CN109090343A - The preparation method of juvenile crab phagostimulant - Google Patents
The preparation method of juvenile crab phagostimulant Download PDFInfo
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Abstract
The invention discloses the preparation methods of juvenile crab phagostimulant, preparation method are as follows: superfine grinding after mixing the various raw materials of juvenile crab phagostimulant according to the weight ratio, obtain mixture, it adds water and is mixed to form paste, it dries after mixing evenly, obtain juvenile crab phagostimulant finished product, the material composition and its parts by weight of juvenile crab phagostimulant are as follows: 10-20 parts of compound amino acid, 4-6 parts of glycine betaine, 5-10 parts of ganoderma active polysaccharide, 6-9 parts of fish guts freeze-dried powder, 3-6 parts of chitosan, 4-8 parts of cordate houttuynia, 1.5-2.5 parts of garlic powder, 2-4 parts of hawthorn, 0.31-0.42 parts of carclazyte.Preparation method of the present invention is simple and convenient to operate, cost is relatively low, and prepared juvenile crab phagostimulant is highly-safe, palatability and digestibility with height, phagostimulating effect is significant, also have the function of disease-prevention health simultaneously, be conducive to the growth and development of juvenile crab, improves culture efficiency.
Description
Technical field
The present invention relates to feed additive fields, more particularly, to the preparation method of juvenile crab phagostimulant.
Technical background
In recent years, the breeding production of aquatic livestock not only may be used so more and more by the way of high density, intensive
To improve yield, while aquaculture cost can be reduced, increase economic benefit.But the main problem that intensive culture production faces is
The control of water quality and aquaculture cost how is reduced as much as possible, appropriate bait throwing in, to reduce residual bait be the key that solve this two large problems
One of means, due to the influence of the factors such as feed or aquatic livestock itself, the feed fed tends not to be ingested and inhaled rapidly
It receives and utilizes.In order to improve aquatic livestock to the utilization efficiency of mixed feed, the nutrition for not only needing to study various aquatic livestocks is needed
It asks, prepares suitable feed, the phagostimulant that aquatic livestock can be promoted to ingest need to be also added in feed, to improve aquatic livestock
Appetite and promote its process of ingesting.With the continuous development of modern aquaculture industry, status of the phagostimulant in mixed feed
It is further obvious, and have become the important topic that aquatic livestock nutritionist extremely pays close attention to and makes great efforts research and development.
Aquatic animal feed phagostimulant is a kind of non-nutritive additive, it is the animals and plants being most fond of according to aquatic livestock
Contained chemical component it is artificial synthesized or extract substance.Phagostimulant is often made of two or more compounds, these chemical combination
Object has synergistic effect to ingesting for aquatic livestock, is stimulated by generations such as smell, the sense of taste and visions to aquatic livestock, keeps it poly-
Collect around feed, quickening, which is ingested, improves feed intake.But have some phagostimulants at present, but because of high production cost, commodity valence
The reasons such as lattice are high, phagostimulating effect is bad, cause its use in aquatic feeds to be restricted.Therefore, it chooses cheap and easy to get
Raw material develop high-efficiency artificial and synthesize phagostimulant, and be allowed to be formed industrialized production there is product for the development of culture fishery
The influence of pole.
The prior art such as Authorization Notice No. is the Chinese invention patent of 103734530 B of CN, discloses a kind of aquatic feeds
Phagostimulant.The phagostimulant is based on total mass fraction 100%, including yeast extract 40~50%, DMPT(Dimethyl-propionic acid thetin)
5~10%, TMAO(trimethylamine oxide) 20~30%, bile salt 5~10%, filler 10~20%.The phagostimulant is by a variety of lists
One phagostimulant and with promote digestion, the bile salt of Hepatoprotective cholagogue function it is compound, to aquatic livestock have good phagostimulating effect
And promote digestion effect.The phagostimulant is added in aquatic feeds, the bile salt contained can effectively facilitate disappearing for feed
Change and absorbs.The aquatic feeds phagostimulant is highly-safe, is free of poisonous and harmful substance, and additive amount is low.But the phagostimulant palatability
Difference, and preparation cost is higher.
Summary of the invention
The purpose of the present invention is to provide one kind, the operation is simple and convenient, the preparation of lower-cost juvenile crab phagostimulant
Method, prepared juvenile crab phagostimulant is highly-safe, and palatability and digestibility with height, phagostimulating effect is significant, feed
Utilization rate is high, while also having the function of disease-prevention health, improves juvenile crab culture efficiency.
