CN109169960A - Aqueous enzymatic method prepares the formula and preparation method of vegetable protein and edible oil - Google Patents
Aqueous enzymatic method prepares the formula and preparation method of vegetable protein and edible oil Download PDFInfo
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- CN109169960A CN109169960A CN201811189078.7A CN201811189078A CN109169960A CN 109169960 A CN109169960 A CN 109169960A CN 201811189078 A CN201811189078 A CN 201811189078A CN 109169960 A CN109169960 A CN 109169960A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
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Abstract
A kind of aqueous enzymatic method prepares the formula and preparation method of vegetable protein and edible oil, and aqueous enzymatic method prepares the formula of vegetable protein and edible oil, and the formula material includes: raw material one and raw material two；The raw material one is used to prepare sesame oil and high-quality sesame protein, the raw material one includes the water and complex cellulase of decortication sesame, raw material one, the solid-to-liquid ratio of the water of the decortication sesame and raw material one is 1: 8, and the weight percent that the complex cellulase accounts for the total weight of the water of the decortication sesame and raw material one is 0.4%；The raw material two is used to prepare edible protein.The sesame protein also has it is easy to appear the rotten, sesame protein and is difficult to by gastric juice decomposition its nutritional ingredient is difficult to by the defect of human intake when effectively preventing temperature raising in the prior art in conjunction with its method.
Description
Technical field
The present invention relates to aqueous enzymatic method technical fields, and in particular to a kind of aqueous enzymatic method prepares the formula of vegetable protein and edible oil
And preparation method.
Background technique
Grease preparation method has squeezing method, water substitution and lixiviation process, wherein it is leaching that current oil prodution industry is most widely used
Method out.Since the nineties, the research of oil and albumen becomes the hot spot paid close attention to both at home and abroad in enzyme process separation oil plant, currently, aqueous enzymatic method
Have become a kind of emerging oil extracting methods, it is the cell wall that vegetable oil material cell is acted on using hydrolase, makes its rupture
It degrades, the oil droplet in cell releases and assembles a kind of method for obtaining grease in water phase.
In this way for sesame come by hydrolysis Enzymatic Extraction sesame oil and protein concrete technology method be divided into alkali carries and
Two stages are digested, are now described below process, first decortication sesame grinding is slurried, weighs the sesame pulpous state of certain mass
Object is increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjusts pH value to 8.7, after stirring in water bath about 90min, adjusts pH value to 4.8
~5.2,0.4% complex cellulase is added.After 140~170min of enzyme hydrolysis enzymolysis time, centrifuge separation, since sedimentation is
Several difference is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom, by edible vegetable oil and emulsion layer, 85 DEG C of demulsifications
It is centrifuged after 20min, just obtains sesame oil of the yield 90% or so；By protein hydrolyzate tune pH value to protein solution isoelectric point
It is centrifuged after 4.5, protein precipitation, is collected after freeze-drying, obtain high-quality sesame protein.
This method can prepare sesame oil and protein simultaneously, and energy consumption is small, pollution-free, and investment goods are simple, this method institute
Preparing sesame oil, not only recovery rate is high, and quality reaches the standard of country-level sesame oil, and can be by all sesame proteins
It extracts, protein is hardly denaturalized, and has reached the comprehensive utilization of Sesame germplasm.
The sesame protein obtained in this way is generally used as edible protein in actual use, however in practice process
In, it is easy to appear going bad, in addition the sesame protein also has is difficult to by gastric juice point the sesame protein when the temperature increases
The shortcomings that solving and its nutritional ingredient be difficult to by human intake.
Summary of the invention
To solve the above problems, the present invention provides formula and preparations that a kind of aqueous enzymatic method prepares vegetable protein and edible oil
Method effectively prevents sesame protein when temperature raising in the prior art it is easy to appear the rotten, sesame proteins also
Be difficult to by gastric juice decompose and its nutritional ingredient is difficult to by the defect of human intake.
A kind of aqueous enzymatic method prepares vegetable protein and edible oil in order to overcome the deficiencies in the prior art, the present invention provides
The solution of formula and preparation method, specific as follows:
A kind of aqueous enzymatic method prepares the formula of vegetable protein and edible oil, and the formula material includes: raw material one and raw material two；
The raw material one is used to prepare sesame oil and high-quality sesame protein, and the raw material one includes decortication sesame, raw material
The solid-to-liquid ratio of the water of one water and complex cellulase, the decortication sesame and raw material one is 1: 8, and the complex cellulase accounts for
The weight percent of the total weight of the water of the decortication sesame and raw material one is 0.4%；
The raw material two is used to prepare edible protein, and the raw material two includes the high-quality sesame that parts by weight are 460-570 parts
Increasing flesh powder that numb protein, parts by weight are 6-18 parts, the white granulated sugar that parts by weight are 48-64 parts, parts by weight are 18-34 parts
Mixture one, the Linseed oil that parts by weight are 3-25 parts, the rapeseed oil that parts by weight are 0.4-0.8 parts, parts by weight be
3-27 parts of soft white sugar, the arabinose that parts by weight are 0.3-0.5 parts, the heme iron that parts by weight are 0.07-0.25 parts,
Filefish that parts by weight are 0.7-2.3 parts oil, the deep sea fish oil that parts by weight are 0.7-2.3 parts, parts by weight are 0.6-1.8 parts
Lactein, the mixture two that parts by weight are 0.22-0.48 parts, the mixture two that parts by weight are 490-500 parts water
And the mixture three that parts by weight are 0.24-0.38 parts.
