CN111449997A - A cosmetic containing chlorella extract as main active ingredient - Google Patents
A cosmetic containing chlorella extract as main active ingredient Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
- A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
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Abstract
The invention provides a cosmetic containing green algae extract as active ingredient, which is prepared by dissolving green algae powder in hot water, and extracting at high temperature; centrifuging the extracted sample and taking supernatant; filtering the supernatant with a filter membrane, and freeze-drying the filtrate to obtain dry extract powder; dissolving the dry powder, and precipitating with absolute ethyl alcohol; freeze drying the precipitate to obtain Chlorella extract. The green alga extract provided by the invention is used for preparing moisturizing and antibacterial products with chitosan oligosaccharide. The invention provides a cosmetic with moisturizing and bacteriostatic effects, wherein the active ingredients of the cosmetic comprise green algae extract, and the green algae extract is purified and added into the cosmetic, so that the cosmetic can activate cell functions, enhance skin cell metabolism, promote skin moisture circulation, increase skin elasticity and improve skin quality.
Description
Technical Field
The invention belongs to the technical field of cosmetics, and particularly relates to a cosmetic using a green alga extract as a main active ingredient.
Background
In the field of cosmetics, skin moisturizing is a basic care work, and after skin moisturizing is done, skin aging can be delayed, and skin diseases can be reduced and prevented, so that moisturizing cosmetics with safe efficacy have great market demands. The human body mainly relies on a natural moisturizing system consisting of water, lipids and natural moisturizing factors to maintain the healthy state of the skin, and the moisturizing agent in the cosmetics plays a role in simulating the system, providing temporary protection for the skin, reducing damage and promoting repair.
At present, the traditional moisturizers mainly comprise glycerin, propylene glycol, butylene glycol, sorbitol, sodium pyrrolidone carboxylate, polyethylene glycol, sodium lactate and the like, are synthesized by chemical methods, and are widely used cosmetic moisturizers at present. However, the chemically synthesized moisturizers have certain application limitations, for example, glycerin has strong hygroscopicity, and absorbs water from skin when the environment is dry, so the proportioning of glycerin determines the moisturizing effect of the moisturizers; the final product of sodium lactate is not easy to emulsify; the polyol is used in a small amount because of its irritant reaction caused by its high concentration, and has a limited moisturizing effect. The conventional humectant can not meet the pursuit of people on high-quality, natural and safe cosmetics.
The green algae extract is the unique active component of green algae, and the green algae is increased by 4 times every 20h and is mainly determined by the active component in the green algae extract. The main components of the green algae extract include amino acids, aminopeptide, water soluble protein, conjugated protein, glycoprotein, vitamins, minerals, glutathione-like substances, RNA/DNA, immune polysaccharide, etc. The green algae extract has the effects of promoting normal cell proliferation, enhancing organism immunity, promoting human body RNA/DNA to renew, repair, prevent aging, enhancing cell effect, protecting cells, removing toxin, resisting oxidation, resisting inflammation, resisting radiation, resisting infection, etc. The green algae extract has water solubility of 100%, is easy to be absorbed, and can be applied in cosmetics for enhancing skin cell metabolism, promoting skin water circulation, and caring water.
However, the existing extraction method mainly comprises an enzymolysis method, a high-pressure hot water extraction method and an ultrasonic water extraction method, and the extracting solution contains impurities such as pigment, so that the skin safety hidden trouble exists when the extracting solution is added into a skin care product, and the effect of active ingredients cannot be realized.
Disclosure of Invention
The invention aims to provide a cosmetic with an active ingredient containing a green alga extract, namely an extracting solution obtained by separating and purifying an effective ingredient from green alga has good biocompatibility, can increase skin moisture, reduce wrinkles and improve the skin quality of a user.
The invention firstly provides a green alga extract, and the preparation method comprises the following steps:
dissolving green algae powder in hot water, and extracting at high temperature; centrifuging the extracted sample and taking supernatant; filtering the supernatant with a filter membrane, and freeze-drying the filtrate to obtain dry extract powder; dissolving the dry powder, and precipitating with absolute ethyl alcohol; freeze drying the precipitate to obtain green algae extract;
the green alga extract provided by the invention is applied to preparing moisturizing and antibacterial products; the product is preferably a cosmetic.
In another aspect, the invention provides a moisturizing and antibacterial product, which uses green algae extract and chitosan oligosaccharide in the raw materials for preparation;
the mass ratio of the chlorella extract to the chitosan oligosaccharide in the cosmetic is 1-15: 1; preferably 1-5: 1;
the cosmetic is also added with other components;
the components comprise any one or more of propylene glycol, glycerol, squalane, stearic acid, cetanol, stearyl alcohol, polysorbate, sorbitan stearate, isopropyl palmitate and triethanolamine.