The present invention in background technique aiming at the problem that mentioning, the technical solution taken are as follows:
Juvenile crab phagostimulant the preparation method comprises the following steps: superfine grinding after the various raw materials of juvenile crab phagostimulant are mixed according to the weight ratio,
Mixture is obtained, the water for adding 3-4 times of phagostimulant mixture weight is mixed to form paste, dries after mixing evenly at 50-60 DEG C
Dry to get juvenile crab phagostimulant finished product is arrived, prepared juvenile crab phagostimulant material composition, which is arranged in pairs or groups, to be used, and has good food calling effect
Fruit, can also enhance the digestion and absorption function of juvenile crab, while have both the effect of disease-prevention health.
Preferably, the material composition and its parts by weight of juvenile crab phagostimulant are as follows: 10-20 parts of compound amino acid, glycine betaine 4-6
Part, 5-10 parts of ganoderma active polysaccharide, 6-9 parts of fish guts freeze-dried powder, 3-6 parts of chitosan, 4-8 parts of cordate houttuynia, garlic powder 1.5-2.5
Part, 2-4 parts of hawthorn, 0.31-0.42 parts of carclazyte, plurality of raw materials collocation uses in phagostimulant, not only has to juvenile crab and lures well
Effect is eaten, juvenile crab digestion is may additionally facilitate, while also having the function of bactericidal, the disease incidence of juvenile crab can be substantially reduced, wherein
Carclazyte can be improved the dispersibility and mobility of phagostimulant, guarantees that phagostimulant can be dispersed in feed, is conducive to improve
The utilization rate of phagostimulant.
Preferably, the material composition and its parts by weight of compound amino acid are as follows: 10-20 parts of L- glycine, l-Alanine 8-
12 parts, 5-10 parts of glutamic acid, 6-9 parts of histidine, compound amino acid can not only improve the palatability of feed, and juvenile crab is promoted to ingest,
Increase food ration, promote growth, improve the utilization rate of feed, reduces secondary pollution of the defective material to water body environment, additionally it is possible to stimulate
The secretion of digestive ferment improves enzymatic activity, to enhance the digestion and absorption function of juvenile crab, while also providing for the growth of juvenile crab required
Amino acid nutrient.
Preferably, the extracting method of ganoderma active polysaccharide are as follows: select the ganoderma lucidum dried, smash it through 60-80 mesh, obtain
Glossy ganoderma powder, the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester that 6-8 times of glossy ganoderma powder weight is added stir evenly, first
Glossy ganoderma powder weight 0.5-0.9% cellulase, 40-45 DEG C of controlled enzymatic hydrolysis temperature is added, pH value 4.3-5.0 digests 20-30min,
After add the alkali protease of glossy ganoderma powder weight 0.2-0.6%, 45-50 DEG C of controlled enzymatic hydrolysis temperature, pH value 6.8-7.5, enzyme
Solve 20-30min, after enzymatic hydrolysis at 100-105 DEG C enzyme deactivation 5-10min, obtain enzymolysis liquid, obtained enzymolysis liquid be placed in vacuum concentration
It is concentrated in device, decompression low temperature is concentrated into enzymolysis liquid to the 1/3 of original volume, obtains ganoderma lucidum crude polysaccharide concentrate, concentrate presses 1:4-5 ratio
Example is added 90-95% ethanol precipitation and stands 10-20h, is centrifuged 10-20min in the case where revolving speed is 4000-4500rpm, collects precipitating simultaneously
It is cleaned 2-3 times repeatedly with ethyl alcohol, freeze-drying obtains ganoderma active polysaccharide, and extracted ganoderma active polysaccharide is best in quality,
Active skull cap components are high, have various health-care and pharmacological action, especially adjusting immune function and inhibit the effect of tumour,
Bioactivity is high, has good phagostimulating effect to juvenile crab.