The mixture is second is that amino-acid zinc, ferric pyrophosphate and magnesium stearate mix obtained, the amino-acid zinc, burnt phosphorus thoroughly
The weight ratio of sour iron and magnesium stearate is 0.8:52:72.
The mixture is third is that orotic acid, pangamic acid, natural tocopherol and linolenic acid mix obtained, the whey thoroughly
Acid, pangamic acid, natural tocopherol and linolenic acid weight ratio be 172:370:0.78:7.4.
The mixture is first is that Semen setariae oil, cottonseed oil and camellia oil mix obtained, the Semen setariae oil, cottonseed oil and camellia thoroughly
The weight ratio of oil is 1:2:1.
The aqueous enzymatic method prepares the preparation method of vegetable protein and edible oil, includes the following steps:
Step a, described by hydrolyzing Enzymatic Extraction sesame oil first by hydrolysis Enzymatic Extraction sesame oil and protein
It is divided into alkali carries and two stages of enzymatic hydrolysis with the concrete technology method of protein, i.e., first decortication sesame grinding is slurried, is weighed certain
The sesame slurry of quality is increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjusts pH value to 8.7, stirring in water bath about 90min
Afterwards, it adjusts pH value to 4.8~5.2,0.4% complex cellulase is added.After 140~170min of enzyme hydrolysis enzymolysis time, centrifugation
Separation is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom due to the difference of sedimentation coefficient, by edible vegetable oil and
Emulsion layer is centrifuged after 85 DEG C of demulsification 20min, just obtains sesame oil of the yield 90% or so；Protein hydrolyzate tune pH value is arrived
It is centrifuged after protein solution isoelectric point 4.5, protein precipitation, is collected after freeze-drying, obtain high-quality sesame protein；
Step b, it mixes, the mixing is exactly that the high-quality sesame protein that parts by weight are 460-570 parts is put into
In glass container, the component after mixing thoroughly is then poured into glass container and is mixed thoroughly with column agitator arm；
Step c, it heats, the heating is exactly the component for carrying out heating to glass container and removing moisture removal to allow in glass container
Solution concentration percentage be 20%-25%, thus obtained intermediate products；
Step d, then pouring increasing flesh powder, parts by weight that parts by weight are 6-18 parts into the glass container again is
0.07-0.25 parts of heme iron, the filefish that parts by weight are 0.7-2.3 parts oil, the deep-sea fish that parts by weight are 0.7-2.3 parts
It is mixed thoroughly after the lactein that oil and parts by weight are 0.6-1.8 parts, edible protein is obtained with regard to drying after mixing thoroughly.
It is described mix thoroughly after the mode of mixing thoroughly of component be: parts by weight be 48-64 parts white granulated sugar, parts by weight 3-
27 parts of soft white sugar, the arabinose that parts by weight are 0.3-0.5 parts and the mixture two that parts by weight are 150-200 parts exist
It is mixed thoroughly in ceramic vessel one；Parts by weight be 18-34 parts mixture one, the Linseed oil that parts by weight are 3-25 parts and
It is mixed thoroughly in the rapeseed oil stacked ceramic container two that parts by weight are 0.4-0.8 parts；The mixture that parts by weight are 0.22-0.48 parts
Two, the mixture three that the water for the mixture two that parts by weight are 240-350 parts and parts by weight are 0.24-0.38 parts pours pottery into
It mixes thoroughly in porcelain container two, is constituted in this way in group material of the ceramic vessel after being mixed thoroughly in, ceramic vessel two and ceramic vessel three
Component after mixing thoroughly.
The invention has the benefit that
The edible protein being prepared can be decomposed efficiently by gastric juice and make its nutritional ingredient easily by human intake,
Immunity is improved, the nutritional ingredient of the edible protein is also similar to people's milk；Increase flesh powder via joined, also improves in temperature
It is easy to appear rotten problems for the sesame protein obtained merely when raising.
Detailed description of the invention
Fig. 1 is preparation method flow chart of the invention.
Specific embodiment
The present invention is described further below in conjunction with drawings and examples.
