CN105925616A - Fruit and vegetable culture and probiotics fermentation of medicinal and edible fungi - Google Patents

Fruit and vegetable culture and probiotics fermentation of medicinal and edible fungi Download PDF

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Publication number
CN105925616A
CN105925616A CN201610285374.1A CN201610285374A CN105925616A CN 105925616 A CN105925616 A CN 105925616A CN 201610285374 A CN201610285374 A CN 201610285374A CN 105925616 A CN105925616 A CN 105925616A
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fruit
vegerable
macro fungi
lactobacillus
fungi
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刘东波
王蕾
康信聪
陈晓明
张思
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Liaoning Shengqi Haotian Biomedicine Technology Co Ltd
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Liaoning Shengqi Haotian Biomedicine Technology Co Ltd
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Priority to CN201610285374.1A priority Critical patent/CN105925616A/en
Publication of CN105925616A publication Critical patent/CN105925616A/en
Priority to PCT/CN2017/082209 priority patent/WO2017186146A1/en
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Abstract

The invention relates to a fruit and vegetable culture product of large fungi, wherein fruits and vegetables are free from the additional addition of a protein ingredient. The invention also relates to a finished product obtained through performing further probiotics fermentation on the culture product, as well as production methods of the culture product and the finished product.

Description

The fruit and vegerable of medical edible fungal are cultivated and probiotics fermention
Technical field
The fruit and vegerable that the present invention relates to macro fungi are cultivated and probiotics fermention.
Background technology
There is now multiple macro fungi and can pass through liquid fermentation, obtain within the short time (e.g., from about 5～45 days) Substantial amounts of mycelium, and in fermentation liquid, comprise its multiple medicinal ingredient.But culture medium used is general all Requirement is especially added with nitrogen source such as protein component, and the culture medium of such as Ganoderma is often required to add soybean cake or egg White peptone, the culture medium of Cordyceps adds dried silkworm chrysalis meal and yeast extract etc..
The most about making the document of ferment of fruit and vegerable, there is the strain utilizing fruit and vegerable to carry to carry out nature and send out Ferment, also have adding what probiotic bacteria fermented after fruit and vegerable sterilizing, more have and additionally add in ferment product Chinese herbal medicine or its extracting solution are to obtain more diversified nutrition.The preparation process of these ferment is mostly relatively more multiple Miscellaneous, the cycle is the longest.Up to now, applicant finds no and only cultivates as culture medium with fruit and vegerable Macro fungi, and the ferment comprising macro fungi and active component thereof is made further by probiotics fermention Element.The application is improved in these areas.
Summary of the invention
Unless otherwise specified, all terms the most used herein have logical with those of ordinary skill in the art The implication that the implication that understands is identical.
" macro fungi " refers to herein, edible after do not cause that human body is poisoned, to form naked eyes visible The fungus of sporophore.Edible fungus and medicinal fungi is included in the category of described macro fungi, so this The macro fungi of literary composition is referred to as medical edible fungal.Medicinal fungi herein e.g., in pharmacopoeia of each country Listed macro fungi.
In a particular embodiment, macro fungi described herein is breaking cellular wall fungus, i.e. described herein large-scale In fungus, the surface texture that plant cell wall or insect body wall etc. are hard can be produced the fungus of shell-broken effect. Referred to herein as shell-broken effect, it is not required that making wall be completely severed, as long as making to start a leak on wall, including Thing is easier to outwards discharge.
The main component of known plants cell wall has cellulose, hemicellulose and pectin.Correspondingly, can produce Raw cellulase, hemicellulase and/or the macro fungi of pectase, be all can be to plant cell wall Cause shell-broken effect described herein, therefore broadly fall into breaking cellular wall fungus as herein described.
It is true that have many macro fungis can wooden such as trees on grow, they can break through hard wood Matter surface, is bonded to wherein, illustrates that they all have shell-broken effect as herein described, therefore, these energy The many cells fungus (being called for short " lignicolous fungi ") of growth on wooden belongs to breaking cellular wall fungus as herein described, Object lesson has: Ganoderma (Ganoderma), Poria (Poria cocos), Polyporus (Grifola umbellata), Etc..Lignicolous fungi include wood parasitical fungi and wood saprophytic fungus, wood parasitical fungi example such as Polyporus, The example such as Ganoderma, Poria of wood saprophytic fungus.
