CN109105149A - A kind of ganoderma lucidum cultivation method - Google Patents
A kind of ganoderma lucidum cultivation method Download PDFInfo
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- CN109105149A CN109105149A CN201810953105.7A CN201810953105A CN109105149A CN 109105149 A CN109105149 A CN 109105149A CN 201810953105 A CN201810953105 A CN 201810953105A CN 109105149 A CN109105149 A CN 109105149A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
A kind of ganoderma lucidum cultivation method, prepare cultivation of glossy ganoderma culture medium using the raw material of following parts by weight: ramulus mori considers 60 parts~65 parts, 10 parts~15 parts of cereal bran, 15~20 parts of Chinese medicine slag, 3 parts~5 parts of gypsum, 3 parts~5 parts of corn flour, 0.02 part~0.08 part of magnesium sulfate, 0.02 part~0.08 part of magnesium sulfate, 0.02 part~0.03 part of monopotassium phosphate, 0.02 part~0.04 part of vitamin B1 to be worth doing, and 100 parts of medical stone powder.Ganoderma lucidum fruitbody polysaccharide is set to reach 0.65% or more using above-mentioned culture medium, content of polysaccharide in spores powder reaches 2.1% or more, much higher than the highest level of market ganoderma lucidum and content of polysaccharide in spores powder.
Description
Technical field
The present invention relates to cultivation of glossy ganoderma fields, and in particular to a kind of ganoderma lucidum cultivation method.
Background technique
Ganoderma lucidum is the rare traditional Chinese medicine of a kind of medicinal fungi and Chinese tradition.Modern medicine development discloses ganoderma lucidum
Many health cares and medical effect, and these effects and the 2 kinds of principle active component ganoderma lucidum polysaccharide and triterpenes of ganoderma lucidum are closely related.
Therefore, how significantly to improve cultivation technique, the content of polysaccharide and triterpenes components in the ganoderma lucidum fruitbody to improve production, at
For one of the hot spot in nearest ganoderma lucidum study on the industrialization
Summary of the invention
To solve the above-mentioned problems, the present invention provides a kind of ganoderma lucidum cultivation methods:
A kind of ganoderma lucidum cultivation method, comprising the following steps:
A, cultivation of glossy ganoderma culture medium: 60 parts of ramulus mori bits~65 parts, cereal bran is prepared using the raw material of following parts by weight
10 parts~15 parts, 15~20 parts of Chinese medicine slag, 3 parts~5 parts of gypsum, 3 parts~5 parts of corn flour, 0.02 part of magnesium sulfate~0.08 part, sulphur
0.02 part~0.08 part of sour magnesium, 0.02 part~0.03 part of monopotassium phosphate, 0.02 part~0.04 part of vitamin B1, medical stone powder 100
Part；100 parts of water are added in above-mentioned raw materials and are uniformly mixed, obtains culture medium, culture medium is respectively charged into multiple bacterium bags, and is covered
Ring seal lid；Bacterium bag is subjected to sterilization treatment to get culture medium bacterium bag, it is spare to put it into desinfection chamber cooling；
B, Liquid Strain of Ganoderma Lucidum is prepared using the raw material of following parts by weight: major ingredient: 15 parts~30 parts；Auxiliary material: glucose 1
Part~2 parts, 0.05 part~0.1 part of sodium dihydrogen phosphate；50 parts~80 parts of distilled water；The major ingredient is potato or corn or wheat；
The major ingredient is placed in distilled water after infusion and takes its juice, glucose, sodium dihydrogen phosphate are added in the juice, then in nothing