Compared with the existing conventional component cosmetics, the invention has the following advantages:
1. the invention provides a cosmetic with moisturizing and bacteriostatic effects, wherein the active ingredients of the cosmetic comprise green algae extract, and the green algae extract is purified and added into the cosmetic, so that the cosmetic can activate cell functions, enhance skin cell metabolism, promote skin moisture circulation, increase skin elasticity and improve skin quality.
2. The invention provides a cosmetic with moisturizing and bacteriostatic effects, which comprises chitosan oligosaccharide, wherein the chitosan oligosaccharide has good biological safety, broad-spectrum bacteriostatic activity and moisturizing effect, and the dosage of a preservative in the cosmetic is reduced, so that the product has bacteriostatic and anti-inflammatory effects.
3. The invention also provides a preparation method of the cosmetics containing the green alga extract as the active ingredient, and the method has simple and efficient process and is suitable for manufacturing in the cosmetic industry.
Drawings
FIG. 1: example 1 graph of the effect of purified versus unpurified green algae extract on cell proliferation rate;
FIG. 2: example 2 moisture retention test results of mixtures of green algae extract and chitosan oligosaccharide in each ratio;
FIG. 3: example 2 bacteriostatic test result chart of mixture of green algae extract and chitosan oligosaccharide;
FIG. 4: example 3 skin stratum corneum moisture content detection map;
FIG. 5: example 4 skin detection relative value plot;
FIG. 6: example 4 image change of wrinkles and textures of skin.
Detailed Description
The invention adopts the green alga extract from plant sources as an active substance, purifies the active ingredient, prepares the skin care product, adds the chitosan oligosaccharide from animal sources to increase the moisture retention and bacteriostasis effects, and provides a new idea for developing the skin care product with good skin-friendly property, wide sources, obvious effects, nature and safety.
The present invention and its effects will be further described with reference to specific examples, but the embodiments of the invention are not limited thereto.
Example 1: preparation method of green algae extract
Dissolving 1g of green algae powder in hot water at 95 ℃ with the water amount of 100m L, placing the mixed solution in a pressure cooker, heating at 120 ℃ for 40min, extracting, taking out a sample, cooling at room temperature, centrifuging (10000rpm, 30min), removing precipitates after centrifugation, taking supernatant, carrying out vacuum filtration on the supernatant by using a filter membrane with the diameter of 0.2 mu m, and carrying out freeze drying on the filtrate to obtain the green algae extract dry powder.
Dissolving green algae extract powder in distilled water, preparing a solution with the concentration of 5% -10%, adding 2-5 times of volume of absolute ethyl alcohol for alcohol precipitation, standing at 1-8 ℃ for 4-8 h, centrifuging after standing (9000rpm for 30min), collecting precipitate, and freeze-drying the precipitate to obtain the purified green algae extract dry powder.
The purified green algae extract and the green algae extract prepared by the invention are used for carrying out cell proliferation culture experiments, HSF human skin fibroblasts 100 mu L are added into each hole of a 96-hole cell culture plate, wherein the cell density is 1 × 105Placing the powder/m L in a cell incubator for constant temperature and humidity culture for 24h, respectively preparing concentration gradient solutions by PBS (phosphate buffer solution) with the concentrations of 12.5 mu g/m L, 20 mu g/m L, 50 mu g/m L, 100 mu g/m L and 200 mu g/m L, wherein the test 1 group is a purified green algae extract solution with 6 compound wells with the concentration of 10 mu L, the test 2 group is a green algae extract solution with 6 compound wells with the concentration of 10 mu L, the blank control group is 6 compound wells with PBS10 mu L, and after loading, placing a 96-well plate at 37 ℃ and 5% CO2Culturing for 16h in an incubator, taking out the culture plate 4h before the culture is finished, adding 50 mu L MTT solution into each hole, putting the culture plate into a cell culture box, continuously culturing for 4h, sucking out the supernatant, adding 150 mu L DMSO into each hole, uniformly mixing, and detecting the absorbance value of each hole by using a microplate reader, wherein the detection wavelength is 570 nm.
The cell survival rate (mean OD value of extract group/mean OD value of blank group) was × 100%
The results show that both green algae extracts can promote cell proliferation, and the purified green algae extract has a significantly higher effect on cell survival than the non-purified green algae extract (fig. 1).
Example 2: compounding of green alga extract and chitosan oligosaccharide
Chitosan oligosaccharide is mixed with green algae extract.
