CN109106738A

CN109106738A - A kind of nervonic acid composition and its application in neuroprotection

Info

Publication number: CN109106738A
Application number: CN201811066579.6A
Authority: CN
Inventors: 崔晓廷; 王悦
Original assignee: Shenyang Gaoming Pharmaceutical Technology Co Ltd
Current assignee: Shenyang Gaoming Pharmaceutical Technology Co Ltd
Priority date: 2018-09-12
Filing date: 2018-09-12
Publication date: 2019-01-01

Abstract

A kind of application the present invention provides nervonic acid composition and its in neuroprotection, belongs to field of medicaments.Nervonic acid, phosphatidylserine, DHA, celery oil, Chinese medical extract, nutrient prime replenisher are carried out organic complex by monarch principle by nervonic acid composition, composition is set to summarize neurotrophic agent, nerve fibre renovation agent, the activator of neurotransmitter, neural energy ensures many mechanism of agent, to which multisystem, multipath improve the permeability of neuron membrane, promote the release of neurotransmitter, the conduction of accelerans pulse, repair dredging nerve information channel, activation or reverse cerebral function.Nervonic acid composition provided by the invention breaches the limitation and deficiency that a certain ingredient is used alone, and becomes the staple product of prevention and treatment encephalopathy.Preproduction tests more than 3000 name volunteers, it was demonstrated that product of the present invention has satisfied effect to limbs, language, phrenoblabia after the comprehensive health-care of nervous system, especially prevention and reverse headstroke.

Description

A kind of nervonic acid composition and its application in neuroprotection

Technical field

The invention belongs to field of medicaments, and in particular to a kind of nervonic acid composition and its application in neuroprotection.

Background technique

The nervous system disease and cardiovascular disease are to threaten the two major classes disease of human life and health, two class disease incidences All there is close relevance in reason and therapy rehabilitation process.Heart infarction, cerebral infarction stage of attack, generate circulatory disorders, in oxygen and energy Disconnected or insufficient, metabolic disorder destroys permeability of cell membranes, and inside and outside ion transport imbalance causes the damage of nerve cell Wound or apoptosis.The missing of neurogen, but be cause heart infarction, Patients with Stroke limbs, language, mental function disorder it is direct Reason.And disability rate is up to 70%.Considerable distress and heavy financial burden are brought to personal, family.

Although hospital pays much attention to the early rehabilitation training after headstroke, clinically using neuroprotective agent as core Several drugs have positive effect a bit, but generally speaking, at present to the side such as patient's limbs, language, mind after heart infarction, cerebral infarction There are no highly effective methods for the dysfunction in face, especially safely and effectively natural drug or health care product, not yet appear in the newspapers Road.

Summary of the invention

In view of this, the application the purpose of the present invention is to provide a kind of nervonic acid composition and its in neuroprotection, There is significant control efficiency to limbs, language, the mental function disorder after common encephalopathy, headstroke.

In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:

The present invention provides a kind of nervonic acid composition, every 0.5g nervonic acid composition is comprised the following components in parts by weight:

5~100mg of nervonic acid, 12~180mg of phosphatidylserine, 3~300mg of DHA algal oil, celery oil 20~ 300mg, 10~120mg of ginseng extract, 10~180mg of siberian Ginseng P.E, 5~100mg of ginkgo biloba p.e, taurine 2 ~300mg, 5~250mg of vitamin C, 1~100mg of yeast zinc, 0.1~50 μ g of biotin, 0.1~10mg of vitamin B1, 3~200mg of 1~10mg of vitamin B6 and L-Trp；

The nervonic acid is cis-15-tetracosenoic acid；

DHA content is met the following requirements in the DHA algal oil: 10%≤DHA mass percentage≤60%.

Preferably, every 0.5g nervonic acid composition comprises the following components in parts by weight:

15~50mg of nervonic acid, 18~60mg of phosphatidylserine, 5~100mg of DHA algal oil, celery oil 30~ 70mg, 18~80mg of ginseng extract, 18~80mg of siberian Ginseng P.E, 5~30mg of ginkgo biloba p.e, taurine 2~ 50mg, 10~150mg of vitamin C, 3~10mg of yeast zinc, 0.5~5 μ g of biotin, vitamin B11~6mg, vitamin B6 5~50mg of 2~8mg and L-Trp.

Preferably, the ginseng extract extracting method the following steps are included:

1) it by the 80% ethanol water refluxing extraction of volumetric concentration of 5 times of quality of Ginseng Root Powder 3~4 times, extracts every time Time is 2h, is separated by solid-liquid separation, and liquid phase is merged, and heating removal ethyl alcohol obtains extracting solution；

2) when the extracting solution being concentrated into 1.5 times for being equivalent to Ginseng Root Powder quality, add 2 times of Ginseng Root Powder quality Distilled water mixing, then be added into obtained mixed liquor with ether defatting 1~2 time of distilled water equivalent volumes, stratification, By obtained water layer with water layer equivalent water saturation extracting n-butyl alcohol 6~7 times, merge n-butanol phase, water bath method obtains ginseng Total saponin crude product；

3) the ginsenoside crude product is dissolved with methanol, the acetone precipitation of 2~6 times of volumes of again with methanol will be collected Sediment 70 DEG C be dried in vacuo, obtain the ginseng extract of mass concentration containing panaxoside 2.1%.

Preferably, the siberian Ginseng P.E extracting method the following steps are included:

A. by 75% ethanol water of volumetric concentration of 6 times of quality of Manyprickle Acanthopanax Root at 80 DEG C heating and refluxing extraction 2 It is secondary, 6h is extracted every time, is separated by solid-liquid separation, liquid phase is merged, and heating removal ethyl alcohol obtains extracting solution；

B. the extracting solution is concentrated under reduced pressure, dry, the wilsonii for obtaining mass content containing senticosus saponins 2.5% mentions Take object.

Preferably, the ginkgo biloba p.e extracting method the following steps are included:

1. the ethanol water that Ginkgo Leaf and volumetric concentration are 70% is mixed and impregnates 10~12h, the leaching that will be obtained Bubble liquid, to be ultrasonically treated 25min under conditions of 100W, obtains ultrasonic material in 25 DEG C, power；

2. the ultrasonic material is cleaned, extracting solution is obtained；

3. be 1:1.2 by the extracting solution distillation and concentration to relative density, flavones crude extract is obtained；

4. the flavones crude extract is dry, obtain the ginkgo biloba p.e of general flavone mass content 24%.

Preferably, the nervonic acid is vegetative nerve acid, and the raw material of the vegetative nerve acid includes edible containing nervonic acid Vegetable oil and its manufactured powdered oil or the crystal nervonic acid separated from the food plant oil extract containing nervonic acid.

Preferably, the purity of the phosphatidylserine is 50.0%~60.0%.

Preferably, benzofuranone substance is contained in the celery oil；The benzofuranone substance accounts for celery 58.3% or more of seed oil quality, 3- normal-butyl -4,5- Dihydrobenzofuranes ketone in the benzofuranone substance account for celery The 24.29% of vegetable seed oil quality.

The present invention provides the nervonic acid compositions to apply in the drug in terms of preparing neuroprotection.

Preferably, the neuroprotection includes the neuroprotection in terms of establishing Doppler flow mapping after headstroke, is reduced or avoided Hemiplegia, aphasia, peripheral neuropathy side caused by patient limb, the dysfunction of language and spirit and deformity aspect, headstroke Face promotes intellectual development, delays brain aging, repairing neural cell injury and/or prevention improvement encephalopathy aspect.

