CN109042081A - A method of bacterium material is discarded as the wild black fungus of main substrate culture with Rhizoma Gastrodiae - Google Patents
A method of bacterium material is discarded as the wild black fungus of main substrate culture with Rhizoma Gastrodiae Download PDFInfo
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- CN109042081A CN109042081A CN201811221608.1A CN201811221608A CN109042081A CN 109042081 A CN109042081 A CN 109042081A CN 201811221608 A CN201811221608 A CN 201811221608A CN 109042081 A CN109042081 A CN 109042081A
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- rhizoma gastrodiae
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The present invention discloses a kind of method for discarding bacterium material with Rhizoma Gastrodiae as the wild black fungus of main substrate culture, comprising: Rhizoma Gastrodiae discards bacterium material, wheat bran, soybean powder, land plaster, KH2PO4And MgSO4.This discards the method that bacterium material is the wild black fungus of main substrate culture with Rhizoma Gastrodiae, using discarded bacterium material remaining during gastrodin cultivation as main material, carry out the application of wild black fungus cultivating in bag, it realizes Rhizoma Gastrodiae and discards bacterium material recycling in the production of wild cultivation of auricularia auricula, improve the utilization rate of forest tree resource in agricultural production, it opens Rhizoma Gastrodiae and discards the new channel that bacterium material is applied in the cultivation of wild black fungus bag material, reduce the investment of traditional compost, save a large amount of raw material, and existing market does not occur also discarding bacterium material as main matrix using Rhizoma Gastrodiae, carry out the report of wild cultivation of auricularia auricula production.
Description
Technical field
The invention belongs to cultivation of auricularia auricula technical fields, more particularly to one kind to discard bacterium material with Rhizoma Gastrodiae as main substrate culture
The method of wild black fungus.
Background technique
During Rhizoma Gastrodiae plantation, the cultivation cultivated from Armillaria mellea culture, rice fiber crops to sisal hemp be unable to do without bacterium material all for honey
Ring bacterium provides nutrition, so not only needing to expend a large amount of timber resources during annual gastrodin cultivation, while will also generate big
The bacterium material waste of amount plants the discarded bacterium material of Rhizoma Gastrodiae at present, and just abandoned is in forest land after Rhizoma Gastrodiae excavation mostly, shortage pair
Rhizoma Gastrodiae discards bacterium material and further recycles and develop, and there are also more nutriments for most of bacterium material, arbitrarily abandons, causes
The waste of timber resources is unfavorable for the raising of forest tree resource utilization rate in agricultural production.
Traditional culture materials of edible fungi is mostly used weedtree, weed tree sawdust, cotton seed hulls etc., with the reinforcement of forest conservation, wood
The cutting capacity of material is reduced increasingly, and the sense of weed tree sawdust raw material day is insufficient, though cotton seed hulls is good, the place of production is mostly in northern China, southern province
Not only price is purchased, but also is difficult to buy.
Summary of the invention
The present invention, which is provided, discards the method that bacterium material is the wild black fungus of main substrate culture with Rhizoma Gastrodiae, it is intended to solve traditional
Culture materials of edible fungi is mostly used weedtree, weed tree sawdust, cotton seed hulls etc., and with the reinforcement of forest conservation, the cutting capacity of timber is increasingly
It reducing, the sense of weed tree sawdust raw material day is insufficient, though cotton seed hulls is good, mostly in northern China, not only price is purchased in southern province in the place of production,
And it is difficult to the problem of buying.
The invention is realized in this way a method of with Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture,
It include: that Rhizoma Gastrodiae discards bacterium material, wheat bran, soybean powder, land plaster, KH2PO4And MgSO4。
Preferably, the quality mixture ratio between each component are as follows:
Preferably, it is described with Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) it takes Rhizoma Gastrodiae to discard bacterium material, after removing the discarded bacterium material surface soil of Rhizoma Gastrodiae, diameter is crushed to after drying less than 0.5cm
Sawdust, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus after tissue isolates and purifies
Strain, black fungus strain are inoculated in fluid nutrient medium, are kept for 25 DEG C of temperature, standby using shaking table with 120r/min culture one week
With；
3) take step 1) give over to spare Rhizoma Gastrodiae discard bacterium material, according to quality proportioning be added wheat bran, soybean powder, land plaster,
KH2PO4And MgSO4, after being mixed, tap water mixing is added by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, by the base of mixing
Matter is fitted into bacterium bag, and be locked fruiting ring, and high-temperature sterilization is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml in bacterium bag fruiting ring
End carries out the inoculation of black fungus strain, and the bacterium bag after inoculation is protected from light bacteria under the conditions of 25 DEG C, gives over to spare；
5) step 4) is taken to give over to spare matrix, when covering with wild black fungus mycelia in matrix, by bacterium bag surface sterilization,
Bacterium bag is open with " V " type using blade, is placed in the hayashishita that canopy density are greater than 70%, guarantees to be gone out between humidity 75-85%
Mushroom catalysis.
