵装置の液保
持部材への採卵液の補充は１週間ないし10日に一度位の
頻度で行なえば十分である。As described above, the artificial egg collection method for lepidopteran insects of the present invention does not require cultivation and management of host plants throughout the year, and uses an egg collection liquid prepared from a raw leaf extract stock solution that has been stored at a low temperature in advance. Eggs can be collected at any time. Further, the operation of installing and maintaining the egg collecting apparatus is easy, and it is sufficient to replenish the liquid holding member of the egg collecting apparatus with the egg collecting liquid once every week or every ten days.

【０１０２】また、鱗翅目昆虫の採卵液に対する感度が
非常に高く、寄主植物の生葉が数十グラムあれば、一年
間連続して採卵が出来るシステムである。In addition, if the sensitivity of the lepidopteran insects to the egg collection solution is extremely high, and if there are several tens of grams of fresh leaves of the host plant, the system can continuously collect eggs for one year.

【０１０３】また、近年の蝶・蛾等の鱗翅目昆虫の遺伝
子組み換え技術が発達し、近い将来、遺伝子を組み換え
た鱗翅目昆虫を屋内で合理的に飼育する技術が必要にな
ると考えられる。Also, in recent years, techniques for genetically modifying lepidopteran insects such as butterflies and moths have been developed, and in the near future, a technique for rationally rearing genetically modified lepidopteran insects indoors will be required.

【０１０４】本発明の寄主植物の大量栽培、維持管理を
必要としない鱗翅目昆虫の人工採卵法および人工採卵装
置は、蝶、蛾等の鱗翅目昆虫の大量増殖と安定した飼育
維持を図る上で欠かせない手段であり、経済的な利点も
大きいものと考えられる。The artificial egg collecting method and artificial egg collecting apparatus for lepidopteran insects which do not require mass cultivation and maintenance of the host plant according to the present invention are intended for mass production of lepidopteran insects such as butterflies and moths and for stable breeding and maintenance. It is an indispensable means, and the economic advantage is considered to be great.

【０１０５】即ち、本発明の鱗翅目昆虫の人工採卵法の
意義は、第一に鱗翅目昆虫の寄主植物の生葉を用いずに
採卵出来ることにある。従来は、鱗翅目昆虫を飼育、採
卵するためには、寄主植物（アゲハチョウの場合は蜜柑
の木）を畑で栽培したり、鉢植えとして温室等で大量に
栽培する必要がある。特に、冬期間の飼育、採卵は大変
であり、照明装置が設置された温室等で寄主植物を管理
栽培する必要があった。本発明は１年に数十グラム程度
の寄主植物の生葉を採取すればよく、少量の採卵液を準
備することで一年中、簡単に鱗翅目昆虫の産卵および採
卵を行なうことが出来る点に有効性がある。That is, the artificial egg collection method for lepidopteran insects of the present invention is firstly capable of collecting eggs without using the leaves of host plants of lepidopteran insects. Conventionally, in order to breed and collect lepidopteran insects, it is necessary to cultivate a host plant (a tangerine tree in the case of a swallowtail butterfly) in a field or cultivate a large amount in a greenhouse or the like as a potted plant. In particular, breeding and egg collection during the winter period is difficult, and it is necessary to manage and cultivate the host plant in a greenhouse or the like where a lighting device is installed. The present invention is based on the point that it is sufficient to collect fresh leaves of a host plant of about several tens of grams per year, and it is possible to easily lay and collect lepidopteran insects throughout the year by preparing a small amount of egg collection solution. There is effectiveness.

【０１０６】第二として、人工採卵装置の形態が重要で
あり、本発明の鱗翅目昆虫人工採卵装置は鱗翅目昆虫の
産卵行動に合致した設計がなされていることにある。ア
ゲハチョウ類では前脚で寄主植物の生葉の上面を叩き、
産卵刺激物質を感受し、葉の下面に卵を産み付ける。蛾
類でも蝶類と同じ行動機構を持っているものと考えら
れ、種々の実験の結果、前記実施例のように液保持部材
から発散した採卵液に誘発され、産卵行動を起こし採卵
部材に産卵し、卵を採卵部材毎、そのまま回収すること
が可能となった。Second, the form of the artificial egg collecting apparatus is important, and the artificial egg collecting apparatus for lepidopteran insects of the present invention is designed to match the egg-laying behavior of lepidopteran insects. In swallowtail butterflies, the front leg hits the upper surface of the leaf of the host plant,
Sensitive to spawning stimulants and lays eggs on the underside of the leaves. It is considered that moths also have the same behavioral mechanism as butterflies.As a result of various experiments, the moths were induced by the egg collection liquid diverged from the liquid holding member as in the above-described example, and caused the egg-laying behavior to lay eggs on the egg collection member. Then, it became possible to collect the eggs as they were for each egg collecting member.