The present invention provides a kind of nervonic acid compositions, use Chinese medicine monarch composition principle, complete technical side Case design: nervonic acid and phosphatidylserine are monarch drug in a prescription, and the two is neuron membrane, glycosyl sphingolipid, sphingomyelins are main in myelin Ingredient, use in conjunction can completely promote the growth and development of neurocyte, dendron, aixs cylinder, establish extensive synaptic contact, The raising of the promotion brain function of specificity；Realize that function and the reparation dredging nerve information transmission path of adjustment cell membrane are two-tube Under neat, the therapy mechanism of double gain；DHA algal oil and celery oil are ministerial drug, and ministerial drug is the blood flow for improving brain, is refreshing Energy support is provided through member.The function of brain maintains and neural restoration under brain cell compromise state, it is necessary to rely on sufficient blood The supply of flow quantity, oxygen and energy.Once these substances interrupt, it just will form that nervous system is serious, even irreversible damage Wound；Celery oil (being rich in Butylphthalide) and DHA combination, provide important composition fatty acid for brain and cerebral cortex, expand The blood supply of brain improves the oxygen content of blood, protects mitochondria, activates microcirculation.It is monarch drug in a prescription so as to realizing neuroprotective efficacy Important element；Ginseng, wilsonii, ginkgo leaf are adjutant, and adjutant assists monarch drug in a prescription, ministerial drug to play expansion blood vessel, anti-oxidant, kidney tonifying Calm the nerves, improves the effect of peripheral circulation.Three taste of adjutant is China's traditional Chinese medicine, and the soaping agents being rich in, flavonoids etc. are abundant Biotic component has extensive pharmaceutical ground；But the present invention tests discovery, the combination of three taste medicines has synergy to neuroprotection Remarkable effect uses the drug action for having 1+1+1 > 3 than simple；Three taste medicines and monarch drug in a prescription, ministerial drug are combined, and it is more to have played Chinese medicine Brain tonic and intelligence development effect of ingredient, multipath, increases or expands monarch drug in a prescription and replenish qi to invigorate the spleen, and tonifies the kidney to relieve mental strain, expands blood vessel, inhibits blood Platelet cohesion, adjusts the functions such as endocrine at anti-inflammatory, anti-aging；Taurine, vitamin c, zinc, biotin, vitamin B1, dimension Raw element B6, L-Trp, and be known as making medicine.These small-molecule active substances maintain the proliferation of brain, differentiation and development and function It is essential nutrients, has indispensable synergistic function to the performance of monarch drug in a prescription function.

Nervonic acid composition provided by the invention is by monarch principle nervonic acid, phosphatidylserine, DHA, celery Ten plurality of raw materials such as seed oil (Butylphthalide), Chinese medical extract, nutrient prime replenisher carry out organic complex, make the nervonic acid Composition summarizes neurotrophic agent, nerve fibre renovation agent, the activator of neurotransmitter, and neural energy ensures that agent is all Multimachine system promotes the release of neurotransmitter, accelerans arteries and veins so that multisystem, multipath improve the permeability of neuron membrane Dredging nerve information channel, activation or the function for reversing brain are repaired in the conduction of punching.Nervonic acid composition provided by the invention It can promote the metabolism of the heart, brain tissue, participate in growth, differentiation and the regenerative process of brain tissue neuron, improve brain blood With brain metabolic function, dysfunction caused by cardiac muscle and brain diseases can be prevented and treated.Nervonic acid composition provided by the invention is broken through The limitation and deficiency of a certain ingredient is used alone, becomes the staple product of prevention and treatment encephalopathy.

Meanwhile nervonic acid composition provided by the invention is a kind of heart, the synchronous prevention product of cerebrovascular disease, is able to satisfy not With the rigidity demand of age bracket encephalopathy patient, short treating period, significant effect has in the big health field of cardiovascular and cerebrovascular diseases prevention and treatment Substantial advance.Nervonic acid composition can prevent and treat common encephalopathy and change especially to the disability rate after prevention or reduction headstroke Limbs, language, mental function disorder caused by kind and reverse headstroke, are that existing drug and health care product are all inaccessiable, difficult With the effect of confidence.It can be reduced the pain of several ten million patient with cerebral apoplexies every year, there is great social value, there is novelty. Nervonic acid composition primary raw material provided by the invention derives from natural plants, especially contains rare plant, uses most Advanced most practical extraction and separation process technology, biotic component is not only more safer than existing chemicals, but also than chemistry Drug is more effective, and the completely new natural medicine and health care product of this prevention and treatment encephalopathy has novelty.What the present invention used contains mind Vegetable seeds through acid, celery seed, algae etc. is the regenerated resources not being fully developed at present, and invention product pushes away Extensively, the comprehensive utilization of these agricultural resources will be promoted, it is good in economic efficiency, it is practical.Nervonic acid composition provided by the invention Preparation manipulation is strong, and market prospects are good, and product is suitable for scale industrial production.

Specific embodiment

Nervonic acid (nervonic acid, NA) 5~100mg, phosphatidylserine (phosphatidylserine, PS) 12~180mg, DHA algal oil (DHAAlgal Oil) 3~300mg, celery oil (Celery seed oil) 20~300mg, people 10~120mg of conopsea extraction, 10~180mg of siberian Ginseng P.E, 5~100mg of ginkgo biloba p.e, 2~300mg of taurine, 5~250mg of vitamin C, 1~100mg of yeast zinc, 0.1~50 μ g of biotin, 0.1~10mg of vitamin B1, vitamin B6 3~200mg of 1~10mg and L-Trp；

The nervonic acid is suitable -15- tetracosenoic acid；

Nervonic acid composition provided by the invention includes nervonic acid.In every 0.5g nervonic acid composition, the nervonic acid is 5~100mg, preferably 15~50mg, most preferably 18mg.The nervonic acid includes vegetative nerve acid；The vegetative nerve acid Raw material include edible vegetable oil containing nervonic acid and its manufactured powdered oil or from the food plant oil extract containing nervonic acid Isolated crystal nervonic acid；The edible vegetable oil containing nervonic acid is preferably acer truncatum seed oil.The source raw material of nervonic acid and Neural acid content in manufactured goods, can be by the method Accurate Determining of GB5009.168.Nervonic acid is cis-15-tetracosenoic acid, It is a kind of speciality chain monounsaturated fatty acids, is the mark of the core component of cranial nerve fiber and nerve cell, brain glucoside medullary substance Property ingredient.And nerve cell especially brain cell, optic cell, peripheral nerve growth, develop again and function maintain must The nutrient needed, is mainly present in human body brain tissue in the form of glycosyl sphingolipid and sphingomyelins, is risen in the biosynthesis of myelin Important function.Nervonic acid completes information transmitting by ion channel on neuron membrane and receptor and Information procession plays Important adjustment effect；Studies have shown that nervonic acid has protective effect to brain tissue, cardiovascular and cerebrovascular, it is that scientists from all over the world generally acknowledge First and only one can repair dredging injured brain nerve pathway-nerve fibre in the world, and promote nerve cell Regenerated economic benefits and social benefits mystery substance.Higher blood plasma nervonic acid level can reduce the occurrence risk of acute ischemic cerebral apoplexy, The shortage of nervonic acid will cause brain function decline, brain disorder, cerebral apoplexy sequela, senile dementia, brain paralysis, encephalatrophy, The encephalopathies such as failure of memory, insomnia forgetfulness.Nervonic acid is hardly produced in human body itself, needs to supplement by absorbing in vitro.

