CN109077957B - Traditional Chinese medicine composition fermentation liquor with anti-pollution effect for cosmetics and preparation and application thereof - Google Patents
Traditional Chinese medicine composition fermentation liquor with anti-pollution effect for cosmetics and preparation and application thereof Download PDFInfo
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The invention discloses a traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics, and preparation and application thereof, and belongs to the field of cosmetics. The traditional Chinese medicine composition fermentation liquor for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 10-40 parts of sophora flower bud, 5-30 parts of ginkgo leaf, 5-30 parts of wild chrysanthemum flower, 5-30 parts of cocklebur fruit and 5-30 parts of liquorice. The fermentation liquor of the traditional Chinese medicine composition for cosmetics has the characteristic of natural safety. The invention takes the monarch, minister, assistant and guide theory of traditional Chinese medicine as guidance to carry out prescription, and obtains the anti-pollution purpose from the aspects of comprehensive efficacies of clearing away heat and toxic material, removing free radicals, resisting allergy, resisting ultraviolet rays, inhibiting bacteria, promoting skin healing and the like through the synergistic effect of a plurality of traditional Chinese medicines. The traditional Chinese medicine composition fermentation liquor disclosed by the invention is applied to cosmetics prepared from the cosmetics through a scientific formula, and has a good effect of resisting the damage of air pollution to the skin.
Description
Technical Field
The invention relates to a traditional Chinese medicine composition fermentation liquor, in particular to a traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics, and preparation and application thereof, and belongs to the field of cosmetics.
Background
In recent years, the number of skin patients has increased dramatically, and the reasons for this are many, among which air pollution is an important part, and the following skin problems are easily caused by air pollution: one is causing skin allergy: a large amount of dispersed floating matters in the air are allergic to cause blockage of skin pores and allergic symptoms, so that people feel pruritus, develop erythema and whelk, and even trigger urticaria and eczema. Secondly, the skin is aged: pollutants in the air, such as haze and the like, can cause the skin to generate an excessive amount of extremely unstable free radicals, and the free radicals can damage cell membranes and DNA (deoxyribonucleic acid) of human body self health, so that the aging of the body is accelerated, and the skin is aged, such as dull skin, pigmentation, easy relaxation, fine lines or wrinkles, dry skin and even skin cancerization. Whether it causes skin infection easily: haze causes excessive particles in the air, and the degree of light transmission is reduced, so that ultraviolet rays reaching the vicinity of the ground are weakened. Thus various viruses, bacteria and fungi floating in the air near the ground become less susceptible to being killed by ultraviolet rays. For people with poor physique, skin allergy and damaged skin, the microorganisms reach the skin along with dust particles in the air and are adhered to the surface of the skin, so that skin infection is easily caused, such as folliculitis, sweat stain, herpes simplex and the like, and people with skin rash are aggravated.
Therefore, to resist the damage of external pollution to skin, the treatment should be done with the aspects of expelling toxin, resisting allergy, scavenging free radicals, isolating ultraviolet rays, inhibiting bacteria, cleaning, etc.
Disclosure of Invention
In order to overcome the defects and shortcomings in the prior art and combine with the theoretical basis of traditional Chinese medicine, the invention mainly aims to provide the traditional Chinese medicine composition fermentation liquor for cosmetics with an anti-pollution effect. The fermentation liquid of the Chinese medicinal composition for cosmetics has the advantages of natural property and safety.
The invention also aims to provide a preparation method of the fermentation liquor of the traditional Chinese medicine composition for cosmetics with the anti-pollution effect.
The invention further aims to provide application of the fermentation liquor of the traditional Chinese medicine composition for cosmetics with an anti-pollution effect.
The purpose of the invention is realized by the following technical scheme:
a traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 10-40 parts of sophora flower bud, 5-30 parts of ginkgo leaf, 5-30 parts of wild chrysanthemum flower, 5-30 parts of cocklebur fruit and 5-30 parts of liquorice.
Preferably, the anti-pollution traditional Chinese medicine composition fermentation liquor for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 25-30 parts of sophora flower bud, 15-20 parts of ginkgo leaf, 17-20 parts of wild chrysanthemum flower, 18-20 parts of cocklebur fruit and 10-20 parts of liquorice.
Preferably, the traditional Chinese medicine composition fermentation liquor (I) with the anti-pollution effect for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 28 parts of sophora flower bud, 20 parts of ginkgo leaf, 17 parts of wild chrysanthemum flower, 18 parts of cocklebur fruit and 17 parts of liquorice.
Preferably, the cosmetic traditional Chinese medicine composition fermentation liquor (II) with the anti-pollution effect is prepared from the following traditional Chinese medicine components in parts by weight: 30 parts of sophora flower bud, 20 parts of ginkgo leaf, 20 parts of wild chrysanthemum flower, 20 parts of cocklebur fruit and 10 parts of liquorice.
Preferably, the cosmetic traditional Chinese medicine composition fermentation liquor (III) with the anti-pollution effect is prepared from the following traditional Chinese medicine components in parts by weight: 25 parts of sophora flower bud, 15 parts of ginkgo leaf, 20 parts of wild chrysanthemum flower, 20 parts of cocklebur fruit and 20 parts of liquorice.
Preferably, the composite bacteria used for fermentation are lactobacillus plantarum and saccharomyces cerevisiae;
preferably, the lactobacillus plantarum and the saccharomyces cerevisiae in the composite bacteria are mixed according to the proportion (1-5): 1 composition, the concentration of the compound bacterial liquid is 106～108cfu/mL。
The second purpose of the invention is to provide a preparation method of the fermentation liquor of the traditional Chinese medicine composition for cosmetics with the anti-pollution effect.
