CN111686179B - Traditional Chinese medicine compound extract with acne removing and red blood streak removing effects and application thereof - Google Patents

Traditional Chinese medicine compound extract with acne removing and red blood streak removing effects and application thereof Download PDF

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CN111686179B
CN111686179B CN202010543057.1A CN202010543057A CN111686179B CN 111686179 B CN111686179 B CN 111686179B CN 202010543057 A CN202010543057 A CN 202010543057A CN 111686179 B CN111686179 B CN 111686179B
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chinese medicine
red blood
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acne
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CN111686179A (en
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龚盛昭
朱永闯
石磊
徐梦漪
李港宁
方卓敏
王枫
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GUANGZHOU HOTO TRADE Co.,Ltd.
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Abstract

The invention discloses a traditional Chinese medicine compound extract with acne removing and red blood streak removing effects and application thereof, and belongs to the field of fine chemical engineering. The traditional Chinese medicine compound extract is prepared from the following traditional Chinese medicine components in parts by weight: 5-60 parts of ramie, 5-50 parts of root of canna edulis, 5-50 parts of sophora flower bud and 5-50 parts of xanthium sibiricum. The invention takes the monarch, minister, assistant and guide theory of traditional Chinese medicine as guidance to carry out prescription, and achieves the aims of simultaneously removing acne and red blood streak by the synergistic effect of a plurality of traditional Chinese medicines. The method adopts a high-pressure homogeneous extraction method to extract the traditional Chinese medicine, and then the traditional Chinese medicine compound extract is prepared by ultrafiltration and nanofiltration treatment, the whole process is carried out at normal temperature, and the active ingredients are well kept. The traditional Chinese medicine compound extract can effectively remove whelks, micro-acne and red blood streak, is safe and non-irritant to skin, is used in skin external products, and has good acne removing and red blood streak removing effects.

Description

Traditional Chinese medicine compound extract with acne removing and red blood streak removing effects and application thereof
Technical Field
The invention relates to a traditional Chinese medicine compound extract, in particular to a traditional Chinese medicine compound extract with the effects of removing acne and red blood streak and a preparation method and application thereof, belonging to the field of fine chemical engineering.
Background
Whelk, also called acne, pimple and pimple, is a common sebaceous gland chronic disease, and the cause of the whelk is that sebaceous gland secretion is vigorous during the puberty, so that pores are blocked to form a core of acne, namely micro acne (small nodule). Micro comedones are not visible, and a micro comedone requires a 7 to 9 month growth period from formation to skin surface. Pimples occlude pores to induce inflammation, and are the starting source of papules, pustules, nodules, and cysts. The traditional treatment only removes superficial acne, and micro acne grows out after a period of time, so that the situation of repeated attack of the acne is formed. Therefore, the scientific acne removing method should be started from the following two aspects: firstly, the formation of micro acne is eliminated by utilizing the active substances for removing blood stasis and resolving masses, and secondly, the already formed acne is eliminated by utilizing the active substances for resisting bacteria and oxidation. However, most of the existing acne removing methods pay attention to removal of the formed acne, and pay no attention to removal of the acne, so that the acne is eliminated in a bud state.
In addition, the skin which is easy to grow acne is often accompanied with the appearance of red blood streak, so scientific acne removing and skin care should be simultaneously carried out for removing acne and red blood streak.
The invention utilizes the thought of treatment based on syndrome differentiation of traditional Chinese medicine and the theory of traditional Chinese medicine as guidance to carry out scientific compound prescription, regulates the metabolism of organisms from aspects of qi, blood, yin, yang and the like, and has strong pertinence and lasting and stable effect.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention mainly aims to provide a traditional Chinese medicine compound extract with the effects of removing acnes and red blood streak; the traditional Chinese medicine compound extract has the effects of removing whelks, micro-acne and red blood streak, is high in safety when being used in skin external products, and has the advantages of good stability and high safety.
The invention also aims to provide a preparation method of the traditional Chinese medicine compound extract with the effects of removing acne and removing red blood streak.
The invention further aims to provide application of the traditional Chinese medicine compound extract with the effects of removing acne and removing red blood streak.
The purpose of the invention is realized by the following technical scheme:
a traditional Chinese medicine compound extract with acne removal and red blood streak removal effects is prepared from the following traditional Chinese medicine components in parts by weight: 5-60 parts of ramie, 5-50 parts of root of canna edulis, 5-50 parts of sophora flower bud and 5-50 parts of xanthium sibiricum.