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out within 3-4 days agaric fructification picking, fructification biology
Conversion ratio 30-35%.
Preferably, the Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang.
Preferably, the fluid nutrient medium is 150ml/ bottles, and 10 pieces of black fungus bacterials are inoculated with inside every bottle of fluid nutrient medium
Kind.
Preferably, the specification of the bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix.
Compared with prior art, the beneficial effects of the present invention are:
1, using discarded bacterium material remaining during gastrodin cultivation as main material, carry out answering for wild black fungus cultivating in bag
With, realize Rhizoma Gastrodiae discard bacterium material wild cultivation of auricularia auricula production in recycling, improve forest tree resource in agricultural production
In utilization rate, open Rhizoma Gastrodiae and discard bacterium material new channel for applying in the cultivation of wild black fungus bag material.
2, bacterium material is discarded using Rhizoma Gastrodiae and replace traditional weedtree and cotton seed hulls, not only increase and the secondary of bacterium material is discarded to Rhizoma Gastrodiae
It utilizes, and reduces the investment of traditional compost, save a large amount of raw material, it is multi-purpose to solve traditional culture materials of edible fungi
Weedtree, weed tree sawdust, cotton seed hulls etc., with the reinforcement of forest conservation, the cutting capacity of timber is reduced increasingly, weed tree sawdust raw material day
Sense is insufficient, though cotton seed hulls is good, the place of production is mostly in northern China, and not only price is purchased in southern province, but also be difficult to buy asks
Topic.
3, cultivation strain is wild black fungus strain, and the product after cultivating in bag can greatly remain wild black wood
Character and mouthfeel, can meet in the market consumer welcome.
Detailed description of the invention
Fig. 1 is system flow chart of the invention；
Fig. 2 is the wild black fungus fruiting figure that the cultivation of bacterium material is discarded using Rhizoma Gastrodiae.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.
In the description of the present invention, it is to be understood that, term " length ", " width ", "upper", "lower", "front", "rear",
The orientation or positional relationship of the instructions such as "left", "right", "vertical", "horizontal", "top", "bottom" "inner", "outside" is based on attached drawing institute
The orientation or positional relationship shown, is merely for convenience of description of the present invention and simplification of the description, rather than the dress of indication or suggestion meaning
It sets or element must have a particular orientation, be constructed and operated in a specific orientation, therefore should not be understood as to limit of the invention
System.In addition, in the description of the present invention, the meaning of " plurality " is two or more, unless otherwise specifically defined.
The present invention provides a kind of technical solution: a kind of to discard the side that bacterium material is the wild black fungus of main substrate culture with Rhizoma Gastrodiae
Method, comprising: Rhizoma Gastrodiae discards bacterium material, wheat bran, soybean powder, land plaster, KH2PO4And MgSO4。
Quality mixture ratio between each component are as follows:
With Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) Rhizoma Gastrodiae is taken to discard bacterium material, Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang, removes Rhizoma Gastrodiae and discards
After the soil of bacterium material surface, the sawdust that diameter is less than 0.5cm is crushed to after drying, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus after tissue isolates and purifies
Strain, black fungus strain are inoculated in fluid nutrient medium, and fluid nutrient medium is 150ml/ bottles, and is connect inside every bottle of fluid nutrient medium
10 pieces of black fungus strains of kind, are kept for 25 DEG C of temperature, spare using shaking table with 120r/min culture one week；
3) take step 1) give over to spare Rhizoma Gastrodiae discard bacterium material, according to quality proportioning be added wheat bran, soybean powder, land plaster,
KH2PO4And MgSO4, after being mixed, tap water mixing is added by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, by the base of mixing
Matter is fitted into bacterium bag, and the specification of bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix, and be locked fruiting ring, high-temperature sterilization,
It is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml in bacterium bag fruiting ring
End carries out the inoculation of black fungus strain, and the bacterium bag after inoculation is protected from light bacteria under the conditions of 25 DEG C, gives over to spare；
5) step 4) is taken to give over to spare matrix, when covering with wild black fungus mycelia in matrix, by bacterium bag surface sterilization,
Bacterium bag is open with " V " type using blade, is placed in the hayashishita that canopy density are greater than 70%, guarantees to be gone out between humidity 75-85%
Mushroom catalysis.