In order to achieve the purpose, the invention adopts a fermentation extraction method, namely, the traditional Chinese medicine composition is fermented, and then is filtered, sterilized and centrifugally separated to obtain the traditional Chinese medicine composition fermentation liquor. The method specifically comprises the following steps:
firstly, adding composite bacteria in the form of bacteria liquid, wherein the composite bacteria liquid is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion (1-5): 1 composition, the concentration of the compound bacterial liquid is 106～108cfu/mL；
Preparing materials: removing impurities from the traditional Chinese medicine raw materials, cleaning, drying, crushing to 80-200 meshes, and mixing uniformly to obtain a traditional Chinese medicine composition; the preparation concentration is 106～108Adjusting the pH value of cfu/mL composite bacterial liquid to 6.5-7.5;
③ fermentation: mixing the compound bacterial liquid, the traditional Chinese medicine composition and water according to a ratio, and fermenting for 36-48 hours at the temperature of 28-42 ℃ and at the speed of 50-300 r/min to obtain fermentation liquor;
purifying: filtering the obtained fermentation liquor, and autoclaving the filtrate at 105-120 ℃ for 20-35 min to inactivate the strains; centrifuging the sterilized fermentation filtrate for 20-35 min under the conditions of 3000-8000 r/min and a centrifugation radius of 5-20 cm, discarding the precipitate, collecting the supernatant, concentrating by using a membrane, adsorbing the concentrated solution by using macroporous resin, eluting for multiple times by using ethanol with a volume fraction of 10-95%, collecting the eluent, concentrating by using the membrane to obtain a concentrated solution with the same weight as the traditional Chinese medicine composition, adding butanediol with the same weight as the concentrated solution, dissolving, and filtering to obtain the traditional Chinese medicine composition fermentation liquor for cosmetics with the anti-pollution effect.
Preferably, in the step (i), the composite bacterial liquid is prepared by mixing lactobacillus plantarum and saccharomyces cerevisiae according to a proportion of 3: 1, preparing a composition;
preferably, in the third step, fermentation is carried out for 36-48 hours at the temperature of 28-35 ℃ and at the speed of 100r/min, so as to obtain fermentation liquor;
the dosage ratio of the compound bacterial liquid, the traditional Chinese medicine composition and water is 20-60 mL: 20 g-60 g: 200-600 g.
Preferably, the dosage ratio of the compound bacterial liquid, the traditional Chinese medicine composition and water is 25-40 mL: 30 g-45 g: 300-400 g.
Preferably, the traditional Chinese medicine composition fermentation liquor (I) is prepared by mixing the compound bacterial liquid, the traditional Chinese medicine composition and water in a dosage ratio of 30 mL: 40 g: 300 g.
Preferably, the amount ratio of the compound bacterial liquid to the traditional Chinese medicine composition in the traditional Chinese medicine composition fermentation liquid (II) to water is 40 mL: 45 g: 400 g.
Preferably, the amount ratio of the traditional Chinese medicine composition fermentation liquor (III) to the composite bacterial liquid to the traditional Chinese medicine composition to water is 25 mL: 30g of: 300 g.
Preferably, the centrifugal radius in the step (iv) is 8-12 cm.
Preferably, in the step (iv), the filtrate is autoclaved at the temperature of 110-120 ℃ for 30min to inactivate the strains; and centrifuging the sterilized fermentation filtrate for 30min under the conditions of 6000-8000 r/min and the centrifugal radius of 9cm, removing the precipitate, and collecting the supernatant.
The third purpose of the invention is to provide the application of the fermentation liquor of the traditional Chinese medicine composition for cosmetics with anti-pollution effect in cosmetics.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme: the application of the anti-pollution traditional Chinese medicine composition fermentation liquor for cosmetics in preparing cosmetics comprises the anti-pollution traditional Chinese medicine composition fermentation liquor for cosmetics, and the addition amount of the anti-pollution traditional Chinese medicine composition fermentation liquor is 0.1-15% by mass.
The invention also provides a cosmetic which contains the traditional Chinese medicine composition fermentation liquor for cosmetics with the anti-pollution effect.
Preferably, the cosmetic contains 0.1-15% of the traditional Chinese medicine composition fermentation liquor for the cosmetic with the anti-pollution effect by mass fraction.
The cosmetic is as follows: aqueous preparations, emulsions, creams, gels, powders, powder-forming products, facial masks, sprays, essences, and lotions.
Specifically, the invention provides an anti-pollution mask which comprises the following components in parts by mass:
| components | Mass fraction/% |
| The traditional Chinese medicine composition fermentation liquor | 5 |
| Glycerol | 6.00 |
| 1, 3-propanediol | 4.00 |
| Hyaluronic acid sodium salt | 0.10 |
| Xanthan gum | 0.10 |
| Hydroxyethyl cellulose | 0.10 |
| Glycyrrhizic acid dipotassium salt | 0.50 |
| Aloe extract | 1.00 |
| 1, 2-hexanediol | 0.80 |
| P-hydroxyacetophenone | 0.50 |
| Deionized water | Balance of |
Specifically, the invention provides an anti-pollution skin cream which comprises the following components in parts by mass:
| composition (I) | Mass fraction (%) |
| Arachidyl alcohol glucoside | 1.5 |
| PEG-100 stearate | 1.2 |
| Glycerol stearate | 1 |
| Cetearyl glucoside | 0.8 |
| Shea butter | 0.5 |
| Potentilla chinensis seed oil | 3 |
| Hydrogenated polyisobutenes | 3 |
| Cyclopolydimethylsiloxane | 4 |
| Polydimethylsiloxane | 2 |
| Hydroxy phenyl methyl ester | 0.2 |
| Propyl hydroxybenzoate | 0.08 |
| EDTA‐2Na | 0.02 |
| Glycerol | 5 |
| 1, 3-butanediol | 3 |
| Hyaluronic acid sodium salt | 0.05 |
| Carbomer (CA) | 0.2 |
| Phenoxyethanol | 0.3 |
| Aminomethyl propanol | 0.12 |
| Essence | 0.1 |
| The traditional Chinese medicine composition fermentation liquor | 5 |
| Deionized water | Balance of |
The mechanism of the invention is as follows:
the beauty theory of traditional Chinese medicine holds that good skin needs external decoration, and more importantly, the skin is healthy from inside to outside. Although the reasons for forming the skin problems are various, the strong skin can resist external stimulation to a certain extent, so the anti-pollution in the traditional Chinese medicine is a comprehensive conditioning process, and the anti-pollution capability of the traditional Chinese medicine is achieved by enhancing the immunity of the skin.