Preferably, the traditional Chinese medicine compound extract with the effects of removing acne and removing red blood streak is prepared from the following traditional Chinese medicine components in parts by weight: 15-50 parts of ramie, 10-40 parts of root of canna edulis, 10-40 parts of sophora flower bud and 10-40 parts of xanthium sibiricum.
Preferably, the traditional Chinese medicine compound extract with the effects of removing acne and removing red blood streak is prepared from the following traditional Chinese medicine components in parts by weight: 30-35 parts of ramie, 20-25 parts of root of canna edulis, 20-25 parts of sophora flower bud and 20 parts of xanthium sibiricum.
Preferably, the traditional Chinese medicine compound extract (I) with the effects of removing acne and red blood streak is prepared from the following traditional Chinese medicine components in parts by weight: 35 parts of ramie, 25 parts of root of canna edulis, 20 parts of sophora flower bud and 20 parts of xanthium sibiricum.
Preferably, the traditional Chinese medicine compound extract (II) with the effects of removing acne and red blood streak is prepared from the following traditional Chinese medicine components in parts by weight: 30 parts of ramie, 25 parts of root of canna edulis, 25 parts of sophora flower bud and 20 parts of xanthium sibiricum.
Preferably, the traditional Chinese medicine compound extract (III) with the effects of removing acne and red blood streak is prepared from the following traditional Chinese medicine components in parts by weight: 35 parts of ramie, 20 parts of root of canna edulis, 25 parts of sophora flower bud and 20 parts of xanthium sibiricum.
The second purpose of the invention is to provide a preparation method of the traditional Chinese medicine compound extract with the effects of removing acne and removing red blood streak.
The invention adopts the following technical scheme, which specifically comprises the following steps:
crushing the traditional Chinese medicine raw materials, respectively sieving the crushed raw materials by using a sieve with 20-120 meshes, and mixing the crushed raw materials to obtain a traditional Chinese medicine compound mixture;
secondly, adding an extraction solvent according to the liquid-material mass ratio of 2-20: 1, soaking for 0.5-6 h, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 5-50 MPa to obtain an extracting solution; the extraction solvent is a mixed solution of PEG-40 hydrogenated castor oil, 1, 3-butanediol and water;
and thirdly, after centrifugal separation of the extracting solution, carrying out ultrafiltration treatment on the centrifugate, and carrying out nanofiltration treatment on the ultrafiltration permeating liquid to obtain nanofiltration trapped liquid, namely the traditional Chinese medicine compound extract.
Preferably, in the step (i), the mixture is sieved by a sieve with 60-100 meshes, and further sieved by a sieve with 80 meshes.
Preferably, the mass ratio of the liquid to the material in the second step is 5-15: 1; further, the mass ratio of liquid to material is 10: 1.
Preferably, the soaking time in the step (II) is 2 hours.
Preferably, the extraction solvent in the step (II) is a mixed solution of PEG-40 hydrogenated castor oil, 1, 3-butanediol and water, and the mass fractions of the mixed solution are 0.1-5%, 20-60% and 35-79.9%, respectively. Further, the mass fractions thereof were 0.5%, 35%, and 64.5%, respectively.
Preferably, the extraction pressure in the step (II) is 9.5 MPa.
Preferably, the radius of the centrifugation in the third step is 8-12 cm, and further 10 cm.
Preferably, the centrifugation conditions in the third step are 500-4000 rpm and normal temperature centrifugation for 15-60 min; further, the mixture was centrifuged at 2000rpm at room temperature for 30 min.
Preferably, the ultrafiltration in step (c) is to remove microorganisms and macromolecular organic substances (such as proteins, cellulose, resins, etc.), colloids, pigments, suspended solids, etc. from the extract, and the nanofiltration is to remove water and electrolytes to achieve the concentration and conductivity reduction.
Specifically, the molecular interception of the ultrafiltration membrane used in the step III is 1000-2000 Da, and further is 1000 Da;
specifically, the molecular interception amount of the nanofiltration membrane used in the step III is 80-120 Da, and further 100 Da;
the normal temperature in the invention is 15-40 ℃.
The third purpose of the invention is to apply the traditional Chinese medicine compound extract with the effects of removing acne and removing red blood streak to a skin external product, and provide a skin external product with the effects of removing acne and removing red blood streak.