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out within 3-4 days agaric fructification picking, fructification biology
Conversion ratio 30-35%.
In the present invention, using discarded bacterium material remaining during gastrodin cultivation as main material, carry out wild black fungus Bag Material
The application of cultivation realizes Rhizoma Gastrodiae and discards recycling of the bacterium material in the production of wild cultivation of auricularia auricula, improves forest tree resource and exist
Utilization rate in agricultural production opens Rhizoma Gastrodiae and discards the new channel that bacterium material is applied in the cultivation of wild black fungus bag material, reduces
The investment of traditional compost, saves a large amount of raw material, solves traditional culture materials of edible fungi and is mostly used weedtree, weedtree
Bits, cotton seed hulls etc., with the reinforcement of forest conservation, the cutting capacity of timber is reduced increasingly, and the sense of weed tree sawdust raw material day is insufficient, cotton
Though seed shell is good, mostly in northern China, not only price is purchased in southern province, but also is difficult to the problem of buying in the place of production.
It is clearly and completely described below in conjunction with the technical solution in the embodiment of the present invention, it is clear that described reality
Applying example is only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without making creative work belongs to what the present invention protected
Range.
Embodiment one
A method of bacterium material being discarded as the wild black fungus of main substrate culture with Rhizoma Gastrodiae, comprising: Rhizoma Gastrodiae discards bacterium material, wheat
Bran, soybean powder, land plaster, KH2PO4And MgSO4。
Quality mixture ratio between each component are as follows:
With Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) Rhizoma Gastrodiae is taken to discard bacterium material, Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang, removes Rhizoma Gastrodiae and discards
After the soil of bacterium material surface, the sawdust that diameter is less than 0.5cm is crushed to after drying, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus after tissue isolates and purifies
Strain, black fungus strain are inoculated in fluid nutrient medium, and fluid nutrient medium is 150ml/ bottles, and is connect inside every bottle of fluid nutrient medium
10 pieces of black fungus strains of kind, are kept for 25 DEG C of temperature, spare using shaking table with 120r/min culture one week；
3) take step 1) give over to spare Rhizoma Gastrodiae discard bacterium material, according to quality proportioning be added wheat bran, soybean powder, land plaster,
KH2PO4And MgSO4, after being mixed, tap water mixing is added by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, by the base of mixing
Matter is fitted into bacterium bag, and the specification of bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix, and be locked fruiting ring, high-temperature sterilization,
It is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml in bacterium bag fruiting ring
End carries out the inoculation of black fungus strain, and the bacterium bag after inoculation is protected from light bacteria under the conditions of 25 DEG C, gives over to spare；
5) step 4) is taken to give over to spare matrix, when covering with wild black fungus mycelia in matrix, by bacterium bag surface sterilization,
Bacterium bag is open with " V " type using blade, is placed in the hayashishita that canopy density are greater than 70%, guarantees to be gone out between humidity 75-85%
Mushroom catalysis.
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out within 3-4 days agaric fructification picking, fructification biology
Conversion ratio 30-35%.
In the present embodiment, land plaster can directly in supplementing culture medium sulphur, calcium deficiency, black fungus is provided and grows each stage pair
Calcium ion demand, while the addition of land plaster can adjust the PH environment in bacterium bag, prevent pH value in matrix too low, influence to cultivate
The influence that agaric mycelium is grown and is broken up in the process.
Embodiment two
A method of bacterium material being discarded as the wild black fungus of main substrate culture with Rhizoma Gastrodiae, comprising: Rhizoma Gastrodiae discards bacterium material, wheat
Bran, soybean powder, land plaster, KH2PO4And MgSO4。
Quality mixture ratio between each component are as follows:
With Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) Rhizoma Gastrodiae is taken to discard bacterium material, Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang, removes Rhizoma Gastrodiae and discards
After the soil of bacterium material surface, the sawdust that diameter is less than 0.5cm is crushed to after drying, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus after tissue isolates and purifies
Strain, black fungus strain are inoculated in fluid nutrient medium, and fluid nutrient medium is 150ml/ bottles, and is connect inside every bottle of fluid nutrient medium
10 pieces of black fungus strains of kind, are kept for 25 DEG C of temperature, spare using shaking table with 120r/min culture one week；
3) take step 1) give over to spare Rhizoma Gastrodiae discard bacterium material, according to quality proportioning be added wheat bran, soybean powder, land plaster,
KH2PO4And MgSO4, after being mixed, tap water mixing is added by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, by the base of mixing
Matter is fitted into bacterium bag, and the specification of bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix, and be locked fruiting ring, high-temperature sterilization,
It is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml in bacterium bag fruiting ring
End carries out the inoculation of black fungus strain, and the bacterium bag after inoculation is protected from light bacteria under the conditions of 25 DEG C, gives over to spare；
5) step 4) is taken to give over to spare matrix, when covering with wild black fungus mycelia in matrix, by bacterium bag surface sterilization,
Bacterium bag is open with " V " type using blade, is placed in the hayashishita that canopy density are greater than 70%, guarantees to be gone out between humidity 75-85%
Mushroom catalysis.