The Chinese medicinal composition is prepared by taking the monarch, minister, assistant and guide theory of the traditional Chinese medicine as guidance, and the pagodatree flower bud is taken as a monarch medicament, has special effects of expelling toxin and accelerating metabolism, can accelerate skin metabolism and expelling toxin, and has very good effects of expelling toxin, beautifying, resisting free radicals, resisting ultraviolet rays and repairing tissue regeneration; the ginkgo leaves and the wild chrysanthemum flowers are used as ministerial drugs together, the ginkgo leaves have very strong effects of clearing free radicals, resisting aging, enhancing immunity and good anti-allergy, and the wild chrysanthemum flowers have broad-spectrum antibacterial effect and very strong inhibition effect on acne bacilli and the like; the fructus xanthil is an adjuvant drug and has very strong efficacies of relieving exterior syndrome, dispelling cold, expelling wind and dampness and relieving itching; the licorice root is a guiding drug, has the effects of clearing away heat and toxic materials, resisting inflammation, resisting free radicals and the like, and has the effect of harmonizing the medicines. The medicines are used together to strengthen the body resistance, strengthen the vitality, nourish and condition so as to strengthen the skin as the anti-pollution root; the anti-pollution targets are cleaning, toxin expelling, anti-inflammation, sterilization, relief and anti-allergy. The traditional Chinese medicine composition fermentation liquor disclosed by the invention has very good comprehensive effects of clearing away heat and toxic materials, removing free radicals, resisting allergy, resisting ultraviolet rays, inhibiting bacteria, promoting skin healing and the like, and has an obvious effect of resisting air pollution when being added into cosmetics.
Especially, the fermentation extraction method can change the molecular structure of the raw material components, improve the efficacy of the raw material, reduce the toxicity and the irritation, optimize the color, the taste and other advantages, is an ideal new traditional Chinese medicine extraction process, and is beneficial to popularization and application.
Compared with the prior art, the invention has the following advantages and effects:
the invention takes the monarch, minister, assistant and guide theory of traditional Chinese medicine as guidance to carry out prescription, and obtains the anti-pollution purpose from the aspects of comprehensive efficacies of clearing away heat and toxic material, removing free radicals, resisting allergy, resisting ultraviolet rays, inhibiting bacteria, promoting skin healing and the like through the synergistic effect of a plurality of traditional Chinese medicines. The traditional Chinese medicine composition fermentation liquor disclosed by the invention is applied to cosmetics prepared from the cosmetics through a scientific formula, and has a good effect of resisting the damage of air pollution to the skin.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
The test methods in the following examples, in which specific experimental conditions are not specified, are generally performed according to conventional experimental conditions or according to the experimental conditions recommended by the manufacturer.
Lactobacillus plantarum (Lactobacillus plantarum) used in the examples was obtained from changzhou probiotic gazette ltd, model NJ 2205;
saccharomyces cerevisiae (Saccharomyces cerevisiae) used in the examples was purchased from Kyork, Guangdong, Inc., model ATCC 9763;
propionibacterium acnes (Propionibacterium acnes) used in the examples were obtained from Kyork, Guangdong, Inc. of Microbiol. technology, model GIM 1.162.
Example 1A fermentation broth of a cosmetic composition (I) having anti-pollution effect and its preparation method
A traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 28 parts of sophora flower bud, 20 parts of ginkgo leaf, 17 parts of wild chrysanthemum flower, 18 parts of cocklebur fruit and 17 parts of liquorice.
The preparation method comprises the following steps:
adding composite bacteria in the form of bacteria liquid, wherein the composite bacteria liquid is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion of 3: 1 composition, the concentration of the compound bacterial liquid is 106～108cfu/mL。
Preparing materials: the total weight of the traditional Chinese medicines is 100g, and the traditional Chinese medicine raw materials are subjected to impurity removal, cleaning, drying, crushing to 100 meshes and uniformly mixing to obtain the traditional Chinese medicine composition; the preparation concentration is 106～108The pH value of the cfu/mL composite bacterial liquid is adjusted to 6.5-7.5.
③ fermentation: mixing the compound bacterial liquid, the traditional Chinese medicine composition and water according to the proportion, and fermenting for 40 hours in a shaking table at 32 ℃ and 100r/min to obtain fermentation liquid.
Purifying: filtering the obtained fermentation liquid, and autoclaving the filtrate at 110 deg.C for 30min to inactivate the strain. Centrifuging the sterilized fermentation filtrate for 30min under the conditions of 6000r/min and the centrifugal radius of 9cm, discarding the precipitate, collecting the supernatant, concentrating by using a membrane, adsorbing the concentrated solution by using macroporous resin (AB-8 macroporous adsorbent resin, purchased from Tianjin Pradde Biotechnology Co., Ltd.), eluting for multiple times by using 10-95% ethanol, collecting the eluent, concentrating by using the membrane to obtain the concentrated solution with the same weight as the traditional Chinese medicine composition, and adding butanediol with the same weight as the concentrated solution to dissolve, thus obtaining the traditional Chinese medicine composition fermentation liquor (I).
Wherein the dosage ratio of the composite bacterial liquid, the traditional Chinese medicine composition and water is 30 mL: 40 g: 300 g.
Example 2A cosmetic Chinese medicinal composition broth (II) with anti-pollution effect, and its preparation method
A traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 30 parts of sophora flower bud, 20 parts of ginkgo leaf, 20 parts of wild chrysanthemum flower, 20 parts of cocklebur fruit and 10 parts of liquorice.
The preparation method comprises the following steps:
adding composite bacteria in the form of bacteria liquid, wherein the composite bacteria liquid is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion of 3: 1 composition, the concentration of the compound bacterial liquid is 106～108cfu/mL。
Preparing materials: the total weight of the traditional Chinese medicines is 100g, and the traditional Chinese medicine raw materials are subjected to impurity removal, cleaning, drying, crushing to 80 meshes and uniformly mixing to obtain a traditional Chinese medicine composition; the preparation concentration is 106～108The pH value of the cfu/mL composite bacterial liquid is adjusted to 6.5-7.5.