In order to achieve the purpose of the invention, the invention adopts the following technical scheme:
the application of the traditional Chinese medicine compound extract with the acne removing and red blood streak removing effects in preparing a skin external product is characterized in that the adding amount of the traditional Chinese medicine compound extract with the acne removing and red blood streak removing effects in the skin external product is 0.1-50% by mass, and preferably 12-25% by mass.
The invention also provides a skin external product with the effects of removing acnes and removing red blood streaks, which contains the traditional Chinese medicine compound extract with the effects of removing acnes and removing red blood streaks.
Preferably, the skin external product contains 0.1-50% (more preferably 12-25%) of the traditional Chinese medicine compound extract with the effects of removing acne and red blood streak by mass fraction.
The skin external product comprises: aqueous preparations, emulsions, creams, gels, powders, powder-forming preparations, facial masks, sprays, and essences.
Specifically, the invention provides an acne-removing and red blood streak-removing essence which comprises the following components in parts by mass:
Figure GDA0003133400800000031
Figure GDA0003133400800000041
specifically, the invention provides an emulsion for removing acne and red blood streak, which comprises the following components in parts by mass:
Figure GDA0003133400800000042
specifically, the invention provides a mask for removing acnes and red blood streaks, which comprises the following components in parts by mass:
Figure GDA0003133400800000043
Figure GDA0003133400800000051
the mechanism of the invention is as follows:
the invention takes the monarch, minister, assistant and guide theory of traditional Chinese medicine as guidance to carry out prescription, takes the ramie as monarch drug, has the efficacies of clearing away heat and toxic material, resolving masses and relieving swelling, simultaneously has good antibacterial, hemostatic and blood cooling effects, and has obvious effects on removing small acne and already formed whelk; the radix cannae edulis is used as a ministerial drug, has the effects of clearing away heat and toxic materials, removing toxic swelling and cooling blood, and assists the ramie to better clear away acne and acne which are formed; the sophora flower bud is an adjuvant drug, has very good effects of promoting blood circulation and cooling blood, has very strong capacity of removing free radicals, and has obvious curative effect on repairing acne-growing skin; the xanthium sibiricum is a guiding drug, has the effects of expelling wind, removing dampness and cold, warming and activating meridian, killing insects and sterilizing and the like, and has the effect of harmonizing the medicines. The medicines are used together to strengthen the body resistance, strengthen the vitality, nourish and condition, and take the effects of clearing heat and removing toxicity, eliminating stagnation and reducing swelling, and promoting blood circulation and cooling blood as the basis of removing acne and red blood streak; the insecticidal, bactericidal and free radical scavenging effects are taken as the targets of removing acne and red blood streak, and the synergy is realized.
Compared with the prior art, the invention has the following advantages and effects:
in order to obtain the best extraction effect, the invention firstly carries out scientific experiments on the extraction solvent, and respectively uses water, ethanol water solution and 1, 3-butanediol water solution as the extraction solvent, and as a result, the 1, 3-butanediol water solution has the best effect; in order to further improve the extraction efficiency, the PEG-40 hydrogenated castor oil and the 1, 3-butanediol aqueous solution are innovatively mixed to be used as an extraction solvent, and the extraction effect is obviously better than that of the 1, 3-butanediol aqueous solution. The extraction and separation processes are carried out at normal temperature, so as to ensure that the active ingredients do not lose activity due to heat treatment.
The acne-removing and red blood streak-removing powder is prepared by taking the monarch, minister, assistant and guide theory of the traditional Chinese medicine as guidance, and achieves the aims of removing acne and red blood streak from the aspects of removing stasis and detumescence, clearing heat and detoxicating, cooling blood and activating blood, preventing wind and dispelling wind, removing free radicals and the like through the synergistic effect of various traditional Chinese medicine active substances.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
The test methods in the following examples, in which specific experimental conditions are not specified, are generally performed according to conventional experimental conditions or according to the experimental conditions recommended by the manufacturer.
Propionibacterium acnes (Propionibacterium acnes) used in the examples were obtained from Kyork, Guangdong, Inc. of Microbiol. technology, model GIM 1.162.
Example 1A Compound extract of Chinese herbs with anti-acne and anti-redness effects (I)
A traditional Chinese medicine compound extract with acne removal and red blood streak removal effects is prepared from the following traditional Chinese medicine components in parts by weight: 35 parts of ramie, 25 parts of root of canna edulis, 20 parts of sophora flower bud and 20 parts of xanthium sibiricum. The total weight of the traditional Chinese medicine is 5 kg.