Further preferably, the L-TARTARIC ACID diformazan of the sorbierite in deionized water containing 0.2-0.4% and 0.03-0.05%
Ester handles glossy ganoderma powder using the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester, on the one hand can make fibre with swollen spirit slag
Tieing up plain enzyme and alkali protease can rapidly and efficiently contact with fat, the cellulose in ganoderma lucidum, and then accelerate enzymatic hydrolysis rate, make born of the same parents
Interior polysaccharide is more discharged, and is conducive to improve polysaccharide extract rate；Another aspect sorbierite and L-TARTARIC ACID dimethyl ester are arranged in pairs or groups
It uses, plays synergistic effect, can effectively destroy intercellular layer structure in cell wall, accelerate the rupture of cell wall, further increase and mention
Efficiency is taken, intracellular effective polysaccharide is made to be released diffusion, so that the ganoderma lucidum polysaccharide bioactivity extracted is high, and then improves it
Phagostimulating effect.
Preferably, fish guts freeze-dried powder the preparation method comprises the following steps: cleaning fish guts to no color, be sent into sonicator
It handles through ultrasonication, after making its granularity < 3-4 μm, is placed in enzymatic vessel, complex enzyme hydrolysis liquid is added, control solid-liquid ratio is 1:4-
5(g:mL), at 35-40 DEG C, 3-4h is digested, every 30-40min stirring is primary, and pH is controlled between 5.3-7.5, enzymatic hydrolysis end
Afterwards, enzymolysis liquid is warming up to 85-100 DEG C, enzyme deactivation 20-30min, freeze-drying obtains fish guts freeze-dried powder, obtained fish
Internal organ freeze-dried powder nutritive value is high, and performance is excellent, is rich in a variety of amino acid, polypeptide molecular weight is low to be easily absorbed and utilized by juvenile crab, favorably
It is at low cost in promoting juvenile crab fast-growth, while using fish guts as phagostimulant working process, and avoid it and be taken as waste material
Accumulation, and then economic loss and environmental pollution caused by rotten erosion.
Further preferably, the raw material and its parts by weight in complex enzyme hydrolysis liquid are as follows: 10-15 parts of trypsase, papain
5-10 parts, 3-5 parts of mycosamine, 1-2 parts of aurantiamarin, mycosamine and aurantiamarin collocation use, performance synergistic function, and one
The activity of complex enzyme can be improved in aspect, promotes the combination between enzyme and fish guts albumen, and then improves enzymatic hydrolysis rate；Another party
Face makes the spiral space structure of fish guts albumen untwist by influencing the active group on hydroxyproline, and molecular structure becomes
Loosely, enzyme binding site increases, and is conducive to the combination of enzyme-to-substrate in enzymolysis process, improves the content of peptides in enzymolysis liquid, and
The molecular weight of gained polypeptide is lower to be easily absorbed and utilized by juvenile crab, may advantageously facilitate juvenile crab fast-growth.
Compared with the prior art, the advantages of the present invention are as follows: 1) the preparation method simple possible of of the present invention juvenile crab phagostimulant,
Raw material acquisition difficulty is small, at low cost；2) juvenile crab phagostimulant of the present invention is composite feeding promoting agent, is got well than single phagostimulant effect, single
One phagostimulant only may form single stimulation to the smell of juvenile crab, vision and the sense of taste, and composite feeding promoting agent can be formed it is comprehensive
Food calling stimulation, this stimulation is stronger, preferably juvenile crab can be lured to ingest；3) juvenile crab phagostimulant of the present invention is highly-safe, tool
There is the palatability of height, phagostimulating effect is significant, is greatly improved the utilization rate of feed, the secretion for the enzyme that can stimulate digestion, and improves
Enzymatic activity to enhance the digestion and absorption function of juvenile crab, while also having the function of disease-prevention health, is conducive to the growth hair of juvenile crab
It educates, improves culture efficiency.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
Juvenile crab phagostimulant the preparation method comprises the following steps: superfine grinding after the various raw materials of juvenile crab phagostimulant are mixed according to the weight ratio,
Food calling agent composition is obtained, 3 times of phagostimulant mixture weight of water is added and is mixed to form paste, is dried after mixing evenly at 50 DEG C
Dry to get juvenile crab phagostimulant finished product is arrived, prepared juvenile crab phagostimulant material composition, which is arranged in pairs or groups, to be used, and has good food calling effect
Fruit, can also enhance the digestion and absorption function of juvenile crab, while have both the effect of disease-prevention health.