③ fermentation: mixing the compound bacterial liquid, the traditional Chinese medicine composition and water according to the proportion, and fermenting for 48 hours in a shaking table at 35 ℃ and 100r/min to obtain fermentation liquid.
Purifying: filtering the obtained fermentation liquid, and autoclaving the filtrate at 110 deg.C for 30min to inactivate the strain. Centrifuging the sterilized fermentation filtrate for 30min under the conditions of 6000r/min and the centrifugal radius of 9cm, discarding the precipitate, collecting the supernatant, concentrating by using a membrane, adsorbing the concentrated solution by using macroporous resin, eluting for multiple times by using ethanol with the volume fraction of 10-95%, collecting the eluent, concentrating by using the membrane to obtain the concentrated solution with the same weight as the traditional Chinese medicine composition, adding butanediol with the same weight as the concentrated solution, dissolving, and filtering to obtain the traditional Chinese medicine composition fermentation liquor (II).
Wherein the dosage ratio of the composite bacterial liquid, the traditional Chinese medicine composition and water is 40 mL: 45 g: 400g
Example 3 fermentation broth (III) of cosmetic Chinese medicinal composition with anti-pollution effect, and preparation method thereof
A traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 25 parts of sophora flower bud, 15 parts of ginkgo leaf, 20 parts of wild chrysanthemum flower, 20 parts of cocklebur fruit and 20 parts of liquorice.
The preparation method comprises the following steps:
adding composite bacteria in the form of bacteria liquid, wherein the composite bacteria liquid is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion of 3: 1 composition, the concentration of the compound bacterial liquid is 106～108cfu/mL；
Preparing materials: the total weight of the traditional Chinese medicines is 100g, and the traditional Chinese medicine raw materials are subjected to impurity removal, cleaning, drying, crushing to 200 meshes and uniformly mixing to obtain a traditional Chinese medicine composition; the preparation concentration is 106～108The pH value of the cfu/mL composite bacterial liquid is adjusted to 6.5-7.5.
③ fermentation: mixing the compound bacterial liquid, the traditional Chinese medicine composition and water according to the proportion, and fermenting for 36 hours in a shaking table at 28 ℃ and 100r/min to obtain fermentation liquid.
Purifying: filtering the obtained fermentation liquid, and autoclaving the filtrate at 120 deg.C for 30min to inactivate the strain. Centrifuging the sterilized fermentation filtrate for 30min under the conditions of 8000r/min and the centrifugal radius of 9cm, discarding the precipitate, collecting the supernatant, concentrating by using a membrane, adsorbing the concentrated solution by using macroporous resin, eluting for multiple times by using ethanol with the volume fraction of 10-95%, collecting the eluent, concentrating by using the membrane to obtain the concentrated solution with the same weight as the traditional Chinese medicine, adding butanediol with the same weight as the concentrated solution, dissolving, and filtering to obtain the traditional Chinese medicine composition fermentation liquor (III).
Wherein the dosage ratio of the composite bacterial liquid, the traditional Chinese medicine composition and water is 25 mL: 30g of: 300 g.
Comparative examples 1-5 preparation of fermentation broth of single Chinese medicine
100g of Chinese herbal medicine materials of sophora flower bud, 100g of ginkgo leaf, 100g of wild chrysanthemum flower, 100g of cocklebur fruit and 100g of liquorice are respectively weighed. Fermenting and extracting according to the preparation method of the embodiment 1, and sequentially and respectively preparing single traditional Chinese medicine fermentation liquor.
Comparative example 6 Chinese medicinal composition extract
According to the dosage of the traditional Chinese medicine composition consistent with the embodiment 1, the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine components in parts by weight: 28 parts of sophora flower bud, 20 parts of ginkgo leaf, 17 parts of wild chrysanthemum flower, 18 parts of cocklebur fruit and 17 parts of liquorice.
The preparation method comprises the following steps: removing impurities from the traditional Chinese medicine raw materials, cleaning, drying, crushing to 100 meshes, and mixing the raw materials in a liquid-material mass ratio of 8: 1 adding deionized water, soaking for 2.5h, extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 60MPa, centrifugally separating an extracting solution, concentrating by using a membrane, adsorbing a concentrated solution by using macroporous resin, eluting for multiple times by using ethanol with the volume fraction of 10-95%, collecting an eluent, concentrating by using the membrane until the extracting solution is 100g, adding 100g of butanediol for dissolving, and filtering to obtain the extracting solution of the traditional Chinese medicine composition.
Test example 1 evaluation of efficacy in inhibiting Propionibacterium acnes
1. Preparation of Chinese medicine extract sample
The fermentation liquor of the traditional Chinese medicine composition for cosmetics with the anti-pollution effect, which is prepared in the embodiment 1, the single fermentation liquor of the traditional Chinese medicine prepared in the comparative examples 1-5 and the extracting solution of the traditional Chinese medicine composition prepared in the comparative example 6 are prepared into sample aqueous solutions with the mass fractions of 0.2%, 0.5%, 1.0%, 1.5% and 2.0% for later use.
2. Method for testing inhibition capacity of propionibacterium acnes
1mL of each of the 7 kinds of aqueous solutions was added to a sterile glass tube, and 8mL of a previously prepared modified GAM broth (Nissui, Japan, No. ZZM-05433) was poured. Taking out the purified CO and keeping the temperature of the CO constant at 35 DEG C2Culturing clinical Propionibacterium acnes in an incubator for 48h, respectively injecting 1mL of bacterial suspension into the modified GAM broth test tube containing the sample aqueous solution, and using a glass test tube containing no bacterial suspension as a negative control. Inoculating Propionibacterium acnes in each sample culture medium from low concentration, and placing at constant temperature of 35 deg.C CO as soon as possible after inoculation2The incubator is used for 48 hours. After the culture, the liquid in the improved GAM broth culture medium was first extracted for preliminary verification experiments, and then the kill of Propionibacterium acnes was determined by a VITEK full-automatic microbiological analysis system and an ANI anaerobe identification card, with the results as shown in Table 1 below.