The preparation method comprises the following steps:
crushing the traditional Chinese medicine raw materials, respectively sieving the crushed raw materials by using a 80-mesh sieve, and mixing the crushed raw materials to obtain a traditional Chinese medicine compound mixture;
adding an extraction solvent according to the liquid-material mass ratio of 10:1, soaking for 2 hours, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 9.5MPa to obtain an extracting solution; the extraction solvent is a mixed solution of PEG-40 hydrogenated castor oil, 1, 3-butanediol and water, and the mass fractions are respectively 0.5%, 35% and 64.5%.
③ centrifuging the extracting solution for 30min at the normal temperature and 2000rpm under the condition that the centrifugal radius is 10cm, then carrying out ultrafiltration treatment on the centrifugate (the molecular interception is 1000Da), and carrying out nanofiltration treatment on the ultrafiltration permeating liquid (the molecular interception is 100Da) to obtain nanofiltration interception liquid, thus obtaining 20kg of the traditional Chinese medicine compound extract (I).
Example 2A Compound extract of Chinese herbs with anti-acne and anti-redness effects (II)
A traditional Chinese medicine compound extract with acne removal and red blood streak removal effects is prepared from the following traditional Chinese medicine components in parts by weight: 30 parts of ramie, 25 parts of root of canna edulis, 25 parts of sophora flower bud and 20 parts of xanthium sibiricum.
The preparation method is consistent with the example 1, and the traditional Chinese medicine compound extract (II) provided by the invention is prepared.
Example 3A Compound extract of Chinese herbs (III) having anti-acne and anti-redness effects
A traditional Chinese medicine compound extract with acne removal and red blood streak removal effects is prepared from the following traditional Chinese medicine components in parts by weight: 35 parts of ramie, 20 parts of root of canna edulis, 25 parts of sophora flower bud and 20 parts of xanthium sibiricum.
The preparation method is consistent with the embodiment 1, and the traditional Chinese medicine compound extract (III) provided by the invention is prepared.
Comparative examples 1-4 extracts of a single herb
100 parts of Chinese medicine ramie, 100 parts of root of canna edulis, 100 parts of sophora flower bud and 100 parts of xanthium sibiricum are respectively weighed. The extraction and separation were carried out according to the preparation method described in example 1, and the single herb extracts were prepared in sequence.
Comparative example 5 composite extract of Sophora japonica and Xanthium sibiricum
Weighing 50 parts of sophora flower bud and 50 parts of cocklebur. The extraction and separation are carried out according to the preparation method described in the example 1, and the composite extract of the sophora flower bud and the xanthium sibiricum is sequentially and respectively prepared.
Comparative example 61, 3-butanediol aqueous solution as extraction solvent of traditional Chinese medicine compound extract
The extraction solvent in example 1 was changed to a mixed solution of 1, 3-butanediol and water, and the mass fractions were 35% and 65%, respectively. The others correspond to example 1.
Test example 1 evaluation of efficacy in inhibiting Propionibacterium acnes
1. Preparation of Chinese medicine extract sample
The traditional Chinese medicine compound extract with the acne removing and red blood streak removing effects prepared in the examples 1-3 and the extracts prepared in the comparative examples 1-6 are prepared into sample aqueous solutions with the mass fractions of 0.5%, 1%, 2%, 3%, 4% and 5% for later use.
2. Method for testing inhibition capacity of propionibacterium acnes
1mL of each of the 9 sample aqueous solutions was added to a sterile glass tube, and 8mL of a previously prepared modified GAM broth (Nissui, Japan, No. ZZM-05433) was poured. Taking out the purified CO and keeping the temperature of the CO constant at 35 DEG C2Culturing clinical Propionibacterium acnes in an incubator for 48h, respectively injecting 1mL of bacterial suspension into the modified GAM broth test tube containing the sample aqueous solution, and using a glass test tube containing no bacterial suspension as a negative control. Inoculating Propionibacterium acnes in each sample culture medium from low concentration, and placing at constant temperature of 35 deg.C CO as soon as possible after inoculation2The incubator is used for 48 hours. After the culture, the liquid in the improved GAM broth culture medium was first extracted for preliminary verification experiments, and then the kill of Propionibacterium acnes was determined by a VITEK full-automatic microbiological analysis system and an ANI anaerobe identification card, with the results as shown in Table 1 below.