【０１０７】第三として、生葉抽出原液から得られた生
葉濃度（5mg／ml〜50mg／ml）の採卵液で産卵行動を誘
発出来る点にあることから、産卵刺激物質を精製、同定
する上でも有効であると言える。性フェロモン等と同様
に鱗翅目昆虫の寄主植物の活性成分についても害虫捕獲
法（防除）に利用することも可能性がある。更に本発明
の鱗翅目昆虫の人工採卵法および人工採卵装置は産卵刺
激物質と言ったような物質のバイオアッセイ系としても
利用することが出来るものと考えられる。Thirdly, since the spawning behavior can be induced by the egg collection solution having a fresh leaf concentration (5 mg / ml to 50 mg / ml) obtained from the undiluted solution of the fresh leaf extract, it is also necessary to purify and identify the spawning stimulating substance. It can be said that it is effective. Like the sex pheromone and the like, the active ingredient of the host plant of the order Lepidoptera may be used in the pest-trapping method (control). Further, it is considered that the artificial egg collecting method and artificial egg collecting apparatus for lepidopteran insects of the present invention can also be used as a bioassay system for a substance such as an egg-laying stimulating substance.

【０１０８】[0108]

【発明の効果】本発明の鱗翅目昆虫の人工採卵法による
ときは、板状本体には鱗翅目昆虫の寄主植物の生葉抽出
原液から得られた採卵液を有しているから、板状本体か
ら発散する採卵液に誘い出された鱗翅目昆虫が板状本体
に飛来し、ドラミング行動またはホバリング行動しなが
ら産卵行動を誘発し、板状本体に産卵し、これを採卵す
るようにしたので、産み付けられた鱗翅目昆虫の卵は従
来の生葉に産卵させた卵のように１粒ずつ取り外すこと
なく板状本体と共に回収が可能となり、採卵が容易に行
なえるので、極めて能率よく採卵出来る等の効果があ
る。According to the artificial egg collection method for lepidopteran insects of the present invention, since the plate-shaped body has an egg collection liquid obtained from a raw leaf extract of the host plant of the lepidopteran insect, the plate-shaped body As lepidopteran insects drawn to the egg collection fluid diverging from fly into the plate-shaped body, induce egg-laying behavior while drumming or hovering, lay eggs on the plate-shaped body, and collect eggs. The eggs of the lepidopteran insects laid can be collected together with the plate-shaped body without removing them one by one like the eggs laid on conventional fresh leaves, and the eggs can be collected easily, so the eggs can be collected extremely efficiently, etc. Has the effect.

【０１０９】また、板状本体の表面側の液保持部材に寄
主植物の生葉抽出原液から得られた採卵液を保持させる
ときは、採卵液の保持が容易であり、かつ、採卵液の補
充が容易となり、産卵および採卵が継続的に行なえる。When the liquid holding member on the surface side of the plate-shaped main body holds the egg collection liquid obtained from the raw leaf extract stock of the host plant, it is easy to hold the egg collection liquid and replenish the egg collection liquid. This makes it easier to lay eggs and collect eggs continuously.

【０１１０】また、鱗翅目昆虫の卵を板状本体の裏面側
の採卵部材に産み付けさせるときは、産み付けられた鱗
翅目昆虫の卵は従来の生葉に産卵させた卵のように１粒
ずつ取り外すことなく採卵部材と共に回収することが出
来るから、卵に直接人手が触れることなく採卵が容易に
行なえるので、卵は汚染されることがなく極めて能率よ
く採卵出来、また、卵は採卵部材と共に消毒が行なえ、
かつ採卵部材の上で孵化させることが可能となる。更
に、採卵部材の交換のみで産卵および採卵を継続的に行
なえる。When the eggs of lepidopteran insects are laid on the egg collecting member on the back side of the plate-shaped main body, one egg of the laid lepidopterous insects is similar to the egg laid on conventional fresh leaves. Since the eggs can be collected together with the egg collecting member without removing them one by one, the eggs can be easily collected without directly touching the eggs, so that the eggs can be collected extremely efficiently without being contaminated. Can be disinfected with
In addition, hatching can be performed on the egg collecting member. Further, the laying and collecting of eggs can be continuously performed only by replacing the egg collecting member.