In addition to the plant cells such as trees have rigid surface, insecticide and larva thereof also have hard in body wall Cellulose, chitin composition, major part fungus cannot grow.But some fungus, energy infected insect (as Lepidoptera) larva or pupa so that the insecticide of larva → pupa → adult growth stage to be experienced terminates In larva or the stage of pupa, such fungus then utilizes the health of insect larvae or pupa to grow for nutrition. Cordyceps is exactly such fungus.In this article, " Cordyceps " refers to sensu lato Cordyceps (Cordyceps) Fungus, they parasitize insecticide, polypide are become the Bombyx Batryticatus of full mycelia, bear button from Bombyx Batryticatus front end Shape or club-shaped Stroma.Cordyceps sinensis fungus the most reported more than kind more than 400, be widely known by the people most Including Cordyceps (Cordyceps sinensis), Cordyceps militaris (L.) Link. (Cordyceps militaris) etc..Cordyceps energy Break through insect body wall, illustrate to produce corresponding breaking cellular wall material, such as cellulase, chitinase etc., Therefore breaking cellular wall fungus as herein described is fallen within.
Correspondingly, in a particular embodiment, macro fungi described herein includes lignicolous fungi and Cordyceps, More specifically, breaking cellular wall fungus described herein includes lignicolous fungi and Cordyceps.
In a more preferred embodiment, macro fungi described herein is selected from Ganoderma, Poria, Polyporus, winter Worm summer grass and/or Cordyceps militaris (L.) Link..Even more preferably from embodiment in, breaking cellular wall fungus described herein selected from spirit Sesame, Poria, Polyporus, Cordyceps and/or Cordyceps militaris (L.) Link..
Known macro fungi mostly has spore, mycelium, a sporophore three phases, wherein mycelium be into The multicell that row is nourished and grown, shows as being assembled by the visible long filamentous cell of numerous microscopic level forming Macroscopic thalline.Mycelium is usually and absorbs nutrition from environment energetically, raw for subsequent child entity Length is stocked.Therefore, the mycelial growth of macro fungi is the highest to the nutritional requirement of environment.When When environmental condition is unsuitable for growth, the mycelium of some macro fungi is it is possible to change into hypopus, such as By " sclerotium " hard outside pine mycelium compact winding, interior, Polyporus, Poria etc. have the sclerotium stage. When sclerotium runs into suitable environmental condition, new vegetative hyphae can be germinated or grow up to sporophore.
For Ganoderma, the Ganoderma described in Chinese Pharmacopoeia is primarily referred to as its sporophore, common for umbrella. Sporophore discharges Ganoderma spore, and this is the sexual cell of Ganoderma in fact.Ganoderma spore runs into suitable ring Mycelia can be sprouted in border.Numerous mycelia assemble arrangement, form mycelium.These mycelia are as the root system one of plant Sample, constantly absorbs from surrounding and stores nutrition, doing the accumulation of necessity for follow-up sporophore growth. The nutrient inventory of Ganoderma mycelium typically can exceed 3-5 times than sporophore, the most.Many with Ganoderma As a example by sugar, mycelial polyoses content is up to about 15%, and the polyoses content of sporophore only has general 3%. And, Ganoderma mycelium is soft, and cell membrane is relatively thin, and the abundant nutrition composition that it includes is easier to inhale into human body Receive.By contrast, Ganoderma sporophore is harder, and its nutrition is difficult to be absorbed by the body relatively.
As for Cordyceps, as already described above, it, when polypide endoparasitism, is mycelium, when it breaks through worm When body forms grass, it is put into the sporophore stage.
" fruit and vegerable " refer to one or more fruit, one or more vegetable or one or more fruit herein With the mixture of one or more vegetable, " fruit " therein and " vegetable " are all referring in daily life General sense.
" ferment (fermentation) " herein and refer to inoculate in plant or its material such as fruit, stem and leaf Microorganism carries out the process cultivated.Due to the vital movement of microorganism, can be by carbohydrate-modifying The material such as alcohol and organic acid, it is possible to so that the composition (such as nutritional labeling) contained by plant changes, example Such as easier release, activity is higher, or becomes the form being more beneficial for being absorbed by the body.
