CN108949593A - A kind of compound micro-ecological preparation and its preparation method and application - Google Patents
A kind of compound micro-ecological preparation and its preparation method and application Download PDFInfo
- Publication number
- CN108949593A CN108949593A CN201810927220.7A CN201810927220A CN108949593A CN 108949593 A CN108949593 A CN 108949593A CN 201810927220 A CN201810927220 A CN 201810927220A CN 108949593 A CN108949593 A CN 108949593A
- Authority
- CN
- China
- Prior art keywords
- culture
- compound micro
- parts
- ecological preparation
- ecological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Mycology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Mechanical Engineering (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of probiotics more particularly to a kind of compound micro-ecological preparation and its preparation method and application.The compound micro-ecological preparation includes the component of following parts by weight: 25-40 parts of saccharomycete, 10-25 parts of bacillus subtilis, 15-35 parts of bacillus licheniformis, 10-25 parts of actinomyces, 45-70 parts of carrier.Compound micro-ecological preparation of the invention can be applied in facilities vegetable planting process; beneficial microbe can be made to obtain mass propagation near root system of plant; protect the vigorous growth of root system; root system is helped to resist the harm of harmful pathogen; inhibit harmful microbe growth and breeding in soil; the soil continuous cropping obstacle for effectively overcoming facilities vegetable plant, improves the quality and yield of facilities vegetable.
Description
(1) technical field
The present invention relates to a kind of probiotics more particularly to a kind of compound micro-ecological preparation and preparation method and application.
(2) background technique
Vegetable production in green house is the main production method of current vegetables industry, to solution vegetables year-round supply, kind surplus and deficiency of adjusting
Particularly important effect is played, is a kind of planting industry production method that height is intensive, it has also become agricultural and rural economy hair
One of leading industry of exhibition.With the rapid development of China's agricultural, the plantation of facilities vegetable has obtained the popularization of large area.But
It is influenced since vegetables produce by multiple external conditions such as soil, illumination, moisture, especially in recent years, China various regions facility vegetable
Colza plants the phenomenon that all suffering from soil continuous cropping obstacle, especially with China's vegetable production in green house scale, industrialization, specialization
Development largely affect the sustainable development of China's facilities vegetable so that soil continuous cropping obstacle is more serious.
For facilities vegetable under the conditions of sequential cropping cultivation, suitable temperature and humidity conditions can be certain pathogens, harmful micro- life for a long time
Object etc. provides advantageous Reproduction Conditions, is continuously increased the quantity of pathogenic microorganism in soil.Secondly, pesticide, chemical fertilizer is a large amount of
It uses, the balance of ecological environment of soil can be also destroyed, to the nitrogen-fixing bacteria in the microbial population or even soil in soil, root nodule
The beneficial microbes such as bacterium, cause of disease Antagonistic Fungi and organic matter decomposition bacterium have adverse effect on.
The long-term continuous cropping of facilities vegetable will lead to the destruction of soil ecology balance, noxious material accumulation, content of organic matter decline, disease
The problems such as opportunistic pathogen increasing number, microelement are depleted, acid-base property is unbalance.Probiotics are the fingers in microecology theory
It leads down, adjustment ecological disturbance keeps microecological balance, improves the physiological of host health level or the state that improves health in environment
Viable bacteria product and its metabolite, and the substance product for promoting these physiology flora growths to breed.Probiotics have been at present
Applied to breeding ecological environment in water environment treatment, can be effectively improved, the pollutions, enhancing such as Water quality, elimination ammonia nitrogen are adjusted
The immunity for cultivating object, plays highly important effect in healthy aquaculture.Probiotics also have conditioning soil,
Soil hardening is eliminated, promotees to develop the roots of the crops, grows the microbial population beneficial to plant growth quickly in the soil
The advantages that breeding, decomposes the harmful substance of plant root exudation, raising crops quality and yield, free from environmental pollution,
So probiotics are of great significance applied to agricultural.