TABLE 1 Propionibacterium acnes kill
The result shows that the cosmetic traditional Chinese medicine composition fermentation liquor with the anti-pollution effect has an obvious inhibition effect on propionibacterium acnes, the synergistic effect among the compositions is obvious, and the inhibition capability on propionibacterium acnes is obviously higher than that of the single traditional Chinese medicine fermentation liquor prepared in comparative examples 1-5. In addition, the bacteriostatic effect of the example 1 is obviously better than that of the comparative example 6, which shows that the bacteriostatic effect of the traditional Chinese medicine composition is obviously enhanced after fermentation.
Test example 2 evaluation of radical scavenging Effect
1. Preparation of the test specimens
The fermentation liquid of the Chinese medicinal composition prepared in the examples 1 to 3, the fermentation liquid of the single Chinese medicinal material prepared in the comparative examples 1 to 5, and the extraction liquid of the Chinese medicinal composition prepared in the comparative example 6 were mixed to prepare a sample aqueous solution with a mass fraction of 1%, and the following experiment was performed.
2. Evaluation of superoxide anion radical scavenging ability
4.5mL of Tris-HCl buffer (0.05 mol/L, pH 8.2) was preheated in a water bath at 25 ℃ for 20 min. Then adding 1mL of sample aqueous solution and 0.4mL of 25mmol/L pyrogallol solution, mixing uniformly, reacting in a water bath at 25 ℃ for 5min, and adding 1.0mL of 8mol/L HCl to terminate the reaction. Absorbance values were measured at 299nm with Tris-HCl buffer as a reference. Blank control the sample was replaced with 1mL of solvent of the aqueous sample solution. The clearance (D) was calculated according to equation (2).
Superoxide anion radical scavenging rate (%) [ 1- (a2/a1) ] × 100% (2)
Wherein A1 is the absorbance value of the blank control; a2 is the absorbance value of the drug.
3. Evaluation of hydroxyl radical scavenging ability
Sequentially adding 2mmol/L FeSO into a 25mL colorimetric tube4 3mL，1mmol/L H2O23mL, shaking up, then adding 3mL of salicylic acid with the concentration of 6mmol/L, shaking up, heating in a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance; adding the solutions to be tested at certain concentrations, shaking, heating in water bath for 15min, and collectingThe absorbance was measured. The following formula is the clearance rate of hydroxyl radical (OH) by the liquid to be tested:
hydroxyl radical clearance (%) ([ a 1-a 2- (a 1-A3) ]/a1 × 100% (3)
Wherein A1 is the absorbance value of the reaction system before adding medicine; a2 is the absorbance value of the system after the OH is removed by the medicine; a3 is the absorbance value of the system after blank control elimination of OH.
4. Results of the experiment
The anti-aging function evaluation test is shown in table 2.
TABLE 2 evaluation of anti-aging function test results
| Sample (I) | For superoxide anion radical clearance% | A hydroxyl radical clearance rate% |
| Example 1 | 95.6 | 92.7 |
| Example 2 | 93.8 | 91.2 |
| Example 3 | 94.6 | 90.7 |
| Comparative example 1 | 89.3 | 82.9 |
| Comparative example 2 | 88.6 | 84.2 |
| Comparative example 3 | 82.6 | 83.4 |
| Comparative example 4 | 62.4 | 42.6 |
| Comparative example 5 | 82.7 | 83.9 |
| Comparative example 6 | 84.2 | 82.3 |
The comparison of the experimental results of the examples 1 to 3 and the comparative examples 1 to 5 shows that the fermentation liquor of the traditional Chinese medicine composition can effectively remove free radicals and has a good anti-aging function; compared with the single traditional Chinese medicine fermentation liquor prepared in the proportion of 1-5, the free radical removing effect is good, and the effect of synergy is achieved after optimized combination. Comparison of experimental results of examples 1-3 and comparative example 6 shows that the effect of scavenging free radicals of the traditional Chinese medicine composition is remarkably enhanced after fermentation.
Test example 3 evaluation of anti-allergic Effect
The anti-allergic effect was evaluated by an inhibition test on hyaluronidase. Hyaluronidase (commercially available) is a hydrolase of hyaluronic acid and is closely linked to the development of inflammation and allergy. The inhibition effect of the plant anti-allergy moisturizing composition on skin allergy can be indirectly determined by measuring the inhibition rate of the plant anti-allergy moisturizing composition on hyaluronidase.
Test protocol: the inhibition of hyaluronidase by the extract was determined by the Elson-Morgan modification assay. 0.1mL of 0.25mmol/L CaCl is taken2The solution and 0.5mL of hyaluronidase solution are cultured for 20min at 37 ℃ in an insulation way; respectively adding 0, 0.5, 1.0, 1.5 and 2.0mL of the fermentation liquid or the extracting solution prepared in the example 1 and the comparative examples 1-6, and continuing to culture at 37 ℃ for 20 min; adding 0.5mL sodium hyaluronate solution, keeping the temperature at 37 ℃ for 30min, and standing at normal temperature for 5 min; adding 0.1mL of 0.4mol/L NaOH solution and 0.5mL of acetylacetone solution, heating in a boiling water bath for 15min, and immediately cooling for 5min with ice water; adding 1mL of Ellisib reagent, diluting to 5mL with anhydrous ethanol, standing for 20min for color development, and measuring the absorbance value with a spectrophotometer.
The hyaluronidase inhibition of the extract was calculated as follows:
the inhibition ratio (%) - (A-B) - (C-D) ]/(A-B) × 100%
Wherein, A is the absorbance of a control solution (sodium hyaluronate + hyaluronidase + deionized water); b is the absorbance of a control blank solution (acetic acid buffer solution + deionized water); c is the absorbance of the sample solution (potassium hyaluronate + hyaluronidase + sample solution); d is the absorbance of the sample blank solution (acetic acid buffer solution + sample solution).
The test results are shown in Table 3.
TABLE 3 results of hyaluronidase inhibition
The comparison of the experimental results of the examples 1-3 and the comparative examples 1-5 shows that the traditional Chinese medicine composition fermentation liquor can strongly inhibit hyaluronidase and has a good anti-allergy function; compared with the single traditional Chinese medicine fermentation liquor prepared in the proportion of 1-5, the hyaluronidase inhibition effect is good, and the optimized combination plays a role in synergy. The comparison of the experimental results of the examples 1-3 and the comparative example 6 shows that the hyaluronidase inhibition effect of the traditional Chinese medicine composition is remarkably enhanced after fermentation.