Nervonic acid composition provided by the invention includes phosphatidylserine, and the purity of the phosphatidylserine is preferred >=50%.In every 0.5g nervonic acid composition, the phosphatidylserine be 12~180mg, preferably 18~60mg, most Preferably 50mg.The phosphatidylserine, molecular weight are 750~850, can voluntarily be prepared, with soybean lecithin and L- Serine is raw material, and after phosphatide enzymatic conversion reaction, purifying concentration is packed after dry and is made using secondarily purified, It can be by buying commercial product.The purity of phosphatidylserine should reach 50.0%~60.0%.Its content should reach The Ministry of Public Health announces the quality requirement of No. 15 phosphatidylserine in 2010.Phosphatidylserine is the active matter of cell membrane Matter is particularly present in brain cell.Its function mainly improves nerve cell function, adjusts the conduction of nerve impulse, promotes Brain memory function has lipophilicity, blood-brain barrier can be run through after absorption and enters brain, plays vascular smooth of releiving Myocyte increases the effect of brain blood supply.The third generation " intelligent nutrition element " being known as after cholinergic and DHA, " brain is special One nutrient ".

In the present invention, the nervonic acid and phosphatidylserine are monarch drug in a prescription.It is described in every 0.5g nervonic acid composition Ministerial drug is preferably 68~350mg, more preferably 40~100mg, most preferably 68mg.

Nervonic acid composition provided by the invention includes DHA algal oil, in the DHA algal oil content of DHA it is preferred >= 35%.In every 0.5g nervonic acid composition, the DHA algal oil is 3~300mg, preferably 5~100mg, most preferably 21mg. DHA algal oil main component docosahexaenoic acid (DHA).DHA content >=35g/100g.The DHA algal oil can self manufacture, To split pot algae (Schizochytrium sp.) or my Ken Shi pot algae (Ulkenia amoeboida) or the hidden dinoflagellate of Kou Shi (Crypthecodinium cohnii)) kind be raw material, pass through the techniques such as fermentation, separation, purification produce DHA；It can also be with commercially available Product, but DHA algal oil should all reach the quality requirement that the Ministry of Public Health announces No. 3 DHA algal oil in 2010.DHA is the brain hair of people One of the important substance educate, grown up.Account for about 97% of -3 fatty acid of omega in brain, enhancing memory and thinking ability mention The effects of high intelligence, is significant.Facilitate neurotransmission in brain.DHA accounts for 60% of total fatty acids in retina, can increase view Membranochromic pigments molecule improves visual acuity.Therefore most important to intelligence and visual acuity.It is micro- that DHA algal oil extracts from ocean Algae, the transmitting without food chain, use are safer.

Nervonic acid composition provided by the invention includes celery oil.In every 0.5g nervonic acid composition, the celery seed Oil is 20~300mg, preferably 30~70mg, most preferably 60mg.The celery oil includes benzofuranone substance； The benzofuranone substance accounts for 58.3% or more, 3- normal-butyl -4,5- Dihydrobenzofuranes ketone of celery oil quality Mass concentration is 24.29%.The source of the celery oil voluntarily can prepare or buy.The preparation side of the celery oil Method is the dry, crushing, using supercritical CO using celery (Aplum graveolensL) seed as raw material₂Extract celery oil. Optimum process condition are as follows: 60 mesh of raw material granularity, temperature 45 C, pressure 15MPa, flow 15L/h, extraction time 2h.Using super Critical CO₂Not only the high of oil yield maintains the pure natural characteristic of celery oil to extraction celery seed oil, and in oil it is medicinal effectively at Divide content high (being steam distillation method more than five times), the benzofuranone substance with celery bank up the roots of seedlings characteristic perfume abundant, this A little biotic components are the active principles for cerebral arterial thrombosis.The celery seed contains cineol, phosphorus methoxyl group phenol, umbrella flower A variety of biotic components such as lactone, apiolin, linolenic acid, volatility grease, Huang Tong, boron, calcium, iron, sodium, zinc.From celery seed Butylphthalide (apiumgraveolens linn) is extracted, is n butylphthalide levo form.Confirm that it can pass through through clinic Multiple links inhibit blood platelet, thrombosis, reduce infarct size, restore vessel wall elasticity, improve the ischemics such as microcirculation Cardiovascular and cerebrovascular diseases are safe and efficient, take play the role of significant blood pressure lowering in short term, and reducing blood lipid, long-term use is far superior to Other Pharmaceuticals, it is without any side effects.It can inhibit Neuron Apoptosis in During Ischemia again simultaneously, mitigate brain tissue damage The encephalopathies such as degenerative brain disorder, Vascular dementia, diabete peripheral herve pathology, insomnia and depression are also had satisfied treatment by wound Effect.

In the present invention, the DHA algal oil and celery oil are ministerial drug.In every 0.5g nervonic acid composition, the ministerial drug Preferably 81mg.

Nervonic acid composition provided by the invention includes ginseng extract.In every 0.5g nervonic acid composition, the ginseng Extract is 10~120mg, preferably 18~80mg, most preferably 18mg.The raw material Panax slender acanthopanax of the ginseng extract Section plant, earliest record see Shennong's Herbal, have effects that health and cure the disease: " main tonifying five zang organs, soothe the nerves determine soul Soul, stop palpitate with fear, improving eyesight, happy intelligence development, long term usage are made light of one's life by commiting suicide macrobiosis." long-term medical practice confirm ginseng be tonic first of Status.Ginseng especially plays a leading role to anti-aging to the aging of nervous system.

In the present invention, the extracting method of the ginseng extract preferably includes following steps:

Nervonic acid composition provided by the invention includes siberian Ginseng P.E.In every 0.5g nervonic acid composition, the thorn Slender acanthopanax extract is 10~180mg, preferably 18~80mg, most preferably 18mg.The raw material thorn five of the siberian Ginseng P.E It is added in traditional Chinese medicine using having long history, has the function of " bowl spares strengthening the essence, hard muscles and bones, Qiang Zhiyi ", long term usage " making light of one's life by commiting suicide resistance to old " " into diet, strong strength, can not also forget things " with other medicine compatibilities, the effect of " positive people makes yin, and negative people makes sun ". Wilsonii and ginseng have similar pharmacological action and clinical efficacy.

In this application, the extracting method of the siberian Ginseng P.E preferably includes following steps:

A. by 75% ethanol water of volumetric concentration of 6 times of quality of Manyprickle Acanthopanax Root at 80 DEG C heating and refluxing extraction 2 It is secondary, 6h is extracted every time, is separated by solid-liquid separation, water phase is merged, and heating removal ethyl alcohol obtains extracting solution；

Nervonic acid composition provided by the invention includes ginkgo biloba p.e.In every 0.5g nervonic acid composition, the silver Apricot leaf extract is 5~100mg, preferably 5~30mg, most preferably 8mg.The ginkgo biloba p.e main component flavones Glycosides, ginkgolides and monomer ginkgolides etc. are right to cardiovascular system, Brain circlulation, blood and nerve etc. mechanism of action and curative effect Brain tissue and neurotrosis have protective effect.Improve neurogen loss and neurotransmission is obstructed, intelligence decline and Parkinson's disease Symptom.