Mixing the green alga extract prepared by the method with chitosan oligosaccharide according to the mass ratio of 0-1: 1, 1-5: 1, 5-10: 1 and 10-15: 1 to prepare A, B, C, D three mixtures, detecting the moisture retention rate and the bacterial inhibition of the mixtures, weighing 1g of the mixture according to the ratio, respectively dissolving the mixture in 10m L deionized water, placing each group of samples in a low-humidity dryer filled with dry micropowder silica gel, respectively taking out and weighing the samples once after the placement time is 12 hours, 24 hours and 48 hours, and calculating the moisture retention rate of the sample according to the mass difference of the samples before and after the experiment1－m2)/m1×100％，m1The mass of the sample before the experiment is obtained; m is2The mass of the sample after the test.
The results show that the moisture retention rate of each mixture gradually decreases with time, the higher the proportion of the green alga extract in the mixture is, the smaller the moisture retention rate decrease is in 12 hours, after 24 hours, the moisture retention rate decrease of the mixture B is smaller than that of the mixture A, and the moisture retention rate of the mixture C, D has no significant difference (figure 2).
According to the requirements of CB/T7918.1-1987, the general rule of cosmetic microbial standard inspection methods, the A, B, C, D mixture is subjected to bacteriostasis detection. The three kinds of co-test strains are respectively escherichia coli, staphylococcus aureus and bacillus subtilis. Dissolving the three mixtures in deionized water to prepare a solution with the concentration of 1% for testing, wherein the culture medium is a beef extract peptone culture medium, and counting bacterial colonies by a flat plate dilution coating method. Liquid culture medium and strain as control group; let test A, B, C, D for four test groups, each treatment was repeated three times.
The results show that different samples have bacteriostatic effects on escherichia coli, staphylococcus aureus and bacillus subtilis, the mixture has an obvious bacteriostatic effect on escherichia coli, the specific concentration of the component D can generate the bacteriostatic effect on escherichia coli, the bacteriostatic activity of the mixture is reduced along with the reduction of the proportion of oligosaccharides in the mixture, and the mixture of the component B has a good bacteriostatic effect on three bacteria (figure 3).
Example 3: skin test with green algae extract and chitosan oligosaccharide compounded mixture
The method comprises the following specific steps:
1. dissolving 1g of green alga powder in hot water at 95 ℃, wherein the water amount is 100m L, placing the mixed solution in a pressure cooker, heating at 120 ℃ for 40min, extracting, taking out a sample, cooling at room temperature, centrifuging (10000rpm, 30min), removing precipitates after centrifugation, taking supernatant, performing vacuum filtration on the supernatant by using a 0.2 mu m filter membrane, adding 2-5 times of volume of absolute ethyl alcohol into the filtrate for alcohol precipitation, standing at 1-8 ℃ for 4-8 h, centrifuging after standing (9000rpm, 30min), collecting precipitates, and freeze-drying the precipitates to obtain the purified green alga extract dry powder.
2. Mixing the purified green algae extract and chitosan oligosaccharide (molecular weight is 1000 Da-1500 Da) according to the mass ratio of (1-5): 1, and dissolving the mixture in deionized water to prepare a solution with the concentration of 0.01-10%.
3. Selecting 15 volunteers with age of 18-45 years and meeting test requirements as subjects, selecting two symmetrical regions of forearm under stable room temperature environment, respectively as test regions of control group and test group, applying the mixture solution, detecting water content of the two regions with skin detector Courage + Khazaka electronic-MPA10(Corneometer CM825) at intervals of 0.5h, 1h, 2h, 3h, 6h and 12h, and recording the measured value data of each time [ (%) [ (measured value) ÷ (initial value) ] × 100%
4. The results showed that after application of the mixture, the moisture content of the stratum corneum of the skin increased to a maximum value around 2h, and then slowly decreased to 12h, with a moisturizing effect (fig. 4).
Example 4: composite cosmetic using mixture of chlorella extract and chitosan oligosaccharide
1. Dissolving 1g of green alga powder in hot water at 95 ℃, wherein the water amount is 100m L, placing the mixed solution in a pressure cooker, heating at 120 ℃ for 40min, extracting, taking out a sample, cooling at room temperature, centrifuging (10000rpm, 30min), removing precipitates after centrifugation, taking supernatant, performing vacuum filtration on the supernatant by using a 0.2 mu m filter membrane, adding 2-5 times of volume of absolute ethyl alcohol into the filtrate for alcohol precipitation, standing at 1-8 ℃ for 4-8 h, centrifuging after standing (9000rpm, 30min), collecting precipitates, and freeze-drying the precipitates to obtain the purified green alga extract dry powder.
2. The following A, B, C components were formulated
A. Mixing the purified green algae extract with chitosan oligosaccharide (molecular weight is 1000 Da-1500 Da) according to the mass ratio of (1-5): 1, and dissolving the whole components in water according to the mass percentage of 0.1-10% to prepare the active component.
