CN108996705A - Method for promoting forage-available fungi to convert water eutrophication substances by using carbon source and application of obtained product - Google Patents
Method for promoting forage-available fungi to convert water eutrophication substances by using carbon source and application of obtained product Download PDFInfo
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 52
- 241000233866 Fungi Species 0.000 title claims abstract description 33
- 238000012851 eutrophication Methods 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 28
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 229910052799 carbon Inorganic materials 0.000 title claims abstract description 26
- 239000000126 substance Substances 0.000 title claims abstract description 18
- 230000001737 promoting effect Effects 0.000 title abstract 2
- 239000008188 pellet Substances 0.000 claims abstract description 38
- 241001465754 Metazoa Species 0.000 claims abstract description 25
- 239000000463 material Substances 0.000 claims description 13
- 230000009466 transformation Effects 0.000 claims description 9
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 8
- 230000002538 fungal effect Effects 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 229910052698 phosphorus Inorganic materials 0.000 claims description 8
- 239000011574 phosphorus Substances 0.000 claims description 8
- 238000005276 aerator Methods 0.000 claims description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 4
- 239000005416 organic matter Substances 0.000 claims description 4
- 239000001301 oxygen Substances 0.000 claims description 4
- 229910052760 oxygen Inorganic materials 0.000 claims description 4
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 239000000470 constituent Substances 0.000 claims description 2
- 150000002016 disaccharides Chemical class 0.000 claims description 2
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims description 2
- 235000013379 molasses Nutrition 0.000 claims description 2
- 150000002772 monosaccharides Chemical class 0.000 claims description 2
- 229940095674 pellet product Drugs 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- -1 small molecule carbohydrate Chemical class 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 239000005720 sucrose Substances 0.000 claims description 2
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims 1
- 238000009630 liquid culture Methods 0.000 claims 1
- 150000004676 glycans Chemical class 0.000 abstract description 13
- 229920001282 polysaccharide Polymers 0.000 abstract description 13
- 239000005017 polysaccharide Substances 0.000 abstract description 13
- 102000004169 proteins and genes Human genes 0.000 abstract description 7
- 108090000623 proteins and genes Proteins 0.000 abstract description 7
- 230000000975 bioactive effect Effects 0.000 abstract description 4
- 239000003344 environmental pollutant Substances 0.000 abstract description 4
- 231100000719 pollutant Toxicity 0.000 abstract description 4
- 238000000746 purification Methods 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 244000052616 bacterial pathogen Species 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 22
- 229910052757 nitrogen Inorganic materials 0.000 description 10
- 230000000694 effects Effects 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 241001264174 Cordyceps militaris Species 0.000 description 4
- GQPLMRYTRLFLPF-UHFFFAOYSA-N Nitrous Oxide Chemical compound [O-][N+]#N GQPLMRYTRLFLPF-UHFFFAOYSA-N 0.000 description 4
- 241001248610 Ophiocordyceps sinensis Species 0.000 description 4
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 4
- 238000002798 spectrophotometry method Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000005189 flocculation Methods 0.000 description 2
- 230000016615 flocculation Effects 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000001272 nitrous oxide Substances 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 239000003403 water pollutant Substances 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- VAYOSLLFUXYJDT-RDTXWAMCSA-N Lysergic acid diethylamide Chemical compound C1=CC(C=2[C@H](N(C)C[C@@H](C=2)C(=O)N(CC)CC)C2)=C3C2=CNC3=C1 VAYOSLLFUXYJDT-RDTXWAMCSA-N 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 description 1
- 241000605154 Nitrobacter winogradskyi Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 102100022419 RPA-interacting protein Human genes 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000005067 remediation Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000015067 sauces Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a method for promoting forage-able fungi to convert water eutrophication substances by using a carbon source and application of obtained products, wherein the conversion method comprises the following steps: (1) a water body containing eutrophication substances is filled in the bioreactor, and a carbon source is added; (2) inoculating a feeding fungus into the bioreactor, and performing intermittent culture for 1-3 days at room temperature or continuous culture to assimilate eutrophic substances into newly-proliferated mycelium pellets; (3) preparing animal feed by using the newly proliferated mycelium pellet. The product is rich in protein, polysaccharide and other bioactive components, and can promote animal growth, inhibit pathogenic bacteria and ensure animal health. On one hand, the invention reuses eutrophication substances in the water body, reduces the pollutant discharge and realizes the water environment purification; on the other hand, the fungus mycelium product which is rich in nutrition and has the health care function on animals is obtained. Not only protects the water environment, but also obtains high-quality animal feed, and has wide application value.