The aqueous enzymatic method of the invention prepares the formula of vegetable protein and edible oil, and the formula material includes: raw material one and original
Material two；
The raw material one is used to prepare sesame oil and high-quality sesame protein, and the raw material one includes decortication sesame, raw material
The solid-to-liquid ratio of the water of one water and complex cellulase, the decortication sesame and raw material one is 1: 8, and the complex cellulase accounts for
The weight percent of the total weight of the water of the decortication sesame and raw material one is 0.4%；
The raw material two is used to prepare edible protein, and the raw material two includes the high-quality sesame that parts by weight are 460-570 parts
Increasing flesh powder that numb protein, parts by weight are 6-18 parts, the white granulated sugar that parts by weight are 48-64 parts, parts by weight are 18-34 parts
Mixture one, the Linseed oil that parts by weight are 3-25 parts, the rapeseed oil that parts by weight are 0.4-0.8 parts, parts by weight be
3-27 parts of soft white sugar, the arabinose that parts by weight are 0.3-0.5 parts, the heme iron that parts by weight are 0.07-0.25 parts,
Filefish that parts by weight are 0.7-2.3 parts oil, the deep sea fish oil that parts by weight are 0.7-2.3 parts, parts by weight are 0.6-1.8 parts
Lactein, the mixture two that parts by weight are 0.22-0.48 parts, the mixture two that parts by weight are 490-500 parts water
And the mixture three that parts by weight are 0.24-0.38 parts.
The mixture is second is that amino-acid zinc, ferric pyrophosphate and magnesium stearate mix obtained, the amino-acid zinc, burnt phosphorus thoroughly
The weight ratio of sour iron and magnesium stearate is 0.8:52:72.
The mixture is third is that orotic acid, pangamic acid, natural tocopherol and linolenic acid mix obtained, the whey thoroughly
Acid, pangamic acid, natural tocopherol and linolenic acid weight ratio be 172:370:0.78:7.4.
The mixture is first is that Semen setariae oil, cottonseed oil and camellia oil mix obtained, the Semen setariae oil, cottonseed oil and camellia thoroughly
The weight ratio of oil is 1:2:1.
The aqueous enzymatic method prepares the preparation method of vegetable protein and edible oil, includes the following steps:
Step a, described by hydrolyzing Enzymatic Extraction sesame oil first by hydrolysis Enzymatic Extraction sesame oil and protein
It is divided into alkali carries and two stages of enzymatic hydrolysis with the concrete technology method of protein, i.e., first decortication sesame grinding is slurried, is weighed certain
The sesame slurry of quality is increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjusts pH value to 8.7, stirring in water bath about 90min
Afterwards, it adjusts pH value to 4.8~5.2,0.4% complex cellulase is added.After 140~170min of enzyme hydrolysis enzymolysis time, centrifugation
Separation is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom due to the difference of sedimentation coefficient, by edible vegetable oil and
Emulsion layer is centrifuged after 85 DEG C of demulsification 20min, just obtains sesame oil of the yield 90% or so；Protein hydrolyzate tune pH value is arrived
It is centrifuged after protein solution isoelectric point 4.5, protein precipitation, is collected after freeze-drying, obtain high-quality sesame protein；
Step b, it mixes, the mixing is exactly that the high-quality sesame protein that parts by weight are 460-570 parts is put into
In glass container, the component after mixing thoroughly is then poured into glass container and is mixed thoroughly with column agitator arm；
Step c, it heats, the heating is exactly the component for carrying out heating to glass container and removing moisture removal to allow in glass container
Solution concentration percentage be 20%-25%, thus obtained intermediate products；
Step d, then pouring increasing flesh powder, parts by weight that parts by weight are 6-18 parts into the glass container again is
0.07-0.25 parts of heme iron, the filefish that parts by weight are 0.7-2.3 parts oil, the deep-sea fish that parts by weight are 0.7-2.3 parts
It is mixed thoroughly after the lactein that oil and parts by weight are 0.6-1.8 parts, edible protein is obtained with regard to drying after mixing thoroughly.
It is described mix thoroughly after the mode of mixing thoroughly of component be: parts by weight be 48-64 parts white granulated sugar, parts by weight 3-
27 parts of soft white sugar, the arabinose that parts by weight are 0.3-0.5 parts and the mixture two that parts by weight are 150-200 parts
Water is mixed thoroughly in ceramic vessel one；Parts by weight be 18-34 parts mixture one, the Linseed oil that parts by weight are 3-25 parts
And parts by weight are to mix thoroughly in 0.4-0.8 parts of rapeseed oil stacked ceramic container two；Parts by weight be 0.22-0.48 part mix
The mixture three that object two, the water for the mixture two that parts by weight are 240-350 parts and parts by weight are 0.24-0.38 parts is closed to fall
Group material into being mixed thoroughly in ceramic vessel two, in this way in ceramic vessel after being mixed thoroughly in, ceramic vessel two and ceramic vessel three
Constitute the component after mixing thoroughly.
The edible protein being prepared can be decomposed efficiently by gastric juice and make its nutritional ingredient easily by human intake,
Immunity is improved, the nutritional ingredient of the edible protein is also similar to people's milk；
Increase flesh powder via joined, also improving the sesame protein that obtains merely when the temperature rises, it is easy to appear changes
The problem of matter.