B. 2 to 8 percent of propylene glycol and 5 to 10 percent of glycerol are dissolved in deionized water according to mass percentage to be used as water-soluble components.
C. According to the proportion, 2 to 5 percent of squalane, 2 to 5 percent of stearic acid, 0.5 to 1 percent of whale acetic alcohol, 0.1 to 1 percent of stearyl alcohol, 0.1 to 1 percent of polysorbates, 0.1 to 1 percent of sorbitan stearates, 5 to 10 percent of isopropyl palmitate and 1 to 2 percent of triethanolamine are mixed, stirred evenly and properly heated to be used as an oily component.
3. Heating component C to 80 deg.C, dissolving completely, maintaining at 70 deg.C, heating phase B to 80 deg.C, adding phase B into component C, stirring, emulsifying for 20min, cooling to 50 deg.C, adding component A, homogenizing for 2min, and cooling to room temperature.
Detection of composite cosmetic
And skin quality detection including stratum corneum water content detection, percutaneous water loss detection, skin elasticity detection and image comparison. The apparatus used in this test was CK Coughage + Khazaka electronic-MPA10(Corneometer CM825) in Germany for measuring the moisture in the stratum corneum, Coughage + Khazaka electronic-MPA10(Tewameter TM300) for measuring the moisture loss through the skin, Coughage + Khazaka electronic-MPA10 (Reviometer RVM600) for measuring the elasticity of the skin. Canfield VISTA complex Analysis System images detect wrinkles, texture changes.
The detection method comprises the following steps: 20 volunteers with the age of 18-45 years and meeting the test requirements are selected as the test subjects, and the product of the example is tried for 8 weeks, and is taken in the morning and evening every day, during which no cosmetics except the test products are needed to be smeared. Skin indices were measured at weeks 0, 2, 4, 6, and 8 using the products of the examples, respectively.
Detection area: face part
Data statistics as an index of detection, which is expressed as Mean ± SE (where n is 20), analyzed by a statistical data analysis method, and compared with a relative value (%) (each measured value)/(initial measured value) × 100%.
Detection conditions are as follows: the indoor environment is stable, the temperature is 20 +/-2 ℃, and the ambient humidity is 40-60%.
And (3) detection results: the results show that the water content of the skin stratum corneum, the skin elasticity and the percutaneous water loss are obviously changed from 2 weeks, the water content of the stratum corneum is increased by 15.2%, and the water content of the stratum corneum is increased by 19.5% by the end of the experiment at 8 weeks; the skin elasticity is increased by 6.6% from 2.1% in the second week to 8 weeks, and the skin elasticity is obviously increased; the transdermal water dispersion gradually decreased, which was closely related to the improvement of skin texture, and the relative value of transdermal water dispersion decreased by 7.3% at week 8 (fig. 5). The results of skin imaging showed that image contrast revealed a reduction in skin texture, a reduction in wrinkles, and a sustained improvement in skin texture (fig. 6).
Claims (7)
1. The green alga extract is characterized in that the preparation method of the green alga extract comprises the following steps:
dissolving green algae powder in hot water, and extracting at high temperature; centrifuging the extracted sample and taking supernatant; filtering the supernatant with a filter membrane, and freeze-drying the filtrate to obtain dry extract powder; dissolving the dry powder, and precipitating with absolute ethyl alcohol; freeze drying the precipitate to obtain Chlorella extract.
2. Use of the green algae extract of claim 1 in the preparation of a moisturizing, antimicrobial product.
3. A moisturizing and antibacterial product, which is characterized in that the raw materials of the product are the green alga extract and the chitosan oligosaccharide of claim 1.
4. The product of claim 3, wherein the mass ratio of the green algae extract to the chitosan oligosaccharide is 1-15: 1.
5. The product of claim 3, wherein the mass ratio of the green algae extract to the chitosan oligosaccharide is 1-5: 1.
6. The preparation according to any one of claims 3 to 5, wherein components commonly used in the cosmetic field are further added to the preparation.
7. The product of claim 6, wherein the component comprises any one or more of propylene glycol, glycerin, squalane, stearic acid, cetyl alcohol, stearyl alcohol, polysorbate, sorbitan stearate, isopropyl palmitate, and triethanolamine.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113786358A (en) * | 2021-09-02 | 2021-12-14 | 科盈生物科技(惠州)有限公司 | Green alga scalp-soothing nursing agent |
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| CN113786358A (en) * | 2021-09-02 | 2021-12-14 | 科盈生物科技(惠州)有限公司 | Green alga scalp-soothing nursing agent |
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