【０１１１】また、採卵液は鱗翅目昆虫の寄主植物の生
葉抽出原液から得られた液を用いるようにしたので、生
葉抽出原液は低温で長期間に亘って保存が可能となり、
かつ採卵液は必要に応じて生葉抽出原液から作成すれば
よいので、変質・腐敗に対して強い。[0111] In addition, since the solution obtained from the undiluted solution of the leaf extract of the host plant of the order Lepidoptera is used as the egg collection solution, the undiluted solution of the leaf extract can be stored at a low temperature for a long period of time.
In addition, since the egg collection liquid may be prepared from the raw leaf extract as needed, it is resistant to deterioration and decay.

【０１１２】また、採卵液の生葉濃度は5mg／mlないし5
0mg／mlの範囲で、鱗翅目昆虫の寄主植物の生葉に直接
卵を産卵させ、これを採卵する従来法と同等の効果が得
られる。The concentration of fresh leaves in the egg collection solution is 5 mg / ml to 5 mg / ml.
Within the range of 0 mg / ml, an effect equivalent to that of the conventional method of directly laying eggs on the leaves of host plants of lepidopteran insects and collecting them can be obtained.

【０１１３】本発明の鱗翅目昆虫の人工採卵装置による
ときは、産卵された卵は採卵部材と共に回収が可能とな
り、卵に直接人手が触れることなく採卵が容易に行なえ
るので、卵は汚染されることがなく極めて能率よく採卵
出来る人工採卵装置を提供することが出来る。When the artificial egg collecting apparatus for lepidopteran insects of the present invention is used, the laid eggs can be collected together with the egg collecting member, and the eggs can be easily collected without directly touching the eggs. It is possible to provide an artificial egg collecting apparatus capable of collecting eggs very efficiently without any need for the method.

【０１１４】また、支柱の上方に採卵液を貯蔵する液貯
蔵部を備え、液貯蔵部と液保持部材との間に液補給部材
を備えるときは、液保持部材の採卵液が低減した時点で
採卵液は液貯蔵部より液補給部材を介して液保持部材に
補給することが出来る。Further, when a liquid storage section for storing the egg collection liquid is provided above the support and a liquid supply member is provided between the liquid storage section and the liquid holding member, when the amount of the egg collection liquid in the liquid holding member decreases, The egg collection liquid can be supplied from the liquid storage unit to the liquid holding member via the liquid supply member.

【０１１５】液保持部材の上方に液保持部材より小径の
上蓋を備えるときは、採卵液は液保持部材の周縁部のみ
から発散するので、採卵液の発散を効果的に出来ると共
に、採卵液の消費を低減することが出来る。When an upper lid having a smaller diameter than the liquid holding member is provided above the liquid holding member, the egg collection liquid diverges only from the peripheral portion of the liquid holding member, so that the dispersion of the egg collection liquid can be made effective and the egg collection liquid can be effectively dispersed. Consumption can be reduced.

【図面の簡単な説明】[Brief description of the drawings]

【図１】 本発明の鱗翅目昆虫の人工採卵装置の１実施
例の斜視図、FIG. 1 is a perspective view of an embodiment of an artificial egg collecting apparatus for lepidopteran insects according to the present invention,

【図２】 図１の人工採卵装置の各部品の説明図、FIG. 2 is an explanatory view of each part of the artificial egg collecting apparatus of FIG. 1,

【図３】 人工採卵装置に用いる板状本体の１例であ
り、（Ａ）はその平面図、（Ｂ）は（Ａ）のＢ−Ｂ線截
断面図、FIG. 3 is an example of a plate-shaped main body used in the artificial egg collecting apparatus, (A) is a plan view thereof, (B) is a cross-sectional view taken along the line BB of (A),

【図４】 本発明の鱗翅目昆虫の人工採卵装置を用いた
蛾の産卵中の１例を表わす斜視図、FIG. 4 is a perspective view showing an example of spawning of a moth using the artificial lepidopterous egg collecting apparatus of the present invention,

【図５】 採卵部材に産下された卵の状態を示す図、FIG. 5 is a view showing a state of an egg laid on an egg collecting member;

【図６】 図５の要部の拡大図、FIG. 6 is an enlarged view of a main part of FIG. 5,

【図７】 図１の人工採卵装置を採卵室内に設置した状
態を示す概略図、FIG. 7 is a schematic view showing a state where the artificial egg collecting apparatus of FIG. 1 is installed in an egg collecting room;

【図８】 本発明の実施例、対比例および比較例の実験
結果を示すグラフ図、FIG. 8 is a graph showing experimental results of Examples, Comparative Examples and Comparative Examples of the present invention;

【図９】 従来法の鱗翅目昆虫の採卵室の概略図。FIG. 9 is a schematic diagram of a lepidopteran insect egg collection room according to a conventional method.