In the present invention, the extracting method of the ginkgo biloba p.e preferably includes following steps:

1. the ethanol water that Ginkgo Leaf and volumetric concentration are 70% is mixed and impregnates 10~12h, the leaching that will be obtained Bubble liquid is ultrasonically treated 25min under conditions of 25 DEG C, power 100W, obtains ultrasonic material；

2. the ultrasonic material is cleaned, extracting solution is obtained；

In the present invention, the ginseng, wilsonii, ginkgo leaf are adjutant.In every 0.5g nervonic acid composition, the assistant Medicine is preferably 44mg.

Nervonic acid composition provided by the invention includes taurine.In every 0.5g nervonic acid composition, the taurine is 2~300mg, preferably 2~50mg, most preferably 2mg.The taurine is the most abundant free amine group of central nervous system Acid is present in cerebral cortex, the regions such as cerebellum, olfactory bulb.It can promote proliferation, differentiation and the development of cranial nerve cell, enhancing brain is thin DNA intracellular RNA synthesis and utilization to protein.The adjusting for participating in learning and memory influences the acquisition of learning and memory, consolidates With each stage of reproduction.The present invention is not particularly limited the source of the taurine, using ox sulphur known in the art Acid.

Nervonic acid composition provided by the invention includes vitamin C.In every 0.5g nervonic acid composition, the vitamin C For 5~250mg, preferably 10~150mg, most preferably 10mg.The vitamin C is a kind of water soluble vitamin, is distributed to body It is powerful antioxidant in interior all water-soluble structures, the synthesis of ossein can be promoted, promotes the generation of tyrosine and tryptophan It thanks, extends the human body service life.Improve the metabolism of cholesterol, prevent cardiovascular disease, enhances body immunity.The present invention is to the dimension The source of raw element C is not particularly limited, using vitamin C known in the art.

Nervonic acid composition provided by the invention includes yeast zinc.In every 0.5g nervonic acid composition, the yeast zinc is 1~100mg, preferably 3~10mg, most preferably 4mg.The yeast zinc is that human metabolism and growth and development are necessary One of microelement is the important composition ingredient of brain, the content of zinc highest in akrencephalon and cerebral cortex grey matter in brain.Zinc with The differentiation of neurolemma is related, and zinc deficiency easily causes deformity in brain growth early stage；Brain growth late lesion neuron growth and Cynapse occurs, and causes the sequelae of behavior, can influence to recognize.The present invention does not have special limit to the source of the yeast zinc System, using yeast zinc known in the art.

Nervonic acid composition provided by the invention includes biotin.In every 0.5g nervonic acid composition, the biotin is 0.1~50 μ g, preferably 0.5~5 μ g, most preferably 3 μ g.The biotin is also known as biotin, is that fat and protein are normal It is metabolized indispensable substance, is a kind of maintenance human body natural growth, development and the necessary nutrition of normal human's functional health Element.Lack biotin to easily lead to melancholy, insomnia, be easy the nervous symptoms such as doze.The present invention does not have the source of the biotin Have it is specifically limited, using biotin known in the art.

Nervonic acid composition provided by the invention includes vitamin B1.In every 0.5g nervonic acid composition, the vitamin B1 is 0.1~10mg, preferably 1~6mg, most preferably 2mg.Vitamin B1, that is, the thiamine, in body carbohydrate generation It plays an important role in thanking, if lacking thiamine, metabolic process will slack-off or complete interruption.Thiamine is to nervous system The transmitting of high-frequency impulse has an impact.Be deficient in vitamin B1, easily occur brain symptom, ophthalmoplegia, nystagmus, mind it is indifferent, Stupor and convulsions.The present invention is not particularly limited the source of the vitamin B1, using vitamin known in the art B1.

Nervonic acid composition provided by the invention includes vitamin B6.In every 0.5g nervonic acid composition, the vitamin B6 is 1~10mg, preferably 2~8mg, most preferably 2mg.The vitamin B6 can rapidly transform into phosphopyridoxal pyridoxal phosphate in vivo, Play coenzyme in many reactions.Vitamin B6 deficiency can cause the metabolic disorder of amino acid and protein, clinical manifestation For undergrowth, convulsions, anaemia, antibody reduction, skin lesion etc..Vitamin B6 is insufficient, nervous centralis hypoevolutism, myelin It is formed insufficient.The present invention is not particularly limited the source of the vitamin B6, using vitamin B6 known in the art ?.

Nervonic acid composition provided by the invention includes L-Trp.In every 0.5g nervonic acid composition, the L- color ammonia Acid is 3~200mg, preferably 5~50mg, most preferably 5mg.The L-Trp is auxin biosynthesis weight in plant The precursor substance wanted, is a kind of amino acid needed by human, has remarkable effect to memory is improved.The present invention is to the L- color ammonia The source of acid is not particularly limited, using L-Trp known in the art.

In the present invention, the taurine, vitamin c, zinc, biotin, vitamin B1, vitamin B6 and L-Trp To make medicine.It is described to make medicine 25.003mg in every 0.5g nervonic acid composition.

The preparation method of nervonic acid composition provided by the invention, is not particularly limited, and the component is mixed.

In the present invention, the neuroprotection preferably includes to establish after headstroke the neuroprotection in terms of Doppler flow mapping, subtracts Less or avoid hemiplegia caused by the dysfunction and disabled aspect, headstroke of patient limb, language and spirit, aphasia, surrounding refreshing Through in terms of lesion, in terms of promoting intellectual development, delaying brain aging, repair neural cell injury and/or prevention improvement encephalopathy.

In the present invention, the drug further includes auxiliary material.The auxiliary material include vegetable fat powder, sucrose, maltitol, starch, Dextrin, maltodextrin, gelatin, glycerol, water, starch, silica, magnesium stearate, edible vegetable oil, citric acid and/or fruit Essence.According to the needs of production pharmaceutical formulation, can add one such or several.The auxiliary material accounts for the quality percentage of finished product Number is 5%~35%.In the preparation for needing to add auxiliary material, auxiliary material account for finished product mass percent can for 5%, 8%, 10%, 15%, 18%, 20%, 25%, 30%, 35%.

In the present invention, when the dosage form of affiliated drug is capsule or gel candy, the auxiliary material is including but not limited to bright Glue, glycerol, water, it is one or more of in beeswax, titanium dioxide, sugar or sugar alcohol.The production side of the medicament capsule or gel candy Method preferably includes following steps: the grease in nervonic acid composition being mixed, is made mixing in dusty raw materials addition grease Core material；Will be gel solubilizing, packaging material is made in glycerol adding, water.Soft capsule or gel candy are processed by pharmacopeia soft capsule technique.

In addition, it is solid, pharmaceutically acceptable various dosage forms that drug of the present invention, which may be made as content, it is such as common Granule, solid beverage, tablets and other formulations.The product for making solid, needs that edible oil and fat are first fabricated to powdered oil, Then it is mixed with other supplementary materials, then various dosage forms is made.

In the present invention, when the dosage form of the drug is granule, solid beverage or tablets and other formulations, the accessory package Include but be not limited to sugar or sugar alcohol, it is vegetable fat powder, dextrin, neotame, fruit essence, one or more of in magnesium stearate.