The material composition and its parts by weight of above-mentioned juvenile crab phagostimulant are as follows: 10 parts of compound amino acid, 6 parts of glycine betaine, ganoderma lucidum are living
Property 5 parts of polysaccharide, 7 parts of fish guts freeze-dried powder, 3 parts of chitosan, 4 parts of cordate houttuynia, 1.5 parts of garlic powder, 2 parts of hawthorn, 0.35 part of carclazyte,
Plurality of raw materials collocation uses in phagostimulant, not only has good phagostimulating effect to juvenile crab, may additionally facilitate juvenile crab digestion, while
Have the function of bactericidal, the disease incidence of juvenile crab can be substantially reduced, wherein carclazyte can be improved the dispersibility and stream of phagostimulant
Dynamic property guarantees that phagostimulant can be dispersed in feed, is conducive to the utilization rate for improving phagostimulant.
The material composition and its parts by weight of above-mentioned compound amino acid are as follows: 10 parts of L- glycine, 8 parts of l-Alanine, glutamic acid 6
Part, 8 parts of histidine, compound amino acid can not only improve the palatability of feed, and juvenile crab is promoted to ingest, and increase food ration, promote life
It is long, the utilization rate of feed is improved, secondary pollution of the defective material to water body environment is reduced, additionally it is possible to which the secretion for the enzyme that stimulates digestion improves
Enzymatic activity to enhance the digestion and absorption function of juvenile crab, while also providing required amino acid nutrient for the growth of juvenile crab.
The extracting method of above-mentioned ganoderma active polysaccharide are as follows: the ganoderma lucidum dried is selected, 60 meshes is smashed it through, obtains glossy ganoderma powder,
6 times of glossy ganoderma powder weight of the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester is added to stir evenly, is firstly added ganoderma lucidum
0.5% cellulase of powder weight, 40 DEG C of controlled enzymatic hydrolysis temperature, pH value 4.3-5.0, digest 20min, after add glossy ganoderma powder
The alkali protease of weight 0.2%, controlled enzymatic hydrolysis temperature 45 C, pH value 6.8-7.5 digest 30min, go out at 100 DEG C after enzymatic hydrolysis
Enzyme 5min, obtains enzymolysis liquid, and obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, and decompression low temperature is concentrated into enzymolysis liquid to substance
Long-pending 1/3 obtains ganoderma lucidum crude polysaccharide concentrate, and concentrate is added 90% ethanol precipitation in 1:4 ratio and stands 10h, is in revolving speed
It is centrifuged 10min under 4500rpm, collects and precipitates and cleaned repeatedly with ethyl alcohol 2 times, freeze-drying obtains ganoderma active polysaccharide, institute
The ganoderma active polysaccharide of extraction is best in quality, and active skull cap components are high, has various health-care and pharmacological action, especially adjusts
It saves immune function and inhibits the effect of tumour, bioactivity is high, has good phagostimulating effect to juvenile crab.
Containing 0.2% sorbierite and 0.03% L-TARTARIC ACID dimethyl ester in above-mentioned deionized water, using containing sorbierite
Glossy ganoderma powder is handled with the deionized water of L-TARTARIC ACID dimethyl ester, on the one hand can make cellulase and basic protein with swollen spirit slag
Enzyme can be contacted rapidly and efficiently with fat, the cellulose in ganoderma lucidum, and then accelerate enzymatic hydrolysis rate, obtain intracellular polyse more
Release is conducive to improve polysaccharide extract rate；Another aspect sorbierite and the collocation of L-TARTARIC ACID dimethyl ester use, and play collaboration and make
With can effectively destroy intercellular layer structure in cell wall, accelerate the rupture of cell wall, further increase extraction efficiency, make intracellular
Effective polysaccharide be released diffusion so that the ganoderma lucidum polysaccharide bioactivity extracted is high, and then improve its phagostimulating effect.
Above-mentioned fish guts freeze-dried powder the preparation method comprises the following steps: cleaning fish guts to no color, be sent into sonicator through super
Sound break process is placed in enzymatic vessel after making 3 μm of its granularity <, and complex enzyme hydrolysis liquid is added, and control solid-liquid ratio is 1:4(g:mL),
3h is digested at 35 DEG C, every 30min stirring is primary, and pH is controlled between 5.3-7.5, and after enzymatic hydrolysis terminates, enzymolysis liquid is heated up
To 85 DEG C, enzyme deactivation 20min, freeze-drying obtains fish guts freeze-dried powder, and obtained fish guts freeze-dried powder nutritive value is high, property
Can be excellent, a variety of amino acid are rich in, polypeptide molecular weight is low to be easily absorbed and utilized by juvenile crab, may advantageously facilitate juvenile crab fast-growth, simultaneously
It is at low cost using fish guts as phagostimulant working process, and avoid it and be taken as waste from stacking, so it is rotten it is rotten to the corn caused by
Economic loss and environmental pollution.