Description
Technical field
The present invention relates to it is a kind of with carbon source promote can feeding fungal transformation water eutrophication substance method and gained produce
The application of object, particular by addition carbon source promote can feeding fungal transformation water eutrophication substance obtain mycoprotein produce
Object belongs to environmental protection and resource circulation utilization field.
Background technique
Water eutrophication has become the global problem of environmental pollution being concerned and is investigated according to statistics in Europe
In 96 lakes, reservoir, there is 80% pollution by nitrogen, phosphorus, eutrophic state is presented.Chinese eutrophication and weight eutrophy
Change lake and also reach lake total amount 66% and 22%, wherein the also scapes such as the five big fresh water lakes including Chinese Famous and the tourist attractions West Lake
See water body.Total nitrogen and total phosphorus concentration are up to 20mg/L and 1.5mg/L respectively.
Microorganism remediation technology plays an important role in water body purification, because it is easy to use, do not need increase project amount
The advantages that preferable with treatment effect, become one of research hotspot of environmental area.The technology utilizes microorganism decomposition metabolism
It realizes to water pollutant transfer, conversion and degradation, purifying water body.For example, passing through denitrification for ammonia nitrogen and nitrite
Etc. being converted into nitrogen and nitrous oxide etc..The catabolism of bacterium nitrobacter is utilized in Dai Zhidong (CN201610294795.0),
Realize the purification of breeding water body.But the process can consume a large amount of carbon source and release GHG carbon dioxide, and an oxygen
Changing the metabolites such as phenodiazine may cause serious atmosphere pollution.
Biological flocculation technology is the anabolic action by microorganism, by the carbon source of proper proportion, assimilation water
Nutrient pollutant in body forms mycoprotein that can be feeding by cultivated animals, and reduces water pollutant concentration.However,
Biological flocculation microbe species rich in will realize that effective manual control is very difficult, therefore its complicated component
It is changeable, it is unfavorable for recycling.
Fungi is unicellular or many cells heterotrophism eukaryotic microorganisms, it can be common that all kinds of Mushrooms (macro fungi) also include
Mould and yeast, it is most beneficial to the mankind, such as sauce processed, wine brewing, food processing, fermented feed and environmental protection.For example,
White-rot fungi and other microorganisms are mixed into feed by Dai Zhidong (CN201610294795.0), and fermentation production of protein matter is
Aquatic livestock provides existence resource.Immobilizing white rot fungi is utilized except algae control algae in Wang Pu etc. (CN201510382860.0), goes
Except the chlorophyll pollutant in water body.Wherein, fungi polysaccharide is the important high-molecular compound of one kind as caused by fungi,
It is safe and non-toxic, physicochemical property is unique, the effects of having various bioactivity, cell and immunity of organism can be enhanced.The egg of fungi
White and polypeptide classes are abundant, have the bioactivity such as RIP activity, antiviral and immunological regulation.Due to fungi polysaccharide and albumen
It is with short production cycle, not by season and territory restriction, there is the stronger market competitiveness and vast potential for future development.It is raiseeed in the modern times
Application practice in animal husbandry proves, fungi polysaccharide and protein extract can be obviously promoted the raising of Production of Livestock and Poultry ability.
Summary of the invention
It can feeding fungal transformation water eutrophication compound with carbon source promotion in view of this, the purpose of the present invention is to propose to one kind
The method of matter and the application of products therefrom, be by additional carbon strengthen can feeding fungi assimilation, remove water body in
Eutrophication materials.On the one hand Eutrophication materials in water body are recycled, reduces pollutant emission, it is net to realize water environment
Change；On the other hand fungal mycelium product that is full of nutrition and having healthcare function to animal is obtained.Not only water environment is protected, but also
High-quality animal feed is obtained, is with a wide range of applications.
In order to solve the above technical problems, the present invention is achieved by the following scheme:
A method of with carbon source promote can feeding fungal transformation water eutrophication substance, include the following steps:
(1) it is packed into the water body containing Eutrophication materials in the bioreactor, while adding carbon source；
(2) into bioreactor inoculation can feeding fungi mycelium pellet or mycelia block, 1~3d of occasional drive at room temperature
Or continuous culture, so that Eutrophication materials is assimilated into the component part for the mycelium pellet being newly proliferated, such as mycoprotein and polysaccharide and its
Its bioactive substance；
(3) it collects the mycelium pellet being newly proliferated and is used to prepare animal feed.