(3) summary of the invention
In order to compensate for the shortcomings of the prior art, the present invention provides a kind of compound micro-ecological preparations and its preparation method and application.
The present invention is achieved through the following technical solutions:
A kind of compound micro-ecological preparation, which is characterized in that the component including following parts by weight: 25-40 parts of saccharomycete, withered grass bud
10-25 parts of spore bacillus, 15-35 parts of bacillus licheniformis, 10-25 parts of actinomyces, 45-70 parts of carrier.
The training method of the saccharomycete are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, it is used
Culture medium contains the ingredient of following weight percent: glucose 1.5-2.0%, corn starch 1.2-2. 2%, Yeast protein 0.5-
1. 0%, soy meal 0.2-0. 6%, dipotassium hydrogen phosphate 0.2-0.5%, sodium chloride 0.02-0.08%, magnesium sulfate 0.01-0.02%, sulphur
Sour ammonium 0.01-0.03%, remaining is water, and pH=6.7-7.5, condition of culture is 28-36 DEG C, aerlbic culture 36-72 hours.
The training method of the bacillus subtilis are as follows: train bacterial strain by the sequence of inclined-plane, liquid seeds, liquid fermentation
It supports, used medium contains the ingredient of following weight percent: glucose 0.8-1. 2%, corn starch 0.3-0.8%, yeast
Albumen 0.3-0.6%, maize flour 0.1-0. 2%, dipotassium hydrogen phosphate 0.02-0.06%, 0. 01-0.02% of magnesium sulfate, ammonium sulfate
0.01-0.03%, manganese sulfate 0.005-0.008%, remaining is water, and pH=6.8-7.5, condition of culture is 28-38 DEG C, cultivates 36-
72 hours.
The training method of the bacillus licheniformis are as follows: train bacterial strain by the sequence of inclined-plane, liquid seeds, liquid fermentation
It supports, used culture medium contains the ingredient of following weight percent: glucose 0.8-1.2%, corn starch 0.3-0.8%, ferment
Female albumen 0.3-0.6%, maize flour 0.1-0. 2%, dipotassium hydrogen phosphate 0.02-0.06%, magnesium sulfate 0.01-0.02%, ammonium sulfate
0.01-0.03%, manganese sulfate 0.005-0.008%, remaining is water, and pH=6.8-7.5, condition of culture is 28-38 DEG C, cultivates 36-
48 hours.
The training method of the actinomyces are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, grape
Sugared 0.8-1.2%, corn starch 0.3-0.8%, Yeast protein 0.3-0.8%, maize flour 0.1-0.3%, dipotassium hydrogen phosphate 0.02-
0.08%, magnesium sulfate 0.01-0.03%, ammonium sulfate 0.01-0.02%, remaining is water, pH=6.8-7.5, condition of culture 28-38
DEG C, aerlbic culture 36-72 hours.
The carrier is to have one of diatomite, plant ash, humic acid, corn stover or a variety of.
The preparation method of compound micro-ecological preparation, it is characterised in that: by saccharomycete, bacillus subtilis, lichens gemma bar
Bacterium, actinomyces and carrier mix well, and pack after low temperature drying, control total viable count 8,000,000,000 or more.
The compound micro-ecological preparation can be applied to agricultural soil and administer and facilities vegetable growing industry.
The beneficial effects of the present invention are: compound micro-ecological preparation of the invention selects safety, excellent strain, assisted between bacterial strain
It is good to make Symbiotic effectiveness, viable bacteria concentration is high in compound micro-ecological preparation, long shelf-life, and using effect is significant.