TABLE 1 Propionibacterium acnes kill
Figure GDA0003133400800000071
The comparison results of the examples 1-3 and the comparative examples 1-5 show that the traditional Chinese medicine compound extract prepared by the invention has an obvious inhibition effect on propionibacterium acnes, and has a much better effect than the single traditional Chinese medicine extract prepared by the comparative examples 1-4 and the composite extract of sophora flower bud and xanthium sibiricum prepared by the comparative example 5, which shows that the traditional Chinese medicine components have a remarkable synergistic effect after the compound.
The result of comparison between the example 1 and the comparative example 6 shows that the inhibition effect of the traditional Chinese medicine compound extract prepared by the extraction solvent adopted by the invention on propionibacterium acnes is better than that of the traditional Chinese medicine compound extract prepared by the solvent adopted by the comparative example 6, which indicates that the effect of the extraction solvent adopted by the invention is better.
Test example 2 radical scavenging test
1. Preparation of the test specimens
The traditional Chinese medicine compound extract with the acne removing and red blood streak removing effects prepared in the examples 1-3 and the extracts prepared in the comparative examples 1-6 are prepared into sample aqueous solutions with the mass fraction of 0.5%, and the following experiments are carried out.
2. Evaluation of hydroxyl radical scavenging ability
Sequentially adding 2mmol/L FeSO into a 25mL colorimetric tube4 3mL，1mmol/L H2O23mL, shaking up, then adding 3mL of salicylic acid with the concentration of 6mmol/L, shaking up, heating in a water bath at 37 ℃ for 15min, taking out, and measuring the absorbance; respectively adding 0.5mL of the solution to be tested, shaking, heating in water bath for 15min, and taking out to test its absorbance. The clearance of hydroxyl radical is calculated according to formula (1):
hydroxyl radical clearance (%) ([ a 1-a 2- (a 1-A3) ]/a1 × 100% (1)
Wherein A1 is the absorbance value of the reaction system before adding the sample; a2 is the absorbance value of the system after removing OH from the sample; a3 is the absorbance value of the system after blank control elimination of OH.
3. Results of the experiment
The results of the free radical scavenging experiments are shown in table 2.
TABLE 2 free radical scavenging test results
Sample (I) A hydroxyl radical clearance rate%
Example 1 83.1
Example 2 84.3
Example 3 84.8
Comparative example 1 61.9
Comparative example 2 52.6
Comparative example 3 71.5
Comparative example 4 51.8
Comparative example 5 72.9
Comparative example 6 76.1
The comparison results of examples 1-3 and comparative examples 1-5 show that the traditional Chinese medicine compound extract prepared by the invention has a much better effect on removing hydroxyl radicals than the single traditional Chinese medicine extract prepared by the comparative examples 1-4 and the sophora flower bud and xanthium sibiricum compound extract prepared by the comparative example 5, and the synergistic effect among the traditional Chinese medicine components after the compound is carried out is obvious.
The result of comparison between the example 1 and the comparative example 6 shows that the hydroxyl radical scavenging effect of the traditional Chinese medicine compound extract prepared by the extraction solvent adopted by the invention is better than that of the traditional Chinese medicine compound extract prepared by the solvent adopted by the comparative example 6, which indicates that the effect of the extraction solvent adopted by the invention is better.
Test example 3 irritation evaluation test
The irritation of the Chinese medicinal extract is evaluated by a rabbit erythrocyte hemolysis experiment. The specific operation is as follows: respectively taking 500 mu L of the traditional Chinese medicine extracts prepared in the examples 1-3 and the comparative examples 1-5, placing the extracts in a 2mL centrifuge tube, respectively adding 500 mu L of 2% rabbit red blood cell (commercially available) suspension, shaking gently and mixing uniformly, placing the mixture in a constant temperature incubator at 37 ℃ for incubation for 3h, centrifuging the mixture at 2000r/min for 3min, absorbing supernatant, taking physiological saline as negative control and distilled water as positive control, measuring absorbance at the wavelength of 414nm, and calculating the hemolysis according to a formula. The results are shown in Table 3.
TABLE 3 results for haemolysis
Figure GDA0003133400800000091
The comparison of the experimental results of the examples 1-3 and the comparative examples 1-5 shows that the hemolysis ratio of the traditional Chinese medicine compound extract is much lower than that of the extract prepared by the comparative examples 1-5, and the irritation of the traditional Chinese medicine extract after the compound is obviously reduced.
Application example 1 essence for removing acne and red blood streak
The acne-removing and redness-removing essence containing a traditional Chinese medicine compound extract with the effects of removing acne and redness-removing is shown in a formula shown in table 4.