Further, in step of the invention (1), the bioreactor includes triangular flask (200mL~2L), fermentor
(20L~1000L) or reactive tank (1m3~1000m3) and other culture vessels of various shapes, in addition, bioreactor configurations
There are stirring or aerator.The mixing speed of agitating device is 20~200 revs/min, and aerator reaches dissolved oxygen in water body
To 2~8mg/L.
Further, in step of the invention (1), Eutrophication materials include in the water body containing Eutrophication materials
Ammonia nitrogen, nitrate nitrogen and itrogenous organic substance and phosphate and organic phosphorus, the Water constituent content containing Eutrophication materials are 2
~1000mg N/L, phosphorus element content are 0.1~100mg P/L.
Further, in step of the invention (1), the carbon source is glucose, sucrose, malt extract and molasses and other
It can be analyzed to the processing product of monosaccharide, the natural organic matter of disaccharides and small molecule carbohydrate or natural organic matter, the carbon source amount of addition
It is equivalent to 0.02~20g/L of glucose.
Further, in step of the invention (2), it is described can feeding fungi include filamentous fungi and macro fungi, preferably
For entomogenous fungi, the fungi is on the one hand big to the demand of nitrogen source, on the other hand can produce mycoprotein and polysaccharide and other right
Cultivated animals have the substance of health-care effect.
Further, in step of the invention (2), the mycelium pellet is the mycelia that fungi is formed in liquid medium
Ball；The mycelia block is the mycelia block of fungi picking on solid culture primary surface.Mycelium pellet or mycelia block with 0.4%~
5% inoculum concentration accesses in corresponding triangular flask, fermentor or reactive tank class bioreactor.
Further, in step of the invention (2), the occasional drive refers to that bioreactor is to fill a water, row one
Secondary water so repeats；The continuous culture refers to that bioreactor is constantly water inlet and constantly draining.
Further, in step of the invention (3), the mycelium pellet of the new proliferation using 0.2~2mm of aperture gauze or
Filter bag is collected, and the new mycelium pellet yield that is proliferated is 10~500g/L.
It is a kind of to promote mycelium pellet product to exist made from the method for feeding fungal transformation water eutrophication substance with carbon source
Application on animal feed, including cultivated animals are directly fed with newly-increased mycelium pellet, or after newly-increased mycelium pellet is mixed into feed
Cultivated animals are fed again.
Technical principle of the invention are as follows: under conditions of carbon source abundance, fungi largely inhales from water body by anabolism
The nutrients such as nitrogen phosphorus are received, realizes that itself fast breeding is grown, forms mycoprotein and polysaccharide and other bioactive substances, reach
To the purpose of Eutrophication materials resource utilization.The anabolism be convert mycoprotein for ammonia nitrogen and nitrate nitrogen etc., and
It is not to be converted into nitrogen and nitrous oxide, and the carbon source supplemented is converted into fungi polysaccharide.
Thick many candies and thick protein in mycelium pellet are isolated with common hot water extraction method, with common phenolsulfuric acid
It is 2%~12% that method, which measures its so many sugared content, measures mycoprotein content with Coomassie Brilliant Blue as 2%~10%.Using
The remaining total nitrogen concentration that national standard method alkaline chitinase resolution ultraviolet spectrophotometry measures water phase is 0.5~20mg/L, right
The nitrogen removal rate answered is 60%~90%.
Compared with prior art, the positive effect of the present invention is:
(1) the mycelium pellet yield prepared by is big, and mycoprotein and polyoses content are high in the mycelium pellet being newly proliferated.
(2) nitrogen phosphor resource valuable in water body " is exchanged " for carbon source cheap and easy to get in the present invention, both obtained valuable
Protein and the high value added products such as polysaccharide and other bioactive substances, and can be reduced nitrogen phosphorus bring eutrophication and ask
Topic has both and has an economic benefit and environmental benefit.
(3) the feeding fungi in the present invention contains the substance for largely having health-care effect to cultivated animals, is highly suitable as
Animal feed uses.
(4) nutrient transformation efficiency in this method is high, easy to operate, and ratio for input and output is high, and general field condition can be into
Row, thus it is easy to spread.