Herein " ferment (ferment nutrients) " to refer to that plant obtains through probiotics fermention multiple The mixture of bioactive substance, comprising: fermentation participates in bacterium, participates in for the food materials fermented, fermentation Bacterium or the active component of food materials, the most multiple enzyme (enzyme), also include the composition modified because of fermentation, The most active higher or be more beneficial for absorb composition, etc..In a particular embodiment, by fruit And/or the culture medium of vegetable composition carries out probiotics fermention, obtain " fruit-vegerable ferment ".Described here " fruit and vegerable ", refer to fruits and vegetables described in daily life, there is no other particular meaning.
The fruit and vegerable of macro fungi are cultivated
Before the application, macro fungi can carry out liquid culture, but has no only with fruit and/or vegetable For culture medium, the composition of rich in proteins, such as peptone, soybean cake etc. at least will be added.This can Can be because, as already described above, the nutritional requirement of environment is usually compared by the mycelial growth of macro fungi Height, meeting dormancy when environment is not suitable for, therefore, if the most additionally adding nutrient protein composition, simple fruit and vegerable Trophic level (such as protein content) may be unsatisfactory for the mycelial high nutritional requirement of macro fungi.
On the other hand, previously herein, macro fungi either liquid culture or solid culture, target Sporophore to be obtained, there are no simple cultured mycelia and is directly used as final products.But The time that sporophore growth spends is the longest, such as, and the sporophore growth mycelial growth to be compared of Ganoderma Usually have more the time of about 5 times, Cordyceps from polypide become cursive script be also required to expend 9～10 months left The right time.
The present inventor macro fungi is inoculated into that be made up of fruit and/or vegetable, the most additionally add protein Culture medium in cultivate, have been surprisingly found that macro fungi can carry out good growth, especially mycelial growth. (technical staff can determine according to incubation time and/or morphological feature by rule of thumb easily: is trained The macro fungi supported only exists in mycelium stage, not up to sporophore stage).After tested, gained is cultivated Thing has macro fungi respective given activity composition；Advantageously, gained culture can be further Carry out probiotics fermention, produce the ferment product that nutritional labeling is various and is easily absorbed by the human body.
Fruit and vegerable culture medium as herein described by one or more fruit, one or more vegetable or one or more Fruit forms with the mixture of one or more vegetable.In culture medium, the kind number of fruit and/or vegetable does not limits. In a specific embodiment, for same macro fungi, attempt few to 3 kinds and up to 16 kinds fruits The mixture of vegetables makes culture medium, and result all detects various active index, thus indicates large-scale very The good growth of bacterium.Therefore, on the premise of without counter-example, fruit and vegerable culture medium herein can be according to locality Prepared by the combination in any of the obtainable fruits and vegetables of this season, it is also possible to the combination different geographical same time Prepared by the combination in any of obtainable various fruits and vegetable.
Fruit and vegerable culture medium as herein described can be eaten by adding tablet vinegar or edible sodium hydroxide solution etc. By pH adjusting agent, be adjusted to the pH of culture medium to be suitable to inoculated growth the suitableeest in the range of, The suitableeest described scope is the optimum pH scope of corresponding strain known in the art.Such as, for Ganoderma, For the cultivation of Cordyceps, can be pH5.0～8.0.
The fruit and vegerable of macro fungi as herein described are cultivated, in the range of the optimum growth temperature of this macro fungi Carrying out, described optimum growth temperature scope is the optimum growth temperature scope of corresponding strain known in the art. Such as 7～30 DEG C, preferably 18～28 DEG C.
The fruit and vegerable of macro fungi as herein described are cultivated, and the length of its incubation time depends on that each strain is raw Time needed for time needed for length, especially mycelial growth, but without the most accurate.Such as, institute State fruit and vegerable to cultivate and can carry out 3～45 days, preferably 5～15 days, or described in the range of any natural law. Described incubation time differs 1～3 day, and the active component content of final products affects little (data do not show Show).
The fruit and vegerable of macro fungi as herein described are cultivated, and can carry out, at this by the form of fermentation tank stirring In the case of Zhong, the speed of fermentation tank stirring is also that those skilled in the art's characteristic based on selected strain can Determine, such as 50～250rpm, preferably 90～180rpm.