Raw material and its parts by weight in above-mentioned complex enzyme hydrolysis liquid are as follows: 10 parts of trypsase, 5 parts of papain, trehalose
4 parts of amine, 1 part of aurantiamarin, mycosamine and aurantiamarin collocation use, and play synergistic function, on the one hand can be improved compound
The activity of enzyme promotes the combination between enzyme and fish guts albumen, and then improves enzymatic hydrolysis rate；On the other hand by influencing hydroxyl dried meat ammonia
Active group on acid makes the spiral space structure of fish guts albumen untwist, and molecular structure becomes loosely, enzyme binding site
Increase, be conducive to the combination of enzyme-to-substrate in enzymolysis process, improves the content of peptides in enzymolysis liquid, and the molecular weight of gained polypeptide
It is lower to be easily absorbed and utilized by juvenile crab, it may advantageously facilitate juvenile crab fast-growth.
Embodiment 2:
Juvenile crab phagostimulant the preparation method comprises the following steps: superfine grinding after the various raw materials of juvenile crab phagostimulant are mixed according to the weight ratio,
Food calling agent composition is obtained, 4 times of phagostimulant mixture weight of water is added and is mixed to form paste, is dried after mixing evenly at 55 DEG C
It does to get juvenile crab phagostimulant is arrived.
The material composition and its parts by weight of above-mentioned juvenile crab phagostimulant are as follows: 20 parts of compound amino acid, 4 parts of glycine betaine, ganoderma lucidum are living
Property 8 parts of polysaccharide, 7 parts of fish guts freeze-dried powder, 6 parts of chitosan, 4 parts of cordate houttuynia, 2.0 parts of garlic powder, 4 parts of hawthorn, 0.4 part of carclazyte.
The material composition and its parts by weight of above-mentioned compound amino acid are as follows: 10 parts of L- glycine, 8 parts of l-Alanine, glutamic acid 5
Part, 9 parts of histidine.
The extracting method of above-mentioned ganoderma active polysaccharide are as follows: the ganoderma lucidum dried is selected, 80 meshes is smashed it through, obtains glossy ganoderma powder,
7 times of glossy ganoderma powder weight of the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester is added to stir evenly, glossy ganoderma powder is first added
0.6% cellulase of weight, controlled enzymatic hydrolysis temperature 45 C, pH value 4.3-5.0 digest 30min, and glossy ganoderma powder weight is added later
0.4% alkali protease, controlled enzymatic hydrolysis temperature 45 C, pH value 6.8-7.5 digest 30min, the enzyme deactivation at 100 DEG C after enzymatic hydrolysis
10min obtains enzymolysis liquid, and obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, and decompression low temperature is concentrated into enzymolysis liquid to substance
Long-pending 1/3 obtains ganoderma lucidum crude polysaccharide concentrate, and concentrate is added 95% ethanol precipitation in 1:5 ratio and stands 15h, is in revolving speed
It is centrifuged 10min under 4500rpm, collects and precipitates and cleaned repeatedly with ethyl alcohol 3 times, freeze-drying obtains ganoderma active polysaccharide.
Contain 0.3% sorbierite and 0.05% L-TARTARIC ACID dimethyl ester in above-mentioned deionized water.
Above-mentioned fish guts freeze-dried powder the preparation method comprises the following steps: cleaning fish guts to no color, be sent into sonicator through super
Sound break process is placed in enzymatic vessel after making 4 μm of its granularity <, and complex enzyme hydrolysis liquid is added, and control solid-liquid ratio is 1:4(g:mL),
4h is digested at 40 DEG C, every 30min stirring is primary, and pH is controlled between 5.3-7.5, and after enzymatic hydrolysis terminates, enzymolysis liquid is heated up
To 100 DEG C, enzyme deactivation 20min, freeze-drying obtains fish guts freeze-dried powder.
Raw material and its parts by weight in above-mentioned complex enzyme hydrolysis liquid are as follows: 15 parts of trypsase, 7 parts of papain, trehalose
5 parts of amine, 2 parts of aurantiamarin.