Specific embodiment
The present invention is got information about to allow those skilled in the art to be more clear, the present invention will be made below further
Explanation.
Embodiment 1
(1) the eutrophication water sample 400mL of human configuration is packed into 1L triangular flask, wherein containing ammonium sulfate 135mg/L
(110mg N/L).Glucose is added simultaneously to 550mg/L, and compares group with do not add glucose.
(2) into triangular flask be inoculated with Cordyceps Militaris mycelium pellet suspension 20mL, mycelium pellet weight in wet base be 4g, on shaking table in
160 revs/min, 28 DEG C of occasional drive 3d, during which carbon source and ammonium sulfate etc. is constantly absorbed and utilized in the mycelium pellet from water phase, generates
New mycelium pellet；Simultaneously it has been observed that due to a lack of carbon source, the not new mycelium pellet of control group is generated.
(3) it after using aperture to collect mycelium pellet for the filtered through gauze of 0.8mm, is transferred on qualitative filter paper in 75 DEG C in baking oven
1h is completely dried to get Cordyceps Militaris animal feed 5.6g.Polysaccharide and protein therein are extracted using hot-water process, with phenol-sulphur
It is 9.2% that acid system, which measures its Cordyceps sinensis polysaccharide content, measures cordyceps sinensis protein content with Coomassie Brilliant Blue as 8.5%.With alkaline mistake
The remaining total nitrogen concentration that potassium sulfate resolution ultraviolet spectrophotometry measures water phase is 14mg/L, and corresponding nitrogen removal rate is
89.6%.Cultivated animals are directly fed with the mycelium pellet of drying.
The characteristics of this technique, is: suitable for carrying out the analysis to eutrophication water Transformation Potential, anticipation and condition optimizing,
It lays the foundation for subsequent large-scale engineering applications.
Embodiment 2
It (1) is 1.4m to volume3Reactive tank in injection containing cultivation fishpond water 1.2m3, wherein total nitrogen 2.8mg/L, total phosphorus
1.1mg/L.Glucose is added simultaneously to 550mg/L, and compares group with do not add glucose.
(2) the mycelium pellet suspension 120L of Cordyceps Militaris is inoculated with into reactive tank, mycelium pellet weight in wet base is 24kg, continuous aeration
Dissolved oxygen concentration is kept to be greater than 3.0mg/L, at room temperature occasional drive 1d, during which the mycelium pellet is constantly absorbed and utilized from water phase
Carbon source and nitrogen phosphorus etc. generate new mycelium pellet；Simultaneously it has been observed that due to a lack of carbon source, the only a small amount of new mycelium pellet of control group is produced
It is raw.
(3) it after collecting mycelium pellet using filter bag (diameter 50cm, depth 60cm, aperture 0.8mm), seeps and is done to anhydrous naturally
It oozes to get fresh new Cordyceps Militaris animal feed 180kg.Polysaccharide and protein therein are extracted using hot-water process, with phenolsulfuric acid
It is 10.2% that method, which measures its Cordyceps sinensis polysaccharide content, measures cordyceps sinensis protein content with Coomassie Brilliant Blue as 9.0%.With alkaline over cure
It is 0.58mg/L that sour potassium resolution ultraviolet spectrophotometry, which measures the remaining total nitrogen concentration of water phase, and corresponding nitrogen removal rate is
79.3%；Measure the remaining total phosphorus concentration of water phase with alkaline potassium per-sulfate digestion ammonium molybdate spectrophotometric method as 0.10mg/L, it is corresponding
Total tp removal rate is 90.9%.Cultivated animals are fed again after fresh new mycelium pellet is mixed into feed.
The characteristics of this technique is: the scale for realizing the Eutrophication materials of water body recycles and animal feed
Produce in enormous quantities, while providing Construction and operation parameter for other application engineering.
The above-mentioned description to embodiment is for that can understand and apply the invention convenient for those skilled in the art.
Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein general
Principle is applied in other embodiments without having to go through creative labor.Therefore, the present invention is not limited to embodiments here, originally
Field technical staff announcement according to the present invention, the improvement made for the present invention and modification all should be in protection models of the invention
Within enclosing.