TABLE 4
Composition (I) Mass fraction/%
Deionized water To 100
Glycerol 5.00
1, 3-propanediol 4.00
Hyaluronic acid sodium salt 0.10
Xanthan gum 0.07
Hydroxyethyl cellulose 0.05
Glycyrrhizic acid dipotassium salt 0.50
The traditional Chinese medicine compound extract (I) of the invention 12.00
1, 2-hexanediol 0.80
P-hydroxyacetophenone 0.50
The preparation method comprises the following steps:
(1) dispersing xanthan gum and sodium hyaluronate in glycerol in advance, then adding the dispersed xanthan gum and sodium hyaluronate, dipotassium glycyrrhizinate and hydroxyethyl cellulose into the traditional Chinese medicine compound extract (I) together, and stirring and dissolving until the dispersion is uniform and transparent;
(2) heating and dissolving p-hydroxyacetophenone in 1, 3-propylene glycol and 1, 2-hexanediol in advance, putting the mixture into the solution in the step (1), and stirring and dissolving the mixture uniformly;
(3) and (5) inspecting the physicochemical indexes such as color, pH, fragrance and appearance, filtering with 200 meshes after passing inspection, and discharging.
Application example 2 emulsion for removing acne and red blood streak
The acne-removing and redness-removing lotion contains a traditional Chinese medicine compound extract with the effects of removing acne and redness-removing, and the formula is shown in table 5.
TABLE 5
Figure GDA0003133400800000101
Figure GDA0003133400800000111
The preparation method comprises the following steps:
(1) heating the raw material of the component A to 80 ℃, and stirring and dispersing uniformly for later use;
(2) heating the raw material of the component B to 80 ℃, and stirring and dissolving uniformly;
(3) pumping the raw materials of the component B into the phase A for homogenizing and emulsifying, and cooling after the emulsification is finished;
(4) cooling to 45 ℃, adding the raw material of the component C, and stirring and dispersing uniformly. Vacuumizing and defoaming are finished.
Application example 3 acne-removing and redness-removing mask
The acne-removing and redness-removing facial mask containing a traditional Chinese medicine compound extract with the effects of removing acne and removing redness has a formula shown in table 6.
TABLE 6
Composition (I) Mass fraction/%
Deionized water To 100
Glycerol 7.00
1, 3-propanediol 7.00
Hyaluronic acid sodium salt 0.05
Glycyrrhizic acid dipotassium salt 0.50
Xanthan gum 0.10
Hydroxyethyl cellulose 0.10
Allantoin 0.25
Panthenol 0.50
1, 2-hexanediol 0.80
P-hydroxyacetophenone 0.50
The traditional Chinese medicine compound extract (I) of the invention 25.00
The preparation method comprises the following steps:
(1) dispersing xanthan gum and sodium hyaluronate in glycerol in advance, adding the dispersed xanthan gum and sodium hyaluronate, allantoin, panthenol, hydroxyethyl cellulose and dipotassium glycyrrhizinate into deionized water, heating to 80 ℃, stirring and dissolving until the mixture is uniform and transparent, and cooling;
(2) heating and dissolving p-hydroxyacetophenone in 1, 3-propylene glycol and 1, 2-hexanediol in advance until the p-hydroxyacetophenone is transparent, putting the p-hydroxyacetophenone into the solution obtained in the step (1) when the temperature of the system is reduced to 55 ℃, and stirring and dissolving the p-hydroxyacetophenone until the p-hydroxyacetophenone is transparent;
(3) when the temperature is reduced to 40 ℃, the traditional Chinese medicine compound extract (I) is put into the mixture and stirred uniformly until the mixture is completely dissolved;
(4) and (5) inspecting the physicochemical indexes such as color, pH, fragrance and appearance, filtering with 200 meshes after passing inspection, and discharging.
Effect example 1 evaluation of effects and evaluation of skin irritation
Evaluation target: 40 young people with whelk on the face (accompanied by a large amount of tiny acne) and the age of 15-30 years are selected, wherein 20 male people and 20 female people are selected.
The evaluation method comprises the following steps: the acne-removing and red blood streak-removing mask prepared in application example 3 is applied to the face in the morning and evening by a double-blind method, and the face is massaged after the mask is taken down until all mask liquid is absorbed by the skin.
Evaluation course of treatment: the acne removing effect evaluation takes 20 days as a treatment course, the micro powder and red blood streak removing effect evaluation takes 90 days as a treatment course, and the irritation evaluation takes 20 days as a treatment course.