The fruit and vegerable cultured products of the macro fungi of the application, had both comprised the active component of macro fungi, had also wrapped Active component containing fruit and vegerable.Such as, when the macro fungi of the application is breaking cellular wall fungus, owing to breaking cellular wall is true The shell-broken effect of bacterium so that the fruit and vegerable cultured products of breaking cellular wall fungus, the most only mycelium of fungus and battalion thereof Form point, and fruit and vegerable also because being discharged its active substance more by breaking cellular wall.With inoculate non-breaking cellular wall true The fruit and vegerable of bacterium are cultivated and are compared, the fruit and vegerable cultured products of the application before also not carrying out probiotics fermention the most Rendered go out fruit and vegerable composition more high activity, therefore become product more nutritious to human body.
In preferred embodiments, macro fungi as herein described is Ganoderma.
In another preferred embodiment, macro fungi as herein described is Cordyceps militaris (L.) Link..
Probiotic bacteria (probiotics) ferments
Before the application, it is relatively common for carrying out food fermentation with probiotic bacteria.That commonly uses for this is prebiotic Bacterium has, yeast (yeast), lactobacillus (Lactobacillus), bacillus bifidus (Bifidobacterium), Leukonid (Leuconostoc), streptococcus (Streptococcus), Lactococcus (Lactococcus), acetic acid Bacillus (Acetobacterium) etc..Described yeast such as saccharomyces cerevisiae (Saccharomyces And the saccharomyces (Saccharomyces) such as saccharomyces ellipsoideus (Saccharomyes eerevisiae) cerevisiae) Yeast, pichia pastoris phaff (Pichia pastoris), Pichia ohmeri (Pichia ohmeri), film The yeast of the pichias (Pichia) such as uncut jade Pichia sp. (Pichia membranaefaciens), the abnormal Chinese The yeast of the Hansenulas (Hansenula) such as inferior yeast (Hansenula anomala).Described lactobacillus Such as, Lactobacillus buchneri (Lactobacillus buchneri), lactobacillus panis (Lactobacillus panis), Bacillus acidophilus (Lactobacillus acidophilus), Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus fermenti (Lactobacillus fermenti), saliva breast bar Bacterium (Lactobacillus salivarius), Lactobacillus brevis (Lactobacillus brevis), light green breast bar Bacterium (Lactobacillus veridescens), lactobacillus delbruockii subspecies bulgaricus (Lactobacillus delbrueckii bulgaricus), rhamnose breast bar Bacterium (Lactobacillus rhamnosus), lactobacillus cellobiosas (Lactobacillus cellobiosus), L. casei casei (Lactobacillus casei subsp casei), lactobacillus helveticus are the most ancient Special subspecies (Lactobacillus helveticus subsp.jugurti), Jansen lactobacillus (Lacticum Jansen), Lactobacillus pentosus (Lactobacillus pentosus), etc..Described bifid Bacillus such as, bifidobacterium adolescentis (Bifidobacterium adolescents), bifurcation controlling (Bifidobacterium bifidum), bifidobacteria infantis (Bifidobacterium infantis), length pair Discrimination bacillus (Bifidobacterium longum), etc..Described acetobacter such as Acetobacter pasteurianus (Acetobacter pasteuranus), stench acetobacter (Acetobacter rancens) etc..Other are prebiotic Bacterium also has: goldbeater's skin bright beading coccus (Leuconostoc mesenteroides aureus), streptococcus salivarius Thermophilous subspecies (Streptococcus salivarius subsp.thermophilus), lactis subsp. cremoris (Lactococcus lactis subsp.Cremoris), etc..
Correspondingly, the application further relates to a kind of fermentation process, and it is by the fermentation of macro fungi and probiotic bacteria Fementative composition.In a more particular embodiment, the application relates to the fermentation first carrying out macro fungi, Carry out the fermentation of probiotic bacteria again.In a more particular embodiment, the probiotics fermention yeast of the application Bacterium, lactobacillus and acetobacter are carried out.In a more particular embodiment, the probiotic bacteria of the application is sent out Ferment is to carry out step by step, it is preferable that first carries out the mixed fermentation of yeast and lactobacillus, then carries out vinegar The fermentation of acidfast bacilli.The yeast of the application, lactobacillus and acetobacter can together with add, enter Row one-step fermentation rather than be dividedly in some parts, batch fermentation, so can be greatly saved ferment Production Time. In a more particular embodiment, the probiotic bacteria of the application is saccharomyces cerevisiae, Lactobacillus buchneri and bar Family name's acetobacter.