The production method of the granule, solid beverage or tablet preferably includes following steps: will be in nervonic acid composition Grease 1.5 times of modified starches and water is added, clipped, homogeneous, drying is made powdered oil, mixes, press with dusty raw materials Pharmacopeia solid pharmaceutical preparation technique is processed into granule, solid beverage, tablets and other formulations.

Below with reference to application of the embodiment to a kind of nervonic acid composition provided by the invention and its in neuroprotection into Row detailed description, but they cannot be interpreted as limiting the scope of the present invention.

Embodiment 1

Plant oil extract containing nervonic acid

It by the impurity elimination of acer truncatum seed, peels off, selection by winnowing obtains Acer Truncatum Buge kernel, and it is 300 that Acer Truncatum Buge kernel, which is ground into granularity, Micron fine powder below；Using petroleum ether as Extraction solvent, ultrasonic extraction is carried out；The feed liquid mass ratio of petroleum ether and kernel powder is 1 : 10, ultrasonic power 50W, ultrasonic time 70min, temperature are 38~42 DEG C.The refined peroxide value that obtains of crude oil is lower than 0.20g/100g grease.

Embodiment 2

The production of powdered oil

Vegetable oil containing nervonic acid or 1 part of celery oil, 1.5 parts of starch Sodium Octenyl Succinate, 1.5 times of sodium starch pure Change water.Starch Sodium Octenyl Succinate purified water is first impregnated into 6h, nervonic acid vegetable oil or celery seed is added in stirring and dissolving Oil, material (12000 turns/min) shearing 10min of cutter, material import homogenizer, control 40~60 DEG C of temperature, pressure 10 ~18MPa, material homogeneous 2 times, will be homogeneous after material be spray-dried, 15~20MPa of atomisation pressure, inlet temperature 140 It is~160 DEG C, 70~80 DEG C of temperature of outgoing air, spray-dried that powdered grease is made.

Embodiment 3

The separation of crystal nervonic acid powder

Nervonic acid extracts separation from the vegetable oil such as acer truncatum, malania oleifera and obtains, and specifically comprises the following steps:

(1) coarse extraction: the 2-5 times of ethyl alcohol for measuring 70-100% is added into Malania Oleifera Oil, homogeneous solution is stirred into, to institute State 20~40% sodium hydroxide or potassium hydroxide solution that 1~3 times of Malania Oleifera Oil quality amount is added in solution, heating stirring Complete to saponification, filtration, acidification of filtrate to pH value 3~4 is stood, and the solidification of collection upper layer is saponified, is washed to neutrality, is dripped Moisture to the greatest extent, obtains neural acid crude；

(2) it crystallizes: taking the neural acid crude, carry out following crystallization operation:

The volume ratio of dehydrated alcohols and acetone that 3.8~4.2 times of amounts are added into the neural acid crude be 1: 1 it is mixed Solution is closed, 60~70 DEG C are heated to reflux and make to dissolve, and filter, and collect filtrate and simultaneously stand in -5~-10 DEG C, and crystallization is collected in centrifugation； The crystallization operation obtains nervonic acid primary crystal；

(3) it recrystallizes: repeating crystallization operation 2~7 times in step (2), until neural acid monomers in gained crystallization Content not less than 85% to get.

Embodiment 4

Celery oil extracts

Celery oil is the dry, crushing using celery (Aplum graveolensL) seed as raw material, and use is overcritical CO₂Extract celery oil.Specific extracting factor are as follows: 60 mesh of raw material granularity, temperature 45 C, pressure 15MPa, flow 15L/ H, extraction time 2h.Supercritical CO₂Not only oil yield is high for the celery seed oil of extraction, also maintains the pure natural characteristic of celery oil. And medicinal active constituent content is high (being steam distillation method more than five times) in oil.Benzo furan with celery bank up the roots of seedlings characteristic perfume Letones of muttering account for about 58.3%, and wherein 3- normal-butyl -4,5- Dihydrobenzofuranes ketone is 24.29%.These biotic components are Active principle for cerebral arterial thrombosis.

Embodiment 5

The preparation of nervonic acid composition soft capsule

It is prepared by acer truncatum seed oil 30kg, DHA algal oil 2.1ke, phosphatidylserine 5kg prepared by embodiment 1, embodiment 4 Celery oil 6kg, ginseng extract 1.8kg, siberian Ginseng P.E 1.8kg, ginkgo biloba p.e 0.8kg, taurine 0.2kg, vitamin C 1kg, yeast zinc 0.4kg, biotin 0.3 μ g, B1 0.2kg, B6 0.2kg, L-Trp 0.5kg.

By above-mentioned formula weighing material make core material, take gelatin, glycerol, water production capsule material, through pelleting, wash ball, select ball, Cool ball, polishing are examined, packaging, storage.Soft capsule 100,000 containing nervonic acid, every 0.5g is made.

Embodiment 6

The preparation of nervonic acid composition gel candy

Garlic fruit seed oil 25kg, DHA algal oil 4kg, phosphatidylserine 4kg prepared by embodiment 3, prepared by embodiment 4 Celery oil 10kg, ginseng extract 2kg, siberian Ginseng P.E 1.2kg, ginkgo biloba p.e 1.5kg, taurine 0.8kg, Vitamin C 1kg, yeast zinc 0.6kg, biotin 0.8 μ g, B1 0.1kg, B6 0.4kg, L-Trp 1kg.

By above-mentioned formula weighing material, glucose syrup is separately added, dissolution is stirred off, and core material is made；Take gelatin, glycerol, water system Make coating material, coating material is sprayed at sugared wicking surface, cooling and shaping；Enzyme deactivation is examined, packaging, storage, is made containing nervonic acid 100,000 pieces of gel candy of coating.

Embodiment 7

The preparation of nervonic acid composition particle

Acer truncatum powdered oil 9kg, DHA powdered oil 0.45kg prepared by embodiment 1, celery seed prepared by embodiment 2 Oil powder powder oil 1.28kg, phosphatidylserine (50%) 0.5kg, ginseng 0.18g, wilsonii 0.16g, ginkgo leaf 0.08kg, Taurine 0.02kg, vitamin c 0.1g, yeast zinc 0.04kg, biotin 0.03mg, B1 0.02kg, B6 0.02g, L- color ammonia Sour 0.05kg, vegetable fat powder 9kg and maltitol 9.08.

By above-mentioned formula weighing material, maltodextrin is separately weighed, the auxiliary materials such as sweetener, flavouring agent, through mixing, granulation, whole Grain, dry, granule packaging, are examined, packaging, storage.10,000 bags of nervonic acid composition granule, every bag of 3g is made.

Embodiment 8

The preparation of crystal nervonic acid composition tablet

Crystal nervonic acid powder 180g prepared by embodiment 2, DHA algal oil powdered oil 450g, celery prepared by embodiment 2 Rapeseed oil powdered oil 1280g, phosphatidylserine (PS≤60%) 500g, ginseng extract 180g, siberian Ginseng P.E 180g, ginkgo biloba p.e 80g, taurine 20g, vitamin c 100g, yeast zinc 40g, biotin 30 μ g, B1 20g, B6 20g, L-Trp 50g.

By above-mentioned formula weighing material, separately weigh starch 1900g, through mixing, granulation, whole grain, drying, tabletting, inspection, Packaging, storage.10,000, crystal nervonic acid composition tablet, every 0.5g is made.