Evaluation standard of acne removing effect: the method has the following advantages: acne eliminating rate over 70%; secondly, the method is effective: acne is eliminated by more than 30%; ③ invalid: acne is eliminated by less than 30%.
Evaluation standard of the effect of removing the micro-powder thorn: the method has the following advantages: micro acne is reduced by more than 70%; secondly, the method is effective: micro acne is reduced by more than 30%; ③ invalid: the micro acne is reduced by less than 30%.
Evaluation standard of effect of removing red blood streak: the red pigment values of the front and rear cheeks before and after use were tested with Mexamter MX18(CK Electronic GmH, Germany). The red blood streak removing effect is judged by testing the inhibition conditions (inhibition rate) of the red pigment before and after use, and the formula of the inhibition rate is as follows: inhibition (pre-use red pigment value-post-use red pigment value) × 100%/pre-use red pigment value; the method has the following advantages: the inhibition rate is more than or equal to 20 percent; the method has the following advantages: ② the inhibition rate is 5-20 percent; and (4) invalidation: the inhibition rate is less than 5%.
Irritation evaluation criteria: the examination was performed according to the criteria of Table 7.
TABLE 7 irritation evaluation criteria
Grade Identification criteria
0 Negative reaction: has no irritation and erythema
1 And (3) suspicious reaction: mild erythema
2 Weak positive reaction: erythema
3 Strong positive reaction: erythema, papules, blisters
4 Extremely strong positive reaction: severe edema and bulla
Evaluation results of acne removal effects: 18 cases of obvious effect account for 45 percent; 14 cases are effective, accounting for 35 percent; and 8 ineffective cases account for 20 percent. The total effective rate reaches 80 percent, which shows that the traditional Chinese medicine compound extract has good acne removing effect.
Evaluation results of the effect of removing the micro-powder thorn: 12 cases of obvious effect account for 30 percent; 23 cases of effectiveness account for 57.5 percent; and 5 invalid cases account for 12.5 percent. The total effective rate reaches 87.5 percent, which shows that the traditional Chinese medicine compound extract has good micro-powder thorn removing effect.
Evaluation results of the effect of removing red blood streak: 15 cases of obvious effect account for 37.5 percent; the effective 18 cases account for 45%; and 7 ineffective cases account for 17.5 percent. The total effective rate reaches 82.5 percent, which shows that the traditional Chinese medicine compound extract has good effect of removing red blood streak.
Evaluation results of irritation: no allergic phenomenon was found in all volunteers.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (11)

1. A traditional Chinese medicine compound extract with the effects of removing acne and red blood streak is characterized in that: the traditional Chinese medicine compound extract is prepared from the following traditional Chinese medicine components in parts by weight: 30-35 parts of ramie, 20-25 parts of root of canna edulis, 20-25 parts of sophora flower bud and 20 parts of xanthium sibiricum;
the preparation method of the traditional Chinese medicine compound extract with the effects of removing acne and red blood streak specifically comprises the following steps:
crushing the traditional Chinese medicine raw materials, respectively sieving the crushed raw materials by using a sieve with 20-120 meshes, and mixing the crushed raw materials to obtain a traditional Chinese medicine compound mixture;
secondly, adding an extraction solvent according to the liquid-material mass ratio of 2-20: 1, soaking for 0.5-6 h, and then extracting at normal temperature by using a high-pressure homogenizing extractor under the extraction pressure of 5-50 MPa to obtain an extracting solution; the extraction solvent is a mixed solution of PEG-40 hydrogenated castor oil, 1, 3-butanediol and water, and the mass fractions of the mixed solution are 0.5 percent, 35 percent and 64.5 percent respectively;
and thirdly, after centrifugal separation of the extracting solution, carrying out ultrafiltration treatment on the centrifugate, and carrying out nanofiltration treatment on the ultrafiltration permeating liquid to obtain nanofiltration trapped liquid, namely the traditional Chinese medicine compound extract.
2. The traditional Chinese medicine compound extract with the effects of removing acne and red blood streak according to claim 1, which is characterized in that:
screening by using a 60-100-mesh sieve;
the mass ratio of the liquid to the material in the second step is 5-15: 1;
the soaking time in the step II is 2 hours;
the extraction pressure in the step two is 9.5 MPa;
the radius of the centrifugation in the third step is 8-12 cm;
centrifuging at the normal temperature for 15-60 min at 500-4000 rpm;
the molecular interception of the ultrafiltration membrane used in the step (III) is 1000-2000 Da;
the molecular interception amount of the nanofiltration membrane used in the step (III) is 80-120 Da;
the normal temperature is 15-40 ℃.