The application is using the fruit and vegerable cultured products of above-mentioned macro fungi as culture medium, and inoculation is above-mentioned further Common probiotic bacteria is cultivated, available macro fungi-fruit-vegerable ferment, such as Ganoderma-ferment or Cordyceps Ferment.Applicants have discovered that, carry out sending out compared to the fruit and vegerable direct inoculation probiotic bacteria not inoculating macro fungi Ferment, the application first inoculate macro fungi cultivate after fruit and vegerable when probiotics fermention, condition of culture is wanted Ask and there is no significant change.
In preferred embodiments, the macro fungi that can share same or similar condition of culture can also be together Fruit and vegerable culture medium is cultivated, preferably further carries out probiotics fermention so that final product comprises More than one macro fungi and/or its active component.
In another embodiment, in the fruit and vegerable of probiotics fermention, comprise macro fungi and/or its composition. In yet another embodiment, the fruit and vegerable inoculation probiotic bacteria comprising macro fungi and/or its composition is carried out ferment Fermentation.So, another embodiment herein relates to the method for ferment fermentation, is in fruit and vegerable culture medium, First inoculation macro fungi grows, and such as mycelial growth inoculates probiotic bacteria and ferments.Preferably Ground, described method is not related to extract the step of macro fungi active component, i.e. described method is without greatly Extraction macro fungi active component after type fungus growth step, but the growth of macro fungi and probiotic bacteria Fermentation can carry out successively in same culture systems or installation for fermenting.
The fruit and vegerable culture medium of the application is simple for production, and all of fruit and/or vegetable raw-material only need to be cleaned, nothing Need to carry out disinfection or sterilizing, this is at least partly owing to fruit and vegerable cultivation herein is by artificial vaccination strain It is oriented cultivation, is not as prior art, carry out merely with the strain carried natural in fruit and vegerable Fermentation, in the case of dominant growth, is not afraid of the pollution carrying strain in fruit and vegerable raw material.
In the embodiment of a concrete example, the present processes comprises the steps:
1, by fruit and/or the vegetable of wash clean, stripping and slicing, homogenate, pour in fermentation tank；
2, pH value is adjusted to 5.0～7.0 with tablet vinegar or edible sodium hydroxide solution,；
3, after cooling, accessing large-size fungus species, control mixing speed is 90～180rpm, temperature is 18～28 DEG C, fermentation 5～15 days；
4, access 1～3 ‰ (m/m) yeast (million grades of viable counts), add 1～3 ‰ (m/m) lactic acid bacteria (hundred Ten thousand grades of viable counts), add 1～3 ‰ (m/m) acetobacter (million grades of viable counts), seal, in 25～38 DEG C Quiescent culture 5～15 days under environment；
5, filtering, seasoning, room temperature keeps in Dark Place, and obtains macro fungi-fruit-vegerable ferment product.
With more specifically illustrating, the application relates to
1. a compositions, is the macro fungi product cultivated through fruit and vegerable, and fruit and vegerable culture medium therein is by water Fruit and/or vegetable form and without the protein component additionally added.
2. the compositions of 1, wherein the macro fungi in product is mycelium form.
4., in the compositions of first any one, macro fungi therein is breaking cellular wall fungus.
5., in the compositions of first any one, macro fungi therein is lignicolous fungi and/or Cordyceps.
6., in the compositions of first any one, macro fungi therein is artificial culture.
7., in the compositions of first any one, macro fungi therein is selected from Ganoderma, Polyporus, Poria, winter worm Summer grass and/or Cordyceps militaris (L.) Link..
8. a compositions, is the compositions one of item 1-7 Suo Shu to be carried out probiotics fermention further obtain The product arrived.
9. the compositions of 8, probiotic bacteria therein is selected from following one or more: sugar yeast, finish red ferment Mother, Hansenula yeast, lactobacillus, bacillus bifidus, leukonid, streptococcus, Lactococcus, acetic acid bar Bacterium.