Shaking flask strain is added under the conditions of bacterium, and stirs, is kept for constant temperature incubation 3 days~5 days, temperature be 28 degrees Celsius to get
Liquid Strain of Ganoderma Lucidum；
C, the step B Liquid Strain of Ganoderma Lucidum prepared is inoculated in the culture medium bacterium bag of step A preparation；
D, by inoculated culture medium bacterium bag dislocation on the cultivating stand in mushroom room, the cultivating stand has multilayer, described
Culture medium bacterium bag is successively put from the bottom up, the temperature of mushroom room are as follows: and 18~25 degrees Celsius of the cultural hypha phase, fruiting phase 20~26
Degree Celsius, play 22~28 degrees Celsius of the powder phase；Humidity are as follows: cultural hypha phase 60%~70%, fruiting phase 75%~90% play the powder phase
65%~80%；Gas concentration lwevel are as follows: cultural hypha phase 0.03%~0.1%, fruiting phase former base are formed, stem growth phase
0.15%~0.3%, stem breaks up and plays the powder phase 0.03%~0.1%；Illuminance are as follows: 0 lux of cultural hypha phase, fruiting phase
And play 1000~2500 lux of powder phase；The illuminance is controlled by the black and white film being covered in outside the mushroom room, the black and white
Film area coverage is controlled by film-coiling device, and the black and white film has black side and white face, and white faces outwardly, black side court
It is interior；
E, when mycelia growth and maturity in the culture medium bacterium bag of step D, the capping of culture medium bacterium bag is opened, while thoroughly by one
The filter cloth of gas light transmission is covered on culture medium bacterium bag mouth, grows ganoderma lucidum fruitbody across filter cloth；To the powder stage is received, by the filter
The overturning of cloth surrounding is closed up, and is wrapped in the ganoderma lucidum fruitbody in the space that the filter cloth closes up, is made ganoderma lucidum fruitbody maturation bullet
The conidia powder of injection is wrapped in interior by the filter cloth.
Preferably, the mesh mesh number of the filter cloth is 200 mesh~500 mesh.
Preferably, the black and white film is made of phosphoenolpyruvate material, with a thickness of 80 microns~120 microns.
Preferably, the cultivating stand is made of steel, and height is at least 2 meters, between each layer between be divided into 20 centimetres~
25 centimetres, the angle of each layer bottom plate and horizontal plane is 0 degree~45 degree, and the cultivation capacity of the cultivating stand is 80 bags/square metre
~120 bags/square metre.
Preferably, the Chinese medicine slag is by composed of the following components by weight: 15 parts of Radix Astragali, 15 parts of Radix Codonopsis, 20 parts of dried orange peel,
5 parts of bone meal, 2 parts of dandelion.
The invention has the following beneficial effects: due to using ramulus mori and cereal bran, gypsum, pulverized limestone and medical stone powder
As culture medium for cultivating material, lignin, content of cellulose are high, are capable of providing sufficient nutrition, guarantee that ganoderma lucidum and conidia powder produce
While amount, functional component content is improved, so that ganoderma lucidum fruitbody polysaccharide is reached 0.65% or more, content of polysaccharide in spores powder reaches
2.1% or more, much higher than the highest level of market ganoderma lucidum and content of polysaccharide in spores powder.
Specific embodiment
The present invention will be further described below in conjunction with the embodiments.