Embodiment 9

The acer truncatum seed oil 300mg (being equivalent to crystal nervonic acid 18mg) of 6% neural acid content, DHA algae oil (DHA≤ 40%) 21mg, phosphatidylserine (PS≤60%) 50mg, celery oil 60mg, ginseng extract 18mg, wilsonii extract Object 18mg, ginkgo biloba p.e 8mg, taurine 2mg, vitamin c 10mg, yeast zinc 4mg, biotin 3 μ g, B1 2mg, B6 2mg, L-Trp 5mg.

Pharmaceutical adjunct: including but is not limited to gelatin, glycerol, water, one kind or several in beeswax, titanium dioxide, sugar or sugar alcohol Kind.

Manufacture craft: the grease in formula is mixed, and mixing in grease is added in dusty raw materials, core material is made；It will be bright Peptized, packaging material is made in glycerol adding, water.Soft capsule is processed by pharmacopeia soft capsule technique.

Embodiment 10

2% acer truncatum powdered oil 900mg (being equivalent to pure nervonic acid 18mg), DHA algal oil powdered oil 45mg are (quite In DHA algal oil 21mg), celery oil powdered oil 128mg (being equivalent to celery oil 60mg), phosphatidylserine (PS≤ 60%) 50mg, ginseng extract 18mg, siberian Ginseng P.E 18mg, ginkgo biloba p.e 8mg, taurine 2mg, vitamin C10mg, yeast zinc 4mg, biotin 3 μ g, B1 2mg, B6 2mg, L-Trp 5mg.It is made 3g/ bags.

Pharmaceutical adjunct: including but is not limited to sugar or sugar alcohol, vegetable fat powder, dextrin, neotame, fruit essence, one in magnesium stearate Kind is several.

Manufacture craft: 1.5 times of modified starches and water are added in the grease in formula, powder is made in clipped, homogeneous, drying Powder oil is mixed with dusty raw materials, is processed into granule dosage form by pharmacopeia solid pharmaceutical preparation technique.

Embodiment 11

Nervonic acid composition effect experiment of the present invention

Pharmacodynamic test of the nervonic acid composition of the present invention to brain dementia and cognition dysfunction

1, tested case:

Tested patient 80 for meeting diagnostic criteria are chosen, treatment group and control group are randomly divided into.Wherein treatment group 40 Example, male 22, female 18；It is age 59~78 years old, 73 years old average；It is the course of disease 6 months~6 years, 3.2 years average；Education level 9.85 ± 6.73 years.Control group 40, male 29, female 11；It is age 60-81 years old, 75 years old average；The course of disease 7 months~8 years, It is 5.23 average；Education level 9.20 ± 5.5 years.Two groups gender, the age, the course of disease, in terms of compare Difference is not statistically significant (P > 0.05), is comparable.

2, diagnostic criteria

Referring to the international Disease Diagnosis Standard of senile dementia, it is dull-witted to determine that the detection of diagnostic criteria (1) intelligence confirms.(2) At least 2 identification function obstacles.(3) memory and cognitive disorders progressive aggravate.(4) unconscious obstacle；(5) 40-90 years old Onset.(6) without body associated with the above-mentioned state of an illness or brain diseases.And it excludes brain trauma, brain tumor, insane, extremely weigh Spend dement.Disturbance of intelligence situation is by Chang Guchuanshi dementia modification scale (HDS) and Mini-mental Status Examination evaluation criteria (MMSE).The diagnosable dementia of total score < 16 point HDS person, MMSE is different according to education degree, and it is more than middle school≤24 points, junior middle school≤21 Point, illiteracy≤l7 points.There are obvious obstacle in total score > 22 point daily living measuring scale (ADL) for function.Observation index: observation Pretherapy and post-treatment blood, urine, feces routine and hepatic and renal function situation of change.

3, treatment method

Treatment group takes orally nervonic acid composition (embodiment 5,0.5g/) of the present invention, 2 times a day, 3 tablets each time.Control group Oral nicergoline tablets (5mg/ piece) 3 times a day, 2 tablets once.3 months are 1 course for the treatment of, observe curative effect after the course for the treatment of.

4, efficacy assessment standard

The criterion of therapeutical effect revised on the national senile dementia special topic Symposium Held held referring to the nineteen ninety whole nation association of traditional Chinese medicine:

Effective: cardinal symptom disappears substantially, conscious, and orientation is sound, answers a question and is in the main true, and reaction is more clever It is quick, it takes care of oneself, can be carried out general social activities.It compares before and after treatment, HDS, MMSE score improve 25% or more, and ADL is obtained Dividing reduces by 25% or more.

Effective: main spirits symptom is weakened or partial disappearance, takes care of oneself, and answers a question and is in the main true, but reacts Blunt, intelligence and personality still have partial impairment.10% or more is improved compared to HDS, MMSE score before and after treatment, ADL score reduces 10% or more.

Invalid: main clinic symptoms still have development without change or the state of an illness, can't take care of oneself, answer a question incorrect, mind Will is dull-witted.It compares before and after treatment, HDS, MMSE score improve 10% or less or without significant changes, and ADL score reduces by 10% or less Or without significant change.

5, experimental result and analysis

5.1, two groups of curative effects compare: treatment group's total effective rate is 90.00%, and control group 70.00%, two groups are compared, P > 0.05.It is shown in Table 1.

The comparison of 1 two groups of curative effects of table

Group	Total number of cases	It is effective	Effectively	In vain	Always use efficiency (%)
						Treatment group	40	10	26	4	36(90.00)
Control group	40	5	23	12	28(70.00)

5.2, two groups of scale integral contrasts: treatment group HDS, MMSE, ADL integral are compared with before treatment, P < 0.05；With it is right Compare according to group, P < 0.01.It is shown in Table 2.

2 two groups of scale integral contrasts (dividing) of table

Group	Number of cases	HDS	MMSE	ADL
					Treatment group	40
Before treatment		12.82±5.38	15.45±2.10	59.12±6.62
					After treatment		19.85±6.74	19.71±2.62	54.55±7.43
Difference		5.91±4.34	3.86±2.23	4.70±2.34
					Control group	40
Treatment group		13.81±5.33	15.96±2.44	60.20±6.73
					Before treatment		15.30±5.28	18.98±3.03	58.12±6.81
After treatment		1.78±1.22	2.29±2.65	2.18±2.85

P < 0.05 * compared with before treatment, compared with the control group, P < 0.01 △.

Compared with control group is by Germicidal efficacy, total effective rate otherness is smaller (P > 0.05) for treatment group, in scale product On, there is apparent otherness (P < 0.01) between 2 groups, show that nervonic acid composition of the present invention is improving brain dementia patient's Control group is substantially better than on disturbance of intelligence, raising cognitive function and activity of daily living.

Experimental example 12

Nervonic acid composition of the present invention is to the mind formed after headstroke and apoplexy, the pharmacodynamic test of language, physical handicaps

1, tested case

According to Chinese Medical Association's Second National cerebrovascular disease academic conference revision " all kinds of cerebrovascular disease diagnosis are wanted Point ", selection meets patient 245 of diagnostic criteria, is randomly divided into treatment group and control group, makes gender, age, state of an illness weight Degree, course of disease length are comparable.Wherein treatment group 160, male 118, female 42, the age 46~58 years old；The course of disease 6 days ~5 years；This group Internal Carotid System cerebral thrombosis 124, Vertebro-basilar System cerebral thrombosis 36；Hemiptegic 140 in 160 Example, wherein completeness hemiplegia 51, imperfection hemiplegia 89, aphasia 126, brain dementia 6, complicated hypertension 81, Coronary heart disease 49.