10. the compositions of 8, probiotic bacteria therein is selected from following one or more: saccharomyces cerevisiae, ellipse Torula, pichia pastoris phaff, Pichia ohmeri, film uncut jade Pichia sp., Hansenula anomala, Lactobacillus buchneri, lactobacillus panis, bacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermenti, saliva breast Bacillus, Lactobacillus brevis, light green lactobacillus, lactobacillus delbruockii subspecies bulgaricus, lactobacillus rhamnosus, Lactobacillus cellobiosas, L. casei casei, lactobacillus helveticus Yue Gute subspecies, Jansen breast bar Bacterium, Lactobacillus pentosus), Acetobacter pasteurianus, stench acetobacter, bifidobacterium adolescentis, bifurcated double Discrimination bacillus, bifidobacteria infantis, bifidobacterium longum, goldbeater's skin bright beading coccus, saliva chain coccus thermophilous Asia Plant and lactis subsp. cremoris.
The compositions of 11. 8, probiotic bacteria therein is saccharomyces cerevisiae, lactobacillus and acetobacter.
The compositions of one of 12. 8-11, each of which million grades of viable count of selection of described probiotic bacteria Bacterium solution is also inoculated by the amount that total raw material mass fraction is 1～3 ‰.
13. methods cultivating macro fungi, are with being only made up of fruit and/or vegetable and without additionally adding The fruit and vegerable culture medium of the protein component added is cultivated.
The method of 14. 13, its condition of culture is: with the mixing speed of 90～180rpm, at 18～28 DEG C, Cultivate 5～15 days.
15. ferment manufacture methods, are in fruit and vegerable culture medium, and first inoculation macro fungi is cultivated, then Inoculation probiotic bacteria ferments, and described fruit and vegerable culture medium is made up of fruit and/or vegetable and without additionally The protein component added.
The method of one of 16. 13-15, is wherein not related to extract the step of macro fungi active component.
The method of one of 17. 13-16, it is mycelial growth that macro fungi therein is cultivated.
The method of one of 18. 13-17, macro fungi therein is breaking cellular wall fungus.
The method of one of 19. 13-18, macro fungi therein is lignicolous fungi or Cordyceps.
The method of one of 20. 13-19, macro fungi therein is artificial culture.
The method of one of 21. 13-20, macro fungi therein is selected from Ganoderma, Polyporus, Poria, winter Worm summer grass and/or Cordyceps militaris (L.) Link..
The method of one of 22. 13-21, probiotic bacteria therein is selected from following one or more: sugar yeast, Pichia sp., Hansenula yeast, lactobacillus, bacillus bifidus, leukonid, streptococcus, Lactococcus, Acetobacter.
The method of 23. 22, probiotic bacteria therein is selected from following one or more: saccharomyces cerevisiae, ellipse Yeast, pichia pastoris phaff, Pichia ohmeri, film uncut jade Pichia sp., Hansenula anomala, cloth Family name's lactobacillus, lactobacillus panis, bacillus acidophilus, Lactobacillus plantarum, Lactobacillus fermenti, saliva breast bar Bacterium, Lactobacillus brevis, light green lactobacillus, lactobacillus delbruockii subspecies bulgaricus, lactobacillus rhamnosus, Lactobacillus cellobiosas, L. casei casei, lactobacillus helveticus Yue Gute subspecies, Jansen breast bar Bacterium, Lactobacillus pentosus), Acetobacter pasteurianus, stench acetobacter, bifidobacterium adolescentis, bifurcated double Discrimination bacillus, bifidobacteria infantis, bifidobacterium longum, goldbeater's skin bright beading coccus, saliva chain coccus thermophilous Asia Plant and lactis subsp. cremoris.
The method of 24. 22, probiotic bacteria therein is saccharomyces cerevisiae, lactobacillus and acetobacter.
The method of one of 25. 22-24, each of which million grades of viable count of selection of described probiotic bacteria Bacterium solution is also inoculated by the amount that total raw material mass fraction is 1～3 ‰.
The following examples are the specific descriptions to the application, and these embodiments are intended to enter the application One step explanation, it is impossible to be interpreted as that the protection domain of invention is had any restriction, those skilled in the art's base Improvement and the adjustment made in present context fall within shielded scope.