3. The application of the traditional Chinese medicine compound extract with the effects of removing acne and red blood streak according to any one of claims 1-2 in preparing skin external products.
4. Use according to claim 3, characterized in that:
the addition amount of the traditional Chinese medicine compound extract with the acne removing and red blood streak removing effects in the skin external product is 0.1-50% by mass.
5. Use according to claim 4, characterized in that:
the addition amount of the traditional Chinese medicine compound extract with the acne removing and red blood streak removing effects in the skin external product is 12-25% by mass.
6. A skin external product with the effects of removing acne and red blood streak is characterized in that: the traditional Chinese medicine compound extract containing the traditional Chinese medicine compound extract with the effects of removing acne and red blood streak according to any one of claims 1-2.
7. The skin external product with the effects of removing acne and red blood streak according to claim 6, wherein:
the skin external product contains 0.1-50% of a traditional Chinese medicine compound extract with the effects of removing acne and red blood streak by mass fraction;
the skin external product comprises: emulsions, creams, gels, powders, masks, sprays, and essences.
8. The skin external product with the effects of removing acne and red blood streak according to claim 6, wherein:
the skin external product contains 0.1-50% of a traditional Chinese medicine compound extract with the effects of removing acne and red blood streak by mass fraction;
the skin external product comprises: aqueous solution and powder molding.
9. The skin external product with the effects of removing acne and red blood streak according to claim 6, wherein:
the skin external product is an essence for removing acnes and red blood streaks, and comprises the following components in parts by mass: 5.00 percent of glycerin, 4.00 percent of 1, 3-propylene glycol, 0.10 percent of sodium hyaluronate, 0.07 percent of xanthan gum, 0.05 percent of hydroxyethyl cellulose, 0.50 percent of dipotassium glycyrrhizinate, 12.00 percent of Chinese herbal compound extract, 0.80 percent of 1, 2-hexanediol, 0.50 percent of p-hydroxyacetophenone and 100 percent of deionized water.
10. The skin external product with the effects of removing acne and red blood streak according to claim 6, wherein:
the skin external product is an emulsion for removing acnes and red blood streaks, and comprises the following components in parts by mass: 0.02% of EDTA-2Na, 3.00% of dipotassium glycyrrhizinate, 0.50% of 1, 3-butanediol, 2.00% of 1, 2-propanediol, 0.80% of 1, 2-hexanediol, 0.50% of p-hydroxyacetophenone, 0.20% of carbomer, 0.05% of sodium hyaluronate, 2.50% of polyglycerol-3 methyl glucose distearate, 0.50% of sorbitan sesquioleate, 2.50% of isopropyl palmitate, 2.50% of meadowfoam seed oil, 1.50% of shea butter, 0.50% of tocopherol acetate, 3.50% of cyclopenta dimethyl siloxane, 15.00% of a traditional Chinese medicine compound extract, 0.10% of aminomethyl propanol, 0.10% of essence and 100% of deionized water.
11. The skin external product with the effects of removing acne and red blood streak according to claim 6, wherein:
the skin external product is a mask for removing acnes and red blood streaks, and comprises the following components in parts by mass: 7.00 percent of glycerin, 7.00 percent of 1, 3-propylene glycol, 0.05 percent of sodium hyaluronate, 0.10 percent of xanthan gum, 0.10 percent of hydroxyethyl cellulose, 0.25 percent of allantoin, 0.50 percent of panthenol, 0.50 percent of dipotassium glycyrrhizinate, 0.80 percent of 1, 2-hexanediol, 0.50 percent of p-hydroxyacetophenone, 25.00 percent of compound traditional Chinese medicine extract and 100 percent of deionized water.
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CN103751074A (en) * 2014-02-26 2014-04-30 广东轻工职业技术学院 Compound traditional Chinese medicine extract with effect of removing red blood silk, and application
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Publication number Priority date Publication date Assignee Title
CN103751074A (en) * 2014-02-26 2014-04-30 广东轻工职业技术学院 Compound traditional Chinese medicine extract with effect of removing red blood silk, and application
CN105833187A (en) * 2016-05-14 2016-08-10 广东轻工职业技术学院 Botanical medicine composition with acne removing effect and application

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