Embodiment 3:
Juvenile crab phagostimulant the preparation method comprises the following steps: superfine grinding after the various raw materials of juvenile crab phagostimulant are mixed according to the weight ratio,
Food calling agent composition is obtained, 4 times of phagostimulant mixture weight of water is added and is mixed to form paste, is dried after mixing evenly at 60 DEG C
It does to get juvenile crab phagostimulant finished product is arrived.
The material composition and its parts by weight of above-mentioned juvenile crab phagostimulant are as follows: 15 parts of compound amino acid, 6 parts of glycine betaine, ganoderma lucidum are living
Property 7 parts of polysaccharide, 8 parts of fish guts freeze-dried powder, 4 parts of chitosan, 6 parts of cordate houttuynia, 1.5 parts of garlic powder, 3 parts of hawthorn, 0.33 part of carclazyte.
The material composition and its parts by weight of above-mentioned compound amino acid are as follows: 10 parts of L- glycine, 12 parts of l-Alanine, glutamic acid
9 parts, 7 parts of histidine.
The extracting method of above-mentioned ganoderma active polysaccharide are as follows: the ganoderma lucidum dried is selected, 60 meshes is smashed it through, obtains glossy ganoderma powder,
7 times of glossy ganoderma powder weight of the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester is added to stir evenly, glossy ganoderma powder is first added
0.8% cellulase of weight, controlled enzymatic hydrolysis temperature 45 C, pH value 4.3-5.0 digest 20min, and glossy ganoderma powder weight is added later
0.2% alkali protease, controlled enzymatic hydrolysis temperature 50 C, pH value 6.8-7.5 digest 20min, the enzyme deactivation at 105 DEG C after enzymatic hydrolysis
5min obtains enzymolysis liquid, and obtained enzymolysis liquid is placed in vacuum concentrator and is concentrated, and decompression low temperature is concentrated into enzymolysis liquid to original volume
1/3, obtain ganoderma lucidum crude polysaccharide concentrate, concentrate is added 90% ethanol precipitation in 1:5 ratio and stands 15h, is in revolving speed
It is centrifuged 15min under 4000rpm, collects and precipitates and cleaned repeatedly with ethyl alcohol 2 times, freeze-drying obtains ganoderma active polysaccharide.
Contain 0.2% sorbierite and 0.04% L-TARTARIC ACID dimethyl ester in above-mentioned deionized water.
Above-mentioned fish guts freeze-dried powder the preparation method comprises the following steps: cleaning fish guts to no color, be sent into sonicator through super
Sound break process is placed in enzymatic vessel after making 4 μm of its granularity <, and complex enzyme hydrolysis liquid is added, and control solid-liquid ratio is 1:4(g:mL),
4h is digested at 40 DEG C, every 40min stirring is primary, and pH is controlled between 5.3-7.5, and after enzymatic hydrolysis terminates, enzymolysis liquid is heated up
To 90 DEG C, enzyme deactivation 20min, freeze-drying obtains fish guts freeze-dried powder.
Raw material and its parts by weight in above-mentioned complex enzyme hydrolysis liquid are as follows: 12 parts of trypsase, 8 parts of papain, trehalose
3 parts of amine, 2 parts of aurantiamarin.
Embodiment 4:
The addition of juvenile crab phagostimulant prepared by embodiment 1, embodiment 2, embodiment 3 (additive amount is the 0.6% of basic feed) is arrived
It is used as experimental group in basal feed, feeds the crab that specification is 2g respectively as a control group with the basal feed for not adding phagostimulant
Seedling, nursing time 30d, concrete outcome such as table 1
| Juvenile crab average weight gain multiple | Average survival (%) | |
| Embodiment 1 | 6.35 | 79.3 |
| Embodiment 2 | 6.79 | 82.8 |
| Embodiment 3 | 6.12 | 80.6 |
| Control group | 5.09 | 66.4 |
As shown in Table 1, compared with the control group, the average weight gain multiple and average survival of the juvenile crab of experimental group are higher, it is seen then that
The phagostimulating effect of juvenile crab phagostimulant of the present invention is good, and the utilization rate of feed is high, has greatly facilitated the growth and development of juvenile crab.