A kind of embodiment: ganoderma lucidum cultivation method, comprising the following steps:
A, cultivation of glossy ganoderma culture medium is prepared using the raw material of following parts by weight: ramulus mori consider to be worth doing 60 parts, 10 parts of parts of cereal bran,
15 parts of Chinese medicine slag, 3 parts of parts of gypsum, 3 parts of corn flour, 0.02 part of magnesium sulfate, 0.02 part of magnesium sulfate, 0.02 part of monopotassium phosphate, dimension life
0.02 part of plain B1,100 parts of medical stone powder；100 parts of water are added in above-mentioned raw materials and are uniformly mixed, culture medium are obtained, culture medium
It is respectively charged into multiple bacterium bags, and lantern ring covers；Bacterium bag is subjected to sterilization treatment to get culture medium bacterium bag, is put it into sterile
Room cooling is spare, and the Chinese medicine slag is by composed of the following components by weight: 15 parts of Radix Astragali, 15 parts of Radix Codonopsis, 20 parts of dried orange peel, bone meal
5 parts, 2 parts of dandelion；
B, Liquid Strain of Ganoderma Lucidum is prepared using the raw material of following parts by weight: major ingredient: 15 parts~30 parts；Auxiliary material: glucose 1
Part, 0.05 part of sodium dihydrogen phosphate；50 parts of distilled water；The major ingredient is potato or corn or wheat；The major ingredient is placed in distillation
Its juice is taken in water after infusion, glucose, sodium dihydrogen phosphate are added in the juice, shaking flask bacterium is then aseptically added
Kind, and stir, it is kept for constant temperature incubation 3 days~5 days, temperature is 28 degrees Celsius to get Liquid Strain of Ganoderma Lucidum；
C, the step B Liquid Strain of Ganoderma Lucidum prepared is inoculated in the culture medium bacterium bag of step A preparation；
D, by inoculated culture medium bacterium bag dislocation on the cultivating stand in mushroom room, the cultivating stand has multilayer, described
Culture medium bacterium bag is successively put from the bottom up, the temperature of mushroom room are as follows: and 18~25 degrees Celsius of the cultural hypha phase, fruiting phase 20~26
Degree Celsius, play 22~28 degrees Celsius of the powder phase；Humidity are as follows: cultural hypha phase 60%~70%, fruiting phase 75%~90% play the powder phase
65%~80%；Gas concentration lwevel are as follows: cultural hypha phase 0.03%~0.1%, fruiting phase former base are formed, stem growth phase
0.15%~0.3%, stem breaks up and plays the powder phase 0.03%~0.1%；Illuminance are as follows: 0 lux of cultural hypha phase, fruiting phase
And play 1000~2500 lux of powder phase；The illuminance is controlled by the black and white film being covered in outside the mushroom room, the black and white
Film area coverage is controlled by film-coiling device, and the black and white film has black side and white face, and white faces outwardly, black side court
Interior, the black and white film is made of phosphoenolpyruvate material, with a thickness of 80 microns~120 microns.
E, when mycelia growth and maturity in the culture medium bacterium bag of step D, the capping of culture medium bacterium bag is opened, while thoroughly by one
The filter cloth of gas light transmission is covered on culture medium bacterium bag mouth, and the mesh mesh number of the filter cloth is 200 mesh~500 mesh, keeps ganoderma lucidum real
Body is grown across filter cloth；To the powder stage is received, filter cloth surrounding overturning is closed up, the ganoderma lucidum fruitbody is made to be wrapped in the filter
In the space that cloth closes up, the conidia powder for ejecting ganoderma lucidum fruitbody maturation is wrapped in interior by the filter cloth.The cultivating stand by
Steel are made, and height is at least 2 meters, between each layer between be divided into 20 centimetres~25 centimetres, the folder of each layer bottom plate and horizontal plane
Angle is 0 degree~45 degree, the cultivation capacity of the cultivating stand be 80 bag/square metre~120 bag/square metre.