Compound micro-ecological preparation of the invention can be applied in facilities vegetable planting process, and beneficial microbe can be made to plant
Object root system nearby obtains mass propagation, protects the vigorous growth of root system, helps root system to resist the harm of harmful pathogen, inhibits soil
Harmful microbe growth and breeding in earth.Meanwhile the formation of soil aggregate structure can be promoted, so that soil is become more loose,
Enhance soil liquid manure retentivity, effectively enhancing the soil water, fertilizer, air and heat harmony, for vegetable plant strain grow more preferable soil is provided
Earth environment effectively overcomes the soil continuous cropping obstacle of facilities vegetable plant, improves the quality and yield of facilities vegetable.
(4) specific embodiment
Invention is further described in detail combined with specific embodiments below.
Embodiment 1
Compound micro-ecological preparation, the component including following parts by weight: 25 parts of saccharomycete, 10 parts of bacillus subtilis, lichens bud
15 parts of spore bacillus, 10 parts of actinomyces, diatomite and 45 parts of plant ash mixture.
The training method of saccharomycete are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, culture used
Base contains the ingredient of following weight percent: glucose 1.5%, corn starch 1.2%, Yeast protein 0.5%, soy meal 0.2%, phosphorus
Sour hydrogen dipotassium 0.2%, sodium chloride 0.08%, magnesium sulfate 0.02%, ammonium sulfate 0.01%, remaining is water, and pH=6.7-7.5 cultivates item
Part is 28 DEG C, aerlbic culture 36 hours.
The training method of bacillus subtilis are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, institute
Contain the ingredient of following weight percent: glucose 0.8%, corn starch 0.8%, Yeast protein 0.3%, maize flour with culture medium
0.1%, dipotassium hydrogen phosphate 0. 06%, magnesium sulfate 0.02%, ammonium sulfate 0.01%, manganese sulfate 0.005%, remaining is water, pH=6.8-
7.5, condition of culture is 28 DEG C, is cultivated 72 hours.
The training method of bacillus licheniformis are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, institute
The culture medium used contains the ingredient of following weight percent: glucose 1.2%, corn starch 0.8%, Yeast protein 0.6%, jade
Rice and flour 0. 2%, dipotassium hydrogen phosphate 0.06%, magnesium sulfate 0.01%, ammonium sulfate 0.01%, manganese sulfate 0.005%, remaining is water, pH=
6.8-7.5, condition of culture are 28 DEG C, are cultivated 48 hours.
The training method of actinomyces are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, glucose 1.
2%, corn starch 0.8%, Yeast protein 0.3%, maize flour 0.3%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.01%, ammonium sulfate
0.01%, remaining is water, and pH=6.8-7.5, condition of culture is 28 DEG C, aerlbic culture 72 hours.
Compound micro-ecological preparation the preparation method comprises the following steps: by saccharomycete, bacillus subtilis, bacillus licheniformis, actinomyces
It mixes well with diatomite, plant ash, is packed after dry under the conditions of 35-45 DEG C, control total viable count in 8,000,000,000/g or more.
Embodiment 2
Compound micro-ecological preparation, the component including following parts by weight: 40 parts of saccharomycete, 25 parts of bacillus subtilis, lichens bud
35 parts of spore bacillus, 25 parts of actinomyces, diatomite and 60 parts of humic acid mixture.
Compound micro-ecological preparation the preparation method comprises the following steps: by saccharomycete, bacillus subtilis, bacillus licheniformis, actinomyces
It mixes well with diatomite, humic acid, is packed after dry under the conditions of 35-45 DEG C, control total viable count in 8,000,000,000/g or more.
Remaining is same as Example 1.
Embodiment 3
Compound micro-ecological preparation, the component including following parts by weight: 30 parts of saccharomycete, 20 parts of bacillus subtilis, lichens bud
25 parts of spore bacillus, 20 parts of actinomyces, 70 parts of mixture of humic acid and corn stover.
Compound micro-ecological preparation the preparation method comprises the following steps: by saccharomycete, bacillus subtilis, bacillus licheniformis, actinomyces
It mixes well with humic acid, corn stover, is packed after dry under the conditions of 35-45 DEG C, control total viable count in 8,000,000,000/g or more.