Test example 4 irritation evaluation test
And evaluating the irritation of the fermentation liquor by adopting a rabbit erythrocyte hemolysis experiment. The specific operation is as follows: and (3) respectively taking 500 mu L of the fermentation liquid or the extracting solution prepared in comparative examples 1-6 and examples 1-3, placing the 500 mu L of the fermentation liquid or the extracting solution in a 2mL centrifuge tube, respectively adding 500 mu L of 2% rabbit red blood cell (commercially available) suspension, shaking gently and uniformly mixing, placing the mixture in a constant temperature incubator at 37 ℃ for incubation for 3h, centrifuging at 2000r/min for 3min, absorbing supernatant, taking physiological saline as negative control and distilled water as positive control, measuring the absorbance at the wavelength of 414nm, and calculating the hemolysis according to a formula. The results are shown in Table 4.
TABLE 4 results of hemolysis
The comparison of the experimental results of the examples 1 to 3 and the comparative examples 1 to 5 shows that the hemolysis ratio of the fermentation liquor of the traditional Chinese medicine composition is much lower than that of the single traditional Chinese medicine prepared by the comparative examples 1 to 5, and the irritation is much lower. The comparison of the experimental results of the examples 1-3 and the comparative example 6 shows that the irritation of the fermentation liquor of the traditional Chinese medicine composition is also obviously reduced after fermentation.
Application example 1 anti-contamination mask
An antipollution mask containing the Chinese medicinal composition fermentation broth with the antipollution effect has the formula shown in table 5.
TABLE 5 anti-pollution facial mask formula
| Components | Mass fraction/% |
| The Chinese medicinal composition fermentation liquor (I) of the invention | 5 |
| Glycerol | 6.00 |
| 1, 3-propanediol | 4.00 |
| Hyaluronic acid sodium salt | 0.10 |
| Xanthan gum | 0.10 |
| Hydroxyethyl cellulose | 0.10 |
| Glycyrrhizic acid dipotassium salt | 0.50 |
| Aloe extract | 1.00 |
| 1, 2-hexanediol | 0.80 |
| P-hydroxyacetophenone | 0.50 |
| Deionized water | Balance of |
The preparation method comprises the following steps:
(1) dispersing xanthan gum and sodium hyaluronate in glycerol in advance, adding into deionized water together with dipotassium glycyrrhizinate, hydroxyethyl cellulose and Chinese medicinal composition fermentation broth (I), stirring and dissolving until uniformly dispersed and transparent;
(2) heating and dissolving p-hydroxyacetophenone in 1, 3-propylene glycol and 1, 2-hexanediol in advance, putting the mixture into the solution in the step (1), and stirring and dissolving the mixture uniformly;
(3) adding Aloe extract, stirring to dissolve completely;
(4) and (5) inspecting the physicochemical indexes such as color, pH, fragrance and appearance, filtering with 200 meshes after passing inspection, and discharging.
Application example 2 anti-pollution skin cream
Table 6 shows the formulation of an anti-fouling skin cream containing the fermented broth of the Chinese medicinal composition of the present invention.
TABLE 6 anti-pollution skin cream formula
| Composition (I) | Mass fraction (%) |
| Arachidyl alcohol glucoside | 1.5 |
| PEG-100 stearate | 1.2 |
| Glycerol stearate | 1 |
| Cetearyl glucoside | 0.8 |
| Shea butter | 0.5 |
| Potentilla chinensis seed oil | 3 |
| Hydrogenated polyisobutenes | 3 |
| Cyclopolydimethylsiloxane | 4 |
| Polydimethylsiloxane | 2 |
| Hydroxy phenyl methyl ester | 0.2 |
| Propyl hydroxybenzoate | 0.08 |
| EDTA‐2Na | 0.02 |
| Glycerol | 5 |
| 1, 3-butanediol | 3 |
| Hyaluronic acid sodium salt | 0.05 |
| Carbomer (CA) | 0.2 |
| Phenoxyethanol | 0.3 |
| Aminomethyl propanol | 0.12 |
| Essence | 0.1 |
| The Chinese medicinal composition fermentation liquor (I) of the invention | 5 |
| Deionized water | Balance of |
The preparation method comprises the following steps:
(1) 1, 3-butanediol and sodium hyaluronate are stirred uniformly in advance until the mixture is transparent, and carbomer is prepared into water dispersion with the mass fraction of 1%;
(2) adding oil soluble materials (arachidonoyl glucoside, PEG-100 stearate, glyceryl stearate, cetearyl glucoside, shea butter, white Potentilla seed oil, hydrogenated polyisobutene, cyclomethicone, dimethicone, methyl hydroxybenzoate, propyl hydroxybenzoate) into oil phase pot, heating to 80 deg.C, and stirring to dissolve completely to obtain oil phase;
(3) putting the 1, 3-butanediol processed in the step (1), sodium hyaluronate, the traditional Chinese medicine composition fermentation liquor (I) and other water phase raw materials (EDTA-2 Na, glycerol and aminomethyl propanol) into an emulsifying pot, heating to 80 ℃, and stirring until the raw materials are completely dissolved to form a water phase;
(4) rapidly pumping the oil phase into the emulsifying pot in the step (3), after the oil phase is added, homogenizing and emulsifying at the speed of 4000-6000 rpm for 5-10 minutes, and after the emulsification is finished, continuously stirring at the speed of 1000-2000 rpm and cooling;
(5) cooling to 65 ℃, adding the carbomer water dispersion with the mass fraction of 1% prepared in the step (1), and homogenizing at the speed of 4000-6000 rpm for 2 minutes;
(6) cooling to 45 deg.C, adding essence and phenoxyethanol, stirring to dissolve uniformly;
(7) and (3) after the temperature is reduced to 35 ℃, carrying out vacuum defoaming, checking physicochemical indexes such as color, fragrance and appearance, filtering with 200 meshes after the inspection is qualified, and discharging.