A kind of embodiment: ganoderma lucidum cultivation method, comprising the following steps:
A, cultivation of glossy ganoderma culture medium is prepared using the raw material of following parts by weight: ramulus mori consider to be worth doing 65 parts, 15 parts of cereal bran, in
20 parts of the dregs of a decoction, 5 parts of gypsum, 5 parts of corn flour, 0.08 part of magnesium sulfate, 0.08 part of magnesium sulfate, 0.03 part of monopotassium phosphate, vitamin B1
0.04 part, 100 parts of medical stone powder；100 parts of water are added in above-mentioned raw materials and are uniformly mixed, obtains culture medium, culture medium is distinguished
It is fitted into multiple bacterium bags, and lantern ring covers；Bacterium bag is subjected to sterilization treatment to get culture medium bacterium bag, it is cold to put it into desinfection chamber
Spare, the Chinese medicine slag is by composed of the following components by weight: 15 parts of Radix Astragali, 15 parts of Radix Codonopsis, 20 parts of dried orange peel, 5 parts of bone meal,
2 parts of dandelion；
B, Liquid Strain of Ganoderma Lucidum is prepared using the raw material of following parts by weight: major ingredient: 15 parts~30 parts；Auxiliary material: glucose 1
Part, 0.05 part of sodium dihydrogen phosphate；50 parts of distilled water；The major ingredient is potato or corn or wheat；The major ingredient is placed in distillation
Its juice is taken in water after infusion, glucose, sodium dihydrogen phosphate are added in the juice, shaking flask bacterium is then aseptically added
Kind, and stir, it is kept for constant temperature incubation 3 days~5 days, temperature is 28 degrees Celsius to get Liquid Strain of Ganoderma Lucidum；
C, the step B Liquid Strain of Ganoderma Lucidum prepared is inoculated in the culture medium bacterium bag of step A preparation；
D, by inoculated culture medium bacterium bag dislocation on the cultivating stand in mushroom room, the cultivating stand has multilayer, described
Culture medium bacterium bag is successively put from the bottom up, the temperature of mushroom room are as follows: and 18~25 degrees Celsius of the cultural hypha phase, fruiting phase 20~26
Degree Celsius, play 22~28 degrees Celsius of the powder phase；Humidity are as follows: cultural hypha phase 60%~70%, fruiting phase 75%~90% play the powder phase
65%~80%；Gas concentration lwevel are as follows: cultural hypha phase 0.03%~0.1%, fruiting phase former base are formed, stem growth phase
0.15%~0.3%, stem breaks up and plays the powder phase 0.03%~0.1%: illuminance are as follows: 0 lux of cultural hypha phase, fruiting phase
And play 1000~2500 lux of powder phase；The illuminance is controlled by the black and white film being covered in outside the mushroom room, the black and white
Film area coverage is controlled by film-coiling device, and the black and white film has black side and white face, and white faces outwardly, black side court
Interior, the black and white film is made of phosphoenolpyruvate material, with a thickness of 80 microns~120 microns.
E, when mycelia growth and maturity in the culture medium bacterium bag of step D, the capping of culture medium bacterium bag is opened, while thoroughly by one
The filter cloth of gas light transmission is covered on culture medium bacterium bag mouth, and the mesh mesh number of the filter cloth is 200 mesh~500 mesh, keeps ganoderma lucidum real
Body is grown across filter cloth；To the powder stage is received, filter cloth surrounding overturning is closed up, the ganoderma lucidum fruitbody is made to be wrapped in the filter
In the space that cloth closes up, the conidia powder for ejecting ganoderma lucidum fruitbody maturation is wrapped in interior by the filter cloth.The cultivating stand by
Steel are made, and height is at least 2 meters, between each layer between be divided into 20 centimetres~25 centimetres, the folder of each layer bottom plate and horizontal plane
Angle is 0 degree~45 degree, the cultivation capacity of the cultivating stand be 80 bag/square metre~120 bag/square metre.
Obviously, the above embodiment of the present invention is intended to be merely illustrative of the present example, and not to the present invention
Embodiment restriction.For those of ordinary skill in the art, it can also make on the basis of the above description
Other various forms of variations or variation.There is no need and unable to give poor example to all embodiments.And these belong to this
The connotation changes and variations that derived from of invention still fall within protection scope of the present invention.