Embodiment 4
The training method of saccharomycete are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, used medium contains
There is the ingredient of following weight percent: glucose 2.0%, corn starch 2.2%, Yeast protein 1.0%, soy meal 0.6%, phosphoric acid hydrogen
Dipotassium 0.5%, sodium chloride 0.02%, magnesium sulfate 0.01%, ammonium sulfate 0.01%, remaining is water, pH=6.7-7.5, and condition of culture is
36 DEG C, aerlbic culture 72 hours.
The training method of bacillus subtilis are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, institute
Contain the ingredient of following weight percent: glucose 1. 2%, corn starch 0.3%, Yeast protein 0.6%, corn with culture medium
Face 0.2%, dipotassium hydrogen phosphate 0.06%, magnesium sulfate 0. 01%, ammonium sulfate 0.03%, manganese sulfate 0.008%, remaining is water, pH=
6.8-7.5, condition of culture are 36 DEG C, are cultivated 48 hours.
The training method of bacillus licheniformis are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, institute
The culture medium used contains the ingredient of following weight percent: glucose 0.8%, corn starch 0.3%, Yeast protein 0.3%, jade
Rice and flour 0.1%, dipotassium hydrogen phosphate 0.02%, magnesium sulfate 0.01%, ammonium sulfate 0.01%, manganese sulfate 0.005%, remaining is water, pH=
6.8-7.5, condition of culture are 36 DEG C, are cultivated 36 hours.
The training method of actinomyces are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, glucose
0.8%, corn starch 0.3%, Yeast protein 0.3%, maize flour 0.2%, dipotassium hydrogen phosphate 0.07%, magnesium sulfate 0.03%, ammonium sulfate
0.02%, remaining is water, and pH=6.8-7.5, condition of culture is 38 DEG C, aerlbic culture 36 hours.
Remaining is same as Example 1.
It is further described below with reference to experiment.
Plot: 2 mu of Jiyang Qu Dizhen cucumber canopy is tested, cucumber canopy is 12 years continuous planting sheds.The plot is divided into improvement
Cucumber seedling is transplanted in group 1, four pieces of improvement group 2, improvement group 3 and control group plot, four plot simultaneously, and improvement group 1-3 applies in cave compound micro-
Ecological agent, control group do not use compound micro-ecological preparation, and other conditions are identical as improvement group 1-3.
One, when cucumber is colonized 18 days, 30 days, 45 days, the topsoil soils of cucumber root 0-20cm are acquired respectively, are pressed " Z "
Row method determines 5 sampled points and acquires mixed soil sample, and testing result is as shown in table 1.
1 compound micro-ecological preparation of table influences plastic tent cucumber continuous cropping soil physicochemical property
As can be seen from Table 1, compound micro-ecological preparation has apparent improved effect to greenhouse continuous cropping soil, can regulate and control soil pH
Value, significantly improve soil organic matter content, be effectively improved the soil salinization, promote to a certain extent soil in protected field nitrogen, phosphorus,
Effective accumulation of potassium.
Two, 18 days, 30 days, 45 days, separately sampled measurement cucumber plant items growth indexes, as a result such as table are colonized in cucumber
Shown in 2.
Influence of 2 compound micro-ecological preparation of table to cucumber growth index
As can be seen from Table 2, compound micro-ecological preparation can effectively facilitate cucumber plant growth, and improvement group cucumber growth gesture is more right
It is significantly increased according to group, especially cucumber growth later period performance is more prominent.
Three, after cucumber enters harvest time, all picking is completed to calculate each processing yield, and the results are shown in Table 3.
Influence of 3 compound micro-ecological preparation of table to cucumber yield
As can be seen from Table 3, every plant of fruit number of two groups of processing does not have notable difference, but improvement group total output is significantly higher than control
Group, compound micro-ecological preparation have significant ground facilitation to cucumber yield.