Control group 85, male 59, female 26, the age 45~75 years old；The course of disease 10 days~7 years；Internal Carotid System brain blood Hemiplegia 74 in 66, bolt, Vertebro-basilar System 19,85, wherein completeness hemiplegia 28, imperfection hemiplegia 46 Example, aphasia 58, complicated hypertension 55, coronary disease 38 examples.

2, treatment method

Treatment group takes orally nervonic acid composition (embodiment 5,0.5g/) of the present invention, 2 times a day, 3 tablets each time, control group Oral xiaoshuan tongluo tablet (main component Rhizoma Chuanxiong, Radix Salviae Miltiorrhizae, Radix Astragali, rhizoma alismatis, Radix Notoginseng, sophora flower, ramulus cinnamomi, Radix Curcumae, radix aucklandiae, borneol, mountain Short, bristly hair or beard, campanulaceae, every slice weight 0.38g), 3 times a day, 6 tablets once.Other anti-platelet aggregation medicinals and blood vessel are deactivated during treatment Dilator.Observing time: 30 days are 1 course for the treatment of, and 3 courses for the treatment of terminate to evaluate curative effect.Observed content: the mind of patient, language and Motor function；Nervous system signs；Instrument audit report.Recording method: press apoplexy point system, mainly evaluate mind, The recovery extent of language, motor function.

3, curative effect determinate standard

Full marks 28 divide before treating, and not more than 18 points of starting point point, efficacy evaluation method: [(pre-treatment score-is controlled Integrated after treatment) ÷ pre-treatment score] × 100%, it is expressed as a percentage.

(1) it is almost recovered: >=85%, neurological symptom and sign disappear substantially, live and take care of oneself completely, the state of an illness is restored Degree is up to 85% or more；(2) effective: >=50%, neurological symptom and sign largely disappear, can independent walking, life portion It point takes care of oneself, state of an illness recovery extent is up to 50~85%；(3) effectively: >=20%, sings and symptoms partial disappearance, support can leave the bed station It is vertical, it can't take care of oneself, state of an illness recovery extent is up to 20~50%；(4) invalid: < 20%, the state of an illness is not improved or deterioration person.

4, test results and analysis

4.1, basic efficacy analysis:

It in treatment group 160, fully recovers 59, effective 62, improves 25, total effective rate is up to 91.3%；Control group 85 Example is fully recovered 17, effective 19, is improved 25, and total effective rate is 71.8%. treatment

As a result see Table 3 for details.

The comparison (number of cases, %) of 3 two groups of curative effects of table

Group	Total number of cases	Recovery from illness	It is effective	It improves	In vain	Always use efficiency
							Treatment group	160	59(36.9)	62(38.8)	25(15.6)	14(8.8)	146(91.3)
Control group	85	17(20.0)	19(22.4)	25(29.4)	24(28.2)	61(71.8)

4.2, cardinal symptom changes and analyzes before and after treatment:

Four primary symptom hemiplegia that rear headstroke is formed, facial paralysis, dysphonia and dizziness are treated, is obviously changed Kind, this nervonic acid composition is respectively 67.13%, 72.45%, 71.43%, 83.48% to primary symptom elimination factor, eliminating thrombus and removing obstruction in channels Piece is respectively 45.83%, 63.27%, 57.14%, 73.77%, is hindered with this nervonic acid composition to the human body that headstroke is formed The elimination and improvement hindered is more significant, and as a result see Table 4 for details.

(number of cases, %) is compared in the variation of the pretherapy and post-treatment cardinal symptom of table 4

Experimental example 13

Nervonic acid composition of the present invention is to the effect experiment for improving children's memory quotient

80 ages identical child is selected to carry out randomized double-blind experiment.Experimental group 40 are taken addition nervonic acid of the present invention The food (embodiment 7, daily 3g) of composition, control group takes the food for not adding nervonic acid composition of the present invention, continuous to take With 30 days.By the standard of test memory quotient, 7 contents such as intelligence, understanding, association are tested respectively, then calculate two The average memory quotient (IQ) of group.

Conclusion: taking nervonic acid 30 days, and control group is averaged IQ 98.12 ± 15.55, experimental group be averaged IQ 128.46 ± 16.18 memory quotient is significantly increased.As a result see Table 5 for details.

IQ variation is compared before and after table 5 eats nervonic acid composition

Embodiment 14~21 is that the test-meal effect of nervonic acid composition of the present invention is illustrated.

Product of the present invention has been subjected to the test-meal of more than 3000 people during test manufacture, is mixed with to different types of encephalopathy Efficiency is up to 90% or more.The deep favor by consumer with gasp in admiration, name just a few example:

Embodiment 14

Nervonic acid composition product is taken after heavy cerebral thrombosis in 48 hours, body and mind is avoided and disables

Liu male lives Jilin Province somewhere for 48 years old

There are 10 years hypertension, hyperlipidemia past medical history, happen suddenly large area cerebral thrombosis during Tianjin is gone on business within 2 months 2017, Doctor judged such large area infraction at that time, disables be inevitable after being ill.For the trouble on the basis of conventional therapy, 48 is small When interior beginning with nervonic acid composition product, CT examination after 15 days is taken, cerebral infarction penumbra region collateral of nerve recycles to be formed, and even served 2 Month, body fully recovers, and mind, language, limbs do not leave any obstacle, and the activities such as work, drive now are normal.Liu Certain is said, is gone to promote this good product with the full energies of my latter half of one's life, allow thousands upon thousands heart infarctions, cerebral infarction same patient, exempt from Go the hardship of deformity！

Embodiment 15

9 years patients that are paralyzed in bed after cerebral thrombosis, limb function is significantly restored after taking nervonic acid composition 5 days

River, singer ancestral home Yanbian, Jilin Province city of male 45 years old Beijing

Before 9 years, lower limb hemiplegia is unable to leave the bed after river mother suffers from cerebral thrombosis, and mother is carried on the back onto wheelchair by river on ordinary days, Outdoor activities could be arrived.After taking nervonic acid composition 3 days, lower limb become soft, there is consciousness, can be movable, mother's energy after 5 days Push wheel chair normal walking.Mothers and sons are very excited, and exclaiming this is that the thing that dare not also think of having a dream comes true, and then river is sat It on wheelchair, allows mother to push him with wheelchair and walks, taken the video at this unforgettable moment, share in circle of friends！

Embodiment 16

8 years patients that are paralyzed in bed after cerebral thrombosis take independent ambulation after nervonic acid composition 5 days

Liu 52 years old bank of Heilongjiang Province vice-president of male

It is limited to suffer from lower limb exercise after cerebral thrombosis before 9 years, after primary weight fall events again under not bed, take nerve After acid composition 5 days indoors can independent ambulation, his household's scream: this is too magical, too big this of thanks Shenyang medicine Good product！From the widespread that shoots the video.

Embodiment 17

Suffer from 4 years aphasia (mute) people of encephalopathy, lifts up one's voice again after taking nervonic acid composition

Mr. Wang: male 54 years old, lives the Arong Banner Hu Lun Bei Meng.