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out within 3-4 days agaric fructification picking, fructification biology
Conversion ratio 30-35%.
In the present embodiment, a large amount of protein, fat and microelement are contained in soybean powder, be it is a kind of preferably add it is auxiliary
Material, develops demand of each stage to nutriment so as to supplement growth.
Embodiment three
A method of bacterium material being discarded as the wild black fungus of main substrate culture with Rhizoma Gastrodiae, comprising: Rhizoma Gastrodiae discards bacterium material, wheat
Bran, soybean powder, land plaster, KH2PO4And MgSO4。
Quality mixture ratio between each component are as follows:
With Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) Rhizoma Gastrodiae is taken to discard bacterium material, Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang, removes Rhizoma Gastrodiae and discards
After the soil of bacterium material surface, the sawdust that diameter is less than 0.5cm is crushed to after drying, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus after tissue isolates and purifies
Strain, black fungus strain are inoculated in fluid nutrient medium, and fluid nutrient medium is 150ml/ bottles, and is connect inside every bottle of fluid nutrient medium
10 pieces of black fungus strains of kind, are kept for 25 DEG C of temperature, spare using shaking table with 120r/min culture one week；
3) take step 1) give over to spare Rhizoma Gastrodiae discard bacterium material, according to quality proportioning be added wheat bran, soybean powder, land plaster,
KH2PO4And MgSO4, after being mixed, tap water mixing is added by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, by the base of mixing
Matter is fitted into bacterium bag, and the specification of bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix, and be locked fruiting ring, high-temperature sterilization,
It is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml in bacterium bag fruiting ring
End carries out the inoculation of black fungus strain, and the bacterium bag after inoculation is protected from light bacteria under the conditions of 25 DEG C, gives over to spare；
5) step 4) is taken to give over to spare matrix, when covering with wild black fungus mycelia in matrix, by bacterium bag surface sterilization,
Bacterium bag is open with " V " type using blade, is placed in the hayashishita that canopy density are greater than 70%, guarantees to be gone out between humidity 75-85%
Mushroom catalysis.
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out within 3-4 days agaric fructification picking, fructification biology
Conversion ratio 30-35%.
In the present embodiment, KH2PO4Addition, supplemented with black fungus in different stages of growth to potassium ion and phosphate anion
Demand, there is significant increasing both production and income, the amount of changing optimization quality, disease and insect resistance, many excellent effects of prevention and treatment early ageing etc., so as to
The yield of black fungus during raising cultivating in bag.
Example IV
A method of bacterium material being discarded as the wild black fungus of main substrate culture with Rhizoma Gastrodiae, comprising: Rhizoma Gastrodiae discards bacterium material, wheat
Bran, soybean powder, land plaster, KH2PO4And MgSO4。
Quality mixture ratio between each component are as follows:
With Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) Rhizoma Gastrodiae is taken to discard bacterium material, Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang, removes Rhizoma Gastrodiae and discards
After the soil of bacterium material surface, the sawdust that diameter is less than 0.5cm is crushed to after drying, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus after tissue isolates and purifies
Strain, black fungus strain are inoculated in fluid nutrient medium, and fluid nutrient medium is 150ml/ bottles, and is connect inside every bottle of fluid nutrient medium
10 pieces of black fungus strains of kind, are kept for 25 DEG C of temperature, spare using shaking table with 120r/min culture one week；
3) take step 1) give over to spare Rhizoma Gastrodiae discard bacterium material, according to quality proportioning be added wheat bran, soybean powder, land plaster,
KH2PO4 and MgSO4 after being mixed, is added tap water mixing by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, by the base of mixing
Matter is fitted into bacterium bag, and the specification of bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix, and be locked fruiting ring, high-temperature sterilization,
It is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml in bacterium bag fruiting ring
End carries out the inoculation of black fungus strain, and the bacterium bag after inoculation is protected from light bacteria under the conditions of 25 DEG C, gives over to spare；
5) step 4) is taken to give over to spare matrix, when covering with wild black fungus mycelia in matrix, by bacterium bag surface sterilization,
Bacterium bag is open with " V " type using blade, is placed in the hayashishita that canopy density are greater than 70%, guarantees to be gone out between humidity 75-85%
Mushroom catalysis.