Four, when cucumber customizes 45 days, 52 days, 59 days, 64 days, 69 days, the fixed point choosing at random in improvement group and control group
Taking 20 plants of investigation downy mildew, a situation arises, and the investigation radix of wilt disease is all plant in the group, as a result such as 5 institute of table 4 and table
Show.
Wilt disease and downy mildew disease incidence=disease plant number/investigation total strain number * 100%
Influence of 4 compound micro-ecological preparation of table to cucumber fusarium axysporum
Ascendant trend is all presented in the withered water chestnut disease disease incidence respectively handled as can be seen from Table 4.The wilt disease disease incidence of control group is whole
Higher level is constantly in a growth period, and speedup is very fast；The cucumber fusarium axysporum disease incidence of improvement group 1-3 is lower, is growing
Late onset rate is increased with the amplitude of very little.It can be seen that can effective lower cucumber fusarium axysporum using compound micro-ecological preparation
Generation, slow down wilt disease onset speed.
Influence of 5 compound micro-ecological preparation of table to cucumber downy mildew
The disease incidence presentation of cucumber downy mildew, which first increases afterwards, as can be seen from Table 5 reduces later raised trend again, in field planting 59
It when each group downy mildew disease incidence reach highest.The disease incidence of improvement group 1-3 downy mildew within cucumber entire growth period is bright
It is aobvious to be lower than control group, illustrate that compound micro-ecological preparation can mitigate the generation of cucumber downy mildew to a certain extent.
Embodiment described above, only several, the those skilled in the art of the present invention more preferably specific embodiment
The usual variations and alternatives that member carries out within the scope of technical solution of the present invention should be all included within the scope of the present invention.
Claims (8)
1. a kind of compound micro-ecological preparation, which is characterized in that the component including following parts by weight: 25-40 parts of saccharomycete, withered grass
10-25 parts of bacillus, 15-35 parts of bacillus licheniformis, 10-25 parts of actinomyces, 45-70 parts of carrier.
2. compound micro-ecological preparation according to claim 1, it is characterised in that: the training method of the saccharomycete are as follows: will
Bacterial strain presses the sequence culture on inclined-plane, liquid seeds, liquid fermentation, and used medium contains the ingredient of following weight percent: Portugal
Grape sugar 1.5-2.0%, corn starch 1.2-2. 2%, Yeast protein 0.5-1. 0%, soy meal 0.2-0. 6%, dipotassium hydrogen phosphate
0.2-0.5%, sodium chloride 0.02-0.08%, magnesium sulfate 0.01-0.02%, ammonium sulfate 0.01-0.03%, remaining is water, pH=6.7-
7.5, condition of culture is 28-36 DEG C, aerlbic culture 36-72 hours.
3. compound micro-ecological preparation according to claim 1, it is characterised in that: the training method of the bacillus subtilis
Are as follows: by bacterial strain press inclined-plane, liquid seeds, liquid fermentation sequence culture, used medium contain following weight percent at
Point: glucose 0.8-1. 2%, corn starch 0.3-0.8%, Yeast protein 0.3-0.6%, maize flour 0.1-0. 2%, phosphoric acid hydrogen
0. 02-0. 06% of dipotassium, 0. 01-0.02% of magnesium sulfate, ammonium sulfate 0.01-0.03%, manganese sulfate 0.005-0.008%, remaining is
Water, pH=6.8-7.5, condition of culture are 28-38 DEG C, are cultivated 36-72 hours.
4. compound micro-ecological preparation according to claim 1, it is characterised in that: the training method of the bacillus licheniformis
Are as follows: bacterial strain is pressed to the sequence culture on inclined-plane, liquid seeds, liquid fermentation, used culture medium contains following weight percent
Ingredient: glucose 0.8-1.2%, corn starch 0.3-0.8%, Yeast protein 0.3-0.6%, maize flour 0.1-0. 2%, phosphoric acid
Hydrogen dipotassium 0.02-0.06%, magnesium sulfate 0.01-0.02%, ammonium sulfate 0.01-0.03%, manganese sulfate 0.005-0.008%, remaining is
Water, pH=6.8-7.5, condition of culture are 28-38 DEG C, are cultivated 36-48 hours.