Application comparative example 1 general facial mask
Except that the fermentation broth (I) of the Chinese medicinal composition of the invention is not contained, the other formulation components, the dosage and the preparation method are the same as those of the application example 1.
Effect example 1 acne removing Effect, irritation evaluation and evaluation of skin elasticity of human body
Evaluation target: 60 young people with acne on the face and the age of 15-30 years are selected, wherein 30 male people and 30 female people are randomly and evenly divided into a group A and a group B, and each group contains 30 people.
The evaluation method comprises the following steps: by adopting a double-blind method, the group A uses the anti-pollution facial mask prepared in the application example 1 in the morning and evening of each day, and the group B uses the common facial mask prepared in the application comparative example 1 in the morning and evening of each day; and taking down the mask, and massaging the face until the mask liquid is completely absorbed by the skin.
Evaluation course of treatment: the acne removing effect evaluation takes 20 days as a treatment course, the irritation evaluation takes 20 days as a treatment course, and the human skin elasticity evaluation takes 7 weeks as a treatment course.
Evaluation standard of acne removing effect: the method has the following advantages: acne is faded by more than 70 percent; secondly, the method is effective: acne is faded by more than 30 percent; ③ invalid: the acne is faded by less than 30 percent.
Irritation evaluation criteria: the examination was performed according to the criteria of Table 7. The experiment was carried out for a total of 20 days.
TABLE 7 irritation evaluation criteria
| Grade | Identification criteria |
| 0 | Negative reaction: has no irritation and erythema |
| 1 | SuspiciousReaction: mild erythema |
| 2 | Weak positive reaction: erythema |
| 3 | Strong positive reaction: erythema, papules, blisters |
| 4 | Extremely strong positive reaction: severe edema and bulla |
Evaluation standard of human skin elasticity: skin elasticity tests were performed using a skin elasticity tester at weeks 2, 4, and 6 after application of the mask, and the average increase rate of skin elasticity was calculated for each group of volunteers.
Evaluation results of acne removal effects: the anti-pollution mask prepared by the application example 1 has the remarkable effect of 6 cases, accounting for 20 percent; 15 effective cases account for 50%; the number of the ineffective 9 cases is 30 percent. The total effective rate reaches 70 percent; the common facial mask prepared by applying the comparative example 1 has 0 effective case, 2 effective cases and 28 ineffective cases, and the effective rate is only 6.7%. The fermentation liquor of the traditional Chinese medicine composition has good acne removing effect.
Evaluation results of irritation: as shown in table 8.
TABLE 8 evaluation results of irritation
| Grade of allergy | Application comparative example 1 | Application example 1 |
| 0 grade number of people | 25 | 30 |
| 1 rank number of people | 4 | 0 |
| 2 grade number of people | 1 | 0 |
| 3 class number of people | 0 | 0 |
| 4 rank number of people | 0 | 0 |
As can be seen from the results in table 8, the common facial mask prepared in the application comparative example 1 without the addition of the fermentation broth of the traditional Chinese medicine composition of the present invention has an allergy phenomenon, and the anti-pollution facial mask prepared in the application example 1 with the addition of the fermentation broth of the traditional Chinese medicine composition of the present invention has an anti-allergy effect without an allergy phenomenon.
Human skin elasticity evaluation results: as shown in table 9.
Table 9 skin elasticity test results
As can be seen from table 9, the skin elasticity increase rate of the volunteers after using the anti-pollution mask prepared in application example 1 to which the Chinese medicinal composition fermentation broth of the present invention was added was significantly improved compared to the common mask prepared in application comparative example 1 to which the Chinese medicinal composition fermentation broth of the present invention was not added, and the anti-aging effect was excellent.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (13)
1. A traditional Chinese medicine composition fermentation liquor with an anti-pollution effect for cosmetics is characterized in that: the traditional Chinese medicine composition fermentation liquor for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 10-40 parts of sophora flower bud, 5-30 parts of ginkgo leaf, 5-30 parts of wild chrysanthemum flower, 5-30 parts of cocklebur fruit and 5-30 parts of liquorice;
the traditional Chinese medicine composition fermentation liquor with the anti-pollution effect for the cosmetics adopts a fermentation extraction method, namely, the traditional Chinese medicine composition is subjected to fermentation treatment, and then is subjected to filtration, sterilization and centrifugal separation to obtain the traditional Chinese medicine composition fermentation liquor;
the method specifically comprises the following steps:
firstly, adding composite bacteria in the form of bacteria liquid, wherein the composite bacteria liquid is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion (1-5): 1 composition, the concentration of the compound bacterial liquid is 106～108 cfu/mL；
Preparing materials: removing impurities from the traditional Chinese medicine raw materials, cleaning, drying, crushing to 80-200 meshes, and mixing uniformly to obtain a traditional Chinese medicine composition; the preparation concentration is 106～108 Adjusting the pH value of cfu/mL composite bacterial liquid to 6.5-7.5;
③ fermentation: the compound bacterial liquid, the traditional Chinese medicine composition and water are mixed according to the proportion of 20 mL-60 mL: 20 g-60 g: mixing 200-600 g of the mixture, and fermenting for 36-48 hours at the temperature of 28-42 ℃ and at the speed of 50-300 r/min to obtain fermentation liquor;
purifying: filtering the obtained fermentation liquor, and autoclaving the filtrate at 105-120 ℃ for 20-35 min to inactivate the strains; centrifuging the sterilized fermentation filtrate for 20-35 min under the conditions of 3000-8000 r/min and a centrifugation radius of 5-20 cm, discarding the precipitate, collecting the supernatant, concentrating by using a membrane, adsorbing the concentrated solution by using macroporous resin, eluting for multiple times by using ethanol with a volume fraction of 10-95%, collecting the eluent, concentrating by using the membrane to obtain a concentrated solution with the same weight as the traditional Chinese medicine composition, adding butanediol with the same weight as the concentrated solution, dissolving, and filtering to obtain the traditional Chinese medicine composition fermentation liquor for cosmetics with the anti-pollution effect.