Because brain stem neurological disorder leads to aphasia before 2014, slobber.Used many drugs do not improve.He takes It, can toot sending single syllable with 20 days after nervonic acid composition；After 30 days, can say compared with simple statement；It 45 days, speaks It is normal, continue to take, voice is gradually clear normal.

Embodiment 18

Facial paralysis patient after headstroke restores as before after taking nervonic acid composition

It is neat certain: female, 56 years old, associate chief physician.Hospital of Heilongjiang Province

The corners of the mouth is turned left after headstroke, and side surface part is crooked, and glossolalia, patient is very painful, and physical therapy, drug therapy are not got better Turn.After taking nervonic acid composition two weeks, the askew phenomenon of mouth is gradually corrected, and after two months, is returned to normal.Doctor's Qi admiration: It takes up a job as a doctor decades, never meets the product of such mystery, be really that a medicine is hard to find.

Embodiment 19

20 years schizophrenic patients are fully recovered after taking nervonic acid composition

Huang, female 38 years old, live Hulunbeir League Arong Banner

Because spirit is stimulated at 18 years old, depression occurs so that developing to serious schizophrenia, once lights oneself twice House do not improve always over 20 years, bring angor to household！Take nervonic acid composition 2 months, illness is quickly It improves, life can take care of oneself after 3 months, and thinking restores normal, can give a lecture in the conference of hundreds of people.Thank to nervonic acid composition New life is brought to him！

Embodiment 20

11 years patients Parkinson, no longer tremble after taking nervonic acid composition

Mr. Yu male lives Inner Mongol somewhere for 44 years old

Suffer from Parkinson's disease 11 years, hand and trembling of limbs, hand can not press from both sides dish when having a meal, and nickname " is trembled " in greatly.With nerve Acid composition 20 days, hand was no longer shaken, and can hold a pen and write, and even served basic rehabilitation in two months, he say " I had never expected this product this It is overbearing, allow " trembling in greatly " no longer to tremble unexpectedly！

Embodiment 21

Suffer from senile dementia within 8 years, it is normal to take nervonic acid composition reminiscence in two months

Jiang male lives Liaoning Panjin City unit organ retired cadre for 71 years old

Mental state is droning after retirement, and mind depression gradually develops into senile dementia.Classical symptom is by failure of memory, amnesia So that losing memory to serious, it can be the name of son, grandson, go out to can not find oneself door, lean body mass, general weakness is lived It is dynamic limited.With nervonic acid composition 2 months, memory was restored to this age bracket normal level, and glowing with health, action Flexibly.

The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications It should be regarded as protection scope of the present invention.

Claims

1. a kind of nervonic acid composition, which is characterized in that every 0.5g nervonic acid composition comprises the following components in parts by weight: nerve 5~100mg of acid, 12~180mg of phosphatidylserine, 3~300mg of DHA algal oil, 20~300mg of celery oil, ginseng extract 10~120mg, 10~180mg of siberian Ginseng P.E, 5~100mg of ginkgo biloba p.e, 2~300mg of taurine, vitamin C 5 ~250mg, 1~100mg of yeast zinc, 0.1~50 μ g of biotin, 0.1~10mg of vitamin B1,1~10mg of vitamin B6 and 3~200mg of L-Trp；

The nervonic acid is cis-15-tetracosenoic acid；

2. nervonic acid composition according to claim 1, which is characterized in that every 0.5g nervonic acid composition includes following heavy Measure the component of part:

15~50mg of nervonic acid, 18~60mg of phosphatidylserine, 5~100mg of DHA algal oil, 30~70mg of celery oil, ginseng 18~80mg of extract, 18~80mg of siberian Ginseng P.E, 5~30mg of ginkgo biloba p.e, 2~50mg of taurine, vitamin C 10~150mg, 3~10mg of yeast zinc, 0.5~5 μ g of biotin, 1~6mg of vitamin B1, vitamin B6 2~8mg and L- color 5~50mg of propylhomoserin.

3. nervonic acid composition according to claim 1 or 2, which is characterized in that the extracting method of the ginseng extract The following steps are included:

1) by the 80% ethanol water refluxing extraction of volumetric concentration of 5 times of quality of Ginseng Root Powder 3~4 times, each extraction time It for 2h, is separated by solid-liquid separation, merges liquid phase, heating removal ethyl alcohol obtains extracting solution；

2) when the extracting solution being concentrated into 1.5 times for being equivalent to Ginseng Root Powder quality, add the distilled water of 2 times of Ginseng Root Powder quality Mixing, then be added into obtained mixed liquor with ether defatting 1~2 time of distilled water equivalent volumes, stratification, by what is obtained Water layer with water layer equivalent water saturation extracting n-butyl alcohol 6~7 times, merge n-butanol phase, it is thick to obtain ginsenoside water bath method Product；

3) the ginsenoside crude product is dissolved with methanol, the acetone precipitation of 2~6 times of volumes of again with methanol, by collection Sediment is dried in vacuo at 70 DEG C, obtains the ginseng extract of mass concentration containing panaxoside 2.1%.

4. nervonic acid composition according to claim 1 or 2, which is characterized in that the extraction side of the siberian Ginseng P.E Method the following steps are included:

A. by 75% ethanol water of volumetric concentration of 6 times of quality of Manyprickle Acanthopanax Root 80 DEG C heating and refluxing extraction 2 times, every time 6h is extracted, is separated by solid-liquid separation, liquid phase is merged, heating removal ethyl alcohol obtains extracting solution；

B. the extracting solution is concentrated under reduced pressure, it is dry, obtain the siberian Ginseng P.E of mass content containing senticosus saponins 2.5%.

5. nervonic acid composition according to claim 1 or 2, which is characterized in that the extraction side of the ginkgo biloba p.e Method the following steps are included:

1. the ethanol water that Ginkgo Leaf and volumetric concentration are 70% is mixed and impregnates 10~12h, the soak that will be obtained At 25 DEG C, power is ultrasonically treated 25min under conditions of being 100W, obtains ultrasonic material；

2. the ultrasonic material is cleaned, extracting solution is obtained；

6. nervonic acid composition according to claim 1 or 2, which is characterized in that the nervonic acid is vegetative nerve acid, institute The raw material for stating vegetative nerve acid includes edible vegetable oil containing nervonic acid and its manufactured powdered oil or from the food containing nervonic acid The crystal nervonic acid separated with plant oil extract.

7. nervonic acid composition according to claim 1 or 2, which is characterized in that the purity of the phosphatidylserine is 50.0%~60.0%.

8. nervonic acid composition according to claim 1 or 2, which is characterized in that contain benzo furan in the celery oil It mutters letones；The benzofuranone substance accounts for 58.3% or more of celery oil quality, the Benzopyranone kind object The mass concentration that 3- normal-butyl -4,5- Dihydrobenzofuranes ketone in matter accounts for celery oil quality is 24.29%.

9. nervonic acid composition described in claim 1~8 any one is applied in preparing neuroprotection drug.

10. application according to claim 9, which is characterized in that the neuroprotection includes establishing collateral follow after headstroke Neuroprotection in terms of ring, the dysfunction and disabled aspect, headstroke that patient limb, language and spirit is reduced or avoided cause Hemiplegia, aphasia, in terms of peripheral neuropathy, promote intellectual development, delay brain aging, repair neural cell injury and/or In terms of prevention improves encephalopathy.