Embodiment
Detection method
Lipase activity: detect according to CNS GB/T 23535-2009.
Amylase activity: the α-amylase solution of alpha-amylase inhibitor with equivalent is incubated 15 at 37 DEG C Minute, the soluble starch solution reaction of addition 2% 5 minutes, add DNS (3,5-dinitrosalicylic Acid) reagent, boiling water bath cools down with flowing water rapidly after 5 minutes, carries out colorimetric method for determining (Zhao with 470nm wavelength Rong etc., Chinese patent medicine, 2013,35 (3): 573-576).
Oxidation resistance: DPPH Scavenging activity, superoxide anion Scavenging activity, Hydroxyl radical-scavenging energy Power is pressed " preliminary study of Hylocereus undatus ferment biological activity ", Dong Yinmao etc., food science and technology, and 2009, Method described in 34 (3): 192-196 detects.
Ganoderma triterpenoids: after sample solution heating is made solvent volatilization, add 5% vanillin of fresh configuration- Perchloric acid solution, 70 DEG C of water-baths 15 minutes, it is cooled to room temperature with flowing water subsequently, measures 550nm wavelength Absorbance (bow Xiao Feng etc., research and development of natural products, 2006,18:825-829).
Cordycepin: according to the Ministry of Agriculture about cordycepin in cordyceps product and the high performance liquid chromatography of adenosine Method bioassay standard NY/T2116-2012 detects.
Cordycepic acid: have another name called PEARLITOL 25C, therefore according to the Ministry of Agriculture about edible fungus quality surveillance mark In quasi-NY/T 2279-2012, the standard of mannitol detects.
Embodiment 1
Fruit and vegerable are cultivated: weigh blue berry 50g, Fructus Armeniacae Mume 10g, Fructus Rubi 50g, kiwi fruit 100g, Fructus Myricae rubrae 100 G, Fructus Fragariae Ananssae 150g, Fructus Pruni pseudocerasi 80g, Fructus Jujubae 100g, Citrullus vulgaris 200g, Fructus Mangifera Indicae 150g, Hylocereus undatus 200 G, Fructus actinidiae chinensis 200g, Fructus Mali pumilae 200g, Fructus Musae 100g, Fructus Pruni salicinae 200g, hami melon 300g, homogenate, With edible sodium hydroxide solution tune pH for 6.8, sterilizing, obtain No. 1 fruit and vegerable culture medium.Access No. 1 Ganoderma The mycelia that bacterium (purple light Ganoderma, CGMCC numbering 5.160 belong to Ganoderma) activates through slant medium, puts Cultivate 15 days in 120rpm, 20 DEG C of shaking tables.Measurement result is shown in Table 1.
Probiotics fermention: add 1 ‰ (weight) saccharomyces cerevisiae (CGMCC numbering 2.3888, million grades of viable bacterias Number), 1 ‰ (weight) Lactobacillus buchneri (CGMCC numbering 1.3114, million grades of viable counts) and 1 ‰ (m/m) Acetobacter (acetobacter Shanghai makes 1.01, brewages a factory, million grades of viable counts purchased from Shanghai), 28 DEG C close Close standing 7 days.Measurement result is shown in Table 1.
Filter off fruit and vegerable slag and undissolved mycelium, obtain clear filtrate, for Ganoderma-fruit ferment product 1, Room temperature keeps in Dark Place.
Embodiment 2
Fruit and vegerable are cultivated: weigh Fructus Mali pumilae 1200g, Hylocereus undatus 800g, Rhizoma Nelumbinis 400g, and homogenate, with edible hydrogen Sodium hydroxide solution adjusts pH to be 6.8, and sterilizing obtains fruit and vegerable culture medium 2.Access No. 2 Ganderma lucidum (Hunan Ganoderma lucidum (Leyss. Ex Fr.) Karst. 1 Number, Hunan Province's non-principal variety of crops certificate of registration numbering XPD010-2013, derive from country's Chinese medicine Management board's subhealth state intervention techniques laboratory) through slant medium activation mycelia, be placed in 120rpm, Cultivating 15 days on 20 DEG C of shaking tables, measurement result is shown in Table 1.
Probiotics fermention is with embodiment 1.Measurement result is shown in Table 1.
Filtering, obtain Ganoderma-fruit ferment product 2, room temperature keeps in Dark Place.