5. compound micro-ecological preparation according to claim 1, it is characterised in that: the training method of the actinomyces are as follows: will
Bacterial strain presses the sequence culture on inclined-plane, liquid seeds, liquid fermentation, glucose 0.8-1. 2%, corn starch 0.3-0.8%, yeast
Albumen 0.3-0.8%, maize flour 0.1-0.3%, 0. 02-0. 08% of dipotassium hydrogen phosphate, 0. 01-0.03% of magnesium sulfate, ammonium sulfate
0.01-0.02%, remaining is water, and pH=6.8-7.5, condition of culture is 28-38 DEG C, aerlbic culture 36-72 hours.
6. compound micro-ecological preparation according to claim 1, it is characterised in that: the carrier be have diatomite, plant ash,
One of humic acid, corn stover are a variety of.
7. a kind of preparation method of compound micro-ecological preparation as described in claim 1, it is characterised in that: by saccharomycete, withered grass
Bacillus, bacillus licheniformis, actinomyces and carrier mix well, and pack after low temperature drying, control total viable count 8,000,000,000
More than.
8. a kind of application of compound micro-ecological preparation as described in claim 1, it is characterised in that: the compound micro-ecological preparation
It is administered and facilities vegetable growing industry applied to agricultural soil.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927220.7A CN108949593A (en) | 2018-08-15 | 2018-08-15 | A kind of compound micro-ecological preparation and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810927220.7A CN108949593A (en) | 2018-08-15 | 2018-08-15 | A kind of compound micro-ecological preparation and its preparation method and application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108949593A true CN108949593A (en) | 2018-12-07 |
Family
ID=64469064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810927220.7A Pending CN108949593A (en) | 2018-08-15 | 2018-08-15 | A kind of compound micro-ecological preparation and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108949593A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467508A (en) * | 2019-08-01 | 2019-11-19 | 山东亿农高科生物科技有限公司 | A kind of facility cultivation acidified soil microorganism renovation agent and preparation method thereof |
| CN111944726A (en) * | 2020-08-24 | 2020-11-17 | 广州市土根旺生物科技有限公司 | Soil microbial population regulator and preparation method and application thereof |
| CN112409065A (en) * | 2020-11-19 | 2021-02-26 | 中国农业科学院草原研究所 | Soil finishing agent for overcoming continuous cropping obstacle of potatoes and application method thereof |
| CN114891514A (en) * | 2022-04-15 | 2022-08-12 | 西南石油大学 | Shale gas development platform soil reclamation enhancer and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
| CN101993829A (en) * | 2009-08-25 | 2011-03-30 | 吉林师范大学 | Composite microbial agent |
| US20170081631A1 (en) * | 2015-09-17 | 2017-03-23 | Richard H. Maguire | Rapid Growth Activator |
| CN106631204A (en) * | 2016-11-16 | 2017-05-10 | 云南金凯利生物有机肥业有限公司 | Multifunctional organic fertilizer and preparation method thereof |
-
2018
- 2018-08-15 CN CN201810927220.7A patent/CN108949593A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101508601A (en) * | 2009-03-31 | 2009-08-19 | 孙明坤 | Microorganism organic fertilizer and method of preparing the same |
| CN101993829A (en) * | 2009-08-25 | 2011-03-30 | 吉林师范大学 | Composite microbial agent |
| US20170081631A1 (en) * | 2015-09-17 | 2017-03-23 | Richard H. Maguire | Rapid Growth Activator |
| CN106631204A (en) * | 2016-11-16 | 2017-05-10 | 云南金凯利生物有机肥业有限公司 | Multifunctional organic fertilizer and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 何若天 等: "《农用益生菌生产与应用手册》", 28 February 2016, 金盾出版社 * |
| 科学技术部农村科技司组编: "《次生代谢在生态农业中的应用》", 31 March 2018, 中国农业大学出版社 * |