Claims (5)
1. a kind of ganoderma lucidum cultivation method, it is characterised in that the following steps are included:
A, prepare cultivation of glossy ganoderma culture medium using the raw material of following parts by weight: ramulus mori considers 60 parts~65 parts, 10 parts of cereal bran to be worth doing
~15 parts, 15~20 parts of Chinese medicine slag, 3 parts~5 parts of gypsum, 3 parts~5 parts of corn flour, 0.02 part of magnesium sulfate~0.08 part, magnesium sulfate
0.02 part~0.08 part, 0.02 part~0.03 part of monopotassium phosphate, 0.02 part~0.04 part of vitamin B1,100 parts of medical stone powder；
100 parts of water are added in above-mentioned raw materials and are uniformly mixed, obtains culture medium, culture medium is respectively charged into multiple bacterium bags, and lantern ring
Capping；Bacterium bag is subjected to sterilization treatment to get culture medium bacterium bag, it is spare to put it into desinfection chamber cooling；
B, Liquid Strain of Ganoderma Lucidum is prepared using the raw material of following parts by weight: major ingredient: 15 parts~30 parts；Auxiliary material: 1 part of glucose~
2 parts, 0.05 part~0.1 part of sodium dihydrogen phosphate；50 parts~80 parts of distilled water；The major ingredient is potato or corn or wheat；By institute
It states major ingredient and is placed in distilled water after infusion and take its juice, glucose, sodium dihydrogen phosphate are added in the juice, then in sterile item
Shaking flask strain is added under part, and stirs, is kept for constant temperature incubation 3 days~5 days, temperature is 28 degrees Celsius to get ganoderma lucidum
Liquid spawn；
C, the step B Liquid Strain of Ganoderma Lucidum prepared is inoculated in the culture medium bacterium bag of step A preparation；
D, by inoculated culture medium bacterium bag dislocation on the cultivating stand in mushroom room, the cultivating stand has multilayer, the culture
Base bacterium bag is successively put from the bottom up, the temperature of mushroom room are as follows: 18~25 degrees Celsius of the cultural hypha phase, fruiting phase 20~26 is Celsius
Degree plays 22~28 degrees Celsius of the powder phase；Humidity are as follows: cultural hypha phase 60%~70%, fruiting phase 75%~90% play the powder phase 65%
~80%；Gas concentration lwevel are as follows: cultural hypha phase 0.03%~0.1%, fruiting phase former base are formed, stem growth phase
0.15%~0.3%, stem breaks up and plays the powder phase 0.03%~0.1%；Illuminance are as follows: 0 lux of cultural hypha phase, fruiting phase
And play 1000~2500 lux of powder phase；The illuminance is controlled by the black and white film being covered in outside the mushroom room, the black and white
Film area coverage is controlled by film-coiling device, and the black and white film has black side and white face, and white faces outwardly, black side court
It is interior；
E, when mycelia growth and maturity in the culture medium bacterium bag of step D, the capping of culture medium bacterium bag is opened, while ventilative saturating by one
The filter cloth of light is covered on culture medium bacterium bag mouth, grows ganoderma lucidum fruitbody across filter cloth；To the powder stage is received, by the filter cloth four
Week overturning is closed up, and is wrapped in the ganoderma lucidum fruitbody in the space that the filter cloth closes up, is ejected ganoderma lucidum fruitbody maturation
Conidia powder be wrapped in by the filter cloth in.
2. ganoderma lucidum cultivation method according to claim 1, it is characterised in that: the mesh mesh number of the filter cloth be 200 mesh~
500 mesh.
3. ganoderma lucidum cultivation method according to claim 1 or 2, it is characterised in that: the black and white film is by phosphoenolpyruvate third
Ketone acid material is made, with a thickness of 80 microns~120 microns.
4. ganoderma lucidum cultivation method according to claim 3, it is characterised in that: the cultivating stand is made of steel, height
At least 2 meters, between each layer between be divided into 20 centimetres~25 centimetres, the angle of each layer bottom plate and horizontal plane is 0 degree~45 degree,
The cultivation capacity of the cultivating stand be 80 bag/square metre~120 bag/square metre.
5. ganoderma lucidum cultivation method according to claim 3, it is characterised in that: the Chinese medicine slag is by by weight by with the following group
It is grouped as: 15 parts of Radix Astragali, 15 parts of Radix Codonopsis, 20 parts of dried orange peel, 5 parts of bone meal, 2 parts of dandelion.
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Cited By (2)
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| CN114009274A (en) * | 2021-12-02 | 2022-02-08 | 浙江寿仙谷医药股份有限公司 | Moisturizing synergistic ganoderma lucidum planting matrix and preparation method and efficient recycling method thereof |
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Application publication date: 20190101 |