Embodiment 1:
Aqueous enzymatic method prepares the formula of vegetable protein and edible oil, and the formula material includes: raw material one and raw material two；
The raw material one is used to prepare sesame oil and high-quality sesame protein, and the raw material one includes decortication sesame, raw material
The solid-to-liquid ratio of the water of one water and complex cellulase, the decortication sesame and raw material one is 1: 8, and the complex cellulase accounts for
The weight percent of the total weight of the water of the decortication sesame and raw material one is 0.4%；
The raw material two is used to prepare edible protein, and the raw material two includes the high-quality sesame egg that parts by weight are 460 parts
Increasing flesh powder that white matter, parts by weight are 6 parts, the white granulated sugar that parts by weight are 48 parts, the mixture one that parts by weight are 18 parts, again
Measure the Linseed oil that number is 3 parts, the rapeseed oil that parts by weight are 0.4 part, the soft white sugar that parts by weight are 3 parts, parts by weight
For 0.3 part of arabinose, parts by weight be 0.07 part heme iron, filefish that parts by weight are 0.7 part oil, parts by weight
For 0.7 part of deep sea fish oil, parts by weight be 0.6 part lactein, the mixture two that parts by weight are 0.22 part, parts by weight
The mixture three that number is 0.24 part for the water and parts by weight of 490 parts of mixture two.
The mixture is second is that amino-acid zinc, ferric pyrophosphate and magnesium stearate mix obtained, the amino-acid zinc, burnt phosphorus thoroughly
The weight ratio of sour iron and magnesium stearate is 0.8:52:72.
The mixture is third is that orotic acid, pangamic acid, natural tocopherol and linolenic acid mix obtained, the whey thoroughly
Acid, pangamic acid, natural tocopherol and linolenic acid weight ratio be 172:370:0.78:7.4.
The mixture is first is that Semen setariae oil, cottonseed oil and camellia oil mix obtained, the Semen setariae oil, cottonseed oil and camellia thoroughly
The weight ratio of oil is 1:2:1.
The aqueous enzymatic method prepares the preparation method of vegetable protein and edible oil, includes the following steps:
Step a, described by hydrolyzing Enzymatic Extraction sesame oil first by hydrolysis Enzymatic Extraction sesame oil and protein
It is divided into alkali carries and two stages of enzymatic hydrolysis with the concrete technology method of protein, i.e., first decortication sesame grinding is slurried, is weighed certain
The sesame slurry of quality is increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjusts pH value to 8.7, stirring in water bath about 90min
Afterwards, it adjusts pH value to 4.8,0.4% complex cellulase is added.After enzyme hydrolysis enzymolysis time 140min, centrifuge separation, due to heavy
The difference for dropping coefficient, is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom, by edible vegetable oil and emulsion layer, 85 DEG C
It is centrifuged after demulsification 20min, just obtains sesame oil of the yield 90% or so；Protein hydrolyzate tune pH value is arrived into protein solution etc.
It is centrifuged after electricity point 4.5, protein precipitation is collected after freeze-drying, obtains high-quality sesame protein；
Step b, it mixes, the mixing is exactly that the high-quality sesame protein that parts by weight are 460 parts is put into glass
In container, the component after mixing thoroughly is then poured into glass container and is mixed thoroughly with column agitator arm；
Step c, it heats, the heating is exactly the component for carrying out heating to glass container and removing moisture removal to allow in glass container
Solution concentration percentage be 20%, thus obtained intermediate products；
Step d, then pouring increasing flesh powder, parts by weight that parts by weight are 6 parts into the glass container again is 0.07 part
Heme iron, filefish that parts by weight are 0.7 part oil, the deep sea fish oil that parts by weight are 0.7 part and parts by weight be 0.6
It is mixed thoroughly after the lactein of part, edible protein is obtained with regard to drying after mixing thoroughly.
It is described mix thoroughly after the mode of mixing thoroughly of component be: parts by weight be 48 parts white granulated sugar, parts by weight be 3 parts
The water for the mixture two that the arabinose and parts by weight that soft white sugar, parts by weight are 0.3 part are 150 parts is in ceramic vessel one
In mix thoroughly；It is 0.4 part mixture one, the Linseed oil that parts by weight are 3 parts and the parts by weight that parts by weight are 18 parts
Rapeseed oil stacked ceramic container two in mix thoroughly；Mixture two that parts by weight are 0.22 part, the mixing that parts by weight are 340 parts
The water and parts by weight of object two are to mix thoroughly in 0.24 part of three stacked ceramic container two of mixture, in this way ceramic vessel one,
Group material after mixing thoroughly in ceramic vessel two and ceramic vessel three just constitutes the component after mixing thoroughly.
The edible protein being prepared can be decomposed efficiently by gastric juice and make its nutritional ingredient easily by human intake,
Immunity is improved, the nutritional ingredient of the edible protein is also similar to people's milk；
Increase flesh powder via joined, also improving the sesame protein that obtains merely when the temperature rises, it is easy to appear changes
The problem of matter.