2. The anti-pollution fermentation broth of a cosmetic traditional Chinese medicine composition according to claim 1, wherein:
the traditional Chinese medicine composition fermentation liquor with the anti-pollution effect for cosmetics is prepared from the following traditional Chinese medicine components in parts by weight: 25-30 parts of sophora flower bud, 15-20 parts of ginkgo leaf, 17-20 parts of wild chrysanthemum flower, 18-20 parts of cocklebur fruit and 10-20 parts of liquorice.
3. The preparation method of the fermentation liquor of the traditional Chinese medicine composition for cosmetics with anti-pollution effect as claimed in any one of claims 1 to 2, is characterized in that:
adopts a fermentation extraction method, namely, after the traditional Chinese medicine composition is fermented, the traditional Chinese medicine composition is filtered, sterilized and centrifugally separated to obtain traditional Chinese medicine composition fermentation liquor;
the method specifically comprises the following steps:
firstly, adding composite bacteria in the form of bacteria liquid, wherein the composite bacteria liquid is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion (1-5): 1 composition, the concentration of the compound bacterial liquid is 106～108 cfu/mL；
Preparing materials: removing impurities from the traditional Chinese medicine raw materials, cleaning, drying, crushing to 80-200 meshes, and mixing uniformly to obtain a traditional Chinese medicine composition; the preparation concentration is 106～108 Adjusting the pH value of cfu/mL composite bacterial liquid to 6.5-7.5;
③ fermentation: the compound bacterial liquid, the traditional Chinese medicine composition and water are mixed according to the proportion of 20 mL-60 mL: 20 g-60 g: mixing 200-600 g of the mixture, and fermenting for 36-48 hours at the temperature of 28-42 ℃ and at the speed of 50-300 r/min to obtain fermentation liquor;
purifying: filtering the obtained fermentation liquor, and autoclaving the filtrate at 105-120 ℃ for 20-35 min to inactivate the strains; centrifuging the sterilized fermentation filtrate for 20-35 min under the conditions of 3000-8000 r/min and a centrifugation radius of 5-20 cm, discarding the precipitate, collecting the supernatant, concentrating by using a membrane, adsorbing the concentrated solution by using macroporous resin, eluting for multiple times by using ethanol with a volume fraction of 10-95%, collecting the eluent, concentrating by using the membrane to obtain a concentrated solution with the same weight as the traditional Chinese medicine composition, adding butanediol with the same weight as the concentrated solution, dissolving, and filtering to obtain the traditional Chinese medicine composition fermentation liquor for cosmetics with the anti-pollution effect.
4. The method for preparing the fermentation liquor of the traditional Chinese medicine composition for cosmetics with anti-pollution effect according to claim 3, which is characterized in that:
the dosage ratio of the compound bacterial liquid, the traditional Chinese medicine composition and water is 25-40 mL: 30 g-45 g: 300-400 g;
the composite bacterial liquid in the step I is prepared from lactobacillus plantarum and saccharomyces cerevisiae according to the proportion of 3: 1, preparing a composition;
and step three, fermenting for 36-48 hours at the temperature of 28-35 ℃ and at the speed of 100r/min to obtain fermentation liquor.
5. The application of the fermentation liquor of the traditional Chinese medicine composition for cosmetics with the anti-pollution effect as defined in any one of claims 1-2 in the preparation of cosmetics.
6. Use according to claim 5, characterized in that:
the addition amount of the traditional Chinese medicine composition fermentation liquor for cosmetics with the anti-pollution effect in the cosmetics is 0.1-15% by mass.
7. A cosmetic characterized by: the fermentation liquor of the cosmetic traditional Chinese medicine composition with the anti-pollution effect contains the fermentation liquor of any one of claims 1-2.
8. The cosmetic according to claim 7, characterized in that:
the cosmetic contains 0.1-15% of a traditional Chinese medicine composition fermentation liquor for cosmetics with an anti-pollution effect in percentage by mass;
the cosmetic is as follows: emulsions, creams, gels, powders, masks, sprays, essences.
9. The cosmetic according to claim 7, characterized in that:
the cosmetic contains 0.1-15% of a traditional Chinese medicine composition fermentation liquor for cosmetics with an anti-pollution effect in percentage by mass;
the cosmetic is as follows: a water aqua.
10. The cosmetic according to claim 7, characterized in that:
the cosmetic contains 0.1-15% of a traditional Chinese medicine composition fermentation liquor for cosmetics with an anti-pollution effect in percentage by mass;
the cosmetic is as follows: powder molding.
11. The cosmetic according to claim 7, characterized in that:
the cosmetic contains 0.1-15% of a traditional Chinese medicine composition fermentation liquor for cosmetics with an anti-pollution effect in percentage by mass;
the cosmetic is as follows: washing and protecting.
12. The cosmetic according to claim 7 or 8, characterized in that:
the cosmetic is an anti-pollution mask and comprises the following components in parts by mass: 5% of Chinese medicinal composition fermentation liquor, 6.00% of glycerol, 4.00% of 1, 3-propylene glycol, 0.10% of sodium hyaluronate, 0.10% of xanthan gum, 0.10% of hydroxyethyl cellulose, 0.50% of dipotassium glycyrrhizinate, 1.00% of aloe extract, 0.80% of 1, 2-hexanediol, 0.50% of p-hydroxyacetophenone and the balance of deionized water.
13. The cosmetic according to claim 7 or 8, characterized in that:
the cosmetic is an anti-pollution skin cream and comprises the following components in parts by mass: arachidyl alcohol glucoside 1.5%, PEG-100 stearate 1.2%, glyceryl stearate 1%, cetearyl glucoside 0.8%, shea butter 0.5%, meadowfoam seed oil 3%, hydrogenated polyisobutene 3%, cyclomethicone 4%, dimethicone 2%, methylparaben 0.2%, propylparaben 0.08%, EDTA-2Na 0.02%, glycerin 5%, 1, 3-butylene glycol 3%, sodium hyaluronate 0.05%, carbomer 0.2%, phenoxyethanol 0.3%, aminomethyl propanol 0.12%, essence 0.1%, traditional Chinese medicine composition fermentation liquor 5%, and deionized water in balance.
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| CN111481492B (en) * | 2020-06-12 | 2022-07-26 | 广州鹰远生物科技有限公司 | Composition containing traditional Chinese medicine compound extract and application thereof |
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