| 郑亚南等: "喷施堆肥复合微生物菌剂条件下草坪植物萌发及生长特性", 《种子》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110467508A (en) * | 2019-08-01 | 2019-11-19 | 山东亿农高科生物科技有限公司 | A kind of facility cultivation acidified soil microorganism renovation agent and preparation method thereof |
| CN111944726A (en) * | 2020-08-24 | 2020-11-17 | 广州市土根旺生物科技有限公司 | Soil microbial population regulator and preparation method and application thereof |
| CN112409065A (en) * | 2020-11-19 | 2021-02-26 | 中国农业科学院草原研究所 | Soil finishing agent for overcoming continuous cropping obstacle of potatoes and application method thereof |
| CN114891514A (en) * | 2022-04-15 | 2022-08-12 | 西南石油大学 | Shale gas development platform soil reclamation enhancer and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104844313B (en) | A kind of preparation method of selenium-rich cherry and tomato bio-organic fertilizer special | |
| CN101126026B (en) | Vegetable field soil improving agent and its preparation method | |
| CN103992184B (en) | A kind of biological organic fertilizer | |
| CN109485498A (en) | A kind of method of preparation and use of high organic complex microorganism flushing and spreading fertilizer | |
| CN106386163A (en) | Method for cultivating edible fungi by using crop straw | |
| CN108949593A (en) | A kind of compound micro-ecological preparation and its preparation method and application | |
| CN102888356B (en) | Bacillus subtilis, microbial fertilizer prepared by using bacillus subtilis, method for preparing microbial fertilizer by using bacillus subtilis, and application of microbial fertilizer | |
| CN101372425A (en) | Microbial fertilizer and preparation thereof | |
| CN108029501A (en) | A kind of vegetable nutrient diseases prevention seedling medium and preparation method and application | |
| CN106007824B (en) | Composite bacterial fertilizer and preparation method and application thereof | |
| CN107188724B (en) | Fermentation process of biological organic bacterial fertilizer | |
| CN107418906B (en) | A kind of composite bacteria agent and its application improving color green pepper yield and anti-root rot | |
| CN110591937B (en) | Antagonistic actinomycetes and biological organic fertilizer for preventing and controlling tomato bacterial wilt, method and application | |
| CN110218678B (en) | Anti-continuous cropping soil remediation agent and preparation method thereof | |
| CN101928161B (en) | Method for preparing direct seeding compression nutrition pot raw material | |
| CN107926607A (en) | A kind of lichee sapling culture medium and preparation method thereof | |
| CN107285953A (en) | Composite zymocyte, biological organic fertilizer and preparation method thereof | |
| Abdelmoaty et al. | Influence of Trichoderma harzianum and Bacillus thuringiensis with reducing rates of NPK on growth, physiology, and fruit quality of Citrus aurantifolia | |
| CN106396862A (en) | Disease controlling microbial fertilizer special for tuberous crops and preparation method thereof | |
| CN105315068A (en) | Composition for promoting crop growth and preparation method and fertilization method thereof | |
| CN104844285A (en) | Method for preparing bio-organic fertilizer for improving immunity of cherry tomatoes | |
| CN104817366B (en) | A kind of preparation method for the biological organic fertilizer that can improve Fuji apple storage quality | |
| CN105766595A (en) | Organic planting method of dendrobium officinale | |
| CN111727847B (en) | Straw fiber seedling culture substrate for colonization biocontrol bacteria, production method thereof and two-step colonization biocontrol bacteria method | |
| CN107602278A (en) | The preparation method of tobacco breeding and seedling nursing with equipment matrix |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181207 |