In addition, the edible protein that the present embodiment is obtained is as test group, and high-quality sesame egg made from the prior art
White matter as a control group, respectively tests the protein digestibility of test group and control group, obtains the result of table 1:
Table 1
| Protein digestibility (%) | |
| Test group | 96 |
| Control group | 68 |
And the method for the prior art specifically: by hydrolysis Enzymatic Extraction sesame oil and protein, described passes through water
The concrete technology method of solution Enzymatic Extraction sesame oil and protein is divided into alkali carries and two stages of enzymatic hydrolysis, i.e., first grinds decortication sesame
Slurry is worn into, the sesame slurry of certain mass is weighed, by solid-to-liquid ratio 1: 8 plus water, is increased to 50 DEG C to temperature, adjusts pH value to 8.7,
After stirring in water bath about 90min, adjusts pH value to 4.8,0.4% complex cellulase is added.After enzyme hydrolysis enzymolysis time 140min,
Centrifuge separation is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom due to the difference of sedimentation coefficient, will be clear
Oil and emulsion layer are centrifuged after 85 DEG C of demulsification 20min, just obtain sesame oil of the yield 90% or so；By protein hydrolyzate tune pH
It is centrifuged after being worth protein solution isoelectric point 4.5, protein precipitation, is collected after freeze-drying, obtain high-quality sesame protein.
Seen from table 1, compared with the high-quality sesame protein of the prior art, the edible protein of the present embodiment is significantly improved
Protein digestibility.
Embodiment 2:
Aqueous enzymatic method prepares the formula of vegetable protein and edible oil, and the formula material includes: raw material one and raw material two；
The raw material one is used to prepare sesame oil and high-quality sesame protein, and the raw material one includes decortication sesame, raw material
The solid-to-liquid ratio of the water of one water and complex cellulase, the decortication sesame and raw material one is 1: 8, and the complex cellulase accounts for
The weight percent of the total weight of the water of the decortication sesame and raw material one is 0.4%；
The raw material two is used to prepare edible protein, and the raw material two includes the high-quality sesame egg that parts by weight are 515 parts
Increasing flesh powder that white matter, parts by weight are 12 parts, the white granulated sugar that parts by weight are 56 parts, the mixture one that parts by weight are 26 parts,
Linseed oil that parts by weight are 14 parts, the rapeseed oil that parts by weight are 0.6 part, the soft white sugar that parts by weight are 15 parts, weight
Arabinose that number is 0.4 part, the heme iron that parts by weight are 0.16 part, the filefish oil that parts by weight are 1.5 parts, weight
Deep sea fish oil that number is 1.5 parts, the lactein that parts by weight are 1.2 parts, the mixture two that parts by weight are 0.35 part, again
The mixture three that the water and parts by weight for the mixture two that amount number is 495 parts are 0.26 part.
The mixture is second is that amino-acid zinc, ferric pyrophosphate and magnesium stearate mix obtained, the amino-acid zinc, burnt phosphorus thoroughly
The weight ratio of sour iron and magnesium stearate is 0.8:52:72.
The mixture is third is that orotic acid, pangamic acid, natural tocopherol and linolenic acid mix obtained, the whey thoroughly
Acid, pangamic acid, natural tocopherol and linolenic acid weight ratio be 172:370:0.78:7.4.
The mixture is first is that Semen setariae oil, cottonseed oil and camellia oil mix obtained, the Semen setariae oil, cottonseed oil and camellia thoroughly
The weight ratio of oil is 1:2:1.
The aqueous enzymatic method prepares the preparation method of vegetable protein and edible oil, includes the following steps:
Step a, described by hydrolyzing Enzymatic Extraction sesame oil first by hydrolysis Enzymatic Extraction sesame oil and protein
It is divided into alkali carries and two stages of enzymatic hydrolysis with the concrete technology method of protein, i.e., first decortication sesame grinding is slurried, is weighed certain
The sesame slurry of quality is increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjusts pH value to 8.7, stirring in water bath about 90min
Afterwards, it adjusts pH value to 5.0,0.4% complex cellulase is added.After enzyme hydrolysis enzymolysis time 155min, centrifuge separation, due to heavy
The difference for dropping coefficient, is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom, by edible vegetable oil and emulsion layer, 85 DEG C
It is centrifuged after demulsification 20min, just obtains sesame oil of the yield 90% or so；Protein hydrolyzate tune pH value is arrived into protein solution etc.
It is centrifuged after electricity point 4.5, protein precipitation is collected after freeze-drying, obtains high-quality sesame protein；
Step b, it mixes, the mixing is exactly that the high-quality sesame protein that parts by weight are 515 parts is put into glass
In container, the component after mixing thoroughly is then poured into glass container and is mixed thoroughly with column agitator arm；
Step c, it heats, the heating is exactly the component for carrying out heating to glass container and removing moisture removal to allow in glass container
Solution concentration percentage be 22%, thus obtained intermediate products；
Step d, then pouring increasing flesh powder, parts by weight that parts by weight are 12 parts into the glass container again is 0.16 part
Heme iron, filefish that parts by weight are 1.5 parts oil, the deep sea fish oil that parts by weight are 1.5 parts and parts by weight be 1.2
It is mixed thoroughly after the lactein of part, edible protein is obtained with regard to drying after mixing thoroughly.