【図面の符号】 １ 人工採卵装置、 ２ 支柱、 ３ 板状
本体、４ 液保持部材、 ５ 採卵部材、 ６
溝、９ 台座、 １１ 液貯蔵部、 １２
液補給部材、１４ 上蓋、 Ｅ 卵、 Ｇ
鱗翅目昆虫。[Signs in the drawings] 1 artificial egg collecting apparatus, 2 columns, 3 plate-shaped body, 4 liquid holding member, 5 egg collecting member, 6
Groove, 9 pedestals, 11 liquid storage, 12
Liquid replenishing member, 14 top lid, E egg, G
Lepidoptera insects.

───────────────────────────────────────────────────── フロントページの続き (72)発明者 井上 尚 茨城県つくば市吾妻２−３−２ 708−509 ──────────────────────────────────────────────────続 き Continued on the front page (72) Inventor Takashi Inoue 2-3-2 Azuma, Tsukuba, Ibaraki 708-509

Claims (6)

【特許請求の範囲】[Claims] 【請求項１】 板状本体の表面側に鱗翅目昆虫の寄主植
物の生葉抽出原液から得られた採卵液を保持する板状本
体の少なくとも裏面側で鱗翅目昆虫に産卵させた後、こ
れを採卵することを特徴とする鱗翅目昆虫の人工採卵
法。Claims 1. A lepidopteran egg is laid on at least the back side of a plate-shaped body holding an egg collection solution obtained from a raw leaf extract of a host plant of a lepidopteran insect on the surface side of the plate-shaped body. An artificial egg collection method for lepidopteran insects, which comprises collecting eggs. 【請求項２】 前記採卵液は板状本体の表面側に装着し
た液保持部材に保持し、また、前記産卵および採卵は板
状本体の裏面側に装着した採卵部材で行なうことを特徴
とする請求項第１項に記載の鱗翅目昆虫の人工採卵法。2. The egg collection liquid is held by a liquid holding member attached to the front side of the plate-shaped main body, and the egg laying and egg collection are performed by an egg collection member mounted on the back side of the plate-shaped main body. The method for artificially collecting lepidopteran insects according to claim 1. 【請求項３】 前記採卵液の生葉濃度は5mg／mlないし5
0mg／mlであることを特徴とする請求項第１項または第
２項に記載の鱗翅目昆虫の人工採卵法。3. The fresh leaf concentration of the egg collection solution is 5 mg / ml to 5 mg / ml.
The method for artificially collecting lepidopteran insects according to claim 1 or 2, wherein the method is 0 mg / ml. 【請求項４】 支柱の上端部に着脱自在に装着した板状
本体と、板状本体の上面側に着脱自在に装着した鱗翅目
昆虫の寄主植物の生葉抽出原液から得られた採卵液を保
持する液保持部材と、板状本体の下面側に着脱自在に装
着した鱗翅目昆虫の採卵を行なう採卵部材とを有するこ
とを特徴とする鱗翅目昆虫の人工採卵装置。4. A plate-shaped body detachably attached to the upper end of a column, and an egg-collecting liquid obtained from an undiluted leaf extract of a host plant of a lepidopteran insect detachably attached to an upper surface of the plate-shaped body. An artificial egg collecting apparatus for lepidopteran insects, comprising: a liquid holding member for performing lepidopteran insect removal; and an egg collecting member detachably mounted on a lower surface side of the plate-shaped body for collecting eggs of lepidopteran insects. 【請求項５】 前記支柱はその下方に着脱自在の台座を
有し、その上方に採卵液を貯蔵する液貯蔵部を有し、該
液貯蔵部は前記液保持部材に採卵液を補給する液補給部
材を有していることを特徴とする請求項第４項に記載の
鱗翅目昆虫の人工採卵装置。5. The column has a detachable pedestal below it, and a liquid storage unit for storing an egg collection liquid above the pedestal, the liquid storage unit being a liquid for replenishing the liquid holding member with the egg collection liquid. The artificial egg collecting apparatus for lepidopteran insects according to claim 4, further comprising a supply member. 【請求項６】 前記液保持部材はその上方に液保持部材
より小径であって着脱自在の上蓋を有していることを特
徴とする請求項第４項または第５項に記載の鱗翅目昆虫
の人工採卵装置。6. The lepidopteran insect according to claim 4, wherein the liquid holding member has a detachable upper lid having a smaller diameter than the liquid holding member above the liquid holding member. Artificial egg collecting equipment.