Routine operation in operation of the present invention step is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (7)
1. the preparation method of juvenile crab phagostimulant, it is characterised in that: described specific the preparation method comprises the following steps: by the various originals of juvenile crab phagostimulant
Material mixing, grinding, add water to be tuned into paste, are dried after mixing evenly to get juvenile crab phagostimulant, the juvenile crab phagostimulant raw material is arrived
Ingredient and its parts by weight are as follows: 10-20 parts of compound amino acid, 4-6 parts of glycine betaine, 5-10 parts of ganoderma active polysaccharide, fish guts freeze-drying
6-9 parts of powder, 3-6 parts of chitosan, 4-8 parts of cordate houttuynia, 1.5-2.5 parts of garlic powder, 2-4 parts of hawthorn, 0.31-0.42 parts of carclazyte.
2. the preparation method of juvenile crab phagostimulant according to claim 1, it is characterised in that: the ganoderma active polysaccharide mentions
Take method are as follows: glossy ganoderma powder is stirred evenly with 6-8 times of its weight of the deionized water containing sorbierite and L-TARTARIC ACID dimethyl ester,
Cellulase, 40-45 DEG C of controlled enzymatic hydrolysis temperature is first added, pH value 4.3-5.0 digests 20-30min, basic protein is added later
Enzyme, 45-50 DEG C of controlled enzymatic hydrolysis temperature, pH value 6.8-7.5 digest 20-30min, after enzymatic hydrolysis at 100-105 DEG C enzyme deactivation 5-
10min obtains enzymolysis liquid, and obtained enzymolysis liquid decompression low temperature is concentrated into enzymolysis liquid to the 1/3 of original volume, it is dense to obtain ganoderma lucidum crude polysaccharide
Contracting liquid, concentrate are added 90-95% ethanol precipitation in 1:4-5 ratio and stand 10-20h, and centrifugation, washing, freeze-drying obtain spirit
Sesame active polysaccharide.
3. the preparation method of juvenile crab phagostimulant according to claim 2, it is characterised in that: contain in the deionized water
The sorbierite of 0.2-0.4% and the L-TARTARIC ACID dimethyl ester of 0.03-0.05%.
4. the preparation method of juvenile crab phagostimulant according to claim 2, it is characterised in that: the additive amount of the cellulase
For glossy ganoderma powder weight 0.5-0.9%, the additive amount of the alkali protease is glossy ganoderma powder weight 0.2-0.6%.
5. the preparation method of juvenile crab phagostimulant according to claim 1, it is characterised in that: the raw material of the compound amino acid
Ingredient and its parts by weight are as follows: 10-20 parts of L- glycine, 8-12 parts of l-Alanine, 5-10 parts of glutamic acid, 6-9 parts of histidine.
6. the preparation method of juvenile crab phagostimulant according to claim 1, it is characterised in that: the system of the fish guts freeze-dried powder
Preparation Method are as follows: fish guts are cleaned to no color, ultrasonication, after making its granularity < 3-4 μm, are placed in enzymatic vessel, are added multiple
Enzymolysis liquid is closed, control solid-liquid ratio is 1:4-5(g:mL), at 35-40 DEG C, 3-4h is digested, every 30-40min stirring is primary, pH control
Between 5.3-7.5, after enzymatic hydrolysis terminates, enzymolysis liquid is warming up to 85-100 DEG C, enzyme deactivation 20-30min, freeze-drying obtains
Fish guts freeze-dried powder.
7. the preparation method of juvenile crab phagostimulant according to claim 6, it is characterised in that: the original in the complex enzyme hydrolysis liquid
Material and its parts by weight are as follows: 10-15 parts of trypsase, 5-10 parts of papain, 3-5 parts of mycosamine, 1-2 parts of aurantiamarin.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112425685A (en) * | 2020-11-18 | 2021-03-02 | 广西科技大学 | Preparation method of phagostimulant for artificial culture of escargots |
| GB2586656A (en) * | 2019-09-02 | 2021-03-03 | The Univ Of Hull | Semiochemical delivery matrices |
-
2018
- 2018-09-11 CN CN201811057246.7A patent/CN109090343A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2586656A (en) * | 2019-09-02 | 2021-03-03 | The Univ Of Hull | Semiochemical delivery matrices |
| CN112425685A (en) * | 2020-11-18 | 2021-03-02 | 广西科技大学 | Preparation method of phagostimulant for artificial culture of escargots |
| CN112425685B (en) * | 2020-11-18 | 2022-11-01 | 广西科技大学 | Preparation method of phagostimulant for artificial culture of escargots |
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