It is described mix thoroughly after the mode of mixing thoroughly of component be: parts by weight be 56 parts white granulated sugar, parts by weight be 15 parts
Soft white sugar, the arabinose that parts by weight are 0.4 part and the mixture two that parts by weight are 175 parts water in ceramic vessel
It is mixed thoroughly in one；It is 0.6 mixture one, the Linseed oil that parts by weight are 14 parts and the parts by weight that parts by weight are 26 parts
It is mixed thoroughly in the rapeseed oil stacked ceramic container two of part；Mixture two that parts by weight are 0.35 part, parts by weight are 320 parts mixed
Water and the parts by weight of object two are closed to mix thoroughly in 0.26 part of three stacked ceramic container two of mixture, in this way in ceramic vessel
Group material after mixing thoroughly in, ceramic vessel two and ceramic vessel three constitutes the component after mixing thoroughly.
The edible protein being prepared can be decomposed efficiently by gastric juice and make its nutritional ingredient easily by human intake,
Immunity is improved, the nutritional ingredient of the edible protein is also similar to people's milk；
Increase flesh powder via joined, also improving the sesame protein that obtains merely when the temperature rises, it is easy to appear changes
The problem of matter.
In addition, the edible protein that the present embodiment is obtained is as test group, and high-quality sesame egg made from the prior art
White matter as a control group, respectively tests the protein digestibility of test group and control group, obtains the result of table 2:
Table 2
| Protein digestibility (%) | |
| Test group | 98 |
| Control group | 67 |
And the method for the prior art specifically: by hydrolysis Enzymatic Extraction sesame oil and protein, described passes through water
The concrete technology method of solution Enzymatic Extraction sesame oil and protein is divided into alkali carries and two stages of enzymatic hydrolysis, i.e., first grinds decortication sesame
Slurry is worn into, the sesame slurry of certain mass is weighed, by solid-to-liquid ratio 1: 8 plus water, is increased to 50 DEG C to temperature, adjusts pH value to 8.7,
After stirring in water bath about 90min, adjusts pH value to 5.0,0.4% complex cellulase is added.After enzyme hydrolysis enzymolysis time 155min,
Centrifuge separation is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom due to the difference of sedimentation coefficient, will be clear
Oil and emulsion layer are centrifuged after 85 DEG C of demulsification 20min, just obtain sesame oil of the yield 90% or so；By protein hydrolyzate tune pH
It is centrifuged after being worth protein solution isoelectric point 4.5, protein precipitation, is collected after freeze-drying, obtain high-quality sesame protein.
As can be seen from Table 2, the edible protein of the present embodiment significantly improves compared with the high-quality sesame protein of the prior art
Protein digestibility.
Embodiment 3:
Aqueous enzymatic method prepares the formula of vegetable protein and edible oil, and the formula material includes: raw material one and raw material two；
The raw material one is used to prepare sesame oil and high-quality sesame protein, and the raw material one includes decortication sesame, raw material
The solid-to-liquid ratio of the water of one water and complex cellulase, the decortication sesame and raw material one is 1: 8, and the complex cellulase accounts for
The weight percent of the total weight of the water of the decortication sesame and raw material one is 0.4%；
The raw material two is used to prepare edible protein, and the raw material two includes the high-quality sesame egg that parts by weight are 570 parts
Increasing flesh powder that white matter, parts by weight are 18 parts, the white granulated sugar that parts by weight are 64 parts, the mixture one that parts by weight are 34 parts,
Linseed oil that parts by weight are 25 parts, the rapeseed oil that parts by weight are 0.8 part, the soft white sugar that parts by weight are 27 parts, weight
Arabinose that number is 0.5 part, the heme iron that parts by weight are 0.25 part, the filefish oil that parts by weight are 2.3 parts, weight
Deep sea fish oil that number is 2.3 parts, the lactein that parts by weight are 1.8 parts, the mixture two that parts by weight are 0.48 part, again
The mixture three that the water and parts by weight for the mixture two that amount number is 500 parts are 0.38 part.
The mixture is second is that amino-acid zinc, ferric pyrophosphate and magnesium stearate mix obtained, the amino-acid zinc, burnt phosphorus thoroughly
The weight ratio of sour iron and magnesium stearate is 0.8:52:72.
The mixture is third is that orotic acid, pangamic acid, natural tocopherol and linolenic acid mix obtained, the whey thoroughly
Acid, pangamic acid, natural tocopherol and linolenic acid weight ratio be 172:370:0.78:7.4.
The mixture is first is that Semen setariae oil, cottonseed oil and camellia oil mix obtained, the Semen setariae oil, cottonseed oil and camellia thoroughly
The weight ratio of oil is 1:2:1.