Table 1
Embodiment 3
Fruit and vegerable cultivate: weigh Fructus Vitis viniferae 200g, Radix Dauci Sativae 200g, band leaf Herba Apii graveolentis 100g, Herba Spinaciae 150g, Caulis Zizaniae caduciflorae 300g, white turnip 200g, Chinese cabbage 300g, Fructus Solani melongenae 100g, Radix hemerocalis plicatae 100g, pea seedlings 50g, Rhizoma Solani tuber osi 100g, Fructus Cucumidis sativi 100g, Caulis et Folium Lactucae sativae 80g, Thallus Laminariae (Thallus Eckloniae) 50g, Thallus Porphyrae 50g, Flammulina velutiper (Fr.) Sing 200g, mustard Blue 200g, Brassica campestris L 200g, Semen Phaseoli 300g, homogenate, edible sodium hydroxide solution is adjusted pH to be 6.8, is gone out Bacterium.Access North of Hunan Cordyceps No. 1 (Hunan Province's non-principal variety of crops certificate of registration numbering XPD009-2013, Derive from State Administration of Traditional Chinese Medicine's subhealth state intervention techniques laboratory) through slant medium activation mycelia, 120rpm, 20 DEG C of shaking tables are cultivated 15 days.Measurement result is shown in Table 3.
Probiotics fermention: add 1 ‰ (weight) saccharomyces cerevisiae (CGMCC numbering 2.3888, million grades of viable bacterias Number), 1 ‰ (weight) Lactobacillus buchneri (CGMCC numbering 1.3114, million grades of viable counts), 1 ‰ (weight) Acetobacter (acetobacter Shanghai makes 1.01, brewages a factory, million grades of viable counts purchased from Shanghai), seals, Quiescent culture 7 days under 28 DEG C of environment.Measurement result is shown in Table 2.
Filter off fruit and vegerable slag and undissolved mycelium, obtain clear filtrate, for Cordyceps-fruit-vegerable ferment product 3, Room temperature keeps in Dark Place.
Embodiment 4
Fruit and vegerable are cultivated: weigh Rhizoma Solani tuber osi 1000g, Fructus Kaki 600g, Fructus Ananadis comosi 700g, and homogenate, with edible hydrogen Sodium hydroxide solution adjusts pH to be 6.8, sterilizing.Access Cordyceps militaris (L.) Link. (CGMCC numbering 5.856) through slant culture The mycelia of base activation, 120rpm, 20 DEG C of shaking tables are cultivated 15 days.Measurement result is shown in Table 3.
Probiotics fermention is with embodiment 3.Measurement result is shown in Table 2.
Filter off fruit and vegerable slag and undissolved mycelium, obtain clear filtrate, for Cordyceps-fruit-vegerable ferment product 4, Room temperature keeps in Dark Place.
Table 2

Claims (10)

1. a compositions, is the product cultivated through fruit and vegerable of macro fungi, and fruit and vegerable culture medium therein is by water Fruit and/or vegetable form and without the protein component additionally added.
2. the compositions of claim 1, wherein the macro fungi in product is mycelium form.
3., in the compositions of first any claim, macro fungi therein is breaking cellular wall fungus.
4. in the compositions of first any claim, macro fungi therein selected from Ganoderma, Polyporus, Poria, Cordyceps and/or Cordyceps militaris (L.) Link..
5. a compositions, is that the compositions of one of claim 1-4 carries out probiotics fermention further The product obtained.
6. the method cultivating macro fungi, is with being only made up of fruit and/or vegetable and without additionally adding The fruit and vegerable culture medium of the protein component added is cultivated.
7. ferment manufacture method, is in fruit and vegerable culture medium, and first inoculation macro fungi is cultivated, then connects Planting probiotic bacteria to ferment, described fruit and vegerable culture medium is made up of fruit and/or vegetable and without additionally adding The protein component added.
8. the method for claim 6 or 7, is wherein not related to extract the step of macro fungi active component.
9. the method for one of claim 6-8, macro fungi therein is breaking cellular wall fungus.
10. the method for one of claim 6-8, macro fungi therein selected from Ganoderma, Polyporus, Poria, Cordyceps and/or Cordyceps militaris (L.) Link..
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