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out within 3-4 days agaric fructification picking, fructification biology
Conversion ratio 30-35%.
In the present embodiment, MgSO4Magnesium elements can be provided for matrix, and relatively commonly mend magnesium fertilizer, MgSO4Dissolubility it is more preferable,
So as to keep benefit magnesium effect more preferable.
The above is merely preferred embodiments of the present invention, be not intended to limit the invention, it is all in spirit of the invention and
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within principle.
Claims (6)
1. a kind of discard the method that bacterium material is the wild black fungus of main substrate culture with Rhizoma Gastrodiae characterized by comprising Rhizoma Gastrodiae is useless
Abandon bacterium material, wheat bran, soybean powder, land plaster, KH2PO4And MgSO4。
2. according to claim 1 discard the method that bacterium material is the wild black fungus of main substrate culture, feature with Rhizoma Gastrodiae
It is: the quality mixture ratio between each component are as follows:
3. according to claim 2 discard the method that bacterium material is the wild black fungus of main substrate culture, feature with Rhizoma Gastrodiae
Be: it is described with Rhizoma Gastrodiae discard bacterium material be the wild black fungus of main substrate culture method the following steps are included:
1) it takes Rhizoma Gastrodiae to discard bacterium material, after removing the discarded bacterium material surface soil of Rhizoma Gastrodiae, dries and be crushed to the wood that diameter is less than 0.5cm
Bits, it is spare；
2) it takes field grown in the wild black fungus fructification of water wax gourd rotten wood, obtains black fungus bacterial after tissue isolates and purifies
Kind, black fungus strain is inoculated in fluid nutrient medium, is kept for 25 DEG C of temperature, spare using shaking table with 120r/min culture one week；
3) it takes step 1) to give over to spare Rhizoma Gastrodiae and discards bacterium material, wheat bran, soybean powder, land plaster, KH is added according to quality proportioning2PO4
And MgSO4, after being mixed, tap water mixing is added by material water quality ratio 1:2 and mixes thoroughly to obtain matrix, the matrix of mixing is packed into
In bacterium bag, be locked fruiting ring, and high-temperature sterilization is spare；
4) step 3) is taken to give over to spare bacterium bag, aseptically according to the inoculum concentration of every bag of 30ml bacterium bag fruiting ring end into
The inoculation of row black fungus strain, the bacterium bag after inoculation are protected from light bacteria under the conditions of 25 DEG C, give over to spare；
5) it takes step 4) to give over to spare matrix, after agaric mycelium covers with bacterium bag, by bacterium bag surface sterilization, uses blade
Bacterium bag is open with " V " type, is placed in the hayashishita that canopy density are greater than 70%, guarantees to carry out fruiting catalysis between humidity 75-85%；
6) after bacterium bag opening, 8-10 days former bases start to break up, and can carry out agaric fructification picking, fructification bioconversion within 3-4 days
Rate 30-35%.
4. according to claim 1 discard the method that bacterium material is the wild black fungus of main substrate culture, feature with Rhizoma Gastrodiae
Be: the Rhizoma Gastrodiae discards bacterium material mainly with Fagaceae, based on the tree of Qinggang.
5. according to claim 3 discard the method that bacterium material is the wild black fungus of main substrate culture, feature with Rhizoma Gastrodiae
Be: the fluid nutrient medium is 150ml/ bottles, and 10 pieces of black fungus strains are inoculated with inside every bottle of fluid nutrient medium.
6. according to claim 1 discard the method that bacterium material is the wild black fungus of main substrate culture, feature with Rhizoma Gastrodiae
Be: the specification of the bacterium bag is 8 × 30cm, and every bag of bacterium bag is packed into 300g matrix.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109717000A (en) * | 2019-02-18 | 2019-05-07 | 铜仁职业技术学院 | A method of oyster mushroom is cultivated as raw material using day anesthesia stem |
| CN117337723A (en) * | 2023-11-24 | 2024-01-05 | 西南林业大学 | Gastrodia elata cultivation medium and preparation method and application thereof |
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| CN109717000B (en) * | 2019-02-18 | 2021-06-11 | 铜仁职业技术学院 | Method for cultivating oyster mushrooms by taking gastrodia elata stalks as raw materials |
| CN117337723A (en) * | 2023-11-24 | 2024-01-05 | 西南林业大学 | Gastrodia elata cultivation medium and preparation method and application thereof |
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