The aqueous enzymatic method prepares the preparation method of vegetable protein and edible oil, includes the following steps:
Step a, described by hydrolyzing Enzymatic Extraction sesame oil first by hydrolysis Enzymatic Extraction sesame oil and protein
It is divided into alkali carries and two stages of enzymatic hydrolysis with the concrete technology method of protein, i.e., first decortication sesame grinding is slurried, is weighed certain
The sesame slurry of quality is increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjusts pH value to 8.7, stirring in water bath about 90min
Afterwards, it adjusts pH value to 5.2,0.4% complex cellulase is added.After enzyme hydrolysis enzymolysis time 170min, centrifuge separation, due to heavy
The difference for dropping coefficient, is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom, by edible vegetable oil and emulsion layer, 85 DEG C
It is centrifuged after demulsification 20min, just obtains sesame oil of the yield 90% or so；Protein hydrolyzate tune pH value is arrived into protein solution etc.
It is centrifuged after electricity point 4.5, protein precipitation is collected after freeze-drying, obtains high-quality sesame protein；
Step b, it mixes, the mixing is exactly that the high-quality sesame protein that parts by weight are 570 parts is put into glass
In container, the component after mixing thoroughly is then poured into glass container and is mixed thoroughly with column agitator arm；
Step c, it heats, the heating is exactly the component for carrying out heating to glass container and removing moisture removal to allow in glass container
Solution concentration percentage be 25%, thus obtained intermediate products；
Step d, then pouring increasing flesh powder, parts by weight that parts by weight are 18 parts into the glass container again is 0.25 part
Heme iron, filefish that parts by weight are 2.3 parts oil, the deep sea fish oil that parts by weight are 2.3 parts and parts by weight be 1.8
It is mixed thoroughly after the lactein of part, edible protein is obtained with regard to drying after mixing thoroughly.
It is described mix thoroughly after the mode of mixing thoroughly of component be: parts by weight be 64 parts white granulated sugar, parts by weight be 27 parts
Soft white sugar, the arabinose that parts by weight are 0.5 part and the mixture two that parts by weight are 200 parts water in ceramic vessel
It is mixed thoroughly in one；It is 0.8 mixture one, the Linseed oil that parts by weight are 25 parts and the parts by weight that parts by weight are 34 parts
It is mixed thoroughly in the rapeseed oil stacked ceramic container two of part；Mixture two that parts by weight are 0.48 part, parts by weight are 300 parts mixed
Water and the parts by weight of object two are closed to mix thoroughly in 0.38 part of three stacked ceramic container two of mixture, in this way in ceramic vessel
Group material after mixing thoroughly in, ceramic vessel two and ceramic vessel three constitutes the component after mixing thoroughly.
The edible protein being prepared can be decomposed efficiently by gastric juice and make its nutritional ingredient easily by human intake,
Immunity is improved, the nutritional ingredient of the edible protein is also similar to people's milk；
Increase flesh powder via joined, also improving the sesame protein that obtains merely when the temperature rises, it is easy to appear changes
The problem of matter.
In addition, the edible protein that the present embodiment is obtained is as test group, and high-quality sesame egg made from the prior art
White matter as a control group, respectively tests the protein digestibility of test group and control group, obtains the result of table 3:
Table 3
| Protein digestibility (%) | |
| Test group | 99 |
| Control group | 71 |
And the method for the prior art specifically: by hydrolysis Enzymatic Extraction sesame oil and protein, described passes through water
The concrete technology method of solution Enzymatic Extraction sesame oil and protein is divided into alkali carries and two stages of enzymatic hydrolysis, i.e., first grinds decortication sesame
Slurry is worn into, the sesame slurry of certain mass is weighed, by solid-to-liquid ratio 1: 8 plus water, is increased to 50 DEG C to temperature, adjusts pH value to 8.7,
After stirring in water bath about 90min, adjusts pH value to 5.2,0.4% complex cellulase is added.After enzyme hydrolysis enzymolysis time 170min,
Centrifuge separation is successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom due to the difference of sedimentation coefficient, will be clear
Oil and emulsion layer are centrifuged after 85 DEG C of demulsification 20min, just obtain sesame oil of the yield 90% or so；By protein hydrolyzate tune pH
It is centrifuged after being worth protein solution isoelectric point 4.5, protein precipitation, is collected after freeze-drying, obtain high-quality sesame protein.
Seen from table 3, compared with the high-quality sesame protein of the prior art, the edible protein of the present embodiment is significantly improved
Protein digestibility.
The present invention is described in a manner of being illustrated with embodiment above, it will be understood by those of skill in the art that this
It is open to be not limited to embodiments described above, in the case of without departing from the scope of the present invention, it can make a variety of changes, change
And replacement.