Claims (10)
1. it is a kind of with carbon source promote can feeding fungal transformation water eutrophication substance method, which is characterized in that including as follows
Step:
(1) it is packed into the water body containing Eutrophication materials in the bioreactor, while adding carbon source；
(2) into bioreactor inoculation can feeding fungi mycelium pellet or mycelia block, 1~3d of occasional drive or company at room temperature
Continuous culture, makes Eutrophication materials assimilate into the component part for the mycelium pellet being newly proliferated；
(3) it collects the mycelium pellet being newly proliferated and is used to prepare animal feed.
2. the method according to claim 1, wherein in the step (1), bioreactor include triangular flask,
Fermentor or reactive tank, the bioreactor are also configured with agitating device or aerator, the stirring speed of the agitating device
Degree is 20~200 revs/min, and the aerator makes dissolved oxygen in water body reach 2~8mg/L.
3. the method according to claim 1, wherein in the step (1), Eutrophication materials include ammonia nitrogen,
Nitrate nitrogen and itrogenous organic substance and phosphate and organic phosphorus, the Water constituent content containing Eutrophication materials is 2~
1000mg N/L, phosphorus element content are 0.1~100mg P/L.
4. the method according to claim 1, wherein carbon source is glucose, sucrose, malt in the step (1)
Essence and molasses and other processing that can be analyzed to monosaccharide, the natural organic matter of disaccharides and small molecule carbohydrate or natural organic matter produce
Object, the carbon source amount of addition are equivalent to 0.02~20g/L glucose.
5. the method according to claim 1, wherein in the step (2), can feeding fungi include filamentous fungi
And macro fungi.
6. according to the method described in claim 5, it is characterized in that, it is described can feeding fungi be entomogenous fungi.
7. the method according to claim 1, wherein mycelium pellet is fungi in Liquid Culture in the step (2)
The mycelium pellet formed in base；Mycelia block is the mycelia block of fungi picking on solid culture primary surface, mycelium pellet or mycelia block with
In 0.4%~5% inoculum concentration access bioreactor.
8. the method according to claim 1, wherein occasional drive refers to bioreactor in the step (2)
To fill a water, a water is arranged, is so repeated；The continuous culture refers to that bioreactor is constantly water inlet and constantly row
Water.
9. the method according to claim 1, wherein the mycelium pellet being newly proliferated is using aperture in the step (3)
The gauze or filter bag of 0.2~2mm is collected, and the new mycelium pellet yield that is proliferated is 10~500g/L.
10. application of the mycelium pellet product on animal feed made from -9 described in any item methods according to claim 1, this is answered
Cultivated animals are fed with the mycelium pellet being newly proliferated with including directly, or are fed again after the mycelium pellet being newly proliferated is mixed into feed feeding
Grow animal.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000334488A (en) * | 1999-05-31 | 2000-12-05 | Able Corp | Device and method for water purification |
| CN101549936A (en) * | 2009-05-04 | 2009-10-07 | 清华大学 | A method using white-rot fungus and carrier to process persistent wastewater |
| CN102079612A (en) * | 2010-12-20 | 2011-06-01 | 张肇宏 | Lake eutrophication remediation method |
| CN102515339A (en) * | 2011-11-02 | 2012-06-27 | 中国水产科学研究院珠江水产研究所 | Culturing method of biological flocculation for aquiculture |
| CN105293715A (en) * | 2015-07-03 | 2016-02-03 | 重庆大学 | Method for controlling water body eutrophication by utilizing immobilized white-rot fungi, and application of method |
-
2018
- 2018-08-10 CN CN201810909765.5A patent/CN108996705A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000334488A (en) * | 1999-05-31 | 2000-12-05 | Able Corp | Device and method for water purification |
| CN101549936A (en) * | 2009-05-04 | 2009-10-07 | 清华大学 | A method using white-rot fungus and carrier to process persistent wastewater |
| CN102079612A (en) * | 2010-12-20 | 2011-06-01 | 张肇宏 | Lake eutrophication remediation method |
| CN102515339A (en) * | 2011-11-02 | 2012-06-27 | 中国水产科学研究院珠江水产研究所 | Culturing method of biological flocculation for aquiculture |
| CN105293715A (en) * | 2015-07-03 | 2016-02-03 | 重庆大学 | Method for controlling water body eutrophication by utilizing immobilized white-rot fungi, and application of method |
Non-Patent Citations (1)
| Title |
|---|
| 张利民等: "《水域营养生态学》", 31 July 2012, 海洋出版社 * |
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