Claims (6)
1. the formula that a kind of aqueous enzymatic method prepares vegetable protein and edible oil, which is characterized in that aqueous enzymatic method prepares vegetable protein and food
With the formula of oil, the formula material includes: raw material one and raw material two；
The raw material one is used to prepare sesame oil and high-quality sesame protein, and the raw material one includes decortication sesame, raw material one
The solid-to-liquid ratio of the water of water and complex cellulase, the decortication sesame and raw material one is 1: 8, and the complex cellulase accounts for described
The weight percent of the total weight of the water of decortication sesame and raw material one is 0.4%；
The raw material two is used to prepare edible protein, and the raw material two includes the high-quality sesame egg that parts by weight are 460-570 parts
Increasing flesh powder that white matter, parts by weight are 6-18 parts, the white granulated sugar that parts by weight are 48-64 parts, parts by weight are 18-34 parts mixed
Close object one, the Linseed oil that parts by weight are 3-25 parts, the rapeseed oil that parts by weight are 0.4-0.8 parts, parts by weight 3-27
Soft white sugar, the arabinose that parts by weight are 0.3-0.5 parts, the heme iron that parts by weight are 0.07-0.25 parts, weight of part
Filefish that number is 0.7-2.3 parts oil, the deep sea fish oil that parts by weight are 0.7-2.3 parts, the cream that parts by weight are 0.6-1.8 parts
The water of mixture two, the mixture two that parts by weight are 490-500 parts that sour rhzomorph, parts by weight are 0.22-0.48 parts and
The mixture three that parts by weight are 0.24-0.38 parts.
2. the formula that aqueous enzymatic method according to claim 1 prepares vegetable protein and edible oil, which is characterized in that described is mixed
Object is closed second is that amino-acid zinc, ferric pyrophosphate and magnesium stearate mix obtained, the amino-acid zinc, ferric pyrophosphate and magnesium stearate thoroughly
Weight ratio is 0.8:52:72.
3. the formula that aqueous enzymatic method according to claim 1 prepares vegetable protein and edible oil, which is characterized in that described is mixed
Object is closed third is that orotic acid, pangamic acid, natural tocopherol and linolenic acid mix obtained, the orotic acid, pangamic acid, Tocopherol natural thoroughly
The weight ratio of phenol and linolenic acid is 172:370:0.78:7.4.
4. the formula that aqueous enzymatic method according to claim 1 prepares vegetable protein and edible oil, which is characterized in that described is mixed
Object is closed first is that Semen setariae oil, cottonseed oil and camellia oil are mixed thoroughly obtained, the weight ratio of the Semen setariae oil, cottonseed oil and camellia oil is 1:
2:1。
5. the preparation method that aqueous enzymatic method according to claim 1 prepares vegetable protein and edible oil, which is characterized in that including
Following steps:
Step a, described to pass through hydrolysis Enzymatic Extraction sesame oil and egg first by hydrolysis Enzymatic Extraction sesame oil and protein
The concrete technology method of white matter is divided into alkali carries and two stages of enzymatic hydrolysis, i.e., first decortication sesame grinding is slurried, weighs certain mass
Sesame slurry be increased to 50 DEG C to temperature by solid-to-liquid ratio 1: 8 plus water, adjust pH value to 8.7, after stirring in water bath about 90min,
It adjusts pH value to 4.8~5.2,0.4% complex cellulase is added.After 140~170min of enzyme hydrolysis enzymolysis time, centrifugation point
From being successively edible vegetable oil, emulsion, protein hydrolyzate, sesame residues from top to bottom, by edible vegetable oil and cream due to the difference of sedimentation coefficient
Change layer, is centrifuged after 85 DEG C of demulsification 20min, just obtains sesame oil of the yield 90% or so；By protein hydrolyzate tune pH value to egg
It is centrifuged after white matter solution isoelectric point 4.5, protein precipitation is collected after freeze-drying, obtains high-quality sesame protein；
Step b, it mixes, the mixing is exactly that the high-quality sesame protein that parts by weight are 460-570 parts is put into glass
In container, the component after mixing thoroughly is then poured into glass container and is mixed thoroughly with column agitator arm；
Step c, it heats, the heating is exactly component that heating removes moisture removal to allow in glass container is carried out to glass container molten
Liquid percentage is 20%-25%, has thus obtained intermediate products；
Step d, the increasing flesh powder that parts by weight are 6-18 parts, parts by weight 0.07- are then poured into the glass container again
0.25 part of heme iron, the filefish that parts by weight are 0.7-2.3 parts oil, deep sea fish oil that parts by weight are 0.7-2.3 parts with
And parts by weight obtain edible protein with regard to drying after mixing thoroughly to mix thoroughly after 0.6-1.8 parts of lactein.
6. the preparation method that aqueous enzymatic method according to claim 5 prepares vegetable protein and edible oil, which is characterized in that described
The mode of mixing thoroughly of component after mixing thoroughly is: parts by weight be 48-64 parts white granulated sugar, parts by weight be 3-27 parts silk floss it is white
Sugar, the arabinose that parts by weight are 0.3-0.5 parts and the mixture two that parts by weight are 150-200 parts are in ceramic vessel one
In mix thoroughly；It is mixture one, the Linseed oil that parts by weight are 3-25 parts and the parts by weight that parts by weight are 18-34 parts
It is mixed thoroughly in 0.4-0.8 parts of rapeseed oil stacked ceramic container two；Parts by weight be 0.22-0.48 parts mixture two, parts by weight
The three stacked ceramic container two of mixture that the water and parts by weight for the mixture two that number is 240-350 parts are 0.24-0.38 parts
In mix thoroughly, in this way after group material of the ceramic vessel after mixing thoroughly in, ceramic vessel two and ceramic vessel three is constituted and mixed thoroughly
Component.
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Application publication date: 20190111 |